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Sequences

Sequence NM_001017957

GAGGCCGCTGCCTGGCTTAGGGCGGAAACAGATTCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCT GCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCG TTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTC ACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGAC AAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTAC TACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGT GCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTT GTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCAC CCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCC CCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGAT GCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCC AATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGC CAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGA AGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCA GACCGAGACCGGCTCCGTTCGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCA CTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATC ACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAAC CTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGT GGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGAGACCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGA CTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTG GGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCC TCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATAGGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGA GAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTG CCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACAATTTGTGCTTCTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGGAAATGGTAGA ATGCTGCCTATCACCTCCAGCACAATCCCAGTGAAAAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAAATAAACAACTGGACCATCA A

refseq_OS9.F1 (forward primer)
refseq_OS9.R1 (reverse primer)
Single exon skipping

Frame 0

EAAAWLRAETDSLHKKGNERWRRKRCCPVC*DC CFWDSCYPQV*PAVSGA*TWRS*VRCVMGSRSCR CLSWEGRANLRTW*LSPLSTNSAMSVACQLELF TSSVKGRRKHLLTKGLGSLSC*AQ*EMLPAC*R QRTGGHMNSVMDATSSNTTWKIQRSKVKSSISAT TNQPSTGMMKQPRPPSSIVLNATTARPMAMGPS ATLMGGPGRPRFGSSVTRVQVSLGTTSIAWTSP CPALMC*PFALLGSAPTLSSGPHPVLHRRPSSVT LPYSLRSTWPTFRGKPTQSSMEIKS*RSCKI*A PKCGVRPSLGWHPKRWQVRARPRMTVRTQISGR CLMSQRTRPQEGRRCRLRSRTQALRQQIQLLVLP MIFRTTCRSKSFEALRI*FDS*RS*KVEQKRGS QI*AKSSLWMMLQKSLRGNQRRKGVIQNGRERW KKRRMRMRMRMKMRMNGSYWENLRRNWKGSCFRQ TETGSVRRQRKSWTQMG*RRSQSGIGQCWLSHP LSTNSSKDWRKNRVQSW*RSTRKRGLSPKSLPH HPNLQGKLRSKLSAHGLKGLKRVHVG*LMRTRET SRRSSSISWCRELKRPRRNASGRKSWRAITAGC GALQVGRAQGTWTNLTSETNTTLDPSRNPDSSW TGLPPPHLPTLEPLRAKQQSGAELWTSRATLWVW GPWVNAAAPAGSHLETSPGL*YLLS*TLTQSSS SPLWLFLLLSPNDRIFPAAYLEIQ*DLLSGGG* REQGGQDT*QALSKQAPTCP**CLESFYPLLLNC LGISFSLSLPTLSPTICASELRSLHLCC*GNGR MLPITSSTIPVKKV*STHHVLEQSGF*INNWTI

Frame 1

RPLPGLGRKQILCIRRGTKDGGGNAAVQFVRTA ASGTPVTRKSDRRCREPEPGGAE*DALWDRDPA VACHGRAEPIFGRGDCLL*VQTAL*VSPASWSYS LPA*KGGGNTCLPRAWDP*VVEPNERCSLLAED KGLVDI*ILLWTPHPAIPHGRFRDQR*SPLSRL LPISLRLG**NSQGLQAASS*TLPQPDLWQWVQV RP*WEAPGGRGSVPL*RGCRYLWGLHRSRGRAL VLLLCADHSHSSALPPPSPPAPTQCCTAGHPLS PFPTA*GVHGLRSEASRLKAVWR*NHRGAARSRP PSVE*DQVWGGTPKDGRCEPDQG*Q*GLRFLED A**ARGPGPRRGGGAG*GAGPKP*GSRFSFWCS Q*FSEQRAGQSHSKPCGFDSIHRGAERWNKKGEA KYRPRAACG*CCRSPSEGTREGKG*SRTAERDG RRGG*G*G*G*R*G*TAVTGRI*EGTGRDPASV RPRPAPFGDRERAGPRWAEEGVRAGSGNAGSHIH SQQTHQKTGGKTESRAGEEAQEKEGCPQKASPI TPTYREN*DQNCPPMG*RD*RGCTLAD**GHEK PQGDLLQYLGAGS*RGPEGTPAAERAGEQLPPGV GLSRWGGHRGPGRI*LLRPTLHLTLHGIQTLPG LACLLPTSPPWNP*GPNSRVELSCGPLGQLCGC GGPG*MLLPLLAATLRPHRACDICSPELSLNPLP LLCGFSCYCPLMIGYSLLPTWRFSRIF*VEVGR ESKEGRTLSRH*ASRPPPALSDVWSRFTLFY*I ALGFPSPFPCPPCPLQFVLLS*GAFTSVAEEMVE CCLSPPAQSQ*KRCEAPTMFLNNQVSK*TTGPS

