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Sequences

Sequence NM_001001583

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCA
CTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCT
ACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGA
ACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATT
AAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCG
ATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCT
GAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAAC
TACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCAC
AAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGC
GGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCA
CCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAA
TGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGT
CGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGAC
AAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCA
TGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTC
TGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACG
GGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATA
CAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F8 (forward primer)
  • refseq_PDE9A.R8 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RTPYKVRPVAIKQLSAGVEDKRTTSRGQSAERP
LRDRRVVGLEQPRREGAFESGQVEPRPREPQGC
YQEGQRIPPEREELIQSVLAQVAEQFSRAFKINE
LKAEVANHLAVLEKRVELEGLKVVEIEKCKSDI
KKMREELAARSSRTNCPCKYSFLDNHKKLTPRR
DVPTYPKYLLSPETIEALRKPTFDVWLWEPNEML
SCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDN
YRNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFS
QTDILILMTAAICHDLDHPGYNNTYQINARTELA
VRYNDISPLENHHCAVAFQILAEPECNIFSNIP
PDGFKQIRQGMITLILATDMARHAEIMDSFKEK
MENFDYSNEEHMTLLKMILIKCCDISNEVRPMEV
AEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRD
KVTKATAQIGFIKFVLIPMFETVTKLFPMVEEI
MLQPLWESRDRYEELKRIDDAMKELQKKTDSLTS
GATEKSRERSRDVKNSEGDCA*GKRGAWLQFWT
GWPSCAGSLCREELPWAPGTTRPCFLRTILFTD
TKKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LALRTK*DLWPSSNSPLVSRTREPQAVASLLRDH
*GTDGLWAWSSPGGKEHLKVDR*SPGPESPRAA
TRKASASLQREKN*SRACWRRLQSSSQEHSKSM
N*KLKLQITWLS*RNAWNWKD*KWWRLRNARVTL
RR*GRSWRPEAAGPTAPVSTVFWITTRS*LLDA
MFPLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC
*AAWSTCTTTSGWSGTSASTLSPSGGGCSASTTT
TETTPSTTSGTASAWPR*CTAWSGSAVSRRSSH
KRIS*S**QRPSATIWTIPATTTRTRSMPAQSW
RSATMTSHRWRTTTAPWPSRSSPSLSATSSPTSH
LMGSSRSDRE*SH*SWPLTWQDMQKLWILSKRK
WRILTTATRST*PC*R*F**NAVISLTRSVQWK
SQSLGWTVY*RNILCRATVRSQKAFLWHRSWTET
K*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRS
CCSHFGNPEIATRS*SG*MTP*KSYRRRLTA*R
LGPPRSPEREAEM*KTVKETVPEESGGRGCSSGR
AGRAARDPCAGKSCPGHLAPQDHVF*EPFCSLI
QKKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SHSVQSETCGHQATLRWCRGQENHKPWPVC*ET
TEGQTGCGPGAAPEGRSI*KWTGRAQAQRAPGLL
PGRPAHPSRERRINPERAGAGCRAVLKSIQNQ*
TES*SCKSLGCPRETRGIGRTKSGGD*EMQE*H
*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTR
CSHLPQVPALSRDHRGPAEADL*RLALGAQ*DA
ELPGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQ
LQKQPLPQLPALLLRGPDDVQHGLALQSPGEVLT
NGYPDPNDSGHLPRSGPSRLQQHVPDQCPHRAG
GPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHP
T*WVQADPTGNDHINLGH*HGKTCRNYGFFQREN
GEF*LQQRGAHDPAEDDFDKML*YL*RGPSNGS
RRALGGLFIRGIFYAERP*EVRRPSCGTVHGPR
QSDQGHSPDWVHQVCPDPNV*NSDQALPHG*GDH
AAATLGIPRSLRGAEADR*RHERVTEED*QLDV
WGHREVQREKQRCEKQ*RRLCLRKAGGVAAVLD
GLAELRGILVQGRAALGTWHHKTMFSKNHFVH*Y
KKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_002606

