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Sequences

Sequence NM_033154

TCCAGGTTTGTTCTGGAAAACACTAGGTCTGAGAGACAGCAACAACAAAAGTATACTCTACCATTAAAGAAATAATGCCGTATATGTAGTAGCACTTTTC AAAGAAGGAAGAAAGGTGGGAGTCATTTCCAGGGTAATTTTGACAAGAGGAACTGTCATTATGAACATGGTGGGTATGAACTTCTGCCTTCACGCTGCCA GGAGAATGATGGAAGCATGGAGATGAGGGATGTCCACAAGGACCGCCAACTAAGACACACTCCTTATAGAATCCAATGCAACAGGAAGCTAGTGAAATGA CATAGTGAAGATCAAATCCATATTACCATTTGGAGAAATAGAAAACCTCCAGAGAGAAAAATGAGGCGGAACACACAAGATGAAAACATGAGGAAATGGT TCAAGGTCACAATATGTATATTTGTCAGTCATTTTACTGCGCCCTACTCTGTGAAGAATAAGTTGAAGCCAGGCCAAATGGAGATGCTAAAGCTGACCAT GAACAAACGGTACAATGTCTCCCAGCAAGCTCTTGATCTCCAGAATCTCCGCTTTGACCCAGACTTGATGGGCCGTGACATTGATATAATCCTGAATCGA AGAAACTGCATGGCTGCCACCCTGAAGATCACTGAAAGAAATTTCCCTGAGCTGTTGTCTTTGAACTTGTGCAACAACAAACTGTACCAGCTGGATGGCC TTTCTGACATTACAGAGAAGGCTCCCAAAGTCAAGACCCTGAATCTCTCCAAAAATAAGCTGGAGTCGGCGTGGGAGTTGGGCAAGGTGAAAGGGCTGAA GCTCGAAGAGCTATGGCTAGAAGGGAACCCGTTGTGCAGCACCTTCTCGGACCAGTCTGCCTATGTAAGTGCCATCCGGGATTGTTTCCCCAAGTTGTTA CGCCTGGACGGCCGAGAGTTATCCGCACCAGTGATTGTTGACATTGACAGCTCTGAGACAATGAAACCCTGCAAGGAAAACTTTACTGGATCTGAGACCC TAAAGCATTTAGTCCTGCAATTCCTGCAGCAGAGCAACTTGTGCAAGTACTTCAAGGATAGCAGGAATATAAAAATTCTCAAAGACCCCTACCTGCAGAG GAAGCTGCTAAAGCACACAAAATGTCCACGAAACGTGGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCCTGGTGGACATGTGG TACCAGACCGTGAACACCTGCTTCCTCCCTCGGGCAGGCCCAGAGAGCCAGAGTGGAAGGATGGTCTCAGGGTTCTGATCTTGCCTTCATGTGGACCTTC ATCACTACCTCTGGCCGCAATTCCAGTCTGTGCATCATGAATGATAAGCTGTTTCTGAGGGATGCCAGCCCCCAAGAGACTCAGAGTGCCTTCTCCATCC CAGTGTCCACACTCTCCTCCAGCTCTGAGCCCTCCCTCTCCTAGGAGCACAGGAAATGGTGCAGGCTTTCTCTGCCCAGTCTGGGATGAAACTAGAGTGG TCTCAGAAGTGCCTTCAGGACAATAAGTGGAACTACATTAGAGCTGGTCAGGTCTTCACTATGCTCCAGACTGAGGGCAAGATCCCAGTGGAGGCCTTCA AGCAAATCCCCTAAAAGGAGCCCTTTGATGTCATCTTTGTCTTCCTTCTCATCCTCTTTGTTTCCTCTTTTCACCAGCCTAAGACCACTCTAAGGACTGG GGTTGGAGGCCTGGCTGACCAAGAAGCCAAAGTTAACTTGCAGGCCACAGGACATAGCCACCTGAAGAGCCAGTTGTTCTGTGTATTTGCCCCACTCATG ATCACCGTTTATTTTCATAATAAACAGTGACGTTACATGTTGTATGTT

refseq_NXF5.F1 (forward primer)
refseq_NXF5.R1 (reverse primer)
Multiple exon skipping

Frame 0

SRFVLENTRSERQQQQKYTLPLKK*CRICSSTF QRRKKGGSHFQGNFDKRNCHYEHGGYELLPSRCQ ENDGSMEMRDVHKDRQLRHTPYRIQCNRKLVK* HSEDQIHITIWRNRKPPERKMRRNTQDENMRKW FKVTICIFVSHFTAPYSVKNKLKPGQMEMLKLTM NKRYNVSQQALDLQNLRFDPDLMGRDIDIILNR RNCMAATLKITERNFPELLSLNLCNNKLYQLDG LSDITEKAPKVKTLNLSKNKLESAWELGKVKGLK LEELWLEGNPLCSTFSDQSAYVSAIRDCFPKLL RLDGRELSAPVIVDIDSSETMKPCKENFTGSET LKHLVLQFLQQSNLCKYFKDSRNIKILKDPYLQR KLLKHTKCPRNVDSLSALPETQHDFTSILVDMW YQTVNTCFLPRAGPESQSGRMVSGF*SCLHVDL HHYLWPQFQSVHHE**AVSEGCQPPRDSECLLHP SVHTLLQL*ALPLLGAQEMVQAFSAQSGMKLEW SQKCLQDNKWNYIRAGQVFTMLQTEGKIPVEAF KQIP*KEPFDVIFVFLLILFVSSFHQPKTTLRTG VGGLADQEAKVNLQATGHSHLKSQLFCVFAPLM ITVYFHNKQ*RYMLYV

