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Sequences

Sequence NM_018307

GCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCACAGCCCGCTGGGCCGGAGGAGGCGGAGCTGGCGCTGTCCCGGCTCTCTTGC GGGGAAGCAACTGAGGGGGCGGCGCGGCGGGCCCCGGCGGCCGAAGAGGCTGGCAGGTGGCGCCGTGGGGTGGGTGCTCCTGGTGAGAGGAGTCCACTCC GTGCGTGCGGGCGGAGGCCGGCCCCCGAGAGCCGCCGACATGAAGAAAGACGTGCGGATCCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGA TTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCAGAGAGAGTTCCAACACA CATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGTATGCCGTTAACAACAAG CATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATTGGTTGGGAACAAATCTG ATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAAAACCTGAAGAA CATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAA GCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACTCCAT TAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTT ACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTG TTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAAGCATGATTTGGATAGAG ACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAG AGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGGGCTATCTAGGCTATTCA ATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGAT GTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATTCGTGAAGATCATAA ATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATC ATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGCAGAATAC CTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACC ACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCGCACGTGACACAAGCTGACCTC AAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATTGAAACAGCGATGATATA AAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATGTACATTCTGAATGCTTTAAGTTCTGCTAGAATTATTGAGATATTTATACATGCAGAGTTA CTTTATTAATATTTGTAATTCATGCATAAGAGTATTTTAATGATAGTTATAACTGCAGTATTGGCTAGCATATGGAAAGAAAACAGCTAACAGCCAAACT AAAATGGCTAAATTCCAGAGGCCAAAAGGGAATATTTTGTAAATATATGTACATATTCAGGCAAGATATGGTCTCCCAAGCTGAGTTCTAGAAATGATGT TTCTAGACATTTCTAAGTGGTATTGTTAGTGCTCACTTGGCTCACTCTTCTAGGTTTAAGTTAGCCCAGAGATTGTATTTACTCATGGATCACTTTATTT ATTTCACATTTACTCAGAATGATCCTTTGGGTTCTATAAGGACATAAGGTACAATTTGCCATTGTCTCTCCATTTTTAAAAACATACAAGTCAGTGTCAG CTTACCAACATGACATTTTTTCAGTCAGTTGTGGTAGGCCAGCCTTGAAGCCATCGCACAGTCTAGAAACTTGTGTAGCTGAGTGTGCAGCTCACCTTTA AGGGTGAAGTTAGGTAAAAGCAATTAGCAGAGGCGTTATCTATGTGATTATGTTGCTTCCTTGTCAGTATGTTGAATTTTATAGCCCTTTCAATGAAATA AAAAAAAAATTTGTATATTACCAATGTTTTTAGTTTAAATAAAGAGTCACCCTTACTACTGTTGAATTTCATCCCAAGTGTAAATCATTCTATAATGGCT GTGTCTGTTATAGTATATTACAGTAACTGCATGTGTCACCAAGTGTTCTATATCAGGCTAGGATAACCTAGAGGCAGTAATTTTTTAAATGATAAAATAA ATCTAATGAATATAAACTCTCATGATAAACCTATTTTTTCCATCATCAGCCTTTTCAAGTATTTAAATAAATAACTGCTGTGTACTGTGA

refseq_RHOT1.F2 (forward primer)
refseq_RHOT1.R2 (reverse primer)
Single exon skipping

Frame 0

AAAAAAAAAAAAAAAATARWAGGGGAGAVPALL RGSN*GGGAAGPGGRRGWQVAPWGGCSW*EESTP CVRAEAGPREPPT*RKTCGSCWWENLELGRHH* LCLWSVKNFQKRFLPGQKKSPFQLMSPQREFQH TL*ITQKQNRVMNNFIKKYLRLMSSV*CMPLTTS ILLIR*QVDGFLS*MKEQTKTAGCL*YWLGTNL IWWNIVVWRPSFLL*TSIQK*KPVWSVQRKT*R TYQSSFITHRKLFFILQGPCTAQRRRR*NQLV*K PLLVYLKYLIKIMMVLSMMLNSTSFRGFVSTLH *LLKLWRMSRM*SENI*VMVWLTVG*P*KVFSF YTHFLSREGDTKLLGLCFDDLVMMMTWI*HLNIC SPC*KYLLIALLN*IIMHIYFSKAPLTSMIWIE TVLCHLMSLKIYLKFSLTYLGGQM*ITQFVPMK EAG*PTRDSFPSGRSRLI*MYSGAWNIWAI*AIQ Y*LSKSLKLQLLQ*QEIKR*TCRKNKLKEMCSD VM*LE*KTVGKVEFFRLFLEET**GRRKFVKII NPTMRLTLFMYMDKRNTCCCMISQNRNF*LKLKS FVMLYAWYMMSAIPNPLNTVPGFLSNTLWTAEY LA*S*LQSQTCMKLNKNTVFHLLISAGNTKCLH HKPSLAILLMPPVRISLLN*QQWPCIRT*HKLTS RAPRFGFEQVLVLLFLQFWALLCTKHY*NSDDI KRNTVPTKNKYFYVHSECFKFC*NY*DIYTCRV TLLIFVIHA*EYFNDSYNCSIG*HMERKQLTAKL KWLNSRGQKGIFCKYMYIFRQDMVSQAEF*K*C F*TFLSGIVSAHLAHSSRFKLAQRLYLLMDHFI YFTFTQNDPLGSIRT*GTICHCLSIFKNIQVSVS LPT*HFFSQLW*ASLEAIAQSRNLCS*VCSSPL RVKLGKSN*QRRYLCDYVASLSVC*IL*PFQ*N KKKICILPMFLV*IKSHPYYC*ISSQV*IIL*WL CLL*YITVTACVTKCSISG*DNLEAVIF*MIK* I**I*TLMINLFFPSSAFSSI*INNCCVL*

