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Sequence NM_006109
GCGACTCGTCCCGCCTTCTGGGGCACTAGTTTGACTTTGTGATTGGCTACTAGTATCAAGGAATCCCGGCGTGGACAGCGCGAGGAGAAAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAGG
GGTTTGATTTCCTCTGCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGA
CCTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAAAGTGGAGAAGATTCGCAGGAACTCC
GAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGCATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGAG
TTTTGACCAACCACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGAGAGATGATATAATTGAGAA
TGCACCAACTACACACACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTTGTGTGACTATAGTAAGAGGATTGCA
GTGGCTCTTGAAATTGGGGCTGACCTCCCATCTAATCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTAGCATTTTCC
TGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGGCTCCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAA
CCACCACTCAGAGAAGGAGTTCTGCTCCTACCTCCAATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAACTCTTTGCCAAGGGC
TATGAAGACTATCTGCAGTCCCCGCTTCAGCCACTGATGGACAATCTGGAATCTCAGACATATGAAGTGTTTGAAAAGGACCCCATCAAATACTCTCAGT
ACCAGCAGGCCATCTATAAATGTCTGCTAGACCGAGTACCAGAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACC
CCTGGTGAACGCTTCCCTGCGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGCCGTGGTGACGCTAGAGAAC
TGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGTAGTCTCATCAGACATGAGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTTC
TGGGCTCATTTGCTGACAATGAATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCCCGGGGAGTACACTTC
CTTTCTGGCTCCCATCTCTTCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAGGACCGTGACCCTGAGGCCCAGTTTGAGATGCCTTATGTG
GTACGGCTGCACAACTTCCACCAGCTCTCTGCACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGCA
CCTTGGAATTTCCTGTGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTATCAGGACATCACTCTGAGTATCCGTCCAGA
GACTCACTCTCCTGGGATGTTCTCATGGTTTCCCATCCTCTTCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATCTGTGTGCGTTTCTGG
CGATGCAGCAATTCCAAGAAGGTGTGGTATGAGTGGGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGCCGCTCATATACCATTGGCC
TCTAGCCCTGCGTGCCAAGTGTCCAGAGCCTTGGAAGCAGCTTCAGGTTCTGCTCCTGTAGTACAGAAGGTGCAGTACATCTATGGGCTGTGATTCCCCT
TGCCCATCAGAGAGGAGCATTTCAATCTGCTTTCCTGCCTTACATCAAGGTGGGCAAGGGATTATAATTAATTGCAGGGCTCAAGCCACCAATCTATGAA
GACCTCAGGCCAGGGGGTGAGGAATTAGTGCTGGATTTGAAGCTACGCACTCAGCCTCAAGAACTCCCTGGAATATCCCTGAGAACATGGGGTTTGAACG
GATTTTCAGCCTTTTTCTGTTCTTGTTTTGATGGTTTTGTGTAAGAGGAAATACAAATAAAGTTATAGCCCTTTACTGCACGACC
- refseq_PRMT5.F2 (forward primer)
- refseq_PRMT5.R2 (reverse primer)
- Alternative donor
Frame 0
ATRPAFWGTSLTL*LATSIKESRRGQREEKDGGDGGRGCWWEPRVQREGPELRPRNS*HTRGCGQAG
V*FPLHACLPSAFQEGVHSGTC*ESARSPDTIR
PTAVRKGLEYANCGKAFSMDSSRLKSGEDSQEL
