Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence NM_001005745

GTTGTCATGGGGGAGGTGGTGGCGCTTGGTGGCCACTGGCGGCCGAGGTAGAGGCAGTGGCGCTTGAGTTGGTCGGGGGCAGCGGCAGATTTGAGGCTTA AGCAACTTCTTCCGGGGAAGAGTGCCAGTGCAGCCACTGTTACAATTCAAGATCTTGATCTATATCCATAGATTGGAATATTGGTGGGCCAGCAATCCTC AGACGCCTCACTTAGGACAAATGAGGAAACTGAGGCTTGGTGAAGTTACGAAACTTGTCCAAAATCACACAACTTGTAAAGGGCACAGCCAAGATTCAGA GCCAGGCTGTAAAAATTAAAATGAACAAATTACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGTCGTCCACATCAGTGGCAGAC AGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCGGTTAAGTACCTTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGAT GCTGTAAAAAGATTGAAAGCTACTGGAAAGAAAGCAGTTAAAGCAGTTCTGTGGGTCTCAGCAGATGGACTCAGAGTTGTGGATGAAAAAACTAAGGACC TCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGACAGGAACTTTGATAGAGCCTTTTCTTACATATGCCGTGATGGCACCACTCGTCG CTGGATCTGTCACTGCTTCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGTGCTTTTGCAGCCTGTTTAGAGCGCAAGCAGAAG CGGGAGAAGGAATGTGGAGTGACTGCTACTTTTGATGCTAGTCGGACCACTTTTACAAGAGAAGGATCATTCCGTGTCACAACAGCCACTGAACAAGCAG AAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAAACAGATAAGATAGTCGTTGGTTCATCAGTTGCCCCTGGCAACACTGCCCCATC CCCATCCTCTCCCACCTCTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCCATCCCACGCCGGCATGCTCCAATTGAACAGCTT GCTCGCCAAGGCTCTTTCCGAGGTTTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAACTATCCCTACGCATCAATGAGTTGCCTTCCACTA TGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTAGAAGGGGAGGCAGAGAGCATCAGCTCCCTGTGCTCACAGATCACCAATGCCTT CAGCACACCTGAGGACCCCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAATCTCCTACCTTCCAAGGGACCGAGTGGGGTCAA TCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCCGGTCATAGACGTACTCCCTCTGAGGCCGACCGATGGTTAGAAGAGGTGTCTAAGAGCGTCCGGG CTCAGCAGCCCCAGGCCTCAGCTGCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCACCCACTGCCATCTCCCAGCCAGCATCACCTTTCCAAGGGAATGC ATTCCTCACCTCTCAGCCTGTGCCAGTGGGTGTGGTCCCAGCCCTGCAACCAGCCTTTGTCCCTGCCCAGTCCTATCCTGTGGCCAATGGAATGCCCTAT CCAGCCCCTAATGTGCCTGTGGTGGGCATCACTCCCTCCCAGATGGTGGCCAACGTATTTGGCACTGCAGGCCACCCTCAGGCTGCCCATCCCCATCAGT CACCCAGCCTGGTCAGGCAGCAGACATTCCCTCACTACGAGGCAAGCAGTGCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAACGGTTC TGCAGCTTTCAATGGTGTAGATGATGGCAGGTTGGCCTCAGCAGACAGGCATACAGAGGTTCCTACAGGCACCTGCCCAGTGGATCCTTTTGAAGCCCAG TGGGCTGCATTAGAAAATAAGTCCAAGCAGCGTACTAATCCCTCCCCTACCAACCCTTTCTCCAGTGACTTACAGAAGACGTTTGAAATTGAACTTTAAG CAATCATTATGGCTATGTATCTTGTCCATACCAGACAGGGAGCAGGGGGTAGCGGTCAAAGGAGCAAAACAGACTTTGTCTCCTGATTAGTACTCTTTTC ACTAATCCCAAAGGTCCCAAGGAACAAGTCCAGGCCCAGAGTACTGTGAGGGGTGATTTTGAAAGACATGGGAAAAAGCATTCCTAGAGAAAAGCTGCCT TGCAATTAGGCTAAAGAAGTCAAGGAAATGTTGCTTTCTGTACTCCCTCTTCCCTTACCCCCTTACAAATCTCTGGCAACAGAGAGGCAAAGTATCTGAA CAAGAATCTATATTCCAAGCACATTTACTGAAATGTAAAACACAACAGGAAGCAAAGCAATCTCCCTTTGTTTTTCAGGCCATTCACCTGCCTCCTGTCA GTAGTGGCCTGTATTAGAGATCAAGAAGAGTGGTTTGTGCTCAGGCTGGGGAACAGAGAGGCACGCTATGCTGCCAGAATTCCCAGGAGGGCATATCAGC AACTGCCCAGCAGAGCTATATTTTGGGGGAGAAGTTGAGCTTCCATTTTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCTTTATTTTTA TGCATTTAGAATATTTTAAATAGTTCTCAGATATTAAGAAGTTGTATGAGTTGTAAGTAATCTTGCCAAAGGTAAAGGGGCTAGTTGTAAGAAATTGTAC ATAAGATTGATTTATCATTGATGCCTACTGAAATAAAAAGAGGAAAGGCTGGAAGCTGCAGACAGGATCCCTAGCTTGTTTTCTGTCAGTCATTCATTGT AAGTAGCACATTGCAACAACAATCATGCTTATGACCAATACAGTCACTAGGTTGTAGTTTTTTTTAAATAAAGGAAAAGCAGTATTGTCCTGGTTTTAAA CCTATGATGGAATTCTAATGTCATTATTTTAATGGAATCAATCGAAATATGCTCTATAGAGAATATATCTTTTATATATTGCTGCAGTTTCCTTATGTTA ATCCTTTAACACTAAGGTAACATGACATAATCATACCATAGAAGGGAACACAGGTTACCATATTGGTTTGTAATATGGGTCTTGGTGGGTTTTGTTTTAT CCTTTAAATTTTGTTCCCATGAGTTTTGTGGGGATGGGGATTCTGGTTTTATTAGCTTTGTGTGTGTCCTCTTCCCCCAAACCCCCTTTTGGTGAGAACA TCCCCTTGACAGTTGCAGCCTCTTGACCTCGGATAACAATAAGAGAGCTCATCTCATTTTTACTTTTGAACGTTGGCCTTACAATCAAATGTAAGTTATA TATATTTGTACTGATGAAAATTTATAATCTGCTTTAACAAAAATAAATGTTCATGGTAGAAGCTTTT

refseq_NUMB.F1 (forward primer)
refseq_NUMB.R1 (reverse primer)
Single exon skipping