Frame 1

PPPPPPPPPPPPPPPPQPAGPEEAELALSRLSC GEATEGAARRAPAAEEAGRWRRGVGAPGERSPL RACGRRPAPESRRHEERRADPAGGRT*SWEDITD YVSGQ*RISRRGSSPGRRNHHSS*CHPRESSNT HCRLLRSRTE**TTSSRNISG*CHLYSVCR*QQ AFY**GNKSMDSSHK*KNRQRQQAAFNIGWEQI* SGGI**YGDHPSYYEPVYRNRNLCGVFSEKPEE HIRALLLRTESCSSSYRAPVLPRGEGDETSLYK SPYSYI*NI*SR**WYSQ*C*TQLLSEDLFQHSI SSSSSGGCQECSQKTYK*WCG*QWVDPERFSLF THTFYPERETRNYLDCASTIWL***PGFDT*IF VPPAENTS*LHY*IKSSCIFISPKHL*QA*FG*R LCFVT**A*RFI*SFPLHTLGARCE*HSLYQ*K RLDNLPGIPFPVDAHDLFRCTAVPGIFGLSRLF NID*ARVSSFSCYSDKR*KDRPAEKTNSKKCVQM *CNWSEKLWEKWSSSGSSWKKLNEAEENS*RS* ILLCD*HCLCIWTREILVVA*YLRIGISN*S*N HL*CCMPGI*CQQSQIL*ILCQDF*ATLYGQQNT LLNRSCKVRPA*S*TRIQYFTY*FLQETQNAST TSLHLQYC*CPQ*GYLC*IDNNGHVSARDTS*P QELHVLASSKFWCYCFCSFGLCYVQSIIETAMI* KEILSLPKTNTFMYILNALSSARIIEIFIHAEL LY*YL*FMHKSILMIVITAVLASIWKENS*QPN *NG*IPEAKREYFVNICTYSGKIWSPKLSSRNDV SRHF*VVLLVLTWLTLLGLS*PRDCIYSWITLF ISHLLRMILWVL*GHKVQFAIVSPFLKTYKSVS AYQHDIFSVSCGRPALKPSHSLETCVAECAAHL* G*S*VKAISRGVIYVIMLLPCQYVEFYSPFNEI KKKFVYYQCF*FK*RVTLTTVEFHPKCKSFYNG CVCYSILQ*LHVSPSVLYQARIT*RQ*FFK**NK SNEYKLS**TYFFHHQPFQVFK*ITAVYC

Frame 2

RRRRRRRRRRRRRRRHSPLGRRRRSWRCPGSLA GKQLRGRRGGPRRPKRLAGGAVGWVLLVRGVHS VRAGGGRPPRAADMKKDVRILLVGEPRVGKTSL IMSLVSEEFPEEVPPRAEEITIPADVTPERVPTH IVDYSEAEQSDEQLHQEISQANVICIVYAVNNK HSIDKVTSRWIPLINERTDKDSRLPLILVGNKS DLVEYSSMETILPIMNQYTEIETCVECSAKNLKN ISELFYYAQKAVLHPTGPLYCPEEKEMKPACIK ALTRIFKISDQDNDGTLNDAELNFFQRICFNTP LAPQALEDVKNVVRKHISDGVADSGLTLKGFLFL HTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYL FPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDR DCALSPDELKDLFKVFPYIPWGPDVNNTVCTNER GWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYS ILTEQESQASAVTVTRDKKIDLQKKQTQRNVFR CNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHK SYYAINTVYVYGQEKYLLLHDISESEFLTEAEI ICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRI PCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPP QAFTCNTADAPSKDIFVKLTTMAMYPHVTQADL KSSTFWLRASFGATVFAVLGFAMYKALLKQR*Y KKKYCPYQKQILLCTF*ML*VLLELLRYLYMQSY FINICNSCIRVF***L*LQYWLAYGKKTANSQT KMAKFQRPKGNIL*IYVHIQARYGLPS*VLEMM FLDISKWYC*CSLGSLF*V*VSPEIVFTHGSLYL FHIYSE*SFGFYKDIRYNLPLSLHF*KHTSQCQ LTNMTFFQSVVVGQP*SHRTV*KLV*LSVQLTF KGEVR*KQLAEALSM*LCCFLVSMLNFIALSMK* KKNLYITNVFSLNKESPLLLLNFIPSVNHSIMA VSVIVYYSNCMCHQVFYIRLG*PRGSNFLNDKI NLMNINSHDKPIFSIISLFKYLNK*LLCTV