Frame 1

PGLFWKTLGLRDSNNKSILYH*RNNAVYVVALF KEGRKVGVISRVILTRGTVIMNMVGMNFCLHAA RRMMEAWR*GMSTRTAN*DTLLIESNATGS**ND IVKIKSILPFGEIENLQREK*GGTHKMKT*GNG SRSQYVYLSVILLRPTL*RIS*SQAKWRC*S*P *TNGTMSPSKLLISRISALTQT*WAVTLI*S*IE ETAWLPP*RSLKEISLSCCL*TCATTNCTSWMA FLTLQRRLPKSRP*ISPKISWSRRGSWAR*KG* SSKSYG*KGTRCAAPSRTSLPM*VPSGIVSPSCY AWTAESYPHQ*LLTLTALRQ*NPARKTLLDLRP *SI*SCNSCSRATCASTSRIAGI*KFSKTPTCR GSC*STQNVHETWTPSVHCLKLSMTSPPSWWTCG TRP*TPASSLGQAQRARVEGWSQGSDLAFMWTF ITTSGRNSSLCIMNDKLFLRDASPQETQSAFSI PVSTLSSSSEPSLS*EHRKWCRLSLPSLG*N*SG LRSAFRTISGTTLELVRSSLCSRLRARSQWRPS SKSPKRSPLMSSLSSFSSSLFPLFTSLRPL*GL GLEAWLTKKPKLTCRPQDIAT*RASCSVYLPHS* SPFIFIINSDVTCCM

Frame 2

QVCSGKH*V*ETATTKVYSTIKEIMPYM**HFS KKEERWESFPG*F*QEELSL*TWWV*TSAFTLP GE*WKHGDEGCPQGPPTKTHSL*NPMQQEASEM T**RSNPYYHLEK*KTSREKNEAEHTR*KHEEMV QGHNMYICQSFYCALLCEE*VEARPNGDAKADH EQTVQCLPASS*SPESPL*PRLDGP*H*YNPES KKLHGCHPEDH*KKFP*AVVFELVQQQTVPAGWP F*HYREGSQSQDPESLQK*AGVGVGVGQGERAE ARRAMARREPVVQHLLGPVCLCKCHPGLFPQVV TPGRPRVIRTSDC*H*QL*DNETLQGKLYWI*DP KAFSPAIPAAEQLVQVLQG*QEYKNSQRPLPAE EAAKAHKMSTKRGLPQCIA*NSA*LHLHPGGHV VPDREHLLPPSGRPREPEWKDGLRVLILPSCGPS SLPLAAIPVCAS*MISCF*GMPAPKRLRVPSPS QCPHSPPALSPPSPRSTGNGAGFLCPVWDETRV VSEVPSGQ*VELH*SWSGLHYAPD*GQDPSGGLQ ANPLKGAL*CHLCLPSHPLCFLFSPA*DHSKDW GWRPG*PRSQS*LAGHRT*PPEEPVVLCICPTH DHRLFS**TVTLHVVC

Sequence NM_033155

TCCAGGTTTGTTCTGGAAAACACTAGGTCTGAGAGACAGCAACAACAAAAGTATACTCTACCATTAAAGAAATAATGCCGTATATGTAGTAGCACTTTTC AAAGAAGGAAGAAAGGTGGGAGTCATTTCCAGGGTAATTTTGACAAGAGGAACTGTCATTATGAACATGGTGGGTATGAACTTCTGCCTTCACGCTGCCA GGAGAATGATGGAAGCATGGAGATGAGGGATGTCCACAAGGACCGCCAACTAAGACACACTCCTTATAGAATCCAATGCAACAGGAAGCTAGTGAAATGA CATAGTGAAGATCAAATCCATATTACCATTTGGAGAAATAGAAAACCTCCAGAGAGAAAAATGAGGCGGAACACACAAGATGAAAACATGAGGAAATGGT TCAAGGTCACAATATGTATATTTGTCAGTCATTTTACTGCGCCCTACTCTGTGAAGAATAAGTTGAAGCCAGGCCAAATGGAGATGCTAAAGCTGACCAT GAACAAACGGTACAATGTCTCCCAGCAAGCTCTTGATCTCCAGAATCTCCGCTTTGACCCAGACTTGATGGGCCGTGACATTGATATAATCCTGAATCGA AGAAACTGCATGGCTGCCACCCTGAAGATCACTGAAAGAAATTTCCCTGAGCTGTTGTCTTTGAACTTGTGCAACAACAAACTGTACCAGCTGGATGGCC TTTCTGACATTACAGAGAAGGCTCCCAAAGTCAAGACCCTGAATCTCTCCAAAAATAAGCTGGAGTCGGCGTGGGAGTTGGGCAAGGTGAAAGGGCTGAA GCTCGAAGAGCTATGGCTAGAAGGGAACCCGTTGTGCAGCACCTTCTCGGACCAGTCTGCCTATGTAAGTGCCATCCGGGATTGTTTCCCCAAGTTGTTA CGCCTGGACGGCCGAGAGTTATCCGCACCAGTGATTGTTGACATTGACAGCTCTGAGACAATGAAACCCTGCAAGGAAAACTTTACTGGATCTGAGACCC TAAAGCATTTAGTCCTGCAATTCCTGCAGCAGAGCAACTTGTGCAAGTACTTCAAGGATAGCAGGAATATAAAAATTCTCAAAGACCCCTACCTGCAGAG GAAGCTGCTAAAGCACACAAAATGTCCACGAAACGTGGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCCTGGTGGACATGTGG TACCAGACCGTGAACACCTGCTTCCTCCCTCGGGCAGGCCCAGAGAGCCAGAGGTGGGTAGGAGGTTAAGGAGGATCCTGAGCACCTGAGCGCTTCCTTT TCAGGAAAGGATGCTCTGCTTTTCTGTCAACGGAGTGTTCAAGGAAGGTGGTGGTGTTTGTTGTCTCTGAAGTGGAAGGATGGTCTCAGGGTTCTGATCT TGCCTTCATGTGGACCTTCATCACTACCTCTGGCCGCAATTCCAGTCTGTGCATCATGAATGATAAGCTGTTTCTGAGGGATGCCAGCCCCCAAGAGACT CAGAGTGCCTTCTCCATCCCAGTGTCCACACTCTCCTCCAGCTCTGAGCCCTCCCTCTCCTAGGAGCACAGGAAATGGTGCAGGCTTTCTCTGCCCAGTC TGGGATGAAACTAGAGTGGTCTCAGAAGTGCCTTCAGGACAATAAGTGGAACTACATTAGAGCTGGTCAGGTCTTCACTATGCTCCAGACTGAGGGCAAG ATCCCAGTGGAGGCCTTCAAGCAAATCCCCTAAAAGGAGCCCTTTGATGTCATCTTTGTCTTCCTTCTCATCCTCTTTGTTTCCTCTTTTCACCAGCCTA AGACCACTCTAAGGACTGGGGTTGGAGGCCTGGCTGACCAAGAAGCCAAAGTTAACTTGCAGGCCACAGGACATAGCCACCTGAAGAGCCAGTTGTTCTG TGTATTTGCCCCACTCATGATCACCGTTTATTTTCATAATAAACAGTGACGTTACATGTTGTATGTT

