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Sequences

Sequence MAP4K4.iAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACC ACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACA TCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGC CCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCC TGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCT GAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAG TAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGA GGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATG ACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAG ACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGA TCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCT GAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTC TTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTT GTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAA GTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCT TTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATT CCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAAT ACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGA TGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGT GTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTAT TTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAAT TCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTT ATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTAT AAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAG TGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTA ATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTA TAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGAT TTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAA AAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTA ATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCC TACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGAT TGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGA AACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTG GTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCA ATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCG TTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTT TCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCT TGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGT TAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGG CACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCA CTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGAT GTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAG GGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAA CATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCA ATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATG TTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCG AGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACT GAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCA GATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATT TTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACA TTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGT AACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGC ACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTT GCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGC TAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTC GAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGT ATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATG AACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS GTWKSFSSSCSRSRPCYCMTIGGRTRSTRSSRH HRSRKGASQASMLPSPKPTTSLLTERERFL*EQH LAPLFCPVEIPHCRAVGSRIARQDRETPPAVLS PGFCGREWRSWCPDLAVAAPQGPATQDPSPGLT LGLRVAPGNASE*DHHPSLKALHLSAWKMQ*KNL KIKRKFSDPSSLLI*PHWPKSFEQWKMYGHLTK *RTTPHPVRSRGRRMRRTTMWSRKGLTSPPQDQ RTPEQRHL*I*AMVKRNL*KP*LSMMM*KVSRP* PHPRRAL*SSARLSPLVAHSRNTNLPPPLHLL* TPDYYRFLHLAEQQ*HLWWDFPVMG*DQKP*GK ILPGKAQWSM*ILPTLGHRVTPRRFVNTRRGLTL RFCVLPYGE*IC*WVQRVA*CCWTEVAKGRSIL LSTEDDFNKWTYLRA*MSW*QYLAKRISYVSTI CPG*EIKYFTMIQKLRRSRDGQP*GIWKDVYIIK L*NMKESNFW*LL*RVLWKSMRGHQSHITNLWP LSHLENWYISHYWWISLLRKARG*K*SMDPVLD SMLLMWIQDQSMTFIYQHISSVASNPMQSSSSPI QMEWSFWCAMKMRGFM*THMEGSPRM*FYSGER CLHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMV CSCTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFI S*P*AGLLF*AGRSSVIQGLLASRVFKILRTWN SL*LELGAAPRATRTAVCADLMCWVLSPPSCSS YIPVYPHSFFFSYSKINQGCNAAGAVQILPSGAI SVWD*ASYWKANTFPPAPEFLVSE*LCLVGV*Q WRR*TCRWFYWQWAQGGEKWW*KERSAEAESRF NIVTLTVYLIDISFL*CDDMWTKKKMQV*EVNIY KM*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLD LGWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKK KKGKKKRKRKRVHFV*WPELCRVTVLALPQLKV* FLFKPRRATKVLPQSALAEERKKFKPHVYFFFA YLIVLNALRGDLVYMFLEETINV*FNLNTKTTR LKFDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLE TRMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGL VADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KC NALKLF*ECS*FCLFIKWASSVF*V*YRSPGFSR FLTCSLVLCFFKPILLYEYIHDICNKCLEKEFV SWLHGHHSSSRKDITVSGKDQDSMSQSCHLTFL LSSSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVV NFPVYCENGGFSLCSHPPKHSSHLYLFASHPTW HPL*PLVSFLFLVPSF*KRPSFELQTSGKTSTS THECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWM FHLYEFGAVLKAKAAYWFVVNLEKVEKLILPLK GFEVGWIPSPQDFS*N*IPACNLYPCLGFS*DE TCHSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQ CLMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQM LHKKKCKWCFIGITKCLELAGFPIGGKGV*AHT ELCLACLCSSRHPVGTLVVFVVN*IESIVGLKLL KLEHPLSFSAGASWSVLYLAFFLPVLASPPCQP D*VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNY FPPFYRVVTSPQKCQYGKITQENSIAMTPLHKEH *TVRGVLFQTKQKCL*V*SELSQVITFLY*NQS NPFCIECSVFYSFLMHMLRWRKCLQKWFCYTNA HHIFMVYFK*LFMGYLGFRLSCSTLTLILPIINL LNSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC *LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEF RICSDPSECFLPVRIHILCIPAYPELPVPEQAPV FCYFR*QGLAQGQV*ITKVANVCLKTSEMKWEM NLHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY VATKERAGS*KAAGT*TAKERTRRKEASRGVGE KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA ALGSPSAAAAPGAGHVTA*P*EAAPAALAAAAT TAAGKEQAKLPCSRAQSPLRAC*PSARGSCENN ISLPCSVPSRFPTAGQWAAE*PGRTEKLHQQY*A QASVGESGEAGAQTWQWQLLRVQQLRIPARVSP WVSEWLRGTLQSEIIIQV*RLSISAPGKCSEKT *R*KGSFQTPQAC*SDRTGQRASSSGRCTATSQS NGLLLIQ*GVGDDG*GGRRCGAGRG*RVHLRTR GHQSSVISEFEQW*NGICENHDCP**CRK*AGH DPIQGGHSNRPPDSVR**HTPETQIFLLLYTFYR PQITTDFSI*RNNSDICGGIFL*WDETRSHKAR SYPERLSGQCESYQH*ATE*HPGDS*IQEEV*L *DSVCCLMGSEFASGYREWPDAAGQKWPREGLSS YQPKTISTNGRT*GLECLGDNIWQKG*VTCLLF VLVKK*NTSQ*SRS*EEAGMDNRRGFGRMCTL* SCKI*KNQISGDCFEEFCGSLCVGTKAISQIYGL *VIWRIGT*AITGGSHC*GRPEVESDLWILCWI PCC*CGFRISL*HLSTNTYPV*HQTPCNHHPPQ YRWNGASGVL*R*GGLCKHIWKDHQGCSSTVGRD AYISSIYSIQSDNGLGREGHRDPICGNWSLGWC VHAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGL FHDLRQDFSSELVEAV*SRDYWPPESSRS*ELGI PCNWSSELHRGQPGQLCVQTSCVGFSPLLPVPL IYQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL *VFGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDS GDDEHAVGFIGSGHKEVRSGGKRSGVLKQRADL I**H*QCI*LTFLFCNVTICGQRRRCRFKKLIF IKCERHSY*DNFFVGGAWEMGWGGLRGPILFLWI WGGGPGQELSHFSVYQVA*IMCRWF*KKKKKKK KKEKKKEKENVCILYNGQNFVV*QY*HCLS*RF NFCLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLP TSLFLMH*EVI*FICFWKKPLMFNLILIPKRPD *SLTFIVTNQQAQELSMKMGNSLKADAVYLQV* KPECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW *QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNA MH*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFL VFSLALLFSVFLNQFYFMNIFMTFVINVLRKNLF HGFMVITQAPVRILPSQERIRTPCHSPAILLSS CRVLSGNNCIMAALTSVIKRNLLFFRLDLFPLW LIFLYIVKMGDFPSAPTHLNTAAICTCLLPIPLG THSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHP LMNADTAAGGFVYTCKDKLRSLLFGEVKEDGNG CFICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*R DLR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMK HATVNTLVYISREEGKAQVENSKRWADVFEEML NV*C*VGEGREQRMWHNGLNIIQRLEAPCL*VKC YTKKNANGVSLELPSA*NLLAFP*VVKGSELTP SCAWLACAAPGTRWALWWCLW*TELNPLLGLSY *NWNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQ IELL*LDAGTQ*SFSVKDWESSKCLDSFRKGII FPPFTG**LLHRSANMAKLHKKTVLQ*HHYIRN IELLEECSSKQNKNVSRFSQSFHK**PFCIKIRV TLSVLSAVFFTLFSCTCYVGENVYKNGFVTLMR TTYLWFILSDFLWVI*VFVLVVAHLP*FCQLLI C*IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSA NLSTVYYENYCCPPTLFSLNKVKMTPILCGMSF EYVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLL YFAISDDRGLPKARYRLQK*QMFV*RPQR*SGR* TSITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT *QQRREREARRQQEREQRRREQEEKRRLEELER RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ RHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPP PQQERSKPSFHAPEPKAHYEPADRAREVPVRTT SRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIE PRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHP GSQSGSGERFRVRSSSKSEGSPSQRLENAVKKP EDKKEVFRPLKPADLTALAKELRAVEDVRPPHK VTDYSSSSEESGTTDEEDDDVEQEGADESTSGPE DTRAASSLNLSNGETESVKTMIVHDDVESEPAM TPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFI DPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQD PTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNS EILCAALWGVNLLVGTESGLMLLDRSGQGKVYP LINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYL SWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYK VVKYERIKFLVIALKSSVEVYAWAPKPYHKFMA FKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGF HAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPN TDGMELLVCYEDEGVYVNTYGRITKDVVLQWGE MPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGV FMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY FMTLGRTSLLSW*KQCDPGITGLQSLQDPENLE FLVTGARSCTEGNQDSCVCRPHVLGSLPSFLFLL YTSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCY KCLGLSIILESKHLSSSSRIPCL*MTLSCGCLT VATMNMPLVLLAVGTRR*EVVVKGAEC*SREQI* YSNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL *NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFG FGVGVLAKNSVIFLCTRLPKSCADGSKKKKKKKK KRKKKKKKKTCAFCIMARTLSCDSISTASVKGL IFV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFC LPHCS*CIER*FSLYVFGRNH*CLI*S*YQNDQI EV*LLLSQISRHKNCP*RWEIALRLMQFTYKFR NQNALFLPDSPLEVDGATAAHDTRSHCSRCRGV GSRQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQ CTKTILRM*LILLIHKVGIFCVLGVISKSWLFS FSHLLSCSLFF*TNFTL*IYS*HL**MS*ERIC FMASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPL VEF*VEITALWLL*PQSSKETCCFLGLIFFLCG *FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHL APTLTSCQFPVPGSIFLKKALL*ATNIW*DKYIH S*MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMD VSFV*VWSSAEGQSRLLVCS*PREG*KINPTFK GI*GRLDSIATGL*LELNSCL*FISMFRLFIR*N MPQ*THSCTYQEKKERHRWRTVKGGQMSLKKCS MSDAKWEKAENKGCGIMVLTLSKDLKLHVCKSN VTQKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHR VVLGLLVQLQAPGGHSGGVCGELN*IHCWA*VT EIGTPFVLLGGGFLVCALLGFFPSRLSLTPLST RLSCYSLMQGPSEVSPLKIGSRRNV*ILLGKELF SPLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GT LNC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE *PFLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CA PHIYGLF*VTFYGLFRFSS*L*HTYPNFANY*F AK**YK*QTALNLLIIKAAKQTGGKYTALFFAV LTCLLCIMKITVVPPPFFP*IK*K*HLFYVA*VS NMF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSC ILLFQMTGACPRPGIDYKSSKCLSKDLRDEVGD EPPSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Sequence MAP4K4.hAug05

GAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCAT TACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATT CATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACA GGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAG AAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCC AGAAACCCTCCTCCCCGGCTGAAGTCAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTA CAGAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGC CTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTC CGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAG CAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAG AAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTA TATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAG ATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGC AGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCG AGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGA AACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGAT CCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGA AAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGAT GTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACG AGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGT AGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCC TCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGAC CAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATA CAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGC CAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATA AGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGA AGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCA TATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCT ATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGC AATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTA GTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAA CTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGG TGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAA GATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCC CCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGA TTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTG TCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCT GAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGG TTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTC CCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAA AAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCAC TGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACA TGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTT AATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCA GTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGT TCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTT TCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATC GAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATG TCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCC TGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTT GATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTT TGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCT GGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTA AAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAA ATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTA GGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGAT GTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTC CATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAA GGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCA TTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGC CTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTC TTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGA CACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTT TCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGG TTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAA TTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACA GCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTAT TTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCG TACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTC AGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAAT TTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

ETLQHIMVLSSKRALQDMMTNSGLLWSSVGLGP LQTL*RTPKGTHSKKTGSLTSPEKS*GDWHIFTF IM*FTGISRARMCC*LRMQR*NLLTLV*VLSWT GLWGGEIRS*ALPTGWLLRSSPVMRTQMPPMIT EVIFGLVALQPLRWQKVLPLSVTCIQ*EHCFSFP ETLLPG*SQKNGRRSFLVL*KGAW*RITCSGPL QSSF*NILL*GISQMKDETEYEYSGSEEEEEEV PEQEGEPSSIVNVPGESTLRRDFLRLQQENKERS EALRRQQLLQEQQLREQEEYKRQLLAERQKRIE QQKEQRRRLEEQQRREREARRQQEREQRRREQE EKRRLEELERRRKEEEERRRAEEEKRRVEREQEY IRRQLEEEQRHLEVLQQQLLQEQAMLLECRWRE MEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHP QHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAR EVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQR NSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSG SQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE NAVKKPEDKKEVFRPLKPADLTALAKELRAVED VRPPHKVTDYSSSSEESGTTDEEDDDVEQEGAD ESTSGPEDTRAASSLNLSNGETESVKTMIVHDDV ESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSS SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMR PEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKY KKRFNSEILCAALWGVNLLVGTESGLMLLDRSG QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKD KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHA IIILPNTDGMELLVCYEDEGVYVNTYGRITKDV VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVE TGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSG GSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSL PSFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCSD STIRCYKCLGLSIILESKHLSSSSRIPCL*MTL SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC *SREQI*YSNINSVFN*HFFFVM*RYVDKEEDAG LRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSK KKKKKKKKRKKKKKKKTCAFCIMARTLSCDSIST ASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S *YQNDQIEV*LLLSQISRHKNCP*RWEIALRLMQ FTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGS FPFLKMQCTKTILRM*LILLIHKVGIFCVLGVIS KSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTV LPSYFPLVEF*VEITALWLL*PQSSKETCCFLGL IFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNI W*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK* KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K INPTFKGI*GRLDSIATGL*LELNSCL*FISMF RLFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQM SLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW* RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN*IH CWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*I LLGKELFSPLLQGSNFSTEVPIWQNYTRKQYCND TIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKM VLLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYPN FANY*FAK**YK*QTALNLLIIKAAKQTGGKYT ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HL FYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*ASR TRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFE FGSKN

Frame 1

KHCNILWCFHQKEPSRT**PTLACYGVLWGWVH YRPCEEHQREHTQRRLDRLHLQRNPEGTGTSSH SSCDSPGYQGPECVAD*ECRGETC*LWCECSAGQ DCGAEKYVHRHSLLDGS*GHRL**EPRCHL*LQ K*SLVLWHYSH*DGRRCSPSL*HASNESTVSHS QKPSSPAEVKKMVEEVF*FYRRVPGEELHAAALY RAAFETSFYKGSAK*KMKLSMSTVGVRKKRRKC LNRKESQVPL*TCLVSLLFAEIS*DCSRRTRNV PRLFGDNSYYRSNSSGSRKNIKGNCWQRDRSGLS SRKNRGDG*KSNKGESGKLEGSRNVNSEGENKK KRGV*RSWREGAKKKRRGDGQKKKRGELKENRS ISGDS*KRSSGTWKSFSSSCSRSRPCYWSADGGR WRSTGRQRGSRGSCNKNKHISCLYSMTIGGRTR STRSSRHHRSRKGASQASMLPSPKPTTSLLTER ERFL*EQHLAPLFCPVEIPHCRAVGSRIARQDRE TPPVLSPGFCGREWRSWCPDLAVAAPQGPATQD PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAW KMQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQWKM YGHLTK*RTTPHPVRSRGRRMRRTTMWSRKGLT SPPQDQRTPEQRHL*I*AMVKRNL*KP*LSMMM *KVSRP*PHPRRAL*SSARLSPLVAHSRNTNLPP PLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVN TRRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEVA KGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIW KDVYIIKL*NMKESNFW*LL*RVLWKSMRGHQSH ITNLWPLSHLENWYISHYWWISLLRKARG*K*S MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPM QSSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM* FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGL VAAVRFIS*P*AGLLF*AGRSSVIQGLLASRVFK ILRTWNSL*LELGAAPRATRTAVCADLMCWVLS PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQ ILPSGAISVWD*ASYWKANTFPPAPEFLVSE*LC LVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQ V*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKGS HFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLA LPQLKV*FLFKPRRATKVLPQSALAEERKKFKPH VYFFFAYLIVLNALRGDLVYMFLEETINV*FNL NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*C SLLTSLETRMLCFYQIHH*RLMGQLQPMTQDLIV LDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*Y RSPGFSRFLTCSLVLCFFKPILLYEYIHDICNKC LEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A *SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYLF ASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGS KRRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVEK LILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGR CL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*SS MSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK GV*AHTELCLACLCSSRHPVGTLVVFVVN*IES IVGLKLLKLEHPLSFSAGASWSVLYLAFFLPVLA SPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAM TPLHKEH*TVRGVLFQTKQKCL*V*SELSQVITF LY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTL ILPIINLLNSNTNDKLH*IY*L*KLQSRLVASTQ PFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY FMWHEFRICSDPSECFLPVRIHILCIPAYPELP VPEQAPVFCYFR*QGLAQGQV*ITKVANVCLKTS EMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN LDQKI

Frame 2

NIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSI TDLVKNTKGNTLKEDWIAYISREILRGLAHLHI HHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLD RTVGRRNTFIGTPYWMAPEVIACDENPDATYDYR SDLWSCGITAIEMAEGAPPLCDMHPMRALFLIP RNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPS TEQLLKHPFIRDQPNER*N*V*VQWE*GRRGGSA *TGRRAKFHCERAW*VYSSPRFPETAAGEQGTF RGSSETTVTTGATAPGAGRI*KATAGRETEAD* AAERTEATARRATKERAGS*KAAGT*TAKERTRR KEASRGVGEKAQRRRGEETGRRRKEES*KRTGV YQATARRGAAALGSPSAAAAPGAGHVTGVPMAG DGGAPAGREAPEAVATRTSISPVSTA*P*EAAPA ALAAAATTAAGKEQAKLPCSRAQSPLRAC*PSA RGSCENNISLPCSVPSRFPTAGQWAAE*PGRTE KLHQY*AQASVGESGEAGAQTWQWQLLRVQQLRI PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG KCSEKT*R*KGSFQTPQAC*SDRTGQRASSSGR CTATSQSNGLLLIQ*GVGDDG*GGRRCGAGRG*R VHLRTRGHQSSVISEFEQW*NGICENHDCP**C RK*AGHDPIQGGHSNRPPDSVR**HTPETQIFL LLYTFYRPQITTDFSI*RNNSDICGGIFL*WDET RSHKARSYPERLSGQCESYQH*ATE*HPGDS*I QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKW PREGLSSYQPKTISTNGRT*GLECLGDNIWQKG* VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKA ISQIYGL*VIWRIGT*AITGGSHC*GRPEVESDL WILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGC SSTVGRDAYISSIYSIQSDNGLGREGHRDPICGN WSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESS RS*ELGIPCNWSSELHRGQPGQLCVQTSCVGFSP LLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDS VLWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGVL KQRADLI**H*QCI*LTFLFCNVTICGQRRRCR FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRG PILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*K KKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNH MFIFFLPTSLFLMH*EVI*FICFWKKPLMFNLIL IPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISL FSM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGEF PFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVIN VLRKNLFHGFMVITQAPVRILPSQERIRTPCHSP AILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTC LLPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKHL VRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLK N*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV* AFHKMKHATVNTLVYISREEGKAQVENSKRWAD VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEA PCL*VKCYTKKNANGVSLELPSA*NLLAFP*VVK GSELTPSCAWLACAAPGTRWALWWCLW*TELNP LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS* PHPLVNQIELL*LDAGTQ*SFSVKDWESSKCLDS FRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ* HHYIRNIELLEECSSKQNKNVSRFSQSFHK**P FCIKIRVTLSVLSAVFFTLFSCTCYVGENVYKNG FVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP* FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVH SPFFCSANLSTVYYENYCCPPTLFSLNKVKMTPI LCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RP QR*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*I WIKK

Sequence MAP4K4.gAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGA ACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAG GCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGC AGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAA GAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATC AGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGG AGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCA CTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAG GTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACT CCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATC CCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAA AATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAG TGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGG GGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCAT GATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAAT CTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGG GATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATT CGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACA GAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAA AAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGG GATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCAC CAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAA AGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAA CCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCA AGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATC TGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTT CGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGA GTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGG TTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGC TGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAA TGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGC GGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAA GATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTT AAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGG TTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGT ATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTC AAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAAT TTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGG CTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGAT CTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTG GGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGT GTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGT AATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGT CACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTT TTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTG TACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTAC AAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTG GGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAG GTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATC CATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGT GGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACT TGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAG GTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAAT TGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCC GTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGT TTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTAT TGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTA ATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTAC AAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACT TACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCA AGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATG ACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGA GCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTA AAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAG TTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGET**N*V*VQWE*GRRGGSA* TGRRAKFHCERAW*VYSSPRFPETAAGEQGTFR GSSETTVTTGATAPGAGRI*KATAGRETEAD*AA ERTEATARRATKERAGS*KAAGT*TAKERTRRK EASRGVGEKAQRRRGEETGRRRKEES*KRTGVY QATARRGAAALGSPSAAAAPGAGHVTGVPMAGDG GAPAGREAPEAVATRTSISPVSTA*P*EAAPAA LAAAATTAAGKEQAKLPCSRAQSPLRAC*PSAR GSCENNISLPCSVPSRFPTAGQWAAE*PGRTEKL HQQY*AQASVGESGEAGAQTWQWQLLRVQQLRI PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG KCSEKT*R*KGSFQTPQACWRSGSDRTGQRASSS GRCTATSQSNGLLLIQ*GVGDDG*GGRRCGAGR G*RVHLRTRGHQSSVISEFEQW*NGICENHDCP **CRK*AGHDPIQGGHSNRPPDSVR**HTPETQI FLLLYTFYRPQITTDFSI*RNNSDICGGIFL*W DETRSHKARSYPERLSGQCESYQH*ATE*HPGD S*IQEEV*L*DSVCCLMGSEFASGYREWPDAAGQ KWPREGLSSYQPKTISTNGRT*GLECLGDNIWQ KG*VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRR GFGRMCTL*SCKI*KNQISGDCFEEFCGSLCVGT KAISQIYGL*VIWRIGT*AITGGSHC*GRPEVE SDLWILCWIPCC*CGFRISL*HLSTNTYPV*HQ TPCNHHPPQYRWNGASGVL*R*GGLCKHIWKDHQ GCSSTVGRDAYISSIYSIQSDNGLGREGHRDPI CGNWSLGWCVHAQKGSKTKILV*TQ*QGVLCLC SVWWQQSGLFHDLRQDFSSELVEAV*SRDYWPPE SSRS*ELGIPCNWSSELHRGQPGQLCVQTSCVG FSPLLPVPLIYQFIPILFFFLTPK*IKAAMQLV LFRFYHQVL*VFGIEHHTGKQTPFLQLQNSLSLN DSVLWVSDSGDDEHAVGFIGSGHKEVRSGGKRS GVLKQRADLI**H*QCI*LTFLFCNVTICGQRR RCRFKKLIFIKCERHSY*DNFFVGGAWEMGWGGL RGPILFLWIWGGGPGQELSHFSVYQVA*IMCRW F*KKKKKKKKKEKKKEKENVCILYNGQNFVV*Q Y*HCLS*RFNFCLNLDVQQKFYHSLHLQKKEKNS NHMFIFFLPTSLFLMH*EVI*FICFWKKPLMFN LILIPKRPD*SLTFIVTNQQAQELSMKMGNSLK ADAVYLQV*KPECFVFTRFTIRG*WGNCSP*HKI SLFSM*RGW*QTGGYIRIVTQTVQCCRNLFLGV GEFPFSKNAMH*NYFKNVVNSAYS*SGHLLCFR CNIEVLAFLVFSLALLFSVFLNQFYFMNIFMTFV INVLRKNLFHGFMVITQAPVRILPSQERIRTPC HSPAILLSSCRVLSGNNCIMAALTSVIKRNLLF FRLDLFPLWLIFLYIVKMGDFPSAPTHLNTAAIC TCLLPIPLGTHSDLLSVSCSWFHLFEKGPPLSY KHLVRQVHPLMNADTAAGGFVYTCKDKLRSLLF GEVKEDGNGCFICMSLEQC*RPKPPTGL*LT*RR LKN*SYL*RDLR*AGFHRHRTLVRIKFLLVIYI HV*AFHKMKHATVNTLVYISREEGKAQVENSKR WADVFEEMLNV*C*VGEGREQRMWHNGLNIIQRL EAPCL*VKCYTKKNANGVSLELPSA*NLLAFP* VVKGSELTPSCAWLACAAPGTRWALWWCLW*TE LNPLLGLSY*NWNTLCPSRRGLPGLCFTWLFSFP S*PHPLVNQIELL*LDAGTQ*SFSVKDWESSKC LDSFRKGIIFPPFTG**LLHRSANMAKLHKKTV LQ*HHYIRNIELLEECSSKQNKNVSRFSQSFHK* *PFCIKIRVTLSVLSAVFFTLFSCTCYVGENVY KNGFVTLMRTTYLWFILSDFLWVI*VFVLVVAH LP*FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQ VHSPFFCSANLSTVYYENYCCPPTLFSLNKVKM TPILCGMSFEYVLTLQNVSFL*GSIFYAYLPTL SFPYQSRLLYFAISDDRGLPKARYRLQK*QMFV* RPQR*SGR*TSITRFLKTVWSKAGP*RNVTFLQ F*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLDETEYEYSGSEEEEEEVPE QEGEPSSIVNVPGESTLRRDFLRLQQENKERSE ALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQ QKEQRRRLEEQQRREREARRQQEREQRRREQEEK RRLEELERRRKEEEERRRAEEEKRRVEREQEYI RRQLEEEQRHLEVLQQQLLQEQAMLLECRWREM EEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQH SQQPPPPQQERSKPSFHAPEPKAHYEPADRARE VPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRN STSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGS QPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE NAVKKPEDKKEVFRPLKPAGEVDLTALAKELRA VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEG ADESTSGPEDTRAASSLNLSNGETESVKTMIVH DDVESEPAMTPSKEGTLIVRQTQSASSTLQKHK SSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDG MRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEI RKYKKRFNSEILCAALWGVNLLVGTESGLMLLD RSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGK KDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVG DLEGCVHYKVVKYERIKFLVIALKSSVEVYAWA PKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLK VIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIK PHAIIILPNTDGMELLVCYEDEGVYVNTYGRIT KDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRS VETGHLDGVFMHKRAQRLKFLCERNDKVFFASV RSGGSSQVYFMTLGRTSLLSW*KQCDPGITGLQ SLQDPENLEFLVTGARSCTEGNQDSCVCRPHVLG SLPSFLFLLYTSLSPFFFFFLLQNKSRLQCSWC CSDSTIRCYKCLGLSIILESKHLSSSSRIPCL* MTLSCGCLTVATMNMPLVLLAVGTRR*EVVVKGA EC*SREQI*YSNINSVFN*HFFFVM*RYVDKEE DAGLRS*YL*NVKDTVTRITFLWVGLGRWGGVG *GVPFCFFGFGVGVLAKNSVIFLCTRLPKSCADG SKKKKKKKKKRKKKKKKKTCAFCIMARTLSCDS ISTASVKGLIFV*T*TCNKSFTTVCTCRRKKKI QTTCLFFFCLPHCS*CIER*FSLYVFGRNH*CLI *S*YQNDQIEV*LLLSQISRHKNCP*RWEIALR LMQFTYKFRNQNALFLPDSPLEVDGATAAHDTR SHCSRCRGVGSRQVVTLE*SHKLFSVAGTFSWGW GSFPFLKMQCTKTILRM*LILLIHKVGIFCVLG VISKSWLFSFSHLLSCSLFF*TNFTL*IYS*HL **MS*ERICFMASWSSLKLP*GYYRLRKGSGLHV TVLPSYFPLVEF*VEITALWLL*PQSSKETCCF LGLIFFLCG*FSCIL*KWGIFPLLPPT*TQQPF VPVCFPSHLAPTLTSCQFPVPGSIFLKKALL*AT NIW*DKYIHS*MQTQQLVVLCIPVKTS*EAYFL GK*KKMEMDVSFV*VWSSAEGQSRLLVCS*PRE G*KINPTFKGI*GRLDSIATGL*LELNSCL*FIS MFRLFIR*NMPQ*THSCTYQEKKERHRWRTVKG GQMSLKKCSMSDAKWEKAENKGCGIMVLTLSKD LKLHVCKSNVTQKKMQMVFHWNYQVLRTCWLSHR W*RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN *IHCWA*VTEIGTPFVLLGGGFLVCALLGFFPS RLSLTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV *ILLGKELFSPLLQGSNFSTEVPIWQNYTRKQY CNDTIT*GTLNC*RSALPNKTKMSLGLVRAFTS NNLSVLKSE*PFLY*VQCFLLFSHAHVTLEKMFT KMVLLH*CAPHIYGLF*VTFYGLFRFSS*L*HT YPNFANY*FAK**YK*QTALNLLIIKAAKQTGG KYTALFFAVLTCLLCIMKITVVPPPFFP*IK*K* HLFYVA*VSNMF*PFRMFPSCEDPYFMHTCLP* ASRTRAGSCILLFQMTGACPRPGIDYKSSKCLS KDLRDEVGDEPPSPGS*RQYGVKLVLKEMSHFCS FEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLMKLSMSTVGVRKKRRKCL NRKESQVPL*TCLVSLLFAEIS*DCSRRTRNVPR LFGDNSYYRSNSSGSRKNIKGNCWQRDRSGLSS RKNRGDG*KSNKGESGKLEGSRNVNSEGENKKK RGV*RSWREGAKKKRRGDGQKKKRGELKENRSIS GDS*KRSSGTWKSFSSSCSRSRPCYWSADGGRW RSTGRQRGSRGSCNKNKHISCLYSMTIGGRTRS TRSSRHHRSRKGASQASMLPSPKPTTSLLTERER FL*EQHLAPLFCPVEIPHCRAVGSRIARQDRET PPAVLSPGFCGREWRSWCPDLAVAAPQGPATQD PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAWK MQ*KNLKIKRKFSDPSSLLAKWI*PHWPKSFEQ WKMYGHLTK*RTTPHPVRSRGRRMRRTTMWSRK GLTSPPQDQRTPEQRHL*I*AMVKRNL*KP*LSM MM*KVSRP*PHPRRAL*SSARLSPLVAHSRNTN LPPPLHLL*TPDYYRFLHLAEQQ*HLWWDFPVM G*DQKP*GKILPGKAQWSM*ILPTLGHRVTPRRF VNTRRGLTLRFCVLPYGE*IC*WVQRVA*CCWT EVAKGRSILLSTEDDFNKWTYLRA*MSW*QYLA KRISYVSTICPG*EIKYFTMIQKLRRSRDGQP*G IWKDVYIIKL*NMKESNFW*LL*RVLWKSMRGH QSHITNLWPLSHLENWYISHYWWISLLRKARG* K*SMDPVLDSMLLMWIQDQSMTFIYQHISSVASN PMQSSSSPIQMEWSFWCAMKMRGFM*THMEGSP RM*FYSGERCLHQ*HIFDPIRQWAGERRP*RSD LWKLVTWMVCSCTKGLKD*NSCVNAMTRCSLPLF GLVAAVRFIS*P*AGLLF*AGRSSVIQGLLASR VFKILRTWNSL*LELGAAPRATRTAVCADLMCW VLSPPSCSSYIPVYPHSFFFSYSKINQGCNAAGA VQILPSGAISVWD*ASYWKANTFPPAPEFLVSE *LCLVGV*QWRR*TCRWFYWQWAQGGEKWW*KE RSAEAESRFNIVTLTVYLIDISFL*CDDMWTKKK MQV*EVNIYKM*KTQLLG*LFCGWGLGDGVGWV KGSHFVSLDLGWGSWPRTQSFFCVPGCLNHVQM VLKKKKKKKKKGKKKRKRKRVHFV*WPELCRVTV LALPQLKV*FLFKPRRATKVLPQSALAEERKKF KPHVYFFFAYLIVLNALRGDLVYMFLEETINV* FNLNTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G *CSLLTSLETRMLCFYQIHH*RLMGQLQPMTQD LIVLDVEGLVADRWLH*NSHTNCSVLQEPFLGG GGVSLF*KCNALKLF*ECS*FCLFIKWASSVF*V *YRSPGFSRFLTCSLVLCFFKPILLYEYIHDIC NKCLEKEFVSWLHGHHSSSRKDITVSGKDQDSM SQSCHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF *A*SFSFVVNFPVYCENGGFSLCSHPPKHSSHL YLFASHPTWHPL*PLVSFLFLVPSF*KRPSFEL QTSGKTSTSTHECRHSSWWFCVYL*RQAEKLTFW GSKRRWKWMFHLYEFGAVLKAKAAYWFVVNLEK VEKLILPLKGFEVGWIPSPQDFS*N*IPACNLY PCLGFS*DETCHSEHTRVHIKRRRKGTGGEQ*KV GRCL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT *SSMSVSQMLHKKKCKWCFIGITKCLELAGFPI GGKGV*AHTELCLACLCSSRHPVGTLVVFVVN*I ESIVGLKLLKLEHPLSFSAGASWSVLYLAFFLP VLASPPCQPD*VAIA*CRDPVKFLR*RLGVVEM FRFF*ERNYFPPFYRVVTSPQKCQYGKITQENSI AMTPLHKEH*TVRGVLFQTKQKCL*V*SELSQV ITFLY*NQSNPFCIECSVFYSFLMHMLRWRKCL QKWFCYTNAHHIFMVYFK*LFMGYLGFRLSCSTL TLILPIINLLNSNTNDKLH*IY*L*KLQSRLVA STQPFFLQC*LVYCVL*KLLLSPHPFFLK*SKN DTYFMWHEFRICSDPSECFLPVRIHILCIPAYPE LPVPEQAPVFCYFR*QGLAQGQV*ITKVANVCL KTSEMKWEMNLHHQVPEDSME*SWSLKKCHIFA VLNLDQKI

