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Sequences

Sequence NM_022716

TGATTCGAGCGGGAAGAGGGGGGTGGGTGGGATCGGTGGGGGAGACCATGACCTCCAGCTACGGGCACGTTCTGGAGCGGCAACCGGCGCTGGGCGGCCG CTTGGACAGCCCGGGCAACCTCGACACCCTGCAGGCGAAAAAGAACTTCTCCGTCAGTCACCTGCTAGACCTGGAGGAAGCCGGGGACATGGTGGCGGCA CAGGCGGATGAGAACGTGGGCGAGGCTGGCCGGAGCCTGCTGGAGTCGCCGGGACTCACCAGCGGCAGCGACACCCCGCAGCAGGACAATGACCAGCTGA ACTCAGAAGAAAAAAAGAAGAGAAAGCAGCGAAGGAATAGGACAACCTTCAATAGCAGCCAGCTGCAGGCTTTGGAGCGTGTCTTTGAGCGGACACACTA TCCTGATGCTTTTGTGCGAGAAGACCTTGCCCGCCGGGTGAACCTCACCGAGGCGAGAGTGCAGGTGTGGTTTCAGAACCGAAGAGCCAAGTTCCGCAGG AATGAGAGAGCCATGCTAGCCAATAAAAACGCTTCCCTCCTCAAATCCTACTCAGGAGACGTGACTGCTGTGGAGCAGCCCATCGTACCTCGTCCTGCTC CGAGACCCACCGATTATCTCTCCTGGGGGACAGCGTCTCCGTACAGCGCCATGGCTACTTATTCTGCCACATGTGCCAACAATAGCCCTGCACAGGGCAT CAACATGGCCAACAGCATTGCCAACCTGAGACTGAAGGCCAAGGAATATAGTTTACAGAGGAACCAGGTGCCAACAGTCAACTGAGGAAAAAAAATAATT AAACAGGCCTAAGAAGAAATCAAAAACCATAAGACACCTATCCTGCTCTGTTATTTCTTCATCTGCTGGGGGGAAAAAGTAAATTACAAACAAACAAACA AAGCAGAACTAAAATATTGGGACCATGGCAGAGAAAAGCAGGAGAGGAGCAAAATGAAAATTAGTTAACAAATGTTCCTCCTCCCTCTGGGATACCACCA CCACTTGTTTCTGTGTGTGTTTATTTTGTTTTTCTTTCATTCATGCTTTGCTTAATGTACTCCAGGCTTCTTCAGATAGGTTCAGCCCACCCACCCCCAT GATTGTATGAAGTTTTAAAAAAAACTACAGCAGCCAAAGAAACTATATATATATATATATATATATATATCCAGAATGATTGCCTCTACTGTCCTCATTG ACTTGTTTGAACCTTAGTGCCTTACCCTGTCCTCTTCCCAGTTCTCTTTATAGAAGCTCTAGGAGCTTTCGAAAAGCCAAAGTCTTTCTGAAGAATCTGT GCTGGACAGACATAATTCCCTTTCTCATTGTCTCCATCTTTGTTGGTCATGGTAAGGTTTTTCCATCAGCCTCTGAAAAAATAGTTGTGCACAACATCTG CTCACTGGACTGTCTGATCCAATGTAATTGGCTGCGTCTGGCTAATTCTAAGCACTAAAGTCTACATCTAAGCTATAGATTTAAGCTTGAAGCTACAGAT TATATCACTATCACCACCACCCCTCACCCTATGCAATCAATCAATCAATCATCTTAAGTTAAAGATATTTGTTGTCTTTGAATGATTTGCTGTCACAGAC TATTTGGTAGAAGAAATATTTTTCACCTGAGAGAGGAAGAGAAATTTCTCTAGTAACACAAAGAGTGAGTTCTAAAAGGCATGCCCACATCTCTTTCGTG CCTTAAGGATAGTGAGATGCACACTTATATATATACTGTATATATTTATATATTTATATATATATTTCATATATATATATAATATTGCAAGCTTAAGTTT GCAATTTCCCAAACAATACAAAAAGCAAATTACACACCCTCACCACTGTTCTTATCTCTATAGTGATGAAACATTAATTAGGGATCTTGCTGCTTTTCTT TTTCTACACGAAGTTTTCATTAAAGCCACAGAATAATTGATAGGGCAGCTGTTTGAGAACAGGTCCCATTTTCACATTAGGGCTTTAAATGAATTAGAAA