refseq_NXF5.F1 (forward primer)
refseq_NXF5.R1 (reverse primer)
Multiple exon skipping

Frame 0

SRFVLENTRSERQQQQKYTLPLKK*CRICSSTF QRRKKGGSHFQGNFDKRNCHYEHGGYELLPSRCQ ENDGSMEMRDVHKDRQLRHTPYRIQCNRKLVK* HSEDQIHITIWRNRKPPERKMRRNTQDENMRKW FKVTICIFVSHFTAPYSVKNKLKPGQMEMLKLTM NKRYNVSQQALDLQNLRFDPDLMGRDIDIILNR RNCMAATLKITERNFPELLSLNLCNNKLYQLDG LSDITEKAPKVKTLNLSKNKLESAWELGKVKGLK LEELWLEGNPLCSTFSDQSAYVSAIRDCFPKLL RLDGRELSAPVIVDIDSSETMKPCKENFTGSET LKHLVLQFLQQSNLCKYFKDSRNIKILKDPYLQR KLLKHTKCPRNVDSLSALPETQHDFTSILVDMW YQTVNTCFLPRAGPESQRWVGG*GGS*APERFL FRKGCSAFLSTECSRKVVVFVVSEVEGWSQGSDL AFMWTFITTSGRNSSLCIMNDKLFLRDASPQET QSAFSIPVSTLSSSSEPSLS*EHRKWCRLSLPS LG*N*SGLRSAFRTISGTTLELVRSSLCSRLRAR SQWRPSSKSPKRSPLMSSLSSFSSSLFPLFTSL RPL*GLGLEAWLTKKPKLTCRPQDIAT*RASCS VYLPHS*SPFIFIINSDVTCCM

Frame 1

PGLFWKTLGLRDSNNKSILYH*RNNAVYVVALF KEGRKVGVISRVILTRGTVIMNMVGMNFCLHAA RRMMEAWR*GMSTRTAN*DTLLIESNATGS**ND IVKIKSILPFGEIENLQREK*GGTHKMKT*GNG SRSQYVYLSVILLRPTL*RIS*SQAKWRC*S*P *TNGTMSPSKLLISRISALTQT*WAVTLI*S*IE ETAWLPP*RSLKEISLSCCL*TCATTNCTSWMA FLTLQRRLPKSRP*ISPKISWSRRGSWAR*KG* SSKSYG*KGTRCAAPSRTSLPM*VPSGIVSPSCY AWTAESYPHQ*LLTLTALRQ*NPARKTLLDLRP *SI*SCNSCSRATCASTSRIAGI*KFSKTPTCR GSC*STQNVHETWTPSVHCLKLSMTSPPSWWTCG TRP*TPASSLGQAQRARGG*EVKEDPEHLSASF SGKDALLFCQRSVQGRWWCLLSLKWKDGLRVLI LPSCGPSSLPLAAIPVCAS*MISCF*GMPAPKRL RVPSPSQCPHSPPALSPPSPRSTGNGAGFLCPV WDETRVVSEVPSGQ*VELH*SWSGLHYAPD*GQ DPSGGLQANPLKGAL*CHLCLPSHPLCFLFSPA* DHSKDWGWRPG*PRSQS*LAGHRT*PPEEPVVL CICPTHDHRLFS**TVTLHVVC

Frame 2

QVCSGKH*V*ETATTKVYSTIKEIMPYM**HFS KKEERWESFPG*F*QEELSL*TWWV*TSAFTLP GE*WKHGDEGCPQGPPTKTHSL*NPMQQEASEM T**RSNPYYHLEK*KTSREKNEAEHTR*KHEEMV QGHNMYICQSFYCALLCEE*VEARPNGDAKADH EQTVQCLPASS*SPESPL*PRLDGP*H*YNPES KKLHGCHPEDH*KKFP*AVVFELVQQQTVPAGWP F*HYREGSQSQDPESLQK*AGVGVGVGQGERAE ARRAMARREPVVQHLLGPVCLCKCHPGLFPQVV TPGRPRVIRTSDC*H*QL*DNETLQGKLYWI*DP KAFSPAIPAAEQLVQVLQG*QEYKNSQRPLPAE EAAKAHKMSTKRGLPQCIA*NSA*LHLHPGGHV VPDREHLLPPSGRPREPEVGRRLRRILST*ALPF QERMLCFSVNGVFKEGGGVCCL*SGRMVSGF*S CLHVDLHHYLWPQFQSVHHE**AVSEGCQPPRD SECLLHPSVHTLLQL*ALPLLGAQEMVQAFSAQS GMKLEWSQKCLQDNKWNYIRAGQVFTMLQTEGK IPVEAFKQIP*KEPFDVIFVFLLILFVSSFHQP KTTLRTGVGGLADQEAKVNLQATGHSHLKSQLFC VFAPLMITVYFHNKQ*RYMLYV

