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Sequences

Sequence MAP4K4.lAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACC ACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGG AAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACT CCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACT GCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGA CCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGAT CATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGA TCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Single exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS GTWKSFSSSCSRSRPCYCMTIGGRTRSTRSSRH HRSRKGASQASMLPSPKPTTSLLTERERWKIDLG KLTTAPLKPSLSRQAEYWSHQCLPDQSLFPMAT PSLCIPPCRDQRSHRFL*EQHLAPLFCPVEIPH CRAVGSRIARQDRETPPVLSPGFCGREWRSWCPD LAVAAPQGPATQDPSPGLTLGLRVAPGNASE*D HHPSLKALHLSAWKMQ*KNLKIKRKFSDPSSLL I*PHWPKSFEQW

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY VATKERAGS*KAAGT*TAKERTRRKEASRGVGE KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA ALGSPSAAAAPGAGHVTA*P*EAAPAALAAAAT TAAGKEQAKLPCSRAQSPLRAC*PSARGGR*I* EN*PQLP*SPV*ADRQSIGATSAFPIRVFFQWQL RVCASRPAETSGATGSCENNISLPCSVPSRFPT AGQWAAE*PGRTEKLHQY*AQASVGESGEAGAQ TWQWQLLRVQQLRIPARVSPWVSEWLRGTLQSEI IIQV*RLSISAPGKCSEKT*R*KGSFQTPQAC* SDRTGQRASSSG

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT *QQRREREARRQQEREQRRREQEEKRRLEELER RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ RHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPP PQQERSKPSFHAPEPKAHYEPADRAREVEDRFR KTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGN SESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPL QGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPR PGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVR SSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAD LTALAKELRAV

Sequence MAP4K4.eAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAG CTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGG AGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCAT GATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAG AAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTT CCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACAC CCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATG CTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAA TATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGAC AACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTAT GCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCC AGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTG TAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGA AGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAG AGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTT TGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACT GGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTC ATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATG CAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCT TGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGG TAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGA CAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGT GGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCAT GTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTC GTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAG AAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATT AATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAAT AGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCAT GACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTT CTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTG TGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCA TGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAG GACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAAC TTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAG CAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCC TTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGC TTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAA CCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGT AATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAAC AGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTA TCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGC TTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGG TGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTT TTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTC GTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAG AAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCT TTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGA AAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTT GTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAG ACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAA AGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTG CCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAA TGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCAC ATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV* ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS APGKCSEKT*R*KGSFQTPQACWRSGSDRTGQR ASSSGRCTATSQSNGLLLIQ*GVGDDG*GGRRCG AGRG*RVHLRTRGHQSSVISEFEQW*NGICENH DCP**CRK*AGHDPIQGGHSNRPPDSVR**HTP ETQIFLLLYTFYRPQITTDFSI*RNNSDICGGIF L*WDETRSHKARSYPERLSGQCESYQH*ATE*H PGDS*IQEEV*L*DSVCCLMGSEFASGYREWPD AAGQKWPREGLSSYQPKTISTNGRT*GLECLGDN IWQKG*VTCLLFVLVKK*NTSQ*SRS*EEAGMD NRRGFGRMCTL*SCKI*KNQISGDCFEEFCGSL