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Sequences

Sequence NM_145320

GCTGGTGGCGCCCGCCAGCCGGCCCGGGAGAGGGGACTTGAGCGCTTCGCGGCCGGTGCGCAGCGCGGCTCCGGCCCCGCAGTCTGTCTGCAAGTGGCCA TCCCCGCCCCCGACTCCTGCGGCCGGTGCGGGAGCTGTCAGCTGCACGTTGCGGGGGTACCGGAGAAGCCGGGGTTGCCTTCTCCCGGTTCTGTGCGCTA ACCCCGGAGCCCGGCCCCGGGGAACTAGGGAGCGTCTCCGGCTGCACAGCGCTCAGGTTTTAGGGTTTCCTTTGGGCCACCCAAAATGTTTTTTAAATAC TTTGGATAAGAGCTAAACCACCTGCCACTGGGGACTTCCATACTCTCGGCTCCCTTGTCATCTGATGGCATTTTGATTGTCTCGAGGCCAAGTGACTTGC TGTCCATGATGAGTGATGAGAAGAACCTTGGTGTGTCCCAAAAATTGGTATCACCTTCAAGGAGCACAAGTAGCTGCTCTTCCAAGCAAGGAAGTCGACA GGACAGCTGGGAAGTGGTGGAAGGACTGAGGGGGGAGATGAATTACACCCAGGAGCCACCAGTTCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCC TTAAAAGGCTGGCATAAGAGATTCTTCTATCTGGACAAAGGAATCTTGAAATATGCCAAGAGCCAAACCGATATAGAGAGAGAGAAGCTGCATGGCTGCA TTGATGTCGGGCTCTCAGTGATGTCTGTAAAGAAGTCATCAAAATGCATAGACCTTGACACCGAGGAGCACATCTACCATCTGAAGGTCAAGTCAGAAGA AGTCTTTGATGAGTGGGTATCGAAACTTCGCCACCACAGAATGTATCGTCAGAATGAAATTGCCATGTTTCCACATGAAGTTAACCACTTTTTCTCAGGG TCCACCATCACAGACTCTTCATCTGGGGTGTTTGACTCCATTTCAAGTAGGAAGCGTAGCAGTATATCAAAGCAGAATTTATTTCAAACTGGAAGCAATG TATCATTTTCTTGTGGTGGTGAGACACGAGTTCCATTATGGTTACAGTCTTCAGAGGACATGGAAAAATGCTCCAAAGACCTGGCGCACTGTCATGCCTA CCTGGTAGAAATGAGCCAGCTCCTGCAAAGCATGGACGTCCTGCATCGGACATACTCGGCACCAGCTATCAACGCCATCCAGGTCCCGAAACCTTTTTCT GGCCCAGTAAGACTACACTCCTCCAATCCTAATTTGTCAACACTAGATTTTGGAGAAGAGAAAAATTATTCTGATGGCTCTGAAACCTCATCAGAGTTTT CTAAAATGCAAGAAGATCTGTGTCATATTGCCCATAAAGTTTACTTCACTTTAAGGTCAGCTTTTAATATCATGTCAGCGGAGAGAGAGAAACTGAAGCA GCTGATGGAGCAGGATGCCTCCTCCTCCCCGTCTGCTCAGGTCATTGGTCTGAAGAACGCCCTGTCATCCGCCCTAGCACAAAACACAGATCTTAAAGAA CGCTTACGCAGAATCCATGCCGAGTCTCTGCTCCTCGACTCCCCCGCTGTCGCCAAGTCGGGTGACAATCTGGCAGAGGAAAACTCCAGAGATGAAAACC GAGCTCTAGTTCATCAGCTTTCTAATGAAAGTAGACTCTCCATCACTGACTCCCTTTCTGAGTTTTTTGATGCTCAGGAAGTTCTGTTATCTCCAAGCTC TTCAGAAAACGAGATTTCTGATGATGACTCATATGTCAGTGACATAAGTGATAATCTTTCCTTAGATAATCTCAGTAATGATTTAGATAATGAGAGACAG ACCTTGGGGCCTGTCCTTGATAGTGGTCGGGAAGCGAAGTCCCGGAGAAGAACGTGCCTGCCGGCGCCCTGCCCGAGCAGCAGTAACATCAGCCTGTGGA ACATCCTGAGGAACAACATCGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGAGGCTCTGCGAGGAGCT GGAGTACAGCGAGCTCCTGGACAAGGCCGCGCAGATTCCCAGCCCCCTGGAAAGGATGGTATATGTGGCAGCCTTTGCCATATCAGCGTATGCATCTAGC TACTACCGAGCTGGAAGCAAGCCATTTAATCCGGTTCTTGGAGAAACATATGAATGTATTCGGGAGGACAAGGGCTTCCAGTTTTTTTCAGAACAGGTCA GCCACCATCCGCCTATCTCTGCGTGTCATGCTGAGTCTAGAAATTTTGTTTTCTGGCAAGATGTGAGATGGAAAAACAAATTCTGGGGCAAATCCATGGA AATTGTTCCAATTGGCACAACCCATGTGACTCTGCCAGTTTTTGGGGATCATTTTGAGTGGAACAAAGTGACCTCTTGCATCCATAACATCTTAAGCGGG CAGAGGTGGATTGAGCACTATGGAGAGATTGTCATCAAGAACCTGCATGATGATTCCTGCTACTGCAAAGTGAATTTTATAAAGGCAAAATACTGGAGCA CTAATGCCCATGAGATTGAAGGCACAGTGTTTGACAGGAGTGGAAAAGCGGTTCATCGGCTGTTTGGGAAATGGCATGAAAGCATCTACTGTGGCGGCGG CTCCTCTTCTGCCTGTGTATGGAGAGCAAATCCTATGCCGAAAGGCTACGAGCAATACTATAGCTTCACACAGTTTGCGCTGGAATTAAATGAAATGGAT CCATCATCAAAGTCTTTATTGCCACCTACTGACACTCGATTTAGGCCAGACCAGAGGTTTCTAGAGGAAGGGAACTTAGAAGAAGCTGAAATACAAAAGC AGAGGATTGAACAACTGCAGAGAGAAAGGCGGCGGGTCTTAGAAGAAAATCATGTGGAGCACCAGCCTCGGTTTTTCAGGAAATCCGACGATGACTCTTG GGTGAGCAACGGCACCTATTTGGAACTTAGAAAAGATCTTGGTTTTTCCAAACTGGACCATCCTGTCTTATGGTGAAAAAGTAAAGAAGAAAGATAACAT TAGTGTATTTCTCCTGTGCTTGCCTTCTGAAGTGGCACAAACCTGTGTTTATATATTTAAAAGATACTCTAGGATGATCACTTGTGCTTAGCTTAGCATT GTAACTCTTTAAGTCTATATTTTCCTCAGTGCGTTTCTTTACAATTTCAAATGTTACCCTGATTGTTTATATGAATGTAGAACACCTTGACATTTCTTTT TATATATAAACTATTTAATAAAAATGAAAGATTGAATGTTCATGTGTGGGTTAAAAAAAGAAGCTTTAACACTAATTTTCCAAAGGTTAGGGAAGATTCC AATTAAATTTATGCCTTATAAAATTATGTTGTAGAAAAAAAATCAACCTCTCCCAGGTGCATTAAGAAATAAGAATTCCCAGGGTTACTCACCCATGCGT AAGCTACCCAAGTTTAATTTGGTAGCTGAAATATCTTTTTGCCTCAGACAGCTCTTGAATTGCTCATACAGAACAATTCTGCTGGTGCTGGAGTCTGAAG AATATTTTCATTTGCATTTTAGTGGTTAGGGAGAGGATATAAGATTAATGGAATGTATATTTTTATATAAGACGTATACGGCACCTTCTTGAAAAGGAAG CATTTGAACTTGTTCCTCCCTATAGTTCTATTGCCTTATATGCAAAATTGTACCCTGTTGCTCAGAGAAATTATTCATAAGAGAAAAGAATTCCAATTAA TTAAATATCACAATAGCATCCCAGAGAGACAGTAGGAAATTTCTCCTTAGTGAGAGCTGAGTCCTTGAGAAGTTAAGAGACTGTTTCCTGCTTCCCACCC CACCCCGTTTTTTGTCCCTCCGTTTGATCACCTCCCTGTTGAGTATGGAATGTCCCAGTTTAATATAAAACATACAGTTTATTCCACACAGCAGTTTGAC TTTGTAAAAGTTTAGCAAAATAACTGATTGTTTTGGATAACTCAACATGTTTTTCTTAAATGCTGTTTTAGTATTTTCTACCTCTTAATAAGCACCATAT TTTAGATCTTGTATAAAAAGTTGCCATCTGCCTTATAGACAAATGTAGAGAATTGATGTTCTTGCTTTGTGTTGTGTTCTGGTAAAGCTTTAAGTGAGTG TCTTACCCCTTTCCTATACTTTCTTGTTATCCATATCATTCTTTTGTGAGTTTTGCAGCAGTCTTGAATAATACAGATTATAACTACTGAAAGGGGAACC TTCGTCTTTTTAAATGTGTTATTTCACATTACAATAATATGTGTAGTAGTTGAATTGTGTTATCAAAGCATGTCCAACTTCTGTCCAGATGACATTAAAA CCAAAGCCTAATTGTTAAGCCTCTTATTCTAAAGTCCATAGCAATGCTATGGAATATCAAGTATAATGATGTAGTAAAAAGATTTCTCCAGAAAACACTT TGAGATACTTAAAGAAATTCAGAGCATATCGAATGTTATGAATGAAGAGTAAAATAATTACAGGAAAAGAATGCATGATTTTTCATTGTCACACCAAATA ACCAGTTGGACAATATTGCATTTTCAAAATGGAGAGTTATTTAAAGTGGATGTGTAGCCTTCTGGAATCTGCACGTGACTGGCATTTTAAAACAACTCAT TAAATATTGGATTCATTTTATCGAGATGCCAAGATGAACACAATGTGAATATCATTTCCATTTCTAAGCAGTTGAATGACACAGTCAGATTCTTTGGTGT ATGCTTTGTTCTTTCCATCTCAAGCATTTATCCAAATTCTGCTCACAGACATGAGGAAGCAGGCTGTAGATTTAAGGGCACTCAAGGGGAAAACAAATGT AGTTTCCACAGTGTGAGGATAAATGTAGAATCGTGGTAATATTGGCTGTTAAATTGGCCTGCTCCAGAGACTGGCTCAAAAAAGAAAGAAGCAACGAAAA CAAAATGCACAGCCTGAATGGACGTAGCACTGTCATGTGGCATTTGGAAAATCTTTAATTATCCTAATGTTATTTCACTTGCCCTTATGCTTTATTTTAG AGTCCCAGGGACAACTGGATGAAACATTACGTCTGTAGTCATCTTATTTGAGATGGGGGTGGGAACGAATTAAAATATGATAGAGAACTATATGCAGATT TTAAAATCTAGTCAGTACTAGCATCTATAAAGGGCTTTTCTGAAATTTAAACAGCTTGGTGATGCATCCTGATATTATAAGCTTAAAACTATCTTGAGGG GGAAATGACTTCTTTTCTCCTTCTGTCCCTTTTCTCAAAAGCATCTTTCCCCCAAATGTATGTCTCTAGGACATTTTATAGTTTTAAGAGGAAGAAAACA GAAACGTGGCATCATTTGGGAAGAACCAAGGTAGAATTTAATTTTCTGGACTTTAAATCTCCTGGTTAAATAATGCTAATCACTGTACCATTTGAGTTAC ATGTTTTAACCTCTTTTTTTTTTCTAATTATTCCATCCAAAATTTCTGGTGAATTACACAGAAATTTTTGGAAATGGGACTTTGTGCAGGTAACCACCCT GCACTTCAGGTAATGTTCCTGTCACAGCTGTCGACTACCCCACGCTGCAGGAAATCCATTCAGAAATGAGCTAACAGTCTCCAGTGTAGCAGGAGAGCCA GTCACTTCCCTCCCCAGGCCCCAAAGCACCCCAAGGCTGGTATTCCTTGAGTCATAGGTATTAATAATAAAAGCCTCAATGCAGCTTCTCCATGTAGTTC CTCTCCTACAAGCCAGGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACCTGAGGGCAAGAAGTACCCAGGATGCCCAAACAGCCGCACAGGTGTC CTGTGCTTCCTGTGAGACTTCCTGGGGGAAATATACAAACTAATATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCATATTCTGCTTTTA ATTACTTGTAGTAAATCATTTGTTTGGGCTTCAAGCACTGTCTTCCATCTGCATCTTCCAGATTTATCATGAGTGATCTTGTTCTACTGTTAAAGATGTC AAGCCTAAAAAGTAATAAAGTTTTTATTTGGCTGTTGAACCTTGATGTAGCCCCTACTACATACACTACAAGTTATGCCTTCTGGCTACTACAGAATCTC CGCAGACCTTTTAGTTATGAGTAGAAGCAATAAGAAGGTGTCCTTATGGTCTTAGGAATAATAGTTCCAATGTATTGGCATAATTTGTTTGAATGTCTTT ATTTTGATATTAACACCGGCATAAATCTATTTTTGCTACCAGATGATAGCTATTTTCATTTGCTCTATTTTCCTCTGCATTGTACTAATCTGTTACTCAG TGTTAGTCTTCATTTTTCTGTGTTGGAAACCTGCGTCATGTAAATATCTGCAAATCATGACAGTCTAAAGTGCAAACCATTTTCAGAGACTTTGGGGAAT GCAGTGATCAGAAGTGGCTAATTTATTTTATTTCTGATTATTTTATCCAGAGGGTACTTTTTTAATGGATATTTGTAAATCTTCCACTTAACCACTGAAA ATTATTTTGTTTTAATCCCACCCTTCCATCCATACCTCTGCCTCCCCAAAAAGCTCCTATTAATTTGCTTATCCCCCTCATGTAGCTAGTTGAATGTGAA TAAATAACATGGAAAAAAA

refseq_OSBPL3.F2 (forward primer)
refseq_OSBPL3.R2 (reverse primer)
Single exon skipping

