Back to Rnomics Platform
 Open Session
×
1 warning:
  • Access restricted to public data.
    Please sign in at Rnomics Platform to access your data.
Navigation
Home
Sequences

Sequence MAP4K4.iAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC
GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA
AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC
GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACC
ACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACA
TCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGC
CCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCC
TGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCT
GAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAG
TAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGA
GGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATG
ACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAG
ACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGA
TCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCT
GAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTC
TTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTT
GTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAA
GTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCT
TTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATT
CCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAAT
ACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGA
TGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGT
GTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTAT
TTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAAT
TCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTT
ATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTAT
AAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAG
TGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTA
ATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTA
TAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGAT
TTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAA
AAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTA
ATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCC
TACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGAT
TGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGA
AACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTG
GTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCA
ATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCG
TTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTT
TCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCT
TGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGT
TAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGG
CACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCA
CTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGAT
GTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAG
GGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAA
CATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCA
ATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATG
TTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCG
AGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACT
GAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCA
GATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATT
TTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACA
TTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGT
AACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGC
ACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTT
GCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGC
TAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTC
GAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGT
ATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATG
AACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL
RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE
GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS
GTWKSFSSSCSRSRPCYCMTIGGRTRSTRSSRH
HRSRKGASQASMLPSPKPTTSLLTERERFL*EQH
LAPLFCPVEIPHCRAVGSRIARQDRETPPAVLS
PGFCGREWRSWCPDLAVAAPQGPATQDPSPGLT
LGLRVAPGNASE*DHHPSLKALHLSAWKMQ*KNL
KIKRKFSDPSSLLI*PHWPKSFEQWKMYGHLTK
*RTTPHPVRSRGRRMRRTTMWSRKGLTSPPQDQ
RTPEQRHL*I*AMVKRNL*KP*LSMMM*KVSRP*
PHPRRAL*SSARLSPLVAHSRNTNLPPPLHLL*
TPDYYRFLHLAEQQ*HLWWDFPVMG*DQKP*GK
ILPGKAQWSM*ILPTLGHRVTPRRFVNTRRGLTL
RFCVLPYGE*IC*WVQRVA*CCWTEVAKGRSIL
LSTEDDFNKWTYLRA*MSW*QYLAKRISYVSTI
CPG*EIKYFTMIQKLRRSRDGQP*GIWKDVYIIK
L*NMKESNFW*LL*RVLWKSMRGHQSHITNLWP
LSHLENWYISHYWWISLLRKARG*K*SMDPVLD
SMLLMWIQDQSMTFIYQHISSVASNPMQSSSSPI
QMEWSFWCAMKMRGFM*THMEGSPRM*FYSGER
CLHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMV
CSCTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFI
S*P*AGLLF*AGRSSVIQGLLASRVFKILRTWN
SL*LELGAAPRATRTAVCADLMCWVLSPPSCSS
YIPVYPHSFFFSYSKINQGCNAAGAVQILPSGAI
SVWD*ASYWKANTFPPAPEFLVSE*LCLVGV*Q
WRR*TCRWFYWQWAQGGEKWW*KERSAEAESRF
NIVTLTVYLIDISFL*CDDMWTKKKMQV*EVNIY
KM*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLD
LGWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKK
KKGKKKRKRKRVHFV*WPELCRVTVLALPQLKV*
FLFKPRRATKVLPQSALAEERKKFKPHVYFFFA
YLIVLNALRGDLVYMFLEETINV*FNLNTKTTR
LKFDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLE
TRMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGL
VADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KC
NALKLF*ECS*FCLFIKWASSVF*V*YRSPGFSR
FLTCSLVLCFFKPILLYEYIHDICNKCLEKEFV
SWLHGHHSSSRKDITVSGKDQDSMSQSCHLTFL
LSSSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVV
NFPVYCENGGFSLCSHPPKHSSHLYLFASHPTW
HPL*PLVSFLFLVPSF*KRPSFELQTSGKTSTS
THECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWM
FHLYEFGAVLKAKAAYWFVVNLEKVEKLILPLK
GFEVGWIPSPQDFS*N*IPACNLYPCLGFS*DE
TCHSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQ
CLMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQM
LHKKKCKWCFIGITKCLELAGFPIGGKGV*AHT
ELCLACLCSSRHPVGTLVVFVVN*IESIVGLKLL
KLEHPLSFSAGASWSVLYLAFFLPVLASPPCQP
D*VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNY
FPPFYRVVTSPQKCQYGKITQENSIAMTPLHKEH
*TVRGVLFQTKQKCL*V*SELSQVITFLY*NQS