CTATTTGAGGCTATAAAAATGTCCTTGAGTTTGGAGCCTGAGCTCTGGTGAAATGCTGATACATCTGATCTATCATGGGAATTGCAGTTAGAGAGAGTAA GGAATACCATTTAGTCATCTATCCGTTCTTCACTTAGCAGGAATATGAAAGAAAGGCACATGTTTAAGAGGAATACCTAAAGGTTTTTCTAAATTCCAAC ATTTAAAAGGCAATTGTGGGCTATTTTTATTTTTTAATATTTTGAAATAAAGTTTAGTGTCTAGGGCTGGGAGCCAGGACTGATCTTCCATTTCTTTTTC TTTGTTCCCAGCCATGCTTTTGTAACTTGCCAGGTGGACTTGACCAACTACATTACCATGCTGTGCCTCAGTTTACCCATTTGTAAAATGGGATTAATAA TACTTACCTACCTCACAGGGGTGTTGTGAGGCTCTATTCATTTGCTCCTTTATTCTTTCCTGTATTCTCTGTATGTCCAGCACTTTGTAGCCATGGGAGG AAAGGGACTATAAAAGTGTACAATGTTAATGGAATGATACGGTACCTGAAAGCCTTGTTTTCTAGTAAGAAAATGCTACCTTGCTGTACATACTTATAAC CTTGTATTTGGAAATGAGAAATAGGTTTATATTTTCAGATCTCTCAAAAATCACATCATTTGACCAAAGAATAATTTAAGACACATAGAACAGATTTTTT TAATTTATATTTTCATCCTGACCAGCTTAGTTCTAATAATTTTTAGTTGTGAGTGATTAAAAAACTTTGGATCAATTTTGGTCAAACATGCCAACTTTGT AGTCTGAGTGACAGGCAAGGATTTTTGGGTTTAAGATGCACTTTTAGCACACATTTGTATTTCCCTTGGCATATCAGATTGAGCTAATGGTGATGTTATT TCAATCTAACAGCCACCAATCTGAAATTGTATTTCAAATGTTGATTCTGTAGTTCTTTAAATAATAATGAAGCTCATCTTATACATTTTGCTTTCACCAA TTGATTCCTTCTTCTTTTAGCCCACTATTAAAACATTTCTTACTGAATGGTTCATGTAGGCTTGCTGAACAGCACGCATTACTTGCTTCCTGAAGAGTTC CCCCATTCATCCATTTGTCCCATTAGTTGCTGTGGATTATCAAGTTTTGAAGGAACTGTACATCCCAACAGACTGAAACATTCTAAGTGAAATGAGTATA ATCCAAGTAACTGGTGAACTTTGGAGGTTTGGAGCTTGAAGAGAATGGCTAAGAAGATTTGAATTATAGGGAGGGAACAGAAATCATACATGAAAAGGTT TTACTGAGAAGGGGAAAACCTTAGATAGAGGGACATGTGAAACAAAATCATTTGAAATTTTGATTCAGACATCCATTTCCAGTGGCAAACAGCAAAGCCT GAACCCATAAACCCAAATGATAGGTGAAGTTGGGTGGTTTTATCCAATGTCTCAAGCAAGCAATGTCTGGGAATATCATAGAGTAACAAGTGCTGGTCAG CCAAAGAAACATTCACTGCTGGTGAACCAATACCATAAGCATGTATTATCTAAGCACTTGATCAAGAAATATACATGTTGTACAAGCTCTCAATTTTGTT CATTTATTATCAAATTTTTAAAATACAAGTTTGGTATGTGATTTGGAAAAGATGCCTTCTGGATCTTAAGCCAGTTGTCAGTGGAGGTCCTCAGGGCTGC AAATGTCAAGACATAACCCTGTTCCTCACCATCATGATACCAGATACAGGTGAATACATAGGAACTATCTGCCTGTGTCCTCAATCTCCCTTCAAACAAG ATGCTGATTTGTAGGGTACTTGGCAGGTTAAATTAAACCAGAAGAGGTGACTTAATAAAAAAGGGAATGACATTTAGGGTATAAAGATCTCATAAGAAAT GTAATATGTAAATTATATCTTGCTTTATGTTGTAAAATATACATTGTTTGCGCTAGAATAGAAATGATTTCTTTTCAATAAAAAGAAAGAAGGACTCTA