Frame 0

AGGARQPARERGLERFAAGAQRGSGPAVCLQVA IPAPDSCGRCGSCQLHVAGVPEKPGLPSPGSVR* PRSPAPGN*GASPAAQRSGFRVSFGPPKMFFKY FG*ELNHLPLGTSILSAPLSSDGILIVSRPSDL LSMMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQ DSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWP LKGWHKRFFYLDKGILKYAKSQTDIEREKLHGC IDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEE VFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSG STITDSSSGVFDSISSRKRSSISKQNLFQTGSN VSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAY LVEMSQLLQSMDVLHRTYSAPAINAIQVPKPFS GPVRLHSSNPNLSTLDFGEEKNYSDGSETSSEF SKMQEDLCHIAHKVYFTLRSAFNIMSAEREKLKQ LMEQDASSSPSAQVIGLKNALSSALAQNTDLKE RLRRIHAESLLLDSPAVAKSGDNLAEENSRDEN RALVHQLSNESRLSITDSLSEFFDAQEVLLSPSS SENEISDDDSYVSDISDNLSLDNLSNDLDNERQ TLGPVLDSGREAKSRRRTCLPAPCPSSSNISLW NILRNNIGKDLSKVAMPVELNEPLNTLQRLCEEL EYSELLDKAAQIPSPLERMVYVAAFAISAYASS YYRAGSKPFNPVLGETYECIREDKGFQFFSEQV SHHPPISACHAESRNFVFWQDVRWKNKFWGKSME IVPIGTTHVTLPVFGDHFEWNKVTSCIHNILSG QRWIEHYGEIVIKNLHDDSCYCKVNFIKAKYWS TNAHEIEGTVFDRSGKAVHRLFGKWHESIYCGGG SSSACVWRANPMPKGYEQYYSFTQFALELNEMD PSSKSLLPPTDTRFRPDQRFLEEGNLEEAEIQK QRIEQLQRERRRVLEENHVEHQPRFFRKSDDDSW VSNGTYLELRKDLGFSKLDHPVLW*KSKEER*H *CISPVLAF*SGTNLCLYI*KIL*DDHLCLA*H CNSLSLYFPQCVSLQFQMLP*LFI*M*NTLTFLF IYKLFNKNERLNVHVWVKKRSFNTNFPKVREDS N*IYAL*NYVVEKKSTSPRCIKK*EFPGLLTHA *ATQV*FGS*NIFLPQTALELLIQNNSAGAGV*R IFSFAF*WLGRGYKINGMYIFI*DVYGTFLKRK HLNLFLPIVLLPYMQNCTLLLREIIHKRKEFQL IKYHNSIPERQ*EISP**ELSP*EVKRLFPASHP TPFFVPPFDHLPVEYGMSQFNIKHTVYSTQQFD FVKV*QNN*LFWITQHVFLKCCFSIFYLLISTI F*ILYKKLPSAL*TNVEN*CSCFVLCSGKALSEC LTPFLYFLVIHIILL*VLQQS*IIQIITTERGT FVFLNVLFHITIICVVVELCYQSMSNFCPDDIK TKA*LLSLLF*SP*QCYGISSIMM**KDFSRKHF EILKEIQSISNVMNEE*NNYRKRMHDFSLSHQI TSWTILHFQNGELFKVDV*PSGICT*LAF*NNS LNIGFILSRCQDEHNVNIISISKQLNDTVRFFGV CFVLSISSIYPNSAHRHEEAGCRFKGTQGENKC SFHSVRINVESW*YWLLNWPAPETGSKKKEATK TKCTA*MDVALSCGIWKIFNYPNVISLALMLYFR VPGTTG*NITSVVILFEMGVGTN*NMIENYMQI LKSSQY*HL*RAFLKFKQLGDAS*YYKLKTILR GK*LLFSFCPFSQKHLSPKCMSLGHFIVLRGRKQ KRGIIWEEPR*NLIFWTLNLLVK*C*SLYHLSY MF*PLFFF*LFHPKFLVNYTEIFGNGTLCR*PP CTSGNVPVTAVDYPTLQEIHSEMS*QSPV*QESQ SLPSPGPKAPQGWYSLSHRY***KPQCSFSM*F LSYKPGGFWS*LKRWEFT*GQEVPRMPKQPHRC PVLPVRLPGGNIQTNIFFNVYVIYTAAFLIFCF* LLVVNHLFGLQALSSICIFQIYHE*SCSTVKDV KPKK**SFYLAVEP*CSPYYIHYKLCLLATTES PQTF*L*VEAIRRCPYGLRNNSSNVLA*FV*MSL F*Y*HRHKSIFATR**LFSFALFSSALY*SVTQ C*SSFFCVGNLRHVNICKS*QSKVQTIFRDFGE CSDQKWLIYFISDYFIQRVLF*WIFVNLPLNH*K LFCFNPTLPSIPLPPQKAPINLLIPLM*LVECE *ITWKK

Frame 1

LVAPASRPGRGDLSASRPVRSAAPAPQSVCKWP SPPPTPAAGAGAVSCTLRGYRRSRGCLLPVLCA NPGARPRGTRERLRLHSAQVLGFPLGHPKCFLNT LDKS*TTCHWGLPYSRLPCHLMAF*LSRGQVTC CP**VMRRTLVCPKNWYHLQGAQVAALPSKEVD RTAGKWWKD*GGR*ITPRSHQFRKDFC*KRGSGP *KAGIRDSSIWTKES*NMPRAKPI*RERSCMAA LMSGSQ*CL*RSHQNA*TLTPRSTSTI*RSSQK KSLMSGYRNFATTECIVRMKLPCFHMKLTTFSQG PPSQTLHLGCLTPFQVGSVAVYQSRIYFKLEAM YHFLVVVRHEFHYGYSLQRTWKNAPKTWRTVMP TW*K*ASSCKAWTSCIGHTRHQLSTPSRSRNLFL AQ*DYTPPILICQH*ILEKRKIILMALKPHQSF LKCKKICVILPIKFTSL*GQLLISCQRRERN*S S*WSRMPPPPRLLRSLV*RTPCHPP*HKTQILKN AYAESMPSLCSSTPPLSPSRVTIWQRKTPEMKT EL*FISFLMKVDSPSLTPFLSFLMLRKFCYLQA LQKTRFLMMTHMSVT*VIIFP*IISVMI*IMRDR PWGLSLIVVGKRSPGEERACRRPARAAVTSACG TS*GTTSGRTCPRWPCRWS*TSP*TRCRGSARS WSTASSWTRPRRFPAPWKGWYMWQPLPYQRMHLA TTELEASHLIRFLEKHMNVFGRTRASSFFQNRS ATIRLSLRVMLSLEILFSGKM*DGKTNSGANPW KLFQLAQPM*LCQFLGIILSGTK*PLASITS*AG RGGLSTMERLSSRTCMMIPATAK*IL*RQNTGA LMPMRLKAQCLTGVEKRFIGCLGNGMKASTVAA APLLPVYGEQILCRKATSNTIASHSLRWN*MKWI HHQSLYCHLLTLDLGQTRGF*RKGT*KKLKYKS RGLNNCREKGGGS*KKIMWSTSLGFSGNPTMTL G*ATAPIWNLEKILVFPNWTILSYGEKVKKKDNI SVFLLCLPSEVAQTCVYIFKRYSRMITCA*LSI VTL*VYIFLSAFLYNFKCYPDCLYECRTP*HFF LYINYLIKMKD*MFMCGLKKEALTLIFQRLGKIP IKFMPYKIML*KKNQPLPGALRNKNSQGYSPMR KLPKFNLVAEISFCLRQLLNCSYRTILLVLESE EYFHLHFSG*GEDIRLMECIFLYKTYTAPS*KGS I*TCSSL*FYCLICKIVPCCSEKLFIREKNSN* LNITIASQRDSRKFLLSES*VLEKLRDCFLLPT PPRFLSLRLITSLLSMECPSLI*NIQFIPHSSLT L*KFSKITDCFG*LNMFFLNAVLVFSTS**APY FRSCIKSCHLPYRQM*RIDVLALCCVLVKL*VS VLPLSYTFLLSISFFCEFCSSLE*YRL*LLKGEP SSF*MCYFTLQ*YV**LNCVIKACPTSVQMTLK PKPNC*ASYSKVHSNAMEYQV**CSKKISPENT LRYLKKFRAYRML*MKSKIITGKECMIFHCHTK* PVGQYCIFKMESYLKWMCSLLESARDWHFKTTH *ILDSFYRDAKMNTM*ISFPFLSS*MTQSDSLV YALFFPSQAFIQILLTDMRKQAVDLRALKGKTNV VSTV*G*M*NRGNIGC*IGLLQRLAQKRKKQRK QNAQPEWT*HCHVAFGKSLIILMLFHLPLCFIL ESQGQLDETLRL*SSYLRWGWERIKI**RTICRF *NLVSTSIYKGLF*NLNSLVMHPDIISLKLS*G GNDFFSPSVPFLKSIFPPNVCL*DIL*F*EEEN RNVASFGKNQGRI*FSGL*ISWLNNANHCTI*VT CFNLFFFSNYSIQNFW*ITQKFLEMGLCAGNHP ALQVMFLSQLSTTPRCRKSIQK*ANSLQCSRRA SHFPPQAPKHPKAGIP*VIGINNKSLNAASPCSS SPTSQVDSGPN*RDGSSPEGKKYPGCPNSRTGV LCFL*DFLGEIYKLIYFLMFTSFTLLLFSYSAF NYL**IICLGFKHCLPSASSRFIMSDLVLLLKMS SLKSNKVFIWLLNLDVAPTTYTTSYAFWLLQNL RRPFSYE*KQ*EGVLMVLGIIVPMYWHNLFECL YFDINTGINLFLLPDDSYFHLLYFPLHCTNLLLS VSLHFSVLETCVM*ISANHDSLKCKPFSETLGN AVIRSG*FILFLIILSRGYFFNGYL*IFHLTTE NYFVLIPPFHPYLCLPKKLLLICLSPSCS*LNVN K*HGKK

Frame 2

WWRPPAGPGEGT*ALRGRCAARLRPRSLSASGH PRPRLLRPVRELSAARCGGTGEAGVAFSRFCAL TPEPGPGELGSVSGCTALRF*GFLWATQNVF*I LWIRAKPPATGDFHTLGSLVI*WHFDCLEAK*LA VHDE**EEPWCVPKIGITFKEHK*LLFQARKST GQLGSGGRTEGGDELHPGATSSERIFAEKEEVA LKRLA*EILLSGQRNLEICQEPNRYREREAAWLH *CRALSDVCKEVIKMHRP*HRGAHLPSEGQVRR SL**VGIETSPPQNVSSE*NCHVST*S*PLFLR VHHHRLFIWGV*LHFK*EA*QYIKAEFISNWKQC IIFLWW*DTSSIMVTVFRGHGKMLQRPGALSCL PGRNEPAPAKHGRPASDILGTSYQRHPGPETFF WPSKTTLLQS*FVNTRFWRREKLF*WL*NLIRVF *NARRSVSYCP*SLLHFKVSF*YHVSGERETEA ADGAGCLLLPVCSGHWSEERPVIRPSTKHRS*R TLTQNPCRVSAPRLPRCRQVG*QSGRGKLQR*KP SSSSSAF**K*TLHH*LPF*VF*CSGSSVISKL FRKRDF***LICQ*HK**SFLR*SQ**FR**ET DLGACP**WSGSEVPEKNVPAGALPEQQ*HQPVE HPEEQHREGPVQGGHAGGAERAPEHAAEALRGA GVQRAPGQGRADSQPPGKDGICGSLCHISVCI* LLPSWKQAI*SGSWRNI*MYSGGQGLPVFFRTGQ PPSAYLCVSC*V*KFCFLARCEMEKQILGQIHG NCSNWHNPCDSASFWGSF*VEQSDLLHP*HLKR AEVD*ALWRDCHQEPA**FLLLQSEFYKGKILEH *CP*D*RHSV*QEWKSGSSAVWEMA*KHLLWRR LLFCLCMESKSYAERLRAIL*LHTVCAGIK*NG SIIKVFIATY*HSI*ARPEVSRGRELRRS*NTKA ED*TTAERKAAGLRRKSCGAPASVFQEIRR*LL GEQRHLFGT*KRSWFFQTGPSCLMVKK*RRKIT LVYFSCACLLKWHKPVFIYLKDTLG*SLVLSLAL *LFKSIFSSVRFFTISNVTLIVYMNVEHLDISF YI*TI**K*KIECSCVG*KKKL*H*FSKG*GRF QLNLCLIKLCCRKKINLSQVH*EIRIPRVTHPCV SYPSLIW*LKYLFASDSS*IAHTEQFCWCWSLK NIFICILVVRERI*D*WNVYFYIRRIRHLLEKE AFELVPPYSSIALYAKLYPVAQRNYS*EKRIPIN *ISQ*HPRETVGNFSLVRAESLRS*ETVSCFPP HPVFCPSV*SPPC*VWNVPV*YKTYSLFHTAV* LCKSLAK*LIVLDNSTCFS*MLF*YFLPLNKHHI LDLV*KVAICLIDKCRELMFLLCVVFW*SFK*V SYPFPILSCYPYHSFVSFAAVLNNTDYNY*KGN LRLFKCVISHYNNMCSS*IVLSKHVQLLSR*H*N QSLIVKPLILKSIAMLWNIKYNDVVKRFLQKTL *DT*RNSEHIECYE*RVK*LQEKNA*FFIVTPN NQLDNIAFSKWRVI*SGCVAFWNLHVTGILKQLI KYWIHFIEMPR*TQCEYHFHF*AVE*HSQILWC MLCSFHLKHLSKFCSQT*GSRL*I*GHSRGKQM *FPQCEDKCRIVVILAVKLACSRDWLKKERSNEN KMHSLNGRSTVMWHLENL*LS*CYFTCPYALF* SPRDNWMKHYVCSHLI*DGGGNELKYDRELYAD FKI*SVLASIKGFSEI*TAW*CILIL*A*NYLEG EMTSFLLLSLFSKASFPQMYVSRTFYSFKRKKT ETWHHLGRTKVEFNFLDFKSPG*IMLITVPFEL HVLTSFFFLIIPSKISGELHRNFWKWDFVQVTTL HFR*CSCHSCRLPHAAGNPFRNELTVSSVAGEP VTSLPRPQSTPRLVFLES*VLIIKASMQLLHVV PLLQARWILVLTKEMGVHLRARSTQDAQTAAQVS CASCETSWGKYTN*YIF*CLRHLHCCFSHILLL ITCSKSFVWASSTVFHLHLPDLS*VILFYC*RC QA*KVIKFLFGC*TLM*PLLHTLQVMPSGYYRIS ADLLVMSRSNKKVSLWS*E**FQCIGIICLNVF ILILTPA*IYFCYQMIAIFICSIFLCIVLICYS VLVFIFLCWKPASCKYLQIMTV*SANHFQRLWGM Q*SEVANLFYF*LFYPEGTFLMDICKSST*PLK IILF*SHPSIHTSASPKSSY*FAYPPHVAS*M* INNMEK