Sequence MAP4K4.fAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCC CACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGT GCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGA GTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGC CTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGA GGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTG AATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAA TCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCC ATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTC AATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGG GAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACA AATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTT CACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAAT TTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACA TAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGA TCAGTCTATGACATTTATCTACCAACACATGTAAGAAAGAACCCACACTCTATGATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATA CAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGAT GCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTG TTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATT TCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATT CCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTA TATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATA AGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGT GGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAA TATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTAT AAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATT TGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAA AAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAA TTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCT ACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATT GAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAA ACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGG TAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAA TGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGT TTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTT CATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTT GTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTT AATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGC ACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCAC TCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATG TTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGG GATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAAC ATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAA TGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGT TACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGA GTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTG AAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAG ATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTT TCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACAT TGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTA ACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCA CCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTG CTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCT AACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCG AATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTA TTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGA ACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP CSRAQSPLRAC*PSARGSCENNISLPCSVPSRFP TAGQWAAE*PGRTEKLHQQY*AQASVGESGEAG AQTWQWQLLRVQQLRIPARVSPWVSEWLRGTLQ SEIIIQV*RLSISAPGKCSEKT*R*KGSFQTPQA CWRSGSDRTGQRASSSGRCTATSQSNGLLLIQ* GVGDDG*GGRRCGAGRG*RVHLRTRGHQSSVIS EFEQW*NGICENHDCP**CRK*AGHDPIQGGHSN RPPDSVR**HTPETQIFLLLYTFYRPQITTDFS I*RNNSDICGGIFL*WDETRSHKARSYPERLSG QCESYQH*ATE*HPGDS*IQEEV*L*DSVCCLMG SEFASGYREWPDAAGQKWPREGLSSYQPKTIST NGRT*GLECLGDNIWQKG*VTCLLFVLVKK*NT SQ*SRS*EEAGMDNRRGFGRMCTL*SCKI*KNQI SGDCFEEFCGSLCVGTKAISQIYGL*VIWRIGT *AITGGSHC*GRPEVESDLWILCWIPCC*CGFR ISL*HLSTNTCKKEPTLYDPV*HQTPCNHHPPQY RWNGASGVL*R*GGLCKHIWKDHQGCSSTVGRD AYISSIYSIQSDNGLGREGHRDPICGNWSLGWC VHAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGLF HDLRQDFSSELVEAV*SRDYWPPESSRS*ELGI PCNWSSELHRGQPGQLCVQTSCVGFSPLLPVPL IYQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL* VFGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDS GDDEHAVGFIGSGHKEVRSGGKRSGVLKQRADL I**H*QCI*LTFLFCNVTICGQRRRCRFKKLIFI KCERHSY*DNFFVGGAWEMGWGGLRGPILFLWI WGGGPGQELSHFSVYQVA*IMCRWF*KKKKKKK KKEKKKEKENVCILYNGQNFVV*QY*HCLS*RFN FCLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLP TSLFLMH*EVI*FICFWKKPLMFNLILIPKRPD *SLTFIVTNQQAQELSMKMGNSLKADAVYLQV*K PECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW *QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNA MH*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFLV FSLALLFSVFLNQFYFMNIFMTFVINVLRKNLF HGFMVITQAPVRILPSQERIRTPCHSPAILLSS CRVLSGNNCIMAALTSVIKRNLLFFRLDLFPLWL IFLYIVKMGDFPSAPTHLNTAAICTCLLPIPLG THSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHP LMNADTAAGGFVYTCKDKLRSLLFGEVKEDGNGC FICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*R DLR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMK HATVNTLVYISREEGKAQVENSKRWADVFEEMLN V*C*VGEGREQRMWHNGLNIIQRLEAPCL*VKC YTKKNANGVSLELPSA*NLLAFP*VVKGSELTP SCAWLACAAPGTRWALWWCLW*TELNPLLGLSY* NWNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQ IELL*LDAGTQ*SFSVKDWESSKCLDSFRKGII FPPFTG**LLHRSANMAKLHKKTVLQ*HHYIRNI ELLEECSSKQNKNVSRFSQSFHK**PFCIKIRV TLSVLSAVFFTLFSCTCYVGENVYKNGFVTLMR TTYLWFILSDFLWVI*VFVLVVAHLP*FCQLLIC *IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSA NLSTVYYENYCCPPTLFSLNKVKMTPILCGMSF EYVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLLY FAISDDRGLPKARYRLQK*QMFV*RPQR*SGR* TSITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH APEPKAHYEPADRAREVPVRTTSRSPVLSRRDS PLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLV PRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFR VRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLK PAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSE ESGTTDEEDDDVEQEGADESTSGPEDTRAASSL NLSNGETESVKTMIVHDDVESEPAMTPSKEGTL IVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISP SSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVV NVNPTNTRPQSDTPEIRKYKKRFNSEILCAALW GVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQ MDVLEGLNVLVTISGKKDKLRVYYLSWLRNKIL HNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIK FLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVH KPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSG SVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPN TDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEM PTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGV FMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY FMTLGRTSLLSW*KQCDPGITGLQSLQDPENLEF LVTGARSCTEGNQDSCVCRPHVLGSLPSFLFLL YTSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCY KCLGLSIILESKHLSSSSRIPCL*MTLSCGCLTV ATMNMPLVLLAVGTRR*EVVVKGAEC*SREQI* YSNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL *NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFGF GVGVLAKNSVIFLCTRLPKSCADGSKKKKKKKK KRKKKKKKKTCAFCIMARTLSCDSISTASVKGL IFV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFCL PHCS*CIER*FSLYVFGRNH*CLI*S*YQNDQI EV*LLLSQISRHKNCP*RWEIALRLMQFTYKFR