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Sequence NM_001018109
CCTCCCGCCTCCTCTAGGCCGCCGGCCGCGAAGCGCTGAGTCACGGTGAGGCTACTGGACCCACACTCTCTTAACCTGCCCTCCCTGCACTCGCTCCCGGCGGCTCTTCGCGTCACCCCCGCCGCTAAGGCTCCAGGTGCCGCTACCGCAGCCCCTCCATCCTCTACAGCTCAGCATCAGAACACTCTCTTTTTAGACTC
CGATATGGGGTCCTCCAAGAAAGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCGGAAGGGGTTGGAGCGAGGGTCCGGAGAAGCATTGGCAGACCCGAG
TTAAAAAATCTGGATCCGTTTTTACTGTTTGATGAATTTAAAGGAGGTAGACCAGGAGGATTTCCTGATCATCCACATCGAGGTTTTGAAACAGTATCCT
ACCTCCTGGAAGGGGGCAGCATGGCCCATGAAGACTTCTGTGGACACACTGGTAAAATGAACCCAGGAGATTTGCAGTGGATGACTGCGGGCCGGGGCAT
TCTGCACGCTGAGATGCCTTGCTCAGAGGAGCCAGCCCATGGCCTACAACTGTGGGTTAATTTGAGGAGCTCAGAGAAGATGGTGGAGCCTCAGTACCAG
GAACTGAAAAGTGAAGAAATCCCTAAACCCAGTAAGGATGGTGTGACAGTTGCTGTCATTTCTGGAGAAGCCCTGGGAATAAAGTCCAAGGTTTACACTC
GCACACCAACCTTATATTTGGACTTCAAATTGGACCCAGGAGCCAAACATTCCCAACCTATCCCTAAAGGGTGGACAAGCTTCATTTACACGATATCTGG
AGATGTGTATATTGGGCCCGATGATGCACAACAAAAAATAGAACCTCATCACACAGCAGTGCTTGGAGAAGGTGACAGTGTCCAGGTGGAGAACAAGGAT
CCCAAGAGAAGCCACTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTATCCAACATGGTCCATTTGTGATGAACACCAATGAAGAGATTTCTC
AAGCTATTCTTGATTTCAGAAACGCAAAAAATGGGTTTGAAAGGGCCAAAACCTGGAAATCAAAGATTGGGAACTAGTGGAAAGCGGAAGAGCAGGTCTT
GATGTGTCCTAGAATTTTGCCATTTCTGAGATTGAGCCATTGAAGGCATTCCATTTCTAAAGCTTATTTAGCCGGTGCTTCTAAAGAATTCCACACTAAC
GTGATAACATGGTTTTTGTAACAATAAATGTAGGATATTTCCTGGCACATGCAAATAAACCTAATCATTGTTTCTTTAAAAA
- refseq_PIR.F1 (forward primer)
- FIGF.R4 (reverse primer)
- Alternative donor
Frame 0
PPASSRPPAAKR*VTVRLLDPHSLNLPSLHSLPAALRVTPAAKAPGAATAAPPSSTAQHQNTLFLDS
DMGSSKKVTLSVLSREQSEGVGARVRRSIGRPE
LKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVS
YLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGI
LHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQ
ELKSEEIPKPSKDGVTVAVISGEALGIKSKVYT
RTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISG
DVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKD
PKRSHFVLIAGEPLREPVIQHGPFVMNTNEEIS
QAILDFRNAKNGFERAKTWKSKIGN*WKAEEQVL