Sequence NM_145322

GCTGGTGGCGCCCGCCAGCCGGCCCGGGAGAGGGGACTTGAGCGCTTCGCGGCCGGTGCGCAGCGCGGCTCCGGCCCCGCAGTCTGTCTGCAAGTGGCCA TCCCCGCCCCCGACTCCTGCGGCCGGTGCGGGAGCTGTCAGCTGCACGTTGCGGGGGTACCGGAGAAGCCGGGGTTGCCTTCTCCCGGTTCTGTGCGCTA ACCCCGGAGCCCGGCCCCGGGGAACTAGGGAGCGTCTCCGGCTGCACAGCGCTCAGGTTTTAGGGTTTCCTTTGGGCCACCCAAAATGTTTTTTAAATAC TTTGGATAAGAGCTAAACCACCTGCCACTGGGGACTTCCATACTCTCGGCTCCCTTGTCATCTGATGGCATTTTGATTGTCTCGAGGCCAAGTGACTTGC TGTCCATGATGAGTGATGAGAAGAACCTTGGTGTGTCCCAAAAATTGGTATCACCTTCAAGGAGCACAAGTAGCTGCTCTTCCAAGCAAGGAAGTCGACA GGACAGCTGGGAAGTGGTGGAAGGACTGAGGGGGGAGATGAATTACACCCAGGAGCCACCAGTTCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCC TTAAAAGGCTGGCATAAGAGATTCTTCTATCTGGACAAAGGAATCTTGAAATATGCCAAGAGCCAAACCGATATAGAGAGAGAGAAGCTGCATGGCTGCA TTGATGTCGGGCTCTCAGTGATGTCTGTAAAGAAGTCATCAAAATGCATAGACCTTGACACCGAGGAGCACATCTACCATCTGAAGGTCAAGTCAGAAGA AGTCTTTGATGAGTGGGTATCGAAACTTCGCCACCACAGAATGTATCGTCAGAATGAAATTGCCATGTTTCCACATGAAGTTAACCACTTTTTCTCAGGG TCCACCATCACAGACTCTTCATCTGGGGTGTTTGACTCCATTTCAAGTAGGAAGCGTAGCAGTATATCAAAGCAGAATTTATTTCAAACTGGAAGCAATG TATCATTTTCTTGTGGTGGTGAGACACGAGTTCCATTATGGTTACAGTCTTCAGAGGACATGGAAAAATGCTCCAAAGACCTGGCGCACTGTCATGCCTA CCTGGTAGAAATGAGCCAGCTCCTGCAAAGCATGGACGTCCTGCATCGGACATACTCGGCACCAGCTATCAACGCCATCCAGGTCCCGAAACCTTTTTCT GGCCCAGTAAGACTACACTCCTCCAATCCTAATTTGTCAACACTAGATTTTGGAGAAGAGAAAAATTATTCTGATGGCTCTGAAACCTCATCAGAGTTTT CTAAAATGCAAGAAGATCTGTGTCATATTGCCCATAAAGTTTACTTCACTTTAAGGTCAGCTTTTAATATCATGTCAGCGGAGAGAGAGAAACTGAAGCA GCTGATGGAGCAGGATGCCTCCTCCTCCCCGTCTGCTCAGGTCATTGGTCTGAAGAACGCCCTGTCATCCGAAAACTCCAGAGATGAAAACCGAGCTCTA GTTCATCAGCTTTCTAATGAAAGTAGACTCTCCATCACTGACTCCCTTTCTGAGTTTTTTGATGCTCAGGAAGTTCTGTTATCTCCAAGCTCTTCAGAAA ACGAGATTTCTGATGATGACTCATATGTCAGTGACATAAGTGATAATCTTTCCTTAGATAATCTCAGTAATGATTTAGATAATGAGAGACAGACCTTGGG GCCTGTCCTTGATAGTGGTCGGGAAGCGAAGTCCCGGAGAAGAACGTGCCTGCCGGCGCCCTGCCCGAGCAGCAGTAACATCAGCCTGTGGAACATCCTG AGGAACAACATCGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGAGGCTCTGCGAGGAGCTGGAGTACA GCGAGCTCCTGGACAAGGCCGCGCAGATTCCCAGCCCCCTGGAAAGGATGGTATATGTGGCAGCCTTTGCCATATCAGCGTATGCATCTAGCTACTACCG AGCTGGAAGCAAGCCATTTAATCCGGTTCTTGGAGAAACATATGAATGTATTCGGGAGGACAAGGGCTTCCAGTTTTTTTCAGAACAGGTCAGCCACCAT CCGCCTATCTCTGCGTGTCATGCTGAGTCTAGAAATTTTGTTTTCTGGCAAGATGTGAGATGGAAAAACAAATTCTGGGGCAAATCCATGGAAATTGTTC CAATTGGCACAACCCATGTGACTCTGCCAGTTTTTGGGGATCATTTTGAGTGGAACAAAGTGACCTCTTGCATCCATAACATCTTAAGCGGGCAGAGGTG GATTGAGCACTATGGAGAGATTGTCATCAAGAACCTGCATGATGATTCCTGCTACTGCAAAGTGAATTTTATAAAGGCAAAATACTGGAGCACTAATGCC CATGAGATTGAAGGCACAGTGTTTGACAGGAGTGGAAAAGCGGTTCATCGGCTGTTTGGGAAATGGCATGAAAGCATCTACTGTGGCGGCGGCTCCTCTT CTGCCTGTGTATGGAGAGCAAATCCTATGCCGAAAGGCTACGAGCAATACTATAGCTTCACACAGTTTGCGCTGGAATTAAATGAAATGGATCCATCATC AAAGTCTTTATTGCCACCTACTGACACTCGATTTAGGCCAGACCAGAGGTTTCTAGAGGAAGGGAACTTAGAAGAAGCTGAAATACAAAAGCAGAGGATT GAACAACTGCAGAGAGAAAGGCGGCGGGTCTTAGAAGAAAATCATGTGGAGCACCAGCCTCGGTTTTTCAGGAAATCCGACGATGACTCTTGGGTGAGCA ACGGCACCTATTTGGAACTTAGAAAAGATCTTGGTTTTTCCAAACTGGACCATCCTGTCTTATGGTGAAAAAGTAAAGAAGAAAGATAACATTAGTGTAT TTCTCCTGTGCTTGCCTTCTGAAGTGGCACAAACCTGTGTTTATATATTTAAAAGATACTCTAGGATGATCACTTGTGCTTAGCTTAGCATTGTAACTCT TTAAGTCTATATTTTCCTCAGTGCGTTTCTTTACAATTTCAAATGTTACCCTGATTGTTTATATGAATGTAGAACACCTTGACATTTCTTTTTATATATA AACTATTTAATAAAAATGAAAGATTGAATGTTCATGTGTGGGTTAAAAAAAGAAGCTTTAACACTAATTTTCCAAAGGTTAGGGAAGATTCCAATTAAAT TTATGCCTTATAAAATTATGTTGTAGAAAAAAAATCAACCTCTCCCAGGTGCATTAAGAAATAAGAATTCCCAGGGTTACTCACCCATGCGTAAGCTACC CAAGTTTAATTTGGTAGCTGAAATATCTTTTTGCCTCAGACAGCTCTTGAATTGCTCATACAGAACAATTCTGCTGGTGCTGGAGTCTGAAGAATATTTT CATTTGCATTTTAGTGGTTAGGGAGAGGATATAAGATTAATGGAATGTATATTTTTATATAAGACGTATACGGCACCTTCTTGAAAAGGAAGCATTTGAA CTTGTTCCTCCCTATAGTTCTATTGCCTTATATGCAAAATTGTACCCTGTTGCTCAGAGAAATTATTCATAAGAGAAAAGAATTCCAATTAATTAAATAT CACAATAGCATCCCAGAGAGACAGTAGGAAATTTCTCCTTAGTGAGAGCTGAGTCCTTGAGAAGTTAAGAGACTGTTTCCTGCTTCCCACCCCACCCCGT TTTTTGTCCCTCCGTTTGATCACCTCCCTGTTGAGTATGGAATGTCCCAGTTTAATATAAAACATACAGTTTATTCCACACAGCAGTTTGACTTTGTAAA AGTTTAGCAAAATAACTGATTGTTTTGGATAACTCAACATGTTTTTCTTAAATGCTGTTTTAGTATTTTCTACCTCTTAATAAGCACCATATTTTAGATC TTGTATAAAAAGTTGCCATCTGCCTTATAGACAAATGTAGAGAATTGATGTTCTTGCTTTGTGTTGTGTTCTGGTAAAGCTTTAAGTGAGTGTCTTACCC CTTTCCTATACTTTCTTGTTATCCATATCATTCTTTTGTGAGTTTTGCAGCAGTCTTGAATAATACAGATTATAACTACTGAAAGGGGAACCTTCGTCTT TTTAAATGTGTTATTTCACATTACAATAATATGTGTAGTAGTTGAATTGTGTTATCAAAGCATGTCCAACTTCTGTCCAGATGACATTAAAACCAAAGCC TAATTGTTAAGCCTCTTATTCTAAAGTCCATAGCAATGCTATGGAATATCAAGTATAATGATGTAGTAAAAAGATTTCTCCAGAAAACACTTTGAGATAC TTAAAGAAATTCAGAGCATATCGAATGTTATGAATGAAGAGTAAAATAATTACAGGAAAAGAATGCATGATTTTTCATTGTCACACCAAATAACCAGTTG GACAATATTGCATTTTCAAAATGGAGAGTTATTTAAAGTGGATGTGTAGCCTTCTGGAATCTGCACGTGACTGGCATTTTAAAACAACTCATTAAATATT GGATTCATTTTATCGAGATGCCAAGATGAACACAATGTGAATATCATTTCCATTTCTAAGCAGTTGAATGACACAGTCAGATTCTTTGGTGTATGCTTTG TTCTTTCCATCTCAAGCATTTATCCAAATTCTGCTCACAGACATGAGGAAGCAGGCTGTAGATTTAAGGGCACTCAAGGGGAAAACAAATGTAGTTTCCA CAGTGTGAGGATAAATGTAGAATCGTGGTAATATTGGCTGTTAAATTGGCCTGCTCCAGAGACTGGCTCAAAAAAGAAAGAAGCAACGAAAACAAAATGC ACAGCCTGAATGGACGTAGCACTGTCATGTGGCATTTGGAAAATCTTTAATTATCCTAATGTTATTTCACTTGCCCTTATGCTTTATTTTAGAGTCCCAG GGACAACTGGATGAAACATTACGTCTGTAGTCATCTTATTTGAGATGGGGGTGGGAACGAATTAAAATATGATAGAGAACTATATGCAGATTTTAAAATC TAGTCAGTACTAGCATCTATAAAGGGCTTTTCTGAAATTTAAACAGCTTGGTGATGCATCCTGATATTATAAGCTTAAAACTATCTTGAGGGGGAAATGA CTTCTTTTCTCCTTCTGTCCCTTTTCTCAAAAGCATCTTTCCCCCAAATGTATGTCTCTAGGACATTTTATAGTTTTAAGAGGAAGAAAACAGAAACGTG GCATCATTTGGGAAGAACCAAGGTAGAATTTAATTTTCTGGACTTTAAATCTCCTGGTTAAATAATGCTAATCACTGTACCATTTGAGTTACATGTTTTA ACCTCTTTTTTTTTTCTAATTATTCCATCCAAAATTTCTGGTGAATTACACAGAAATTTTTGGAAATGGGACTTTGTGCAGGTAACCACCCTGCACTTCA GGTAATGTTCCTGTCACAGCTGTCGACTACCCCACGCTGCAGGAAATCCATTCAGAAATGAGCTAACAGTCTCCAGTGTAGCAGGAGAGCCAGTCACTTC CCTCCCCAGGCCCCAAAGCACCCCAAGGCTGGTATTCCTTGAGTCATAGGTATTAATAATAAAAGCCTCAATGCAGCTTCTCCATGTAGTTCCTCTCCTA CAAGCCAGGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACCTGAGGGCAAGAAGTACCCAGGATGCCCAAACAGCCGCACAGGTGTCCTGTGCTT CCTGTGAGACTTCCTGGGGGAAATATACAAACTAATATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCATATTCTGCTTTTAATTACTTG TAGTAAATCATTTGTTTGGGCTTCAAGCACTGTCTTCCATCTGCATCTTCCAGATTTATCATGAGTGATCTTGTTCTACTGTTAAAGATGTCAAGCCTAA AAAGTAATAAAGTTTTTATTTGGCTGTTGAACCTTGATGTAGCCCCTACTACATACACTACAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGACC TTTTAGTTATGAGTAGAAGCAATAAGAAGGTGTCCTTATGGTCTTAGGAATAATAGTTCCAATGTATTGGCATAATTTGTTTGAATGTCTTTATTTTGAT ATTAACACCGGCATAAATCTATTTTTGCTACCAGATGATAGCTATTTTCATTTGCTCTATTTTCCTCTGCATTGTACTAATCTGTTACTCAGTGTTAGTC TTCATTTTTCTGTGTTGGAAACCTGCGTCATGTAAATATCTGCAAATCATGACAGTCTAAAGTGCAAACCATTTTCAGAGACTTTGGGGAATGCAGTGAT CAGAAGTGGCTAATTTATTTTATTTCTGATTATTTTATCCAGAGGGTACTTTTTTAATGGATATTTGTAAATCTTCCACTTAACCACTGAAAATTATTTT GTTTTAATCCCACCCTTCCATCCATACCTCTGCCTCCCCAAAAAGCTCCTATTAATTTGCTTATCCCCCTCATGTAGCTAGTTGAATGTGAATAAATAAC ATGGAAAAAAA

refseq_OSBPL3.F2 (forward primer)
refseq_OSBPL3.R2 (reverse primer)
Single exon skipping