refseq_PRRX1.F1 (forward primer)
refseq_PRRX1.R1 (reverse primer)
Single exon skipping

Frame 0

*FEREEGGGWDRWGRP*PPATGTFWSGNRRWAA AWTARATSTPCRRKRTSPSVTC*TWRKPGTWWRH RRMRTWARLAGACWSRRDSPAAATPRSRTMTS* TQKKKRRESSEGIGQPSIAASCRLWSVSLSGHT ILMLLCEKTLPAG*TSPRRECRCGFRTEEPSSAG MREPC*PIKTLPSSNPTQET*LLWSSPSYLVLL RDPPIISPGGQRLRTAPWLLILPHVPTIALHRA STWPTALPT*D*RPRNIVYRGTRCQQSTEEKK*L NRPKKKSKTIRHLSCSVISSSAGGKK*ITNKQT KQN*NIGTMAEKSRRGAK*KLVNKCSSSLWDTT TTCFCVCLFCFSFIHALLNVLQASSDRFSPPTPM IV*SFKKNYSSQRNYIYIYIYIYPE*LPLLSSL TCLNLSALPCPLPSSLYRSSRSFRKAKVFLKNL CWTDIIPFLIVSIFVGHGKVFPSASEKIVVHNIC SLDCLIQCNWLRLANSKH*SLHLSYRFKLEATD YITITTTPHPMQSINQSS*VKDICCL*MICCHR LFGRRNIFHLREEEKFL**HKE*VLKGMPTSLSC LKDSEMHTYIYTVYIYIFIYIFHIYI*YCKLKF AISQTIQKANYTPSPLFLSL***NIN*GSCCFS FSTRSFH*SHRIIDRAAV*EQVPFSH*GFK*IRN YLRL*KCP*VWSLSSGEMLIHLIYHGNCS*RE* GIPFSHLSVLHLAGI*KKGTCLRGIPKGFSKFQ HLKGNCGLFLFFNILK*SLVSRAGSQD*SSISFS LFPAMLL*LARWT*PTTLPCCASVYPFVKWD** YLPTSQGCCEALFICSFILSCILCMSSTL*PWE ERDYKSVQC*WNDTVPESLVF**ENATLLYILIT LYLEMRNRFIFSDLSKITSFDQRII*DT*NRFF *FIFSS*PA*F**FLVVSD*KTLDQFWSNMPTL *SE*QARIFGFKMHF*HTFVFPLAYQIELMVMLF QSNSHQSEIVFQMLIL*FFK***SSSYTFCFHQ LIPSSFSPLLKHFLLNGSCRLAEQHALLAS*RV PPFIHLSH*LLWIIKF*RNCTSQQTETF*VK*V* SK*LVNFGGLELEENG*EDLNYREGTEIIHEKV LLRRGKP*IEGHVKQNHLKF*FRHPFPVANSKA *THKPK**VKLGGFIQCLKQAMSGNIIE*QVLVS QRNIHCW*TNTISMYYLST*SRNIHVVQALNFV HLLSNF*NTSLVCDLEKMPSGS*ASCQWRSSGL QMSRHNPVPHHHDTRYR*IHRNYLPVSSISLQTR C*FVGYLAG*IKPEEVT**KRE*HLGYKDLIRN VICKLYLALCCKIYIVCARIEMISFQ*KERRTL

Frame 1

DSSGKRGVGGIGGGDHDLQLRARSGAATGAGRP LGQPGQPRHPAGEKELLRQSPARPGGSRGHGGG TGG*ERGRGWPEPAGVAGTHQRQRHPAAGQ*PAE LRRKKEEKAAKE*DNLQ*QPAAGFGACL*ADTL S*CFCARRPCPPGEPHRGESAGVVSEPKSQVPQ E*ESHASQ*KRFPPQILLRRRDCCGAAHRTSSCS ETHRLSLLGDSVSVQRHGYLFCHMCQQ*PCTGH QHGQQHCQPETEGQGI*FTEEPGANSQLRKKNN *TGLRRNQKP*DTYPALLFLHLLGGKSKLQTNKQ SRTKILGPWQRKAGEEQNEN*LTNVPPPSGIPP PLVSVCVYFVFLSFMLCLMYSRLLQIGSAHPPP *LYEVLKKTTAAKETIYIYIYIYIQNDCLYCPH* LV*TLVPYPVLFPVLFIEALGAFEKPKSF*RIC AGQT*FPFSLSPSLLVMVRFFHQPLKK*LCTTS AHWTV*SNVIGCVWLILSTKVYI*AIDLSLKLQI ISLSPPPLTLCNQSINHLKLKIFVVFE*FAVTD YLVEEIFFT*ERKRNFSSNTKSEF*KACPHLFR ALRIVRCTLIYILYIFIYLYIYFIYIYNIASLSL QFPKQYKKQITHPHHCSYLYSDETLIRDLAAFL FLHEVFIKATE*LIGQLFENRSHFHIRALNELE TI*GYKNVLEFGA*ALVKC*YI*SIMGIAVRESK EYHLVIYPFFT*QEYERKAHV*EEYLKVFLNSN I*KAIVGYFYFLIF*NKV*CLGLGARTDLPFLF LCSQPCFCNLPGGLDQLHYHAVPQFTHL*NGINN TYLPHRGVVRLYSFAPLFFPVFSVCPALCSHGR KGTIKVYNVNGMIRYLKALFSSKKMLPCCTYL* PCIWK*EIGLYFQISQKSHHLTKE*FKTHRTDFF NLYFHPDQLSSNNF*L*VIKKLWINFGQTCQLC SLSDRQGFLGLRCTFSTHLYFPWHIRLS*W*CY FNLTATNLKLYFKC*FCSSLNNNEAHLIHFAFTN *FLLLLAHY*NISY*MVHVGLLNSTHYLLPEEF PHSSICPISCCGLSSFEGTVHPNRLKHSK*NEY NPSNW*TLEVWSLKRMAKKI*IIGREQKSYMKRF Y*EGENLR*RDM*NKII*NFDSDIHFQWQTAKP EPINPNDR*SWVVLSNVSSKQCLGIS*SNKCWS AKETFTAGEPIP*ACII*ALDQEIYMLYKLSILF IYYQIFKIQVWYVIWKRCLLDLKPVVSGGPQGC KCQDITLFLTIMIPDTGEYIGTICLCPQSPFKQ DADL*GTWQVKLNQKR*LNKKGNDI*GIKIS*EM *YVNYILLYVVKYTLFALE*K*FLFNKKKEGL

