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Sequences

Sequence NM_001001576

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGCATTCAAAATCAATGAACTGAA
AGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTC
CTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAA
TGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTC
CACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGA
AGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCAC
AGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCC
AACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCA
AAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCC
AATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATG
GACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTG
AGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAG
CTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAG
TTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGT
TCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKHSVQSETCGHQATLRAFKINELK
AEVANHLAVLEKRVE*PTAPVSTVFWITTRS*L
LDAMFPLTPSTCSLQRPSRPCGSRPLTSGFGSP
MRC*AAWSTCTTTSGWSGTSASTLSPSGGGCSAS
TTTTETTPSTTSGTASAWPR*CTAWSGSAVSRR
SSHKRIS*S**QRPSATIWTIPATTTRTRSMPA
QSWRSATMTSHRWRTTTAPWPSRSSPSLSATSSP
TSHLMGSSRSDRE*SH*SWPLTWQDMQKLWILS
KRKWRILTTATRST*PC*R*F**NAVISLTRSV
QWKSQSLGWTVY*RNILCRATVRSQKAFLWHRSW
TETK*PRPQPRLGSSSLS*SQCLKQ*PSSSPWL
RRSCCSHFGNPEIATRS*SG*MTP*KSYRRRLT
A*RLGPPRSPEREAEM*KTVKETVPEESGGRGCS
SGRAGRAARDPCAGKSCPGHLAPQDHVF*EPFC
SLIQKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSTPYKVRPVAIKQLSEHSKSMN*
KLKLQITWLS*RNAWNDQLPL*VQFFG*PQEVDS
STRCSHLPQVPALSRDHRGPAEADL*RLALGAQ
*DAELPGAHVPRPRAGQGLQHQPCHPQEVAVLR
PRQLQKQPLPQLPALLLRGPDDVQHGLALQSPGE
VLTNGYPDPNDSGHLPRSGPSRLQQHVPDQCPH
RAGGPLQ*HLTAGEPPLRRGLPDPRRA*VQHLL
QHPT*WVQADPTGNDHINLGH*HGKTCRNYGFFQ
RENGEF*LQQRGAHDPAEDDFDKML*YL*RGPS
NGSRRALGGLFIRGIFYAERP*EVRRPSCGTVH
GPRQSDQGHSPDWVHQVCPDPNV*NSDQALPHG*
GDHAAATLGIPRSLRGAEADR*RHERVTEED*Q
LDVWGHREVQREKQRCEKQ*RRLCLRKAGGVAA
VLDGLAELRGILVQGRAALGTWHHKTMFSKNHFV
H*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEALRTK*DLWPSSNSPSIQNQ*TE
S*SCKSLGCPRETRGMTNCPCKYSFLDNHKKLT
PRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPN
EMLSCLEHMYHDLGLVRDFSINPVTLRRWLFCV
HDNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQE
KFSQTDILILMTAAICHDLDHPGYNNTYQINART
ELAVRYNDISPLENHHCAVAFQILAEPECNIFS
NIPPDGFKQIRQGMITLILATDMARHAEIMDSF
KEKMENFDYSNEEHMTLLKMILIKCCDISNEVRP
MEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFM
DRDKVTKATAQIGFIKFVLIPMFETVTKLFPMV
EEIMLQPLWESRDRYEELKRIDDAMKELQKKTDS
LTSGATEKSRERSRDVKNSEGDCA*GKRGAWLQ
FWTGWPSCAGSLCREELPWAPGTTRPCFLRTIL
FTDTKKKKGIHDAVQNFIFKLSFK*YILIRK

Sequence NM_001001580

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGG
AATGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCA
GAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGC
TGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTG
CTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATC
TGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACC
ACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGAT
CACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATG
ACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAAT
ATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCAT
CAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTAC
GAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAG
ATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGA
AGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAG
AATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKSIQNQ*TES*SCKSLGCPRETRG
MTNCPCKYSFLDNHKKLTPRRDVPTYPKYLLSP
ETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLG
LVRDFSINPVTLRRWLFCVHDNYRNNPFHNFRHC
FCVAQMMYSMVWLCSLQEKFSQTDILILMTAAI
CHDLDHPGYNNTYQINARTELAVRYNDISPLEN
HHCAVAFQILAEPECNIFSNIPPDGFKQIRQGMI
TLILATDMARHAEIMDSFKEKMENFDYSNEEHM
TLLKMILIKCCDISNEVRPMEVAEPWVDCLLEE
YFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFI
KFVLIPMFETVTKLFPMVEEIMLQPLWESRDRY
EELKRIDDAMKELQKKTDSLTSGATEKSRERSR
DVKNSEGDCA*GKRGAWLQFWTGWPSCAGSLCRE
ELPWAPGTTRPCFLRTILFTDTKKKKGIHDAVQ
NFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRAFKINELKAEVANHLAVLEKRV
E*PTAPVSTVFWITTRS*LLDAMFPLTPSTCSLQ
RPSRPCGSRPLTSGFGSPMRC*AAWSTCTTTSG
WSGTSASTLSPSGGGCSASTTTTETTPSTTSGT
ASAWPR*CTAWSGSAVSRRSSHKRIS*S**QRPS
ATIWTIPATTTRTRSMPAQSWRSATMTSHRWRT
TTAPWPSRSSPSLSATSSPTSHLMGSSRSDRE*
SH*SWPLTWQDMQKLWILSKRKWRILTTATRST*
PC*R*F**NAVISLTRSVQWKSQSLGWTVY*RN
ILCRATVRSQKAFLWHRSWTETK*PRPQPRLGS
SSLS*SQCLKQ*PSSSPWLRRSCCSHFGNPEIAT
RS*SG*MTP*KSYRRRLTA*RLGPPRSPEREAE
M*KTVKETVPEESGGRGCSSGRAGRAARDPCAG
KSCPGHLAPQDHVF*EPFCSLIQKKKKEFMMLYR
ILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEEHSKSMN*KLKLQITWLS*RNAW
NDQLPL*VQFFG*PQEVDSSTRCSHLPQVPALS
RDHRGPAEADL*RLALGAQ*DAELPGAHVPRPRA
GQGLQHQPCHPQEVAVLRPRQLQKQPLPQLPAL
LLRGPDDVQHGLALQSPGEVLTNGYPDPNDSGH
LPRSGPSRLQQHVPDQCPHRAGGPLQ*HLTAGEP
PLRRGLPDPRRA*VQHLLQHPT*WVQADPTGND
HINLGH*HGKTCRNYGFFQRENGEF*LQQRGAH
DPAEDDFDKML*YL*RGPSNGSRRALGGLFIRGI
FYAERP*EVRRPSCGTVHGPRQSDQGHSPDWVH
QVCPDPNV*NSDQALPHG*GDHAAATLGIPRSL
RGAEADR*RHERVTEED*QLDVWGHREVQREKQR
CEKQ*RRLCLRKAGGVAAVLDGLAELRGILVQG
RAALGTWHHKTMFSKNHFVH*YKKKKRNS*CCT
EFYF*TVF*IIYSYTE