Frame 0

AGGARQPARERGLERFAAGAQRGSGPAVCLQVA IPAPDSCGRCGSCQLHVAGVPEKPGLPSPGSVR* PRSPAPGN*GASPAAQRSGFRVSFGPPKMFFKY FG*ELNHLPLGTSILSAPLSSDGILIVSRPSDL LSMMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQ DSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWP LKGWHKRFFYLDKGILKYAKSQTDIEREKLHGC IDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEE VFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSG STITDSSSGVFDSISSRKRSSISKQNLFQTGSN VSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAY LVEMSQLLQSMDVLHRTYSAPAINAIQVPKPFS GPVRLHSSNPNLSTLDFGEEKNYSDGSETSSEF SKMQEDLCHIAHKVYFTLRSAFNIMSAEREKLKQ LMEQDASSSPSAQVIGLKNALSSENSRDENRAL VHQLSNESRLSITDSLSEFFDAQEVLLSPSSSE NEISDDDSYVSDISDNLSLDNLSNDLDNERQTLG PVLDSGREAKSRRRTCLPAPCPSSSNISLWNIL RNNIGKDLSKVAMPVELNEPLNTLQRLCEELEY SELLDKAAQIPSPLERMVYVAAFAISAYASSYYR AGSKPFNPVLGETYECIREDKGFQFFSEQVSHH PPISACHAESRNFVFWQDVRWKNKFWGKSMEIV PIGTTHVTLPVFGDHFEWNKVTSCIHNILSGQRW IEHYGEIVIKNLHDDSCYCKVNFIKAKYWSTNA HEIEGTVFDRSGKAVHRLFGKWHESIYCGGGSS SACVWRANPMPKGYEQYYSFTQFALELNEMDPSS KSLLPPTDTRFRPDQRFLEEGNLEEAEIQKQRI EQLQRERRRVLEENHVEHQPRFFRKSDDDSWVS NGTYLELRKDLGFSKLDHPVLW*KSKEER*H*CI SPVLAF*SGTNLCLYI*KIL*DDHLCLA*HCNS LSLYFPQCVSLQFQMLP*LFI*M*NTLTFLFIY KLFNKNERLNVHVWVKKRSFNTNFPKVREDSN*I YAL*NYVVEKKSTSPRCIKK*EFPGLLTHA*AT QV*FGS*NIFLPQTALELLIQNNSAGAGV*RIF SFAF*WLGRGYKINGMYIFI*DVYGTFLKRKHLN LFLPIVLLPYMQNCTLLLREIIHKRKEFQLIKY HNSIPERQ*EISP**ELSP*EVKRLFPASHPTP FFVPPFDHLPVEYGMSQFNIKHTVYSTQQFDFVK V*QNN*LFWITQHVFLKCCFSIFYLLISTIF*I LYKKLPSAL*TNVEN*CSCFVLCSGKALSECLT PFLYFLVIHIILL*VLQQS*IIQIITTERGTFVF LNVLFHITIICVVVELCYQSMSNFCPDDIKTKA *LLSLLF*SP*QCYGISSIMM**KDFSRKHFEI LKEIQSISNVMNEE*NNYRKRMHDFSLSHQITSW TILHFQNGELFKVDV*PSGICT*LAF*NNSLNI GFILSRCQDEHNVNIISISKQLNDTVRFFGVCF VLSISSIYPNSAHRHEEAGCRFKGTQGENKCSFH SVRINVESW*YWLLNWPAPETGSKKKEATKTKC TA*MDVALSCGIWKIFNYPNVISLALMLYFRVP GTTG*NITSVVILFEMGVGTN*NMIENYMQILKS SQY*HL*RAFLKFKQLGDAS*YYKLKTILRGK* LLFSFCPFSQKHLSPKCMSLGHFIVLRGRKQKR GIIWEEPR*NLIFWTLNLLVK*C*SLYHLSYMF* PLFFF*LFHPKFLVNYTEIFGNGTLCR*PPCTS GNVPVTAVDYPTLQEIHSEMS*QSPV*QESQSL PSPGPKAPQGWYSLSHRY***KPQCSFSM*FLSY KPGGFWS*LKRWEFT*GQEVPRMPKQPHRCPVL PVRLPGGNIQTNIFFNVYVIYTAAFLIFCF*LL VVNHLFGLQALSSICIFQIYHE*SCSTVKDVKPK K**SFYLAVEP*CSPYYIHYKLCLLATTESPQT F*L*VEAIRRCPYGLRNNSSNVLA*FV*MSLF* Y*HRHKSIFATR**LFSFALFSSALY*SVTQC*S SFFCVGNLRHVNICKS*QSKVQTIFRDFGECSD QKWLIYFISDYFIQRVLF*WIFVNLPLNH*KLF CFNPTLPSIPLPPQKAPINLLIPLM*LVECE*IT WKK

Frame 1

LVAPASRPGRGDLSASRPVRSAAPAPQSVCKWP SPPPTPAAGAGAVSCTLRGYRRSRGCLLPVLCA NPGARPRGTRERLRLHSAQVLGFPLGHPKCFLNT LDKS*TTCHWGLPYSRLPCHLMAF*LSRGQVTC CP**VMRRTLVCPKNWYHLQGAQVAALPSKEVD RTAGKWWKD*GGR*ITPRSHQFRKDFC*KRGSGP *KAGIRDSSIWTKES*NMPRAKPI*RERSCMAA LMSGSQ*CL*RSHQNA*TLTPRSTSTI*RSSQK KSLMSGYRNFATTECIVRMKLPCFHMKLTTFSQG PPSQTLHLGCLTPFQVGSVAVYQSRIYFKLEAM YHFLVVVRHEFHYGYSLQRTWKNAPKTWRTVMP TW*K*ASSCKAWTSCIGHTRHQLSTPSRSRNLFL AQ*DYTPPILICQH*ILEKRKIILMALKPHQSF LKCKKICVILPIKFTSL*GQLLISCQRRERN*S S*WSRMPPPPRLLRSLV*RTPCHPKTPEMKTEL* FISFLMKVDSPSLTPFLSFLMLRKFCYLQALQK TRFLMMTHMSVT*VIIFP*IISVMI*IMRDRPW GLSLIVVGKRSPGEERACRRPARAAVTSACGTS* GTTSGRTCPRWPCRWS*TSP*TRCRGSARSWST ASSWTRPRRFPAPWKGWYMWQPLPYQRMHLATT ELEASHLIRFLEKHMNVFGRTRASSFFQNRSATI RLSLRVMLSLEILFSGKM*DGKTNSGANPWKLF QLAQPM*LCQFLGIILSGTK*PLASITS*AGRG GLSTMERLSSRTCMMIPATAK*IL*RQNTGALMP MRLKAQCLTGVEKRFIGCLGNGMKASTVAAAPL LPVYGEQILCRKATSNTIASHSLRWN*MKWIHH QSLYCHLLTLDLGQTRGF*RKGT*KKLKYKSRGL NNCREKGGGS*KKIMWSTSLGFSGNPTMTLG*A TAPIWNLEKILVFPNWTILSYGEKVKKKDNISV FLLCLPSEVAQTCVYIFKRYSRMITCA*LSIVTL *VYIFLSAFLYNFKCYPDCLYECRTP*HFFLYI NYLIKMKD*MFMCGLKKEALTLIFQRLGKIPIK FMPYKIML*KKNQPLPGALRNKNSQGYSPMRKLP KFNLVAEISFCLRQLLNCSYRTILLVLESEEYF HLHFSG*GEDIRLMECIFLYKTYTAPS*KGSI* TCSSL*FYCLICKIVPCCSEKLFIREKNSN*LNI TIASQRDSRKFLLSES*VLEKLRDCFLLPTPPR FLSLRLITSLLSMECPSLI*NIQFIPHSSLTL* KFSKITDCFG*LNMFFLNAVLVFSTS**APYFRS CIKSCHLPYRQM*RIDVLALCCVLVKL*VSVLP LSYTFLLSISFFCEFCSSLE*YRL*LLKGEPSS F*MCYFTLQ*YV**LNCVIKACPTSVQMTLKPKP NC*ASYSKVHSNAMEYQV**CSKKISPENTLRY LKKFRAYRML*MKSKIITGKECMIFHCHTK*PV GQYCIFKMESYLKWMCSLLESARDWHFKTTH*IL DSFYRDAKMNTM*ISFPFLSS*MTQSDSLVYAL FFPSQAFIQILLTDMRKQAVDLRALKGKTNVVS TV*G*M*NRGNIGC*IGLLQRLAQKRKKQRKQNA QPEWT*HCHVAFGKSLIILMLFHLPLCFILESQ GQLDETLRL*SSYLRWGWERIKI**RTICRF*N LVSTSIYKGLF*NLNSLVMHPDIISLKLS*GGND FFSPSVPFLKSIFPPNVCL*DIL*F*EEENRNV ASFGKNQGRI*FSGL*ISWLNNANHCTI*VTCF NLFFFSNYSIQNFW*ITQKFLEMGLCAGNHPALQ VMFLSQLSTTPRCRKSIQK*ANSLQCSRRASHF PPQAPKHPKAGIP*VIGINNKSLNAASPCSSSP TSQVDSGPN*RDGSSPEGKKYPGCPNSRTGVLCF L*DFLGEIYKLIYFLMFTSFTLLLFSYSAFNYL **IICLGFKHCLPSASSRFIMSDLVLLLKMSSL KSNKVFIWLLNLDVAPTTYTTSYAFWLLQNLRRP FSYE*KQ*EGVLMVLGIIVPMYWHNLFECLYFD INTGINLFLLPDDSYFHLLYFPLHCTNLLLSVS LHFSVLETCVM*ISANHDSLKCKPFSETLGNAVI RSG*FILFLIILSRGYFFNGYL*IFHLTTENYF VLIPPFHPYLCLPKKLLLICLSPSCS*LNVNK* HGKK

Frame 2

WWRPPAGPGEGT*ALRGRCAARLRPRSLSASGH PRPRLLRPVRELSAARCGGTGEAGVAFSRFCAL TPEPGPGELGSVSGCTALRF*GFLWATQNVF*I LWIRAKPPATGDFHTLGSLVI*WHFDCLEAK*LA VHDE**EEPWCVPKIGITFKEHK*LLFQARKST GQLGSGGRTEGGDELHPGATSSERIFAEKEEVA LKRLA*EILLSGQRNLEICQEPNRYREREAAWLH *CRALSDVCKEVIKMHRP*HRGAHLPSEGQVRR SL**VGIETSPPQNVSSE*NCHVST*S*PLFLR VHHHRLFIWGV*LHFK*EA*QYIKAEFISNWKQC IIFLWW*DTSSIMVTVFRGHGKMLQRPGALSCL PGRNEPAPAKHGRPASDILGTSYQRHPGPETFF WPSKTTLLQS*FVNTRFWRREKLF*WL*NLIRVF *NARRSVSYCP*SLLHFKVSF*YHVSGERETEA ADGAGCLLLPVCSGHWSEERPVIRKLQR*KPSS SSSAF**K*TLHH*LPF*VF*CSGSSVISKLFRK RDF***LICQ*HK**SFLR*SQ**FR**ETDLG ACP**WSGSEVPEKNVPAGALPEQQ*HQPVEHP EEQHREGPVQGGHAGGAERAPEHAAEALRGAGVQ RAPGQGRADSQPPGKDGICGSLCHISVCI*LLP SWKQAI*SGSWRNI*MYSGGQGLPVFFRTGQPP SAYLCVSC*V*KFCFLARCEMEKQILGQIHGNCS NWHNPCDSASFWGSF*VEQSDLLHP*HLKRAEV D*ALWRDCHQEPA**FLLLQSEFYKGKILEH*C P*D*RHSV*QEWKSGSSAVWEMA*KHLLWRRLLF CLCMESKSYAERLRAIL*LHTVCAGIK*NGSII KVFIATY*HSI*ARPEVSRGRELRRS*NTKAED *TTAERKAAGLRRKSCGAPASVFQEIRR*LLGEQ RHLFGT*KRSWFFQTGPSCLMVKK*RRKITLVY FSCACLLKWHKPVFIYLKDTLG*SLVLSLAL*L FKSIFSSVRFFTISNVTLIVYMNVEHLDISFYI* TI**K*KIECSCVG*KKKL*H*FSKG*GRFQLN LCLIKLCCRKKINLSQVH*EIRIPRVTHPCVSY PSLIW*LKYLFASDSS*IAHTEQFCWCWSLKNIF ICILVVRERI*D*WNVYFYIRRIRHLLEKEAFE LVPPYSSIALYAKLYPVAQRNYS*EKRIPIN*I SQ*HPRETVGNFSLVRAESLRS*ETVSCFPPHPV FCPSV*SPPC*VWNVPV*YKTYSLFHTAV*LCK SLAK*LIVLDNSTCFS*MLF*YFLPLNKHHILD LV*KVAICLIDKCRELMFLLCVVFW*SFK*VSYP FPILSCYPYHSFVSFAAVLNNTDYNY*KGNLRL FKCVISHYNNMCSS*IVLSKHVQLLSR*H*NQS LIVKPLILKSIAMLWNIKYNDVVKRFLQKTL*DT *RNSEHIECYE*RVK*LQEKNA*FFIVTPNNQL DNIAFSKWRVI*SGCVAFWNLHVTGILKQLIKY WIHFIEMPR*TQCEYHFHF*AVE*HSQILWCMLC SFHLKHLSKFCSQT*GSRL*I*GHSRGKQM*FP QCEDKCRIVVILAVKLACSRDWLKKERSNENKM HSLNGRSTVMWHLENL*LS*CYFTCPYALF*SPR DNWMKHYVCSHLI*DGGGNELKYDRELYADFKI *SVLASIKGFSEI*TAW*CILIL*A*NYLEGEM TSFLLLSLFSKASFPQMYVSRTFYSFKRKKTETW HHLGRTKVEFNFLDFKSPG*IMLITVPFELHVL TSFFFLIIPSKISGELHRNFWKWDFVQVTTLHF R*CSCHSCRLPHAAGNPFRNELTVSSVAGEPVTS LPRPQSTPRLVFLES*VLIIKASMQLLHVVPLL QARWILVLTKEMGVHLRARSTQDAQTAAQVSCA SCETSWGKYTN*YIF*CLRHLHCCFSHILLLITC SKSFVWASSTVFHLHLPDLS*VILFYC*RCQA* KVIKFLFGC*TLM*PLLHTLQVMPSGYYRISAD LLVMSRSNKKVSLWS*E**FQCIGIICLNVFILI LTPA*IYFCYQMIAIFICSIFLCIVLICYSVLV FIFLCWKPASCKYLQIMTV*SANHFQRLWGMQ* SEVANLFYF*LFYPEGTFLMDICKSST*PLKIIL F*SHPSIHTSASPKSSY*FAYPPHVAS*M*INN MEK