Sequence NM_032946

TCCAGGTTTGTTCTGGAAAACACTAGGTCTGAGAGACAGCAACAACAAAAGTATACTCTACCATTAAAGAAATAATGCCGTATATGTAGTAGCACTTTTC AAAGAAGGAAGAAAGGTGGGAGTCATTTCCAGGGTAATTTTGACAAGAGGAACTGTCATTATGAACATGGTGGGTATGAACTTCTGCCTTCACGCTGCCA GGAGAATGATGGAAGCATGGAGATGAGGGATGTCCACAAGGACCGCCAACTAAGACACACTCCTTATAGAATCCAATGCAACAGGAAGCTAGTGAAATGA CATAGTGAAGATCAAATCCATATTACCATTTGGAGAAATAGAAAACCTCCAGAGAGAAAAATGAGGCGGAACACACAAGATGAAAACATGAGGAAATGGT TCAAGGTCACAATTCCTTATGGGATAAAGTATGACAAGGCATGGCTAATGAATTCAATCCAGAGCAATTGCAGTGTCCCCTTCACTCCGGTTGATTTCCA CTACATCCGAAATCGGGCATGCTTCTTTGTCCAGGTTGCTAGTGCTGCCTCTGCATTGAAGGATGTCAGTTATAAGATTTATGATGATGAGAACCAAAAG ATATGTATATTTGTCAGTCATTTTACTGCGCCCTACTCTGTGAAGAATAAGTTGAAGCCAGGCCAAATGGAGATGCTAAAGCTGACCATGAACAAACGGT ACAATGTCTCCCAGCAAGCTCTTGATCTCCAGAATCTCCGCTTTGACCCAGACTTGATGGGCCGTGACATTGATATAATCCTGAATCGAAGAAACTGCAT GGCTGCCACCCTGAAGATCACTGAAAGAAATTTCCCTGAGCTGTTGTCTTTGAACTTGTGCAACAACAAACTGTACCAGCTGGATGGCCTTTCTGACATT ACAGAGAAGGCTCCCAAAGTCAAGACCCTGAATCTCTCCAAAAATAAGCTGGAGTCGGCGTGGGAGTTGGGCAAGGTGAAAGGGCTGAAGCTCGAAGAGC TATGGCTAGAAGGGAACCCGTTGTGCAGCACCTTCTCGGACCAGTCTGCCTATGTAAGTGCCATCCGGGATTGTTTCCCCAAGTTGTTACGCCTGGACGG CCGAGAGTTATCCGCACCAGTGATTGTTGACATTGACAGCTCTGAGACAATGAAACCCTGCAAGGAAAACTTTACTGGATCTGAGACCCTAAAGCATTTA GTCCTGCAATTCCTGCAGCAGAGCAACTTGTGCAAGTACTTCAAGGATAGCAGGAATATAAAAATTCTCAAAGACCCCTACCTGCAGAGGAAGCTGCTAA AGCACACAAAATGTCCACGAAACGTGGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCCTGGTGGACATGTGGTACCAGACCGT GAACACCTGCTTCCTCCCTCGGGCAGGCCCAGAGAGCCAGAGGTGGTGGTGTTTGTTGTCTCTGAAGTGGAAGGATGGTCTCAGGGTTCTGATCTTGCCT TCATGTGGACCTTCATCACTACCTCTGGCCGCAATTCCAGTCTGTGCATCATGAATGATAAGCTGTTTCTGAGGGATGCCAGCCCCCAAGAGACTCAGAG TGCCTTCTCCATCCCAGTGTCCACACTCTCCTCCAGCTCTGAGCCCTCCCTCTCCTAGGAGCACAGGAAATGGTGCAGGCTTTCTCTGCCCAGTCTGGGA TGAAACTAGAGTGGTCTCAGAAGTGCCTTCAGGACAATAAGTGGAACTACATTAGAGCTGGTCAGGTCTTCACTATGCTCCAGACTGAGGGCAAGATCCC AGTGGAGGCCTTCAAGCAAATCCCCTAAAAGGAGCCCTTTGATGTCATCTTTGTCTTCCTTCTCATCCTCTTTGTTTCCTCTTTTCACCAGCCTAAGACC ACTCTAAGGACTGGGGTTGGAGGCCTGGCTGACCAAGAAGCCAAAGTTAACTTGCAGGCCACAGGACATAGCCACCTGAAGAGCCAGTTGTTCTGTGTAT TTGCCCCACTCATGATCACCGTTTATTTTCATAATAAACAGTGACGTTACATGTTGTATGTT

refseq_NXF5.F1 (forward primer)
refseq_NXF5.R1 (reverse primer)
Multiple exon skipping