Sequence NM_001033566

GCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCACAGCCCGCTGGGCCGGAGGAGGCGGAGCTGGCGCTGTCCCGGCTCTCTTGC GGGGAAGCAACTGAGGGGGCGGCGCGGCGGGCCCCGGCGGCCGAAGAGGCTGGCAGGTGGCGCCGTGGGGTGGGTGCTCCTGGTGAGAGGAGTCCACTCC GTGCGTGCGGGCGGAGGCCGGCCCCCGAGAGCCGCCGACATGAAGAAAGACGTGCGGATCCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGA TTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCAGAGAGAGTTCCAACACA CATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGTATGCCGTTAACAACAAG CATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATTGGTTGGGAACAAATCTG ATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAAAACCTGAAGAA CATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAA GCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACTCCAT TAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTT ACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTG TTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAAGCATGATTTGGATAGAG ACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAG AGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGGGCTATCTAGGCTATTCA ATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGAT GTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATTCGTGAAGATCATAA ATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATC ATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGCAGAATAC CTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACC ACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCCCATGCCCGGTTACGCTGTATG TGCACCTGCAACAGGTGTACATTTTGCATCTGTCAGAACTTCCTCAACTCAGACTTGCTGCAATCTGTAAAGAACAAAATCTTCACTGCAGTTCTTAACA GGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGC ATTATTGAAACAGCGATGATATAAAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATGTACATTCTGAATGCTTTAAGTTCTGCTAGAATTATTG AGATATTTATACATGCAGAGTTACTTTATTAATATTTGTAATTCATGCATAAGAGTATTTTAATGATAGTTATAACTGCAGTATTGGCTAGCATATGGAA AGAAAACAGCTAACAGCCAAACTAAAATGGCTAAATTCCAGAGGCCAAAAGGGAATATTTTGTAAATATATGTACATATTCAGGCAAGATATGGTCTCCC AAGCTGAGTTCTAGAAATGATGTTTCTAGACATTTCTAAGTGGTATTGTTAGTGCTCACTTGGCTCACTCTTCTAGGTTTAAGTTAGCCCAGAGATTGTA TTTACTCATGGATCACTTTATTTATTTCACATTTACTCAGAATGATCCTTTGGGTTCTATAAGGACATAAGGTACAATTTGCCATTGTCTCTCCATTTTT AAAAACATACAAGTCAGTGTCAGCTTACCAACATGACATTTTTTCAGTCAGTTGTGGTAGGCCAGCCTTGAAGCCATCGCACAGTCTAGAAACTTGTGTA GCTGAGTGTGCAGCTCACCTTTAAGGGTGAAGTTAGGTAAAAGCAATTAGCAGAGGCGTTATCTATGTGATTATGTTGCTTCCTTGTCAGTATGTTGAAT TTTATAGCCCTTTCAATGAAATAAAAAAAAAATTTGTATATTACCAATGTTTTTAGTTTAAATAAAGAGTCACCCTTACTACTGTTGAATTTCATCCCAA GTGTAAATCATTCTATAATGGCTGTGTCTGTTATAGTATATTACAGTAACTGCATGTGTCACCAAGTGTTCTATATCAGGCTAGGATAACCTAGAGGCAG TAATTTTTTAAATGATAAAATAAATCTAATGAATATAAACTCTCATGATAAACCTATTTTTTCCATCATCAGCCTTTTCAAGTATTTAAATAAATAACTG CTGTGTACTGTGA

refseq_RHOT1.F2 (forward primer)
refseq_RHOT1.R2 (reverse primer)
Single exon skipping

Frame 0

AAAAAAAAAAAAAAAATARWAGGGGAGAVPALL RGSN*GGGAAGPGGRRGWQVAPWGGCSW*EESTP CVRAEAGPREPPT*RKTCGSCWWENLELGRHH* LCLWSVKNFQKRFLPGQKKSPFQLMSPQREFQH TL*ITQKQNRVMNNFIKKYLRLMSSV*CMPLTTS ILLIR*QVDGFLS*MKEQTKTAGCL*YWLGTNL IWWNIVVWRPSFLL*TSIQK*KPVWSVQRKT*R TYQSSFITHRKLFFILQGPCTAQRRRR*NQLV*K PLLVYLKYLIKIMMVLSMMLNSTSFRGFVSTLH *LLKLWRMSRM*SENI*VMVWLTVG*P*KVFSF YTHFLSREGDTKLLGLCFDDLVMMMTWI*HLNIC SPC*KYLLIALLN*IIMHIYFSKAPLTSMIWIE TVLCHLMSLKIYLKFSLTYLGGQM*ITQFVPMK EAG*PTRDSFPSGRSRLI*MYSGAWNIWAI*AIQ Y*LSKSLKLQLLQ*QEIKR*TCRKNKLKEMCSD VM*LE*KTVGKVEFFRLFLEET**GRRKFVKII NPTMRLTLFMYMDKRNTCCCMISQNRNF*LKLKS FVMLYAWYMMSAIPNPLNTVPGFLSNTLWTAEY LA*S*LQSQTCMKLNKNTVFHLLISAGNTKCLH HKPSLAILLMPPVRISLLN*QQWPCIPMPGYAVC APATGVHFASVRTSSTQTCCNL*RTKSSLQFLT GT*HKLTSRAPRFGFEQVLVLLFLQFWALLCTK HY*NSDDIKRNTVPTKNKYFYVHSECFKFC*NY* DIYTCRVTLLIFVIHA*EYFNDSYNCSIG*HME RKQLTAKLKWLNSRGQKGIFCKYMYIFRQDMVS QAEF*K*CF*TFLSGIVSAHLAHSSRFKLAQRLY LLMDHFIYFTFTQNDPLGSIRT*GTICHCLSIF KNIQVSVSLPT*HFFSQLW*ASLEAIAQSRNLC S*VCSSPLRVKLGKSN*QRRYLCDYVASLSVC*I L*PFQ*NKKKICILPMFLV*IKSHPYYC*ISSQ V*IIL*WLCLL*YITVTACVTKCSISG*DNLEA VIF*MIK*I**I*TLMINLFFPSSAFSSI*INNC CVL*