Sequence NM_015550

GCTGGTGGCGCCCGCCAGCCGGCCCGGGAGAGGGGACTTGAGCGCTTCGCGGCCGGTGCGCAGCGCGGCTCCGGCCCCGCAGTCTGTCTGCAAGTGGCCA TCCCCGCCCCCGACTCCTGCGGCCGGTGCGGGAGCTGTCAGCTGCACGTTGCGGGGGTACCGGAGAAGCCGGGGTTGCCTTCTCCCGGTTCTGTGCGCTA ACCCCGGAGCCCGGCCCCGGGGAACTAGGGAGCGTCTCCGGCTGCACAGCGCTCAGGTTTTAGGGTTTCCTTTGGGCCACCCAAAATGTTTTTTAAATAC TTTGGATAAGAGCTAAACCACCTGCCACTGGGGACTTCCATACTCTCGGCTCCCTTGTCATCTGATGGCATTTTGATTGTCTCGAGGCCAAGTGACTTGC TGTCCATGATGAGTGATGAGAAGAACCTTGGTGTGTCCCAAAAATTGGTATCACCTTCAAGGAGCACAAGTAGCTGCTCTTCCAAGCAAGGAAGTCGACA GGACAGCTGGGAAGTGGTGGAAGGACTGAGGGGGGAGATGAATTACACCCAGGAGCCACCAGTTCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCC TTAAAAGGCTGGCATAAGAGATTCTTCTATCTGGACAAAGGAATCTTGAAATATGCCAAGAGCCAAACCGATATAGAGAGAGAGAAGCTGCATGGCTGCA TTGATGTCGGGCTCTCAGTGATGTCTGTAAAGAAGTCATCAAAATGCATAGACCTTGACACCGAGGAGCACATCTACCATCTGAAGGTCAAGTCAGAAGA AGTCTTTGATGAGTGGGTATCGAAACTTCGCCACCACAGAATGTATCGTCAGAATGAAATTGCCATGTTTCCACATGAAGTTAACCACTTTTTCTCAGGG TCCACCATCACAGACTCTTCATCTGGGGTGTTTGACTCCATTTCAAGTAGGAAGCGTAGCAGTATATCAAAGCAGAATTTATTTCAAACTGGAAGCAATG TATCATTTTCTTGTGGTGGTGAGACACGAGTTCCATTATGGTTACAGTCTTCAGAGGACATGGAAAAATGCTCCAAAGACCTGGCGCACTGTCATGCCTA CCTGGTAGAAATGAGCCAGCTCCTGCAAAGCATGGACGTCCTGCATCGGACATACTCGGCACCAGCTATCAACGCCATCCAGGGTGGATCTTTTGAAAGT CCCAAAAAGGAAAAAAGATCGCACAGGAGGTGGCGGTCCAGAGCTATTGGCAAAGATGCTAAAGGAACACTGCAGGTCCCGAAACCTTTTTCTGGCCCAG TAAGACTACACTCCTCCAATCCTAATTTGTCAACACTAGATTTTGGAGAAGAGAAAAATTATTCTGATGGCTCTGAAACCTCATCAGAGTTTTCTAAAAT GCAAGAAGATCTGTGTCATATTGCCCATAAAGTTTACTTCACTTTAAGGTCAGCTTTTAATATCATGTCAGCGGAGAGAGAGAAACTGAAGCAGCTGATG GAGCAGGATGCCTCCTCCTCCCCGTCTGCTCAGGTCATTGGTCTGAAGAACGCCCTGTCATCCGCCCTAGCACAAAACACAGATCTTAAAGAACGCTTAC GCAGAATCCATGCCGAGTCTCTGCTCCTCGACTCCCCCGCTGTCGCCAAGTCGGGTGACAATCTGGCAGAGGAAAACTCCAGAGATGAAAACCGAGCTCT AGTTCATCAGCTTTCTAATGAAAGTAGACTCTCCATCACTGACTCCCTTTCTGAGTTTTTTGATGCTCAGGAAGTTCTGTTATCTCCAAGCTCTTCAGAA AACGAGATTTCTGATGATGACTCATATGTCAGTGACATAAGTGATAATCTTTCCTTAGATAATCTCAGTAATGATTTAGATAATGAGAGACAGACCTTGG GGCCTGTCCTTGATAGTGGTCGGGAAGCGAAGTCCCGGAGAAGAACGTGCCTGCCGGCGCCCTGCCCGAGCAGCAGTAACATCAGCCTGTGGAACATCCT GAGGAACAACATCGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGAGGCTCTGCGAGGAGCTGGAGTAC AGCGAGCTCCTGGACAAGGCCGCGCAGATTCCCAGCCCCCTGGAAAGGATGGTATATGTGGCAGCCTTTGCCATATCAGCGTATGCATCTAGCTACTACC GAGCTGGAAGCAAGCCATTTAATCCGGTTCTTGGAGAAACATATGAATGTATTCGGGAGGACAAGGGCTTCCAGTTTTTTTCAGAACAGGTCAGCCACCA TCCGCCTATCTCTGCGTGTCATGCTGAGTCTAGAAATTTTGTTTTCTGGCAAGATGTGAGATGGAAAAACAAATTCTGGGGCAAATCCATGGAAATTGTT CCAATTGGCACAACCCATGTGACTCTGCCAGTTTTTGGGGATCATTTTGAGTGGAACAAAGTGACCTCTTGCATCCATAACATCTTAAGCGGGCAGAGGT GGATTGAGCACTATGGAGAGATTGTCATCAAGAACCTGCATGATGATTCCTGCTACTGCAAAGTGAATTTTATAAAGGCAAAATACTGGAGCACTAATGC CCATGAGATTGAAGGCACAGTGTTTGACAGGAGTGGAAAAGCGGTTCATCGGCTGTTTGGGAAATGGCATGAAAGCATCTACTGTGGCGGCGGCTCCTCT TCTGCCTGTGTATGGAGAGCAAATCCTATGCCGAAAGGCTACGAGCAATACTATAGCTTCACACAGTTTGCGCTGGAATTAAATGAAATGGATCCATCAT CAAAGTCTTTATTGCCACCTACTGACACTCGATTTAGGCCAGACCAGAGGTTTCTAGAGGAAGGGAACTTAGAAGAAGCTGAAATACAAAAGCAGAGGAT TGAACAACTGCAGAGAGAAAGGCGGCGGGTCTTAGAAGAAAATCATGTGGAGCACCAGCCTCGGTTTTTCAGGAAATCCGACGATGACTCTTGGGTGAGC AACGGCACCTATTTGGAACTTAGAAAAGATCTTGGTTTTTCCAAACTGGACCATCCTGTCTTATGGTGAAAAAGTAAAGAAGAAAGATAACATTAGTGTA TTTCTCCTGTGCTTGCCTTCTGAAGTGGCACAAACCTGTGTTTATATATTTAAAAGATACTCTAGGATGATCACTTGTGCTTAGCTTAGCATTGTAACTC TTTAAGTCTATATTTTCCTCAGTGCGTTTCTTTACAATTTCAAATGTTACCCTGATTGTTTATATGAATGTAGAACACCTTGACATTTCTTTTTATATAT AAACTATTTAATAAAAATGAAAGATTGAATGTTCATGTGTGGGTTAAAAAAAGAAGCTTTAACACTAATTTTCCAAAGGTTAGGGAAGATTCCAATTAAA TTTATGCCTTATAAAATTATGTTGTAGAAAAAAAATCAACCTCTCCCAGGTGCATTAAGAAATAAGAATTCCCAGGGTTACTCACCCATGCGTAAGCTAC CCAAGTTTAATTTGGTAGCTGAAATATCTTTTTGCCTCAGACAGCTCTTGAATTGCTCATACAGAACAATTCTGCTGGTGCTGGAGTCTGAAGAATATTT TCATTTGCATTTTAGTGGTTAGGGAGAGGATATAAGATTAATGGAATGTATATTTTTATATAAGACGTATACGGCACCTTCTTGAAAAGGAAGCATTTGA ACTTGTTCCTCCCTATAGTTCTATTGCCTTATATGCAAAATTGTACCCTGTTGCTCAGAGAAATTATTCATAAGAGAAAAGAATTCCAATTAATTAAATA TCACAATAGCATCCCAGAGAGACAGTAGGAAATTTCTCCTTAGTGAGAGCTGAGTCCTTGAGAAGTTAAGAGACTGTTTCCTGCTTCCCACCCCACCCCG TTTTTTGTCCCTCCGTTTGATCACCTCCCTGTTGAGTATGGAATGTCCCAGTTTAATATAAAACATACAGTTTATTCCACACAGCAGTTTGACTTTGTAA AAGTTTAGCAAAATAACTGATTGTTTTGGATAACTCAACATGTTTTTCTTAAATGCTGTTTTAGTATTTTCTACCTCTTAATAAGCACCATATTTTAGAT CTTGTATAAAAAGTTGCCATCTGCCTTATAGACAAATGTAGAGAATTGATGTTCTTGCTTTGTGTTGTGTTCTGGTAAAGCTTTAAGTGAGTGTCTTACC CCTTTCCTATACTTTCTTGTTATCCATATCATTCTTTTGTGAGTTTTGCAGCAGTCTTGAATAATACAGATTATAACTACTGAAAGGGGAACCTTCGTCT TTTTAAATGTGTTATTTCACATTACAATAATATGTGTAGTAGTTGAATTGTGTTATCAAAGCATGTCCAACTTCTGTCCAGATGACATTAAAACCAAAGC CTAATTGTTAAGCCTCTTATTCTAAAGTCCATAGCAATGCTATGGAATATCAAGTATAATGATGTAGTAAAAAGATTTCTCCAGAAAACACTTTGAGATA CTTAAAGAAATTCAGAGCATATCGAATGTTATGAATGAAGAGTAAAATAATTACAGGAAAAGAATGCATGATTTTTCATTGTCACACCAAATAACCAGTT GGACAATATTGCATTTTCAAAATGGAGAGTTATTTAAAGTGGATGTGTAGCCTTCTGGAATCTGCACGTGACTGGCATTTTAAAACAACTCATTAAATAT TGGATTCATTTTATCGAGATGCCAAGATGAACACAATGTGAATATCATTTCCATTTCTAAGCAGTTGAATGACACAGTCAGATTCTTTGGTGTATGCTTT GTTCTTTCCATCTCAAGCATTTATCCAAATTCTGCTCACAGACATGAGGAAGCAGGCTGTAGATTTAAGGGCACTCAAGGGGAAAACAAATGTAGTTTCC ACAGTGTGAGGATAAATGTAGAATCGTGGTAATATTGGCTGTTAAATTGGCCTGCTCCAGAGACTGGCTCAAAAAAGAAAGAAGCAACGAAAACAAAATG CACAGCCTGAATGGACGTAGCACTGTCATGTGGCATTTGGAAAATCTTTAATTATCCTAATGTTATTTCACTTGCCCTTATGCTTTATTTTAGAGTCCCA GGGACAACTGGATGAAACATTACGTCTGTAGTCATCTTATTTGAGATGGGGGTGGGAACGAATTAAAATATGATAGAGAACTATATGCAGATTTTAAAAT CTAGTCAGTACTAGCATCTATAAAGGGCTTTTCTGAAATTTAAACAGCTTGGTGATGCATCCTGATATTATAAGCTTAAAACTATCTTGAGGGGGAAATG ACTTCTTTTCTCCTTCTGTCCCTTTTCTCAAAAGCATCTTTCCCCCAAATGTATGTCTCTAGGACATTTTATAGTTTTAAGAGGAAGAAAACAGAAACGT GGCATCATTTGGGAAGAACCAAGGTAGAATTTAATTTTCTGGACTTTAAATCTCCTGGTTAAATAATGCTAATCACTGTACCATTTGAGTTACATGTTTT AACCTCTTTTTTTTTTCTAATTATTCCATCCAAAATTTCTGGTGAATTACACAGAAATTTTTGGAAATGGGACTTTGTGCAGGTAACCACCCTGCACTTC AGGTAATGTTCCTGTCACAGCTGTCGACTACCCCACGCTGCAGGAAATCCATTCAGAAATGAGCTAACAGTCTCCAGTGTAGCAGGAGAGCCAGTCACTT CCCTCCCCAGGCCCCAAAGCACCCCAAGGCTGGTATTCCTTGAGTCATAGGTATTAATAATAAAAGCCTCAATGCAGCTTCTCCATGTAGTTCCTCTCCT ACAAGCCAGGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACCTGAGGGCAAGAAGTACCCAGGATGCCCAAACAGCCGCACAGGTGTCCTGTGCT TCCTGTGAGACTTCCTGGGGGAAATATACAAACTAATATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCATATTCTGCTTTTAATTACTT GTAGTAAATCATTTGTTTGGGCTTCAAGCACTGTCTTCCATCTGCATCTTCCAGATTTATCATGAGTGATCTTGTTCTACTGTTAAAGATGTCAAGCCTA AAAAGTAATAAAGTTTTTATTTGGCTGTTGAACCTTGATGTAGCCCCTACTACATACACTACAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGAC CTTTTAGTTATGAGTAGAAGCAATAAGAAGGTGTCCTTATGGTCTTAGGAATAATAGTTCCAATGTATTGGCATAATTTGTTTGAATGTCTTTATTTTGA TATTAACACCGGCATAAATCTATTTTTGCTACCAGATGATAGCTATTTTCATTTGCTCTATTTTCCTCTGCATTGTACTAATCTGTTACTCAGTGTTAGT CTTCATTTTTCTGTGTTGGAAACCTGCGTCATGTAAATATCTGCAAATCATGACAGTCTAAAGTGCAAACCATTTTCAGAGACTTTGGGGAATGCAGTGA TCAGAAGTGGCTAATTTATTTTATTTCTGATTATTTTATCCAGAGGGTACTTTTTTAATGGATATTTGTAAATCTTCCACTTAACCACTGAAAATTATTT TGTTTTAATCCCACCCTTCCATCCATACCTCTGCCTCCCCAAAAAGCTCCTATTAATTTGCTTATCCCCCTCATGTAGCTAGTTGAATGTGAATAAATAA CATGGAAAAAAA

refseq_OSBPL3.F2 (forward primer)
refseq_OSBPL3.R2 (reverse primer)
Single exon skipping