RGGHVTGAEFWCIFGSSSFPAAP*SGR*HQPGQS
FDQPHPHWPSLFHVLDAGTLGGTRGPER*YN*E
CTNYTHRGVQWGGENVDVVAQLPDFV*L**EDC
SGS*NWG*PPI*SCH*SLAWGAHQSSHSPH*HFP
DQ*EGISCSF*DAPEAHLPAPQVGGAVHHHRHQ
PPLREGVLLLPPIPGILKPEPSST*CL*TLCQG
L*RLSAVPASATDGQSGISDI*SV*KGPHQILSV
PAGHL*MSARPSTRRGEGYQCPGTDGAGSRTGT
PGERFPAGSQAGRPADKAVCCGEKPKCRGDARE
LAV*RMGKPSDRSLIRHEGMGGSRESRHHCQ*AS
GLIC*Q*IVA*VPGWSPALPKR*WCEHPRGVHF
LSGSHLFLQAVQ*GPSL*GEGP*P*GPV*DALC
GTAAQLPPALCTPALFHLQPSQQRSYD*QQPLLH
LGISCGGEHSTTWLCRLL*DCALSGHHSEYPSR
DSLSWDVLMVSHPLPY*AAHNGT*RPNHLCAFL
AMQQFQEGVV*VGCDSTSLFCYS*PHRPLIYHWP
LALRAKCPEPWKQLQVLLL*YRRCSTSMGCDSP
CPSERSISICFPALHQGGQGIIINCRAQATNL*
RPQARG*GISAGFEATHSASRTPWNIPENMGFER
IFSLFLFLF*WFCVRGNTNKVIALYCTT
Frame 1
RLVPPSGALV*LCDWLLVSRNPGVDSARRKMAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQ
GFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSD
LLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNS
EAAMLQELNFGAYLGLPAFLLPLNQEDNTNLAR
VLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIEN
APTTHTEEYSGEEKTWMWWHNFRTLCDYSKRIA
VALEIGADLPSNHVIDRWLGEPIKAAILPTSIF
LTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTN
HHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKG
YEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQ
YQQAIYKCLLDRVPEEEKDTNVQVLMVLGAGRGP
LVNASLRAAKQADRRIKLYAVEKNPNAVVTLEN
WQFEEWGSQVTVVSSDMREWVAPEKADIIVSEL
LGSFADNELSPECLDGAQHFLKDDGVSIPGEYTS
FLAPISSSKLYNEVRACREKDRDPEAQFEMPYV
VRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYC
TLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPE
THSPGMFSWFPILFPIKQPITVREGQTICVRFW
RCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIG
L*PCVPSVQSLGSSFRFCSCSTEGAVHLWAVIPL
AHQRGAFQSAFLPYIKVGKGL*LIAGLKPPIYE
DLRPGGEELVLDLKLRTQPQELPGISLRTWGLN
GFSAFFCSCFDGFV*EEIQIKL*PFTAR
Frame 2
DSSRLLGH*FDFVIGY*YQGIPAWTARGERWRRWRSGVLVGAACPAGGT*IASPK*LTH*GLWPSR
GLISSACLSSIRVSRGSSFRNLLRIGPVPRHDQ
TYCCQEGTGIR*LWESFLHGFVQTQKWRRFAGTP
RRPCYRS*ILVHIWVFQLSCCPLIRKITPTWPE
F*PTTSTLAITLPCSGCGYPWWHQRT*EMI*LR
MHQLHTQRSTVGRRKRGCGGTTSGLCVTIVRGLQ
WLLKLGLTSHLIMSLIAGLGSPSKQPFSPLAFS
*PIRRDFLFFLRCTRGSSSGSSSWRCSSSSQAP
TTTQRRSSAPTSNTWNT*ARTVLHLMPMNSLPRA
MKTICSPRFSH*WTIWNLRHMKCLKRTPSNTLS
TSRPSINVC*TEYQKRRRIPMSRY*WCWEQDGD
PW*TLPCGQPSRPTGG*SCMLWRKTQMPW*R*RT
GSLKNGEAK*P*SHQT*GNGWLQRKQTSLSVSF
WAHLLTMNCRLSAWMEPSTS*KMMV*ASPGSTL
PFWLPSLPPSCTMRSEPVGRRTVTLRPSLRCLMW
YGCTTSTSSLHPSPVSPSAIPTEIL*LTTTAIA
PWNFLWR*TQYYMALPATLRLCFIRTSL*VSVQ
RLTLLGCSHGFPSSSLLSSP*RYVKAKPSVCVSG
DAAIPRRCGMSGL*QHQSVLLFITPQAAHIPLA
SSPACQVSRALEAASGSAPVVQKVQYIYGL*FP
LPIREEHFNLLSCLTSRWARDYN*LQGSSHQSMK
TSGQGVRN*CWI*SYALSLKNSLEYP*EHGV*T
DFQPFSVLVLMVLCKRKYK*SYSPLLHD
Sequence NM_001039619
GCGACTCGTCCCGCCTTCTGGGGCACTAGTTTGACTTTGTGATTGGCTACTAGTATCAAGGAATCCCGGCGTGGACAGCGCGAGGAGAAAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAGG
GGTGAGGGCCGGACCTCCACGAGCGGAATGCGGGGTCCGAACTCGGGGACGGAGAAGGGCAGACTAGTCATCCCGGAGAAGCAGGGGTTTGATTTCCTCT
GCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGACCTACTGCTGTCAGG
AAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAAAGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTA
CAGGAGCTGAATTTTGGTGCATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGAGTTTTGACCAACCACA
TCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGAGAGATGATATAATTGAGAATGCACCAACTACACA
CACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTTGTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAATT
GGGGCTGACCTCCCATCTAATCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTAGCATTTTCCTGACCAATAAGAAGG
GATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGGCTCCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAACCACCACTCAGAGAA
GGAGTTCTGCTCCTACCTCCAATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAACTCTTTGCCAAGGGCTATGAAGACTATCTG
CAGTCCCCGCTTCAGCCACTGATGGACAATCTGGAATCTCAGACATATGAAGTGTTTGAAAAGGACCCCATCAAATACTCTCAGTACCAGCAGGCCATCT
ATAAATGTCTGCTAGACCGAGTACCAGAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACCCCTGGTGAACGCTTC
CCTGCGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGCCGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAA
TGGGGAAGCCAAGTGACCGTAGTCTCATCAGACATGAGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTTCTGGGCTCATTTGCTG
ACAATGAATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCCCGGGGAGTACACTTCCTTTCTGGCTCCCAT
CTCTTCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAGGACCGTGACCCTGAGGCCCAGTTTGAGATGCCTTATGTGGTACGGCTGCACAAC
TTCCACCAGCTCTCTGCACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGCACCTTGGAATTTCCTG
TGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTATCAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGG
GATGTTCTCATGGTTTCCCATCCTCTTCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATCTGTGTGCGTTTCTGGCGATGCAGCAATTCC
AAGAAGGTGTGGTATGAGTGGGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGCCGCTCATATACCATTGGCCTCTAGCCCTGCGTGC
CAAGTGTCCAGAGCCTTGGAAGCAGCTTCAGGTTCTGCTCCTGTAGTACAGAAGGTGCAGTACATCTATGGGCTGTGATTCCCCTTGCCCATCAGAGAGG
AGCATTTCAATCTGCTTTCCTGCCTTACATCAAGGTGGGCAAGGGATTATAATTAATTGCAGGGCTCAAGCCACCAATCTATGAAGACCTCAGGCCAGGG
GGTGAGGAATTAGTGCTGGATTTGAAGCTACGCACTCAGCCTCAAGAACTCCCTGGAATATCCCTGAGAACATGGGGTTTGAACGGATTTTCAGCCTTTT
TCTGTTCTTGTTTTGATGGTTTTGTGTAAGAGGAAATACAAATAAAGTTATAGCCCTTTACTGCACGACC
- refseq_PRMT5.F2 (forward primer)
- refseq_PRMT5.R2 (reverse primer)
- Alternative donor
Frame 0
ATRPAFWGTSLTL*LATSIKESRRGQREEKDGGDGGRGCWWEPRVQREGPELRPRNS*HTRGCGQAG
VRAGPPRAECGVRTRGRRRAD*SSRRSRGLISS
ACLSSIRVSRGSSFRNLLRIGPVPRHDQTYCCQ
