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Sequences

Sequence MAP4K4.lAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC
GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA
AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC
GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACC
ACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGG
AAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACT
CCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACT
GCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGA
CCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGAT
CATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGA
TCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL
RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE
GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS
GTWKSFSSSCSRSRPCYCMTIGGRTRSTRSSRH
HRSRKGASQASMLPSPKPTTSLLTERERWKIDLG
KLTTAPLKPSLSRQAEYWSHQCLPDQSLFPMAT
PSLCIPPCRDQRSHRFL*EQHLAPLFCPVEIPH
CRAVGSRIARQDRETPPVLSPGFCGREWRSWCPD
LAVAAPQGPATQDPSPGLTLGLRVAPGNASE*D
HHPSLKALHLSAWKMQ*KNLKIKRKFSDPSSLL
I*PHWPKSFEQW

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY
VATKERAGS*KAAGT*TAKERTRRKEASRGVGE
KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA
ALGSPSAAAAPGAGHVTA*P*EAAPAALAAAAT
TAAGKEQAKLPCSRAQSPLRAC*PSARGGR*I*
EN*PQLP*SPV*ADRQSIGATSAFPIRVFFQWQL
RVCASRPAETSGATGSCENNISLPCSVPSRFPT
AGQWAAE*PGRTEKLHQY*AQASVGESGEAGAQ
TWQWQLLRVQQLRIPARVSPWVSEWLRGTLQSEI
IIQV*RLSISAPGKCSEKT*R*KGSFQTPQAC*
SDRTGQRASSSG

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT
*QQRREREARRQQEREQRRREQEEKRRLEELER
RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ
RHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPP
PQQERSKPSFHAPEPKAHYEPADRAREVEDRFR
KTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGN
SESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPL
QGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPR
PGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVR
SSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAD
LTALAKELRAV

Sequence MAP4K4.eAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA
AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC
GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA
CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA
ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA
GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAG
CTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGG
AGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCAT
GATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAG
AAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTT
CCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACAC
CCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATG
CTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAA
TATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGAC
AACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTAT
GCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCC
AGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTG
TAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGA
AGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAG
AGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTT
TGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACT
GGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTC
ATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATG
CAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCT
TGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGG
TAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGA
CAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGT
GGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCAT
GTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTC
GTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAG
AAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATT
AATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAAT
AGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCAT
GACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTT
CTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTG
TGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCA
TGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAG
GACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAAC
TTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAG
CAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCC
TTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGC
TTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAA
CCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGT
AATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAAC
AGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTA
TCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGC
TTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGG
TGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTT
TTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTC
GTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAG
AAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCT
TTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGA
AAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTT
GTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAG
ACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAA
AGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTG
CCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAA
TGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCAC
ATTTTTGCAGTTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV*
ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS
GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR
TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ
LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS
APGKCSEKT*R*KGSFQTPQACWRSGSDRTGQR
ASSSGRCTATSQSNGLLLIQ*GVGDDG*GGRRCG
AGRG*RVHLRTRGHQSSVISEFEQW*NGICENH
DCP**CRK*AGHDPIQGGHSNRPPDSVR**HTP
ETQIFLLLYTFYRPQITTDFSI*RNNSDICGGIF
L*WDETRSHKARSYPERLSGQCESYQH*ATE*H
PGDS*IQEEV*L*DSVCCLMGSEFASGYREWPD
AAGQKWPREGLSSYQPKTISTNGRT*GLECLGDN
IWQKG*VTCLLFVLVKK*NTSQ*SRS*EEAGMD
NRRGFGRMCTL*SCKI*KNQISGDCFEEFCGSL
CVGTKAISQIYGL*VIWRIGT*AITGGSHC*GRP
EVESDLWILCWIPCC*CGFRISL*HLSTNTYPV
*HQTPCNHHPPQYRWNGASGVL*R*GGLCKHIW
KDHQGCSSTVGRDAYISSIYSIQSDNGLGREGHR
DPICGNWSLGWCVHAQKGSKTKILV*TQ*QGVL
CLCSVWWQQSGLFHDLRQDFSSELVEAV*SRDY
WPPESSRS*ELGIPCNWSSELHRGQPGQLCVQTS
CVGFSPLLPVPLIYQFIPILFFFLTPK*IKAAM
QLVLFRFYHQVL*VFGIEHHTGKQTPFLQLQNS
LSLNDSVLWVSDSGDDEHAVGFIGSGHKEVRSGG
KRSGVLKQRADLI**H*QCI*LTFLFCNVTICG
QRRRCRFKKLIFIKCERHSY*DNFFVGGAWEMG
WGGLRGPILFLWIWGGGPGQELSHFSVYQVA*IM
CRWF*KKKKKKKKKEKKKEKENVCILYNGQNFV
V*QY*HCLS*RFNFCLNLDVQQKFYHSLHLQKK
EKNSNHMFIFFLPTSLFLMH*EVI*FICFWKKPL
MFNLILIPKRPD*SLTFIVTNQQAQELSMKMGN
SLKADAVYLQV*KPECFVFTRFTIRG*WGNCSP
*HKISLFSM*RGW*QTGGYIRIVTQTVQCCRNLF
LGVGEFPFSKNAMH*NYFKNVVNSAYS*SGHLL
CFRCNIEVLAFLVFSLALLFSVFLNQFYFMNIF
MTFVINVLRKNLFHGFMVITQAPVRILPSQERIR
TPCHSPAILLSSCRVLSGNNCIMAALTSVIKRN
LLFFRLDLFPLWLIFLYIVKMGDFPSAPTHLNT
AAICTCLLPIPLGTHSDLLSVSCSWFHLFEKGPP
LSYKHLVRQVHPLMNADTAAGGFVYTCKDKLRS
LLFGEVKEDGNGCFICMSLEQC*RPKPPTGL*L
T*RRLKN*SYL*RDLR*AGFHRHRTLVRIKFLLV
IYIHV*AFHKMKHATVNTLVYISREEGKAQVEN
SKRWADVFEEMLNV*C*VGEGREQRMWHNGLNI
IQRLEAPCL*VKCYTKKNANGVSLELPSA*NLLA
FP*VVKGSELTPSCAWLACAAPGTRWALWWCLW
*TELNPLLGLSY*NWNTLCPSRRGLPGLCFTWL
FSFPS*PHPLVNQIELL*LDAGTQ*SFSVKDWES
SKCLDSFRKGIIFPPFTG**LLHRSANMAKLHK
KTVLQ*HHYIRNIELLEECSSKQNKNVSRFSQS
FHK**PFCIKIRVTLSVLSAVFFTLFSCTCYVGE
NVYKNGFVTLMRTTYLWFILSDFLWVI*VFVLV
VAHLP*FCQLLIC*IVIQMTNCIKFTNYKSCKA
DWWQVHSPFFCSANLSTVYYENYCCPPTLFSLNK
VKMTPILCGMSFEYVLTLQNVSFL*GSIFYAYL
PTLSFPYQSRLLYFAISDDRGLPKARYRLQK*Q
MFV*RPQR*SGR*TSITRFLKTVWSKAGP*RNVT
FLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS
KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA
EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG
QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS
NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ
RLENAVKKPEDKKEVFRPLKPAGEVDLTALAKE
LRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDV
EQEGADESTSGPEDTRAASSLNLSNGETESVKTM
IVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQ
KHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGF
SCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDT
PEIRKYKKRFNSEILCAALWGVNLLVGTESGLM
LLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVT
ISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWT
TVGDLEGCVHYKVVKYERIKFLVIALKSSVEVY
AWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEG
QRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQC
SIKPHAIIILPNTDGMELLVCYEDEGVYVNTYG
RITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAI
EIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFF
ASVRSGGSSQVYFMTLGRTSLLSW*KQCDPGIT
GLQSLQDPENLEFLVTGARSCTEGNQDSCVCRP
HVLGSLPSFLFLLYTSLSPFFFFFLLQNKSRLQC
SWCCSDSTIRCYKCLGLSIILESKHLSSSSRIP
CL*MTLSCGCLTVATMNMPLVLLAVGTRR*EVV
VKGAEC*SREQI*YSNINSVFN*HFFFVM*RYVD
KEEDAGLRS*YL*NVKDTVTRITFLWVGLGRWG
GVG*GVPFCFFGFGVGVLAKNSVIFLCTRLPKS
CADGSKKKKKKKKKRKKKKKKKTCAFCIMARTLS
CDSISTASVKGLIFV*T*TCNKSFTTVCTCRRK
KKIQTTCLFFFCLPHCS*CIER*FSLYVFGRNH
*CLI*S*YQNDQIEV*LLLSQISRHKNCP*RWEI
ALRLMQFTYKFRNQNALFLPDSPLEVDGATAAH
DTRSHCSRCRGVGSRQVVTLE*SHKLFSVAGTF
SWGWGSFPFLKMQCTKTILRM*LILLIHKVGIFC
VLGVISKSWLFSFSHLLSCSLFF*TNFTL*IYS
*HL**MS*ERICFMASWSSLKLP*GYYRLRKGS
GLHVTVLPSYFPLVEF*VEITALWLL*PQSSKET
CCFLGLIFFLCG*FSCIL*KWGIFPLLPPT*TQ
QPFVPVCFPSHLAPTLTSCQFPVPGSIFLKKAL
L*ATNIW*DKYIHS*MQTQQLVVLCIPVKTS*EA
YFLGK*KKMEMDVSFV*VWSSAEGQSRLLVCS*
PREG*KINPTFKGI*GRLDSIATGL*LELNSCL
*FISMFRLFIR*NMPQ*THSCTYQEKKERHRWRT
VKGGQMSLKKCSMSDAKWEKAENKGCGIMVLTL
SKDLKLHVCKSNVTQKKMQMVFHWNYQVLRTCW
LSHRW*RGLSSHRVVLGLLVQLQAPGGHSGGVCG
ELN*IHCWA*VTEIGTPFVLLGGGFLVCALLGF
FPSRLSLTPLSTRLSCYSLMQGPSEVSPLKIGS
RRNV*ILLGKELFSPLLQGSNFSTEVPIWQNYTR
KQYCNDTIT*GTLNC*RSALPNKTKMSLGLVRA
FTSNNLSVLKSE*PFLY*VQCFLLFSHAHVTLE
KMFTKMVLLH*CAPHIYGLF*VTFYGLFRFSS*L
*HTYPNFANY*FAK**YK*QTALNLLIIKAAKQ
TGGKYTALFFAVLTCLLCIMKITVVPPPFFP*I