Sequence NM_001001575

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGG
AATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCC
CTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCC
CTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCA
GCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCA
GATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGAC
CATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGG
CCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGC
CACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATG
ATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCG
ACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGAT
CCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGG
ATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTG
AAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGC
ACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAA
CTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKSIQNQ*TES*SCKSLGCPRETRG
IGRTKSGGD*EMQE*H*EDEGGAGGQKQQDQLP
L*VQFFG*PQEVDSSTRCSHLPQVPALSRDHRG
PAEADL*RLALGAQ*DAELPGAHVPRPRAGQGLQ
HQPCHPQEVAVLRPRQLQKQPLPQLPALLLRGP
DDVQHGLALQSPGEVLTNGYPDPNDSGHLPRSG
PSRLQQHVPDQCPHRAGGPLQ*HLTAGEPPLRRG
LPDPRRA*VQHLLQHPT*WVQADPTGNDHINLG
H*HGKTCRNYGFFQRENGEF*LQQRGAHDPAED
DFDKML*YL*RGPSNGSRRALGGLFIRGIFYAER
P*EVRRPSCGTVHGPRQSDQGHSPDWVHQVCPD
PNV*NSDQALPHG*GDHAAATLGIPRSLRGAEA
DR*RHERVTEED*QLDVWGHREVQREKQRCEKQ*
RRLCLRKAGGVAAVLDGLAELRGILVQGRAALG
TWHHKTMFSKNHFVH*YKKKKRNS*CCTEFYF*
TVF*IIYSYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRAFKINELKAEVANHLAVLEKRV
ELEGLKVVEIEKCKSDIKKMREELAARSSRTNCP
CKYSFLDNHKKLTPRRDVPTYPKYLLSPETIEA
LRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDF
SINPVTLRRWLFCVHDNYRNNPFHNFRHCFCVAQ
MMYSMVWLCSLQEKFSQTDILILMTAAICHDLD
HPGYNNTYQINARTELAVRYNDISPLENHHCAV
AFQILAEPECNIFSNIPPDGFKQIRQGMITLILA
TDMARHAEIMDSFKEKMENFDYSNEEHMTLLKM
ILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQS
DREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLI
PMFETVTKLFPMVEEIMLQPLWESRDRYEELKR
IDDAMKELQKKTDSLTSGATEKSRERSRDVKNS
EGDCA*GKRGAWLQFWTGWPSCAGSLCREELPWA
PGTTRPCFLRTILFTDTKKKKGIHDAVQNFIFK
LSFK*YILIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEEHSKSMN*KLKLQITWLS*RNAW
NWKD*KWWRLRNARVTLRR*GRSWRPEAAGPTA
PVSTVFWITTRS*LLDAMFPLTPSTCSLQRPSRP
CGSRPLTSGFGSPMRC*AAWSTCTTTSGWSGTS
ASTLSPSGGGCSASTTTTETTPSTTSGTASAWP
R*CTAWSGSAVSRRSSHKRIS*S**QRPSATIWT
IPATTTRTRSMPAQSWRSATMTSHRWRTTTAPW
PSRSSPSLSATSSPTSHLMGSSRSDRE*SH*SW
PLTWQDMQKLWILSKRKWRILTTATRST*PC*R*
F**NAVISLTRSVQWKSQSLGWTVY*RNILCRA
TVRSQKAFLWHRSWTETK*PRPQPRLGSSSLS*
SQCLKQ*PSSSPWLRRSCCSHFGNPEIATRS*SG
*MTP*KSYRRRLTA*RLGPPRSPEREAEM*KTV
KETVPEESGGRGCSSGRAGRAARDPCAGKSCPG
HLAPQDHVF*EPFCSLIQKKKKEFMMLYRILFLN
CLLNNIFLYG

Sequence NM_001001579

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCC
TAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAG
CAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCA
GAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGC
TGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTG
CTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATC
TGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACC
ACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGAT
CACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATG
ACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAAT
ATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCAT
CAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTAC
GAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAG
ATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGA
AGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAG
AATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RTPYKVRPVAIKQLSEHSKSMN*KLKLQITWLS
*RNAWNWKD*KWWRLRNARVTLRR*GRSWRPEA
AGPTAPVSTVFWITTRS*LLDAMFPLTPSTCSLQ
RPSRPCGSRPLTSGFGSPMRC*AAWSTCTTTSG
WSGTSASTLSPSGGGCSASTTTTETTPSTTSGT
ASAWPR*CTAWSGSAVSRRSSHKRIS*S**QRPS
ATIWTIPATTTRTRSMPAQSWRSATMTSHRWRT
TTAPWPSRSSPSLSATSSPTSHLMGSSRSDRE*
SH*SWPLTWQDMQKLWILSKRKWRILTTATRST*
PC*R*F**NAVISLTRSVQWKSQSLGWTVY*RN
ILCRATVRSQKAFLWHRSWTETK*PRPQPRLGS
SSLS*SQCLKQ*PSSSPWLRRSCCSHFGNPEIAT
RS*SG*MTP*KSYRRRLTA*RLGPPRSPEREAE
M*KTVKETVPEESGGRGCSSGRAGRAARDPCAG
KSCPGHLAPQDHVF*EPFCSLIQKKKKEFMMLYR
ILFLNCLLNNIFLYG

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LALRTK*DLWPSSNSPSIQNQ*TES*SCKSLGCP
RETRGIGRTKSGGD*EMQE*H*EDEGGAGGQKQ
QDQLPL*VQFFG*PQEVDSSTRCSHLPQVPALS
RDHRGPAEADL*RLALGAQ*DAELPGAHVPRPRA
GQGLQHQPCHPQEVAVLRPRQLQKQPLPQLPAL
LLRGPDDVQHGLALQSPGEVLTNGYPDPNDSGH
LPRSGPSRLQQHVPDQCPHRAGGPLQ*HLTAGEP
PLRRGLPDPRRA*VQHLLQHPT*WVQADPTGND
HINLGH*HGKTCRNYGFFQRENGEF*LQQRGAH
DPAEDDFDKML*YL*RGPSNGSRRALGGLFIRGI
FYAERP*EVRRPSCGTVHGPRQSDQGHSPDWVH
QVCPDPNV*NSDQALPHG*GDHAAATLGIPRSL
RGAEADR*RHERVTEED*QLDVWGHREVQREKQR
CEKQ*RRLCLRKAGGVAAVLDGLAELRGILVQG
RAALGTWHHKTMFSKNHFVH*YKKKKRNS*CCT
EFYF*TVF*IIYSYTE

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SHSVQSETCGHQATLRAFKINELKAEVANHLAV
LEKRVELEGLKVVEIEKCKSDIKKMREELAARSS
RTNCPCKYSFLDNHKKLTPRRDVPTYPKYLLSP
ETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLG
LVRDFSINPVTLRRWLFCVHDNYRNNPFHNFRHC
FCVAQMMYSMVWLCSLQEKFSQTDILILMTAAI
CHDLDHPGYNNTYQINARTELAVRYNDISPLEN
HHCAVAFQILAEPECNIFSNIPPDGFKQIRQGMI
TLILATDMARHAEIMDSFKEKMENFDYSNEEHM
TLLKMILIKCCDISNEVRPMEVAEPWVDCLLEE
YFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFI
KFVLIPMFETVTKLFPMVEEIMLQPLWESRDRY
EELKRIDDAMKELQKKTDSLTSGATEKSRERSR
DVKNSEGDCA*GKRGAWLQFWTGWPSCAGSLCRE
ELPWAPGTTRPCFLRTILFTDTKKKKGIHDAVQ
NFIFKLSFK*YILIRK

Sequence NM_001001574

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCA
AGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTG
AGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAA
GAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTT
TGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGC
TGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAG
TCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATC
AATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCA
ACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTAT
GGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAAC
GAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGG
CACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTT
CCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAG
AAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGG
CGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAG
AACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGG
AAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RTPYKVRPVAIKQLSEREELIQSVLAQVAEQFS
RAFKINELKAEVANHLAVLEKRVELEGLKVVEI
EKCKSDIKKMREELAARSSRTNCPCKYSFLDNHK
KLTPRRDVPTYPKYLLSPETIEALRKPTFDVWL
WEPNEMLSCLEHMYHDLGLVRDFSINPVTLRRW
LFCVHDNYRNNPFHNFRHCFCVAQMMYSMVWLCS
LQEKFSQTDILILMTAAICHDLDHPGYNNTYQI
NARTELAVRYNDISPLENHHCAVAFQILAEPEC
NIFSNIPPDGFKQIRQGMITLILATDMARHAEIM
DSFKEKMENFDYSNEEHMTLLKMILIKCCDISN
EVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPV
APFMDRDKVTKATAQIGFIKFVLIPMFETVTKLF
PMVEEIMLQPLWESRDRYEELKRIDDAMKELQK
KTDSLTSGATEKSRERSRDVKNSEGDCA*GKRG
AWLQFWTGWPSCAGSLCREELPWAPGTTRPCFLR
TILFTDTKKKKGIHDAVQNFIFKLSFK*YILIR
K