NQNALFLPDSPLEVDGATAAHDTRSHCSRCRGVG SRQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQ CTKTILRM*LILLIHKVGIFCVLGVISKSWLFS FSHLLSCSLFF*TNFTL*IYS*HL**MS*ERICF MASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPL VEF*VEITALWLL*PQSSKETCCFLGLIFFLCG *FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHLA PTLTSCQFPVPGSIFLKKALL*ATNIW*DKYIH S*MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMD VSFV*VWSSAEGQSRLLVCS*PREG*KINPTFKG I*GRLDSIATGL*LELNSCL*FISMFRLFIR*N MPQ*THSCTYQEKKERHRWRTVKGGQMSLKKCS MSDAKWEKAENKGCGIMVLTLSKDLKLHVCKSNV TQKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHR VVLGLLVQLQAPGGHSGGVCGELN*IHCWA*VT EIGTPFVLLGGGFLVCALLGFFPSRLSLTPLSTR LSCYSLMQGPSEVSPLKIGSRRNV*ILLGKELF SPLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GT LNC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE* PFLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CA PHIYGLF*VTFYGLFRFSS*L*HTYPNFANY*F AK**YK*QTALNLLIIKAAKQTGGKYTALFFAVL TCLLCIMKITVVPPPFFP*IK*K*HLFYVA*VS NMF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSC ILLFQMTGACPRPGIDYKSSKCLSKDLRDEVGDE PPSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM LPSPKPTTSLLTERERFL*EQHLAPLFCPVEIP HCRAVGSRIARQDRETPPAVLSPGFCGREWRSW CPDLAVAAPQGPATQDPSPGLTLGLRVAPGNASE *DHHPSLKALHLSAWKMQ*KNLKIKRKFSDPSS LLAKWI*PHWPKSFEQWKMYGHLTK*RTTPHPV RSRGRRMRRTTMWSRKGLTSPPQDQRTPEQRHL* I*AMVKRNL*KP*LSMMM*KVSRP*PHPRRAL* SSARLSPLVAHSRNTNLPPPLHLL*TPDYYRFL HLAEQQ*HLWWDFPVMG*DQKP*GKILPGKAQWS M*ILPTLGHRVTPRRFVNTRRGLTLRFCVLPYG E*IC*WVQRVA*CCWTEVAKGRSILLSTEDDFN KWTYLRA*MSW*QYLAKRISYVSTICPG*EIKYF TMIQKLRRSRDGQP*GIWKDVYIIKL*NMKESN FW*LL*RVLWKSMRGHQSHITNLWPLSHLENWY ISHYWWISLLRKARG*K*SMDPVLDSMLLMWIQD QSMTFIYQHM*ERTHTL*SSVASNPMQSSSSPI QMEWSFWCAMKMRGFM*THMEGSPRM*FYSGER CLHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMVC SCTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFI S*P*AGLLF*AGRSSVIQGLLASRVFKILRTWN SL*LELGAAPRATRTAVCADLMCWVLSPPSCSSY IPVYPHSFFFSYSKINQGCNAAGAVQILPSGAI SVWD*ASYWKANTFPPAPEFLVSE*LCLVGV*Q WRR*TCRWFYWQWAQGGEKWW*KERSAEAESRFN IVTLTVYLIDISFL*CDDMWTKKKMQV*EVNIY KM*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLD LGWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKKK KGKKKRKRKRVHFV*WPELCRVTVLALPQLKV* FLFKPRRATKVLPQSALAEERKKFKPHVYFFFA YLIVLNALRGDLVYMFLEETINV*FNLNTKTTRL KFDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLE TRMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGL VADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KCN ALKLF*ECS*FCLFIKWASSVF*V*YRSPGFSR FLTCSLVLCFFKPILLYEYIHDICNKCLEKEFV SWLHGHHSSSRKDITVSGKDQDSMSQSCHLTFLL SSSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVV NFPVYCENGGFSLCSHPPKHSSHLYLFASHPTW HPL*PLVSFLFLVPSF*KRPSFELQTSGKTSTST HECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWM FHLYEFGAVLKAKAAYWFVVNLEKVEKLILPLK GFEVGWIPSPQDFS*N*IPACNLYPCLGFS*DET CHSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQ CLMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQM LHKKKCKWCFIGITKCLELAGFPIGGKGV*AHTE LCLACLCSSRHPVGTLVVFVVN*IESIVGLKLL KLEHPLSFSAGASWSVLYLAFFLPVLASPPCQP D*VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNYF PPFYRVVTSPQKCQYGKITQENSIAMTPLHKEH *TVRGVLFQTKQKCL*V*SELSQVITFLY*NQS NPFCIECSVFYSFLMHMLRWRKCLQKWFCYTNAH HIFMVYFK*LFMGYLGFRLSCSTLTLILPIINL LNSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC *LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEFR ICSDPSECFLPVRIHILCIPAYPELPVPEQAPV FCYFR*QGLAQGQV*ITKVANVCLKTSEMKWEM NLHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Sequence MAP4K4.cAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCC CACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCC CAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTG AGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTG CTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGA GTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAAT TTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCG TCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATC TAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAAT GTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAG TGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAAT GGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCAC AATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTC TGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAA GCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCA GTCTATGACATTTATCTACCAACACATGTAAGAAAGAACCCACACTCTATGATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAG ATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCC TACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTC ATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCA TGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCT TGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATAT ACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGT GTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGC GACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATAT AGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAA ATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGG GGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA AGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTT TTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACC TCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAA GTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACC AGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAG CAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGC ACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTT CTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCAT GGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTC GAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAAT TTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACC CACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCA TGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTT CATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGAT TTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATG CCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGT CTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTAC ACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTT GTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAA TTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATT GAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCC CCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACATTGA ACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACC CTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCA CATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTA AATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAAC TTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAAT ATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTT TGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACC TCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP CSRAQSPLRAC*PSARGSCENNISLPCSVPSRFP TAGQWAAE*PGRTEKLHQY*AQASVGESGEAGA QTWQWQLLRVQQLRIPARVSPWVSEWLRGTLQS EIIIQV*RLSISAPGKCSEKT*R*KGSFQTPQAC WRSGSDRTGQRASSSGRCTATSQSNGLLLIQ*G VGDDG*GGRRCGAGRG*RVHLRTRGHQSSVISE FEQW*NGICENHDCP**CRK*AGHDPIQGGHSNR PPDSVR**HTPETQIFLLLYTFYRPQITTDFSI *RNNSDICGGIFL*WDETRSHKARSYPERLSGQ CESYQH*ATE*HPGDS*IQEEV*L*DSVCCLMGS EFASGYREWPDAAGQKWPREGLSSYQPKTISTN GRT*GLECLGDNIWQKG*VTCLLFVLVKK*NTS Q*SRS*EEAGMDNRRGFGRMCTL*SCKI*KNQIS GDCFEEFCGSLCVGTKAISQIYGL*VIWRIGT* AITGGSHC*GRPEVESDLWILCWIPCC*CGFRI SL*HLSTNTCKKEPTLYDPV*HQTPCNHHPPQYR WNGASGVL*R*GGLCKHIWKDHQGCSSTVGRDA YISSIYSIQSDNGLGREGHRDPICGNWSLGWCV HAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGLFH DLRQDFSSELVEAV*SRDYWPPESSRS*ELGIP CNWSSELHRGQPGQLCVQTSCVGFSPLLPVPLI YQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL*V FGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDSG DDEHAVGFIGSGHKEVRSGGKRSGVLKQRADLI **H*QCI*LTFLFCNVTICGQRRRCRFKKLIFIK CERHSY*DNFFVGGAWEMGWGGLRGPILFLWIW GGGPGQELSHFSVYQVA*IMCRWF*KKKKKKKK KEKKKEKENVCILYNGQNFVV*QY*HCLS*RFNF CLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLPT SLFLMH*EVI*FICFWKKPLMFNLILIPKRPD* SLTFIVTNQQAQELSMKMGNSLKADAVYLQV*KP ECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW* QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNAM H*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFLVF SLALLFSVFLNQFYFMNIFMTFVINVLRKNLFH GFMVITQAPVRILPSQERIRTPCHSPAILLSSC RVLSGNNCIMAALTSVIKRNLLFFRLDLFPLWLI