Frame 0

VVMGEVVALGGHWRPR*RQWRLSWSGAAADLRL KQLLPGKSASAATVTIQDLDLYP*IGILVGQQSS DASLRTNEETEAW*SYETCPKSHNL*RAQPRFR ARL*KLK*TNYGKVLGERRMFMFQRPVVHISGR QMKKAFAPENVASRLSTLAM*KLMNQEECTSVKM L*KD*KLLERKQLKQFCGSQQMDSELWMKKLRT S*LTRR*RKFLSVPQTGTLIEPFLTYAVMAPLV AGSVTASWLSRTQVKG*AMQ*AVLLQPV*SASRS GRRNVE*LLLLMLVGPLLQEKDHSVSQQPLNKQ KERRS*NKCKMPRKLKQIR*SLVHQLPLATLPH PHPLPPLLLLMPRPLWR*TILMPSHAGMLQLNSL LAKALSEVFLLLARRCHPLNANYPYASMSCLPL CRGRLISPLKMQCQK*KGRQRASAPCAHRSPMP SAHLRTPSHLLR*PNQ*QWWHHNLLPSKGPSGVN LLVLPLQVSSRPVIDVLPLRPTDG*KRCLRASG LSSPRPQLLLCSQFSSLLHPLPSPSQHHLSKGM HSSPLSLCQWVWSQPCNQPLSLPSPILWPMECPI QPLMCLWWASLPPRWWPTYLALQATLRLPIPIS HPAWSGSRHSLTTRQAVLPPVPSLSLLLSTSTV LQLSMV*MMAGWPQQTGIQRFLQAPAQWILLKPS GLH*KISPSSVLIPPLPTLSPVTYRRRLKLNFK QSLWLCILSIPDREQGVAVKGAKQTLSPD*YSF H*SQRSQGTSPGPEYCEG*F*KTWEKAFLEKSCL AIRLKKSRKCCFLYSLFPYPLTNLWQQRGKVSE QESIFQAHLLKCKTQQEAKQSPFVFQAIHLPPV SSGLY*RSRRVVCAQAGEQRGTLCCQNSQEGISA TAQQSYILGEKLSFHFE*QNKYYIYQKPKSLFL CI*NILNSSQILRSCMSCK*SCQR*RG*L*EIV HKIDLSLMPTEIKRGKAGSCRQDP*LVFCQSFIV SSTLQQQSCL*PIQSLGCSFF*IKEKQYCPGFK PMMEF*CHYFNGINRNMLYREYIFYILLQFPYV NPLTLR*HDIIIP*KGTQVTILVCNMGLGGFCFI L*ILFP*VLWGWGFWFY*LCVCPLPPNPLLVRT SP*QLQPLDLG*Q*ESSSHFYF*TLALQSNVSY IYLY**KFIICFNKNKCSW*KL

Frame 1

LSWGRWWRLVATGGRGRGSGA*VGRGQRQI*GL SNFFRGRVPVQPLLQFKILIYIHRLEYWWASNP QTPHLGQMRKLRLGEVTKLVQNHTTCKGHSQDSE PGCKN*NEQITAKF*EKEGCLCSRGQSSTSVAD R*RRRSHRKM*LPG*VPWPCRS**IKRNAHL*R CCKKIESYWKESS*SSSVGLSRWTQSCG*KN*GP HS*PDDRESFFLCPRQEL**SLFLHMP*WHHSS LDLSLLHGCQGHR*KVEPCSRLCFCSLFRAQAE AGEGMWSDCYF*C*SDHFYKRRIIPCHNSH*TSR KRGDHETNARCQES*NR*DSRWFISCPWQHCPI PILSHLSYF*CHDLSGDEQSSCHPTPACSN*TA CSPRLFPRFSCS*PEDVTL*TPTIPTHQ*VAFHY AEED*FPH*KCSARSRRGGREHQLPVLTDHQCL QHT*GPLLICSDDQTSDSGGTTISYLPRDRVGS IFWCCLSRSLPGRS*TYSL*GRPMVRRGV*ERPG SAAPGLSCSSAASSPASSTHCHLPASITFPREC IPHLSACASGCGPSPATSLCPCPVLSCGQWNAL SSP*CACGGHHSLPDGGQRIWHCRPPSGCPSPSV TQPGQAADIPSLRGKQCYHQSLL*ASCSAPQRF CSFQWCR*WQVGLSRQAYRGSYRHLPSGSF*SP VGCIRK*VQAAY*SLPYQPFLQ*LTEDV*N*TLS NHYGYVSCPYQTGSRG*RSKEQNRLCLLISTLF TNPKGPKEQVQAQSTVRGDFERHGKKHS*RKAA LQLG*RSQGNVAFCTPSSLTPLQISGNREAKYLN KNLYSKHIY*NVKHNRKQSNLPLFFRPFTCLLS VVACIRDQEEWFVLRLGNREARYAARIPRRAYQ QLPSRAIFWGRS*ASILSNRINIIYIKSQNLYFY AFRIF*IVLRY*EVV*VVSNLAKGKGASCKKLY IRLIYH*CLLK*KEERLEAADRIPSLFSVSHSL *VAHCNNNHAYDQYSH*VVVFFK*RKSSIVLVLN L*WNSNVIILMESIEICSIENISFIYCCSFLML IL*H*GNMT*SYHRREHRLPYWFVIWVLVGFVL SFKFCSHEFCGDGDSGFISFVCVLFPQTPFW*EH PLDSCSLLTSDNNKRAHLIFTFERWPYNQM*VI YICTDENL*SALTKINVHGRSF

Frame 2

CHGGGGGAWWPLAAEVEAVALELVGGSGRFEA* ATSSGEECQCSHCYNSRS*SISIDWNIGGPAIL RRLT*DK*GN*GLVKLRNLSKITQLVKGTAKIQ SQAVKIKMNKLRQSFRRKKDVYVPEASRPHQWQT DEEGVRTGKCSFPVKYLGHVEVDESRGMHICED AVKRLKATGKKAVKAVLWVSADGLRVVDEKTKD LIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRR WICHCFMAVKDTGERLSHAVGCAFAACLERKQK REKECGVTATFDASRTTFTREGSFRVTTATEQA EREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPS PSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQL ARQGSFRGFPALSQKMSPFKRQLSLRINELPST MQRKTDFPIKNAVPEVEGEAESISSLCSQITNAF STPEDPFSSAPMTKPVTVVAPQSPTFQGTEWGQ SSGAASPGLFQAGHRRTPSEADRWLEEVSKSVR AQQPQASAAPLQPVLQPPPPTAISQPASPFQGNA FLTSQPVPVGVVPALQPAFVPAQSYPVANGMPY PAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQ SPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGS AAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQ WAALENKSKQRTNPSPTNPFSSDLQKTFEIEL* AIIMAMYLVHTRQGAGGSGQRSKTDFVS*LVLFS LIPKVPRNKSRPRVL*GVILKDMGKSIPREKLP CN*AKEVKEMLLSVLPLPLPPYKSLATERQSI* TRIYIPSTFTEM*NTTGSKAISLCFSGHSPASCQ *WPVLEIKKSGLCSGWGTERHAMLPEFPGGHIS NCPAELYFGGEVELPF*VTE*ILYISKAKIFIF MHLEYFK*FSDIKKLYEL*VILPKVKGLVVRNCT *D*FIIDAY*NKKRKGWKLQTGSLACFLSVIHC K*HIATTIMLMTNTVTRL*FFLNKGKAVLSWF* TYDGILMSLF*WNQSKYAL*RIYLLYIAAVSLC* SFNTKVT*HNHTIEGNTGYHIGL*YGSWWVLFY PLNFVPMSFVGMGILVLLALCVSSSPKPPFGEN IPLTVAAS*PRITIRELISFLLLNVGLTIKCKLY IFVLMKIYNLL*QK*MFMVEAF