Frame 1

PPPPPPPPPPPPPPPPQPAGPEEAELALSRLSC GEATEGAARRAPAAEEAGRWRRGVGAPGERSPL RACGRRPAPESRRHEERRADPAGGRT*SWEDITD YVSGQ*RISRRGSSPGRRNHHSS*CHPRESSNT HCRLLRSRTE**TTSSRNISG*CHLYSVCR*QQ AFY**GNKSMDSSHK*KNRQRQQAAFNIGWEQI* SGGI**YGDHPSYYEPVYRNRNLCGVFSEKPEE HIRALLLRTESCSSSYRAPVLPRGEGDETSLYK SPYSYI*NI*SR**WYSQ*C*TQLLSEDLFQHSI SSSSSGGCQECSQKTYK*WCG*QWVDPERFSLF THTFYPERETRNYLDCASTIWL***PGFDT*IF VPPAENTS*LHY*IKSSCIFISPKHL*QA*FG*R LCFVT**A*RFI*SFPLHTLGARCE*HSLYQ*K RLDNLPGIPFPVDAHDLFRCTAVPGIFGLSRLF NID*ARVSSFSCYSDKR*KDRPAEKTNSKKCVQM *CNWSEKLWEKWSSSGSSWKKLNEAEENS*RS* ILLCD*HCLCIWTREILVVA*YLRIGISN*S*N HL*CCMPGI*CQQSQIL*ILCQDF*ATLYGQQNT LLNRSCKVRPA*S*TRIQYFTY*FLQETQNAST TSLHLQYC*CPQ*GYLC*IDNNGHVSPCPVTLY VHLQQVYILHLSELPQLRLAAICKEQNLHCSS*Q ARDTS*PQELHVLASSKFWCYCFCSFGLCYVQS IIETAMI*KEILSLPKTNTFMYILNALSSARII EIFIHAELLY*YL*FMHKSILMIVITAVLASIWK ENS*QPN*NG*IPEAKREYFVNICTYSGKIWSP KLSSRNDVSRHF*VVLLVLTWLTLLGLS*PRDC IYSWITLFISHLLRMILWVL*GHKVQFAIVSPFL KTYKSVSAYQHDIFSVSCGRPALKPSHSLETCV AECAAHL*G*S*VKAISRGVIYVIMLLPCQYVE FYSPFNEIKKKFVYYQCF*FK*RVTLTTVEFHPK CKSFYNGCVCYSILQ*LHVSPSVLYQARIT*RQ *FFK**NKSNEYKLS**TYFFHHQPFQVFK*IT AVYC

Frame 2

RRRRRRRRRRRRRRRHSPLGRRRRSWRCPGSLA GKQLRGRRGGPRRPKRLAGGAVGWVLLVRGVHS VRAGGGRPPRAADMKKDVRILLVGEPRVGKTSL IMSLVSEEFPEEVPPRAEEITIPADVTPERVPTH IVDYSEAEQSDEQLHQEISQANVICIVYAVNNK HSIDKVTSRWIPLINERTDKDSRLPLILVGNKS DLVEYSSMETILPIMNQYTEIETCVECSAKNLKN ISELFYYAQKAVLHPTGPLYCPEEKEMKPACIK ALTRIFKISDQDNDGTLNDAELNFFQRICFNTP LAPQALEDVKNVVRKHISDGVADSGLTLKGFLFL HTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYL FPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDR DCALSPDELKDLFKVFPYIPWGPDVNNTVCTNER GWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYS ILTEQESQASAVTVTRDKKIDLQKKQTQRNVFR CNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHK SYYAINTVYVYGQEKYLLLHDISESEFLTEAEI ICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRI PCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPP QAFTCNTADAPSKDIFVKLTTMAMYPHARLRCM CTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLN RHVTQADLKSSTFWLRASFGATVFAVLGFAMYKA LLKQR*YKKKYCPYQKQILLCTF*ML*VLLELL RYLYMQSYFINICNSCIRVF***L*LQYWLAYG KKTANSQTKMAKFQRPKGNIL*IYVHIQARYGLP S*VLEMMFLDISKWYC*CSLGSLF*V*VSPEIV FTHGSLYLFHIYSE*SFGFYKDIRYNLPLSLHF *KHTSQCQLTNMTFFQSVVVGQP*SHRTV*KLV* LSVQLTFKGEVR*KQLAEALSM*LCCFLVSMLN FIALSMK*KKNLYITNVFSLNKESPLLLLNFIP SVNHSIMAVSVIVYYSNCMCHQVFYIRLG*PRGS NFLNDKINLMNINSHDKPIFSIISLFKYLNK*L LCTV