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LALRTK*DLWPSSNSPREKN*SRACWRRLQSSSQ
EHSKSMN*KLKLQITWLS*RNAWNWKD*KWWRL
RNARVTLRR*GRSWRPEAAGPTAPVSTVFWITT
RS*LLDAMFPLTPSTCSLQRPSRPCGSRPLTSGF
GSPMRC*AAWSTCTTTSGWSGTSASTLSPSGGG
CSASTTTTETTPSTTSGTASAWPR*CTAWSGSA
VSRRSSHKRIS*S**QRPSATIWTIPATTTRTRS
MPAQSWRSATMTSHRWRTTTAPWPSRSSPSLSA
TSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQKL
WILSKRKWRILTTATRST*PC*R*F**NAVISLT
RSVQWKSQSLGWTVY*RNILCRATVRSQKAFLW
HRSWTETK*PRPQPRLGSSSLS*SQCLKQ*PSS
SPWLRRSCCSHFGNPEIATRS*SG*MTP*KSYRR
RLTA*RLGPPRSPEREAEM*KTVKETVPEESGG
RGCSSGRAGRAARDPCAGKSCPGHLAPQDHVF*
EPFCSLIQKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SHSVQSETCGHQATLRERRINPERAGAGCRAVL
KSIQNQ*TES*SCKSLGCPRETRGIGRTKSGGD*
EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*PQ
EVDSSTRCSHLPQVPALSRDHRGPAEADL*RLA
LGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQEVA
VLRPRQLQKQPLPQLPALLLRGPDDVQHGLALQ
SPGEVLTNGYPDPNDSGHLPRSGPSRLQQHVPD
QCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA*VQ
HLLQHPT*WVQADPTGNDHINLGH*HGKTCRNY
GFFQRENGEF*LQQRGAHDPAEDDFDKML*YL*
RGPSNGSRRALGGLFIRGIFYAERP*EVRRPSCG
TVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQAL
PHG*GDHAAATLGIPRSLRGAEADR*RHERVTE
ED*QLDVWGHREVQREKQRCEKQ*RRLCLRKAGG
VAAVLDGLAELRGILVQGRAALGTWHHKTMFSK
NHFVH*YKKKKRNS*CCTEFYF*TVF*IIYSYT
E

Sequence NM_002606

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGGGTTGTGG
GCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGCGCATCCC
TCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAAT
CACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGG
CGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTA
CCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTAC
CACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACA
ACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAAT
GACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCA
CCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCC
GACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAA
CGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGT
TTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCC
AGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATC
CCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGA
GAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGAT
CCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCA
TGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSAGVEDKRTTSRGQSAERPLRDRRVV
GLEQPRREGAFESGQVEPRPREPQGCYQEGQRIP
PEREELIQSVLAQVAEQFSRAFKINELKAEVAN
HLAVLEKRVELEGLKVVEIEKCKSDIKKMREEL
AARSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKY
LLSPETIEALRKPTFDVWLWEPNEMLSCLEHMY
HDLGLVRDFSINPVTLRRWLFCVHDNYRNNPFH
NFRHCFCVAQMMYSMVWLCSLQEKFSQTDILILM
TAAICHDLDHPGYNNTYQINARTELAVRYNDIS
PLENHHCAVAFQILAEPECNIFSNIPPDGFKQI
RQGMITLILATDMARHAEIMDSFKEKMENFDYSN
EEHMTLLKMILIKCCDISNEVRPMEVAEPWVDC
LLEEYFMQSDREKSEGLPVAPFMDRDKVTKATA
QIGFIKFVLIPMFETVTKLFPMVEEIMLQPLWES
RDRYEELKRIDDAMKELQKKTDSLTSGATEKSR
ERSRDVKNSEGDCA*GKRGAWLQFWTGWPSCAG
SLCREELPWAPGTTRPCFLRTILFTDTKKKKGIH
DAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPLVSRTREPQAVASLLRDH*GTDGLW
AWSSPGGKEHLKVDR*SPGPESPRAATRKASAS
LQREKN*SRACWRRLQSSSQEHSKSMN*KLKLQI
TWLS*RNAWNWKD*KWWRLRNARVTLRR*GRSW
RPEAAGPTAPVSTVFWITTRS*LLDAMFPLTPS
TCSLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCT
TTSGWSGTSASTLSPSGGGCSASTTTTETTPST
TSGTASAWPR*CTAWSGSAVSRRSSHKRIS*S*
*QRPSATIWTIPATTTRTRSMPAQSWRSATMTSH
RWRTTTAPWPSRSSPSLSATSSPTSHLMGSSRS
DRE*SH*SWPLTWQDMQKLWILSKRKWRILTTA
TRST*PC*R*F**NAVISLTRSVQWKSQSLGWTV
Y*RNILCRATVRSQKAFLWHRSWTETK*PRPQP
RLGSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGN
PEIATRS*SG*MTP*KSYRRRLTA*RLGPPRSPE
REAEM*KTVKETVPEESGGRGCSSGRAGRAARD
PCAGKSCPGHLAPQDHVF*EPFCSLIQKKKKEF
MMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRWCRGQENHKPWPVC*ETTEGQTGCG
PGAAPEGRSI*KWTGRAQAQRAPGLLPGRPAHP
SRERRINPERAGAGCRAVLKSIQNQ*TES*SCK
SLGCPRETRGIGRTKSGGD*EMQE*H*EDEGGAG
GQKQQDQLPL*VQFFG*PQEVDSSTRCSHLPQV
PALSRDHRGPAEADL*RLALGAQ*DAELPGAHV
PRPRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQ
LPALLLRGPDDVQHGLALQSPGEVLTNGYPDPN
DSGHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HL
TAGEPPLRRGLPDPRRA*VQHLLQHPT*WVQADP
TGNDHINLGH*HGKTCRNYGFFQRENGEF*LQQ
RGAHDPAEDDFDKML*YL*RGPSNGSRRALGGL
FIRGIFYAERP*EVRRPSCGTVHGPRQSDQGHSP
DWVHQVCPDPNV*NSDQALPHG*GDHAAATLGI
PRSLRGAEADR*RHERVTEED*QLDVWGHREVQ
REKQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGI
LVQGRAALGTWHHKTMFSKNHFVH*YKKKKRNS
*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001567

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAAC
TGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAA
GAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGAT
GTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGA
GCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTA
CAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAA
ACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGG
TCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACC
TGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATG
GAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCG
CAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAA
AGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATG
CTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTG
GGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGG
CTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACA
AAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSEREELIQSVLAQVAEQFSRAFKINE
LKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIK
KMREELAARSSRTNCPCKYSFLDNHKKLTPRRD
VPTYPKYLLSPETIEALRKPTFDVWLWEPNEML
SCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDNY
RNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFSQ
TDILILMTAAICHDLDHPGYNNTYQINARTELA
VRYNDISPLENHHCAVAFQILAEPECNIFSNIPP
DGFKQIRQGMITLILATDMARHAEIMDSFKEKM
ENFDYSNEEHMTLLKMILIKCCDISNEVRPMEV
AEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDK
VTKATAQIGFIKFVLIPMFETVTKLFPMVEEIM
LQPLWESRDRYEELKRIDDAMKELQKKTDSLTS
GATEKSRERSRDVKNSEGDCA*GKRGAWLQFWTG
WPSCAGSLCREELPWAPGTTRPCFLRTILFTDT
KKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPREKN*SRACWRRLQSSSQEHSKSMN
*KLKLQITWLS*RNAWNWKD*KWWRLRNARVTL
RR*GRSWRPEAAGPTAPVSTVFWITTRS*LLDAM
FPLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC*
AAWSTCTTTSGWSGTSASTLSPSGGGCSASTTT
TETTPSTTSGTASAWPR*CTAWSGSAVSRRSSHK
RIS*S**QRPSATIWTIPATTTRTRSMPAQSWR
SATMTSHRWRTTTAPWPSRSSPSLSATSSPTSH
LMGSSRSDRE*SH*SWPLTWQDMQKLWILSKRKW
RILTTATRST*PC*R*F**NAVISLTRSVQWKS
QSLGWTVY*RNILCRATVRSQKAFLWHRSWTET
K*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRSC
CSHFGNPEIATRS*SG*MTP*KSYRRRLTA*RL
GPPRSPEREAEM*KTVKETVPEESGGRGCSSGR
AGRAARDPCAGKSCPGHLAPQDHVF*EPFCSLIQ
KKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRERRINPERAGAGCRAVLKSIQNQ*T
ES*SCKSLGCPRETRGIGRTKSGGD*EMQE*H*
EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTR
CSHLPQVPALSRDHRGPAEADL*RLALGAQ*DAE
LPGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQL
QKQPLPQLPALLLRGPDDVQHGLALQSPGEVLT
NGYPDPNDSGHLPRSGPSRLQQHVPDQCPHRAGG
PLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHPT
*WVQADPTGNDHINLGH*HGKTCRNYGFFQREN
GEF*LQQRGAHDPAEDDFDKML*YL*RGPSNGSR
RALGGLFIRGIFYAERP*EVRRPSCGTVHGPRQ
SDQGHSPDWVHQVCPDPNV*NSDQALPHG*GDH
AAATLGIPRSLRGAEADR*RHERVTEED*QLDVW
GHREVQREKQRCEKQ*RRLCLRKAGGVAAVLDG
LAELRGILVQGRAALGTWHHKTMFSKNHFVH*Y
KKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001583