MCPRILPFLRLSH*RHSISKAYLAGASKEFHTN
VITWFL*Q*M*DISWHMQINLIIVSLK
Frame 1
LPPPLGRRPRSAESR*GYWTHTLLTCPPCTRSRRLFASPPPLRLQVPLPQPLHPLQLSIRTLSF*T
PIWGPPRKLLSQCSAGSSRKGLERGSGEALADPS
*KIWIRFYCLMNLKEVDQEDFLIIHIEVLKQYP
TSWKGAAWPMKTSVDTLVK*TQEICSG*LRAGA
FCTLRCLAQRSQPMAYNCGLI*GAQRRWWSLSTR
N*KVKKSLNPVRMV*QLLSFLEKPWE*SPRFTL
AHQPYIWTSNWTQEPNIPNLSLKGGQASFTRYL
EMCILGPMMHNKK*NLITQQCLEKVTVSRWRTRI
PREATLS*LLGSH*ENQLSNMVHL**TPMKRFL
KLFLISETQKMGLKGPKPGNQRLGTSGKRKSRS
*CVLEFCHF*D*AIEGIPFLKLI*PVLLKNSTLT
**HGFCNNKCRIFPGTCK*T*SLFL*K
Frame 2
SRLL*AAGREALSHGEATGPTLS*PALPALAPGGSSRHPRR*GSRCRYRSPSILYSSASEHSLFRL
RYGVLQESYSLSAQPGAVGRGWSEGPEKHWQTR
VKKSGSVFTV**I*RR*TRRIS*SSTSRF*NSIL
PPGRGQHGP*RLLWTHW*NEPRRFAVDDCGPGH
SAR*DALLRGASPWPTTVG*FEELREDGGASVP
GTEK*RNP*TQ*GWCDSCCHFWRSPGNKVQGLHS
HTNLIFGLQIGPRSQTFPTYP*RVDKLHLHDIW
RCVYWAR*CTTKNRTSSHSSAWRR*QCPGGEQG
SQEKPLCLNCWGAIKRTSYPTWSICDEHQ*RDFS
SYS*FQKRKKWV*KGQNLEIKDWELVESGRAGL
DVS*NFAISEIEPLKAFHF*SLFSRCF*RIPH*
RDNMVFVTINVGYFLAHANKPNHCFFK
Sequence NM_003662
CCTCCCGCCTCCTCTAGGCCGCCGGCCGCGAAGCGCTGAGTCACGGTGAGGCTACTGGACCCACACTCTCTTAACCTGCCCTCCCTGCACTCGCTCCCGGCGGCTCTTCGCGTCACCCCCGCCGCTAAGGCTCCAGGTGCCGCTACCGCAGCGTGAGTACCTGGGGCTCCTGCAGGGGTCCACTAGCCCTCCATCCTCTA
CAGCTCAGCATCAGAACACTCTCTTTTTAGACTCCGATATGGGGTCCTCCAAGAAAGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCGGAAGGGGTTGG
AGCGAGGGTCCGGAGAAGCATTGGCAGACCCGAGTTAAAAAATCTGGATCCGTTTTTACTGTTTGATGAATTTAAAGGAGGTAGACCAGGAGGATTTCCT
GATCATCCACATCGAGGTTTTGAAACAGTATCCTACCTCCTGGAAGGGGGCAGCATGGCCCATGAAGACTTCTGTGGACACACTGGTAAAATGAACCCAG
GAGATTTGCAGTGGATGACTGCGGGCCGGGGCATTCTGCACGCTGAGATGCCTTGCTCAGAGGAGCCAGCCCATGGCCTACAACTGTGGGTTAATTTGAG
GAGCTCAGAGAAGATGGTGGAGCCTCAGTACCAGGAACTGAAAAGTGAAGAAATCCCTAAACCCAGTAAGGATGGTGTGACAGTTGCTGTCATTTCTGGA
GAAGCCCTGGGAATAAAGTCCAAGGTTTACACTCGCACACCAACCTTATATTTGGACTTCAAATTGGACCCAGGAGCCAAACATTCCCAACCTATCCCTA
AAGGGTGGACAAGCTTCATTTACACGATATCTGGAGATGTGTATATTGGGCCCGATGATGCACAACAAAAAATAGAACCTCATCACACAGCAGTGCTTGG
AGAAGGTGACAGTGTCCAGGTGGAGAACAAGGATCCCAAGAGAAGCCACTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTATCCAACATGGT
CCATTTGTGATGAACACCAATGAAGAGATTTCTCAAGCTATTCTTGATTTCAGAAACGCAAAAAATGGGTTTGAAAGGGCCAAAACCTGGAAATCAAAGA
TTGGGAACTAGTGGAAAGCGGAAGAGCAGGTCTTGATGTGTCCTAGAATTTTGCCATTTCTGAGATTGAGCCATTGAAGGCATTCCATTTCTAAAGCTTA
TTTAGCCGGTGCTTCTAAAGAATTCCACACTAACGTGATAACATGGTTTTTGTAACAATAAATGTAGGATATTTCCTGGCACATGCAAATAAACCTAATC
ATTGTTTCTTTAAAAA
- refseq_PIR.F1 (forward primer)
- FIGF.R4 (reverse primer)
- Alternative donor
Frame 0
PPASSRPPAAKR*VTVRLLDPHSLNLPSLHSLPAALRVTPAAKAPGAATAA*VPGAPAGVH*PSILY
SSASEHSLFRLRYGVLQESYSLSAQPGAVGRGW
SEGPEKHWQTRVKKSGSVFTV**I*RR*TRRIS
*SSTSRF*NSILPPGRGQHGP*RLLWTHW*NEPR
RFAVDDCGPGHSAR*DALLRGASPWPTTVG*FE
ELREDGGASVPGTEK*RNP*TQ*GWCDSCCHFW
RSPGNKVQGLHSHTNLIFGLQIGPRSQTFPTYP*
RVDKLHLHDIWRCVYWAR*CTTKNRTSSHSSAW
RR*QCPGGEQGSQEKPLCLNCWGAIKRTSYPTW
SICDEHQ*RDFSSYS*FQKRKKWV*KGQNLEIKD
WELVESGRAGLDVS*NFAISEIEPLKAFHF*SL
FSRCF*RIPH*RDNMVFVTINVGYFLAHANKPN
HCFFK
Frame 1
LPPPLGRRPRSAESR*GYWTHTLLTCPPCTRSRRLFASPPPLRLQVPLPQREYLGLLQGSTSPPSS
TAQHQNTLFLDSDMGSSKKVTLSVLSREQSEGVG
ARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFP
DHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNP
GDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLR
SSEKMVEPQYQELKSEEIPKPSKDGVTVAVISG
EALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIP
KGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLG
EGDSVQVENKDPKRSHFVLIAGEPLREPVIQHG
PFVMNTNEEISQAILDFRNAKNGFERAKTWKSK
IGN*WKAEEQVLMCPRILPFLRLSH*RHSISKAY
LAGASKEFHTNVITWFL*Q*M*DISWHMQINLI
IVSLK
Frame 2
SRLL*AAGREALSHGEATGPTLS*PALPALAPGGSSRHPRR*GSRCRYRSVSTWGSCRGPLALHPL
QLSIRTLSF*TPIWGPPRKLLSQCSAGSSRKGL
ERGSGEALADPS*KIWIRFYCLMNLKEVDQEDFL
IIHIEVLKQYPTSWKGAAWPMKTSVDTLVK*TQ
EICSG*LRAGAFCTLRCLAQRSQPMAYNCGLI*
GAQRRWWSLSTRN*KVKKSLNPVRMV*QLLSFLE
KPWE*SPRFTLAHQPYIWTSNWTQEPNIPNLSL
KGGQASFTRYLEMCILGPMMHNKK*NLITQQCL
EKVTVSRWRTRIPREATLS*LLGSH*ENQLSNMV
HL**TPMKRFLKLFLISETQKMGLKGPKPGNQR
LGTSGKRKSRS*CVLEFCHF*D*AIEGIPFLKL
I*PVLLKNSTLT**HGFCNNKCRIFPGTCK*T*S
LFL*K
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