Frame 2

IRAGRGGWVGSVGETMTSSYGHVLERQPALGGR LDSPGNLDTLQAKKNFSVSHLLDLEEAGDMVAA QADENVGEAGRSLLESPGLTSGSDTPQQDNDQL NSEEKKKRKQRRNRTTFNSSQLQALERVFERTHY PDAFVREDLARRVNLTEARVQVWFQNRRAKFRR NERAMLANKNASLLKSYSGDVTAVEQPIVPRPA PRPTDYLSWGTASPYSAMATYSATCANNSPAQGI NMANSIANLRLKAKEYSLQRNQVPTVN*GKKII KQA*EEIKNHKTPILLCYFFICWGEKVNYKQTN KAELKYWDHGREKQERSKMKIS*QMFLLPLGYHH HLFLCVFILFFFHSCFA*CTPGFFR*VQPTHPH DCMKF*KKLQQPKKLYIYIYIYISRMIASTVLI DLFEP*CLTLSSSQFSL*KL*ELSKSQSLSEESV LDRHNSLSHCLHLCWSW*GFSISL*KNSCAQHL LTGLSDPM*LAASG*F*ALKSTSKL*I*A*SYR LYHYHHHPSPYAINQSIILS*RYLLSLNDLLSQT IW*KKYFSPERGREISLVTQRVSSKRHAHISFV P*G**DAHLYIYCIYLYIYIYISYIYIILQA*V CNFPNNTKSKLHTLTTVLISIVMKH*LGILLLFF FYTKFSLKPQNN**GSCLRTGPIFTLGL*MN*K LFEAIKMSLSLEPELW*NADTSDLSWELQLERV RNTI*SSIRSSLSRNMKERHMFKRNT*RFF*IPT FKRQLWAIFIF*YFEIKFSV*GWEPGLIFHFFF FVPSHAFVTCQVDLTNYITMLCLSLPICKMGLI ILTYLTGVL*GSIHLLLYSFLYSLYVQHFVAMGG KGL*KCTMLME*YGT*KPCFLVRKCYLAVHTYN LVFGNEK*VYIFRSLKNHII*PKNNLRHIEQIF LIYIFILTSLVLIIFSCE*LKNFGSILVKHANFV V*VTGKDFWV*DALLAHICISLGISD*ANGDVI SI*QPPI*NCISNVDSVVL*IIMKLILYILLSP IDSFFF*PTIKTFLTEWFM*AC*TARITCFLKSS PIHPFVPLVAVDYQVLKELYIPTD*NILSEMSI IQVTGELWRFGA*REWLRRFEL*GGNRNHT*KG FTEKGKTLDRGTCETKSFEILIQTSISSGKQQSL NP*TQMIGEVGWFYPMSQASNVWEYHRVTSAGQ PKKHSLLVNQYHKHVLSKHLIKKYTCCTSSQFC SFIIKFLKYKFGM*FGKDAFWILSQLSVEVLRAA NVKT*PCSSPS*YQIQVNT*ELSACVLNLPSNK MLICRVLGRLN*TRRGDLIKKGMTFRV*RSHKK CNM*IISCFML*NIHCLR*NRNDFFSIKRKKDS