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCA
CTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCT
ACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGA
ACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATT
AAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCG
ATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCT
GAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAAC
TACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCAC
AAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGC
GGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCA
CCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAA
TGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGT
CGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGAC
AAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCA
TGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTC
TGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACG
GGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATA
CAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RTPYKVRPVAIKQLSAGVEDKRTTSRGQSAERP
LRDRRVVGLEQPRREGAFESGQVEPRPREPQGC
YQEGQRIPPEREELIQSVLAQVAEQFSRAFKINE
LKAEVANHLAVLEKRVELEGLKVVEIEKCKSDI
KKMREELAARSSRTNCPCKYSFLDNHKKLTPRR
DVPTYPKYLLSPETIEALRKPTFDVWLWEPNEML
SCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDN
YRNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFS
QTDILILMTAAICHDLDHPGYNNTYQINARTELA
VRYNDISPLENHHCAVAFQILAEPECNIFSNIP
PDGFKQIRQGMITLILATDMARHAEIMDSFKEK
MENFDYSNEEHMTLLKMILIKCCDISNEVRPMEV
AEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRD
KVTKATAQIGFIKFVLIPMFETVTKLFPMVEEI
MLQPLWESRDRYEELKRIDDAMKELQKKTDSLTS
GATEKSRERSRDVKNSEGDCA*GKRGAWLQFWT
GWPSCAGSLCREELPWAPGTTRPCFLRTILFTD
TKKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LALRTK*DLWPSSNSPLVSRTREPQAVASLLRDH
*GTDGLWAWSSPGGKEHLKVDR*SPGPESPRAA
TRKASASLQREKN*SRACWRRLQSSSQEHSKSM
N*KLKLQITWLS*RNAWNWKD*KWWRLRNARVTL
RR*GRSWRPEAAGPTAPVSTVFWITTRS*LLDA
MFPLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC
*AAWSTCTTTSGWSGTSASTLSPSGGGCSASTTT
TETTPSTTSGTASAWPR*CTAWSGSAVSRRSSH
KRIS*S**QRPSATIWTIPATTTRTRSMPAQSW
RSATMTSHRWRTTTAPWPSRSSPSLSATSSPTSH
LMGSSRSDRE*SH*SWPLTWQDMQKLWILSKRK
WRILTTATRST*PC*R*F**NAVISLTRSVQWK
SQSLGWTVY*RNILCRATVRSQKAFLWHRSWTET
K*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRS
CCSHFGNPEIATRS*SG*MTP*KSYRRRLTA*R
LGPPRSPEREAEM*KTVKETVPEESGGRGCSSGR
AGRAARDPCAGKSCPGHLAPQDHVF*EPFCSLI
QKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SHSVQSETCGHQATLRWCRGQENHKPWPVC*ET
TEGQTGCGPGAAPEGRSI*KWTGRAQAQRAPGLL
PGRPAHPSRERRINPERAGAGCRAVLKSIQNQ*
TES*SCKSLGCPRETRGIGRTKSGGD*EMQE*H
*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTR
CSHLPQVPALSRDHRGPAEADL*RLALGAQ*DA
ELPGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQ
LQKQPLPQLPALLLRGPDDVQHGLALQSPGEVLT
NGYPDPNDSGHLPRSGPSRLQQHVPDQCPHRAG
GPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHP
T*WVQADPTGNDHINLGH*HGKTCRNYGFFQREN
GEF*LQQRGAHDPAEDDFDKML*YL*RGPSNGS
RRALGGLFIRGIFYAERP*EVRRPSCGTVHGPR
QSDQGHSPDWVHQVCPDPNV*NSDQALPHG*GDH
AAATLGIPRSLRGAEADR*RHERVTEED*QLDV
WGHREVQREKQRCEKQ*RRLCLRKAGGVAAVLD
GLAELRGILVQGRAALGTWHHKTMFSKNHFVH*Y
KKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001573

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGG
GTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGC
GCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGT
TGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAG
GAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACC
CCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCA
CATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCC
TTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGA
TCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGA
CATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAG
CAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACT
ACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGT
GGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCC
ACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTT
GGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAA
GTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGC
GCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAG
GAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKLVSRTREPQAVASLLRDH*GTDG
LWAWSSPGGKEHLKVDR*SPGPESPRAATRKAS
ASLQREKN*SRACWRRLQSSSQEHSKSMN*KLK
LQITWLS*RNAWNWKD*KWWRLRNARVTLRR*GR
SWRPEAAGPTAPVSTVFWITTRS*LLDAMFPLT
PSTCSLQRPSRPCGSRPLTSGFGSPMRC*AAWS
TCTTTSGWSGTSASTLSPSGGGCSASTTTTETTP
STTSGTASAWPR*CTAWSGSAVSRRSSHKRIS*
S**QRPSATIWTIPATTTRTRSMPAQSWRSATM
TSHRWRTTTAPWPSRSSPSLSATSSPTSHLMGSS
RSDRE*SH*SWPLTWQDMQKLWILSKRKWRILT
TATRST*PC*R*F**NAVISLTRSVQWKSQSLG
WTVY*RNILCRATVRSQKAFLWHRSWTETK*PRP
QPRLGSSSLS*SQCLKQ*PSSSPWLRRSCCSHF
GNPEIATRS*SG*MTP*KSYRRRLTA*RLGPPR
SPEREAEM*KTVKETVPEESGGRGCSSGRAGRAA
RDPCAGKSCPGHLAPQDHVF*EPFCSLIQKKKK
EFMMLYRILFLNCLLNNIFLYG

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSWCRGQENHKPWPVC*ETTEGQT
GCGPGAAPEGRSI*KWTGRAQAQRAPGLLPGRPA
HPSRERRINPERAGAGCRAVLKSIQNQ*TES*S
CKSLGCPRETRGIGRTKSGGD*EMQE*H*EDEG
GAGGQKQQDQLPL*VQFFG*PQEVDSSTRCSHLP
QVPALSRDHRGPAEADL*RLALGAQ*DAELPGA
HVPRPRAGQGLQHQPCHPQEVAVLRPRQLQKQP
LPQLPALLLRGPDDVQHGLALQSPGEVLTNGYPD
PNDSGHLPRSGPSRLQQHVPDQCPHRAGGPLQ*
HLTAGEPPLRRGLPDPRRA*VQHLLQHPT*WVQ
ADPTGNDHINLGH*HGKTCRNYGFFQRENGEF*L
QQRGAHDPAEDDFDKML*YL*RGPSNGSRRALG
GLFIRGIFYAERP*EVRRPSCGTVHGPRQSDQG
HSPDWVHQVCPDPNV*NSDQALPHG*GDHAAATL
GIPRSLRGAEADR*RHERVTEED*QLDVWGHRE
VQREKQRCEKQ*RRLCLRKAGGVAAVLDGLAEL
RGILVQGRAALGTWHHKTMFSKNHFVH*YKKKKR
NS*CCTEFYF*TVF*IIYSYTE

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEAGVEDKRTTSRGQSAERPLRDRR
VVGLEQPRREGAFESGQVEPRPREPQGCYQEGQ
RIPPEREELIQSVLAQVAEQFSRAFKINELKAEV
ANHLAVLEKRVELEGLKVVEIEKCKSDIKKMRE
ELAARSSRTNCPCKYSFLDNHKKLTPRRDVPTY
PKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEH
MYHDLGLVRDFSINPVTLRRWLFCVHDNYRNNP
FHNFRHCFCVAQMMYSMVWLCSLQEKFSQTDIL
ILMTAAICHDLDHPGYNNTYQINARTELAVRYND
ISPLENHHCAVAFQILAEPECNIFSNIPPDGFK
QIRQGMITLILATDMARHAEIMDSFKEKMENFD
YSNEEHMTLLKMILIKCCDISNEVRPMEVAEPWV
DCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKA
TAQIGFIKFVLIPMFETVTKLFPMVEEIMLQPL
WESRDRYEELKRIDDAMKELQKKTDSLTSGATEK
SRERSRDVKNSEGDCA*GKRGAWLQFWTGWPSC
AGSLCREELPWAPGTTRPCFLRTILFTDTKKKK
GIHDAVQNFIFKLSFK*YILIRK