Sequence NM_001005744

GTTGTCATGGGGGAGGTGGTGGCGCTTGGTGGCCACTGGCGGCCGAGGTAGAGGCAGTGGCGCTTGAGTTGGTCGGGGGCAGCGGCAGATTTGAGGCTTA AGCAACTTCTTCCGGGGAAGAGTGCCAGTGCAGCCACTGTTACAATTCAAGATCTTGATCTATATCCATAGATTGGAATATTGGTGGGCCAGCAATCCTC AGACGCCTCACTTAGGACAAATGAGGAAACTGAGGCTTGGTGAAGTTACGAAACTTGTCCAAAATCACACAACTTGTAAAGGGCACAGCCAAGATTCAGA GCCAGGCTGTAAAAATTAAAATGAACAAATTACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGTCGTCCACATCAGTGGCAGAC AGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCGGTTAAGTACCTTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGAT GCTGTAAAAAGATTGAAAGCTGAAAGGAAGTTCTTCAAAGGCTTCTTTGGAAAAACTGGAAAGAAAGCAGTTAAAGCAGTTCTGTGGGTCTCAGCAGATG GACTCAGAGTTGTGGATGAAAAAACTAAGGACCTCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGACAGGAACTTTGATAGAGCCTT TTCTTACATATGCCGTGATGGCACCACTCGTCGCTGGATCTGTCACTGCTTCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGT GCTTTTGCAGCCTGTTTAGAGCGCAAGCAGAAGCGGGAGAAGGAATGTGGAGTGACTGCTACTTTTGATGCTAGTCGGACCACTTTTACAAGAGAAGGAT CATTCCGTGTCACAACAGCCACTGAACAAGCAGAAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAAACAGATAAGATAGTCGTTGG TTCATCAGTTGCCCCTGGCAACACTGCCCCATCCCCATCCTCTCCCACCTCTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCC ATCCCACGCCGGCATGCTCCAATTGAACAGCTTGCTCGCCAAGGCTCTTTCCGAGGTTTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAAC TATCCCTACGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTAGAAGGGGAGGCAGAGAGCAT CAGCTCCCTGTGCTCACAGATCACCAATGCCTTCAGCACACCTGAGGACCCCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAA TCTCCTACCTTCCAAGGGACCGAGTGGGGTCAATCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCCGGTCATAGACGTACTCCCTCTGAGGCCGACC GATGGTTAGAAGAGGTGTCTAAGAGCGTCCGGGCTCAGCAGCCCCAGGCCTCAGCTGCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCACCCACTGCCAT CTCCCAGCCAGCATCACCTTTCCAAGGGAATGCATTCCTCACCTCTCAGCCTGTGCCAGTGGGTGTGGTCCCAGCCCTGCAACCAGCCTTTGTCCCTGCC CAGTCCTATCCTGTGGCCAATGGAATGCCCTATCCAGCCCCTAATGTGCCTGTGGTGGGCATCACTCCCTCCCAGATGGTGGCCAACGTATTTGGCACTG CAGGCCACCCTCAGGCTGCCCATCCCCATCAGTCACCCAGCCTGGTCAGGCAGCAGACATTCCCTCACTACGAGGCAAGCAGTGCTACCACCAGTCCCTT CTTTAAGCCTCCTGCTCAGCACCTCAACGGTTCTGCAGCTTTCAATGGTGTAGATGATGGCAGGTTGGCCTCAGCAGACAGGCATACAGAGGTTCCTACA GGCACCTGCCCAGTGGATCCTTTTGAAGCCCAGTGGGCTGCATTAGAAAATAAGTCCAAGCAGCGTACTAATCCCTCCCCTACCAACCCTTTCTCCAGTG ACTTACAGAAGACGTTTGAAATTGAACTTTAAGCAATCATTATGGCTATGTATCTTGTCCATACCAGACAGGGAGCAGGGGGTAGCGGTCAAAGGAGCAA AACAGACTTTGTCTCCTGATTAGTACTCTTTTCACTAATCCCAAAGGTCCCAAGGAACAAGTCCAGGCCCAGAGTACTGTGAGGGGTGATTTTGAAAGAC ATGGGAAAAAGCATTCCTAGAGAAAAGCTGCCTTGCAATTAGGCTAAAGAAGTCAAGGAAATGTTGCTTTCTGTACTCCCTCTTCCCTTACCCCCTTACA AATCTCTGGCAACAGAGAGGCAAAGTATCTGAACAAGAATCTATATTCCAAGCACATTTACTGAAATGTAAAACACAACAGGAAGCAAAGCAATCTCCCT TTGTTTTTCAGGCCATTCACCTGCCTCCTGTCAGTAGTGGCCTGTATTAGAGATCAAGAAGAGTGGTTTGTGCTCAGGCTGGGGAACAGAGAGGCACGCT ATGCTGCCAGAATTCCCAGGAGGGCATATCAGCAACTGCCCAGCAGAGCTATATTTTGGGGGAGAAGTTGAGCTTCCATTTTGAGTAACAGAATAAATAT TATATATATCAAAAGCCAAAATCTTTATTTTTATGCATTTAGAATATTTTAAATAGTTCTCAGATATTAAGAAGTTGTATGAGTTGTAAGTAATCTTGCC AAAGGTAAAGGGGCTAGTTGTAAGAAATTGTACATAAGATTGATTTATCATTGATGCCTACTGAAATAAAAAGAGGAAAGGCTGGAAGCTGCAGACAGGA TCCCTAGCTTGTTTTCTGTCAGTCATTCATTGTAAGTAGCACATTGCAACAACAATCATGCTTATGACCAATACAGTCACTAGGTTGTAGTTTTTTTTAA ATAAAGGAAAAGCAGTATTGTCCTGGTTTTAAACCTATGATGGAATTCTAATGTCATTATTTTAATGGAATCAATCGAAATATGCTCTATAGAGAATATA TCTTTTATATATTGCTGCAGTTTCCTTATGTTAATCCTTTAACACTAAGGTAACATGACATAATCATACCATAGAAGGGAACACAGGTTACCATATTGGT TTGTAATATGGGTCTTGGTGGGTTTTGTTTTATCCTTTAAATTTTGTTCCCATGAGTTTTGTGGGGATGGGGATTCTGGTTTTATTAGCTTTGTGTGTGT CCTCTTCCCCCAAACCCCCTTTTGGTGAGAACATCCCCTTGACAGTTGCAGCCTCTTGACCTCGGATAACAATAAGAGAGCTCATCTCATTTTTACTTTT GAACGTTGGCCTTACAATCAAATGTAAGTTATATATATTTGTACTGATGAAAATTTATAATCTGCTTTAACAAAAATAAATGTTCATGGTAGAAGCTTTT

refseq_NUMB.F1 (forward primer)
refseq_NUMB.R1 (reverse primer)
Single exon skipping

Frame 0

VVMGEVVALGGHWRPR*RQWRLSWSGAAADLRL KQLLPGKSASAATVTIQDLDLYP*IGILVGQQSS DASLRTNEETEAW*SYETCPKSHNL*RAQPRFR ARL*KLK*TNYGKVLGERRMFMFQRPVVHISGR QMKKAFAPENVASRLSTLAM*KLMNQEECTSVKM L*KD*KLKGSSSKASLEKLERKQLKQFCGSQQM DSELWMKKLRTS*LTRR*RKFLSVPQTGTLIEP FLTYAVMAPLVAGSVTASWLSRTQVKG*AMQ*AV LLQPV*SASRSGRRNVE*LLLLMLVGPLLQEKD HSVSQQPLNKQKERRS*NKCKMPRKLKQIR*SL VHQLPLATLPHPHPLPPLLLLMPRPLWR*TILMP SHAGMLQLNSLLAKALSEVFLLLARRCHPLNAN YPYASMSCLPLCRGRLISPLKMQCQK*KGRQRA SAPCAHRSPMPSAHLRTPSHLLR*PNQ*QWWHHN LLPSKGPSGVNLLVLPLQVSSRPVIDVLPLRPT DG*KRCLRASGLSSPRPQLLLCSQFSSLLHPLP SPSQHHLSKGMHSSPLSLCQWVWSQPCNQPLSLP SPILWPMECPIQPLMCLWWASLPPRWWPTYLAL QATLRLPIPISHPAWSGSRHSLTTRQAVLPPVP SLSLLLSTSTVLQLSMV*MMAGWPQQTGIQRFLQ APAQWILLKPSGLH*KISPSSVLIPPLPTLSPV TYRRRLKLNFKQSLWLCILSIPDREQGVAVKGA KQTLSPD*YSFH*SQRSQGTSPGPEYCEG*F*KT WEKAFLEKSCLAIRLKKSRKCCFLYSLFPYPLT NLWQQRGKVSEQESIFQAHLLKCKTQQEAKQSP FVFQAIHLPPVSSGLY*RSRRVVCAQAGEQRGTL CCQNSQEGISATAQQSYILGEKLSFHFE*QNKY YIYQKPKSLFLCI*NILNSSQILRSCMSCK*SC QR*RG*L*EIVHKIDLSLMPTEIKRGKAGSCRQD P*LVFCQSFIVSSTLQQQSCL*PIQSLGCSFF* IKEKQYCPGFKPMMEF*CHYFNGINRNMLYREY IFYILLQFPYVNPLTLR*HDIIIP*KGTQVTILV CNMGLGGFCFIL*ILFP*VLWGWGFWFY*LCVC PLPPNPLLVRTSP*QLQPLDLG*Q*ESSSHFYF *TLALQSNVSYIYLY**KFIICFNKNKCSW*KL