Frame 0

SRFVLENTRSERQQQQKYTLPLKK*CRICSSTF QRRKKGGSHFQGNFDKRNCHYEHGGYELLPSRCQ ENDGSMEMRDVHKDRQLRHTPYRIQCNRKLVK* HSEDQIHITIWRNRKPPERKMRRNTQDENMRKW FKVTIPYGIKYDKAWLMNSIQSNCSVPFTPVDFH YIRNRACFFVQVASAASALKDVSYKIYDDENQK ICIFVSHFTAPYSVKNKLKPGQMEMLKLTMNKR YNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCM AATLKITERNFPELLSLNLCNNKLYQLDGLSDI TEKAPKVKTLNLSKNKLESAWELGKVKGLKLEE LWLEGNPLCSTFSDQSAYVSAIRDCFPKLLRLDG RELSAPVIVDIDSSETMKPCKENFTGSETLKHL VLQFLQQSNLCKYFKDSRNIKILKDPYLQRKLL KHTKCPRNVDSLSALPETQHDFTSILVDMWYQTV NTCFLPRAGPESQRWWCLLSLKWKDGLRVLILP SCGPSSLPLAAIPVCAS*MISCF*GMPAPKRLR VPSPSQCPHSPPALSPPSPRSTGNGAGFLCPVWD ETRVVSEVPSGQ*VELH*SWSGLHYAPD*GQDP SGGLQANPLKGAL*CHLCLPSHPLCFLFSPA*D HSKDWGWRPG*PRSQS*LAGHRT*PPEEPVVLCI CPTHDHRLFS**TVTLHVVC

Frame 1

PGLFWKTLGLRDSNNKSILYH*RNNAVYVVALF KEGRKVGVISRVILTRGTVIMNMVGMNFCLHAA RRMMEAWR*GMSTRTAN*DTLLIESNATGS**ND IVKIKSILPFGEIENLQREK*GGTHKMKT*GNG SRSQFLMG*SMTRHG**IQSRAIAVSPSLRLIS TTSEIGHASLSRLLVLPLH*RMSVIRFMMMRTKR YVYLSVILLRPTL*RIS*SQAKWRC*S*P*TNG TMSPSKLLISRISALTQT*WAVTLI*S*IEETA WLPP*RSLKEISLSCCL*TCATTNCTSWMAFLTL QRRLPKSRP*ISPKISWSRRGSWAR*KG*SSKS YG*KGTRCAAPSRTSLPM*VPSGIVSPSCYAWT AESYPHQ*LLTLTALRQ*NPARKTLLDLRP*SI* SCNSCSRATCASTSRIAGI*KFSKTPTCRGSC* STQNVHETWTPSVHCLKLSMTSPPSWWTCGTRP *TPASSLGQAQRARGGGVCCL*SGRMVSGF*SCL HVDLHHYLWPQFQSVHHE**AVSEGCQPPRDSE CLLHPSVHTLLQL*ALPLLGAQEMVQAFSAQSG MKLEWSQKCLQDNKWNYIRAGQVFTMLQTEGKIP VEAFKQIP*KEPFDVIFVFLLILFVSSFHQPKT TLRTGVGGLADQEAKVNLQATGHSHLKSQLFCV FAPLMITVYFHNKQ*RYMLYV

Frame 2

QVCSGKH*V*ETATTKVYSTIKEIMPYM**HFS KKEERWESFPG*F*QEELSL*TWWV*TSAFTLP GE*WKHGDEGCPQGPPTKTHSL*NPMQQEASEM T**RSNPYYHLEK*KTSREKNEAEHTR*KHEEMV QGHNSLWDKV*QGMANEFNPEQLQCPLHSG*FP LHPKSGMLLCPGC*CCLCIEGCQL*DL***EPK DMYICQSFYCALLCEE*VEARPNGDAKADHEQTV QCLPASS*SPESPL*PRLDGP*H*YNPESKKLH GCHPEDH*KKFP*AVVFELVQQQTVPAGWPF*H YREGSQSQDPESLQK*AGVGVGVGQGERAEARRA MARREPVVQHLLGPVCLCKCHPGLFPQVVTPGR PRVIRTSDC*H*QL*DNETLQGKLYWI*DPKAF SPAIPAAEQLVQVLQG*QEYKNSQRPLPAEEAAK AHKMSTKRGLPQCIA*NSA*LHLHPGGHVVPDR EHLLPPSGRPREPEVVVFVVSEVEGWSQGSDLA FMWTFITTSGRNSSLCIMNDKLFLRDASPQETQS AFSIPVSTLSSSSEPSLS*EHRKWCRLSLPSLG *N*SGLRSAFRTISGTTLELVRSSLCSRLRARS QWRPSSKSPKRSPLMSSLSSFSSSLFPLFTSLRP L*GLGLEAWLTKKPKLTCRPQDIAT*RASCSVY LPHS*SPFIFIINSDVTCCM