Sequence NM_001001581

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCA
CAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGA
GCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAG
CAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGG
TGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGA
TAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGAC
GTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCA
GGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTG
GCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACG
TACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGC
CTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGC
AGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGAT
ATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCC
TTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGAC
CAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAG
TTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAA
AGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATG
TTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATT
CTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKHSVQSETCGHQATLRWCRGQENH
KPWPVC*ETTEGQTGCGPGAAPEGRSI*KWTGR
AQAQRAPGLLPGRPAHPSRERRINPERAGAGCR
AVLKSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG
*PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*
RLALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGL
ALQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQH
VPDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTC
RNYGFFQRENGEF*LQQRGAHDPAEDDFDKML*
YL*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSD
QALPHG*GDHAAATLGIPRSLRGAEADR*RHER
VTEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTM
FSKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIY
SYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSTPYKVRPVAIKQLSAGVEDKRT
TSRGQSAERPLRDRRVVGLEQPRREGAFESGQVE
PRPREPQGCYQEGQRIPPEREELIQSVLAQVAE
QFSRAFKINELKAEVANHLAVLEKRVELEGLKV
VEIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFD
VWLWEPNEMLSCLEHMYHDLGLVRDFSINPVTL
RRWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNT
YQINARTELAVRYNDISPLENHHCAVAFQILAE
PECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCD
ISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEG
LPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKE
LQKKTDSLTSGATEKSRERSRDVKNSEGDCA*G
KRGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YI
LIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEALRTK*DLWPSSNSPLVSRTREP
QAVASLLRDH*GTDGLWAWSSPGGKEHLKVDR*
SPGPESPRAATRKASASLQREKN*SRACWRRLQS
SSQEHSKSMN*KLKLQITWLS*RNAWNWKD*KW
WRLRNARVTLRR*GRSWRPEAAGPTAPVSTVFW
ITTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPS
GGGCSASTTTTETTPSTTSGTASAWPR*CTAWS
GSAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPS
LSATSSPTSHLMGSSRSDRE*SH*SWPLTWQDM
QKLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKA
FLWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*
PSSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEE
SGGRGCSSGRAGRAARDPCAGKSCPGHLAPQDH
VF*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Sequence NM_001001571

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCA
GTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTG
GAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATA
ACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGT
CTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGG
AGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGC
TCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTA
CCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCT
GAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAG
AAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATAT
CTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTT
CCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCA
AGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTT
ACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAG
CGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTT
TTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCT
TATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRERRINPERAGAGCRA
VLKSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*
PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*R
LALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGLA
LQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQHV
PDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTCR
NYGFFQRENGEF*LQQRGAHDPAEDDFDKML*Y
L*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQ
ALPHG*GDHAAATLGIPRSLRGAEADR*RHERV
TEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTMF
SKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIYS
YTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSEREELIQSVLAQVAEQ
FSRAFKINELKAEVANHLAVLEKRVELEGLKVV
EIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFDV
WLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLR
RWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNTY
QINARTELAVRYNDISPLENHHCAVAFQILAEP
ECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCDI
SNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGL
PVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKEL
QKKTDSLTSGATEKSRERSRDVKNSEGDCA*GK
RGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YIL
IRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPREKN*SRACWRRLQS
SSQEHSKSMN*KLKLQITWLS*RNAWNWKD*KWW
RLRNARVTLRR*GRSWRPEAAGPTAPVSTVFWI
TTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPSG
GGCSASTTTTETTPSTTSGTASAWPR*CTAWSG
SAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPSL
SATSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQ
KLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKAF
LWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*P
SSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEES
GGRGCSSGRAGRAARDPCAGKSCPGHLAPQDHV
F*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Sequence NM_001001585

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGA
GAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAG
GGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGT
GGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGC
CCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGG
CCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTT
CAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCC
CAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGG
ACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGT
GGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTG
GCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGA
TGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAG
CGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTG
ATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGC
GGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAG
TGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGG
GCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTA
AACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRWCRGQENHKPWPVC*
ETTEGQTGCGPGAAPEGRSI*KWTGRAQAQRAP
GLLPGRPAHPSRAFKINELKAEVANHLAVLEKRV
ELEGLKVVEIEKCKSDIKKMREELAARSSRTNC
PCKYSFLDNHKKLTPRRDVPTYPKYLLSPETIE
ALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDF
SINPVTLRRWLFCVHDNYRNNPFHNFRHCFCVA
QMMYSMVWLCSLQEKFSQTDILILMTAAICHDL
DHPGYNNTYQINARTELAVRYNDISPLENHHCAV
AFQILAEPECNIFSNIPPDGFKQIRQGMITLIL
ATDMARHAEIMDSFKEKMENFDYSNEEHMTLLK
MILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQS
DREKSEGLPVAPFMDRDKVTKATAQIGFIKFVL
IPMFETVTKLFPMVEEIMLQPLWESRDRYEELK
RIDDAMKELQKKTDSLTSGATEKSRERSRDVKNS
EGDCA*GKRGAWLQFWTGWPSCAGSLCREELPW
APGTTRPCFLRTILFTDTKKKKGIHDAVQNFIF
KLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSAGVEDKRTTSRGQSAE
RPLRDRRVVGLEQPRREGAFESGQVEPRPREPQ
GCYQEGQRIPPEHSKSMN*KLKLQITWLS*RNA
WNWKD*KWWRLRNARVTLRR*GRSWRPEAAGPTA
PVSTVFWITTRS*LLDAMFPLTPSTCSLQRPSR
PCGSRPLTSGFGSPMRC*AAWSTCTTTSGWSGT
SASTLSPSGGGCSASTTTTETTPSTTSGTASAWP
R*CTAWSGSAVSRRSSHKRIS*S**QRPSATIW
TIPATTTRTRSMPAQSWRSATMTSHRWRTTTAP
WPSRSSPSLSATSSPTSHLMGSSRSDRE*SH*SW
PLTWQDMQKLWILSKRKWRILTTATRST*PC*R
*F**NAVISLTRSVQWKSQSLGWTVY*RNILCR
ATVRSQKAFLWHRSWTETK*PRPQPRLGSSSLS*
SQCLKQ*PSSSPWLRRSCCSHFGNPEIATRS*S
G*MTP*KSYRRRLTA*RLGPPRSPEREAEM*KT
VKETVPEESGGRGCSSGRAGRAARDPCAGKSCPG
HLAPQDHVF*EPFCSLIQKKKKEFMMLYRILFL
NCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPLVSRTREPQAVASLL
RDH*GTDGLWAWSSPGGKEHLKVDR*SPGPESPR
AATRKASASLQSIQNQ*TES*SCKSLGCPRETR
GIGRTKSGGD*EMQE*H*EDEGGAGGQKQQDQL
PL*VQFFG*PQEVDSSTRCSHLPQVPALSRDHRG
PAEADL*RLALGAQ*DAELPGAHVPRPRAGQGL
QHQPCHPQEVAVLRPRQLQKQPLPQLPALLLRG
PDDVQHGLALQSPGEVLTNGYPDPNDSGHLPRSG
PSRLQQHVPDQCPHRAGGPLQ*HLTAGEPPLRR
GLPDPRRA*VQHLLQHPT*WVQADPTGNDHINL
GH*HGKTCRNYGFFQRENGEF*LQQRGAHDPAED
DFDKML*YL*RGPSNGSRRALGGLFIRGIFYAE
RP*EVRRPSCGTVHGPRQSDQGHSPDWVHQVCP
DPNV*NSDQALPHG*GDHAAATLGIPRSLRGAEA
DR*RHERVTEED*QLDVWGHREVQREKQRCEKQ
*RRLCLRKAGGVAAVLDGLAELRGILVQGRAAL
GTWHHKTMFSKNHFVH*YKKKKRNS*CCTEFYF*
TVF*IIYSYTE