Frame 1

LSWGRWWRLVATGGRGRGSGA*VGRGQRQI*GL SNFFRGRVPVQPLLQFKILIYIHRLEYWWASNP QTPHLGQMRKLRLGEVTKLVQNHTTCKGHSQDSE PGCKN*NEQITAKF*EKEGCLCSRGQSSTSVAD R*RRRSHRKM*LPG*VPWPCRS**IKRNAHL*R CCKKIES*KEVLQRLLWKNWKESS*SSSVGLSRW TQSCG*KN*GPHS*PDDRESFFLCPRQEL**SL FLHMP*WHHSSLDLSLLHGCQGHR*KVEPCSRL CFCSLFRAQAEAGEGMWSDCYF*C*SDHFYKRRI IPCHNSH*TSRKRGDHETNARCQES*NR*DSRW FISCPWQHCPIPILSHLSYF*CHDLSGDEQSSC HPTPACSN*TACSPRLFPRFSCS*PEDVTL*TPT IPTHQ*VAFHYAEED*FPH*KCSARSRRGGREH QLPVLTDHQCLQHT*GPLLICSDDQTSDSGGTT ISYLPRDRVGSIFWCCLSRSLPGRS*TYSL*GRP MVRRGV*ERPGSAAPGLSCSSAASSPASSTHCH LPASITFPRECIPHLSACASGCGPSPATSLCPC PVLSCGQWNALSSP*CACGGHHSLPDGGQRIWHC RPPSGCPSPSVTQPGQAADIPSLRGKQCYHQSL L*ASCSAPQRFCSFQWCR*WQVGLSRQAYRGSY RHLPSGSF*SPVGCIRK*VQAAY*SLPYQPFLQ* LTEDV*N*TLSNHYGYVSCPYQTGSRG*RSKEQ NRLCLLISTLFTNPKGPKEQVQAQSTVRGDFER HGKKHS*RKAALQLG*RSQGNVAFCTPSSLTPLQ ISGNREAKYLNKNLYSKHIY*NVKHNRKQSNLP LFFRPFTCLLSVVACIRDQEEWFVLRLGNREAR YAARIPRRAYQQLPSRAIFWGRS*ASILSNRINI IYIKSQNLYFYAFRIF*IVLRY*EVV*VVSNLA KGKGASCKKLYIRLIYH*CLLK*KEERLEAADR IPSLFSVSHSL*VAHCNNNHAYDQYSH*VVVFFK *RKSSIVLVLNL*WNSNVIILMESIEICSIENI SFIYCCSFLMLIL*H*GNMT*SYHRREHRLPYW FVIWVLVGFVLSFKFCSHEFCGDGDSGFISFVCV LFPQTPFW*EHPLDSCSLLTSDNNKRAHLIFTF ERWPYNQM*VIYICTDENL*SALTKINVHGRSF

Frame 2

CHGGGGGAWWPLAAEVEAVALELVGGSGRFEA* ATSSGEECQCSHCYNSRS*SISIDWNIGGPAIL RRLT*DK*GN*GLVKLRNLSKITQLVKGTAKIQ SQAVKIKMNKLRQSFRRKKDVYVPEASRPHQWQT DEEGVRTGKCSFPVKYLGHVEVDESRGMHICED AVKRLKAERKFFKGFFGKTGKKAVKAVLWVSAD GLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAF SYICRDGTTRRWICHCFMAVKDTGERLSHAVGC AFAACLERKQKREKECGVTATFDASRTTFTREG SFRVTTATEQAEREEIMKQMQDAKKAETDKIVVG SSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHA IPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQ LSLRINELPSTMQRKTDFPIKNAVPEVEGEAESI SSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQ SPTFQGTEWGQSSGAASPGLFQAGHRRTPSEAD RWLEEVSKSVRAQQPQASAAPLQPVLQPPPPTAI SQPASPFQGNAFLTSQPVPVGVVPALQPAFVPA QSYPVANGMPYPAPNVPVVGITPSQMVANVFGT AGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPF FKPPAQHLNGSAAFNGVDDGRLASADRHTEVPT GTCPVDPFEAQWAALENKSKQRTNPSPTNPFSS DLQKTFEIEL*AIIMAMYLVHTRQGAGGSGQRSK TDFVS*LVLFSLIPKVPRNKSRPRVL*GVILKD MGKSIPREKLPCN*AKEVKEMLLSVLPLPLPPY KSLATERQSI*TRIYIPSTFTEM*NTTGSKAISL CFSGHSPASCQ*WPVLEIKKSGLCSGWGTERHA MLPEFPGGHISNCPAELYFGGEVELPF*VTE*I LYISKAKIFIFMHLEYFK*FSDIKKLYEL*VILP KVKGLVVRNCT*D*FIIDAY*NKKRKGWKLQTG SLACFLSVIHCK*HIATTIMLMTNTVTRL*FFL NKGKAVLSWF*TYDGILMSLF*WNQSKYAL*RIY LLYIAAVSLC*SFNTKVT*HNHTIEGNTGYHIG L*YGSWWVLFYPLNFVPMSFVGMGILVLLALCV SSSPKPPFGENIPLTVAAS*PRITIRELISFLLL NVGLTIKCKLYIFVLMKIYNLL*QK*MFMVEAF