Sequence NM_001033568

GCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCACAGCCCGCTGGGCCGGAGGAGGCGGAGCTGGCGCTGTCCCGGCTCTCTTGC GGGGAAGCAACTGAGGGGGCGGCGCGGCGGGCCCCGGCGGCCGAAGAGGCTGGCAGGTGGCGCCGTGGGGTGGGTGCTCCTGGTGAGAGGAGTCCACTCC GTGCGTGCGGGCGGAGGCCGGCCCCCGAGAGCCGCCGACATGAAGAAAGACGTGCGGATCCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGA TTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCAGAGAGAGTTCCAACACA CATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGTATGCCGTTAACAACAAG CATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATTGGTTGGGAACAAATCTG ATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAAAACCTGAAGAA CATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAA GCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACTCCAT TAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTT ACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTG TTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAAGCATGATTTGGATAGAG ACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAG AGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGGGCTATCTAGGCTATTCA ATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGAT GTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATTCGTGAAGATCATAA ATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATC ATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGCAGAATAC CTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACC ACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCAGAGGATCATTACAGAGACAGA CTCTCCCGAGACATGGGCCACACTGATAGAATAGAGAATTTGAGAAAAATCTGGGTCTTTCTAAAAACTGCTTTCCATGCCCGGTTACGCTGTATGTGCA CCTGCAACAGGTGTACATTTTGCATCTGTCAGAACTTCCTCAACTCAGACTTGCTGCAATCTGTAAAGAACAAAATCTTCACTGCAGTTCTTAACAGGCA CGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTA TTGAAACAGCGATGATATAAAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATGTACATTCTGAATGCTTTAAGTTCTGCTAGAATTATTGAGAT ATTTATACATGCAGAGTTACTTTATTAATATTTGTAATTCATGCATAAGAGTATTTTAATGATAGTTATAACTGCAGTATTGGCTAGCATATGGAAAGAA AACAGCTAACAGCCAAACTAAAATGGCTAAATTCCAGAGGCCAAAAGGGAATATTTTGTAAATATATGTACATATTCAGGCAAGATATGGTCTCCCAAGC TGAGTTCTAGAAATGATGTTTCTAGACATTTCTAAGTGGTATTGTTAGTGCTCACTTGGCTCACTCTTCTAGGTTTAAGTTAGCCCAGAGATTGTATTTA CTCATGGATCACTTTATTTATTTCACATTTACTCAGAATGATCCTTTGGGTTCTATAAGGACATAAGGTACAATTTGCCATTGTCTCTCCATTTTTAAAA ACATACAAGTCAGTGTCAGCTTACCAACATGACATTTTTTCAGTCAGTTGTGGTAGGCCAGCCTTGAAGCCATCGCACAGTCTAGAAACTTGTGTAGCTG AGTGTGCAGCTCACCTTTAAGGGTGAAGTTAGGTAAAAGCAATTAGCAGAGGCGTTATCTATGTGATTATGTTGCTTCCTTGTCAGTATGTTGAATTTTA TAGCCCTTTCAATGAAATAAAAAAAAAATTTGTATATTACCAATGTTTTTAGTTTAAATAAAGAGTCACCCTTACTACTGTTGAATTTCATCCCAAGTGT AAATCATTCTATAATGGCTGTGTCTGTTATAGTATATTACAGTAACTGCATGTGTCACCAAGTGTTCTATATCAGGCTAGGATAACCTAGAGGCAGTAAT TTTTTAAATGATAAAATAAATCTAATGAATATAAACTCTCATGATAAACCTATTTTTTCCATCATCAGCCTTTTCAAGTATTTAAATAAATAACTGCTGT GTACTGTGA

refseq_RHOT1.F2 (forward primer)
refseq_RHOT1.R2 (reverse primer)
Single exon skipping

Frame 0

AAAAAAAAAAAAAAAATARWAGGGGAGAVPALL RGSN*GGGAAGPGGRRGWQVAPWGGCSW*EESTP CVRAEAGPREPPT*RKTCGSCWWENLELGRHH* LCLWSVKNFQKRFLPGQKKSPFQLMSPQREFQH TL*ITQKQNRVMNNFIKKYLRLMSSV*CMPLTTS ILLIR*QVDGFLS*MKEQTKTAGCL*YWLGTNL IWWNIVVWRPSFLL*TSIQK*KPVWSVQRKT*R TYQSSFITHRKLFFILQGPCTAQRRRR*NQLV*K PLLVYLKYLIKIMMVLSMMLNSTSFRGFVSTLH *LLKLWRMSRM*SENI*VMVWLTVG*P*KVFSF YTHFLSREGDTKLLGLCFDDLVMMMTWI*HLNIC SPC*KYLLIALLN*IIMHIYFSKAPLTSMIWIE TVLCHLMSLKIYLKFSLTYLGGQM*ITQFVPMK EAG*PTRDSFPSGRSRLI*MYSGAWNIWAI*AIQ Y*LSKSLKLQLLQ*QEIKR*TCRKNKLKEMCSD VM*LE*KTVGKVEFFRLFLEET**GRRKFVKII NPTMRLTLFMYMDKRNTCCCMISQNRNF*LKLKS FVMLYAWYMMSAIPNPLNTVPGFLSNTLWTAEY LA*S*LQSQTCMKLNKNTVFHLLISAGNTKCLH HKPSLAILLMPPVRISLLN*QQWPCIQRIITETD SPETWATLIE*RI*EKSGSF*KLLSMPGYAVCA PATGVHFASVRTSSTQTCCNL*RTKSSLQFLTG T*HKLTSRAPRFGFEQVLVLLFLQFWALLCTKHY *NSDDIKRNTVPTKNKYFYVHSECFKFC*NY*D IYTCRVTLLIFVIHA*EYFNDSYNCSIG*HMER KQLTAKLKWLNSRGQKGIFCKYMYIFRQDMVSQA EF*K*CF*TFLSGIVSAHLAHSSRFKLAQRLYL LMDHFIYFTFTQNDPLGSIRT*GTICHCLSIFK NIQVSVSLPT*HFFSQLW*ASLEAIAQSRNLCS* VCSSPLRVKLGKSN*QRRYLCDYVASLSVC*IL *PFQ*NKKKICILPMFLV*IKSHPYYC*ISSQV *IIL*WLCLL*YITVTACVTKCSISG*DNLEAVI F*MIK*I**I*TLMINLFFPSSAFSSI*INNCC VL*