Frame 2

GRCLA*GGNRFSA*EGERKMAAETLLSSLLGLL LLGLLLPASLTGGVGSLNLEELSEMRYGIEILP LPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAI HFQREREEETPAYQGPGIPELLSPMRDAPCLLKT KDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGY YQSAFDWDDETAKASKQHRLKRYHSQTYGNGSK CDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPL SCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCH PSLQPEEYMAYVQRQADSKQYGDKIIEELQDLG PQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKM LNEPEDQAPGGEEVPAEEQDPSPEAADSASGAP NDFQNNVQVKVIRSPADLIRFIEELKGGTKKGK PNIGQEQPVDDAAEVPQREPEKERGDPERQREME EEEDEDEDEDEDEDERQLLGEFEKELEGILLPS DRDRLRSETEKELDPDGLKKESERDRAMLALTS TLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPS PQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRN LKEIFFNILVPGAEEAQKERQRQKELESNYRRV WGSPGGEGTGDLDEFDF*DQHYT*PFTESRLFLD WLASSPPPHPGTPEGQTAEWS*AVDLSGNSVGV GALGECCCPCWQPP*DLTGPVIFALLNSHSILF LSSVAFPVIVP***DIPCCLPGDSVGSFEWRWVE RARRAGHLAGTEQAGPHLPLVMFGVVLPSSIEL PWDFLLPFPAHPVPYNLCF*VEEPSPLLLRKW* NAAYHLQHNPSEKGVKHPPCS*TIRFLNKQLDHQ

Sequence NM_001017956

GAGGCCGCTGCCTGGCTTAGGGCGGAAACAGATTCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCT GCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCG TTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTC ACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGAC AAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTAC TACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGT GCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTT GTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCAC CCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCC CCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGAT GCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCC AATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGC CAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGA AGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCA GACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGA AGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAA GAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGG ACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGG AACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGAGA CCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCA GAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCG GGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATAGGATATTCCCTGCTG CCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTG CCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACAATTTGTGCT TCTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGGAAATGGTAGAATGCTGCCTATCACCTCCAGCACAATCCCAGTGAAAAAGGTGTGAAGCACCCACC ATGTTCTTGAACAATCAGGTTTCTAAATAAACAACTGGACCATCAA

refseq_OS9.F1 (forward primer)
refseq_OS9.R1 (reverse primer)
Single exon skipping

Frame 0

EAAAWLRAETDSLHKKGNERWRRKRCCPVC*DC CFWDSCYPQV*PAVSGA*TWRS*VRCVMGSRSCR CLSWEGRANLRTW*LSPLSTNSAMSVACQLELF TSSVKGRRKHLLTKGLGSLSC*AQ*EMLPAC*R QRTGGHMNSVMDATSSNTTWKIQRSKVKSSISAT TNQPSTGMMKQPRPPSSIVLNATTARPMAMGPS ATLMGGPGRPRFGSSVTRVQVSLGTTSIAWTSP CPALMC*PFALLGSAPTLSSGPHPVLHRRPSSVT LPYSLRSTWPTFRGKPTQSSMEIKS*RSCKI*A PKCGVRPSLGWHPKRWQVRARPRMTVRTQISGR CLMSQRTRPQEGRRCRLRSRTQALRQQIQLLVLP MIFRTTCRSKSFEALRI*FDS*RS*KVEQKRGS QI*AKSSLWMMLQKSLRGNQRRKGVIQNGRERW KKRRMRMRMRMKMRMNGSYWENLRRNWKGSCFRQ TETGSVRR*RLAWSGNWKTSSRRQRKSWTQMG* RRSQSGIGQCWLSHPLSTNSSKDWRKNRVQSW* RSTRKRGLSPKSLPHHPNLQGKLRSKLSAHGLKG LKRVHVG*LMRTRETSRRSSSISWCRELKRPRR NASGRKSWRAITAGCGALQVGRAQGTWTNLTSE TNTTLDPSRNPDSSWTGLPPPHLPTLEPLRAKQQ SGAELWTSRATLWVWGPWVNAAAPAGSHLETSP GL*YLLS*TLTQSSSSPLWLFLLLSPNDRIFPA AYLEIQ*DLLSGGG*REQGGQDT*QALSKQAPTC P**CLESFYPLLLNCLGISFSLSLPTLSPTICA SELRSLHLCC*GNGRMLPITSSTIPVKKV*STH HVLEQSGF*INNWTI