Sequence NM_001005743

GTTGTCATGGGGGAGGTGGTGGCGCTTGGTGGCCACTGGCGGCCGAGGTAGAGGCAGTGGCGCTTGAGTTGGTCGGGGGCAGCGGCAGATTTGAGGCTTA AGCAACTTCTTCCGGGGAAGAGTGCCAGTGCAGCCACTGTTACAATTCAAGATCTTGATCTATATCCATAGATTGGAATATTGGTGGGCCAGCAATCCTC AGACGCCTCACTTAGGACAAATGAGGAAACTGAGGCTTGGTGAAGTTACGAAACTTGTCCAAAATCACACAACTTGTAAAGGGCACAGCCAAGATTCAGA GCCAGGCTGTAAAAATTAAAATGAACAAATTACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGTCGTCCACATCAGTGGCAGAC AGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCGGTTAAGTACCTTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGAT GCTGTAAAAAGATTGAAAGCTGAAAGGAAGTTCTTCAAAGGCTTCTTTGGAAAAACTGGAAAGAAAGCAGTTAAAGCAGTTCTGTGGGTCTCAGCAGATG GACTCAGAGTTGTGGATGAAAAAACTAAGGACCTCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGACAGGAACTTTGATAGAGCCTT TTCTTACATATGCCGTGATGGCACCACTCGTCGCTGGATCTGTCACTGCTTCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGT GCTTTTGCAGCCTGTTTAGAGCGCAAGCAGAAGCGGGAGAAGGAATGTGGAGTGACTGCTACTTTTGATGCTAGTCGGACCACTTTTACAAGAGAAGGAT CATTCCGTGTCACAACAGCCACTGAACAAGCAGAAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAAACAGATAAGATAGTCGTTGG TTCATCAGTTGCCCCTGGCAACACTGCCCCATCCCCATCCTCTCCCACCTCTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCC ATCCCACGCCGGCATGCTCCAATTGAACAGCTTGCTCGCCAAGGCTCTTTCCGAGGTTTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAAC TATCCCTACGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTAGAAGGGGAGGCAGAGAGCAT CAGCTCCCTGTGCTCACAGATCACCAATGCCTTCAGCACACCTGAGGACCCCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAA TCTCCTACCTTCCAAGCTAATGGCACTGACTCAGCCTTCCATGTGCTTGCTAAGCCAGCCCATACTGCTCTAGCACCCGTAGCAATGCCTGTGCGTGAAA CCAACCCTTGGGCCCATGCCCCTGATGCTGCTAACAAGGAAATTGCAGCCACATGTTCGGGGACCGAGTGGGGTCAATCTTCTGGTGCTGCCTCTCCAGG TCTCTTCCAGGCCGGTCATAGACGTACTCCCTCTGAGGCCGACCGATGGTTAGAAGAGGTGTCTAAGAGCGTCCGGGCTCAGCAGCCCCAGGCCTCAGCT GCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCACCCACTGCCATCTCCCAGCCAGCATCACCTTTCCAAGGGAATGCATTCCTCACCTCTCAGCCTGTGC CAGTGGGTGTGGTCCCAGCCCTGCAACCAGCCTTTGTCCCTGCCCAGTCCTATCCTGTGGCCAATGGAATGCCCTATCCAGCCCCTAATGTGCCTGTGGT GGGCATCACTCCCTCCCAGATGGTGGCCAACGTATTTGGCACTGCAGGCCACCCTCAGGCTGCCCATCCCCATCAGTCACCCAGCCTGGTCAGGCAGCAG ACATTCCCTCACTACGAGGCAAGCAGTGCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAACGGTTCTGCAGCTTTCAATGGTGTAGATG ATGGCAGGTTGGCCTCAGCAGACAGGCATACAGAGGTTCCTACAGGCACCTGCCCAGTGGATCCTTTTGAAGCCCAGTGGGCTGCATTAGAAAATAAGTC CAAGCAGCGTACTAATCCCTCCCCTACCAACCCTTTCTCCAGTGACTTACAGAAGACGTTTGAAATTGAACTTTAAGCAATCATTATGGCTATGTATCTT GTCCATACCAGACAGGGAGCAGGGGGTAGCGGTCAAAGGAGCAAAACAGACTTTGTCTCCTGATTAGTACTCTTTTCACTAATCCCAAAGGTCCCAAGGA ACAAGTCCAGGCCCAGAGTACTGTGAGGGGTGATTTTGAAAGACATGGGAAAAAGCATTCCTAGAGAAAAGCTGCCTTGCAATTAGGCTAAAGAAGTCAA GGAAATGTTGCTTTCTGTACTCCCTCTTCCCTTACCCCCTTACAAATCTCTGGCAACAGAGAGGCAAAGTATCTGAACAAGAATCTATATTCCAAGCACA TTTACTGAAATGTAAAACACAACAGGAAGCAAAGCAATCTCCCTTTGTTTTTCAGGCCATTCACCTGCCTCCTGTCAGTAGTGGCCTGTATTAGAGATCA AGAAGAGTGGTTTGTGCTCAGGCTGGGGAACAGAGAGGCACGCTATGCTGCCAGAATTCCCAGGAGGGCATATCAGCAACTGCCCAGCAGAGCTATATTT TGGGGGAGAAGTTGAGCTTCCATTTTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCTTTATTTTTATGCATTTAGAATATTTTAAATAG TTCTCAGATATTAAGAAGTTGTATGAGTTGTAAGTAATCTTGCCAAAGGTAAAGGGGCTAGTTGTAAGAAATTGTACATAAGATTGATTTATCATTGATG CCTACTGAAATAAAAAGAGGAAAGGCTGGAAGCTGCAGACAGGATCCCTAGCTTGTTTTCTGTCAGTCATTCATTGTAAGTAGCACATTGCAACAACAAT CATGCTTATGACCAATACAGTCACTAGGTTGTAGTTTTTTTTAAATAAAGGAAAAGCAGTATTGTCCTGGTTTTAAACCTATGATGGAATTCTAATGTCA TTATTTTAATGGAATCAATCGAAATATGCTCTATAGAGAATATATCTTTTATATATTGCTGCAGTTTCCTTATGTTAATCCTTTAACACTAAGGTAACAT GACATAATCATACCATAGAAGGGAACACAGGTTACCATATTGGTTTGTAATATGGGTCTTGGTGGGTTTTGTTTTATCCTTTAAATTTTGTTCCCATGAG TTTTGTGGGGATGGGGATTCTGGTTTTATTAGCTTTGTGTGTGTCCTCTTCCCCCAAACCCCCTTTTGGTGAGAACATCCCCTTGACAGTTGCAGCCTCT TGACCTCGGATAACAATAAGAGAGCTCATCTCATTTTTACTTTTGAACGTTGGCCTTACAATCAAATGTAAGTTATATATATTTGTACTGATGAAAATTT ATAATCTGCTTTAACAAAAATAAATGTTCATGGTAGAAGCTTTT

refseq_NUMB.F1 (forward primer)
refseq_NUMB.R1 (reverse primer)
Single exon skipping

Frame 0

VVMGEVVALGGHWRPR*RQWRLSWSGAAADLRL KQLLPGKSASAATVTIQDLDLYP*IGILVGQQSS DASLRTNEETEAW*SYETCPKSHNL*RAQPRFR ARL*KLK*TNYGKVLGERRMFMFQRPVVHISGR QMKKAFAPENVASRLSTLAM*KLMNQEECTSVKM L*KD*KLKGSSSKASLEKLERKQLKQFCGSQQM DSELWMKKLRTS*LTRR*RKFLSVPQTGTLIEP FLTYAVMAPLVAGSVTASWLSRTQVKG*AMQ*AV LLQPV*SASRSGRRNVE*LLLLMLVGPLLQEKD HSVSQQPLNKQKERRS*NKCKMPRKLKQIR*SL VHQLPLATLPHPHPLPPLLLLMPRPLWR*TILMP SHAGMLQLNSLLAKALSEVFLLLARRCHPLNAN YPYASMSCLPLCRGRLISPLKMQCQK*KGRQRA SAPCAHRSPMPSAHLRTPSHLLR*PNQ*QWWHHN LLPSKLMALTQPSMCLLSQPILL*HP*QCLCVK PTLGPMPLMLLTRKLQPHVRGPSGVNLLVLPLQ VSSRPVIDVLPLRPTDG*KRCLRASGLSSPRPQL LLCSQFSSLLHPLPSPSQHHLSKGMHSSPLSLC QWVWSQPCNQPLSLPSPILWPMECPIQPLMCLW WASLPPRWWPTYLALQATLRLPIPISHPAWSGSR HSLTTRQAVLPPVPSLSLLLSTSTVLQLSMV*M MAGWPQQTGIQRFLQAPAQWILLKPSGLH*KIS PSSVLIPPLPTLSPVTYRRRLKLNFKQSLWLCIL SIPDREQGVAVKGAKQTLSPD*YSFH*SQRSQG TSPGPEYCEG*F*KTWEKAFLEKSCLAIRLKKS RKCCFLYSLFPYPLTNLWQQRGKVSEQESIFQAH LLKCKTQQEAKQSPFVFQAIHLPPVSSGLY*RS RRVVCAQAGEQRGTLCCQNSQEGISATAQQSYI LGEKLSFHFE*QNKYYIYQKPKSLFLCI*NILNS SQILRSCMSCK*SCQR*RG*L*EIVHKIDLSLM PTEIKRGKAGSCRQDP*LVFCQSFIVSSTLQQQ SCL*PIQSLGCSFF*IKEKQYCPGFKPMMEF*CH YFNGINRNMLYREYIFYILLQFPYVNPLTLR*H DIIIP*KGTQVTILVCNMGLGGFCFIL*ILFP* VLWGWGFWFY*LCVCPLPPNPLLVRTSP*QLQPL DLG*Q*ESSSHFYF*TLALQSNVSYIYLY**KF IICFNKNKCSW*KL