Sequence NM_006902

TGATTCGAGCGGGAAGAGGGGGGTGGGTGGGATCGGTGGGGGAGACCATGACCTCCAGCTACGGGCACGTTCTGGAGCGGCAACCGGCGCTGGGCGGCCG CTTGGACAGCCCGGGCAACCTCGACACCCTGCAGGCGAAAAAGAACTTCTCCGTCAGTCACCTGCTAGACCTGGAGGAAGCCGGGGACATGGTGGCGGCA CAGGCGGATGAGAACGTGGGCGAGGCTGGCCGGAGCCTGCTGGAGTCGCCGGGACTCACCAGCGGCAGCGACACCCCGCAGCAGGACAATGACCAGCTGA ACTCAGAAGAAAAAAAGAAGAGAAAGCAGCGAAGGAATAGGACAACCTTCAATAGCAGCCAGCTGCAGGCTTTGGAGCGTGTCTTTGAGCGGACACACTA TCCTGATGCTTTTGTGCGAGAAGACCTTGCCCGCCGGGTGAACCTCACCGAGGCGAGAGTGCAGGTGTGGTTTCAGAACCGAAGAGCCAAGTTCCGCAGG AATGAGAGAGCCATGCTAGCCAATAAAAACGCTTCCCTCCTCAAATCCTACTCAGGAGACGTGACTGCTGTGGAGCAGCCCATCGTACCTCGTCCTGCTC CGAGACCCACCGATTATCTCTCCTGGGGGACAGCGTCTCCGTACAGATCCTCGTCCCTCCCAAGATGTTGTTTACACGAGGGGCTTCATAACGGATTCTA ACGGAAGACACTGAAAAGCGCCATGGCTACTTATTCTGCCACATGTGCCAACAATAGCCCTGCACAGGGCATCAACATGGCCAACAGCATTGCCAACCTG AGACTGAAGGCCAAGGAATATAGTTTACAGAGGAACCAGGTGCCAACAGTCAACTGAGGAAAAAAAATAATTAAACAGGCCTAAGAAGAAATCAAAAACC ATAAGACACCTATCCTGCTCTGTTATTTCTTCATCTGCTGGGGGGAAAAAGTAAATTACAAACAAACAAACAAAGCAGAACTAAAATATTGGGACCATGG CAGAGAAAAGCAGGAGAGGAGCAAAATGAAAATTAGTTAACAAATGTTCCTCCTCCCTCTGGGATACCACCACCACTTGTTTCTGTGTGTGTTTATTTTG TTTTTCTTTCATTCATGCTTTGCTTAATGTACTCCAGGCTTCTTCAGATAGGTTCAGCCCACCCACCCCCATGATTGTATGAAGTTTTAAAAAAAACTAC AGCAGCCAAAGAAACTATATATATATATATATATATATATATCCAGAATGATTGCCTCTACTGTCCTCATTGACTTGTTTGAACCTTAGTGCCTTACCCT GTCCTCTTCCCAGTTCTCTTTATAGAAGCTCTAGGAGCTTTCGAAAAGCCAAAGTCTTTCTGAAGAATCTGTGCTGGACAGACATAATTCCCTTTCTCAT TGTCTCCATCTTTGTTGGTCATGGTAAGGTTTTTCCATCAGCCTCTGAAAAAATAGTTGTGCACAACATCTGCTCACTGGACTGTCTGATCCAATGTAAT TGGCTGCGTCTGGCTAATTCTAAGCACTAAAGTCTACATCTAAGCTATAGATTTAAGCTTGAAGCTACAGATTATATCACTATCACCACCACCCCTCACC CTATGCAATCAATCAATCAATCATCTTAAGTTAAAGATATTTGTTGTCTTTGAATGATTTGCTGTCACAGACTATTTGGTAGAAGAAATATTTTTCACCT GAGAGAGGAAGAGAAATTTCTCTAGTAACACAAAGAGTGAGTTCTAAAAGGCATGCCCACATCTCTTTCGTGCCTTAAGGATAGTGAGATGCACACTTAT ATATATACTGTATATATTTATATATTTATATATATATTTCATATATATATATAATATTGCAAGCTTAAGTTTGCAATTTCCCAAACAATACAAAAAGCAA ATTACACACCCTCACCACTGTTCTTATCTCTATAGTGATGAAACATTAATTAGGGATCTTGCTGCTTTTCTTTTTCTACACGAAGTTTTCATTAAAGCCA