K*K*HLFYVA*VSNMF*PFRMFPSCEDPYFMHTC
LP*ASRTRAGSCILLFQMTGACPRPGIDYKSSK
CLSKDLRDEVGDEPPSPGS*RQYGVKLVLKEMS
HFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL
SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ
RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD
RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA
TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL
SAWKMQ*KNLKIKRKFSDPSSLLAKWI*PHWPKS
FEQWKMYGHLTK*RTTPHPVRSRGRRMRRTTMW
SRKGLTSPPQDQRTPEQRHL*I*AMVKRNL*KP
*LSMMM*KVSRP*PHPRRAL*SSARLSPLVAHSR
NTNLPPPLHLL*TPDYYRFLHLAEQQ*HLWWDF
PVMG*DQKP*GKILPGKAQWSM*ILPTLGHRVT
PRRFVNTRRGLTLRFCVLPYGE*IC*WVQRVA*C
CWTEVAKGRSILLSTEDDFNKWTYLRA*MSW*Q
YLAKRISYVSTICPG*EIKYFTMIQKLRRSRDG
QP*GIWKDVYIIKL*NMKESNFW*LL*RVLWKSM
RGHQSHITNLWPLSHLENWYISHYWWISLLRKA
RG*K*SMDPVLDSMLLMWIQDQSMTFIYQHISS
VASNPMQSSSSPIQMEWSFWCAMKMRGFM*THME
GSPRM*FYSGERCLHQ*HIFDPIRQWAGERRP*
RSDLWKLVTWMVCSCTKGLKD*NSCVNAMTRCS
LPLFGLVAAVRFIS*P*AGLLF*AGRSSVIQGLL
ASRVFKILRTWNSL*LELGAAPRATRTAVCADL
MCWVLSPPSCSSYIPVYPHSFFFSYSKINQGCN
AAGAVQILPSGAISVWD*ASYWKANTFPPAPEFL
VSE*LCLVGV*QWRR*TCRWFYWQWAQGGEKWW
*KERSAEAESRFNIVTLTVYLIDISFL*CDDMW
TKKKMQV*EVNIYKM*KTQLLG*LFCGWGLGDGV
GWVKGSHFVSLDLGWGSWPRTQSFFCVPGCLNH
VQMVLKKKKKKKKKGKKKRKRKRVHFV*WPELC
RVTVLALPQLKV*FLFKPRRATKVLPQSALAEER
KKFKPHVYFFFAYLIVLNALRGDLVYMFLEETI
NV*FNLNTKTTRLKFDFYCHKSAGTRTVHEDGK
*P*G*CSLLTSLETRMLCFYQIHH*RLMGQLQPM
TQDLIVLDVEGLVADRWLH*NSHTNCSVLQEPF
LGGGGVSLF*KCNALKLF*ECS*FCLFIKWASS
VF*V*YRSPGFSRFLTCSLVLCFFKPILLYEYIH
DICNKCLEKEFVSWLHGHHSSSRKDITVSGKDQ
DSMSQSCHLTFLLSSSEWK*LHYGCFNLSHQKK
LAVF*A*SFSFVVNFPVYCENGGFSLCSHPPKHS
SHLYLFASHPTWHPL*PLVSFLFLVPSF*KRPS
FELQTSGKTSTSTHECRHSSWWFCVYL*RQAEK
LTFWGSKRRWKWMFHLYEFGAVLKAKAAYWFVVN
LEKVEKLILPLKGFEVGWIPSPQDFS*N*IPAC
NLYPCLGFS*DETCHSEHTRVHIKRRRKGTGGE
Q*KVGRCL*RNAQCLMLSGRRQRTKDVA*WS*HY
PKT*SSMSVSQMLHKKKCKWCFIGITKCLELAG
FPIGGKGV*AHTELCLACLCSSRHPVGTLVVFV
VN*IESIVGLKLLKLEHPLSFSAGASWSVLYLAF
FLPVLASPPCQPD*VAIA*CRDPVKFLR*RLGV
VEMFRFF*ERNYFPPFYRVVTSPQKCQYGKITQ
ENSIAMTPLHKEH*TVRGVLFQTKQKCL*V*SEL
SQVITFLY*NQSNPFCIECSVFYSFLMHMLRWR
KCLQKWFCYTNAHHIFMVYFK*LFMGYLGFRLS
CSTLTLILPIINLLNSNTNDKLH*IY*L*KLQSR
LVASTQPFFLQC*LVYCVL*KLLLSPHPFFLK*
SKNDTYFMWHEFRICSDPSECFLPVRIHILCIP
AYPELPVPEQAPVFCYFR*QGLAQGQV*ITKVAN
VCLKTSEMKWEMNLHHQVPEDSME*SWSLKKCH
IFAVLNLDQKI

Sequence MAP4K4.dAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTC
CTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCG
AGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGAC
AGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCA
CAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAA
ACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATC
CCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAA
AATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATG
TACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGA
GTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTA
GAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCT
CCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACC
AGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATAC
AAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCC
AAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAA
GTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAA
GGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCAT
ATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTA
TGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCA
ATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAG
TTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAAC
TGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGT
GGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAG
ATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCC
CTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGAT
TCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGT
CTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTG
AAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGT
TTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCC
CATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAA
AAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACT
GCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACAT
GTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTA
ATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAG
TTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTT
CTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTT
CCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCG
AAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGT
CTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCT
GCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTG
ATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTT
GCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTG
GTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAA
AAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAA
TTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAG
GCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATG
TCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCC
ATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAG
GGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCAT
TGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCC
TCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCT
TTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGAC
ACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTT
CTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGT
TTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAAT
TTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAG
CCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATT
TTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGT
ACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCA
GAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATT
TGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTGVPMAGDGGAPAGREAPEAVATRTSIS
PVSTA*P*EAAPAALAAAATTAAGKEQAKLPCSR
AQSPLRAC*PSARGGR*I*EN*PQLP*SPV*AD
RQSIGATSAFPIRVFFQWQLRVCASRPAETSGA
TGSCENNISLPCSVPSRFPTAGQWAAE*PGRTEK
LHQY*AQASVGESGEAGAQTWQWQLLRVQQLRI
PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG
KCSEKT*R*KGSFQTPQAC*SDRTGQRASSSGRC
TATSQSNGLLLIQ*GVGDDG*GGRRCGAGRG*R
VHLRTRGHQSSVISEFEQW*NGICENHDCP**C
RK*AGHDPIQGGHSNRPPDSVR**HTPETQIFLL
LYTFYRPQITTDFSI*RNNSDICGGIFL*WDET
RSHKARSYPERLSGQCESYQH*ATE*HPGDS*I
QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKWP
REGLSSYQPKTISTNGRT*GLECLGDNIWQKG*
VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG
RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKAI
SQIYGL*VIWRIGT*AITGGSHC*GRPEVESDL
WILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC
NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGCS
STVGRDAYISSIYSIQSDNGLGREGHRDPICGN
WSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW
WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESSR
S*ELGIPCNWSSELHRGQPGQLCVQTSCVGFSP
LLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR
FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDSV
LWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGVL
KQRADLI**H*QCI*LTFLFCNVTICGQRRRCR
FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRGP
ILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*K
KKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H
CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNHM
FIFFLPTSLFLMH*EVI*FICFWKKPLMFNLIL
IPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA
VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISLF
SM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGEF
PFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI
EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVINV
LRKNLFHGFMVITQAPVRILPSQERIRTPCHSP
AILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL
DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTCL
LPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKHL
VRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV
KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLKN
*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV*
AFHKMKHATVNTLVYISREEGKAQVENSKRWAD
VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEAP
CL*VKCYTKKNANGVSLELPSA*NLLAFP*VVK
GSELTPSCAWLACAAPGTRWALWWCLW*TELNP
LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*P
HPLVNQIELL*LDAGTQ*SFSVKDWESSKCLDS
FRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ*
HHYIRNIELLEECSSKQNKNVSRFSQSFHK**PF
CIKIRVTLSVLSAVFFTLFSCTCYVGENVYKNG
FVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP*
FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVHS
PFFCSANLSTVYYENYCCPPTLFSLNKVKMTPI
LCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP
YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RPQ
R*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*I
WIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLECRWREMEEHRQAERLQRQLQQEQAYL
LSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAP
EPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQT
GRVLEPPVPSRSESFSNGNSESVHPALQRPAEP
QVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQR
NSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGS
QPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE
NAVKKPEDKKEVFRPLKPADLTALAKELRAVED
VRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADE
STSGPEDTRAASSLNLSNGETESVKTMIVHDDV
ESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSS
SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRP
EAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKY
KKRFNSEILCAALWGVNLLVGTESGLMLLDRSG
QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDK
LRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE
GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP
YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIY
GSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHA
IIILPNTDGMELLVCYEDEGVYVNTYGRITKDV
VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVET
GHLDGVFMHKRAQRLKFLCERNDKVFFASVRSG
GSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ
DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSLP
SFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCSD
STIRCYKCLGLSIILESKHLSSSSRIPCL*MTL
SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC*
SREQI*YSNINSVFN*HFFFVM*RYVDKEEDAG
LRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV
PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSKK
KKKKKKKRKKKKKKKTCAFCIMARTLSCDSIST
ASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT
CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S*
YQNDQIEV*LLLSQISRHKNCP*RWEIALRLMQ
FTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC
SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGSF
PFLKMQCTKTILRM*LILLIHKVGIFCVLGVIS
KSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M
S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTVL
PSYFPLVEF*VEITALWLL*PQSSKETCCFLGL
IFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV
CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNIW
*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK*
KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K
INPTFKGI*GRLDSIATGL*LELNSCL*FISMFR
LFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQM
SLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL
HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*R
GLSSHRVVLGLLVQLQAPGGHSGGVCGELN*IH
CWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS
LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*IL
LGKELFSPLLQGSNFSTEVPIWQNYTRKQYCND
TIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL
SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKMV
LLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYPN
FANY*FAK**YK*QTALNLLIIKAAKQTGGKYT
ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HLF
YVA*VSNMF*PFRMFPSCEDPYFMHTCLP*ASR
TRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL
RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFEF
GSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYWSADGGRWRSTGRQRGSRGSCNKNKHIS
CLYSMTIGGRTRSTRSSRHHRSRKGASQASMLP
SPKPTTSLLTERERWKIDLGKLTTAPLKPSLSR
QAEYWSHQCLPDQSLFPMATPSLCIPPCRDQRSH
RFL*EQHLAPLFCPVEIPHCRAVGSRIARQDRE
TPPVLSPGFCGREWRSWCPDLAVAAPQGPATQD
PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAWK
MQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQWKM
YGHLTK*RTTPHPVRSRGRRMRRTTMWSRKGLT
SPPQDQRTPEQRHL*I*AMVKRNL*KP*LSMMM*
KVSRP*PHPRRAL*SSARLSPLVAHSRNTNLPP
PLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D
QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVNT
RRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEVA
KGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI
SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIWK
DVYIIKL*NMKESNFW*LL*RVLWKSMRGHQSH
ITNLWPLSHLENWYISHYWWISLLRKARG*K*S
MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPMQ
SSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM*
FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK
LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGLV
AAVRFIS*P*AGLLF*AGRSSVIQGLLASRVFK
ILRTWNSL*LELGAAPRATRTAVCADLMCWVLS
PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQI
LPSGAISVWD*ASYWKANTFPPAPEFLVSE*LC
LVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA
EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQV
*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKGS
HFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK
KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLAL
PQLKV*FLFKPRRATKVLPQSALAEERKKFKPH
VYFFFAYLIVLNALRGDLVYMFLEETINV*FNL
NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*CS
LLTSLETRMLCFYQIHH*RLMGQLQPMTQDLIV
LDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV
SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*YR
SPGFSRFLTCSLVLCFFKPILLYEYIHDICNKC
LEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS
CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A*
SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYLF
ASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS
GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGSK
RRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVEK
LILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL
GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGRC
L*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*SS
MSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK
GV*AHTELCLACLCSSRHPVGTLVVFVVN*IESI
VGLKLLKLEHPLSFSAGASWSVLYLAFFLPVLA
SPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF
F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAMT
PLHKEH*TVRGVLFQTKQKCL*V*SELSQVITF
LY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW
FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTLI
LPIINLLNSNTNDKLH*IY*L*KLQSRLVASTQ
PFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY
FMWHEFRICSDPSECFLPVRIHILCIPAYPELPV
PEQAPVFCYFR*QGLAQGQV*ITKVANVCLKTS
EMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN
LDQKI

Sequence MAP4K4.aAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA
AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC
GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA
CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA
ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA
GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCA
GTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAG
GGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCA
TGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGCCAGCCATCATGAG
AGCAATGGCTTTGCCGGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCTCCACCTCCTCCTCCCCATCCTCCAGCC
AGCCGACACCCACCATGTCCCCACAGACACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTC
CTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGA
CCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAAT
ACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGG
CCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGAT
AAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGG
AAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCC
ATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATC
TATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATG
CAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGT
AGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAA
ACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTG
GTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCA
AGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTC
CCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAG
ATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCT
GTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGC
TGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAG
GTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGT
CCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAA
AAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCA
CTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCAC
ATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCT
TAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGC
AGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTG
TTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGT
TTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATAT
CGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAAT
GTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTC
CTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCT
TGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGT
TTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATC
TGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGT
AAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAA
AATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTT
AGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGA
TGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCT
CCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAA
AGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCC
ATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAG
CCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATT
CTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATG
ACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCT
TTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATG
GTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTA
ATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACAC
AGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTA
TTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCC
GTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCT
CAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAA
TTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV*
ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS
GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR
TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ
LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS
APGKCSEKT*R*KGSFQTPQAC*SDRTGQRASS
SGRCTATSQSNGLLLIQ*GVGDDG*GGRRCGAGR
G*RVHLRTRGHQSSVISEFEQW*NGICENHDCP
**CRK*AGHDPIQGGHSNRPPEYS*PKACQPS*
EQWLCRSHSPLARSLTAKPFLLHFLHLLLPILQP
ADTHHVPTDTPGQAHC**DSVR**HTPETQIFL
LLYTFYRPQITTDFSI*RNNSDICGGIFL*WDE
TRSHKARSYPERLSGQCESYQH*ATE*HPGDS*I
QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKW
PREGLSSYQPKTISTNGRT*GLECLGDNIWQKG
*VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG
RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKA
ISQIYGL*VIWRIGT*AITGGSHC*GRPEVESD
LWILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC
NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGC
SSTVGRDAYISSIYSIQSDNGLGREGHRDPICG
NWSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW
WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESS
RS*ELGIPCNWSSELHRGQPGQLCVQTSCVGFS
PLLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR
FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDS
VLWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGV
LKQRADLI**H*QCI*LTFLFCNVTICGQRRRCR
FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRG
PILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*
KKKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H
CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNH
MFIFFLPTSLFLMH*EVI*FICFWKKPLMFNLI
LIPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA
VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISL
FSM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGE
FPFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI
EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVIN
VLRKNLFHGFMVITQAPVRILPSQERIRTPCHS
PAILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL
DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTC
LLPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKH
LVRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV
KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLK
N*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV
*AFHKMKHATVNTLVYISREEGKAQVENSKRWAD
VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEA
PCL*VKCYTKKNANGVSLELPSA*NLLAFP*VV
KGSELTPSCAWLACAAPGTRWALWWCLW*TELNP
LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*
PHPLVNQIELL*LDAGTQ*SFSVKDWESSKCLD
SFRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ*
HHYIRNIELLEECSSKQNKNVSRFSQSFHK**P
FCIKIRVTLSVLSAVFFTLFSCTCYVGENVYKN
GFVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP*
FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVH
SPFFCSANLSTVYYENYCCPPTLFSLNKVKMTP
ILCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP
YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RP
QR*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*
IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS
KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA
EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG
QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS
NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ
RLENAVKKPEDKKEVFRPLKPADLTALAKELRA
VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQE
GADESTSGPEDTRAASSLNLSNGETESVKTMIVH
DDVESEPAMTPSKEGTLIVRQSTVDQKRASHHE
SNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSS
QPTPTMSPQTPQDKLTANETQSASSTLQKHKSSS
SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMR
PEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRK
YKKRFNSEILCAALWGVNLLVGTESGLMLLDRSG
QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKD
KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDL
EGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP
YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI
YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH
AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDV
VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVE
TGHLDGVFMHKRAQRLKFLCERNDKVFFASVRS
GGSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ
DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSL
PSFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCS
DSTIRCYKCLGLSIILESKHLSSSSRIPCL*MTL
SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC
*SREQI*YSNINSVFN*HFFFVM*RYVDKEEDA
GLRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV
PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSK
KKKKKKKKRKKKKKKKTCAFCIMARTLSCDSIS
TASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT
CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S
*YQNDQIEV*LLLSQISRHKNCP*RWEIALRLM
QFTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC
SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGS
FPFLKMQCTKTILRM*LILLIHKVGIFCVLGVI
SKSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M
S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTV
LPSYFPLVEF*VEITALWLL*PQSSKETCCFLG
LIFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV
CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNI
W*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK
*KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K
INPTFKGI*GRLDSIATGL*LELNSCL*FISMF
RLFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQ
MSLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL
HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*
RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN*I
HCWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS
LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*I
LLGKELFSPLLQGSNFSTEVPIWQNYTRKQYCN
DTIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL
SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKM
VLLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYP
NFANY*FAK**YK*QTALNLLIIKAAKQTGGKYT
ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HL
FYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*AS
RTRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL
RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFE
FGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL
SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ
RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD
RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA
TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL
SAWKMQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQ
WKMYGHLTK*RTTPHPVRSRGRRMRRTTMWSRK
GLTSPPQDQRTPEQRHL*I*AMVKRNL*KP*LS
MMM*KVSRP*PHPRRAL*SSARVQLTKSVPAIMR
AMALPVAFTSCQISYSKAIPPPLPPPPPPHPPA
SRHPPCPHRHPRTSSLLMRLSPLVAHSRNTNLP
PPLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D
QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVN
TRRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEV
AKGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI
SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIW
KDVYIIKL*NMKESNFW*LL*RVLWKSMRGHQS
HITNLWPLSHLENWYISHYWWISLLRKARG*K*S
MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPM
QSSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM
*FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK
LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGL
VAAVRFIS*P*AGLLF*AGRSSVIQGLLASRVF
KILRTWNSL*LELGAAPRATRTAVCADLMCWVLS
PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQ
ILPSGAISVWD*ASYWKANTFPPAPEFLVSE*L
CLVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA
EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQ
V*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKG
SHFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK
KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLA
LPQLKV*FLFKPRRATKVLPQSALAEERKKFKP
HVYFFFAYLIVLNALRGDLVYMFLEETINV*FNL
NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*C
SLLTSLETRMLCFYQIHH*RLMGQLQPMTQDLI
VLDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV
SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*Y
RSPGFSRFLTCSLVLCFFKPILLYEYIHDICNK
CLEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS
CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A
*SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYL
FASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS
GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGS
KRRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVE
KLILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL
GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGR
CL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*S
SMSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK
GV*AHTELCLACLCSSRHPVGTLVVFVVN*IES
IVGLKLLKLEHPLSFSAGASWSVLYLAFFLPVL
ASPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF
F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAM
TPLHKEH*TVRGVLFQTKQKCL*V*SELSQVIT
FLY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW
FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTL
ILPIINLLNSNTNDKLH*IY*L*KLQSRLVAST
QPFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY
FMWHEFRICSDPSECFLPVRIHILCIPAYPELP
VPEQAPVFCYFR*QGLAQGQV*ITKVANVCLKT
SEMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN
LDQKI

Sequence MAP4K4.mAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC
GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA
AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC
GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCT
CCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAG
CAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTACAGTGGTCCCACCTGGCATCTC
TCAAGAACAATGTTTCCCCTGTCTCGCGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTTGCACACCACCATCTTCGTTCTCAGGACCCATGTCCACC
TTCCCGCAGTGAGGTGCTCAGTCAGAGCTCTGACTCTAAGTCAGAGGCGCCTGACCCTACCCAAAAGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCT
CCAAGGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGA
GAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL
RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE
GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS
GTWKSFSSSCSRSRPCYWSADGGRWRSTGRQRG
SRGSCNKNKHISCLYSMTIGGRTRSTRSSRHHRS
RKGASQASMLPSPKPTTSLLTERERYSGPTWHL
SRTMFPLSRDPIPSVTLLPNLHTTIFVLRTHVH
LPAVRCSVRALTLSQRRLTLPKRLGLDQTVTRCL
QGFL*EQHLAPLFCPVEIPHCRAVGSRIARQDR
ETPPVLSPGFCGREWR

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY
VATKERAGS*KAAGT*TAKERTRRKEASRGVGE
KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA
ALGSPSAAAAPGAGHVTGVPMAGDGGAPAGREA
PEAVATRTSISPVSTA*P*EAAPAALAAAATTA
AGKEQAKLPCSRAQSPLRAC*PSARGTVVPPGIS
QEQCFPCLAIPFLQ*PFSQICTPPSSFSGPMST
FPQ*GAQSEL*L*VRGA*PYPKGLV*IRQ*RGA
SKGSCENNISLPCSVPSRFPTAGQWAAE*PGRTE
KLHQY*AQASVGESG

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT
*QQRREREARRQQEREQRRREQEEKRRLEELER
RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ
RHLEVLQQQLLQEQAMLLECRWREMEEHRQAERL
QRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQ
QERSKPSFHAPEPKAHYEPADRAREVQWSHLAS
LKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPP
SRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVP
PRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQ
RNSTSIEPRLLWERVE

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