Frame 1

PPPPPPPPPPPPPPPPQPAGPEEAELALSRLSC GEATEGAARRAPAAEEAGRWRRGVGAPGERSPL RACGRRPAPESRRHEERRADPAGGRT*SWEDITD YVSGQ*RISRRGSSPGRRNHHSS*CHPRESSNT HCRLLRSRTE**TTSSRNISG*CHLYSVCR*QQ AFY**GNKSMDSSHK*KNRQRQQAAFNIGWEQI* SGGI**YGDHPSYYEPVYRNRNLCGVFSEKPEE HIRALLLRTESCSSSYRAPVLPRGEGDETSLYK SPYSYI*NI*SR**WYSQ*C*TQLLSEDLFQHSI SSSSSGGCQECSQKTYK*WCG*QWVDPERFSLF THTFYPERETRNYLDCASTIWL***PGFDT*IF VPPAENTS*LHY*IKSSCIFISPKHL*QA*FG*R LCFVT**A*RFI*SFPLHTLGARCE*HSLYQ*K RLDNLPGIPFPVDAHDLFRCTAVPGIFGLSRLF NID*ARVSSFSCYSDKR*KDRPAEKTNSKKCVQM *CNWSEKLWEKWSSSGSSWKKLNEAEENS*RS* ILLCD*HCLCIWTREILVVA*YLRIGISN*S*N HL*CCMPGI*CQQSQIL*ILCQDF*ATLYGQQNT LLNRSCKVRPA*S*TRIQYFTY*FLQETQNAST TSLHLQYC*CPQ*GYLC*IDNNGHVSRGSLQRQ TLPRHGPH**NREFEKNLGLSKNCFPCPVTLYVH LQQVYILHLSELPQLRLAAICKEQNLHCSS*QA RDTS*PQELHVLASSKFWCYCFCSFGLCYVQSI IETAMI*KEILSLPKTNTFMYILNALSSARIIEI FIHAELLY*YL*FMHKSILMIVITAVLASIWKE NS*QPN*NG*IPEAKREYFVNICTYSGKIWSPK LSSRNDVSRHF*VVLLVLTWLTLLGLS*PRDCIY SWITLFISHLLRMILWVL*GHKVQFAIVSPFLK TYKSVSAYQHDIFSVSCGRPALKPSHSLETCVA ECAAHL*G*S*VKAISRGVIYVIMLLPCQYVEFY SPFNEIKKKFVYYQCF*FK*RVTLTTVEFHPKC KSFYNGCVCYSILQ*LHVSPSVLYQARIT*RQ* FFK**NKSNEYKLS**TYFFHHQPFQVFK*ITAV YC

Frame 2

RRRRRRRRRRRRRRRHSPLGRRRRSWRCPGSLA GKQLRGRRGGPRRPKRLAGGAVGWVLLVRGVHS VRAGGGRPPRAADMKKDVRILLVGEPRVGKTSL IMSLVSEEFPEEVPPRAEEITIPADVTPERVPTH IVDYSEAEQSDEQLHQEISQANVICIVYAVNNK HSIDKVTSRWIPLINERTDKDSRLPLILVGNKS DLVEYSSMETILPIMNQYTEIETCVECSAKNLKN ISELFYYAQKAVLHPTGPLYCPEEKEMKPACIK ALTRIFKISDQDNDGTLNDAELNFFQRICFNTP LAPQALEDVKNVVRKHISDGVADSGLTLKGFLFL HTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYL FPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDR DCALSPDELKDLFKVFPYIPWGPDVNNTVCTNER GWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYS ILTEQESQASAVTVTRDKKIDLQKKQTQRNVFR CNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHK SYYAINTVYVYGQEKYLLLHDISESEFLTEAEI ICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRI PCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPP QAFTCNTADAPSKDIFVKLTTMAMYPEDHYRDR LSRDMGHTDRIENLRKIWVFLKTAFHARLRCMC TCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRH VTQADLKSSTFWLRASFGATVFAVLGFAMYKAL LKQR*YKKKYCPYQKQILLCTF*ML*VLLELLR YLYMQSYFINICNSCIRVF***L*LQYWLAYGKK TANSQTKMAKFQRPKGNIL*IYVHIQARYGLPS *VLEMMFLDISKWYC*CSLGSLF*V*VSPEIVF THGSLYLFHIYSE*SFGFYKDIRYNLPLSLHF*K HTSQCQLTNMTFFQSVVVGQP*SHRTV*KLV*L SVQLTFKGEVR*KQLAEALSM*LCCFLVSMLNF IALSMK*KKNLYITNVFSLNKESPLLLLNFIPSV NHSIMAVSVIVYYSNCMCHQVFYIRLG*PRGSN FLNDKINLMNINSHDKPIFSIISLFKYLNK*LL CTV