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGGGTTGTGG
GCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGCGCATCCC
TCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAAT
CACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGG
CGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTA
CCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTAC
CACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACA
ACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAAT
GACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCA
CCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCC
GACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAA
CGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGT
TTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCC
AGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATC
CCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGA
GAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGAT
CCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCA
TGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F8 (forward primer)
  • refseq_PDE9A.R8 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSAGVEDKRTTSRGQSAERPLRDRRVV
GLEQPRREGAFESGQVEPRPREPQGCYQEGQRIP
PEREELIQSVLAQVAEQFSRAFKINELKAEVAN
HLAVLEKRVELEGLKVVEIEKCKSDIKKMREEL
AARSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKY
LLSPETIEALRKPTFDVWLWEPNEMLSCLEHMY
HDLGLVRDFSINPVTLRRWLFCVHDNYRNNPFH
NFRHCFCVAQMMYSMVWLCSLQEKFSQTDILILM
TAAICHDLDHPGYNNTYQINARTELAVRYNDIS
PLENHHCAVAFQILAEPECNIFSNIPPDGFKQI
RQGMITLILATDMARHAEIMDSFKEKMENFDYSN
EEHMTLLKMILIKCCDISNEVRPMEVAEPWVDC
LLEEYFMQSDREKSEGLPVAPFMDRDKVTKATA
QIGFIKFVLIPMFETVTKLFPMVEEIMLQPLWES
RDRYEELKRIDDAMKELQKKTDSLTSGATEKSR
ERSRDVKNSEGDCA*GKRGAWLQFWTGWPSCAG
SLCREELPWAPGTTRPCFLRTILFTDTKKKKGIH
DAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPLVSRTREPQAVASLLRDH*GTDGLW
AWSSPGGKEHLKVDR*SPGPESPRAATRKASAS
LQREKN*SRACWRRLQSSSQEHSKSMN*KLKLQI
TWLS*RNAWNWKD*KWWRLRNARVTLRR*GRSW
RPEAAGPTAPVSTVFWITTRS*LLDAMFPLTPS
TCSLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCT
TTSGWSGTSASTLSPSGGGCSASTTTTETTPST
TSGTASAWPR*CTAWSGSAVSRRSSHKRIS*S*
*QRPSATIWTIPATTTRTRSMPAQSWRSATMTSH
RWRTTTAPWPSRSSPSLSATSSPTSHLMGSSRS
DRE*SH*SWPLTWQDMQKLWILSKRKWRILTTA
TRST*PC*R*F**NAVISLTRSVQWKSQSLGWTV
Y*RNILCRATVRSQKAFLWHRSWTETK*PRPQP
RLGSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGN
PEIATRS*SG*MTP*KSYRRRLTA*RLGPPRSPE
REAEM*KTVKETVPEESGGRGCSSGRAGRAARD
PCAGKSCPGHLAPQDHVF*EPFCSLIQKKKKEF
MMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRWCRGQENHKPWPVC*ETTEGQTGCG
PGAAPEGRSI*KWTGRAQAQRAPGLLPGRPAHP
SRERRINPERAGAGCRAVLKSIQNQ*TES*SCK
SLGCPRETRGIGRTKSGGD*EMQE*H*EDEGGAG
GQKQQDQLPL*VQFFG*PQEVDSSTRCSHLPQV
PALSRDHRGPAEADL*RLALGAQ*DAELPGAHV
PRPRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQ
LPALLLRGPDDVQHGLALQSPGEVLTNGYPDPN
DSGHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HL
TAGEPPLRRGLPDPRRA*VQHLLQHPT*WVQADP
TGNDHINLGH*HGKTCRNYGFFQRENGEF*LQQ
RGAHDPAEDDFDKML*YL*RGPSNGSRRALGGL
FIRGIFYAERP*EVRRPSCGTVHGPRQSDQGHSP
DWVHQVCPDPNV*NSDQALPHG*GDHAAATLGI
PRSLRGAEADR*RHERVTEED*QLDVWGHREVQ
REKQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGI
LVQGRAALGTWHHKTMFSKNHFVH*YKKKKRNS
*CCTEFYF*TVF*IIYSYTE

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