Frame 1

LSWGRWWRLVATGGRGRGSGA*VGRGQRQI*GL SNFFRGRVPVQPLLQFKILIYIHRLEYWWASNP QTPHLGQMRKLRLGEVTKLVQNHTTCKGHSQDSE PGCKN*NEQITAKF*EKEGCLCSRGQSSTSVAD R*RRRSHRKM*LPG*VPWPCRS**IKRNAHL*R CCKKIES*KEVLQRLLWKNWKESS*SSSVGLSRW TQSCG*KN*GPHS*PDDRESFFLCPRQEL**SL FLHMP*WHHSSLDLSLLHGCQGHR*KVEPCSRL CFCSLFRAQAEAGEGMWSDCYF*C*SDHFYKRRI IPCHNSH*TSRKRGDHETNARCQES*NR*DSRW FISCPWQHCPIPILSHLSYF*CHDLSGDEQSSC HPTPACSN*TACSPRLFPRFSCS*PEDVTL*TPT IPTHQ*VAFHYAEED*FPH*KCSARSRRGGREH QLPVLTDHQCLQHT*GPLLICSDDQTSDSGGTT ISYLPS*WH*LSLPCAC*ASPYCSSTRSNACA*N QPLGPCP*CC*QGNCSHMFGDRVGSIFWCCLSR SLPGRS*TYSL*GRPMVRRGV*ERPGSAAPGLS CSSAASSPASSTHCHLPASITFPRECIPHLSACA SGCGPSPATSLCPCPVLSCGQWNALSSP*CACG GHHSLPDGGQRIWHCRPPSGCPSPSVTQPGQAA DIPSLRGKQCYHQSLL*ASCSAPQRFCSFQWCR* WQVGLSRQAYRGSYRHLPSGSF*SPVGCIRK*V QAAY*SLPYQPFLQ*LTEDV*N*TLSNHYGYVS CPYQTGSRG*RSKEQNRLCLLISTLFTNPKGPKE QVQAQSTVRGDFERHGKKHS*RKAALQLG*RSQ GNVAFCTPSSLTPLQISGNREAKYLNKNLYSKH IY*NVKHNRKQSNLPLFFRPFTCLLSVVACIRDQ EEWFVLRLGNREARYAARIPRRAYQQLPSRAIF WGRS*ASILSNRINIIYIKSQNLYFYAFRIF*I VLRY*EVV*VVSNLAKGKGASCKKLYIRLIYH*C LLK*KEERLEAADRIPSLFSVSHSL*VAHCNNN HAYDQYSH*VVVFFK*RKSSIVLVLNL*WNSNV IILMESIEICSIENISFIYCCSFLMLIL*H*GNM T*SYHRREHRLPYWFVIWVLVGFVLSFKFCSHE FCGDGDSGFISFVCVLFPQTPFW*EHPLDSCSL LTSDNNKRAHLIFTFERWPYNQM*VIYICTDENL *SALTKINVHGRSF

Frame 2

CHGGGGGAWWPLAAEVEAVALELVGGSGRFEA* ATSSGEECQCSHCYNSRS*SISIDWNIGGPAIL RRLT*DK*GN*GLVKLRNLSKITQLVKGTAKIQ SQAVKIKMNKLRQSFRRKKDVYVPEASRPHQWQT DEEGVRTGKCSFPVKYLGHVEVDESRGMHICED AVKRLKAERKFFKGFFGKTGKKAVKAVLWVSAD GLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAF SYICRDGTTRRWICHCFMAVKDTGERLSHAVGC AFAACLERKQKREKECGVTATFDASRTTFTREG SFRVTTATEQAEREEIMKQMQDAKKAETDKIVVG SSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHA IPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQ LSLRINELPSTMQRKTDFPIKNAVPEVEGEAESI SSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQ SPTFQANGTDSAFHVLAKPAHTALAPVAMPVRE TNPWAHAPDAANKEIAATCSGTEWGQSSGAASPG LFQAGHRRTPSEADRWLEEVSKSVRAQQPQASA APLQPVLQPPPPTAISQPASPFQGNAFLTSQPV PVGVVPALQPAFVPAQSYPVANGMPYPAPNVPVV GITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQ TFPHYEASSATTSPFFKPPAQHLNGSAAFNGVD DGRLASADRHTEVPTGTCPVDPFEAQWAALENKS KQRTNPSPTNPFSSDLQKTFEIEL*AIIMAMYL VHTRQGAGGSGQRSKTDFVS*LVLFSLIPKVPR NKSRPRVL*GVILKDMGKSIPREKLPCN*AKEVK EMLLSVLPLPLPPYKSLATERQSI*TRIYIPST FTEM*NTTGSKAISLCFSGHSPASCQ*WPVLEI KKSGLCSGWGTERHAMLPEFPGGHISNCPAELYF GGEVELPF*VTE*ILYISKAKIFIFMHLEYFK* FSDIKKLYEL*VILPKVKGLVVRNCT*D*FIID AY*NKKRKGWKLQTGSLACFLSVIHCK*HIATTI MLMTNTVTRL*FFLNKGKAVLSWF*TYDGILMS LF*WNQSKYAL*RIYLLYIAAVSLC*SFNTKVT *HNHTIEGNTGYHIGL*YGSWWVLFYPLNFVPMS FVGMGILVLLALCVSSSPKPPFGENIPLTVAAS *PRITIRELISFLLLNVGLTIKCKLYIFVLMKI YNLL*QK*MFMVEAF

