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Sequences

Sequence NM_002605

CCGCCGCCGCCGCAGCGCCCGCACCGCGATAAAAGGGGCGGCCGCGTTTCCTGACGCGAGATCCGCGCTCGCCGCCGCCCGCCCAGGCGGCGATGACACG GCGCCCGCGGCGGCCCGGAGGCGCCGGGTGGGCCGTTTGCTGACCGGATCGCGGCTACCCGCCAGCGTGTCCGCGGCGCCGCCGCCAGCATGGGCTGTGC CCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGCCCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAG AAGACGGCCGCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGCAAGAAGGTAGCAGTAGCTGATGTGCAGTTTG GCCCCATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGTGAAAAAGCAGG GTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTCCTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAG CTGGATGCAGAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGTGTAGTACGCAGGGTGGATAGAGAAGAGTTGT CCGTAATGCCTTTCATTTCTGCTGGATTTACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTGGAGTTTGGAGA GGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCATTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATA CAGTATGCAAATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGAGTTAGGAGAAGTGCCTATAAATGAAAAAAAGGCTG ACTTGCTCGATACTATAAATTCATGCATCAGGATAGGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGT GAAGATAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGCAATGGCAACAATAAGGCTGAGAAAATATCCGAA TGTGTTCAGTCTGACACTCATACAGATAATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCCTCCCGTGCAACTG AAGTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATCAATGCTGCCCA GGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTAAGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGAT GATCCCCATGCCAATGACCTTGTTGGGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAAAACACTCAAATGG TTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCACGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGA ACTGGAGGCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACG CTAAGATCATGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCTGATGTGCTTCATGCCACTGCCTATT TTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATGAGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAAC CAACTCCTTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTGGCCTTCCAGCTGACC ACTGGAGATGATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTAGCCACAGAAATGA CAAAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAAGTGAT AAACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTGATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGC ATCGAGTGGGCTGCACGCATTTCGGAAGAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTGTTTGACAGAAATA CCTGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCA GCATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCACCTCCTGAATAGTGGGAGACACCACCCAGAGCC CTGAAGCTTTGTTCCTTCGGTCATTTGGAATTCCTGAGGGCAGCCAGAGCTCCTTGGTCCTTTCAGTACTAGGCAGAACAGCCCCCGATCTGCATAGCCT GTGAAAGCCCACGGGGACATCAGTAACCTTCTGCAGCCACCATCCAATGCCATTACTGTCAAGTGAGACTTGGCCACTGTAGCCTGGGCCTGCTGCAGGA GCTCTTCAGAAAGGCACATGAGGACCACGGTTTGCCTCAGTTTCTGGTAAAACACAAGGTCTGGAGTGCCCCTGCAAAGGGTATTGATGGACTTCCTGCC AGTGACAGAGCATGTCTATTGCAAACAATTCTCTCAGTTACGTTCAGCACTTAAGAACGGCTAATGGCAATAGGATCTTTAGCAACTTTTTCACATCATA GAAGGTGCAATCGCTCACTTGGGAACACTACTGAGAGTGACTTCTCTTTTAAAATTGAGTAGCAGATGAAAAATTAAAATTTGAACTTGATTATTAATAT CAATTAAAATGTTTTATTTATTTTATTAAAAGCTCAATATTTTCTATGAATTCAAAAATACTTCAGAGCCAAAGCCAACTTCAAATACCGTGACCAAATT TACATGATTCATATTCATTATGCATTACTTGGTATACAGACTTATTTTCATAATGCAAATTAATAAAATGACACTTTTACTGCACTATAGAAATATTCAT GTATGTTAAACTTTTCTGATTGAGGCTAACTGGAAAAAGCTGGGGTCGTATTCTAAGTGCTAAAGAAGGCTGCTTCTACTGTATAGAACCCAGGGCTCTG AAACAGCTCTAGCCGCCTAATGCACTTCACAGGTAACTCCCCAAGGTAAAACTAGACTCTCTTGTTGGTTCGCAAAGAAAAGTTAGGACTTAACACTTTT TTCTAAAATTTTATAATTCAATTTCCAAAAGTCTACTCTATTTTATACTGTTTCTACAAAATATTCCTTATAAAAACAAAGAACAAAAATTGAATATTTA ATGAATTGACATTTTATAACCAACCTGTTTTTATCTACGGTGGGAATCTTTGATGCCAGAAATTTATAAAGAGGTTCTGTATCTTCACACCTTGAATAAG CATAATACCATAAAAAATGACACTTGACATGTCAATGTATTTGTCATTTCATTTTAAACTCGTATTTGTGGTTTTTTTCCCAGATAAAAATGAAATTAAA CCATTTCTTTTTAAGAAA