Sequence NM_033152

TCCAGGTTTGTTCTGGAAAACACTAGGTCTGAGAGACAGCAACAACAAAAGTATACTCTACCATTAAAGAAATAATGCCGTATATGTAGTAGCACTTTTC AAAGAAGGAAGAAAGGTGGGAGTCATTTCCAGGGTAATTTTGACAAGAGGAACTGTCATTATGAACATGGTGGGTATGAACTTCTGCCTTCACGCTGCCA GGAGAATGATGGAAGCATGGAGATGAGGGATGTCCACAAGGACCGCCAACTAAGACACACTCCTTATAGAATCCAATGCAACAGGAAGCTAGTGAAATGA CATAGTGAAGATCAAATCCATATTACCATTTGGAGAAATAGAAAACCTCCAGAGAGAAAAATGAGGCGGAACACACAAGATGAAAACATGAGGAAATGGT TCAAGGTCACAATTCCTTATGGGATAAAGTATGACAAGGCATGGCTAATGAATTCAATCCAGAGCAATTGCAGTGTCCCCTTCACTCCGGTTGATTTCCA CTACATCCGAAATCGGGCATGCTTCTTTGTCCAGGTTGCTAGTGCTGCCTCTGCATTGAAGGATGTCAGTTATAAGATTTATGATGATGAGAACCAAAAG ATATGTATATTTGTCAGTCATTTTACTGCGCCCTACTCTGTGAAGAATAAGTTGAAGCCAGGCCAAATGGAGATGCTAAAGCTGACCATGAACAAACGGT ACAATGTCTCCCAGCAAGCTCTTGATCTCCAGAATCTCCGCTTTGACCCAGACTTGATGGGCCGTGACATTGATATAATCCTGAATCGAAGAAACTGCAT GGCTGCCACCCTGAAGATCACTGAAAGAAATTTCCCTGAGCTGTTGTCTTTGAACTTGTGCAACAACAAACTGTACCAGCTGGATGGCCTTTCTGACATT ACAGAGAAGGCTCCCAAAGTCAAGACCCTGAATCTCTCCAAAAATAAGCTGGAGTCGGCGTGGGAGTTGGGCAAGGTGAAAGGGCTGAAGCTCGAAGAGC TATGGCTAGAAGGGAACCCGTTGTGCAGCACCTTCTCGGACCAGTCTGCCTATGTAAGTGCCATCCGGGATTGTTTCCCCAAGTTGTTACGCCTGGACGG CCGAGAGTTATCCGCACCAGTGATTGTTGACATTGACAGCTCTGAGACAATGAAACCCTGCAAGGAAAACTTTACTGGATCTGAGACCCTAAAGCATTTA GTCCTGCAATTCCTGCAGCAGAGCAACTTGTGCAAGTACTTCAAGGATAGCAGGAATATAAAAATTCTCAAAGACCCCTACCTGCAGAGGAAGCTGCTAA AGCACACAAAATGTCCACGAAACGTGGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCCTGGTGGACATGTGGTACCAGACCGT GAACACCTGCTTCCTCCCTCGGGCAGGCCCAGAGAGCCAGAGTGGAAGGATGGTCTCAGGGTTCTGATCTTGCCTTCATGTGGACCTTCATCACTACCTC TGGCCGCAATTCCAGTCTGTGCATCATGAATGATAAGCTGTTTCTGAGGGATGCCAGCCCCCAAGAGACTCAGAGTGCCTTCTCCATCCCAGTGTCCACA CTCTCCTCCAGCTCTGAGCCCTCCCTCTCCTAGGAGCACAGGAAATGGTGCAGGCTTTCTCTGCCCAGTCTGGGATGAAACTAGAGTGGTCTCAGAAGTG CCTTCAGGACAATAAGTGGAACTACATTAGAGCTGGTCAGGTCTTCACTATGCTCCAGACTGAGGGCAAGATCCCAGTGGAGGCCTTCAAGCAAATCCCC TAAAAGGAGCCCTTTGATGTCATCTTTGTCTTCCTTCTCATCCTCTTTGTTTCCTCTTTTCACCAGCCTAAGACCACTCTAAGGACTGGGGTTGGAGGCC TGGCTGACCAAGAAGCCAAAGTTAACTTGCAGGCCACAGGACATAGCCACCTGAAGAGCCAGTTGTTCTGTGTATTTGCCCCACTCATGATCACCGTTTA TTTTCATAATAAACAGTGACGTTACATGTTGTATGTT

refseq_NXF5.F1 (forward primer)
refseq_NXF5.R1 (reverse primer)
Multiple exon skipping

Frame 0

SRFVLENTRSERQQQQKYTLPLKK*CRICSSTF QRRKKGGSHFQGNFDKRNCHYEHGGYELLPSRCQ ENDGSMEMRDVHKDRQLRHTPYRIQCNRKLVK* HSEDQIHITIWRNRKPPERKMRRNTQDENMRKW FKVTIPYGIKYDKAWLMNSIQSNCSVPFTPVDFH YIRNRACFFVQVASAASALKDVSYKIYDDENQK ICIFVSHFTAPYSVKNKLKPGQMEMLKLTMNKR YNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCM AATLKITERNFPELLSLNLCNNKLYQLDGLSDI TEKAPKVKTLNLSKNKLESAWELGKVKGLKLEE LWLEGNPLCSTFSDQSAYVSAIRDCFPKLLRLDG RELSAPVIVDIDSSETMKPCKENFTGSETLKHL VLQFLQQSNLCKYFKDSRNIKILKDPYLQRKLL KHTKCPRNVDSLSALPETQHDFTSILVDMWYQTV NTCFLPRAGPESQSGRMVSGF*SCLHVDLHHYL WPQFQSVHHE**AVSEGCQPPRDSECLLHPSVH TLLQL*ALPLLGAQEMVQAFSAQSGMKLEWSQKC LQDNKWNYIRAGQVFTMLQTEGKIPVEAFKQIP *KEPFDVIFVFLLILFVSSFHQPKTTLRTGVGG LADQEAKVNLQATGHSHLKSQLFCVFAPLMITVY FHNKQ*RYMLYV

Frame 1

PGLFWKTLGLRDSNNKSILYH*RNNAVYVVALF KEGRKVGVISRVILTRGTVIMNMVGMNFCLHAA RRMMEAWR*GMSTRTAN*DTLLIESNATGS**ND IVKIKSILPFGEIENLQREK*GGTHKMKT*GNG SRSQFLMG*SMTRHG**IQSRAIAVSPSLRLIS TTSEIGHASLSRLLVLPLH*RMSVIRFMMMRTKR YVYLSVILLRPTL*RIS*SQAKWRC*S*P*TNG TMSPSKLLISRISALTQT*WAVTLI*S*IEETA WLPP*RSLKEISLSCCL*TCATTNCTSWMAFLTL QRRLPKSRP*ISPKISWSRRGSWAR*KG*SSKS YG*KGTRCAAPSRTSLPM*VPSGIVSPSCYAWT AESYPHQ*LLTLTALRQ*NPARKTLLDLRP*SI* SCNSCSRATCASTSRIAGI*KFSKTPTCRGSC* STQNVHETWTPSVHCLKLSMTSPPSWWTCGTRP *TPASSLGQAQRARVEGWSQGSDLAFMWTFITTS GRNSSLCIMNDKLFLRDASPQETQSAFSIPVST LSSSSEPSLS*EHRKWCRLSLPSLG*N*SGLRS AFRTISGTTLELVRSSLCSRLRARSQWRPSSKSP KRSPLMSSLSSFSSSLFPLFTSLRPL*GLGLEA WLTKKPKLTCRPQDIAT*RASCSVYLPHS*SPF IFIINSDVTCCM