EGTGIR*LWESFLHGFVQTQKWRRFAGTPRRPCY
RS*ILVHIWVFQLSCCPLIRKITPTWPEF*PTT
STLAITLPCSGCGYPWWHQRT*EMI*LRMHQLH
TQRSTVGRRKRGCGGTTSGLCVTIVRGLQWLLKL
GLTSHLIMSLIAGLGSPSKQPFSPLAFS*PIRR
DFLFFLRCTRGSSSGSSSWRCSSSSQAPTTTQR
RSSAPTSNTWNT*ARTVLHLMPMNSLPRAMKTIC
SPRFSH*WTIWNLRHMKCLKRTPSNTLSTSRPS
INVC*TEYQKRRRIPMSRY*WCWEQDGDPW*TL
PCGQPSRPTGG*SCMLWRKTQMPW*R*RTGSLKN
GEAK*P*SHQT*GNGWLQRKQTSLSVSFWAHLL
TMNCRLSAWMEPSTS*KMMV*ASPGSTLPFWLP
SLPPSCTMRSEPVGRRTVTLRPSLRCLMWYGCTT
STSSLHPSPVSPSAIPTEIL*LTTTAIAPWNFL
WR*TQYYMALPATLRLCFIRTSL*VSVQRLTLL
GCSHGFPSSSLLSSP*RYVKAKPSVCVSGDAAIP
RRCGMSGL*QHQSVLLFITPQAAHIPLASSPAC
QVSRALEAASGSAPVVQKVQYIYGL*FPLPIRE
EHFNLLSCLTSRWARDYN*LQGSSHQSMKTSGQG
VRN*CWI*SYALSLKNSLEYP*EHGV*TDFQPF
SVLVLMVLCKRKYK*SYSPLLHD
Frame 1
RLVPPSGALV*LCDWLLVSRNPGVDSARRKMAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQ
G*GPDLHERNAGSELGDGEGQTSHPGEAGV*FPL
HACLPSAFQEGVHSGTC*ESARSPDTIRPTAVR
KGLEYANCGKAFSMDSSRLKSGEDSQELRGGHV
TGAEFWCIFGSSSFPAAP*SGR*HQPGQSFDQPH
PHWPSLFHVLDAGTLGGTRGPER*YN*ECTNYT
HRGVQWGGENVDVVAQLPDFV*L**EDCSGS*N
WG*PPI*SCH*SLAWGAHQSSHSPH*HFPDQ*EG
ISCSF*DAPEAHLPAPQVGGAVHHHRHQPPLRE
GVLLLPPIPGILKPEPSST*CL*TLCQGL*RLS
AVPASATDGQSGISDI*SV*KGPHQILSVPAGHL
*MSARPSTRRGEGYQCPGTDGAGSRTGTPGERF
PAGSQAGRPADKAVCCGEKPKCRGDARELAV*R
MGKPSDRSLIRHEGMGGSRESRHHCQ*ASGLIC*
Q*IVA*VPGWSPALPKR*WCEHPRGVHFLSGSH
LFLQAVQ*GPSL*GEGP*P*GPV*DALCGTAAQ
LPPALCTPALFHLQPSQQRSYD*QQPLLHLGISC
GGEHSTTWLCRLL*DCALSGHHSEYPSRDSLSW
DVLMVSHPLPY*AAHNGT*RPNHLCAFLAMQQF
QEGVV*VGCDSTSLFCYS*PHRPLIYHWPLALRA
KCPEPWKQLQVLLL*YRRCSTSMGCDSPCPSER
SISICFPALHQGGQGIIINCRAQATNL*RPQAR
G*GISAGFEATHSASRTPWNIPENMGFERIFSLF
LFLF*WFCVRGNTNKVIALYCTT
Frame 2
DSSRLLGH*FDFVIGY*YQGIPAWTARGERWRRWRSGVLVGAACPAGGT*IASPK*LTH*GLWPSR
GEGRTSTSGMRGPNSGTEKGRLVIPEKQGFDFL
CMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSG
RDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAML
QELNFGAYLGLPAFLLPLNQEDNTNLARVLTNH
IHTGHHSSMFWMRVPLVAPEDLRDDIIENAPTTH
TEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEI
GADLPSNHVIDRWLGEPIKAAILPTSIFLTNKK
GFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEK
EFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYL
QSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAI
YKCLLDRVPEEEKDTNVQVLMVLGAGRGPLVNAS
LRAAKQADRRIKLYAVEKNPNAVVTLENWQFEE
WGSQVTVVSSDMREWVAPEKADIIVSELLGSFA
DNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPI
SSSKLYNEVRACREKDRDPEAQFEMPYVVRLHN
FHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFP
VEVNTVLHGFAGYFETVLYQDITLSIRPETHSPG
MFSWFPILFPIKQPITVREGQTICVRFWRCSNS
KKVWYEWAVTAPVCSAIHNPTGRSYTIGL*PCV
PSVQSLGSSFRFCSCSTEGAVHLWAVIPLAHQRG
AFQSAFLPYIKVGKGL*LIAGLKPPIYEDLRPG
GEELVLDLKLRTQPQELPGISLRTWGLNGFSAF
FCSCFDGFV*EEIQIKL*PFTAR
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