refseq_PDE8A.F1 (forward primer)
refseq_PDE8A.R1 (reverse primer)
Single exon skipping

Frame 0

PPPPQRPHRDKRGGRVS*REIRARRRPPRRR*H GARGGPEAPGGPFADRIAATRQRVRGAAASMGCA PSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQ KTAALPRTRGAGLLESELRDGSGKKVAVADVQF GPMRFHQDQLQVLLVFTKEDNQCNGFCRACEKAG FKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQ LDAEALCRSIRSSKLSENTVIVGVVRRVDREEL SVMPFISAGFTRRYVENPNIMACYNELLQLEFGE VRSQLKLRACNSVFTALENSEDAIEITSEDRFI QYANPAFETTMGYQSGELIGKELGEVPINEKKA DLLDTINSCIRIGKEWQGIYYAKKKNGDNIQQNV KIIPVIGQGGKIRHYVSIIRVCNGNNKAEKISE CVQSDTHTDNQTGKHKDRRKGSLDVKAVASRAT EVSSQRRHSSMARIHSMTIEAPITKVINIINAAQ ESSPMPVTEALDRVLEILRTTELYSPQFGAKDD DPHANDLVGGLMSDGLRRLSGNEYVLSTKNTQM VSSNIITPISLDDVPPRIARAMENEEYWDFDIFE LEAATHNRPLIYLGLKMFARFGICEFLHCSEST LRSWLQIIEANYHSSNPYHNSTHSADVLHATAY FLSKERIKETLDPIDEVAALIAATIHDVDHPGRT NSFLCNAGSELAILYNDTAVLESHHAALAFQLT TGDDKCNIFKNMERNDYRTLRQGIIDMVLATEM TKHFEHVNKFVNSINKPLATLEENGETDKNQEVI NTMLRTPENRTLIKRMLIKCADVSNPCRPLQYC IEWAARISEEYFSQTDEEKQQGLPVVMPVFDRN TCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQ HLDNNFKYWKGLDEMKLRNLRPPPE*WETPPRA LKLCSFGHLEFLRAARAPWSFQY*AEQPPICIA CESPRGHQ*PSAATIQCHYCQVRLGHCSLGLLQE LFRKAHEDHGLPQFLVKHKVWSAPAKGIDGLPA SDRACLLQTILSVTFST*ERLMAIGSLATFSHH RRCNRSLGNTTESDFSFKIE*QMKN*NLNLIINI N*NVLFILLKAQYFL*IQKYFRAKANFKYRDQI YMIHIHYALLGIQTYFHNAN**NDTFTAL*KYS CMLNFSD*G*LEKAGVVF*VLKKAASTV*NPGL* NSSSRLMHFTGNSPR*N*TLLLVRKEKLGLNTF F*NFIIQFPKVYSILYCFYKIFLIKTKNKN*IF NELTFYNQPVFIYGGNL*CQKFIKRFCIFTP*IS IIP*KMTLDMSMYLSFHFKLVFVVFFPDKNEIK PFLFKK