Frame 1

RPLPGLGRKQILCIRRGTKDGGGNAAVQFVRTA ASGTPVTRKSDRRCREPEPGGAE*DALWDRDPA VACHGRAEPIFGRGDCLL*VQTAL*VSPASWSYS LPA*KGGGNTCLPRAWDP*VVEPNERCSLLAED KGLVDI*ILLWTPHPAIPHGRFRDQR*SPLSRL LPISLRLG**NSQGLQAASS*TLPQPDLWQWVQV RP*WEAPGGRGSVPL*RGCRYLWGLHRSRGRAL VLLLCADHSHSSALPPPSPPAPTQCCTAGHPLS PFPTA*GVHGLRSEASRLKAVWR*NHRGAARSRP PSVE*DQVWGGTPKDGRCEPDQG*Q*GLRFLED A**ARGPGPRRGGGAG*GAGPKP*GSRFSFWCS Q*FSEQRAGQSHSKPCGFDSIHRGAERWNKKGEA KYRPRAACG*CCRSPSEGTREGKG*SRTAERDG RRGG*G*G*G*R*G*TAVTGRI*EGTGRDPASV RPRPAPFGGEGWHGAGTGKHHPGDRERAGPRWAE EGVRAGSGNAGSHIHSQQTHQKTGGKTESRAGE EAQEKEGCPQKASPITPTYREN*DQNCPPMG*R D*RGCTLAD**GHEKPQGDLLQYLGAGS*RGPEG TPAAERAGEQLPPGVGLSRWGGHRGPGRI*LLR PTLHLTLHGIQTLPGLACLLPTSPPWNP*GPNS RVELSCGPLGQLCGCGGPG*MLLPLLAATLRPHR ACDICSPELSLNPLPLLCGFSCYCPLMIGYSLL PTWRFSRIF*VEVGRESKEGRTLSRH*ASRPPP ALSDVWSRFTLFY*IALGFPSPFPCPPCPLQFVL LS*GAFTSVAEEMVECCLSPPAQSQ*KRCEAPT MFLNNQVSK*TTGPS

Frame 2

GRCLA*GGNRFSA*EGERKMAAETLLSSLLGLL LLGLLLPASLTGGVGSLNLEELSEMRYGIEILP LPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAI HFQREREEETPAYQGPGIPELLSPMRDAPCLLKT KDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGY YQSAFDWDDETAKASKQHRLKRYHSQTYGNGSK CDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPL SCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCH PSLQPEEYMAYVQRQADSKQYGDKIIEELQDLG PQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKM LNEPEDQAPGGEEVPAEEQDPSPEAADSASGAP NDFQNNVQVKVIRSPADLIRFIEELKGGTKKGK PNIGQEQPVDDAAEVPQREPEKERGDPERQREME EEEDEDEDEDEDEDERQLLGEFEKELEGILLPS DRDRLRSEVKAGMERELENIIQETEKELDPDGL KKESERDRAMLALTSTLNKLIKRLEEKQSPELVK KHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEG TEEGARWLTDEDTRNLKEIFFNILVPGAEEAQK ERQRQKELESNYRRVWGSPGGEGTGDLDEFDF*D QHYT*PFTESRLFLDWLASSPPPHPGTPEGQTA EWS*AVDLSGNSVGVGALGECCCPCWQPP*DLT GPVIFALLNSHSILFLSSVAFPVIVP***DIPCC LPGDSVGSFEWRWVERARRAGHLAGTEQAGPHL PLVMFGVVLPSSIELPWDFLLPFPAHPVPYNLC F*VEEPSPLLLRKW*NAAYHLQHNPSEKGVKHPP CS*TIRFLNKQLDHQ