Sequence NM_001033567

GCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCACAGCCCGCTGGGCCGGAGGAGGCGGAGCTGGCGCTGTCCCGGCTCTCTTGC GGGGAAGCAACTGAGGGGGCGGCGCGGCGGGCCCCGGCGGCCGAAGAGGCTGGCAGGTGGCGCCGTGGGGTGGGTGCTCCTGGTGAGAGGAGTCCACTCC GTGCGTGCGGGCGGAGGCCGGCCCCCGAGAGCCGCCGACATGAAGAAAGACGTGCGGATCCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGA TTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGATTGTTGTGGATTTAAATACCTCCTCATTTGCAGAGTATCTTCAGCCTTCAACAAAATAGAATGA AAACAACATATTTAAAAAATCTAACACCGAAACACCAAATTAATCCAAGAATCCACTGAGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGT CACCCCAGAGAGAGTTCCAACACACATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGT ATAGTGTATGCCGTTAACAACAAGCATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTT TAATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGA GTGTTCAGCGAAAAACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAG GAGATGAAACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTC AGAGGATTTGTTTCAACACTCCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTT GACCCTGAAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGAC CTGGATTTGACACCTGAATATTTGTTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCT TTGACAAGCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAA TAACACAGTTTGTACCAATGAAAGAGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAA TATTTGGGCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAAC AAACTCAAAGAAATGTGTTCAGATGTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCA GAAGAAAATTCGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCG GAATTTCTAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGC AACACTTTATGGACAGCAGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTG CAGGAAACACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTAT CCGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAG CATTATTGAAACAGCGATGATATAAAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATGTACATTCTGAATGCTTTAAGTTCTGCTAGAATTATT GAGATATTTATACATGCAGAGTTACTTTATTAATATTTGTAATTCATGCATAAGAGTATTTTAATGATAGTTATAACTGCAGTATTGGCTAGCATATGGA AAGAAAACAGCTAACAGCCAAACTAAAATGGCTAAATTCCAGAGGCCAAAAGGGAATATTTTGTAAATATATGTACATATTCAGGCAAGATATGGTCTCC CAAGCTGAGTTCTAGAAATGATGTTTCTAGACATTTCTAAGTGGTATTGTTAGTGCTCACTTGGCTCACTCTTCTAGGTTTAAGTTAGCCCAGAGATTGT ATTTACTCATGGATCACTTTATTTATTTCACATTTACTCAGAATGATCCTTTGGGTTCTATAAGGACATAAGGTACAATTTGCCATTGTCTCTCCATTTT TAAAAACATACAAGTCAGTGTCAGCTTACCAACATGACATTTTTTCAGTCAGTTGTGGTAGGCCAGCCTTGAAGCCATCGCACAGTCTAGAAACTTGTGT AGCTGAGTGTGCAGCTCACCTTTAAGGGTGAAGTTAGGTAAAAGCAATTAGCAGAGGCGTTATCTATGTGATTATGTTGCTTCCTTGTCAGTATGTTGAA TTTTATAGCCCTTTCAATGAAATAAAAAAAAAATTTGTATATTACCAATGTTTTTAGTTTAAATAAAGAGTCACCCTTACTACTGTTGAATTTCATCCCA AGTGTAAATCATTCTATAATGGCTGTGTCTGTTATAGTATATTACAGTAACTGCATGTGTCACCAAGTGTTCTATATCAGGCTAGGATAACCTAGAGGCA GTAATTTTTTAAATGATAAAATAAATCTAATGAATATAAACTCTCATGATAAACCTATTTTTTCCATCATCAGCCTTTTCAAGTATTTAAATAAATAACT GCTGTGTACTGTGA

refseq_RHOT1.F2 (forward primer)
refseq_RHOT1.R2 (reverse primer)
Single exon skipping

Frame 0

AAAAAAAAAAAAAAAATARWAGGGGAGAVPALL RGSN*GGGAAGPGGRRGWQVAPWGGCSW*EESTP CVRAEAGPREPPT*RKTCGSCWWENLELGRHH* LCLWSVKNFQKRLLWI*IPPHLQSIFSLQQNRM KTTYLKNLTPKHQINPRIH*VPPRAEEITIPADV TPERVPTHIVDYSEAEQSDEQLHQEISQANVIC IVYAVNNKHSIDKVTSRWIPLINERTDKDSRLP LILVGNKSDLVEYSSMETILPIMNQYTEIETCVE CSAKNLKNISELFYYAQKAVLHPTGPLYCPEEK EMKPACIKALTRIFKISDQDNDGTLNDAELNFF QRICFNTPLAPQALEDVKNVVRKHISDGVADSGL TLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDD LDLTPEYLFPLLKIPPDCTTELNHHAYLFLQST FDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVN NTVCTNERGWITYQGFLSQWTLTTYLDVQRCLE YLGYLGYSILTEQESQASAVTVTRDKKIDLQKK QTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQ KKIREDHKSYYAINTVYVYGQEKYLLLHDISES EFLTEAEIICDVVCLVYDVSNPKSFEYCARIFK QHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFC RKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMY PHVTQADLKSSTFWLRASFGATVFAVLGFAMYK ALLKQR*YKKKYCPYQKQILLCTF*ML*VLLELL RYLYMQSYFINICNSCIRVF***L*LQYWLAYG KKTANSQTKMAKFQRPKGNIL*IYVHIQARYGL PS*VLEMMFLDISKWYC*CSLGSLF*V*VSPEIV FTHGSLYLFHIYSE*SFGFYKDIRYNLPLSLHF *KHTSQCQLTNMTFFQSVVVGQP*SHRTV*KLV *LSVQLTFKGEVR*KQLAEALSM*LCCFLVSMLN FIALSMK*KKNLYITNVFSLNKESPLLLLNFIP SVNHSIMAVSVIVYYSNCMCHQVFYIRLG*PRG SNFLNDKINLMNINSHDKPIFSIISLFKYLNK*L LCTV