Frame 1

RRRRSARTAIKGAAAFPDARSALAAARPGGDDT APAAARRRRVGRLLTGSRLPASVSAAPPPAWAV PRASTFPSAWWPRTRLAPRHRRCRPAGRASRRAR RRPPCPGPAAPASWSRSFATAAARR*Q*LMCSL AP*DFIKINFRYF*CLPKKITNVMDSAGHVKKQ GLSVQLPRRLRLSLPVSWTNIMTLSS*TTEILDS WMQRHCAGLSDHQNSQKTQLLLV*YAGWIEKSC P*CLSFLLDLQGGM*KTPTSWPATMNCSSWSLE RCDHN*NSGLVTQYSLH*KTVKMQLKLQAKTVLY SMQILHLKQQWAISQVN**GRS*EKCL*MKKRL TCSIL*IHASG*ARSGKEFTMPKRKTEIIYNKM *R*YLSLDREEKLDTMCPLSECAMATIRLRKYPN VFSLTLIQIIRQANIKTGEKAH*TSKLLPPVQL KFPARDDTLPWPGYIP*QLRRPSPR*SILSMLP RKVVPCL*QKP*TVCWKF*EPLSYIHHSLVLKMM IPMPMTLLGA*CLMVCEDYQGMNMFFQQKTLKW FQAI*SLPSPLMMSHHG*LGPWKMRNTGTLIFL NWRLPPTIGL*FILVSKCLLALESVNSYTAPSQR *DHGYKLSKPIIIPPIPTTILHILLMCFMPLPI FSPRRG*RKL*IQLMRSLHSSQPPFMMWITLGE PTPSCVMLEVSWPFCTMTLLCWRATMRPWPSS*P LEMINAIYLKTWRGMIIGHCARGLSTWS*PQK* QSTLSMSTNLSTASTNPWQH*KKMGKLIKTRK* *TLCLGLQRTGP*SNEC*LNVLMCPIPADPCSTA SSGLHAFRKNIFLRLMKRSSRAYLW*CQCLTEI PAASPNPKSLSLITSSQTCLMLGMPL*TCLI*C SILTTTLNTGKDWTK*SCGTSDHLLNSGRHHPEP *SFVPSVIWNS*GQPELLGPFSTRQNSPRSA*P VKAHGDISNLLQPPSNAITVK*DLATVAWACCR SSSERHMRTTVCLSFW*NTRSGVPLQRVLMDFLP VTEHVYCKQFSQLRSALKNG*WQ*DL*QLFHII EGAIAHLGTLLRVTSLLKLSSR*KIKI*T*LLI SIKMFYLFY*KLNIFYEFKNTSEPKPTSNTVTKF T*FIFIMHYLVYRLIFIMQINKMTLLLHYRNIH VC*TFLIEANWKKLGSYSKC*RRLLLLYRTQGS ETALAA*CTSQVTPQGKTRLSCWFAKKS*DLTLF SKIL*FNFQKSTLFYTVSTKYSL*KQRTKIEYL MN*HFITNLFLSTVGIFDARNL*RGSVSSHLE* A*YHKK*HLTCQCICHFILNSYLWFFSQIKMKLN HFFLR