CAGAATAATTGATAGGGCAGCTGTTTGAGAACAGGTCCCATTTTCACATTAGGGCTTTAAATGAATTAGAAACTATTTGAGGCTATAAAAATGTCCTTGA GTTTGGAGCCTGAGCTCTGGTGAAATGCTGATACATCTGATCTATCATGGGAATTGCAGTTAGAGAGAGTAAGGAATACCATTTAGTCATCTATCCGTTC TTCACTTAGCAGGAATATGAAAGAAAGGCACATGTTTAAGAGGAATACCTAAAGGTTTTTCTAAATTCCAACATTTAAAAGGCAATTGTGGGCTATTTTT ATTTTTTAATATTTTGAAATAAAGTTTAGTGTCTAGGGCTGGGAGCCAGGACTGATCTTCCATTTCTTTTTCTTTGTTCCCAGCCATGCTTTTGTAACTT GCCAGGTGGACTTGACCAACTACATTACCATGCTGTGCCTCAGTTTACCCATTTGTAAAATGGGATTAATAATACTTACCTACCTCACAGGGGTGTTGTG AGGCTCTATTCATTTGCTCCTTTATTCTTTCCTGTATTCTCTGTATGTCCAGCACTTTGTAGCCATGGGAGGAAAGGGACTATAAAAGTGTACAATGTTA ATGGAATGATACGGTACCTGAAAGCCTTGTTTTCTAGTAAGAAAATGCTACCTTGCTGTACATACTTATAACCTTGTATTTGGAAATGAGAAATAGGTTT ATATTTTCAGATCTCTCAAAAATCACATCATTTGACCAAAGAATAATTTAAGACACATAGAACAGATTTTTTTAATTTATATTTTCATCCTGACCAGCTT AGTTCTAATAATTTTTAGTTGTGAGTGATTAAAAAACTTTGGATCAATTTTGGTCAAACATGCCAACTTTGTAGTCTGAGTGACAGGCAAGGATTTTTGG GTTTAAGATGCACTTTTAGCACACATTTGTATTTCCCTTGGCATATCAGATTGAGCTAATGGTGATGTTATTTCAATCTAACAGCCACCAATCTGAAATT GTATTTCAAATGTTGATTCTGTAGTTCTTTAAATAATAATGAAGCTCATCTTATACATTTTGCTTTCACCAATTGATTCCTTCTTCTTTTAGCCCACTAT TAAAACATTTCTTACTGAATGGTTCATGTAGGCTTGCTGAACAGCACGCATTACTTGCTTCCTGAAGAGTTCCCCCATTCATCCATTTGTCCCATTAGTT GCTGTGGATTATCAAGTTTTGAAGGAACTGTACATCCCAACAGACTGAAACATTCTAAGTGAAATGAGTATAATCCAAGTAACTGGTGAACTTTGGAGGT TTGGAGCTTGAAGAGAATGGCTAAGAAGATTTGAATTATAGGGAGGGAACAGAAATCATACATGAAAAGGTTTTACTGAGAAGGGGAAAACCTTAGATAG AGGGACATGTGAAACAAAATCATTTGAAATTTTGATTCAGACATCCATTTCCAGTGGCAAACAGCAAAGCCTGAACCCATAAACCCAAATGATAGGTGAA GTTGGGTGGTTTTATCCAATGTCTCAAGCAAGCAATGTCTGGGAATATCATAGAGTAACAAGTGCTGGTCAGCCAAAGAAACATTCACTGCTGGTGAACC AATACCATAAGCATGTATTATCTAAGCACTTGATCAAGAAATATACATGTTGTACAAGCTCTCAATTTTGTTCATTTATTATCAAATTTTTAAAATACAA GTTTGGTATGTGATTTGGAAAAGATGCCTTCTGGATCTTAAGCCAGTTGTCAGTGGAGGTCCTCAGGGCTGCAAATGTCAAGACATAACCCTGTTCCTCA CCATCATGATACCAGATACAGGTGAATACATAGGAACTATCTGCCTGTGTCCTCAATCTCCCTTCAAACAAGATGCTGATTTGTAGGGTACTTGGCAGGT TAAATTAAACCAGAAGAGGTGACTTAATAAAAAAGGGAATGACATTTAGGGTATAAAGATCTCATAAGAAATGTAATATGTAAATTATATCTTGCTTTAT GTTGTAAAATATACATTGTTTGCGCTAGAATAGAAATGATTTCTTTTCAATAAAAAGAAAGAAGGACTCTA