CVGTKAISQIYGL*VIWRIGT*AITGGSHC*GRP EVESDLWILCWIPCC*CGFRISL*HLSTNTYPV *HQTPCNHHPPQYRWNGASGVL*R*GGLCKHIW KDHQGCSSTVGRDAYISSIYSIQSDNGLGREGHR DPICGNWSLGWCVHAQKGSKTKILV*TQ*QGVL CLCSVWWQQSGLFHDLRQDFSSELVEAV*SRDY WPPESSRS*ELGIPCNWSSELHRGQPGQLCVQTS CVGFSPLLPVPLIYQFIPILFFFLTPK*IKAAM QLVLFRFYHQVL*VFGIEHHTGKQTPFLQLQNS LSLNDSVLWVSDSGDDEHAVGFIGSGHKEVRSGG KRSGVLKQRADLI**H*QCI*LTFLFCNVTICG QRRRCRFKKLIFIKCERHSY*DNFFVGGAWEMG WGGLRGPILFLWIWGGGPGQELSHFSVYQVA*IM CRWF*KKKKKKKKKEKKKEKENVCILYNGQNFV V*QY*HCLS*RFNFCLNLDVQQKFYHSLHLQKK EKNSNHMFIFFLPTSLFLMH*EVI*FICFWKKPL MFNLILIPKRPD*SLTFIVTNQQAQELSMKMGN SLKADAVYLQV*KPECFVFTRFTIRG*WGNCSP *HKISLFSM*RGW*QTGGYIRIVTQTVQCCRNLF LGVGEFPFSKNAMH*NYFKNVVNSAYS*SGHLL CFRCNIEVLAFLVFSLALLFSVFLNQFYFMNIF MTFVINVLRKNLFHGFMVITQAPVRILPSQERIR TPCHSPAILLSSCRVLSGNNCIMAALTSVIKRN LLFFRLDLFPLWLIFLYIVKMGDFPSAPTHLNT AAICTCLLPIPLGTHSDLLSVSCSWFHLFEKGPP LSYKHLVRQVHPLMNADTAAGGFVYTCKDKLRS LLFGEVKEDGNGCFICMSLEQC*RPKPPTGL*L T*RRLKN*SYL*RDLR*AGFHRHRTLVRIKFLLV IYIHV*AFHKMKHATVNTLVYISREEGKAQVEN SKRWADVFEEMLNV*C*VGEGREQRMWHNGLNI IQRLEAPCL*VKCYTKKNANGVSLELPSA*NLLA FP*VVKGSELTPSCAWLACAAPGTRWALWWCLW *TELNPLLGLSY*NWNTLCPSRRGLPGLCFTWL FSFPS*PHPLVNQIELL*LDAGTQ*SFSVKDWES SKCLDSFRKGIIFPPFTG**LLHRSANMAKLHK KTVLQ*HHYIRNIELLEECSSKQNKNVSRFSQS FHK**PFCIKIRVTLSVLSAVFFTLFSCTCYVGE NVYKNGFVTLMRTTYLWFILSDFLWVI*VFVLV VAHLP*FCQLLIC*IVIQMTNCIKFTNYKSCKA DWWQVHSPFFCSANLSTVYYENYCCPPTLFSLNK VKMTPILCGMSFEYVLTLQNVSFL*GSIFYAYL PTLSFPYQSRLLYFAISDDRGLPKARYRLQK*Q MFV*RPQR*SGR*TSITRFLKTVWSKAGP*RNVT FLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ RLENAVKKPEDKKEVFRPLKPAGEVDLTALAKE LRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDV EQEGADESTSGPEDTRAASSLNLSNGETESVKTM IVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQ KHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGF SCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDT PEIRKYKKRFNSEILCAALWGVNLLVGTESGLM LLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVT ISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWT TVGDLEGCVHYKVVKYERIKFLVIALKSSVEVY AWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEG QRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQC SIKPHAIIILPNTDGMELLVCYEDEGVYVNTYG RITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAI EIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFF ASVRSGGSSQVYFMTLGRTSLLSW*KQCDPGIT GLQSLQDPENLEFLVTGARSCTEGNQDSCVCRP HVLGSLPSFLFLLYTSLSPFFFFFLLQNKSRLQC SWCCSDSTIRCYKCLGLSIILESKHLSSSSRIP CL*MTLSCGCLTVATMNMPLVLLAVGTRR*EVV VKGAEC*SREQI*YSNINSVFN*HFFFVM*RYVD KEEDAGLRS*YL*NVKDTVTRITFLWVGLGRWG GVG*GVPFCFFGFGVGVLAKNSVIFLCTRLPKS CADGSKKKKKKKKKRKKKKKKKTCAFCIMARTLS CDSISTASVKGLIFV*T*TCNKSFTTVCTCRRK KKIQTTCLFFFCLPHCS*CIER*FSLYVFGRNH *CLI*S*YQNDQIEV*LLLSQISRHKNCP*RWEI ALRLMQFTYKFRNQNALFLPDSPLEVDGATAAH DTRSHCSRCRGVGSRQVVTLE*SHKLFSVAGTF SWGWGSFPFLKMQCTKTILRM*LILLIHKVGIFC VLGVISKSWLFSFSHLLSCSLFF*TNFTL*IYS *HL**MS*ERICFMASWSSLKLP*GYYRLRKGS GLHVTVLPSYFPLVEF*VEITALWLL*PQSSKET CCFLGLIFFLCG*FSCIL*KWGIFPLLPPT*TQ QPFVPVCFPSHLAPTLTSCQFPVPGSIFLKKAL L*ATNIW*DKYIHS*MQTQQLVVLCIPVKTS*EA YFLGK*KKMEMDVSFV*VWSSAEGQSRLLVCS* PREG*KINPTFKGI*GRLDSIATGL*LELNSCL *FISMFRLFIR*NMPQ*THSCTYQEKKERHRWRT VKGGQMSLKKCSMSDAKWEKAENKGCGIMVLTL SKDLKLHVCKSNVTQKKMQMVFHWNYQVLRTCW LSHRW*RGLSSHRVVLGLLVQLQAPGGHSGGVCG ELN*IHCWA*VTEIGTPFVLLGGGFLVCALLGF FPSRLSLTPLSTRLSCYSLMQGPSEVSPLKIGS RRNV*ILLGKELFSPLLQGSNFSTEVPIWQNYTR KQYCNDTIT*GTLNC*RSALPNKTKMSLGLVRA FTSNNLSVLKSE*PFLY*VQCFLLFSHAHVTLE KMFTKMVLLH*CAPHIYGLF*VTFYGLFRFSS*L *HTYPNFANY*FAK**YK*QTALNLLIIKAAKQ TGGKYTALFFAVLTCLLCIMKITVVPPPFFP*I K*K*HLFYVA*VSNMF*PFRMFPSCEDPYFMHTC LP*ASRTRAGSCILLFQMTGACPRPGIDYKSSK CLSKDLRDEVGDEPPSPGS*RQYGVKLVLKEMS HFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL SAWKMQ*KNLKIKRKFSDPSSLLAKWI*PHWPKS FEQWKMYGHLTK*RTTPHPVRSRGRRMRRTTMW SRKGLTSPPQDQRTPEQRHL*I*AMVKRNL*KP *LSMMM*KVSRP*PHPRRAL*SSARLSPLVAHSR NTNLPPPLHLL*TPDYYRFLHLAEQQ*HLWWDF PVMG*DQKP*GKILPGKAQWSM*ILPTLGHRVT PRRFVNTRRGLTLRFCVLPYGE*IC*WVQRVA*C CWTEVAKGRSILLSTEDDFNKWTYLRA*MSW*Q YLAKRISYVSTICPG*EIKYFTMIQKLRRSRDG QP*GIWKDVYIIKL*NMKESNFW*LL*RVLWKSM RGHQSHITNLWPLSHLENWYISHYWWISLLRKA RG*K*SMDPVLDSMLLMWIQDQSMTFIYQHISS VASNPMQSSSSPIQMEWSFWCAMKMRGFM*THME GSPRM*FYSGERCLHQ*HIFDPIRQWAGERRP* RSDLWKLVTWMVCSCTKGLKD*NSCVNAMTRCS LPLFGLVAAVRFIS*P*AGLLF*AGRSSVIQGLL ASRVFKILRTWNSL*LELGAAPRATRTAVCADL MCWVLSPPSCSSYIPVYPHSFFFSYSKINQGCN AAGAVQILPSGAISVWD*ASYWKANTFPPAPEFL VSE*LCLVGV*QWRR*TCRWFYWQWAQGGEKWW *KERSAEAESRFNIVTLTVYLIDISFL*CDDMW TKKKMQV*EVNIYKM*KTQLLG*LFCGWGLGDGV GWVKGSHFVSLDLGWGSWPRTQSFFCVPGCLNH VQMVLKKKKKKKKKGKKKRKRKRVHFV*WPELC RVTVLALPQLKV*FLFKPRRATKVLPQSALAEER KKFKPHVYFFFAYLIVLNALRGDLVYMFLEETI NV*FNLNTKTTRLKFDFYCHKSAGTRTVHEDGK *P*G*CSLLTSLETRMLCFYQIHH*RLMGQLQPM TQDLIVLDVEGLVADRWLH*NSHTNCSVLQEPF LGGGGVSLF*KCNALKLF*ECS*FCLFIKWASS VF*V*YRSPGFSRFLTCSLVLCFFKPILLYEYIH DICNKCLEKEFVSWLHGHHSSSRKDITVSGKDQ DSMSQSCHLTFLLSSSEWK*LHYGCFNLSHQKK LAVF*A*SFSFVVNFPVYCENGGFSLCSHPPKHS SHLYLFASHPTWHPL*PLVSFLFLVPSF*KRPS FELQTSGKTSTSTHECRHSSWWFCVYL*RQAEK LTFWGSKRRWKWMFHLYEFGAVLKAKAAYWFVVN LEKVEKLILPLKGFEVGWIPSPQDFS*N*IPAC NLYPCLGFS*DETCHSEHTRVHIKRRRKGTGGE Q*KVGRCL*RNAQCLMLSGRRQRTKDVA*WS*HY PKT*SSMSVSQMLHKKKCKWCFIGITKCLELAG FPIGGKGV*AHTELCLACLCSSRHPVGTLVVFV VN*IESIVGLKLLKLEHPLSFSAGASWSVLYLAF FLPVLASPPCQPD*VAIA*CRDPVKFLR*RLGV VEMFRFF*ERNYFPPFYRVVTSPQKCQYGKITQ ENSIAMTPLHKEH*TVRGVLFQTKQKCL*V*SEL SQVITFLY*NQSNPFCIECSVFYSFLMHMLRWR KCLQKWFCYTNAHHIFMVYFK*LFMGYLGFRLS CSTLTLILPIINLLNSNTNDKLH*IY*L*KLQSR LVASTQPFFLQC*LVYCVL*KLLLSPHPFFLK* SKNDTYFMWHEFRICSDPSECFLPVRIHILCIP AYPELPVPEQAPVFCYFR*QGLAQGQV*ITKVAN VCLKTSEMKWEMNLHHQVPEDSME*SWSLKKCH IFAVLNLDQKI