Frame 2

AAAAAPAPR*KGRPRFLTRDPRSPPPAQAAMTR RPRRPGGAGWAVC*PDRGYPPACPRRRRQHGLC PEHPHFRAPGGRGRA*PRGTAAVVRRAAPPAGP EDGRLAPDPRRRPLGVGASRRQRQEGSSS*CAVW PHEISSRSTSGTFSVYQRR*PM*WILQGM*KSR V*VYSYQGGSGCPCLFPGQTS*HYHHRPQKSST AGCRGTVQVYQIIKTLRKHSYCWCSTQGG*RRVV RNAFHFCWIYKEVCRKPQHHGLLQ*TAPAGVWR GAITTETQGL*LSIHCIRKQ*RCN*NYKRRPFY TVCKSCI*NNNGLSVR*INREGVRRSAYK*KKG* LARYYKFMHQDRQGVARNLLCQKEKRR*YTTKC EDNTCHWTGRKN*TLCVHYQSVQWQQ*G*ENIR MCSV*HSYR*SDRQT*RQEKRLTRRQSCCLPCN* SFQPETTLFHGPDTFHDN*GAHHQGNQYYQCCP GK*SHACDRSPRPCAGNSKNH*VIFTTVWC*R* *SPCQ*PCWGLNV*WFAKTIRE*ICSFNKKHSNG FKQYNHSHLP**CPTTDSSGHGK*GILGL*YF* TGGCHPQ*AFDLSWSQNVCSLWNL*ILTLLRVN AKIMVTNYRSQLSFLQSLPQFYTFC*CASCHCLF SLQGEDKGNFRSN**GRCTHRSHHS*CGSPWEN QLLPV*CWK*AGHFVQ*HCCAGEPPCGLGLPAD HWR**MQYI*KHGEE*LSDTAPGDYRHGLSHRND KAL*ACQQICQQHQQTLGNTRRKWGN**KPGSD KHYA*DSREPDPNQTNAD*MC*CVQSLPTPAVL HRVGCTHFGRIFFSD**REAAGLTCGDASV*QKY LQHPQIPNLFH*LLHHRHV*CLGCLCRPA*FNA AS*QQL*ILERTGRNEAAEPPTTS*IVGDTTQS PEALFLRSFGIPEGSQSSLVLSVLGRTAPDLHSL *KPTGTSVTFCSHHPMPLLSSETWPL*PGPAAG ALQKGT*GPRFASVSGKTQGLECPCKGY*WTSC Q*QSMSIANNSLSYVQHLRTANGNRIFSNFFTS* KVQSLTWEHY*E*LLF*N*VADEKLKFELDY*Y QLKCFIYFIKSSIFSMNSKILQSQSQLQIP*PN LHDSYSLCITWYTDLFS*CKLIK*HFYCTIEIFM YVKLF*LRLTGKSWGRILSAKEGCFYCIEPRAL KQL*PPNALHR*LPKVKLDSLVGSQRKVRT*HF FLKFYNSISKSLLYFILFLQNIPYKNKEQKLNI* *IDIL*PTCFYLRWESLMPEIYKEVLYLHTLNK HNTIKNDT*HVNVFVISF*TRICGFFPR*K*N* TISF*E