Sequence NM_006812

GAGGCCGCTGCCTGGCTTAGGGCGGAAACAGATTCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCT GCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCG TTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTC ACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGAC AAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTAC TACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGT GCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTT GTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCAC CCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCC CCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGAT GCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCC AATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGC CAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGA AGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCA GACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGA AGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAA GAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAG CTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGG AGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGA CACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTAC CGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGAGACCAACACTACACTTGACCCTTCACGGAATCCAGAC TCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTC TGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCA ATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATAGGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGA GGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTC TATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACAATTTGTGCTTCTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGG AAATGGTAGAATGCTGCCTATCACCTCCAGCACAATCCCAGTGAAAAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAAATAAACAAC TGGACCATCAA

refseq_OS9.F1 (forward primer)
refseq_OS9.R1 (reverse primer)
Single exon skipping

Frame 0

EAAAWLRAETDSLHKKGNERWRRKRCCPVC*DC CFWDSCYPQV*PAVSGA*TWRS*VRCVMGSRSCR CLSWEGRANLRTW*LSPLSTNSAMSVACQLELF TSSVKGRRKHLLTKGLGSLSC*AQ*EMLPAC*R QRTGGHMNSVMDATSSNTTWKIQRSKVKSSISAT TNQPSTGMMKQPRPPSSIVLNATTARPMAMGPS ATLMGGPGRPRFGSSVTRVQVSLGTTSIAWTSP CPALMC*PFALLGSAPTLSSGPHPVLHRRPSSVT LPYSLRSTWPTFRGKPTQSSMEIKS*RSCKI*A PKCGVRPSLGWHPKRWQVRARPRMTVRTQISGR CLMSQRTRPQEGRRCRLRSRTQALRQQIQLLVLP MIFRTTCRSKSFEALRI*FDS*RS*KVEQKRGS QI*AKSSLWMMLQKSLRGNQRRKGVIQNGRERW KKRRMRMRMRMKMRMNGSYWENLRRNWKGSCFRQ TETGSVRR*RLAWSGNWKTSSRRQRKSWTQMG* RRSQSGIGQCWLSHPLSTNSSKDWRKNRVQSW* RSTRKRGLSPKSLPHHPNLQRRILSTESGSGSPS SVSEALIRI*LSSR*NGKTHS*NKSRGW*RSCW RGRDSQLQGKLRSKLSAHGLKGLKRVHVG*LMR TRETSRRSSSISWCRELKRPRRNASGRKSWRAIT AGCGALQVGRAQGTWTNLTSETNTTLDPSRNPD SSWTGLPPPHLPTLEPLRAKQQSGAELWTSRAT LWVWGPWVNAAAPAGSHLETSPGL*YLLS*TLTQ SSSSPLWLFLLLSPNDRIFPAAYLEIQ*DLLSG GG*REQGGQDT*QALSKQAPTCP**CLESFYPL LLNCLGISFSLSLPTLSPTICASELRSLHLCC*G NGRMLPITSSTIPVKKV*STHHVLEQSGF*INN WTI

Frame 1

RPLPGLGRKQILCIRRGTKDGGGNAAVQFVRTA ASGTPVTRKSDRRCREPEPGGAE*DALWDRDPA VACHGRAEPIFGRGDCLL*VQTAL*VSPASWSYS LPA*KGGGNTCLPRAWDP*VVEPNERCSLLAED KGLVDI*ILLWTPHPAIPHGRFRDQR*SPLSRL LPISLRLG**NSQGLQAASS*TLPQPDLWQWVQV RP*WEAPGGRGSVPL*RGCRYLWGLHRSRGRAL VLLLCADHSHSSALPPPSPPAPTQCCTAGHPLS PFPTA*GVHGLRSEASRLKAVWR*NHRGAARSRP PSVE*DQVWGGTPKDGRCEPDQG*Q*GLRFLED A**ARGPGPRRGGGAG*GAGPKP*GSRFSFWCS Q*FSEQRAGQSHSKPCGFDSIHRGAERWNKKGEA KYRPRAACG*CCRSPSEGTREGKG*SRTAERDG RRGG*G*G*G*R*G*TAVTGRI*EGTGRDPASV RPRPAPFGGEGWHGAGTGKHHPGDRERAGPRWAE EGVRAGSGNAGSHIHSQQTHQKTGGKTESRAGE EAQEKEGCPQKASPITPTYRGGS*AQSPGPGHQ APSRRP*SGSDCPRDETGKPTAETNRGAGEGAAG EGGTHSCREN*DQNCPPMG*RD*RGCTLAD**G HEKPQGDLLQYLGAGS*RGPEGTPAAERAGEQL PPGVGLSRWGGHRGPGRI*LLRPTLHLTLHGIQT LPGLACLLPTSPPWNP*GPNSRVELSCGPLGQL CGCGGPG*MLLPLLAATLRPHRACDICSPELSL NPLPLLCGFSCYCPLMIGYSLLPTWRFSRIF*VE VGRESKEGRTLSRH*ASRPPPALSDVWSRFTLF Y*IALGFPSPFPCPPCPLQFVLLS*GAFTSVAE EMVECCLSPPAQSQ*KRCEAPTMFLNNQVSK*TT GPS