refseq_PRRX1.F1 (forward primer)
refseq_PRRX1.R1 (reverse primer)
Single exon skipping

Frame 0

*FEREEGGGWDRWGRP*PPATGTFWSGNRRWAA AWTARATSTPCRRKRTSPSVTC*TWRKPGTWWRH RRMRTWARLAGACWSRRDSPAAATPRSRTMTS* TQKKKRRESSEGIGQPSIAASCRLWSVSLSGHT ILMLLCEKTLPAG*TSPRRECRCGFRTEEPSSAG MREPC*PIKTLPSSNPTQET*LLWSSPSYLVLL RDPPIISPGGQRLRTDPRPSQDVVYTRGFITDS NGRH*KAPWLLILPHVPTIALHRASTWPTALPT* D*RPRNIVYRGTRCQQSTEEKK*LNRPKKKSKT IRHLSCSVISSSAGGKK*ITNKQTKQN*NIGTM AEKSRRGAK*KLVNKCSSSLWDTTTTCFCVCLFC FSFIHALLNVLQASSDRFSPPTPMIV*SFKKNY SSQRNYIYIYIYIYPE*LPLLSSLTCLNLSALP CPLPSSLYRSSRSFRKAKVFLKNLCWTDIIPFLI VSIFVGHGKVFPSASEKIVVHNICSLDCLIQCN WLRLANSKH*SLHLSYRFKLEATDYITITTTPH PMQSINQSS*VKDICCL*MICCHRLFGRRNIFHL REEEKFL**HKE*VLKGMPTSLSCLKDSEMHTY IYTVYIYIFIYIFHIYI*YCKLKFAISQTIQKA NYTPSPLFLSL***NIN*GSCCFSFSTRSFH*SH RIIDRAAV*EQVPFSH*GFK*IRNYLRL*KCP* VWSLSSGEMLIHLIYHGNCS*RE*GIPFSHLSV LHLAGI*KKGTCLRGIPKGFSKFQHLKGNCGLFL FFNILK*SLVSRAGSQD*SSISFSLFPAMLL*L ARWT*PTTLPCCASVYPFVKWD**YLPTSQGCC EALFICSFILSCILCMSSTL*PWEERDYKSVQC* WNDTVPESLVF**ENATLLYILITLYLEMRNRF IFSDLSKITSFDQRII*DT*NRFF*FIFSS*PA *F**FLVVSD*KTLDQFWSNMPTL*SE*QARIFG FKMHF*HTFVFPLAYQIELMVMLFQSNSHQSEI VFQMLIL*FFK***SSSYTFCFHQLIPSSFSPL LKHFLLNGSCRLAEQHALLAS*RVPPFIHLSH*L LWIIKF*RNCTSQQTETF*VK*V*SK*LVNFGG LELEENG*EDLNYREGTEIIHEKVLLRRGKP*I EGHVKQNHLKF*FRHPFPVANSKA*THKPK**VK LGGFIQCLKQAMSGNIIE*QVLVSQRNIHCW*T NTISMYYLST*SRNIHVVQALNFVHLLSNF*NT SLVCDLEKMPSGS*ASCQWRSSGLQMSRHNPVPH HHDTRYR*IHRNYLPVSSISLQTRC*FVGYLAG *IKPEEVT**KRE*HLGYKDLIRNVICKLYLAL CCKIYIVCARIEMISFQ*KERRTL

Frame 1

DSSGKRGVGGIGGGDHDLQLRARSGAATGAGRP LGQPGQPRHPAGEKELLRQSPARPGGSRGHGGG TGG*ERGRGWPEPAGVAGTHQRQRHPAAGQ*PAE LRRKKEEKAAKE*DNLQ*QPAAGFGACL*ADTL S*CFCARRPCPPGEPHRGESAGVVSEPKSQVPQ E*ESHASQ*KRFPPQILLRRRDCCGAAHRTSSCS ETHRLSLLGDSVSVQILVPPKMLFTRGAS*RIL TEDTEKRHGYLFCHMCQQ*PCTGHQHGQQHCQP ETEGQGI*FTEEPGANSQLRKKNN*TGLRRNQKP *DTYPALLFLHLLGGKSKLQTNKQSRTKILGPW QRKAGEEQNEN*LTNVPPPSGIPPPLVSVCVYF VFLSFMLCLMYSRLLQIGSAHPPP*LYEVLKKTT AAKETIYIYIYIYIQNDCLYCPH*LV*TLVPYP VLFPVLFIEALGAFEKPKSF*RICAGQT*FPFS LSPSLLVMVRFFHQPLKK*LCTTSAHWTV*SNVI GCVWLILSTKVYI*AIDLSLKLQIISLSPPPLT LCNQSINHLKLKIFVVFE*FAVTDYLVEEIFFT *ERKRNFSSNTKSEF*KACPHLFRALRIVRCTLI YILYIFIYLYIYFIYIYNIASLSLQFPKQYKKQ ITHPHHCSYLYSDETLIRDLAAFLFLHEVFIKA TE*LIGQLFENRSHFHIRALNELETI*GYKNVLE FGA*ALVKC*YI*SIMGIAVRESKEYHLVIYPF FT*QEYERKAHV*EEYLKVFLNSNI*KAIVGYF YFLIF*NKV*CLGLGARTDLPFLFLCSQPCFCNL PGGLDQLHYHAVPQFTHL*NGINNTYLPHRGVV RLYSFAPLFFPVFSVCPALCSHGRKGTIKVYNV NGMIRYLKALFSSKKMLPCCTYL*PCIWK*EIGL YFQISQKSHHLTKE*FKTHRTDFFNLYFHPDQL SSNNF*L*VIKKLWINFGQTCQLCSLSDRQGFL GLRCTFSTHLYFPWHIRLS*W*CYFNLTATNLKL YFKC*FCSSLNNNEAHLIHFAFTN*FLLLLAHY *NISY*MVHVGLLNSTHYLLPEEFPHSSICPIS CCGLSSFEGTVHPNRLKHSK*NEYNPSNW*TLEV WSLKRMAKKI*IIGREQKSYMKRFY*EGENLR* RDM*NKII*NFDSDIHFQWQTAKPEPINPNDR* SWVVLSNVSSKQCLGIS*SNKCWSAKETFTAGEP IP*ACII*ALDQEIYMLYKLSILFIYYQIFKIQ VWYVIWKRCLLDLKPVVSGGPQGCKCQDITLFL TIMIPDTGEYIGTICLCPQSPFKQDADL*GTWQV KLNQKR*LNKKGNDI*GIKIS*EM*YVNYILLY VVKYTLFALE*K*FLFNKKKEGL