Frame 2

QVCSGKH*V*ETATTKVYSTIKEIMPYM**HFS KKEERWESFPG*F*QEELSL*TWWV*TSAFTLP GE*WKHGDEGCPQGPPTKTHSL*NPMQQEASEM T**RSNPYYHLEK*KTSREKNEAEHTR*KHEEMV QGHNSLWDKV*QGMANEFNPEQLQCPLHSG*FP LHPKSGMLLCPGC*CCLCIEGCQL*DL***EPK DMYICQSFYCALLCEE*VEARPNGDAKADHEQTV QCLPASS*SPESPL*PRLDGP*H*YNPESKKLH GCHPEDH*KKFP*AVVFELVQQQTVPAGWPF*H YREGSQSQDPESLQK*AGVGVGVGQGERAEARRA MARREPVVQHLLGPVCLCKCHPGLFPQVVTPGR PRVIRTSDC*H*QL*DNETLQGKLYWI*DPKAF SPAIPAAEQLVQVLQG*QEYKNSQRPLPAEEAAK AHKMSTKRGLPQCIA*NSA*LHLHPGGHVVPDR EHLLPPSGRPREPEWKDGLRVLILPSCGPSSLP LAAIPVCAS*MISCF*GMPAPKRLRVPSPSQCPH SPPALSPPSPRSTGNGAGFLCPVWDETRVVSEV PSGQ*VELH*SWSGLHYAPD*GQDPSGGLQANP LKGAL*CHLCLPSHPLCFLFSPA*DHSKDWGWRP G*PRSQS*LAGHRT*PPEEPVVLCICPTHDHRL FS**TVTLHVVC

Sequence NM_033153

TCCAGGTTTGTTCTGGAAAACACTAGGTCTGAGAGACAGCAACAACAAAAGTATACTCTACCATTAAAGAAATAATGCCGTATATGTAGTAGCACTTTTC AAAGAAGGAAGAAAGGTGGGAGTCATTTCCAGGGTAATTTTGACAAGAGGAACTGTCATTATGAACATGGTGGGTATGAACTTCTGCCTTCACGCTGCCA GGAGAATGATGGAAGCATGGAGATGAGGGATGTCCACAAGGACCGCCAACTAAGACACACTCCTTATAGAATCCAATGCAACAGGAAGCTAGTGAAATGA CATAGTGAAGATCAAATCCATATTACCATTTGGAGAAATAGAAAACCTCCAGAGAGAAAAATGAGGCGGAACACACAAGATGAAAACATGAGGAAATGGT TCAAGGTCACAATTCCTTATGGGATAAAGTATGACAAGGCATGGCTAATGAATTCAATCCAGAGCAATTGCAGTGTCCCCTTCACTCCGGTTGATTTCCA CTACATCCGAAATCGGGCATGCTTCTTTGTCCAGGTTGCTAGTGCTGCCTCTGCATTGAAGGATGTCAGTTATAAGATTTATGATGATGAGAACCAAAAG ATATGTATATTTGTCAGTCATTTTACTGCGCCCTACTCTGTGAAGAATAAGTTGAAGCCAGGCCAAATGGAGATGCTAAAGCTGACCATGAACAAACGGT ACAATGTCTCCCAGCAAGCTCTTGATCTCCAGAATCTCCGCTTTGACCCAGACTTGATGGGCCGTGACATTGATATAATCCTGAATCGAAGAAACTGCAT GGCTGCCACCCTGAAGATCACTGAAAGAAATTTCCCTGAGAGAAGGCTCCCAAAGTCAAGACCCTGAATCTCTCCAAAAATAAGCTGGAGTCGGCGTGGG AGTTGGGCAAGGTGAAAGGGCTGAAGCTCGAAGAGCTATGGCTAGAAGGGAACCCGTTGTGCAGCACCTTCTCGGACCAGTCTGCCTATGTAAGTGCCAT CCGGGATTGTTTCCCCAAGTTGTTACGCCTGGACGGCCGAGAGTTATCCGCACCAGTGATTGTTGACATTGACAGCTCTGAGACAATGAAACCCTGCAAG GAAAACTTTACTGGATCTGAGACCCTAAAGCATTTAGTCCTGCAATTCCTGCAGCAGAGCAACTTGTGCAAGTACTTCAAGGATAGCAGGAATATAAAAA TTCTCAAAGACCCCTACCTGCAGAGGAAGCTGCTAAAGCACACAAAATGTCCACGAAACGTGGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTT CACCTCCATCCTGGTGGACATGTGGTACCAGACCGTGAACACCTGCTTCCTCCCTCGGGCAGGCCCAGAGAGCCAGAGTGGAAGGATGGTCTCAGGGTTC TGATCTTGCCTTCATGTGGACCTTCATCACTACCTCTGGCCGCAATTCCAGTCTGTGCATCATGAATGATAAGCTGTTTCTGAGGGATGCCAGCCCCCAA GAGACTCAGAGTGCCTTCTCCATCCCAGTGTCCACACTCTCCTCCAGCTCTGAGCCCTCCCTCTCCTAGGAGCACAGGAAATGGTGCAGGCTTTCTCTGC CCAGTCTGGGATGAAACTAGAGTGGTCTCAGAAGTGCCTTCAGGACAATAAGTGGAACTACATTAGAGCTGGTCAGGTCTTCACTATGCTCCAGACTGAG GGCAAGATCCCAGTGGAGGCCTTCAAGCAAATCCCCTAAAAGGAGCCCTTTGATGTCATCTTTGTCTTCCTTCTCATCCTCTTTGTTTCCTCTTTTCACC AGCCTAAGACCACTCTAAGGACTGGGGTTGGAGGCCTGGCTGACCAAGAAGCCAAAGTTAACTTGCAGGCCACAGGACATAGCCACCTGAAGAGCCAGTT GTTCTGTGTATTTGCCCCACTCATGATCACCGTTTATTTTCATAATAAACAGTGACGTTACATGTTGTATGTT