Frame 2

GRCLA*GGNRFSA*EGERKMAAETLLSSLLGLL LLGLLLPASLTGGVGSLNLEELSEMRYGIEILP LPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAI HFQREREEETPAYQGPGIPELLSPMRDAPCLLKT KDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGY YQSAFDWDDETAKASKQHRLKRYHSQTYGNGSK CDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPL SCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCH PSLQPEEYMAYVQRQADSKQYGDKIIEELQDLG PQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKM LNEPEDQAPGGEEVPAEEQDPSPEAADSASGAP NDFQNNVQVKVIRSPADLIRFIEELKGGTKKGK PNIGQEQPVDDAAEVPQREPEKERGDPERQREME EEEDEDEDEDEDEDERQLLGEFEKELEGILLPS DRDRLRSEVKAGMERELENIIQETEKELDPDGL KKESERDRAMLALTSTLNKLIKRLEEKQSPELVK KHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTK LRLGGPNQDLTVLEMKRENPQLKQIEGLVKELL EREGLTAAGKIEIKIVRPWAEGTEEGARWLTDED TRNLKEIFFNILVPGAEEAQKERQRQKELESNY RRVWGSPGGEGTGDLDEFDF*DQHYT*PFTESR LFLDWLASSPPPHPGTPEGQTAEWS*AVDLSGNS VGVGALGECCCPCWQPP*DLTGPVIFALLNSHS ILFLSSVAFPVIVP***DIPCCLPGDSVGSFEW RWVERARRAGHLAGTEQAGPHLPLVMFGVVLPSS IELPWDFLLPFPAHPVPYNLCF*VEEPSPLLLR KW*NAAYHLQHNPSEKGVKHPPCS*TIRFLNKQ LDHQ

Sequence NM_001017958

GAGGCCGCTGCCTGGCTTAGGGCGGAAACAGATTCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCT GCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCG TTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTC ACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGAC AAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTAC TACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGT GCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTT GTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCAC CCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCC CCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGAT GCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCC AATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGC CAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGA AGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCA GACCGAGACCGGCTCCGTTCGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCA CTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATC ACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATG AAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTG TCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGG AGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTG GACGAATTTGACTTCTGAGACCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAA CCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCA GCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAA TGATAGGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTG AGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCC TGTCCCCTACAATTTGTGCTTCTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGGAAATGGTAGAATGCTGCCTATCACCTCCAGCACAATCCCAGTGAA AAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAAATAAACAACTGGACCATCAA

refseq_OS9.F1 (forward primer)
refseq_OS9.R1 (reverse primer)
Single exon skipping

Frame 0

EAAAWLRAETDSLHKKGNERWRRKRCCPVC*DC CFWDSCYPQV*PAVSGA*TWRS*VRCVMGSRSCR CLSWEGRANLRTW*LSPLSTNSAMSVACQLELF TSSVKGRRKHLLTKGLGSLSC*AQ*EMLPAC*R QRTGGHMNSVMDATSSNTTWKIQRSKVKSSISAT TNQPSTGMMKQPRPPSSIVLNATTARPMAMGPS ATLMGGPGRPRFGSSVTRVQVSLGTTSIAWTSP CPALMC*PFALLGSAPTLSSGPHPVLHRRPSSVT LPYSLRSTWPTFRGKPTQSSMEIKS*RSCKI*A PKCGVRPSLGWHPKRWQVRARPRMTVRTQISGR CLMSQRTRPQEGRRCRLRSRTQALRQQIQLLVLP MIFRTTCRSKSFEALRI*FDS*RS*KVEQKRGS QI*AKSSLWMMLQKSLRGNQRRKGVIQNGRERW KKRRMRMRMRMKMRMNGSYWENLRRNWKGSCFRQ TETGSVRRQRKSWTQMG*RRSQSGIGQCWLSHP LSTNSSKDWRKNRVQSW*RSTRKRGLSPKSLPH HPNLQRRILSTESGSGSPSSVSEALIRI*LSSR* NGKTHS*NKSRGW*RSCWRGRDSQLQGKLRSKL SAHGLKGLKRVHVG*LMRTRETSRRSSSISWCR ELKRPRRNASGRKSWRAITAGCGALQVGRAQGTW TNLTSETNTTLDPSRNPDSSWTGLPPPHLPTLE PLRAKQQSGAELWTSRATLWVWGPWVNAAAPAG SHLETSPGL*YLLS*TLTQSSSSPLWLFLLLSPN DRIFPAAYLEIQ*DLLSGGG*REQGGQDT*QAL SKQAPTCP**CLESFYPLLLNCLGISFSLSLPT LSPTICASELRSLHLCC*GNGRMLPITSSTIPVK KV*STHHVLEQSGF*INNWTI