Frame 2

IRAGRGGWVGSVGETMTSSYGHVLERQPALGGR LDSPGNLDTLQAKKNFSVSHLLDLEEAGDMVAA QADENVGEAGRSLLESPGLTSGSDTPQQDNDQL NSEEKKKRKQRRNRTTFNSSQLQALERVFERTHY PDAFVREDLARRVNLTEARVQVWFQNRRAKFRR NERAMLANKNASLLKSYSGDVTAVEQPIVPRPA PRPTDYLSWGTASPYRSSSLPRCCLHEGLHNGF* RKTLKSAMATYSATCANNSPAQGINMANSIANL RLKAKEYSLQRNQVPTVN*GKKIIKQA*EEIKN HKTPILLCYFFICWGEKVNYKQTNKAELKYWDHG REKQERSKMKIS*QMFLLPLGYHHHLFLCVFIL FFFHSCFA*CTPGFFR*VQPTHPHDCMKF*KKL QQPKKLYIYIYIYISRMIASTVLIDLFEP*CLTL SSSQFSL*KL*ELSKSQSLSEESVLDRHNSLSH CLHLCWSW*GFSISL*KNSCAQHLLTGLSDPM* LAASG*F*ALKSTSKL*I*A*SYRLYHYHHHPSP YAINQSIILS*RYLLSLNDLLSQTIW*KKYFSP ERGREISLVTQRVSSKRHAHISFVP*G**DAHL YIYCIYLYIYIYISYIYIILQA*VCNFPNNTKSK LHTLTTVLISIVMKH*LGILLLFFFYTKFSLKP QNN**GSCLRTGPIFTLGL*MN*KLFEAIKMSL SLEPELW*NADTSDLSWELQLERVRNTI*SSIRS SLSRNMKERHMFKRNT*RFF*IPTFKRQLWAIF IF*YFEIKFSV*GWEPGLIFHFFFFVPSHAFVT CQVDLTNYITMLCLSLPICKMGLIILTYLTGVL* GSIHLLLYSFLYSLYVQHFVAMGGKGL*KCTML ME*YGT*KPCFLVRKCYLAVHTYNLVFGNEK*V YIFRSLKNHII*PKNNLRHIEQIFLIYIFILTSL VLIIFSCE*LKNFGSILVKHANFVV*VTGKDFW V*DALLAHICISLGISD*ANGDVISI*QPPI*N CISNVDSVVL*IIMKLILYILLSPIDSFFF*PTI KTFLTEWFM*AC*TARITCFLKSSPIHPFVPLV AVDYQVLKELYIPTD*NILSEMSIIQVTGELWR FGA*REWLRRFEL*GGNRNHT*KGFTEKGKTLDR GTCETKSFEILIQTSISSGKQQSLNP*TQMIGE VGWFYPMSQASNVWEYHRVTSAGQPKKHSLLVN QYHKHVLSKHLIKKYTCCTSSQFCSFIIKFLKYK FGM*FGKDAFWILSQLSVEVLRAANVKT*PCSS PS*YQIQVNT*ELSACVLNLPSNKMLICRVLGR LN*TRRGDLIKKGMTFRV*RSHKKCNM*IISCFM L*NIHCLR*NRNDFFSIKRKKDS