Sequence MAP4K4.dAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTC CTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCG AGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGAC AGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCA CAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAA ACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATC CCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAA AATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATG TACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGA GTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTA GAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCT CCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACC AGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATAC AAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCC AAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAA GTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAA GGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCAT ATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTA TGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCA ATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAG TTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAAC TGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGT GGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAG ATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCC CTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGAT TCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGT CTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTG AAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGT TTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCC CATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAA AAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACT GCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACAT GTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTA ATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAG TTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTT CTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTT CCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCG AAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGT CTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCT GCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTG ATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTT GCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTG GTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAA AAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAA TTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAG GCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATG TCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCC ATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAG GGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCAT TGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCC TCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCT TTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGAC ACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTT CTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGT TTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAAT TTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAG CCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATT TTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGT ACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCA GAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATT TGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTGVPMAGDGGAPAGREAPEAVATRTSIS PVSTA*P*EAAPAALAAAATTAAGKEQAKLPCSR AQSPLRAC*PSARGGR*I*EN*PQLP*SPV*AD RQSIGATSAFPIRVFFQWQLRVCASRPAETSGA TGSCENNISLPCSVPSRFPTAGQWAAE*PGRTEK LHQY*AQASVGESGEAGAQTWQWQLLRVQQLRI PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG KCSEKT*R*KGSFQTPQAC*SDRTGQRASSSGRC TATSQSNGLLLIQ*GVGDDG*GGRRCGAGRG*R VHLRTRGHQSSVISEFEQW*NGICENHDCP**C RK*AGHDPIQGGHSNRPPDSVR**HTPETQIFLL LYTFYRPQITTDFSI*RNNSDICGGIFL*WDET RSHKARSYPERLSGQCESYQH*ATE*HPGDS*I QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKWP REGLSSYQPKTISTNGRT*GLECLGDNIWQKG* VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKAI SQIYGL*VIWRIGT*AITGGSHC*GRPEVESDL WILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGCS STVGRDAYISSIYSIQSDNGLGREGHRDPICGN WSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESSR S*ELGIPCNWSSELHRGQPGQLCVQTSCVGFSP LLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDSV LWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGVL KQRADLI**H*QCI*LTFLFCNVTICGQRRRCR FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRGP ILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*K KKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNHM FIFFLPTSLFLMH*EVI*FICFWKKPLMFNLIL IPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISLF SM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGEF PFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVINV LRKNLFHGFMVITQAPVRILPSQERIRTPCHSP AILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTCL LPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKHL VRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLKN *SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV* AFHKMKHATVNTLVYISREEGKAQVENSKRWAD VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEAP CL*VKCYTKKNANGVSLELPSA*NLLAFP*VVK GSELTPSCAWLACAAPGTRWALWWCLW*TELNP LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*P HPLVNQIELL*LDAGTQ*SFSVKDWESSKCLDS FRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ* HHYIRNIELLEECSSKQNKNVSRFSQSFHK**PF CIKIRVTLSVLSAVFFTLFSCTCYVGENVYKNG FVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP* FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVHS PFFCSANLSTVYYENYCCPPTLFSLNKVKMTPI LCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RPQ R*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*I WIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLECRWREMEEHRQAERLQRQLQQEQAYL LSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAP EPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQT GRVLEPPVPSRSESFSNGNSESVHPALQRPAEP QVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQR NSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGS QPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE NAVKKPEDKKEVFRPLKPADLTALAKELRAVED VRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADE STSGPEDTRAASSLNLSNGETESVKTMIVHDDV ESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSS SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRP EAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKY KKRFNSEILCAALWGVNLLVGTESGLMLLDRSG QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDK LRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIY GSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHA IIILPNTDGMELLVCYEDEGVYVNTYGRITKDV VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVET GHLDGVFMHKRAQRLKFLCERNDKVFFASVRSG GSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSLP SFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCSD STIRCYKCLGLSIILESKHLSSSSRIPCL*MTL SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC* SREQI*YSNINSVFN*HFFFVM*RYVDKEEDAG LRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSKK KKKKKKKRKKKKKKKTCAFCIMARTLSCDSIST ASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S* YQNDQIEV*LLLSQISRHKNCP*RWEIALRLMQ FTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGSF PFLKMQCTKTILRM*LILLIHKVGIFCVLGVIS KSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTVL PSYFPLVEF*VEITALWLL*PQSSKETCCFLGL IFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNIW *DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK* KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K INPTFKGI*GRLDSIATGL*LELNSCL*FISMFR LFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQM SLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*R GLSSHRVVLGLLVQLQAPGGHSGGVCGELN*IH CWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*IL LGKELFSPLLQGSNFSTEVPIWQNYTRKQYCND TIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKMV LLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYPN FANY*FAK**YK*QTALNLLIIKAAKQTGGKYT ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HLF YVA*VSNMF*PFRMFPSCEDPYFMHTCLP*ASR TRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFEF GSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYWSADGGRWRSTGRQRGSRGSCNKNKHIS CLYSMTIGGRTRSTRSSRHHRSRKGASQASMLP SPKPTTSLLTERERWKIDLGKLTTAPLKPSLSR QAEYWSHQCLPDQSLFPMATPSLCIPPCRDQRSH RFL*EQHLAPLFCPVEIPHCRAVGSRIARQDRE TPPVLSPGFCGREWRSWCPDLAVAAPQGPATQD PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAWK MQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQWKM YGHLTK*RTTPHPVRSRGRRMRRTTMWSRKGLT SPPQDQRTPEQRHL*I*AMVKRNL*KP*LSMMM* KVSRP*PHPRRAL*SSARLSPLVAHSRNTNLPP PLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVNT RRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEVA KGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIWK DVYIIKL*NMKESNFW*LL*RVLWKSMRGHQSH ITNLWPLSHLENWYISHYWWISLLRKARG*K*S MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPMQ SSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM* FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGLV AAVRFIS*P*AGLLF*AGRSSVIQGLLASRVFK ILRTWNSL*LELGAAPRATRTAVCADLMCWVLS PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQI LPSGAISVWD*ASYWKANTFPPAPEFLVSE*LC LVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQV *EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKGS HFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLAL PQLKV*FLFKPRRATKVLPQSALAEERKKFKPH VYFFFAYLIVLNALRGDLVYMFLEETINV*FNL NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*CS LLTSLETRMLCFYQIHH*RLMGQLQPMTQDLIV LDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*YR SPGFSRFLTCSLVLCFFKPILLYEYIHDICNKC LEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A* SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYLF ASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGSK RRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVEK LILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGRC L*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*SS MSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK GV*AHTELCLACLCSSRHPVGTLVVFVVN*IESI VGLKLLKLEHPLSFSAGASWSVLYLAFFLPVLA SPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAMT PLHKEH*TVRGVLFQTKQKCL*V*SELSQVITF LY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTLI LPIINLLNSNTNDKLH*IY*L*KLQSRLVASTQ PFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY FMWHEFRICSDPSECFLPVRIHILCIPAYPELPV PEQAPVFCYFR*QGLAQGQV*ITKVANVCLKTS EMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN LDQKI