Sequence NM_001001572

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATC
AATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTG
ACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCG
ACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAG
ATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACG
ACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTT
CTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAG
CTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACA
TCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGA
GAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATG
GAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACC
GAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGA
GATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTG
ACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCT
GGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCAC
TGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKREKN*SRACWRRLQSSSQEHSKS
MN*KLKLQITWLS*RNAWNWKD*KWWRLRNARV
TLRR*GRSWRPEAAGPTAPVSTVFWITTRS*LL
DAMFPLTPSTCSLQRPSRPCGSRPLTSGFGSPMR
C*AAWSTCTTTSGWSGTSASTLSPSGGGCSAST
TTTETTPSTTSGTASAWPR*CTAWSGSAVSRRS
SHKRIS*S**QRPSATIWTIPATTTRTRSMPAQS
WRSATMTSHRWRTTTAPWPSRSSPSLSATSSPT
SHLMGSSRSDRE*SH*SWPLTWQDMQKLWILSK
RKWRILTTATRST*PC*R*F**NAVISLTRSVQW
KSQSLGWTVY*RNILCRATVRSQKAFLWHRSWT
ETK*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLR
RSCCSHFGNPEIATRS*SG*MTP*KSYRRRLTA*
RLGPPRSPEREAEM*KTVKETVPEESGGRGCSS
GRAGRAARDPCAGKSCPGHLAPQDHVF*EPFCS
LIQKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRERRINPERAGAGCRAVLKSIQN
Q*TES*SCKSLGCPRETRGIGRTKSGGD*EMQE*
H*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSS
TRCSHLPQVPALSRDHRGPAEADL*RLALGAQ*
DAELPGAHVPRPRAGQGLQHQPCHPQEVAVLRPR
QLQKQPLPQLPALLLRGPDDVQHGLALQSPGEV
LTNGYPDPNDSGHLPRSGPSRLQQHVPDQCPHR
AGGPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQH
PT*WVQADPTGNDHINLGH*HGKTCRNYGFFQR
ENGEF*LQQRGAHDPAEDDFDKML*YL*RGPSN
GSRRALGGLFIRGIFYAERP*EVRRPSCGTVHGP
RQSDQGHSPDWVHQVCPDPNV*NSDQALPHG*G
DHAAATLGIPRSLRGAEADR*RHERVTEED*QL
DVWGHREVQREKQRCEKQ*RRLCLRKAGGVAAVL
DGLAELRGILVQGRAALGTWHHKTMFSKNHFVH
*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEEREELIQSVLAQVAEQFSRAFKI
NELKAEVANHLAVLEKRVELEGLKVVEIEKCKS
DIKKMREELAARSSRTNCPCKYSFLDNHKKLTPR
RDVPTYPKYLLSPETIEALRKPTFDVWLWEPNE
MLSCLEHMYHDLGLVRDFSINPVTLRRWLFCVH
DNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQEKF
SQTDILILMTAAICHDLDHPGYNNTYQINARTE
LAVRYNDISPLENHHCAVAFQILAEPECNIFSN
IPPDGFKQIRQGMITLILATDMARHAEIMDSFKE
KMENFDYSNEEHMTLLKMILIKCCDISNEVRPM
EVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMD
RDKVTKATAQIGFIKFVLIPMFETVTKLFPMVEE
IMLQPLWESRDRYEELKRIDDAMKELQKKTDSL
TSGATEKSRERSRDVKNSEGDCA*GKRGAWLQF
WTGWPSCAGSLCREELPWAPGTTRPCFLRTILFT
DTKKKKGIHDAVQNFIFKLSFK*YILIRK

Sequence NM_001001584

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCAAAAGAATGCAGATTTTGCATTA
TCTAAAGAGGCGGTTCCGTGAGCCCACTGTGCTGAGTCACATATATTCCATCGGTAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGC
AGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGT
GGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGAT
AACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACG
TCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAG
GAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGG
CTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGT
ACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCC
TGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCA
GAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATA
TCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCT
TCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACC
AAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGT
TACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAA
GCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGT
TTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTC
TTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKHSVQSETCGHQATLRKRMQILHY
LKRRFREPTVLSHIYSIGREKN*SRACWRRLQS
SSQEHSKSMN*KLKLQITWLS*RNAWNWKD*KW
WRLRNARVTLRR*GRSWRPEAAGPTAPVSTVFWI
TTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPS
GGGCSASTTTTETTPSTTSGTASAWPR*CTAWSG
SAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPS
LSATSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQ
KLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKA
FLWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*P
SSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEE
SGGRGCSSGRAGRAARDPCAGKSCPGHLAPQDHV
F*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSTPYKVRPVAIKQLSAKECRFCI
I*RGGSVSPLC*VTYIPSVERRINPERAGAGCRA
VLKSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG
*PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*R
LALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGL
ALQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQHV
PDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTC
RNYGFFQRENGEF*LQQRGAHDPAEDDFDKML*Y
L*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSD
QALPHG*GDHAAATLGIPRSLRGAEADR*RHERV
TEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTM
FSKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIYS
YTE

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEALRTK*DLWPSSNSPQKNADFAL
SKEAVP*AHCAESHIFHR*REELIQSVLAQVAE
QFSRAFKINELKAEVANHLAVLEKRVELEGLKVV
EIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFD
VWLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLR
RWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNT
YQINARTELAVRYNDISPLENHHCAVAFQILAEP
ECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCD
ISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGL
PVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKE
LQKKTDSLTSGATEKSRERSRDVKNSEGDCA*GK
RGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YI
LIRK

Sequence NM_001001569

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGCATTCAAAATCAATGAACTGAA
AGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAG
ATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTC
CCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTG
CCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGA
AACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGG
ATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCG
CTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGAT
GGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGA
ATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGA
GCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTG
ACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGC
AGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGC
CACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGG
CCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAA
AAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKHSVQSETCGHQATLRAFKINELK
AEVANHLAVLEKRVELEGLKVVEIEKCKSDIKK
MREELAARSSRTNCPCKYSFLDNHKKLTPRRDV
PTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSC
LEHMYHDLGLVRDFSINPVTLRRWLFCVHDNYR
NNPFHNFRHCFCVAQMMYSMVWLCSLQEKFSQT
DILILMTAAICHDLDHPGYNNTYQINARTELAVR
YNDISPLENHHCAVAFQILAEPECNIFSNIPPD
GFKQIRQGMITLILATDMARHAEIMDSFKEKME
NFDYSNEEHMTLLKMILIKCCDISNEVRPMEVAE
PWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKV
TKATAQIGFIKFVLIPMFETVTKLFPMVEEIML
QPLWESRDRYEELKRIDDAMKELQKKTDSLTSGA
TEKSRERSRDVKNSEGDCA*GKRGAWLQFWTGW
PSCAGSLCREELPWAPGTTRPCFLRTILFTDTK
KKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSTPYKVRPVAIKQLSEHSKSMN*
KLKLQITWLS*RNAWNWKD*KWWRLRNARVTLRR
*GRSWRPEAAGPTAPVSTVFWITTRS*LLDAMF
PLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC*A
AWSTCTTTSGWSGTSASTLSPSGGGCSASTTTTE
TTPSTTSGTASAWPR*CTAWSGSAVSRRSSHKR
IS*S**QRPSATIWTIPATTTRTRSMPAQSWRS
ATMTSHRWRTTTAPWPSRSSPSLSATSSPTSHLM
GSSRSDRE*SH*SWPLTWQDMQKLWILSKRKWR
ILTTATRST*PC*R*F**NAVISLTRSVQWKSQ
SLGWTVY*RNILCRATVRSQKAFLWHRSWTETK*
PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRSCC
SHFGNPEIATRS*SG*MTP*KSYRRRLTA*RLG
PPRSPEREAEM*KTVKETVPEESGGRGCSSGRAG
RAARDPCAGKSCPGHLAPQDHVF*EPFCSLIQK
KKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEALRTK*DLWPSSNSPSIQNQ*TE
S*SCKSLGCPRETRGIGRTKSGGD*EMQE*H*E
DEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTRCS
HLPQVPALSRDHRGPAEADL*RLALGAQ*DAEL
PGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQLQ
KQPLPQLPALLLRGPDDVQHGLALQSPGEVLTNG
YPDPNDSGHLPRSGPSRLQQHVPDQCPHRAGGP
LQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHPT*
WVQADPTGNDHINLGH*HGKTCRNYGFFQRENGE
F*LQQRGAHDPAEDDFDKML*YL*RGPSNGSRR
ALGGLFIRGIFYAERP*EVRRPSCGTVHGPRQS
DQGHSPDWVHQVCPDPNV*NSDQALPHG*GDHAA
ATLGIPRSLRGAEADR*RHERVTEED*QLDVWG
HREVQREKQRCEKQ*RRLCLRKAGGVAAVLDGL
AELRGILVQGRAALGTWHHKTMFSKNHFVH*YKK
KKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001570