Sequence NM_173457

CCGCCGCCGCCGCAGCGCCCGCACCGCGATAAAAGGGGCGGCCGCGTTTCCTGACGCGAGATCCGCGCTCGCCGCCGCCCGCCCAGGCGGCGATGACACG GCGCCCGCGGCGGCCCGGAGGCGCCGGGTGGGCCGTTTGCTGACCGGATCGCGGCTACCCGCCAGCGTGTCCGCGGCGCCGCCGCCAGCATGGGCTGTGC CCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGCCCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAG AAGACGGCCGCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGCAAGAAGGTAGCAGTAGCTGATGTGCAGTTTG GCCCCATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGTGAAAAAGCAGG GTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTCCTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAG CTGGATGCAGAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGTGTAGTACGCAGGGTGGATAGAGAAGAGTTGT CCGTAATGCCTTTCATTTCTGCTGGATTTACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTGGAGTTTGGAGA GGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCATTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATA CAGCCCTGTTGTAGCAGCTCCTGGTTTGGCGCTGTGCACATCCCTTCCTCTGCCCCTGTTGAAGTGGGAGTGGGCCTGCTGCCCTCCTGGAGCCTCAGGA GAACATAGCAGAAAGCCCAGCCGGGTATCACCCAAGCAGAAGCTCGTCTGGGCTCCAGAATTCTTCGTGGGTTATGTGCTTATGGCTTCACAACTGCCAC GTATGCAAATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGAGTTAGGAGAAGTGCCTATAAATGAAAAAAAGGCTGAC TTGCTCGATACTATAAATTCATGCATCAGGATAGGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGA AGATAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGCAATGGCAACAATAAGGCTGAGAAAATATCCGAATG TGTTCAGTCTGACACTCATACAGATAATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCCTCCCGTGCAACTGAA GTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATCAATGCTGCCCAGG AAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTAAGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGA TCCCCATGCCAATGACCTTGTTGGGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAAAACACTCAAATGGTT TCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCACGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGAAC TGGAGGCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACGCT AAGATCATGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCTGATGTGCTTCATGCCACTGCCTATTTT CTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATGAGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCA ACTCCTTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTGGCCTTCCAGCTGACCAC TGGAGATGATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTAGCCACAGAAATGACA AAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAAGTGATAA ACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTGATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCAT CGAGTGGGCTGCACGCATTTCGGAAGAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTGTTTGACAGAAATACC TGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCAGC ATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCACCTCCTGAATAGTGGGAGACACCACCCAGAGCCCT GAAGCTTTGTTCCTTCGGTCATTTGGAATTCCTGAGGGCAGCCAGAGCTCCTTGGTCCTTTCAGTACTAGGCAGAACAGCCCCCGATCTGCATAGCCTGT GAAAGCCCACGGGGACATCAGTAACCTTCTGCAGCCACCATCCAATGCCATTACTGTCAAGTGAGACTTGGCCACTGTAGCCTGGGCCTGCTGCAGGAGC TCTTCAGAAAGGCACATGAGGACCACGGTTTGCCTCAGTTTCTGGTAAAACACAAGGTCTGGAGTGCCCCTGCAAAGGGTATTGATGGACTTCCTGCCAG TGACAGAGCATGTCTATTGCAAACAATTCTCTCAGTTACGTTCAGCACTTAAGAACGGCTAATGGCAATAGGATCTTTAGCAACTTTTTCACATCATAGA AGGTGCAATCGCTCACTTGGGAACACTACTGAGAGTGACTTCTCTTTTAAAATTGAGTAGCAGATGAAAAATTAAAATTTGAACTTGATTATTAATATCA ATTAAAATGTTTTATTTATTTTATTAAAAGCTCAATATTTTCTATGAATTCAAAAATACTTCAGAGCCAAAGCCAACTTCAAATACCGTGACCAAATTTA CATGATTCATATTCATTATGCATTACTTGGTATACAGACTTATTTTCATAATGCAAATTAATAAAATGACACTTTTACTGCACTATAGAAATATTCATGT ATGTTAAACTTTTCTGATTGAGGCTAACTGGAAAAAGCTGGGGTCGTATTCTAAGTGCTAAAGAAGGCTGCTTCTACTGTATAGAACCCAGGGCTCTGAA ACAGCTCTAGCCGCCTAATGCACTTCACAGGTAACTCCCCAAGGTAAAACTAGACTCTCTTGTTGGTTCGCAAAGAAAAGTTAGGACTTAACACTTTTTT CTAAAATTTTATAATTCAATTTCCAAAAGTCTACTCTATTTTATACTGTTTCTACAAAATATTCCTTATAAAAACAAAGAACAAAAATTGAATATTTAAT GAATTGACATTTTATAACCAACCTGTTTTTATCTACGGTGGGAATCTTTGATGCCAGAAATTTATAAAGAGGTTCTGTATCTTCACACCTTGAATAAGCA TAATACCATAAAAAATGACACTTGACATGTCAATGTATTTGTCATTTCATTTTAAACTCGTATTTGTGGTTTTTTTCCCAGATAAAAATGAAATTAAACC ATTTCTTTTTAAGAAA

refseq_PDE8A.F1 (forward primer)
refseq_PDE8A.R1 (reverse primer)
Single exon skipping