Sequence MAP4K4.aAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCA GTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAG GGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCA TGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGCCAGCCATCATGAG AGCAATGGCTTTGCCGGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCTCCACCTCCTCCTCCCCATCCTCCAGCC AGCCGACACCCACCATGTCCCCACAGACACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTC CTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGA CCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAAT ACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGG CCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGAT AAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGG AAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCC ATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATC TATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATG CAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGT AGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAA ACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTG GTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCA AGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTC CCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAG ATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCT GTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGC TGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAG GTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGT CCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAA AAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCA CTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCAC ATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCT TAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGC AGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTG TTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGT TTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATAT CGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAAT GTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTC CTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCT TGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGT TTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATC TGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGT AAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAA AATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTT AGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGA TGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCT CCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAA AGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCC ATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAG CCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATT CTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATG ACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCT TTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATG GTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTA ATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACAC AGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTA TTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCC GTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCT CAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAA TTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV* ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS APGKCSEKT*R*KGSFQTPQAC*SDRTGQRASS SGRCTATSQSNGLLLIQ*GVGDDG*GGRRCGAGR G*RVHLRTRGHQSSVISEFEQW*NGICENHDCP **CRK*AGHDPIQGGHSNRPPEYS*PKACQPS* EQWLCRSHSPLARSLTAKPFLLHFLHLLLPILQP ADTHHVPTDTPGQAHC**DSVR**HTPETQIFL LLYTFYRPQITTDFSI*RNNSDICGGIFL*WDE TRSHKARSYPERLSGQCESYQH*ATE*HPGDS*I QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKW PREGLSSYQPKTISTNGRT*GLECLGDNIWQKG *VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKA ISQIYGL*VIWRIGT*AITGGSHC*GRPEVESD LWILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGC SSTVGRDAYISSIYSIQSDNGLGREGHRDPICG NWSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESS RS*ELGIPCNWSSELHRGQPGQLCVQTSCVGFS PLLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDS VLWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGV LKQRADLI**H*QCI*LTFLFCNVTICGQRRRCR FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRG PILFLWIWGGGPGQELSHFSVYQVA*IMCRWF* KKKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNH MFIFFLPTSLFLMH*EVI*FICFWKKPLMFNLI LIPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISL FSM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGE FPFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVIN VLRKNLFHGFMVITQAPVRILPSQERIRTPCHS PAILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTC LLPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKH LVRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLK N*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV *AFHKMKHATVNTLVYISREEGKAQVENSKRWAD VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEA PCL*VKCYTKKNANGVSLELPSA*NLLAFP*VV KGSELTPSCAWLACAAPGTRWALWWCLW*TELNP LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS* PHPLVNQIELL*LDAGTQ*SFSVKDWESSKCLD SFRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ* HHYIRNIELLEECSSKQNKNVSRFSQSFHK**P FCIKIRVTLSVLSAVFFTLFSCTCYVGENVYKN GFVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP* FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVH SPFFCSANLSTVYYENYCCPPTLFSLNKVKMTP ILCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RP QR*SGR*TSITRFLKTVWSKAGP*RNVTFLQF* IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ RLENAVKKPEDKKEVFRPLKPADLTALAKELRA VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQE GADESTSGPEDTRAASSLNLSNGETESVKTMIVH DDVESEPAMTPSKEGTLIVRQSTVDQKRASHHE SNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSS QPTPTMSPQTPQDKLTANETQSASSTLQKHKSSS SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMR PEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRK YKKRFNSEILCAALWGVNLLVGTESGLMLLDRSG QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKD KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDL EGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDV VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVE TGHLDGVFMHKRAQRLKFLCERNDKVFFASVRS GGSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSL PSFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCS DSTIRCYKCLGLSIILESKHLSSSSRIPCL*MTL SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC *SREQI*YSNINSVFN*HFFFVM*RYVDKEEDA GLRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSK KKKKKKKKRKKKKKKKTCAFCIMARTLSCDSIS TASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S *YQNDQIEV*LLLSQISRHKNCP*RWEIALRLM QFTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGS FPFLKMQCTKTILRM*LILLIHKVGIFCVLGVI SKSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTV LPSYFPLVEF*VEITALWLL*PQSSKETCCFLG LIFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNI W*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK *KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K INPTFKGI*GRLDSIATGL*LELNSCL*FISMF RLFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQ MSLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW* RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN*I HCWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*I LLGKELFSPLLQGSNFSTEVPIWQNYTRKQYCN DTIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKM VLLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYP NFANY*FAK**YK*QTALNLLIIKAAKQTGGKYT ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HL FYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*AS RTRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFE FGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL SAWKMQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQ WKMYGHLTK*RTTPHPVRSRGRRMRRTTMWSRK GLTSPPQDQRTPEQRHL*I*AMVKRNL*KP*LS MMM*KVSRP*PHPRRAL*SSARVQLTKSVPAIMR AMALPVAFTSCQISYSKAIPPPLPPPPPPHPPA SRHPPCPHRHPRTSSLLMRLSPLVAHSRNTNLP PPLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVN TRRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEV AKGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIW KDVYIIKL*NMKESNFW*LL*RVLWKSMRGHQS HITNLWPLSHLENWYISHYWWISLLRKARG*K*S MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPM QSSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM *FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGL VAAVRFIS*P*AGLLF*AGRSSVIQGLLASRVF KILRTWNSL*LELGAAPRATRTAVCADLMCWVLS PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQ ILPSGAISVWD*ASYWKANTFPPAPEFLVSE*L CLVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQ V*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKG SHFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLA LPQLKV*FLFKPRRATKVLPQSALAEERKKFKP HVYFFFAYLIVLNALRGDLVYMFLEETINV*FNL NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*C SLLTSLETRMLCFYQIHH*RLMGQLQPMTQDLI VLDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*Y RSPGFSRFLTCSLVLCFFKPILLYEYIHDICNK CLEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A *SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYL FASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGS KRRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVE KLILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGR CL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*S SMSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK GV*AHTELCLACLCSSRHPVGTLVVFVVN*IES IVGLKLLKLEHPLSFSAGASWSVLYLAFFLPVL ASPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAM TPLHKEH*TVRGVLFQTKQKCL*V*SELSQVIT FLY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTL ILPIINLLNSNTNDKLH*IY*L*KLQSRLVAST QPFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY FMWHEFRICSDPSECFLPVRIHILCIPAYPELP VPEQAPVFCYFR*QGLAQGQV*ITKVANVCLKT SEMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN LDQKI

Sequence MAP4K4.bAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA GGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTC CTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCG AGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGAC AGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCA CAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGTTTCCCCTGTCTCGCGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTTGCACACCACC ATCTTCGTTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGTGCTCAGTCAGAGCTCTGACTCTAAGTCAGAGGCGCCTGACCCTACCCAAAAGGCTTG GTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGT GGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCA GTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATC CAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTG GATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGA CGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGG TGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACT CAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAA CAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTAC CAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTA GTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTG AGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGA AGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCT TTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGG TGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACAT TTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAG GGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAA TGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTG TGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAG AAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACC AGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACT CCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACAC CTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGG GCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTT TTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTG TGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTC TGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATT TTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACC ACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTT TATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAA GAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAG GTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAAC TGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCT TATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACC AATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGAT ATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCT TTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCT CTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTT CCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTAT ACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAA AGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTT AGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAA GAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGA TGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATT ACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGG TGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTC CTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAG TTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGT GCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAA ATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTT CTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGG GTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTA CTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCC CCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGA GGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCA GGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAA AGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA

MAP4K4-F1 (forward primer)
MAP4K4-R1 (reverse primer)
Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP AAPRGEYRAGSAAARGARSAAWSAAEIHRATET FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V *VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR FPETAAGEQGTFRGSSETTVTTGATAPGAGRI* KATAGRETEAD*AAERTEATARRATKERAGS*K AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR RRKEES*KRTGVYQATARRGAAALGSPSAAAAP GAGHVTGVPMAGDGGAPAGREAPEAVATRTSIS PVSTA*P*EAAPAALAAAATTAAGKEQAKLPCSR AQSPLRAC*PSARGGR*I*EN*PQLP*SPV*AD RQSIGATSAFPIRVFFQWQLRVCASRPAETSGA TGTVVPPGISQEQCFPCLAIPFLQ*PFSQICTPP SSFSGPMSTFPQ*GAQSEL*L*VRGA*PYPKGL V*IRQ*RGASKGSCENNISLPCSVPSRFPTAGQ WAAE*PGRTEKLHQQY*AQASVGESGEAGAQTWQ WQLLRVQQLRIPARVSPWVSEWLRGTLQSEIII QV*RLSISAPGKCSEKT*R*KGSFQTPQACWRS GSDRTGQRASSSGRCTATSQSNGLLLIQ*GVGDD G*GGRRCGAGRG*RVHLRTRGHQSSVISEFEQW *NGICENHDCP**CRK*AGHDPIQGGHSNRPPD SVR**HTPETQIFLLLYTFYRPQITTDFSI*RNN SDICGGIFL*WDETRSHKARSYPERLSGQCESY QH*ATE*HPGDS*IQEEV*L*DSVCCLMGSEFA SGYREWPDAAGQKWPREGLSSYQPKTISTNGRT* GLECLGDNIWQKG*VTCLLFVLVKK*NTSQ*SR S*EEAGMDNRRGFGRMCTL*SCKI*KNQISGDC FEEFCGSLCVGTKAISQIYGL*VIWRIGT*AITG GSHC*GRPEVESDLWILCWIPCC*CGFRISL*H LSTNTYPV*HQTPCNHHPPQYRWNGASGVL*R* GGLCKHIWKDHQGCSSTVGRDAYISSIYSIQSDN GLGREGHRDPICGNWSLGWCVHAQKGSKTKILV *TQ*QGVLCLCSVWWQQSGLFHDLRQDFSSELV EAV*SRDYWPPESSRS*ELGIPCNWSSELHRGQP GQLCVQTSCVGFSPLLPVPLIYQFIPILFFFLT PK*IKAAMQLVLFRFYHQVL*VFGIEHHTGKQT PFLQLQNSLSLNDSVLWVSDSGDDEHAVGFIGSG HKEVRSGGKRSGVLKQRADLI**H*QCI*LTFL FCNVTICGQRRRCRFKKLIFIKCERHSY*DNFF VGGAWEMGWGGLRGPILFLWIWGGGPGQELSHFS VYQVA*IMCRWF*KKKKKKKKKEKKKEKENVCI LYNGQNFVV*QY*HCLS*RFNFCLNLDVQQKFY HSLHLQKKEKNSNHMFIFFLPTSLFLMH*EVI*F ICFWKKPLMFNLILIPKRPD*SLTFIVTNQQAQ ELSMKMGNSLKADAVYLQV*KPECFVFTRFTIR G*WGNCSP*HKISLFSM*RGW*QTGGYIRIVTQT VQCCRNLFLGVGEFPFSKNAMH*NYFKNVVNSA YS*SGHLLCFRCNIEVLAFLVFSLALLFSVFLN QFYFMNIFMTFVINVLRKNLFHGFMVITQAPVRI LPSQERIRTPCHSPAILLSSCRVLSGNNCIMAA LTSVIKRNLLFFRLDLFPLWLIFLYIVKMGDFP SAPTHLNTAAICTCLLPIPLGTHSDLLSVSCSWF HLFEKGPPLSYKHLVRQVHPLMNADTAAGGFVY TCKDKLRSLLFGEVKEDGNGCFICMSLEQC*RP KPPTGL*LT*RRLKN*SYL*RDLR*AGFHRHRTL VRIKFLLVIYIHV*AFHKMKHATVNTLVYISRE EGKAQVENSKRWADVFEEMLNV*C*VGEGREQR MWHNGLNIIQRLEAPCL*VKCYTKKNANGVSLEL PSA*NLLAFP*VVKGSELTPSCAWLACAAPGTR WALWWCLW*TELNPLLGLSY*NWNTLCPSRRGL PGLCFTWLFSFPS*PHPLVNQIELL*LDAGTQ*S FSVKDWESSKCLDSFRKGIIFPPFTG**LLHRS ANMAKLHKKTVLQ*HHYIRNIELLEECSSKQNK NVSRFSQSFHK**PFCIKIRVTLSVLSAVFFTLF SCTCYVGENVYKNGFVTLMRTTYLWFILSDFLW