Frame 0

AGGARQPARERGLERFAAGAQRGSGPAVCLQVA IPAPDSCGRCGSCQLHVAGVPEKPGLPSPGSVR* PRSPAPGN*GASPAAQRSGFRVSFGPPKMFFKY FG*ELNHLPLGTSILSAPLSSDGILIVSRPSDL LSMMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQ DSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWP LKGWHKRFFYLDKGILKYAKSQTDIEREKLHGC IDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEE VFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSG STITDSSSGVFDSISSRKRSSISKQNLFQTGSN VSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAY LVEMSQLLQSMDVLHRTYSAPAINAIQGGSFES PKKEKRSHRRWRSRAIGKDAKGTLQVPKPFSGP VRLHSSNPNLSTLDFGEEKNYSDGSETSSEFSKM QEDLCHIAHKVYFTLRSAFNIMSAEREKLKQLM EQDASSSPSAQVIGLKNALSSALAQNTDLKERL RRIHAESLLLDSPAVAKSGDNLAEENSRDENRAL VHQLSNESRLSITDSLSEFFDAQEVLLSPSSSE NEISDDDSYVSDISDNLSLDNLSNDLDNERQTL GPVLDSGREAKSRRRTCLPAPCPSSSNISLWNIL RNNIGKDLSKVAMPVELNEPLNTLQRLCEELEY SELLDKAAQIPSPLERMVYVAAFAISAYASSYY RAGSKPFNPVLGETYECIREDKGFQFFSEQVSHH PPISACHAESRNFVFWQDVRWKNKFWGKSMEIV PIGTTHVTLPVFGDHFEWNKVTSCIHNILSGQR WIEHYGEIVIKNLHDDSCYCKVNFIKAKYWSTNA HEIEGTVFDRSGKAVHRLFGKWHESIYCGGGSS SACVWRANPMPKGYEQYYSFTQFALELNEMDPS SKSLLPPTDTRFRPDQRFLEEGNLEEAEIQKQRI EQLQRERRRVLEENHVEHQPRFFRKSDDDSWVS NGTYLELRKDLGFSKLDHPVLW*KSKEER*H*C ISPVLAF*SGTNLCLYI*KIL*DDHLCLA*HCNS LSLYFPQCVSLQFQMLP*LFI*M*NTLTFLFIY KLFNKNERLNVHVWVKKRSFNTNFPKVREDSN* IYAL*NYVVEKKSTSPRCIKK*EFPGLLTHA*AT QV*FGS*NIFLPQTALELLIQNNSAGAGV*RIF SFAF*WLGRGYKINGMYIFI*DVYGTFLKRKHL NLFLPIVLLPYMQNCTLLLREIIHKRKEFQLIKY HNSIPERQ*EISP**ELSP*EVKRLFPASHPTP FFVPPFDHLPVEYGMSQFNIKHTVYSTQQFDFV KV*QNN*LFWITQHVFLKCCFSIFYLLISTIF*I LYKKLPSAL*TNVEN*CSCFVLCSGKALSECLT PFLYFLVIHIILL*VLQQS*IIQIITTERGTFV FLNVLFHITIICVVVELCYQSMSNFCPDDIKTKA *LLSLLF*SP*QCYGISSIMM**KDFSRKHFEI LKEIQSISNVMNEE*NNYRKRMHDFSLSHQITS WTILHFQNGELFKVDV*PSGICT*LAF*NNSLNI GFILSRCQDEHNVNIISISKQLNDTVRFFGVCF VLSISSIYPNSAHRHEEAGCRFKGTQGENKCSF HSVRINVESW*YWLLNWPAPETGSKKKEATKTKC TA*MDVALSCGIWKIFNYPNVISLALMLYFRVP GTTG*NITSVVILFEMGVGTN*NMIENYMQILK SSQY*HL*RAFLKFKQLGDAS*YYKLKTILRGK* LLFSFCPFSQKHLSPKCMSLGHFIVLRGRKQKR GIIWEEPR*NLIFWTLNLLVK*C*SLYHLSYMF *PLFFF*LFHPKFLVNYTEIFGNGTLCR*PPCTS GNVPVTAVDYPTLQEIHSEMS*QSPV*QESQSL PSPGPKAPQGWYSLSHRY***KPQCSFSM*FLS YKPGGFWS*LKRWEFT*GQEVPRMPKQPHRCPVL PVRLPGGNIQTNIFFNVYVIYTAAFLIFCF*LL VVNHLFGLQALSSICIFQIYHE*SCSTVKDVKP KK**SFYLAVEP*CSPYYIHYKLCLLATTESPQT F*L*VEAIRRCPYGLRNNSSNVLA*FV*MSLF* Y*HRHKSIFATR**LFSFALFSSALY*SVTQC* SSFFCVGNLRHVNICKS*QSKVQTIFRDFGECSD QKWLIYFISDYFIQRVLF*WIFVNLPLNH*KLF CFNPTLPSIPLPPQKAPINLLIPLM*LVECE*I TWKK

Frame 1

LVAPASRPGRGDLSASRPVRSAAPAPQSVCKWP SPPPTPAAGAGAVSCTLRGYRRSRGCLLPVLCA NPGARPRGTRERLRLHSAQVLGFPLGHPKCFLNT LDKS*TTCHWGLPYSRLPCHLMAF*LSRGQVTC CP**VMRRTLVCPKNWYHLQGAQVAALPSKEVD RTAGKWWKD*GGR*ITPRSHQFRKDFC*KRGSGP *KAGIRDSSIWTKES*NMPRAKPI*RERSCMAA LMSGSQ*CL*RSHQNA*TLTPRSTSTI*RSSQK KSLMSGYRNFATTECIVRMKLPCFHMKLTTFSQG PPSQTLHLGCLTPFQVGSVAVYQSRIYFKLEAM YHFLVVVRHEFHYGYSLQRTWKNAPKTWRTVMP TW*K*ASSCKAWTSCIGHTRHQLSTPSRVDLLKV PKRKKDRTGGGGPELLAKMLKEHCRSRNLFLAQ *DYTPPILICQH*ILEKRKIILMALKPHQSFLK CKKICVILPIKFTSL*GQLLISCQRRERN*SS*W SRMPPPPRLLRSLV*RTPCHPP*HKTQILKNAY AESMPSLCSSTPPLSPSRVTIWQRKTPEMKTEL *FISFLMKVDSPSLTPFLSFLMLRKFCYLQALQK TRFLMMTHMSVT*VIIFP*IISVMI*IMRDRPW GLSLIVVGKRSPGEERACRRPARAAVTSACGTS *GTTSGRTCPRWPCRWS*TSP*TRCRGSARSWST ASSWTRPRRFPAPWKGWYMWQPLPYQRMHLATT ELEASHLIRFLEKHMNVFGRTRASSFFQNRSAT IRLSLRVMLSLEILFSGKM*DGKTNSGANPWKLF QLAQPM*LCQFLGIILSGTK*PLASITS*AGRG GLSTMERLSSRTCMMIPATAK*IL*RQNTGALM PMRLKAQCLTGVEKRFIGCLGNGMKASTVAAAPL LPVYGEQILCRKATSNTIASHSLRWN*MKWIHH QSLYCHLLTLDLGQTRGF*RKGT*KKLKYKSRG LNNCREKGGGS*KKIMWSTSLGFSGNPTMTLG*A TAPIWNLEKILVFPNWTILSYGEKVKKKDNISV FLLCLPSEVAQTCVYIFKRYSRMITCA*LSIVT L*VYIFLSAFLYNFKCYPDCLYECRTP*HFFLYI NYLIKMKD*MFMCGLKKEALTLIFQRLGKIPIK FMPYKIML*KKNQPLPGALRNKNSQGYSPMRKL PKFNLVAEISFCLRQLLNCSYRTILLVLESEEYF HLHFSG*GEDIRLMECIFLYKTYTAPS*KGSI* TCSSL*FYCLICKIVPCCSEKLFIREKNSN*LN ITIASQRDSRKFLLSES*VLEKLRDCFLLPTPPR FLSLRLITSLLSMECPSLI*NIQFIPHSSLTL* KFSKITDCFG*LNMFFLNAVLVFSTS**APYFR SCIKSCHLPYRQM*RIDVLALCCVLVKL*VSVLP LSYTFLLSISFFCEFCSSLE*YRL*LLKGEPSS F*MCYFTLQ*YV**LNCVIKACPTSVQMTLKPK PNC*ASYSKVHSNAMEYQV**CSKKISPENTLRY LKKFRAYRML*MKSKIITGKECMIFHCHTK*PV GQYCIFKMESYLKWMCSLLESARDWHFKTTH*I LDSFYRDAKMNTM*ISFPFLSS*MTQSDSLVYAL FFPSQAFIQILLTDMRKQAVDLRALKGKTNVVS TV*G*M*NRGNIGC*IGLLQRLAQKRKKQRKQN AQPEWT*HCHVAFGKSLIILMLFHLPLCFILESQ GQLDETLRL*SSYLRWGWERIKI**RTICRF*N LVSTSIYKGLF*NLNSLVMHPDIISLKLS*GGN DFFSPSVPFLKSIFPPNVCL*DIL*F*EEENRNV ASFGKNQGRI*FSGL*ISWLNNANHCTI*VTCF NLFFFSNYSIQNFW*ITQKFLEMGLCAGNHPAL QVMFLSQLSTTPRCRKSIQK*ANSLQCSRRASHF PPQAPKHPKAGIP*VIGINNKSLNAASPCSSSP TSQVDSGPN*RDGSSPEGKKYPGCPNSRTGVLC FL*DFLGEIYKLIYFLMFTSFTLLLFSYSAFNYL **IICLGFKHCLPSASSRFIMSDLVLLLKMSSL KSNKVFIWLLNLDVAPTTYTTSYAFWLLQNLRR PFSYE*KQ*EGVLMVLGIIVPMYWHNLFECLYFD INTGINLFLLPDDSYFHLLYFPLHCTNLLLSVS LHFSVLETCVM*ISANHDSLKCKPFSETLGNAV IRSG*FILFLIILSRGYFFNGYL*IFHLTTENYF VLIPPFHPYLCLPKKLLLICLSPSCS*LNVNK* HGKK

Frame 2

WWRPPAGPGEGT*ALRGRCAARLRPRSLSASGH PRPRLLRPVRELSAARCGGTGEAGVAFSRFCAL TPEPGPGELGSVSGCTALRF*GFLWATQNVF*I LWIRAKPPATGDFHTLGSLVI*WHFDCLEAK*LA VHDE**EEPWCVPKIGITFKEHK*LLFQARKST GQLGSGGRTEGGDELHPGATSSERIFAEKEEVA LKRLA*EILLSGQRNLEICQEPNRYREREAAWLH *CRALSDVCKEVIKMHRP*HRGAHLPSEGQVRR SL**VGIETSPPQNVSSE*NCHVST*S*PLFLR VHHHRLFIWGV*LHFK*EA*QYIKAEFISNWKQC IIFLWW*DTSSIMVTVFRGHGKMLQRPGALSCL PGRNEPAPAKHGRPASDILGTSYQRHPGWIF*K SQKGKKIAQEVAVQSYWQRC*RNTAGPETFFWPS KTTLLQS*FVNTRFWRREKLF*WL*NLIRVF*N ARRSVSYCP*SLLHFKVSF*YHVSGERETEAAD GAGCLLLPVCSGHWSEERPVIRPSTKHRS*RTLT QNPCRVSAPRLPRCRQVG*QSGRGKLQR*KPSS SSSAF**K*TLHH*LPF*VF*CSGSSVISKLFR KRDF***LICQ*HK**SFLR*SQ**FR**ETDLG ACP**WSGSEVPEKNVPAGALPEQQ*HQPVEHP EEQHREGPVQGGHAGGAERAPEHAAEALRGAGV QRAPGQGRADSQPPGKDGICGSLCHISVCI*LLP SWKQAI*SGSWRNI*MYSGGQGLPVFFRTGQPP SAYLCVSC*V*KFCFLARCEMEKQILGQIHGNC SNWHNPCDSASFWGSF*VEQSDLLHP*HLKRAEV D*ALWRDCHQEPA**FLLLQSEFYKGKILEH*C P*D*RHSV*QEWKSGSSAVWEMA*KHLLWRRLL FCLCMESKSYAERLRAIL*LHTVCAGIK*NGSII KVFIATY*HSI*ARPEVSRGRELRRS*NTKAED *TTAERKAAGLRRKSCGAPASVFQEIRR*LLGE QRHLFGT*KRSWFFQTGPSCLMVKK*RRKITLVY FSCACLLKWHKPVFIYLKDTLG*SLVLSLAL*L FKSIFSSVRFFTISNVTLIVYMNVEHLDISFYI *TI**K*KIECSCVG*KKKL*H*FSKG*GRFQLN LCLIKLCCRKKINLSQVH*EIRIPRVTHPCVSY PSLIW*LKYLFASDSS*IAHTEQFCWCWSLKNI FICILVVRERI*D*WNVYFYIRRIRHLLEKEAFE LVPPYSSIALYAKLYPVAQRNYS*EKRIPIN*I SQ*HPRETVGNFSLVRAESLRS*ETVSCFPPHP VFCPSV*SPPC*VWNVPV*YKTYSLFHTAV*LCK SLAK*LIVLDNSTCFS*MLF*YFLPLNKHHILD LV*KVAICLIDKCRELMFLLCVVFW*SFK*VSY PFPILSCYPYHSFVSFAAVLNNTDYNY*KGNLRL FKCVISHYNNMCSS*IVLSKHVQLLSR*H*NQS LIVKPLILKSIAMLWNIKYNDVVKRFLQKTL*D T*RNSEHIECYE*RVK*LQEKNA*FFIVTPNNQL DNIAFSKWRVI*SGCVAFWNLHVTGILKQLIKY WIHFIEMPR*TQCEYHFHF*AVE*HSQILWCML CSFHLKHLSKFCSQT*GSRL*I*GHSRGKQM*FP QCEDKCRIVVILAVKLACSRDWLKKERSNENKM HSLNGRSTVMWHLENL*LS*CYFTCPYALF*SP RDNWMKHYVCSHLI*DGGGNELKYDRELYADFKI *SVLASIKGFSEI*TAW*CILIL*A*NYLEGEM TSFLLLSLFSKASFPQMYVSRTFYSFKRKKTET WHHLGRTKVEFNFLDFKSPG*IMLITVPFELHVL TSFFFLIIPSKISGELHRNFWKWDFVQVTTLHF R*CSCHSCRLPHAAGNPFRNELTVSSVAGEPVT SLPRPQSTPRLVFLES*VLIIKASMQLLHVVPLL QARWILVLTKEMGVHLRARSTQDAQTAAQVSCA SCETSWGKYTN*YIF*CLRHLHCCFSHILLLIT CSKSFVWASSTVFHLHLPDLS*VILFYC*RCQA* KVIKFLFGC*TLM*PLLHTLQVMPSGYYRISAD LLVMSRSNKKVSLWS*E**FQCIGIICLNVFIL ILTPA*IYFCYQMIAIFICSIFLCIVLICYSVLV FIFLCWKPASCKYLQIMTV*SANHFQRLWGMQ* SEVANLFYF*LFYPEGTFLMDICKSST*PLKII LF*SHPSIHTSASPKSSY*FAYPPHVAS*M*INN MEK