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGGAATGAGCTCATTCTCTAT
ACATCACTCCGTAACTTGTTGTTTTTACCTAGTAAGGAGTCATGGGCGTCCCACCAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCC
GAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGA
GAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAG
AAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTT
GGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCT
GTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGT
CTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCA
ATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAA
CATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATG
GATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACG
AGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGC
ACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTC
CCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGA
AGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGC
GTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGA
ACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGA
AA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERNELILY
TSLRNLLFLPSKESWASHQHSVQSETCGHQATL
RAFKINELKAEVANHLAVLEKRVELEGLKVVEIE
KCKSDIKKMREELAARSSRTNCPCKYSFLDNHK
KLTPRRDVPTYPKYLLSPETIEALRKPTFDVWL
WEPNEMLSCLEHMYHDLGLVRDFSINPVTLRRWL
FCVHDNYRNNPFHNFRHCFCVAQMMYSMVWLCS
LQEKFSQTDILILMTAAICHDLDHPGYNNTYQI
NARTELAVRYNDISPLENHHCAVAFQILAEPECN
IFSNIPPDGFKQIRQGMITLILATDMARHAEIM
DSFKEKMENFDYSNEEHMTLLKMILIKCCDISN
EVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVA
PFMDRDKVTKATAQIGFIKFVLIPMFETVTKLF
PMVEEIMLQPLWESRDRYEELKRIDDAMKELQK
KTDSLTSGATEKSRERSRDVKNSEGDCA*GKRGA
WLQFWTGWPSCAGSLCREELPWAPGTTRPCFLR
TILFTDTKKKKGIHDAVQNFIFKLSFK*YILIR
K

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNGMSSFSI
HHSVTCCFYLVRSHGRPTSTPYKVRPVAIKQLS
EHSKSMN*KLKLQITWLS*RNAWNWKD*KWWRL
RNARVTLRR*GRSWRPEAAGPTAPVSTVFWITTR
S*LLDAMFPLTPSTCSLQRPSRPCGSRPLTSGF
GSPMRC*AAWSTCTTTSGWSGTSASTLSPSGGG
CSASTTTTETTPSTTSGTASAWPR*CTAWSGSAV
SRRSSHKRIS*S**QRPSATIWTIPATTTRTRS
MPAQSWRSATMTSHRWRTTTAPWPSRSSPSLSA
TSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQKLW
ILSKRKWRILTTATRST*PC*R*F**NAVISLT
RSVQWKSQSLGWTVY*RNILCRATVRSQKAFLW
HRSWTETK*PRPQPRLGSSSLS*SQCLKQ*PSSS
PWLRRSCCSHFGNPEIATRS*SG*MTP*KSYRR
RLTA*RLGPPRSPEREAEM*KTVKETVPEESGG
RGCSSGRAGRAARDPCAGKSCPGHLAPQDHVF*E
PFCSLIQKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTE*AHSL
YITP*LVVFT**GVMGVPPALRTK*DLWPSSNSP
SIQNQ*TES*SCKSLGCPRETRGIGRTKSGGD*
EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*PQ
EVDSSTRCSHLPQVPALSRDHRGPAEADL*RLAL
GAQ*DAELPGAHVPRPRAGQGLQHQPCHPQEVA
VLRPRQLQKQPLPQLPALLLRGPDDVQHGLALQ
SPGEVLTNGYPDPNDSGHLPRSGPSRLQQHVPDQ
CPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA*VQ
HLLQHPT*WVQADPTGNDHINLGH*HGKTCRNY
GFFQRENGEF*LQQRGAHDPAEDDFDKML*YL*R
GPSNGSRRALGGLFIRGIFYAERP*EVRRPSCG
TVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQAL
PHG*GDHAAATLGIPRSLRGAEADR*RHERVTEE
D*QLDVWGHREVQREKQRCEKQ*RRLCLRKAGG
VAAVLDGLAELRGILVQGRAALGTWHHKTMFSK
NHFVH*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001577

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGT
GGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGAT
AACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACG
TCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAG
GAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGG
CTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGT
ACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCC
TGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCA
GAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATA
TCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCT
TCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACC
AAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGT
TACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAA
GCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGT
TTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTC
TTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG
*PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*R
LALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGL
ALQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQHV
PDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTC
RNYGFFQRENGEF*LQQRGAHDPAEDDFDKML*Y
L*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSD
QALPHG*GDHAAATLGIPRSLRGAEADR*RHERV
TEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTM
FSKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIYS
YTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERAFKINELKAEVANHLAVLEKRVELEGLKVV
EIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFD
VWLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLR
RWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNT
YQINARTELAVRYNDISPLENHHCAVAFQILAEP
ECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCD
ISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGL
PVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKE
LQKKTDSLTSGATEKSRERSRDVKNSEGDCA*GK
RGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YI
LIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNEHSKSMN*KLKLQITWLS*RNAWNWKD*KW
WRLRNARVTLRR*GRSWRPEAAGPTAPVSTVFWI
TTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPS
GGGCSASTTTTETTPSTTSGTASAWPR*CTAWSG
SAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPS
LSATSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQ
KLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKA
FLWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*P
SSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEE
SGGRGCSSGRAGRAARDPCAGKSCPGHLAPQDHV
F*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Sequence NM_001001568

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCG
TGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGA
ATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCC
TGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCC
TGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAG
CATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAG
ATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACC
ATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGC
CTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCC
ACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGA
TTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGA
CCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATC
CCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGA
TAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGA
AGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCA
CCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAAC
TGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKHSVQSETCGHQATLRERRINPER
AGAGCRAVLKSIQNQ*TES*SCKSLGCPRETRG
IGRTKSGGD*EMQE*H*EDEGGAGGQKQQDQLP
L*VQFFG*PQEVDSSTRCSHLPQVPALSRDHRGP
AEADL*RLALGAQ*DAELPGAHVPRPRAGQGLQ
HQPCHPQEVAVLRPRQLQKQPLPQLPALLLRGP
DDVQHGLALQSPGEVLTNGYPDPNDSGHLPRSGP
SRLQQHVPDQCPHRAGGPLQ*HLTAGEPPLRRG
LPDPRRA*VQHLLQHPT*WVQADPTGNDHINLG
H*HGKTCRNYGFFQRENGEF*LQQRGAHDPAEDD
FDKML*YL*RGPSNGSRRALGGLFIRGIFYAER
P*EVRRPSCGTVHGPRQSDQGHSPDWVHQVCPD
PNV*NSDQALPHG*GDHAAATLGIPRSLRGAEAD
R*RHERVTEED*QLDVWGHREVQREKQRCEKQ*
RRLCLRKAGGVAAVLDGLAELRGILVQGRAALG
TWHHKTMFSKNHFVH*YKKKKRNS*CCTEFYF*T
VF*IIYSYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRSTPYKVRPVAIKQLSEREELIQS
VLAQVAEQFSRAFKINELKAEVANHLAVLEKRVE
LEGLKVVEIEKCKSDIKKMREELAARSSRTNCP
CKYSFLDNHKKLTPRRDVPTYPKYLLSPETIEA
LRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFS
INPVTLRRWLFCVHDNYRNNPFHNFRHCFCVAQ
MMYSMVWLCSLQEKFSQTDILILMTAAICHDLD
HPGYNNTYQINARTELAVRYNDISPLENHHCAVA
FQILAEPECNIFSNIPPDGFKQIRQGMITLILA
TDMARHAEIMDSFKEKMENFDYSNEEHMTLLKM
ILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSD
REKSEGLPVAPFMDRDKVTKATAQIGFIKFVLI
PMFETVTKLFPMVEEIMLQPLWESRDRYEELKR
IDDAMKELQKKTDSLTSGATEKSRERSRDVKNSE
GDCA*GKRGAWLQFWTGWPSCAGSLCREELPWA
PGTTRPCFLRTILFTDTKKKKGIHDAVQNFIFK
LSFK*YILIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEALRTK*DLWPSSNSPREKN*SRA
CWRRLQSSSQEHSKSMN*KLKLQITWLS*RNAW
NWKD*KWWRLRNARVTLRR*GRSWRPEAAGPTAP
VSTVFWITTRS*LLDAMFPLTPSTCSLQRPSRP
CGSRPLTSGFGSPMRC*AAWSTCTTTSGWSGTS
ASTLSPSGGGCSASTTTTETTPSTTSGTASAWPR
*CTAWSGSAVSRRSSHKRIS*S**QRPSATIWT
IPATTTRTRSMPAQSWRSATMTSHRWRTTTAPW
PSRSSPSLSATSSPTSHLMGSSRSDRE*SH*SWP
LTWQDMQKLWILSKRKWRILTTATRST*PC*R*
F**NAVISLTRSVQWKSQSLGWTVY*RNILCRA
TVRSQKAFLWHRSWTETK*PRPQPRLGSSSLS*S
QCLKQ*PSSSPWLRRSCCSHFGNPEIATRS*SG
*MTP*KSYRRRLTA*RLGPPRSPEREAEM*KTV
KETVPEESGGRGCSSGRAGRAARDPCAGKSCPGH
LAPQDHVF*EPFCSLIQKKKKEFMMLYRILFLN
CLLNNIFLYG