Sequence NM_003744

GTTGTCATGGGGGAGGTGGTGGCGCTTGGTGGCCACTGGCGGCCGAGGTAGAGGCAGTGGCGCTTGAGTTGGTCGGGGGCAGCGGCAGATTTGAGGCTTA AGCAACTTCTTCCGGGGAAGAGTGCCAGTGCAGCCACTGTTACAATTCAAGATCTTGATCTATATCCATAGATTGGAATATTGGTGGGCCAGCAATCCTC AGACGCCTCACTTAGGACAAATGAGGAAACTGAGGCTTGGTGAAGTTACGAAACTTGTCCAAAATCACACAACTTGTAAAGGGCACAGCCAAGATTCAGA GCCAGGCTGTAAAAATTAAAATGAACAAATTACGGCAAAGTTTTAGGAGAAAGAAGGATGTTTATGTTCCAGAGGCCAGTCGTCCACATCAGTGGCAGAC AGATGAAGAAGGCGTTCGCACCGGAAAATGTAGCTTCCCGGTTAAGTACCTTGGCCATGTAGAAGTTGATGAATCAAGAGGAATGCACATCTGTGAAGAT GCTGTAAAAAGATTGAAAGCTACTGGAAAGAAAGCAGTTAAAGCAGTTCTGTGGGTCTCAGCAGATGGACTCAGAGTTGTGGATGAAAAAACTAAGGACC TCATAGTTGACCAGACGATAGAGAAAGTTTCTTTCTGTGCCCCAGACAGGAACTTTGATAGAGCCTTTTCTTACATATGCCGTGATGGCACCACTCGTCG CTGGATCTGTCACTGCTTCATGGCTGTCAAGGACACAGGTGAAAGGTTGAGCCATGCAGTAGGCTGTGCTTTTGCAGCCTGTTTAGAGCGCAAGCAGAAG CGGGAGAAGGAATGTGGAGTGACTGCTACTTTTGATGCTAGTCGGACCACTTTTACAAGAGAAGGATCATTCCGTGTCACAACAGCCACTGAACAAGCAG AAAGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGCTGAAACAGATAAGATAGTCGTTGGTTCATCAGTTGCCCCTGGCAACACTGCCCCATC CCCATCCTCTCCCACCTCTCCTACTTCTGATGCCACGACCTCTCTGGAGATGAACAATCCTCATGCCATCCCACGCCGGCATGCTCCAATTGAACAGCTT GCTCGCCAAGGCTCTTTCCGAGGTTTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAACTATCCCTACGCATCAATGAGTTGCCTTCCACTA TGCAGAGGAAGACTGATTTCCCCATTAAAAATGCAGTGCCAGAAGTAGAAGGGGAGGCAGAGAGCATCAGCTCCCTGTGCTCACAGATCACCAATGCCTT CAGCACACCTGAGGACCCCTTCTCATCTGCTCCGATGACCAAACCAGTGACAGTGGTGGCACCACAATCTCCTACCTTCCAAGCTAATGGCACTGACTCA GCCTTCCATGTGCTTGCTAAGCCAGCCCATACTGCTCTAGCACCCGTAGCAATGCCTGTGCGTGAAACCAACCCTTGGGCCCATGCCCCTGATGCTGCTA ACAAGGAAATTGCAGCCACATGTTCGGGGACCGAGTGGGGTCAATCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCCGGTCATAGACGTACTCCCTC TGAGGCCGACCGATGGTTAGAAGAGGTGTCTAAGAGCGTCCGGGCTCAGCAGCCCCAGGCCTCAGCTGCTCCTCTGCAGCCAGTTCTCCAGCCTCCTCCA CCCACTGCCATCTCCCAGCCAGCATCACCTTTCCAAGGGAATGCATTCCTCACCTCTCAGCCTGTGCCAGTGGGTGTGGTCCCAGCCCTGCAACCAGCCT TTGTCCCTGCCCAGTCCTATCCTGTGGCCAATGGAATGCCCTATCCAGCCCCTAATGTGCCTGTGGTGGGCATCACTCCCTCCCAGATGGTGGCCAACGT ATTTGGCACTGCAGGCCACCCTCAGGCTGCCCATCCCCATCAGTCACCCAGCCTGGTCAGGCAGCAGACATTCCCTCACTACGAGGCAAGCAGTGCTACC ACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAACGGTTCTGCAGCTTTCAATGGTGTAGATGATGGCAGGTTGGCCTCAGCAGACAGGCATACAG AGGTTCCTACAGGCACCTGCCCAGTGGATCCTTTTGAAGCCCAGTGGGCTGCATTAGAAAATAAGTCCAAGCAGCGTACTAATCCCTCCCCTACCAACCC TTTCTCCAGTGACTTACAGAAGACGTTTGAAATTGAACTTTAAGCAATCATTATGGCTATGTATCTTGTCCATACCAGACAGGGAGCAGGGGGTAGCGGT CAAAGGAGCAAAACAGACTTTGTCTCCTGATTAGTACTCTTTTCACTAATCCCAAAGGTCCCAAGGAACAAGTCCAGGCCCAGAGTACTGTGAGGGGTGA TTTTGAAAGACATGGGAAAAAGCATTCCTAGAGAAAAGCTGCCTTGCAATTAGGCTAAAGAAGTCAAGGAAATGTTGCTTTCTGTACTCCCTCTTCCCTT ACCCCCTTACAAATCTCTGGCAACAGAGAGGCAAAGTATCTGAACAAGAATCTATATTCCAAGCACATTTACTGAAATGTAAAACACAACAGGAAGCAAA GCAATCTCCCTTTGTTTTTCAGGCCATTCACCTGCCTCCTGTCAGTAGTGGCCTGTATTAGAGATCAAGAAGAGTGGTTTGTGCTCAGGCTGGGGAACAG AGAGGCACGCTATGCTGCCAGAATTCCCAGGAGGGCATATCAGCAACTGCCCAGCAGAGCTATATTTTGGGGGAGAAGTTGAGCTTCCATTTTGAGTAAC AGAATAAATATTATATATATCAAAAGCCAAAATCTTTATTTTTATGCATTTAGAATATTTTAAATAGTTCTCAGATATTAAGAAGTTGTATGAGTTGTAA GTAATCTTGCCAAAGGTAAAGGGGCTAGTTGTAAGAAATTGTACATAAGATTGATTTATCATTGATGCCTACTGAAATAAAAAGAGGAAAGGCTGGAAGC TGCAGACAGGATCCCTAGCTTGTTTTCTGTCAGTCATTCATTGTAAGTAGCACATTGCAACAACAATCATGCTTATGACCAATACAGTCACTAGGTTGTA GTTTTTTTTAAATAAAGGAAAAGCAGTATTGTCCTGGTTTTAAACCTATGATGGAATTCTAATGTCATTATTTTAATGGAATCAATCGAAATATGCTCTA TAGAGAATATATCTTTTATATATTGCTGCAGTTTCCTTATGTTAATCCTTTAACACTAAGGTAACATGACATAATCATACCATAGAAGGGAACACAGGTT ACCATATTGGTTTGTAATATGGGTCTTGGTGGGTTTTGTTTTATCCTTTAAATTTTGTTCCCATGAGTTTTGTGGGGATGGGGATTCTGGTTTTATTAGC TTTGTGTGTGTCCTCTTCCCCCAAACCCCCTTTTGGTGAGAACATCCCCTTGACAGTTGCAGCCTCTTGACCTCGGATAACAATAAGAGAGCTCATCTCA TTTTTACTTTTGAACGTTGGCCTTACAATCAAATGTAAGTTATATATATTTGTACTGATGAAAATTTATAATCTGCTTTAACAAAAATAAATGTTCATGG TAGAAGCTTTT

refseq_NUMB.F1 (forward primer)
refseq_NUMB.R1 (reverse primer)
Single exon skipping