Frame 1

PPPPPPPPPPPPPPPPQPAGPEEAELALSRLSC GEATEGAARRAPAAEEAGRWRRGVGAPGERSPL RACGRRPAPESRRHEERRADPAGGRT*SWEDITD YVSGQ*RISRRDCCGFKYLLICRVSSAFNKIE* KQHI*KI*HRNTKLIQESTEFLPGQKKSPFQLM SPQREFQHTL*ITQKQNRVMNNFIKKYLRLMSSV *CMPLTTSILLIR*QVDGFLS*MKEQTKTAGCL *YWLGTNLIWWNIVVWRPSFLL*TSIQK*KPVW SVQRKT*RTYQSSFITHRKLFFILQGPCTAQRRR R*NQLV*KPLLVYLKYLIKIMMVLSMMLNSTSF RGFVSTLH*LLKLWRMSRM*SENI*VMVWLTVG *P*KVFSFYTHFLSREGDTKLLGLCFDDLVMMMT WI*HLNICSPC*KYLLIALLN*IIMHIYFSKAP LTSMIWIETVLCHLMSLKIYLKFSLTYLGGQM* ITQFVPMKEAG*PTRDSFPSGRSRLI*MYSGAWN IWAI*AIQY*LSKSLKLQLLQ*QEIKR*TCRKN KLKEMCSDVM*LE*KTVGKVEFFRLFLEET**G RRKFVKIINPTMRLTLFMYMDKRNTCCCMISQNR NF*LKLKSFVMLYAWYMMSAIPNPLNTVPGFLS NTLWTAEYLA*S*LQSQTCMKLNKNTVFHLLIS AGNTKCLHHKPSLAILLMPPVRISLLN*QQWPCI RT*HKLTSRAPRFGFEQVLVLLFLQFWALLCTK HY*NSDDIKRNTVPTKNKYFYVHSECFKFC*NY *DIYTCRVTLLIFVIHA*EYFNDSYNCSIG*HME RKQLTAKLKWLNSRGQKGIFCKYMYIFRQDMVS QAEF*K*CF*TFLSGIVSAHLAHSSRFKLAQRL YLLMDHFIYFTFTQNDPLGSIRT*GTICHCLSIF KNIQVSVSLPT*HFFSQLW*ASLEAIAQSRNLC S*VCSSPLRVKLGKSN*QRRYLCDYVASLSVC* IL*PFQ*NKKKICILPMFLV*IKSHPYYC*ISSQ V*IIL*WLCLL*YITVTACVTKCSISG*DNLEA VIF*MIK*I**I*TLMINLFFPSSAFSSI*INN CCVL*

Frame 2

RRRRRRRRRRRRRRRHSPLGRRRRSWRCPGSLA GKQLRGRRGGPRRPKRLAGGAVGWVLLVRGVHS VRAGGGRPPRAADMKKDVRILLVGEPRVGKTSL IMSLVSEEFPEEIVVDLNTSSFAEYLQPSTK*NE NNIFKKSNTETPN*SKNPLSSSPGRRNHHSS*C HPRESSNTHCRLLRSRTE**TTSSRNISG*CHL YSVCR*QQAFY**GNKSMDSSHK*KNRQRQQAAF NIGWEQI*SGGI**YGDHPSYYEPVYRNRNLCG VFSEKPEEHIRALLLRTESCSSSYRAPVLPRGE GDETSLYKSPYSYI*NI*SR**WYSQ*C*TQLLS EDLFQHSISSSSSGGCQECSQKTYK*WCG*QWV DPERFSLFTHTFYPERETRNYLDCASTIWL*** PGFDT*IFVPPAENTS*LHY*IKSSCIFISPKHL *QA*FG*RLCFVT**A*RFI*SFPLHTLGARCE *HSLYQ*KRLDNLPGIPFPVDAHDLFRCTAVPG IFGLSRLFNID*ARVSSFSCYSDKR*KDRPAEKT NSKKCVQM*CNWSEKLWEKWSSSGSSWKKLNEA EENS*RS*ILLCD*HCLCIWTREILVVA*YLRI GISN*S*NHL*CCMPGI*CQQSQIL*ILCQDF*A TLYGQQNTLLNRSCKVRPA*S*TRIQYFTY*FL QETQNASTTSLHLQYC*CPQ*GYLC*IDNNGHV SARDTS*PQELHVLASSKFWCYCFCSFGLCYVQS IIETAMI*KEILSLPKTNTFMYILNALSSARII EIFIHAELLY*YL*FMHKSILMIVITAVLASIW KENS*QPN*NG*IPEAKREYFVNICTYSGKIWSP KLSSRNDVSRHF*VVLLVLTWLTLLGLS*PRDC IYSWITLFISHLLRMILWVL*GHKVQFAIVSPF LKTYKSVSAYQHDIFSVSCGRPALKPSHSLETCV AECAAHL*G*S*VKAISRGVIYVIMLLPCQYVE FYSPFNEIKKKFVYYQCF*FK*RVTLTTVEFHP KCKSFYNGCVCYSILQ*LHVSPSVLYQARIT*RQ *FFK**NKSNEYKLS**TYFFHHQPFQVFK*IT AVYC