refseq_NXF5.F1 (forward primer)
refseq_NXF5.R1 (reverse primer)
Multiple exon skipping

Frame 0

SRFVLENTRSERQQQQKYTLPLKK*CRICSSTF QRRKKGGSHFQGNFDKRNCHYEHGGYELLPSRCQ ENDGSMEMRDVHKDRQLRHTPYRIQCNRKLVK* HSEDQIHITIWRNRKPPERKMRRNTQDENMRKW FKVTIPYGIKYDKAWLMNSIQSNCSVPFTPVDFH YIRNRACFFVQVASAASALKDVSYKIYDDENQK ICIFVSHFTAPYSVKNKLKPGQMEMLKLTMNKR YNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCM AATLKITERNFPERRLPKSRP*ISPKISWSRRG SWAR*KG*SSKSYG*KGTRCAAPSRTSLPM*VP SGIVSPSCYAWTAESYPHQ*LLTLTALRQ*NPAR KTLLDLRP*SI*SCNSCSRATCASTSRIAGI*K FSKTPTCRGSC*STQNVHETWTPSVHCLKLSMT SPPSWWTCGTRP*TPASSLGQAQRARVEGWSQGS DLAFMWTFITTSGRNSSLCIMNDKLFLRDASPQ ETQSAFSIPVSTLSSSSEPSLS*EHRKWCRLSL PSLG*N*SGLRSAFRTISGTTLELVRSSLCSRLR ARSQWRPSSKSPKRSPLMSSLSSFSSSLFPLFT SLRPL*GLGLEAWLTKKPKLTCRPQDIAT*RAS CSVYLPHS*SPFIFIINSDVTCCM

Frame 1

PGLFWKTLGLRDSNNKSILYH*RNNAVYVVALF KEGRKVGVISRVILTRGTVIMNMVGMNFCLHAA RRMMEAWR*GMSTRTAN*DTLLIESNATGS**ND IVKIKSILPFGEIENLQREK*GGTHKMKT*GNG SRSQFLMG*SMTRHG**IQSRAIAVSPSLRLIS TTSEIGHASLSRLLVLPLH*RMSVIRFMMMRTKR YVYLSVILLRPTL*RIS*SQAKWRC*S*P*TNG TMSPSKLLISRISALTQT*WAVTLI*S*IEETA WLPP*RSLKEISLREGSQSQDPESLQK*AGVGVG VGQGERAEARRAMARREPVVQHLLGPVCLCKCH PGLFPQVVTPGRPRVIRTSDC*H*QL*DNETLQ GKLYWI*DPKAFSPAIPAAEQLVQVLQG*QEYKN SQRPLPAEEAAKAHKMSTKRGLPQCIA*NSA*L HLHPGGHVVPDREHLLPPSGRPREPEWKDGLRV LILPSCGPSSLPLAAIPVCAS*MISCF*GMPAPK RLRVPSPSQCPHSPPALSPPSPRSTGNGAGFLC PVWDETRVVSEVPSGQ*VELH*SWSGLHYAPD* GQDPSGGLQANPLKGAL*CHLCLPSHPLCFLFSP A*DHSKDWGWRPG*PRSQS*LAGHRT*PPEEPV VLCICPTHDHRLFS**TVTLHVVC

Frame 2

QVCSGKH*V*ETATTKVYSTIKEIMPYM**HFS KKEERWESFPG*F*QEELSL*TWWV*TSAFTLP GE*WKHGDEGCPQGPPTKTHSL*NPMQQEASEM T**RSNPYYHLEK*KTSREKNEAEHTR*KHEEMV QGHNSLWDKV*QGMANEFNPEQLQCPLHSG*FP LHPKSGMLLCPGC*CCLCIEGCQL*DL***EPK DMYICQSFYCALLCEE*VEARPNGDAKADHEQTV QCLPASS*SPESPL*PRLDGP*H*YNPESKKLH GCHPEDH*KKFP*EKAPKVKTLNLSKNKLESAW ELGKVKGLKLEELWLEGNPLCSTFSDQSAYVSAI RDCFPKLLRLDGRELSAPVIVDIDSSETMKPCK ENFTGSETLKHLVLQFLQQSNLCKYFKDSRNIK ILKDPYLQRKLLKHTKCPRNVDSLSALPETQHDF TSILVDMWYQTVNTCFLPRAGPESQSGRMVSGF *SCLHVDLHHYLWPQFQSVHHE**AVSEGCQPP RDSECLLHPSVHTLLQL*ALPLLGAQEMVQAFSA QSGMKLEWSQKCLQDNKWNYIRAGQVFTMLQTE GKIPVEAFKQIP*KEPFDVIFVFLLILFVSSFH QPKTTLRTGVGGLADQEAKVNLQATGHSHLKSQL FCVFAPLMITVYFHNKQ*RYMLYV