VI*VFVLVVAHLP*FCQLLIC*IVIQMTNCIKF TNYKSCKADWWQVHSPFFCSANLSTVYYENYCCP PTLFSLNKVKMTPILCGMSFEYVLTLQNVSFL* GSIFYAYLPTLSFPYQSRLLYFAISDDRGLPKA RYRLQK*QMFV*RPQR*SGR*TSITRFLKTVWSK AGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV SAAARARSVRPPRGPAGREPRAARERSRARRRR PRPREESTGPARLPREERGRRPGLRLRYTERQRH LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK RQLLAERQKRIEQQKEQRRRLEEQQRREREARR QQEREQRRREQEEKRRLEELERRRKEEEERRRA EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ EQAMLLECRWREMEEHRQAERLQRQLQQEQAYL LSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAP EPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQT GRVLEPPVPSRSESFSNGNSESVHPALQRPAEP QVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHH HLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAW SRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGS GQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPG SGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSS KSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEV DLTALAKELRAVEDVRPPHKVTDYSSSSEESGT TDEEDDDVEQEGADESTSGPEDTRAASSLNLSNG ETESVKTMIVHDDVESEPAMTPSKEGTLIVRQT QSASSTLQKHKSSSSFTPFIDPRLLQISPSSGT TVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPT NTRPQSDTPEIRKYKKRFNSEILCAALWGVNLL VGTESGLMLLDRSGQGKVYPLINRRRFQQMDVL EGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPE VEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIA LKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLL VDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDI YLPTHIQCSIKPHAIIILPNTDGMELLVCYEDE GVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQT MGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLC ERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW* KQCDPGITGLQSLQDPENLEFLVTGARSCTEGN QDSCVCRPHVLGSLPSFLFLLYTSLSPFFFFFLL QNKSRLQCSWCCSDSTIRCYKCLGLSIILESKH LSSSSRIPCL*MTLSCGCLTVATMNMPLVLLAV GTRR*EVVVKGAEC*SREQI*YSNINSVFN*HFF FVM*RYVDKEEDAGLRS*YL*NVKDTVTRITFL WVGLGRWGGVG*GVPFCFFGFGVGVLAKNSVIF LCTRLPKSCADGSKKKKKKKKKRKKKKKKKTCAF CIMARTLSCDSISTASVKGLIFV*T*TCNKSFT TVCTCRRKKKIQTTCLFFFCLPHCS*CIER*FS LYVFGRNH*CLI*S*YQNDQIEV*LLLSQISRHK NCP*RWEIALRLMQFTYKFRNQNALFLPDSPLE VDGATAAHDTRSHCSRCRGVGSRQVVTLE*SHK LFSVAGTFSWGWGSFPFLKMQCTKTILRM*LILL IHKVGIFCVLGVISKSWLFSFSHLLSCSLFF*T NFTL*IYS*HL**MS*ERICFMASWSSLKLP*G YYRLRKGSGLHVTVLPSYFPLVEF*VEITALWLL *PQSSKETCCFLGLIFFLCG*FSCIL*KWGIFP LLPPT*TQQPFVPVCFPSHLAPTLTSCQFPVPG SIFLKKALL*ATNIW*DKYIHS*MQTQQLVVLCI PVKTS*EAYFLGK*KKMEMDVSFV*VWSSAEGQ SRLLVCS*PREG*KINPTFKGI*GRLDSIATGL *LELNSCL*FISMFRLFIR*NMPQ*THSCTYQEK KERHRWRTVKGGQMSLKKCSMSDAKWEKAENKG CGIMVLTLSKDLKLHVCKSNVTQKKMQMVFHWN YQVLRTCWLSHRW*RGLSSHRVVLGLLVQLQAPG GHSGGVCGELN*IHCWA*VTEIGTPFVLLGGGF LVCALLGFFPSRLSLTPLSTRLSCYSLMQGPSE VSPLKIGSRRNV*ILLGKELFSPLLQGSNFSTEV PIWQNYTRKQYCNDTIT*GTLNC*RSALPNKTK MSLGLVRAFTSNNLSVLKSE*PFLY*VQCFLLF SHAHVTLEKMFTKMVLLH*CAPHIYGLF*VTFYG LFRFSS*L*HTYPNFANY*FAK**YK*QTALNL LIIKAAKQTGGKYTALFFAVLTCLLCIMKITVV PPPFFP*IK*K*HLFYVA*VSNMF*PFRMFPSCE DPYFMHTCLP*ASRTRAGSCILLFQMTGACPRP GIDYKSSKCLSKDLRDEVGDEPPSPGS*RQYGV KLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV* APPPVHAPSALRAARPAESPERPESAAEPAAAA RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL L*GISQMKGKLESSLRII*IVPGRREARKMKLSM STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS RSRPCYWSADGGRWRSTGRQRGSRGSCNKNKHIS CLYSMTIGGRTRSTRSSRHHRSRKGASQASMLP SPKPTTSLLTERERWKIDLGKLTTAPLKPSLSR QAEYWSHQCLPDQSLFPMATPSLCIPPCRDQRSH RYSGPTWHLSRTMFPLSRDPIPSVTLLPNLHTT IFVLRTHVHLPAVRCSVRALTLSQRRLTLPKRL GLDQTVTRCLQGFL*EQHLAPLFCPVEIPHCRAV GSRIARQDRETPPAVLSPGFCGREWRSWCPDLA VAAPQGPATQDPSPGLTLGLRVAPGNASE*DHH PSLKALHLSAWKMQ*KNLKIKRKFSDPSSLLAKW I*PHWPKSFEQWKMYGHLTK*RTTPHPVRSRGR RMRRTTMWSRKGLTSPPQDQRTPEQRHL*I*AM VKRNL*KP*LSMMM*KVSRP*PHPRRAL*SSARL SPLVAHSRNTNLPPPLHLL*TPDYYRFLHLAEQ Q*HLWWDFPVMG*DQKP*GKILPGKAQWSM*IL PTLGHRVTPRRFVNTRRGLTLRFCVLPYGE*IC* WVQRVA*CCWTEVAKGRSILLSTEDDFNKWTYL RA*MSW*QYLAKRISYVSTICPG*EIKYFTMIQ KLRRSRDGQP*GIWKDVYIIKL*NMKESNFW*LL *RVLWKSMRGHQSHITNLWPLSHLENWYISHYW WISLLRKARG*K*SMDPVLDSMLLMWIQDQSMT FIYQHISSVASNPMQSSSSPIQMEWSFWCAMKMR GFM*THMEGSPRM*FYSGERCLHQ*HIFDPIRQ WAGERRP*RSDLWKLVTWMVCSCTKGLKD*NSC VNAMTRCSLPLFGLVAAVRFIS*P*AGLLF*AGR SSVIQGLLASRVFKILRTWNSL*LELGAAPRAT RTAVCADLMCWVLSPPSCSSYIPVYPHSFFFSY SKINQGCNAAGAVQILPSGAISVWD*ASYWKANT FPPAPEFLVSE*LCLVGV*QWRR*TCRWFYWQW AQGGEKWW*KERSAEAESRFNIVTLTVYLIDIS FL*CDDMWTKKKMQV*EVNIYKM*KTQLLG*LFC GWGLGDGVGWVKGSHFVSLDLGWGSWPRTQSFF CVPGCLNHVQMVLKKKKKKKKKGKKKRKRKRVH FV*WPELCRVTVLALPQLKV*FLFKPRRATKVLP QSALAEERKKFKPHVYFFFAYLIVLNALRGDLV YMFLEETINV*FNLNTKTTRLKFDFYCHKSAGT RTVHEDGK*P*G*CSLLTSLETRMLCFYQIHH*R LMGQLQPMTQDLIVLDVEGLVADRWLH*NSHTN CSVLQEPFLGGGGVSLF*KCNALKLF*ECS*FC LFIKWASSVF*V*YRSPGFSRFLTCSLVLCFFKP ILLYEYIHDICNKCLEKEFVSWLHGHHSSSRKD ITVSGKDQDSMSQSCHLTFLLSSSEWK*LHYGC FNLSHQKKLAVF*A*SFSFVVNFPVYCENGGFSL CSHPPKHSSHLYLFASHPTWHPL*PLVSFLFLV PSF*KRPSFELQTSGKTSTSTHECRHSSWWFCV YL*RQAEKLTFWGSKRRWKWMFHLYEFGAVLKAK AAYWFVVNLEKVEKLILPLKGFEVGWIPSPQDF S*N*IPACNLYPCLGFS*DETCHSEHTRVHIKR RRKGTGGEQ*KVGRCL*RNAQCLMLSGRRQRTKD VA*WS*HYPKT*SSMSVSQMLHKKKCKWCFIGI TKCLELAGFPIGGKGV*AHTELCLACLCSSRHP VGTLVVFVVN*IESIVGLKLLKLEHPLSFSAGAS WSVLYLAFFLPVLASPPCQPD*VAIA*CRDPVK FLR*RLGVVEMFRFF*ERNYFPPFYRVVTSPQK CQYGKITQENSIAMTPLHKEH*TVRGVLFQTKQK CL*V*SELSQVITFLY*NQSNPFCIECSVFYSF LMHMLRWRKCLQKWFCYTNAHHIFMVYFK*LFM GYLGFRLSCSTLTLILPIINLLNSNTNDKLH*IY *L*KLQSRLVASTQPFFLQC*LVYCVL*KLLLS PHPFFLK*SKNDTYFMWHEFRICSDPSECFLPV RIHILCIPAYPELPVPEQAPVFCYFR*QGLAQGQ V*ITKVANVCLKTSEMKWEMNLHHQVPEDSME* SWSLKKCHIFAVLNLDQKI