Frame 0

PPPPQRPHRDKRGGRVS*REIRARRRPPRRR*H GARGGPEAPGGPFADRIAATRQRVRGAAASMGCA PSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQ KTAALPRTRGAGLLESELRDGSGKKVAVADVQF GPMRFHQDQLQVLLVFTKEDNQCNGFCRACEKAG FKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQ LDAEALCRSIRSSKLSENTVIVGVVRRVDREEL SVMPFISAGFTRRYVENPNIMACYNELLQLEFGE VRSQLKLRACNSVFTALENSEDAIEITSEDRFI QPCCSSSWFGAVHIPSSAPVEVGVGLLPSWSLR RT*QKAQPGITQAEARLGSRILRGLCAYGFTTAT YANPAFETTMGYQSGELIGKELGEVPINEKKAD LLDTINSCIRIGKEWQGIYYAKKKNGDNIQQNV KIIPVIGQGGKIRHYVSIIRVCNGNNKAEKISEC VQSDTHTDNQTGKHKDRRKGSLDVKAVASRATE VSSQRRHSSMARIHSMTIEAPITKVINIINAAQ ESSPMPVTEALDRVLEILRTTELYSPQFGAKDDD PHANDLVGGLMSDGLRRLSGNEYVLSTKNTQMV SSNIITPISLDDVPPRIARAMENEEYWDFDIFE LEAATHNRPLIYLGLKMFARFGICEFLHCSESTL RSWLQIIEANYHSSNPYHNSTHSADVLHATAYF LSKERIKETLDPIDEVAALIAATIHDVDHPGRT NSFLCNAGSELAILYNDTAVLESHHAALAFQLTT GDDKCNIFKNMERNDYRTLRQGIIDMVLATEMT KHFEHVNKFVNSINKPLATLEENGETDKNQEVI NTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCI EWAARISEEYFSQTDEEKQQGLPVVMPVFDRNT CSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQ HLDNNFKYWKGLDEMKLRNLRPPPE*WETPPRAL KLCSFGHLEFLRAARAPWSFQY*AEQPPICIAC ESPRGHQ*PSAATIQCHYCQVRLGHCSLGLLQE LFRKAHEDHGLPQFLVKHKVWSAPAKGIDGLPAS DRACLLQTILSVTFST*ERLMAIGSLATFSHHR RCNRSLGNTTESDFSFKIE*QMKN*NLNLIINI N*NVLFILLKAQYFL*IQKYFRAKANFKYRDQIY MIHIHYALLGIQTYFHNAN**NDTFTAL*KYSC MLNFSD*G*LEKAGVVF*VLKKAASTV*NPGL* NSSSRLMHFTGNSPR*N*TLLLVRKEKLGLNTFF *NFIIQFPKVYSILYCFYKIFLIKTKNKN*IFN ELTFYNQPVFIYGGNL*CQKFIKRFCIFTP*IS IIP*KMTLDMSMYLSFHFKLVFVVFFPDKNEIKP FLFKK

Frame 1

RRRRSARTAIKGAAAFPDARSALAAARPGGDDT APAAARRRRVGRLLTGSRLPASVSAAPPPAWAV PRASTFPSAWWPRTRLAPRHRRCRPAGRASRRAR RRPPCPGPAAPASWSRSFATAAARR*Q*LMCSL AP*DFIKINFRYF*CLPKKITNVMDSAGHVKKQ GLSVQLPRRLRLSLPVSWTNIMTLSS*TTEILDS WMQRHCAGLSDHQNSQKTQLLLV*YAGWIEKSC P*CLSFLLDLQGGM*KTPTSWPATMNCSSWSLE RCDHN*NSGLVTQYSLH*KTVKMQLKLQAKTVLY SPVVAAPGLALCTSLPLPLLKWEWACCPPGASG EHSRKPSRVSPKQKLVWAPEFFVGYVLMASQLP RMQILHLKQQWAISQVN**GRS*EKCL*MKKRLT CSIL*IHASG*ARSGKEFTMPKRKTEIIYNKM* R*YLSLDREEKLDTMCPLSECAMATIRLRKYPN VFSLTLIQIIRQANIKTGEKAH*TSKLLPPVQLK FPARDDTLPWPGYIP*QLRRPSPR*SILSMLPR KVVPCL*QKP*TVCWKF*EPLSYIHHSLVLKMM IPMPMTLLGA*CLMVCEDYQGMNMFFQQKTLKWF QAI*SLPSPLMMSHHG*LGPWKMRNTGTLIFLN WRLPPTIGL*FILVSKCLLALESVNSYTAPSQR *DHGYKLSKPIIIPPIPTTILHILLMCFMPLPIF SPRRG*RKL*IQLMRSLHSSQPPFMMWITLGEP TPSCVMLEVSWPFCTMTLLCWRATMRPWPSS*P LEMINAIYLKTWRGMIIGHCARGLSTWS*PQK*Q STLSMSTNLSTASTNPWQH*KKMGKLIKTRK** TLCLGLQRTGP*SNEC*LNVLMCPIPADPCSTA SSGLHAFRKNIFLRLMKRSSRAYLW*CQCLTEIP AASPNPKSLSLITSSQTCLMLGMPL*TCLI*CS ILTTTLNTGKDWTK*SCGTSDHLLNSGRHHPEP *SFVPSVIWNS*GQPELLGPFSTRQNSPRSA*PV KAHGDISNLLQPPSNAITVK*DLATVAWACCRS SSERHMRTTVCLSFW*NTRSGVPLQRVLMDFLP VTEHVYCKQFSQLRSALKNG*WQ*DL*QLFHIIE GAIAHLGTLLRVTSLLKLSSR*KIKI*T*LLIS IKMFYLFY*KLNIFYEFKNTSEPKPTSNTVTKF T*FIFIMHYLVYRLIFIMQINKMTLLLHYRNIHV C*TFLIEANWKKLGSYSKC*RRLLLLYRTQGSE TALAA*CTSQVTPQGKTRLSCWFAKKS*DLTLF SKIL*FNFQKSTLFYTVSTKYSL*KQRTKIEYLM N*HFITNLFLSTVGIFDARNL*RGSVSSHLE*A *YHKK*HLTCQCICHFILNSYLWFFSQIKMKLN HFFLR