Sequence NM_145321

GCTGGTGGCGCCCGCCAGCCGGCCCGGGAGAGGGGACTTGAGCGCTTCGCGGCCGGTGCGCAGCGCGGCTCCGGCCCCGCAGTCTGTCTGCAAGTGGCCA TCCCCGCCCCCGACTCCTGCGGCCGGTGCGGGAGCTGTCAGCTGCACGTTGCGGGGGTACCGGAGAAGCCGGGGTTGCCTTCTCCCGGTTCTGTGCGCTA ACCCCGGAGCCCGGCCCCGGGGAACTAGGGAGCGTCTCCGGCTGCACAGCGCTCAGGTTTTAGGGTTTCCTTTGGGCCACCCAAAATGTTTTTTAAATAC TTTGGATAAGAGCTAAACCACCTGCCACTGGGGACTTCCATACTCTCGGCTCCCTTGTCATCTGATGGCATTTTGATTGTCTCGAGGCCAAGTGACTTGC TGTCCATGATGAGTGATGAGAAGAACCTTGGTGTGTCCCAAAAATTGGTATCACCTTCAAGGAGCACAAGTAGCTGCTCTTCCAAGCAAGGAAGTCGACA GGACAGCTGGGAAGTGGTGGAAGGACTGAGGGGGGAGATGAATTACACCCAGGAGCCACCAGTTCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCC TTAAAAGGCTGGCATAAGAGATTCTTCTATCTGGACAAAGGAATCTTGAAATATGCCAAGAGCCAAACCGATATAGAGAGAGAGAAGCTGCATGGCTGCA TTGATGTCGGGCTCTCAGTGATGTCTGTAAAGAAGTCATCAAAATGCATAGACCTTGACACCGAGGAGCACATCTACCATCTGAAGGTCAAGTCAGAAGA AGTCTTTGATGAGTGGGTATCGAAACTTCGCCACCACAGAATGTATCGTCAGAATGAAATTGCCATGTTTCCACATGAAGTTAACCACTTTTTCTCAGGG TCCACCATCACAGACTCTTCATCTGGGGTGTTTGACTCCATTTCAAGTAGGAAGCGTAGCAGTATATCAAAGCAGAATTTATTTCAAACTGGAAGCAATG TATCATTTTCTTGTGGTGGTGAGACACGAGTTCCATTATGGTTACAGTCTTCAGAGGACATGGAAAAATGCTCCAAAGACCTGGCGCACTGTCATGCCTA CCTGGTAGAAATGAGCCAGCTCCTGCAAAGCATGGACGTCCTGCATCGGACATACTCGGCACCAGCTATCAACGCCATCCAGGGTGGATCTTTTGAAAGT CCCAAAAAGGAAAAAAGATCGCACAGGAGGTGGCGGTCCAGAGCTATTGGCAAAGATGCTAAAGGAACACTGCAGGTCCCGAAACCTTTTTCTGGCCCAG TAAGACTACACTCCTCCAATCCTAATTTGTCAACACTAGATTTTGGAGAAGAGAAAAATTATTCTGATGGCTCTGAAACCTCATCAGAGTTTTCTAAAAT GCAAGAAGATCTGTGTCATATTGCCCATAAAGTTTACTTCACTTTAAGGTCAGCTTTTAATATCATGTCAGCGGAGAGAGAGAAACTGAAGCAGCTGATG GAGCAGGATGCCTCCTCCTCCCCGTCTGCTCAGGTCATTGGTCTGAAGAACGCCCTGTCATCCGAAAACTCCAGAGATGAAAACCGAGCTCTAGTTCATC AGCTTTCTAATGAAAGTAGACTCTCCATCACTGACTCCCTTTCTGAGTTTTTTGATGCTCAGGAAGTTCTGTTATCTCCAAGCTCTTCAGAAAACGAGAT TTCTGATGATGACTCATATGTCAGTGACATAAGTGATAATCTTTCCTTAGATAATCTCAGTAATGATTTAGATAATGAGAGACAGACCTTGGGGCCTGTC CTTGATAGTGGTCGGGAAGCGAAGTCCCGGAGAAGAACGTGCCTGCCGGCGCCCTGCCCGAGCAGCAGTAACATCAGCCTGTGGAACATCCTGAGGAACA ACATCGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGAGGCTCTGCGAGGAGCTGGAGTACAGCGAGCT CCTGGACAAGGCCGCGCAGATTCCCAGCCCCCTGGAAAGGATGGTATATGTGGCAGCCTTTGCCATATCAGCGTATGCATCTAGCTACTACCGAGCTGGA AGCAAGCCATTTAATCCGGTTCTTGGAGAAACATATGAATGTATTCGGGAGGACAAGGGCTTCCAGTTTTTTTCAGAACAGGTCAGCCACCATCCGCCTA TCTCTGCGTGTCATGCTGAGTCTAGAAATTTTGTTTTCTGGCAAGATGTGAGATGGAAAAACAAATTCTGGGGCAAATCCATGGAAATTGTTCCAATTGG CACAACCCATGTGACTCTGCCAGTTTTTGGGGATCATTTTGAGTGGAACAAAGTGACCTCTTGCATCCATAACATCTTAAGCGGGCAGAGGTGGATTGAG CACTATGGAGAGATTGTCATCAAGAACCTGCATGATGATTCCTGCTACTGCAAAGTGAATTTTATAAAGGCAAAATACTGGAGCACTAATGCCCATGAGA TTGAAGGCACAGTGTTTGACAGGAGTGGAAAAGCGGTTCATCGGCTGTTTGGGAAATGGCATGAAAGCATCTACTGTGGCGGCGGCTCCTCTTCTGCCTG TGTATGGAGAGCAAATCCTATGCCGAAAGGCTACGAGCAATACTATAGCTTCACACAGTTTGCGCTGGAATTAAATGAAATGGATCCATCATCAAAGTCT TTATTGCCACCTACTGACACTCGATTTAGGCCAGACCAGAGGTTTCTAGAGGAAGGGAACTTAGAAGAAGCTGAAATACAAAAGCAGAGGATTGAACAAC TGCAGAGAGAAAGGCGGCGGGTCTTAGAAGAAAATCATGTGGAGCACCAGCCTCGGTTTTTCAGGAAATCCGACGATGACTCTTGGGTGAGCAACGGCAC CTATTTGGAACTTAGAAAAGATCTTGGTTTTTCCAAACTGGACCATCCTGTCTTATGGTGAAAAAGTAAAGAAGAAAGATAACATTAGTGTATTTCTCCT GTGCTTGCCTTCTGAAGTGGCACAAACCTGTGTTTATATATTTAAAAGATACTCTAGGATGATCACTTGTGCTTAGCTTAGCATTGTAACTCTTTAAGTC TATATTTTCCTCAGTGCGTTTCTTTACAATTTCAAATGTTACCCTGATTGTTTATATGAATGTAGAACACCTTGACATTTCTTTTTATATATAAACTATT TAATAAAAATGAAAGATTGAATGTTCATGTGTGGGTTAAAAAAAGAAGCTTTAACACTAATTTTCCAAAGGTTAGGGAAGATTCCAATTAAATTTATGCC TTATAAAATTATGTTGTAGAAAAAAAATCAACCTCTCCCAGGTGCATTAAGAAATAAGAATTCCCAGGGTTACTCACCCATGCGTAAGCTACCCAAGTTT AATTTGGTAGCTGAAATATCTTTTTGCCTCAGACAGCTCTTGAATTGCTCATACAGAACAATTCTGCTGGTGCTGGAGTCTGAAGAATATTTTCATTTGC ATTTTAGTGGTTAGGGAGAGGATATAAGATTAATGGAATGTATATTTTTATATAAGACGTATACGGCACCTTCTTGAAAAGGAAGCATTTGAACTTGTTC CTCCCTATAGTTCTATTGCCTTATATGCAAAATTGTACCCTGTTGCTCAGAGAAATTATTCATAAGAGAAAAGAATTCCAATTAATTAAATATCACAATA GCATCCCAGAGAGACAGTAGGAAATTTCTCCTTAGTGAGAGCTGAGTCCTTGAGAAGTTAAGAGACTGTTTCCTGCTTCCCACCCCACCCCGTTTTTTGT CCCTCCGTTTGATCACCTCCCTGTTGAGTATGGAATGTCCCAGTTTAATATAAAACATACAGTTTATTCCACACAGCAGTTTGACTTTGTAAAAGTTTAG CAAAATAACTGATTGTTTTGGATAACTCAACATGTTTTTCTTAAATGCTGTTTTAGTATTTTCTACCTCTTAATAAGCACCATATTTTAGATCTTGTATA AAAAGTTGCCATCTGCCTTATAGACAAATGTAGAGAATTGATGTTCTTGCTTTGTGTTGTGTTCTGGTAAAGCTTTAAGTGAGTGTCTTACCCCTTTCCT ATACTTTCTTGTTATCCATATCATTCTTTTGTGAGTTTTGCAGCAGTCTTGAATAATACAGATTATAACTACTGAAAGGGGAACCTTCGTCTTTTTAAAT GTGTTATTTCACATTACAATAATATGTGTAGTAGTTGAATTGTGTTATCAAAGCATGTCCAACTTCTGTCCAGATGACATTAAAACCAAAGCCTAATTGT TAAGCCTCTTATTCTAAAGTCCATAGCAATGCTATGGAATATCAAGTATAATGATGTAGTAAAAAGATTTCTCCAGAAAACACTTTGAGATACTTAAAGA AATTCAGAGCATATCGAATGTTATGAATGAAGAGTAAAATAATTACAGGAAAAGAATGCATGATTTTTCATTGTCACACCAAATAACCAGTTGGACAATA TTGCATTTTCAAAATGGAGAGTTATTTAAAGTGGATGTGTAGCCTTCTGGAATCTGCACGTGACTGGCATTTTAAAACAACTCATTAAATATTGGATTCA TTTTATCGAGATGCCAAGATGAACACAATGTGAATATCATTTCCATTTCTAAGCAGTTGAATGACACAGTCAGATTCTTTGGTGTATGCTTTGTTCTTTC CATCTCAAGCATTTATCCAAATTCTGCTCACAGACATGAGGAAGCAGGCTGTAGATTTAAGGGCACTCAAGGGGAAAACAAATGTAGTTTCCACAGTGTG AGGATAAATGTAGAATCGTGGTAATATTGGCTGTTAAATTGGCCTGCTCCAGAGACTGGCTCAAAAAAGAAAGAAGCAACGAAAACAAAATGCACAGCCT GAATGGACGTAGCACTGTCATGTGGCATTTGGAAAATCTTTAATTATCCTAATGTTATTTCACTTGCCCTTATGCTTTATTTTAGAGTCCCAGGGACAAC TGGATGAAACATTACGTCTGTAGTCATCTTATTTGAGATGGGGGTGGGAACGAATTAAAATATGATAGAGAACTATATGCAGATTTTAAAATCTAGTCAG TACTAGCATCTATAAAGGGCTTTTCTGAAATTTAAACAGCTTGGTGATGCATCCTGATATTATAAGCTTAAAACTATCTTGAGGGGGAAATGACTTCTTT TCTCCTTCTGTCCCTTTTCTCAAAAGCATCTTTCCCCCAAATGTATGTCTCTAGGACATTTTATAGTTTTAAGAGGAAGAAAACAGAAACGTGGCATCAT TTGGGAAGAACCAAGGTAGAATTTAATTTTCTGGACTTTAAATCTCCTGGTTAAATAATGCTAATCACTGTACCATTTGAGTTACATGTTTTAACCTCTT TTTTTTTTCTAATTATTCCATCCAAAATTTCTGGTGAATTACACAGAAATTTTTGGAAATGGGACTTTGTGCAGGTAACCACCCTGCACTTCAGGTAATG TTCCTGTCACAGCTGTCGACTACCCCACGCTGCAGGAAATCCATTCAGAAATGAGCTAACAGTCTCCAGTGTAGCAGGAGAGCCAGTCACTTCCCTCCCC AGGCCCCAAAGCACCCCAAGGCTGGTATTCCTTGAGTCATAGGTATTAATAATAAAAGCCTCAATGCAGCTTCTCCATGTAGTTCCTCTCCTACAAGCCA GGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACCTGAGGGCAAGAAGTACCCAGGATGCCCAAACAGCCGCACAGGTGTCCTGTGCTTCCTGTGA GACTTCCTGGGGGAAATATACAAACTAATATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCATATTCTGCTTTTAATTACTTGTAGTAAA TCATTTGTTTGGGCTTCAAGCACTGTCTTCCATCTGCATCTTCCAGATTTATCATGAGTGATCTTGTTCTACTGTTAAAGATGTCAAGCCTAAAAAGTAA TAAAGTTTTTATTTGGCTGTTGAACCTTGATGTAGCCCCTACTACATACACTACAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGACCTTTTAGT TATGAGTAGAAGCAATAAGAAGGTGTCCTTATGGTCTTAGGAATAATAGTTCCAATGTATTGGCATAATTTGTTTGAATGTCTTTATTTTGATATTAACA CCGGCATAAATCTATTTTTGCTACCAGATGATAGCTATTTTCATTTGCTCTATTTTCCTCTGCATTGTACTAATCTGTTACTCAGTGTTAGTCTTCATTT TTCTGTGTTGGAAACCTGCGTCATGTAAATATCTGCAAATCATGACAGTCTAAAGTGCAAACCATTTTCAGAGACTTTGGGGAATGCAGTGATCAGAAGT GGCTAATTTATTTTATTTCTGATTATTTTATCCAGAGGGTACTTTTTTAATGGATATTTGTAAATCTTCCACTTAACCACTGAAAATTATTTTGTTTTAA TCCCACCCTTCCATCCATACCTCTGCCTCCCCAAAAAGCTCCTATTAATTTGCTTATCCCCCTCATGTAGCTAGTTGAATGTGAATAAATAACATGGAAA AAAA

refseq_OSBPL3.F2 (forward primer)
refseq_OSBPL3.R2 (reverse primer)
Single exon skipping