Frame 1

RPLPGLGRKQILCIRRGTKDGGGNAAVQFVRTA ASGTPVTRKSDRRCREPEPGGAE*DALWDRDPA VACHGRAEPIFGRGDCLL*VQTAL*VSPASWSYS LPA*KGGGNTCLPRAWDP*VVEPNERCSLLAED KGLVDI*ILLWTPHPAIPHGRFRDQR*SPLSRL LPISLRLG**NSQGLQAASS*TLPQPDLWQWVQV RP*WEAPGGRGSVPL*RGCRYLWGLHRSRGRAL VLLLCADHSHSSALPPPSPPAPTQCCTAGHPLS PFPTA*GVHGLRSEASRLKAVWR*NHRGAARSRP PSVE*DQVWGGTPKDGRCEPDQG*Q*GLRFLED A**ARGPGPRRGGGAG*GAGPKP*GSRFSFWCS Q*FSEQRAGQSHSKPCGFDSIHRGAERWNKKGEA KYRPRAACG*CCRSPSEGTREGKG*SRTAERDG RRGG*G*G*G*R*G*TAVTGRI*EGTGRDPASV RPRPAPFGDRERAGPRWAEEGVRAGSGNAGSHIH SQQTHQKTGGKTESRAGEEAQEKEGCPQKASPI TPTYRGGS*AQSPGPGHQAPSRRP*SGSDCPRD ETGKPTAETNRGAGEGAAGEGGTHSCREN*DQNC PPMG*RD*RGCTLAD**GHEKPQGDLLQYLGAG S*RGPEGTPAAERAGEQLPPGVGLSRWGGHRGP GRI*LLRPTLHLTLHGIQTLPGLACLLPTSPPWN P*GPNSRVELSCGPLGQLCGCGGPG*MLLPLLA ATLRPHRACDICSPELSLNPLPLLCGFSCYCPL MIGYSLLPTWRFSRIF*VEVGRESKEGRTLSRH* ASRPPPALSDVWSRFTLFY*IALGFPSPFPCPP CPLQFVLLS*GAFTSVAEEMVECCLSPPAQSQ* KRCEAPTMFLNNQVSK*TTGPS

Frame 2

GRCLA*GGNRFSA*EGERKMAAETLLSSLLGLL LLGLLLPASLTGGVGSLNLEELSEMRYGIEILP LPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAI HFQREREEETPAYQGPGIPELLSPMRDAPCLLKT KDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGY YQSAFDWDDETAKASKQHRLKRYHSQTYGNGSK CDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPL SCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCH PSLQPEEYMAYVQRQADSKQYGDKIIEELQDLG PQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKM LNEPEDQAPGGEEVPAEEQDPSPEAADSASGAP NDFQNNVQVKVIRSPADLIRFIEELKGGTKKGK PNIGQEQPVDDAAEVPQREPEKERGDPERQREME EEEDEDEDEDEDEDERQLLGEFEKELEGILLPS DRDRLRSETEKELDPDGLKKESERDRAMLALTS TLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPS PQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEM KRENPQLKQIEGLVKELLEREGLTAAGKIEIKI VRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPG AEEAQKERQRQKELESNYRRVWGSPGGEGTGDL DEFDF*DQHYT*PFTESRLFLDWLASSPPPHPG TPEGQTAEWS*AVDLSGNSVGVGALGECCCPCWQ PP*DLTGPVIFALLNSHSILFLSSVAFPVIVP* **DIPCCLPGDSVGSFEWRWVERARRAGHLAGT EQAGPHLPLVMFGVVLPSSIELPWDFLLPFPAHP VPYNLCF*VEEPSPLLLRKW*NAAYHLQHNPSE KGVKHPPCS*TIRFLNKQLDHQ