Frame 0

VVMGEVVALGGHWRPR*RQWRLSWSGAAADLRL KQLLPGKSASAATVTIQDLDLYP*IGILVGQQSS DASLRTNEETEAW*SYETCPKSHNL*RAQPRFR ARL*KLK*TNYGKVLGERRMFMFQRPVVHISGR QMKKAFAPENVASRLSTLAM*KLMNQEECTSVKM L*KD*KLLERKQLKQFCGSQQMDSELWMKKLRT S*LTRR*RKFLSVPQTGTLIEPFLTYAVMAPLV AGSVTASWLSRTQVKG*AMQ*AVLLQPV*SASRS GRRNVE*LLLLMLVGPLLQEKDHSVSQQPLNKQ KERRS*NKCKMPRKLKQIR*SLVHQLPLATLPH PHPLPPLLLLMPRPLWR*TILMPSHAGMLQLNSL LAKALSEVFLLLARRCHPLNANYPYASMSCLPL CRGRLISPLKMQCQK*KGRQRASAPCAHRSPMP SAHLRTPSHLLR*PNQ*QWWHHNLLPSKLMALTQ PSMCLLSQPILL*HP*QCLCVKPTLGPMPLMLL TRKLQPHVRGPSGVNLLVLPLQVSSRPVIDVLP LRPTDG*KRCLRASGLSSPRPQLLLCSQFSSLLH PLPSPSQHHLSKGMHSSPLSLCQWVWSQPCNQP LSLPSPILWPMECPIQPLMCLWWASLPPRWWPT YLALQATLRLPIPISHPAWSGSRHSLTTRQAVLP PVPSLSLLLSTSTVLQLSMV*MMAGWPQQTGIQ RFLQAPAQWILLKPSGLH*KISPSSVLIPPLPT LSPVTYRRRLKLNFKQSLWLCILSIPDREQGVAV KGAKQTLSPD*YSFH*SQRSQGTSPGPEYCEG* F*KTWEKAFLEKSCLAIRLKKSRKCCFLYSLFP YPLTNLWQQRGKVSEQESIFQAHLLKCKTQQEAK QSPFVFQAIHLPPVSSGLY*RSRRVVCAQAGEQ RGTLCCQNSQEGISATAQQSYILGEKLSFHFE* QNKYYIYQKPKSLFLCI*NILNSSQILRSCMSCK *SCQR*RG*L*EIVHKIDLSLMPTEIKRGKAGS CRQDP*LVFCQSFIVSSTLQQQSCL*PIQSLGC SFF*IKEKQYCPGFKPMMEF*CHYFNGINRNMLY REYIFYILLQFPYVNPLTLR*HDIIIP*KGTQV TILVCNMGLGGFCFIL*ILFP*VLWGWGFWFY* LCVCPLPPNPLLVRTSP*QLQPLDLG*Q*ESSSH FYF*TLALQSNVSYIYLY**KFIICFNKNKCSW *KL

Frame 1

LSWGRWWRLVATGGRGRGSGA*VGRGQRQI*GL SNFFRGRVPVQPLLQFKILIYIHRLEYWWASNP QTPHLGQMRKLRLGEVTKLVQNHTTCKGHSQDSE PGCKN*NEQITAKF*EKEGCLCSRGQSSTSVAD R*RRRSHRKM*LPG*VPWPCRS**IKRNAHL*R CCKKIESYWKESS*SSSVGLSRWTQSCG*KN*GP HS*PDDRESFFLCPRQEL**SLFLHMP*WHHSS LDLSLLHGCQGHR*KVEPCSRLCFCSLFRAQAE AGEGMWSDCYF*C*SDHFYKRRIIPCHNSH*TSR KRGDHETNARCQES*NR*DSRWFISCPWQHCPI PILSHLSYF*CHDLSGDEQSSCHPTPACSN*TA CSPRLFPRFSCS*PEDVTL*TPTIPTHQ*VAFHY AEED*FPH*KCSARSRRGGREHQLPVLTDHQCL QHT*GPLLICSDDQTSDSGGTTISYLPS*WH*L SLPCAC*ASPYCSSTRSNACA*NQPLGPCP*CC* QGNCSHMFGDRVGSIFWCCLSRSLPGRS*TYSL *GRPMVRRGV*ERPGSAAPGLSCSSAASSPASS THCHLPASITFPRECIPHLSACASGCGPSPATSL CPCPVLSCGQWNALSSP*CACGGHHSLPDGGQR IWHCRPPSGCPSPSVTQPGQAADIPSLRGKQCY HQSLL*ASCSAPQRFCSFQWCR*WQVGLSRQAYR GSYRHLPSGSF*SPVGCIRK*VQAAY*SLPYQP FLQ*LTEDV*N*TLSNHYGYVSCPYQTGSRG*R SKEQNRLCLLISTLFTNPKGPKEQVQAQSTVRGD FERHGKKHS*RKAALQLG*RSQGNVAFCTPSSL TPLQISGNREAKYLNKNLYSKHIY*NVKHNRKQ SNLPLFFRPFTCLLSVVACIRDQEEWFVLRLGNR EARYAARIPRRAYQQLPSRAIFWGRS*ASILSN RINIIYIKSQNLYFYAFRIF*IVLRY*EVV*VV SNLAKGKGASCKKLYIRLIYH*CLLK*KEERLEA ADRIPSLFSVSHSL*VAHCNNNHAYDQYSH*VV VFFK*RKSSIVLVLNL*WNSNVIILMESIEICS IENISFIYCCSFLMLIL*H*GNMT*SYHRREHRL PYWFVIWVLVGFVLSFKFCSHEFCGDGDSGFIS FVCVLFPQTPFW*EHPLDSCSLLTSDNNKRAHL IFTFERWPYNQM*VIYICTDENL*SALTKINVHG RSF

Frame 2

CHGGGGGAWWPLAAEVEAVALELVGGSGRFEA* ATSSGEECQCSHCYNSRS*SISIDWNIGGPAIL RRLT*DK*GN*GLVKLRNLSKITQLVKGTAKIQ SQAVKIKMNKLRQSFRRKKDVYVPEASRPHQWQT DEEGVRTGKCSFPVKYLGHVEVDESRGMHICED AVKRLKATGKKAVKAVLWVSADGLRVVDEKTKD LIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRR WICHCFMAVKDTGERLSHAVGCAFAACLERKQK REKECGVTATFDASRTTFTREGSFRVTTATEQA EREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPS PSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQL ARQGSFRGFPALSQKMSPFKRQLSLRINELPST MQRKTDFPIKNAVPEVEGEAESISSLCSQITNAF STPEDPFSSAPMTKPVTVVAPQSPTFQANGTDS AFHVLAKPAHTALAPVAMPVRETNPWAHAPDAA NKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPS EADRWLEEVSKSVRAQQPQASAAPLQPVLQPPP PTAISQPASPFQGNAFLTSQPVPVGVVPALQPA FVPAQSYPVANGMPYPAPNVPVVGITPSQMVANV FGTAGHPQAAHPHQSPSLVRQQTFPHYEASSAT TSPFFKPPAQHLNGSAAFNGVDDGRLASADRHT EVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNP FSSDLQKTFEIEL*AIIMAMYLVHTRQGAGGSG QRSKTDFVS*LVLFSLIPKVPRNKSRPRVL*GV ILKDMGKSIPREKLPCN*AKEVKEMLLSVLPLPL PPYKSLATERQSI*TRIYIPSTFTEM*NTTGSK AISLCFSGHSPASCQ*WPVLEIKKSGLCSGWGT ERHAMLPEFPGGHISNCPAELYFGGEVELPF*VT E*ILYISKAKIFIFMHLEYFK*FSDIKKLYEL* VILPKVKGLVVRNCT*D*FIIDAY*NKKRKGWK LQTGSLACFLSVIHCK*HIATTIMLMTNTVTRL* FFLNKGKAVLSWF*TYDGILMSLF*WNQSKYAL *RIYLLYIAAVSLC*SFNTKVT*HNHTIEGNTG YHIGL*YGSWWVLFYPLNFVPMSFVGMGILVLLA LCVSSSPKPPFGENIPLTVAAS*PRITIRELIS FLLLNVGLTIKCKLYIFVLMKIYNLL*QK*MFM VEAF