Frame 0

AGGARQPARERGLERFAAGAQRGSGPAVCLQVA IPAPDSCGRCGSCQLHVAGVPEKPGLPSPGSVR* PRSPAPGN*GASPAAQRSGFRVSFGPPKMFFKY FG*ELNHLPLGTSILSAPLSSDGILIVSRPSDL LSMMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQ DSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWP LKGWHKRFFYLDKGILKYAKSQTDIEREKLHGC IDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEE VFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSG STITDSSSGVFDSISSRKRSSISKQNLFQTGSN VSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAY LVEMSQLLQSMDVLHRTYSAPAINAIQGGSFES PKKEKRSHRRWRSRAIGKDAKGTLQVPKPFSGP VRLHSSNPNLSTLDFGEEKNYSDGSETSSEFSKM QEDLCHIAHKVYFTLRSAFNIMSAEREKLKQLM EQDASSSPSAQVIGLKNALSSENSRDENRALVH QLSNESRLSITDSLSEFFDAQEVLLSPSSSENEI SDDDSYVSDISDNLSLDNLSNDLDNERQTLGPV LDSGREAKSRRRTCLPAPCPSSSNISLWNILRN NIGKDLSKVAMPVELNEPLNTLQRLCEELEYSEL LDKAAQIPSPLERMVYVAAFAISAYASSYYRAG SKPFNPVLGETYECIREDKGFQFFSEQVSHHPP ISACHAESRNFVFWQDVRWKNKFWGKSMEIVPIG TTHVTLPVFGDHFEWNKVTSCIHNILSGQRWIE HYGEIVIKNLHDDSCYCKVNFIKAKYWSTNAHE IEGTVFDRSGKAVHRLFGKWHESIYCGGGSSSAC VWRANPMPKGYEQYYSFTQFALELNEMDPSSKS LLPPTDTRFRPDQRFLEEGNLEEAEIQKQRIEQ LQRERRRVLEENHVEHQPRFFRKSDDDSWVSNGT YLELRKDLGFSKLDHPVLW*KSKEER*H*CISP VLAF*SGTNLCLYI*KIL*DDHLCLA*HCNSLS LYFPQCVSLQFQMLP*LFI*M*NTLTFLFIYKLF NKNERLNVHVWVKKRSFNTNFPKVREDSN*IYA L*NYVVEKKSTSPRCIKK*EFPGLLTHA*ATQV *FGS*NIFLPQTALELLIQNNSAGAGV*RIFSFA F*WLGRGYKINGMYIFI*DVYGTFLKRKHLNLF LPIVLLPYMQNCTLLLREIIHKRKEFQLIKYHN SIPERQ*EISP**ELSP*EVKRLFPASHPTPFFV PPFDHLPVEYGMSQFNIKHTVYSTQQFDFVKV* QNN*LFWITQHVFLKCCFSIFYLLISTIF*ILY KKLPSAL*TNVEN*CSCFVLCSGKALSECLTPFL YFLVIHIILL*VLQQS*IIQIITTERGTFVFLN VLFHITIICVVVELCYQSMSNFCPDDIKTKA*L LSLLF*SP*QCYGISSIMM**KDFSRKHFEILKE IQSISNVMNEE*NNYRKRMHDFSLSHQITSWTI LHFQNGELFKVDV*PSGICT*LAF*NNSLNIGF ILSRCQDEHNVNIISISKQLNDTVRFFGVCFVLS ISSIYPNSAHRHEEAGCRFKGTQGENKCSFHSV RINVESW*YWLLNWPAPETGSKKKEATKTKCTA *MDVALSCGIWKIFNYPNVISLALMLYFRVPGTT G*NITSVVILFEMGVGTN*NMIENYMQILKSSQ Y*HL*RAFLKFKQLGDAS*YYKLKTILRGK*LL FSFCPFSQKHLSPKCMSLGHFIVLRGRKQKRGII WEEPR*NLIFWTLNLLVK*C*SLYHLSYMF*PL FFF*LFHPKFLVNYTEIFGNGTLCR*PPCTSGN VPVTAVDYPTLQEIHSEMS*QSPV*QESQSLPSP GPKAPQGWYSLSHRY***KPQCSFSM*FLSYKP GGFWS*LKRWEFT*GQEVPRMPKQPHRCPVLPV RLPGGNIQTNIFFNVYVIYTAAFLIFCF*LLVVN HLFGLQALSSICIFQIYHE*SCSTVKDVKPKK* *SFYLAVEP*CSPYYIHYKLCLLATTESPQTF* L*VEAIRRCPYGLRNNSSNVLA*FV*MSLF*Y*H RHKSIFATR**LFSFALFSSALY*SVTQC*SSF FCVGNLRHVNICKS*QSKVQTIFRDFGECSDQK WLIYFISDYFIQRVLF*WIFVNLPLNH*KLFCFN PTLPSIPLPPQKAPINLLIPLM*LVECE*ITWK K

Frame 1

LVAPASRPGRGDLSASRPVRSAAPAPQSVCKWP SPPPTPAAGAGAVSCTLRGYRRSRGCLLPVLCA NPGARPRGTRERLRLHSAQVLGFPLGHPKCFLNT LDKS*TTCHWGLPYSRLPCHLMAF*LSRGQVTC CP**VMRRTLVCPKNWYHLQGAQVAALPSKEVD RTAGKWWKD*GGR*ITPRSHQFRKDFC*KRGSGP *KAGIRDSSIWTKES*NMPRAKPI*RERSCMAA LMSGSQ*CL*RSHQNA*TLTPRSTSTI*RSSQK KSLMSGYRNFATTECIVRMKLPCFHMKLTTFSQG PPSQTLHLGCLTPFQVGSVAVYQSRIYFKLEAM YHFLVVVRHEFHYGYSLQRTWKNAPKTWRTVMP TW*K*ASSCKAWTSCIGHTRHQLSTPSRVDLLKV PKRKKDRTGGGGPELLAKMLKEHCRSRNLFLAQ *DYTPPILICQH*ILEKRKIILMALKPHQSFLK CKKICVILPIKFTSL*GQLLISCQRRERN*SS*W SRMPPPPRLLRSLV*RTPCHPKTPEMKTEL*FI SFLMKVDSPSLTPFLSFLMLRKFCYLQALQKTR FLMMTHMSVT*VIIFP*IISVMI*IMRDRPWGLS LIVVGKRSPGEERACRRPARAAVTSACGTS*GT TSGRTCPRWPCRWS*TSP*TRCRGSARSWSTAS SWTRPRRFPAPWKGWYMWQPLPYQRMHLATTELE ASHLIRFLEKHMNVFGRTRASSFFQNRSATIRL SLRVMLSLEILFSGKM*DGKTNSGANPWKLFQL AQPM*LCQFLGIILSGTK*PLASITS*AGRGGLS TMERLSSRTCMMIPATAK*IL*RQNTGALMPMR LKAQCLTGVEKRFIGCLGNGMKASTVAAAPLLP VYGEQILCRKATSNTIASHSLRWN*MKWIHHQSL YCHLLTLDLGQTRGF*RKGT*KKLKYKSRGLNN CREKGGGS*KKIMWSTSLGFSGNPTMTLG*ATA PIWNLEKILVFPNWTILSYGEKVKKKDNISVFLL CLPSEVAQTCVYIFKRYSRMITCA*LSIVTL*V YIFLSAFLYNFKCYPDCLYECRTP*HFFLYINY LIKMKD*MFMCGLKKEALTLIFQRLGKIPIKFMP YKIML*KKNQPLPGALRNKNSQGYSPMRKLPKF NLVAEISFCLRQLLNCSYRTILLVLESEEYFHL HFSG*GEDIRLMECIFLYKTYTAPS*KGSI*TCS SL*FYCLICKIVPCCSEKLFIREKNSN*LNITI ASQRDSRKFLLSES*VLEKLRDCFLLPTPPRFL SLRLITSLLSMECPSLI*NIQFIPHSSLTL*KFS KITDCFG*LNMFFLNAVLVFSTS**APYFRSCI KSCHLPYRQM*RIDVLALCCVLVKL*VSVLPLS YTFLLSISFFCEFCSSLE*YRL*LLKGEPSSF*M CYFTLQ*YV**LNCVIKACPTSVQMTLKPKPNC *ASYSKVHSNAMEYQV**CSKKISPENTLRYLK KFRAYRML*MKSKIITGKECMIFHCHTK*PVGQY CIFKMESYLKWMCSLLESARDWHFKTTH*ILDS FYRDAKMNTM*ISFPFLSS*MTQSDSLVYALFF PSQAFIQILLTDMRKQAVDLRALKGKTNVVSTV* G*M*NRGNIGC*IGLLQRLAQKRKKQRKQNAQP EWT*HCHVAFGKSLIILMLFHLPLCFILESQGQ LDETLRL*SSYLRWGWERIKI**RTICRF*NLVS TSIYKGLF*NLNSLVMHPDIISLKLS*GGNDFF SPSVPFLKSIFPPNVCL*DIL*F*EEENRNVAS FGKNQGRI*FSGL*ISWLNNANHCTI*VTCFNLF FFSNYSIQNFW*ITQKFLEMGLCAGNHPALQVM FLSQLSTTPRCRKSIQK*ANSLQCSRRASHFPP QAPKHPKAGIP*VIGINNKSLNAASPCSSSPTSQ VDSGPN*RDGSSPEGKKYPGCPNSRTGVLCFL* DFLGEIYKLIYFLMFTSFTLLLFSYSAFNYL** IICLGFKHCLPSASSRFIMSDLVLLLKMSSLKSN KVFIWLLNLDVAPTTYTTSYAFWLLQNLRRPFS YE*KQ*EGVLMVLGIIVPMYWHNLFECLYFDIN TGINLFLLPDDSYFHLLYFPLHCTNLLLSVSLHF SVLETCVM*ISANHDSLKCKPFSETLGNAVIRS G*FILFLIILSRGYFFNGYL*IFHLTTENYFVL IPPFHPYLCLPKKLLLICLSPSCS*LNVNK*HGK K

Frame 2

WWRPPAGPGEGT*ALRGRCAARLRPRSLSASGH PRPRLLRPVRELSAARCGGTGEAGVAFSRFCAL TPEPGPGELGSVSGCTALRF*GFLWATQNVF*I LWIRAKPPATGDFHTLGSLVI*WHFDCLEAK*LA VHDE**EEPWCVPKIGITFKEHK*LLFQARKST GQLGSGGRTEGGDELHPGATSSERIFAEKEEVA LKRLA*EILLSGQRNLEICQEPNRYREREAAWLH *CRALSDVCKEVIKMHRP*HRGAHLPSEGQVRR SL**VGIETSPPQNVSSE*NCHVST*S*PLFLR VHHHRLFIWGV*LHFK*EA*QYIKAEFISNWKQC IIFLWW*DTSSIMVTVFRGHGKMLQRPGALSCL PGRNEPAPAKHGRPASDILGTSYQRHPGWIF*K SQKGKKIAQEVAVQSYWQRC*RNTAGPETFFWPS KTTLLQS*FVNTRFWRREKLF*WL*NLIRVF*N ARRSVSYCP*SLLHFKVSF*YHVSGERETEAAD GAGCLLLPVCSGHWSEERPVIRKLQR*KPSSSSS AF**K*TLHH*LPF*VF*CSGSSVISKLFRKRD F***LICQ*HK**SFLR*SQ**FR**ETDLGAC P**WSGSEVPEKNVPAGALPEQQ*HQPVEHPEEQ HREGPVQGGHAGGAERAPEHAAEALRGAGVQRA PGQGRADSQPPGKDGICGSLCHISVCI*LLPSW KQAI*SGSWRNI*MYSGGQGLPVFFRTGQPPSAY LCVSC*V*KFCFLARCEMEKQILGQIHGNCSNW HNPCDSASFWGSF*VEQSDLLHP*HLKRAEVD* ALWRDCHQEPA**FLLLQSEFYKGKILEH*CP*D *RHSV*QEWKSGSSAVWEMA*KHLLWRRLLFCL CMESKSYAERLRAIL*LHTVCAGIK*NGSIIKV FIATY*HSI*ARPEVSRGRELRRS*NTKAED*TT AERKAAGLRRKSCGAPASVFQEIRR*LLGEQRH LFGT*KRSWFFQTGPSCLMVKK*RRKITLVYFS CACLLKWHKPVFIYLKDTLG*SLVLSLAL*LFKS IFSSVRFFTISNVTLIVYMNVEHLDISFYI*TI **K*KIECSCVG*KKKL*H*FSKG*GRFQLNLC LIKLCCRKKINLSQVH*EIRIPRVTHPCVSYPSL IW*LKYLFASDSS*IAHTEQFCWCWSLKNIFIC ILVVRERI*D*WNVYFYIRRIRHLLEKEAFELV PPYSSIALYAKLYPVAQRNYS*EKRIPIN*ISQ* HPRETVGNFSLVRAESLRS*ETVSCFPPHPVFC PSV*SPPC*VWNVPV*YKTYSLFHTAV*LCKSL AK*LIVLDNSTCFS*MLF*YFLPLNKHHILDLV* KVAICLIDKCRELMFLLCVVFW*SFK*VSYPFP ILSCYPYHSFVSFAAVLNNTDYNY*KGNLRLFK CVISHYNNMCSS*IVLSKHVQLLSR*H*NQSLIV KPLILKSIAMLWNIKYNDVVKRFLQKTL*DT*R NSEHIECYE*RVK*LQEKNA*FFIVTPNNQLDN IAFSKWRVI*SGCVAFWNLHVTGILKQLIKYWIH FIEMPR*TQCEYHFHF*AVE*HSQILWCMLCSF HLKHLSKFCSQT*GSRL*I*GHSRGKQM*FPQC EDKCRIVVILAVKLACSRDWLKKERSNENKMHSL NGRSTVMWHLENL*LS*CYFTCPYALF*SPRDN WMKHYVCSHLI*DGGGNELKYDRELYADFKI*S VLASIKGFSEI*TAW*CILIL*A*NYLEGEMTSF LLLSLFSKASFPQMYVSRTFYSFKRKKTETWHH LGRTKVEFNFLDFKSPG*IMLITVPFELHVLTS FFFLIIPSKISGELHRNFWKWDFVQVTTLHFR*C SCHSCRLPHAAGNPFRNELTVSSVAGEPVTSLP RPQSTPRLVFLES*VLIIKASMQLLHVVPLLQA RWILVLTKEMGVHLRARSTQDAQTAAQVSCASCE TSWGKYTN*YIF*CLRHLHCCFSHILLLITCSK SFVWASSTVFHLHLPDLS*VILFYC*RCQA*KV IKFLFGC*TLM*PLLHTLQVMPSGYYRISADLLV MSRSNKKVSLWS*E**FQCIGIICLNVFILILT PA*IYFCYQMIAIFICSIFLCIVLICYSVLVFI FLCWKPASCKYLQIMTV*SANHFQRLWGMQ*SEV ANLFYF*LFYPEGTFLMDICKSST*PLKIILF* SHPSIHTSASPKSSY*FAYPPHVAS*M*INNME K