Sequence NM_001001578

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTG
GCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCA
GAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCT
CTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGAC
CTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCC
GGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGC
GGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTG
GAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGG
GAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGA
GCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTA
GAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTG
GGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGA
TCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGA
AGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGT
GCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGC
TGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRAFKINELKAEVANHL
AVLEKRVELEGLKVVEIEKCKSDIKKMREELAA
RSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKYLL
SPETIEALRKPTFDVWLWEPNEMLSCLEHMYHD
LGLVRDFSINPVTLRRWLFCVHDNYRNNPFHNF
RHCFCVAQMMYSMVWLCSLQEKFSQTDILILMTA
AICHDLDHPGYNNTYQINARTELAVRYNDISPL
ENHHCAVAFQILAEPECNIFSNIPPDGFKQIRQ
GMITLILATDMARHAEIMDSFKEKMENFDYSNEE
HMTLLKMILIKCCDISNEVRPMEVAEPWVDCLL
EEYFMQSDREKSEGLPVAPFMDRDKVTKATAQI
GFIKFVLIPMFETVTKLFPMVEEIMLQPLWESRD
RYEELKRIDDAMKELQKKTDSLTSGATEKSRER
SRDVKNSEGDCA*GKRGAWLQFWTGWPSCAGSL
CREELPWAPGTTRPCFLRTILFTDTKKKKGIHDA
VQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSEHSKSMN*KLKLQITW
LS*RNAWNWKD*KWWRLRNARVTLRR*GRSWRP
EAAGPTAPVSTVFWITTRS*LLDAMFPLTPSTC
SLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCTTT
SGWSGTSASTLSPSGGGCSASTTTTETTPSTTS
GTASAWPR*CTAWSGSAVSRRSSHKRIS*S**Q
RPSATIWTIPATTTRTRSMPAQSWRSATMTSHRW
RTTTAPWPSRSSPSLSATSSPTSHLMGSSRSDR
E*SH*SWPLTWQDMQKLWILSKRKWRILTTATR
ST*PC*R*F**NAVISLTRSVQWKSQSLGWTVY*
RNILCRATVRSQKAFLWHRSWTETK*PRPQPRL
GSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGNPE
IATRS*SG*MTP*KSYRRRLTA*RLGPPRSPERE
AEM*KTVKETVPEESGGRGCSSGRAGRAARDPC
AGKSCPGHLAPQDHVF*EPFCSLIQKKKKEFMM
LYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPSIQNQ*TES*SCKSL
GCPRETRGIGRTKSGGD*EMQE*H*EDEGGAGGQ
KQQDQLPL*VQFFG*PQEVDSSTRCSHLPQVPA
LSRDHRGPAEADL*RLALGAQ*DAELPGAHVPR
PRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQLP
ALLLRGPDDVQHGLALQSPGEVLTNGYPDPNDS
GHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HLTA
GEPPLRRGLPDPRRA*VQHLLQHPT*WVQADPTG
NDHINLGH*HGKTCRNYGFFQRENGEF*LQQRG
AHDPAEDDFDKML*YL*RGPSNGSRRALGGLFI
RGIFYAERP*EVRRPSCGTVHGPRQSDQGHSPDW
VHQVCPDPNV*NSDQALPHG*GDHAAATLGIPR
SLRGAEADR*RHERVTEED*QLDVWGHREVQRE
KQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGILV
QGRAALGTWHHKTMFSKNHFVH*YKKKKRNS*C
CTEFYF*TVF*IIYSYTE

Sequence NM_001001582

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGA
GAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAG
GGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAA
TCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAG
TGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCT
CGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATG
AGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCA
CGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAG
TTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAG
AGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAA
CATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAA
GAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAA
TGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGA
CCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAG
GAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCT
TGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTT
CTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTC
ACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F5 (forward primer)
  • refseq_PDE9A.R5 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRWCRGQENHKPWPVC*
ETTEGQTGCGPGAAPEGRSI*KWTGRAQAQRAP
GLLPGRPAHPSRERRINPERAGAGCRAVLKSIQN
Q*TES*SCKSLGCPRETRGIGRTKSGGD*EMQE
*H*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDS
STRCSHLPQVPALSRDHRGPAEADL*RLALGAQ*
DAELPGAHVPRPRAGQGLQHQPCHPQEVAVLRP
RQLQKQPLPQLPALLLRGPDDVQHGLALQSPGE
VLTNGYPDPNDSGHLPRSGPSRLQQHVPDQCPHR
AGGPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQ
HPT*WVQADPTGNDHINLGH*HGKTCRNYGFFQ
RENGEF*LQQRGAHDPAEDDFDKML*YL*RGPSN
GSRRALGGLFIRGIFYAERP*EVRRPSCGTVHG
PRQSDQGHSPDWVHQVCPDPNV*NSDQALPHG*
GDHAAATLGIPRSLRGAEADR*RHERVTEED*QL
DVWGHREVQREKQRCEKQ*RRLCLRKAGGVAAV
LDGLAELRGILVQGRAALGTWHHKTMFSKNHFV
H*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSAGVEDKRTTSRGQSAE
RPLRDRRVVGLEQPRREGAFESGQVEPRPREPQ
GCYQEGQRIPPEREELIQSVLAQVAEQFSRAFK
INELKAEVANHLAVLEKRVELEGLKVVEIEKCKS
DIKKMREELAARSSRTNCPCKYSFLDNHKKLTP
RRDVPTYPKYLLSPETIEALRKPTFDVWLWEPN
EMLSCLEHMYHDLGLVRDFSINPVTLRRWLFCVH
DNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQEK
FSQTDILILMTAAICHDLDHPGYNNTYQINART
ELAVRYNDISPLENHHCAVAFQILAEPECNIFSN
IPPDGFKQIRQGMITLILATDMARHAEIMDSFK
EKMENFDYSNEEHMTLLKMILIKCCDISNEVRP
MEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMD
RDKVTKATAQIGFIKFVLIPMFETVTKLFPMVE
EIMLQPLWESRDRYEELKRIDDAMKELQKKTDS
LTSGATEKSRERSRDVKNSEGDCA*GKRGAWLQF
WTGWPSCAGSLCREELPWAPGTTRPCFLRTILF
TDTKKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPLVSRTREPQAVASLL
RDH*GTDGLWAWSSPGGKEHLKVDR*SPGPESPR
AATRKASASLQREKN*SRACWRRLQSSSQEHSK
SMN*KLKLQITWLS*RNAWNWKD*KWWRLRNAR
VTLRR*GRSWRPEAAGPTAPVSTVFWITTRS*LL
DAMFPLTPSTCSLQRPSRPCGSRPLTSGFGSPM
RC*AAWSTCTTTSGWSGTSASTLSPSGGGCSAS
TTTTETTPSTTSGTASAWPR*CTAWSGSAVSRRS
SHKRIS*S**QRPSATIWTIPATTTRTRSMPAQ
SWRSATMTSHRWRTTTAPWPSRSSPSLSATSSP
TSHLMGSSRSDRE*SH*SWPLTWQDMQKLWILSK
RKWRILTTATRST*PC*R*F**NAVISLTRSVQ
WKSQSLGWTVY*RNILCRATVRSQKAFLWHRSW
TETK*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLR
RSCCSHFGNPEIATRS*SG*MTP*KSYRRRLTA
*RLGPPRSPEREAEM*KTVKETVPEESGGRGCS
SGRAGRAARDPCAGKSCPGHLAPQDHVF*EPFCS
LIQKKKKEFMMLYRILFLNCLLNNIFLYG

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