Frame 2

AAAAAPAPR*KGRPRFLTRDPRSPPPAQAAMTR RPRRPGGAGWAVC*PDRGYPPACPRRRRQHGLC PEHPHFRAPGGRGRA*PRGTAAVVRRAAPPAGP EDGRLAPDPRRRPLGVGASRRQRQEGSSS*CAVW PHEISSRSTSGTFSVYQRR*PM*WILQGM*KSR V*VYSYQGGSGCPCLFPGQTS*HYHHRPQKSST AGCRGTVQVYQIIKTLRKHSYCWCSTQGG*RRVV RNAFHFCWIYKEVCRKPQHHGLLQ*TAPAGVWR GAITTETQGL*LSIHCIRKQ*RCN*NYKRRPFY TALL*QLLVWRCAHPFLCPC*SGSGPAALLEPQE NIAESPAGYHPSRSSSGLQNSSWVMCLWLHNCH VCKSCI*NNNGLSVR*INREGVRRSAYK*KKG* LARYYKFMHQDRQGVARNLLCQKEKRR*YTTKCE DNTCHWTGRKN*TLCVHYQSVQWQQ*G*ENIRM CSV*HSYR*SDRQT*RQEKRLTRRQSCCLPCN* SFQPETTLFHGPDTFHDN*GAHHQGNQYYQCCPG K*SHACDRSPRPCAGNSKNH*VIFTTVWC*R** SPCQ*PCWGLNV*WFAKTIRE*ICSFNKKHSNG FKQYNHSHLP**CPTTDSSGHGK*GILGL*YF*T GGCHPQ*AFDLSWSQNVCSLWNL*ILTLLRVNA KIMVTNYRSQLSFLQSLPQFYTFC*CASCHCLF SLQGEDKGNFRSN**GRCTHRSHHS*CGSPWENQ LLPV*CWK*AGHFVQ*HCCAGEPPCGLGLPADH WR**MQYI*KHGEE*LSDTAPGDYRHGLSHRND KAL*ACQQICQQHQQTLGNTRRKWGN**KPGSDK HYA*DSREPDPNQTNAD*MC*CVQSLPTPAVLH RVGCTHFGRIFFSD**REAAGLTCGDASV*QKY LQHPQIPNLFH*LLHHRHV*CLGCLCRPA*FNAA S*QQL*ILERTGRNEAAEPPTTS*IVGDTTQSP EALFLRSFGIPEGSQSSLVLSVLGRTAPDLHSL *KPTGTSVTFCSHHPMPLLSSETWPL*PGPAAGA LQKGT*GPRFASVSGKTQGLECPCKGY*WTSCQ *QSMSIANNSLSYVQHLRTANGNRIFSNFFTS* KVQSLTWEHY*E*LLF*N*VADEKLKFELDY*YQ LKCFIYFIKSSIFSMNSKILQSQSQLQIP*PNL HDSYSLCITWYTDLFS*CKLIK*HFYCTIEIFM YVKLF*LRLTGKSWGRILSAKEGCFYCIEPRALK QL*PPNALHR*LPKVKLDSLVGSQRKVRT*HFF LKFYNSISKSLLYFILFLQNIPYKNKEQKLNI* *IDIL*PTCFYLRWESLMPEIYKEVLYLHTLNKH NTIKNDT*HVNVFVISF*TRICGFFPR*K*N*T ISF*E