FLYIVKMGDFPSAPTHLNTAAICTCLLPIPLGT HSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHPL MNADTAAGGFVYTCKDKLRSLLFGEVKEDGNGCF ICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*RD LR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMKH ATVNTLVYISREEGKAQVENSKRWADVFEEMLNV *C*VGEGREQRMWHNGLNIIQRLEAPCL*VKCY TKKNANGVSLELPSA*NLLAFP*VVKGSELTPS CAWLACAAPGTRWALWWCLW*TELNPLLGLSY*N WNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQI ELL*LDAGTQ*SFSVKDWESSKCLDSFRKGIIF PPFTG**LLHRSANMAKLHKKTVLQ*HHYIRNIE LLEECSSKQNKNVSRFSQSFHK**PFCIKIRVT LSVLSAVFFTLFSCTCYVGENVYKNGFVTLMRT TYLWFILSDFLWVI*VFVLVVAHLP*FCQLLIC* IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSAN LSTVYYENYCCPPTLFSLNKVKMTPILCGMSFE YVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLLYF AISDDRGLPKARYRLQK*QMFV*RPQR*SGR*T SITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH APEPKAHYEPADRAREVPVRTTSRSPVLSRRDS PLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVP RPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRV RSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKP AGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEE SGTTDEEDDDVEQEGADESTSGPEDTRAASSLN LSNGETESVKTMIVHDDVESEPAMTPSKEGTLI VRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPS SGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVN VNPTNTRPQSDTPEIRKYKKRFNSEILCAALWG VNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQM DVLEGLNVLVTISGKKDKLRVYYLSWLRNKILH NDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKF LVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHK PLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGS VYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNT DGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMP TSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVF MHKRAQRLKFLCERNDKVFFASVRSGGSSQVYF MTLGRTSLLSW*KQCDPGITGLQSLQDPENLEFL VTGARSCTEGNQDSCVCRPHVLGSLPSFLFLLY TSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCYK CLGLSIILESKHLSSSSRIPCL*MTLSCGCLTVA TMNMPLVLLAVGTRR*EVVVKGAEC*SREQI*Y SNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL* NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFGFG VGVLAKNSVIFLCTRLPKSCADGSKKKKKKKKK RKKKKKKKTCAFCIMARTLSCDSISTASVKGLI FV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFCLP HCS*CIER*FSLYVFGRNH*CLI*S*YQNDQIE V*LLLSQISRHKNCP*RWEIALRLMQFTYKFRN QNALFLPDSPLEVDGATAAHDTRSHCSRCRGVGS RQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQC TKTILRM*LILLIHKVGIFCVLGVISKSWLFSF SHLLSCSLFF*TNFTL*IYS*HL**MS*ERICFM ASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPLV EF*VEITALWLL*PQSSKETCCFLGLIFFLCG* FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHLAP TLTSCQFPVPGSIFLKKALL*ATNIW*DKYIHS *MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMDV SFV*VWSSAEGQSRLLVCS*PREG*KINPTFKGI *GRLDSIATGL*LELNSCL*FISMFRLFIR*NM PQ*THSCTYQEKKERHRWRTVKGGQMSLKKCSM SDAKWEKAENKGCGIMVLTLSKDLKLHVCKSNVT QKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHRV VLGLLVQLQAPGGHSGGVCGELN*IHCWA*VTE IGTPFVLLGGGFLVCALLGFFPSRLSLTPLSTRL SCYSLMQGPSEVSPLKIGSRRNV*ILLGKELFS PLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GTL NC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE*P FLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CAP HIYGLF*VTFYGLFRFSS*L*HTYPNFANY*FA K**YK*QTALNLLIIKAAKQTGGKYTALFFAVLT CLLCIMKITVVPPPFFP*IK*K*HLFYVA*VSN MF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSCI LLFQMTGACPRPGIDYKSSKCLSKDLRDEVGDEP PSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM LPSPKPTTSLLTERERFL*EQHLAPLFCPVEIP HCRAVGSRIARQDRETPPVLSPGFCGREWRSWC PDLAVAAPQGPATQDPSPGLTLGLRVAPGNASE* DHHPSLKALHLSAWKMQ*KNLKIKRKFSDPSSL LAKWI*PHWPKSFEQWKMYGHLTK*RTTPHPVR SRGRRMRRTTMWSRKGLTSPPQDQRTPEQRHL*I *AMVKRNL*KP*LSMMM*KVSRP*PHPRRAL*S SARLSPLVAHSRNTNLPPPLHLL*TPDYYRFLH LAEQQ*HLWWDFPVMG*DQKP*GKILPGKAQWSM *ILPTLGHRVTPRRFVNTRRGLTLRFCVLPYGE *IC*WVQRVA*CCWTEVAKGRSILLSTEDDFNK WTYLRA*MSW*QYLAKRISYVSTICPG*EIKYFT MIQKLRRSRDGQP*GIWKDVYIIKL*NMKESNF W*LL*RVLWKSMRGHQSHITNLWPLSHLENWYI SHYWWISLLRKARG*K*SMDPVLDSMLLMWIQDQ SMTFIYQHM*ERTHTL*SSVASNPMQSSSSPIQ MEWSFWCAMKMRGFM*THMEGSPRM*FYSGERC LHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMVCS CTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFIS *P*AGLLF*AGRSSVIQGLLASRVFKILRTWNS L*LELGAAPRATRTAVCADLMCWVLSPPSCSSYI PVYPHSFFFSYSKINQGCNAAGAVQILPSGAIS VWD*ASYWKANTFPPAPEFLVSE*LCLVGV*QW RR*TCRWFYWQWAQGGEKWW*KERSAEAESRFNI VTLTVYLIDISFL*CDDMWTKKKMQV*EVNIYK M*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLDL GWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKKKK GKKKRKRKRVHFV*WPELCRVTVLALPQLKV*F LFKPRRATKVLPQSALAEERKKFKPHVYFFFAY LIVLNALRGDLVYMFLEETINV*FNLNTKTTRLK FDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLET RMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGLV ADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KCNA LKLF*ECS*FCLFIKWASSVF*V*YRSPGFSRF LTCSLVLCFFKPILLYEYIHDICNKCLEKEFVS WLHGHHSSSRKDITVSGKDQDSMSQSCHLTFLLS SSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVVN FPVYCENGGFSLCSHPPKHSSHLYLFASHPTWH PL*PLVSFLFLVPSF*KRPSFELQTSGKTSTSTH ECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWMF HLYEFGAVLKAKAAYWFVVNLEKVEKLILPLKG FEVGWIPSPQDFS*N*IPACNLYPCLGFS*DETC HSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQC LMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQML HKKKCKWCFIGITKCLELAGFPIGGKGV*AHTEL CLACLCSSRHPVGTLVVFVVN*IESIVGLKLLK LEHPLSFSAGASWSVLYLAFFLPVLASPPCQPD *VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNYFP PFYRVVTSPQKCQYGKITQENSIAMTPLHKEH* TVRGVLFQTKQKCL*V*SELSQVITFLY*NQSN PFCIECSVFYSFLMHMLRWRKCLQKWFCYTNAHH IFMVYFK*LFMGYLGFRLSCSTLTLILPIINLL NSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC* LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEFRI CSDPSECFLPVRIHILCIPAYPELPVPEQAPVF CYFR*QGLAQGQV*ITKVANVCLKTSEMKWEMN LHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Sequence MAP4K4.bAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTC CTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCG AGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGAC AGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCA CAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGTTTCCCCTGTCTCGCGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTTGCACACCACC ATCTTCGTTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGTGCTCAGTCAGAGCTCTGACTCTAAGTCAGAGGCGCCTGACCCTACCCAAAAGGCTTG GTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGT GGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCA GTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATC CAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTG GATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGA CGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGG TGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACT CAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAA CAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTAC CAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTA GTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTG AGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGA AGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCT TTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGG TGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACAT TTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAG GGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAA TGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTG TGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAG AAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACC AGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACT CCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACAC CTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGG GCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTT TTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTG TGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTC TGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATT TTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACC ACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTT TATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAA GAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAG GTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAAC TGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCT TATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACC AATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGAT ATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCT TTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCT CTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTT CCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTAT ACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAA AGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTT AGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAA GAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGA TGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATT ACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGG TGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTC CTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAG TTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGT GCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAA ATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTT CTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGG GTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTA CTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCC CCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGA GGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCA GGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAA AGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Multiple exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTGVPMAGDGGAPAGREAPEAVATRTSIS PVSTA*P*EAAPAALAAAATTAAGKEQAKLPCSR AQSPLRAC*PSARGGR*I*EN*PQLP*SPV*AD RQSIGATSAFPIRVFFQWQLRVCASRPAETSGA TGTVVPPGISQEQCFPCLAIPFLQ*PFSQICTPP SSFSGPMSTFPQ*GAQSEL*L*VRGA*PYPKGL V*IRQ*RGASKGSCENNISLPCSVPSRFPTAGQ WAAE*PGRTEKLHQQY*AQASVGESGEAGAQTWQ WQLLRVQQLRIPARVSPWVSEWLRGTLQSEIII QV*RLSISAPGKCSEKT*R*KGSFQTPQACWRS GSDRTGQRASSSGRCTATSQSNGLLLIQ*GVGDD G*GGRRCGAGRG*RVHLRTRGHQSSVISEFEQW *NGICENHDCP**CRK*AGHDPIQGGHSNRPPD SVR**HTPETQIFLLLYTFYRPQITTDFSI*RNN SDICGGIFL*WDETRSHKARSYPERLSGQCESY QH*ATE*HPGDS*IQEEV*L*DSVCCLMGSEFA SGYREWPDAAGQKWPREGLSSYQPKTISTNGRT* GLECLGDNIWQKG*VTCLLFVLVKK*NTSQ*SR S*EEAGMDNRRGFGRMCTL*SCKI*KNQISGDC FEEFCGSLCVGTKAISQIYGL*VIWRIGT*AITG GSHC*GRPEVESDLWILCWIPCC*CGFRISL*H LSTNTYPV*HQTPCNHHPPQYRWNGASGVL*R* GGLCKHIWKDHQGCSSTVGRDAYISSIYSIQSDN GLGREGHRDPICGNWSLGWCVHAQKGSKTKILV *TQ*QGVLCLCSVWWQQSGLFHDLRQDFSSELV EAV*SRDYWPPESSRS*ELGIPCNWSSELHRGQP GQLCVQTSCVGFSPLLPVPLIYQFIPILFFFLT PK*IKAAMQLVLFRFYHQVL*VFGIEHHTGKQT PFLQLQNSLSLNDSVLWVSDSGDDEHAVGFIGSG HKEVRSGGKRSGVLKQRADLI**H*QCI*LTFL FCNVTICGQRRRCRFKKLIFIKCERHSY*DNFF VGGAWEMGWGGLRGPILFLWIWGGGPGQELSHFS VYQVA*IMCRWF*KKKKKKKKKEKKKEKENVCI LYNGQNFVV*QY*HCLS*RFNFCLNLDVQQKFY HSLHLQKKEKNSNHMFIFFLPTSLFLMH*EVI*F ICFWKKPLMFNLILIPKRPD*SLTFIVTNQQAQ ELSMKMGNSLKADAVYLQV*KPECFVFTRFTIR G*WGNCSP*HKISLFSM*RGW*QTGGYIRIVTQT VQCCRNLFLGVGEFPFSKNAMH*NYFKNVVNSA YS*SGHLLCFRCNIEVLAFLVFSLALLFSVFLN QFYFMNIFMTFVINVLRKNLFHGFMVITQAPVRI LPSQERIRTPCHSPAILLSSCRVLSGNNCIMAA LTSVIKRNLLFFRLDLFPLWLIFLYIVKMGDFP SAPTHLNTAAICTCLLPIPLGTHSDLLSVSCSWF HLFEKGPPLSYKHLVRQVHPLMNADTAAGGFVY TCKDKLRSLLFGEVKEDGNGCFICMSLEQC*RP KPPTGL*LT*RRLKN*SYL*RDLR*AGFHRHRTL VRIKFLLVIYIHV*AFHKMKHATVNTLVYISRE EGKAQVENSKRWADVFEEMLNV*C*VGEGREQR MWHNGLNIIQRLEAPCL*VKCYTKKNANGVSLEL PSA*NLLAFP*VVKGSELTPSCAWLACAAPGTR WALWWCLW*TELNPLLGLSY*NWNTLCPSRRGL PGLCFTWLFSFPS*PHPLVNQIELL*LDAGTQ*S FSVKDWESSKCLDSFRKGIIFPPFTG**LLHRS ANMAKLHKKTVLQ*HHYIRNIELLEECSSKQNK NVSRFSQSFHK**PFCIKIRVTLSVLSAVFFTLF SCTCYVGENVYKNGFVTLMRTTYLWFILSDFLW VI*VFVLVVAHLP*FCQLLIC*IVIQMTNCIKF TNYKSCKADWWQVHSPFFCSANLSTVYYENYCCP PTLFSLNKVKMTPILCGMSFEYVLTLQNVSFL* GSIFYAYLPTLSFPYQSRLLYFAISDDRGLPKA RYRLQK*QMFV*RPQR*SGR*TSITRFLKTVWSK AGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLECRWREMEEHRQAERLQRQLQQEQAYL LSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAP EPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQT GRVLEPPVPSRSESFSNGNSESVHPALQRPAEP QVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHH HLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAW SRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGS GQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPG SGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSS KSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEV DLTALAKELRAVEDVRPPHKVTDYSSSSEESGT TDEEDDDVEQEGADESTSGPEDTRAASSLNLSNG ETESVKTMIVHDDVESEPAMTPSKEGTLIVRQT QSASSTLQKHKSSSSFTPFIDPRLLQISPSSGT TVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPT NTRPQSDTPEIRKYKKRFNSEILCAALWGVNLL VGTESGLMLLDRSGQGKVYPLINRRRFQQMDVL EGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPE VEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIA LKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLL VDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDI YLPTHIQCSIKPHAIIILPNTDGMELLVCYEDE GVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQT MGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLC ERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW* KQCDPGITGLQSLQDPENLEFLVTGARSCTEGN QDSCVCRPHVLGSLPSFLFLLYTSLSPFFFFFLL QNKSRLQCSWCCSDSTIRCYKCLGLSIILESKH LSSSSRIPCL*MTLSCGCLTVATMNMPLVLLAV GTRR*EVVVKGAEC*SREQI*YSNINSVFN*HFF FVM*RYVDKEEDAGLRS*YL*NVKDTVTRITFL WVGLGRWGGVG*GVPFCFFGFGVGVLAKNSVIF LCTRLPKSCADGSKKKKKKKKKRKKKKKKKTCAF CIMARTLSCDSISTASVKGLIFV*T*TCNKSFT TVCTCRRKKKIQTTCLFFFCLPHCS*CIER*FS LYVFGRNH*CLI*S*YQNDQIEV*LLLSQISRHK NCP*RWEIALRLMQFTYKFRNQNALFLPDSPLE VDGATAAHDTRSHCSRCRGVGSRQVVTLE*SHK LFSVAGTFSWGWGSFPFLKMQCTKTILRM*LILL IHKVGIFCVLGVISKSWLFSFSHLLSCSLFF*T NFTL*IYS*HL**MS*ERICFMASWSSLKLP*G YYRLRKGSGLHVTVLPSYFPLVEF*VEITALWLL *PQSSKETCCFLGLIFFLCG*FSCIL*KWGIFP LLPPT*TQQPFVPVCFPSHLAPTLTSCQFPVPG SIFLKKALL*ATNIW*DKYIHS*MQTQQLVVLCI PVKTS*EAYFLGK*KKMEMDVSFV*VWSSAEGQ SRLLVCS*PREG*KINPTFKGI*GRLDSIATGL *LELNSCL*FISMFRLFIR*NMPQ*THSCTYQEK KERHRWRTVKGGQMSLKKCSMSDAKWEKAENKG CGIMVLTLSKDLKLHVCKSNVTQKKMQMVFHWN YQVLRTCWLSHRW*RGLSSHRVVLGLLVQLQAPG GHSGGVCGELN*IHCWA*VTEIGTPFVLLGGGF LVCALLGFFPSRLSLTPLSTRLSCYSLMQGPSE VSPLKIGSRRNV*ILLGKELFSPLLQGSNFSTEV PIWQNYTRKQYCNDTIT*GTLNC*RSALPNKTK MSLGLVRAFTSNNLSVLKSE*PFLY*VQCFLLF SHAHVTLEKMFTKMVLLH*CAPHIYGLF*VTFYG LFRFSS*L*HTYPNFANY*FAK**YK*QTALNL LIIKAAKQTGGKYTALFFAVLTCLLCIMKITVV PPPFFP*IK*K*HLFYVA*VSNMF*PFRMFPSCE DPYFMHTCLP*ASRTRAGSCILLFQMTGACPRP GIDYKSSKCLSKDLRDEVGDEPPSPGS*RQYGV KLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYWSADGGRWRSTGRQRGSRGSCNKNKHIS CLYSMTIGGRTRSTRSSRHHRSRKGASQASMLP SPKPTTSLLTERERWKIDLGKLTTAPLKPSLSR QAEYWSHQCLPDQSLFPMATPSLCIPPCRDQRSH RYSGPTWHLSRTMFPLSRDPIPSVTLLPNLHTT IFVLRTHVHLPAVRCSVRALTLSQRRLTLPKRL GLDQTVTRCLQGFL*EQHLAPLFCPVEIPHCRAV GSRIARQDRETPPAVLSPGFCGREWRSWCPDLA VAAPQGPATQDPSPGLTLGLRVAPGNASE*DHH PSLKALHLSAWKMQ*KNLKIKRKFSDPSSLLAKW I*PHWPKSFEQWKMYGHLTK*RTTPHPVRSRGR RMRRTTMWSRKGLTSPPQDQRTPEQRHL*I*AM VKRNL*KP*LSMMM*KVSRP*PHPRRAL*SSARL SPLVAHSRNTNLPPPLHLL*TPDYYRFLHLAEQ Q*HLWWDFPVMG*DQKP*GKILPGKAQWSM*IL PTLGHRVTPRRFVNTRRGLTLRFCVLPYGE*IC* WVQRVA*CCWTEVAKGRSILLSTEDDFNKWTYL RA*MSW*QYLAKRISYVSTICPG*EIKYFTMIQ KLRRSRDGQP*GIWKDVYIIKL*NMKESNFW*LL *RVLWKSMRGHQSHITNLWPLSHLENWYISHYW WISLLRKARG*K*SMDPVLDSMLLMWIQDQSMT FIYQHISSVASNPMQSSSSPIQMEWSFWCAMKMR GFM*THMEGSPRM*FYSGERCLHQ*HIFDPIRQ WAGERRP*RSDLWKLVTWMVCSCTKGLKD*NSC VNAMTRCSLPLFGLVAAVRFIS*P*AGLLF*AGR SSVIQGLLASRVFKILRTWNSL*LELGAAPRAT RTAVCADLMCWVLSPPSCSSYIPVYPHSFFFSY SKINQGCNAAGAVQILPSGAISVWD*ASYWKANT FPPAPEFLVSE*LCLVGV*QWRR*TCRWFYWQW AQGGEKWW*KERSAEAESRFNIVTLTVYLIDIS FL*CDDMWTKKKMQV*EVNIYKM*KTQLLG*LFC GWGLGDGVGWVKGSHFVSLDLGWGSWPRTQSFF CVPGCLNHVQMVLKKKKKKKKKGKKKRKRKRVH FV*WPELCRVTVLALPQLKV*FLFKPRRATKVLP QSALAEERKKFKPHVYFFFAYLIVLNALRGDLV YMFLEETINV*FNLNTKTTRLKFDFYCHKSAGT RTVHEDGK*P*G*CSLLTSLETRMLCFYQIHH*R LMGQLQPMTQDLIVLDVEGLVADRWLH*NSHTN CSVLQEPFLGGGGVSLF*KCNALKLF*ECS*FC LFIKWASSVF*V*YRSPGFSRFLTCSLVLCFFKP ILLYEYIHDICNKCLEKEFVSWLHGHHSSSRKD ITVSGKDQDSMSQSCHLTFLLSSSEWK*LHYGC FNLSHQKKLAVF*A*SFSFVVNFPVYCENGGFSL CSHPPKHSSHLYLFASHPTWHPL*PLVSFLFLV PSF*KRPSFELQTSGKTSTSTHECRHSSWWFCV YL*RQAEKLTFWGSKRRWKWMFHLYEFGAVLKAK AAYWFVVNLEKVEKLILPLKGFEVGWIPSPQDF S*N*IPACNLYPCLGFS*DETCHSEHTRVHIKR RRKGTGGEQ*KVGRCL*RNAQCLMLSGRRQRTKD VA*WS*HYPKT*SSMSVSQMLHKKKCKWCFIGI TKCLELAGFPIGGKGV*AHTELCLACLCSSRHP VGTLVVFVVN*IESIVGLKLLKLEHPLSFSAGAS WSVLYLAFFLPVLASPPCQPD*VAIA*CRDPVK FLR*RLGVVEMFRFF*ERNYFPPFYRVVTSPQK CQYGKITQENSIAMTPLHKEH*TVRGVLFQTKQK CL*V*SELSQVITFLY*NQSNPFCIECSVFYSF LMHMLRWRKCLQKWFCYTNAHHIFMVYFK*LFM GYLGFRLSCSTLTLILPIINLLNSNTNDKLH*IY *L*KLQSRLVASTQPFFLQC*LVYCVL*KLLLS PHPFFLK*SKNDTYFMWHEFRICSDPSECFLPV RIHILCIPAYPELPVPEQAPVFCYFR*QGLAQGQ V*ITKVANVCLKTSEMKWEMNLHHQVPEDSME* SWSLKKCHIFAVLNLDQKI