NPFCIECSVFYSFLMHMLRWRKCLQKWFCYTNA
HHIFMVYFK*LFMGYLGFRLSCSTLTLILPIINL
LNSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC
*LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEF
RICSDPSECFLPVRIHILCIPAYPELPVPEQAPV
FCYFR*QGLAQGQV*ITKVANVCLKTSEMKWEM
NLHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY
VATKERAGS*KAAGT*TAKERTRRKEASRGVGE
KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA
ALGSPSAAAAPGAGHVTA*P*EAAPAALAAAAT
TAAGKEQAKLPCSRAQSPLRAC*PSARGSCENN
ISLPCSVPSRFPTAGQWAAE*PGRTEKLHQQY*A
QASVGESGEAGAQTWQWQLLRVQQLRIPARVSP
WVSEWLRGTLQSEIIIQV*RLSISAPGKCSEKT
*R*KGSFQTPQAC*SDRTGQRASSSGRCTATSQS
NGLLLIQ*GVGDDG*GGRRCGAGRG*RVHLRTR
GHQSSVISEFEQW*NGICENHDCP**CRK*AGH
DPIQGGHSNRPPDSVR**HTPETQIFLLLYTFYR
PQITTDFSI*RNNSDICGGIFL*WDETRSHKAR
SYPERLSGQCESYQH*ATE*HPGDS*IQEEV*L
*DSVCCLMGSEFASGYREWPDAAGQKWPREGLSS
YQPKTISTNGRT*GLECLGDNIWQKG*VTCLLF
VLVKK*NTSQ*SRS*EEAGMDNRRGFGRMCTL*
SCKI*KNQISGDCFEEFCGSLCVGTKAISQIYGL
*VIWRIGT*AITGGSHC*GRPEVESDLWILCWI
PCC*CGFRISL*HLSTNTYPV*HQTPCNHHPPQ
YRWNGASGVL*R*GGLCKHIWKDHQGCSSTVGRD
AYISSIYSIQSDNGLGREGHRDPICGNWSLGWC
VHAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGL
FHDLRQDFSSELVEAV*SRDYWPPESSRS*ELGI
PCNWSSELHRGQPGQLCVQTSCVGFSPLLPVPL
IYQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL
*VFGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDS
GDDEHAVGFIGSGHKEVRSGGKRSGVLKQRADL
I**H*QCI*LTFLFCNVTICGQRRRCRFKKLIF
IKCERHSY*DNFFVGGAWEMGWGGLRGPILFLWI
WGGGPGQELSHFSVYQVA*IMCRWF*KKKKKKK
KKEKKKEKENVCILYNGQNFVV*QY*HCLS*RF
NFCLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLP
TSLFLMH*EVI*FICFWKKPLMFNLILIPKRPD
*SLTFIVTNQQAQELSMKMGNSLKADAVYLQV*
KPECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW
*QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNA
MH*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFL
VFSLALLFSVFLNQFYFMNIFMTFVINVLRKNLF
HGFMVITQAPVRILPSQERIRTPCHSPAILLSS
CRVLSGNNCIMAALTSVIKRNLLFFRLDLFPLW
LIFLYIVKMGDFPSAPTHLNTAAICTCLLPIPLG
THSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHP
LMNADTAAGGFVYTCKDKLRSLLFGEVKEDGNG
CFICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*R
DLR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMK
HATVNTLVYISREEGKAQVENSKRWADVFEEML
NV*C*VGEGREQRMWHNGLNIIQRLEAPCL*VKC
YTKKNANGVSLELPSA*NLLAFP*VVKGSELTP
SCAWLACAAPGTRWALWWCLW*TELNPLLGLSY
*NWNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQ
IELL*LDAGTQ*SFSVKDWESSKCLDSFRKGII
FPPFTG**LLHRSANMAKLHKKTVLQ*HHYIRN
IELLEECSSKQNKNVSRFSQSFHK**PFCIKIRV
TLSVLSAVFFTLFSCTCYVGENVYKNGFVTLMR
TTYLWFILSDFLWVI*VFVLVVAHLP*FCQLLI
C*IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSA
NLSTVYYENYCCPPTLFSLNKVKMTPILCGMSF
EYVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLL
YFAISDDRGLPKARYRLQK*QMFV*RPQR*SGR*
TSITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT
*QQRREREARRQQEREQRRREQEEKRRLEELER
RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ
RHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPP
PQQERSKPSFHAPEPKAHYEPADRAREVPVRTT
SRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIE
PRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHP
GSQSGSGERFRVRSSSKSEGSPSQRLENAVKKP
EDKKEVFRPLKPADLTALAKELRAVEDVRPPHK
VTDYSSSSEESGTTDEEDDDVEQEGADESTSGPE
DTRAASSLNLSNGETESVKTMIVHDDVESEPAM
TPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFI
DPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQD
PTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNS
EILCAALWGVNLLVGTESGLMLLDRSGQGKVYP
LINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYL
SWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYK
VVKYERIKFLVIALKSSVEVYAWAPKPYHKFMA
FKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGF
HAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPN
TDGMELLVCYEDEGVYVNTYGRITKDVVLQWGE
MPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGV
FMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY
FMTLGRTSLLSW*KQCDPGITGLQSLQDPENLE
FLVTGARSCTEGNQDSCVCRPHVLGSLPSFLFLL
YTSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCY
KCLGLSIILESKHLSSSSRIPCL*MTLSCGCLT
VATMNMPLVLLAVGTRR*EVVVKGAEC*SREQI*
YSNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL
*NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFG
FGVGVLAKNSVIFLCTRLPKSCADGSKKKKKKKK
KRKKKKKKKTCAFCIMARTLSCDSISTASVKGL
IFV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFC
LPHCS*CIER*FSLYVFGRNH*CLI*S*YQNDQI
EV*LLLSQISRHKNCP*RWEIALRLMQFTYKFR
NQNALFLPDSPLEVDGATAAHDTRSHCSRCRGV
GSRQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQ
CTKTILRM*LILLIHKVGIFCVLGVISKSWLFS
FSHLLSCSLFF*TNFTL*IYS*HL**MS*ERIC
FMASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPL
VEF*VEITALWLL*PQSSKETCCFLGLIFFLCG
*FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHL
APTLTSCQFPVPGSIFLKKALL*ATNIW*DKYIH
S*MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMD
VSFV*VWSSAEGQSRLLVCS*PREG*KINPTFK
GI*GRLDSIATGL*LELNSCL*FISMFRLFIR*N
MPQ*THSCTYQEKKERHRWRTVKGGQMSLKKCS
MSDAKWEKAENKGCGIMVLTLSKDLKLHVCKSN
VTQKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHR
VVLGLLVQLQAPGGHSGGVCGELN*IHCWA*VT
EIGTPFVLLGGGFLVCALLGFFPSRLSLTPLST
RLSCYSLMQGPSEVSPLKIGSRRNV*ILLGKELF
SPLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GT
LNC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE
*PFLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CA
PHIYGLF*VTFYGLFRFSS*L*HTYPNFANY*F
AK**YK*QTALNLLIIKAAKQTGGKYTALFFAV
LTCLLCIMKITVVPPPFFP*IK*K*HLFYVA*VS
NMF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSC
ILLFQMTGACPRPGIDYKSSKCLSKDLRDEVGD
EPPSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Sequence MAP4K4.hAug05

GAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCAT
TACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATT
CATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACA
GGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAG
AAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCC
AGAAACCCTCCTCCCCGGCTGAAGTCAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTA
CAGAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGC
CTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTC
CGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAG
CAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAG
AAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTA
TATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAG
ATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGC
AGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCG
AGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGA
AACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGAT
CCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGA
AAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGAT
GTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACG
AGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGT
AGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCC
TCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGAC
CAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATA
CAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGC
CAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATA
AGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGA
AGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCA
TATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCT
ATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGC
AATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTA
GTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAA
CTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGG
TGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAA
GATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCC
CCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGA
TTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTG
TCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCT
GAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGG
TTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTC
CCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAA
AAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCAC
TGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACA
TGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTT
AATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCA
GTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGT
TCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTT
TCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATC
GAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATG
TCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCC
TGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTT
GATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTT
TGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCT
GGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTA
AAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAA
ATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTA
GGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGAT
GTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTC
CATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAA
GGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCA
TTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGC
CTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTC
TTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGA
CACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTT
TCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGG
TTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAA
TTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACA
GCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTAT
TTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCG
TACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTC
AGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAAT
TTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

ETLQHIMVLSSKRALQDMMTNSGLLWSSVGLGP
LQTL*RTPKGTHSKKTGSLTSPEKS*GDWHIFTF
IM*FTGISRARMCC*LRMQR*NLLTLV*VLSWT
GLWGGEIRS*ALPTGWLLRSSPVMRTQMPPMIT
EVIFGLVALQPLRWQKVLPLSVTCIQ*EHCFSFP
ETLLPG*SQKNGRRSFLVL*KGAW*RITCSGPL
QSSF*NILL*GISQMKDETEYEYSGSEEEEEEV
PEQEGEPSSIVNVPGESTLRRDFLRLQQENKERS
EALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQE
EKRRLEELERRRKEEEERRRAEEEKRRVEREQEY
IRRQLEEEQRHLEVLQQQLLQEQAMLLECRWRE
MEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHP
QHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAR
EVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQR
NSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSG
SQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE
NAVKKPEDKKEVFRPLKPADLTALAKELRAVED
VRPPHKVTDYSSSSEESGTTDEEDDDVEQEGAD
ESTSGPEDTRAASSLNLSNGETESVKTMIVHDDV
ESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSS
SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMR
PEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKY
KKRFNSEILCAALWGVNLLVGTESGLMLLDRSG
QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKD
KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE
GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP
YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI
YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHA
IIILPNTDGMELLVCYEDEGVYVNTYGRITKDV
VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVE
TGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSG
GSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ
DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSL
PSFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCSD
STIRCYKCLGLSIILESKHLSSSSRIPCL*MTL
SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC
*SREQI*YSNINSVFN*HFFFVM*RYVDKEEDAG
LRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV
PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSK
KKKKKKKKRKKKKKKKTCAFCIMARTLSCDSIST
ASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT
CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S
*YQNDQIEV*LLLSQISRHKNCP*RWEIALRLMQ
FTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC
SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGS
FPFLKMQCTKTILRM*LILLIHKVGIFCVLGVIS
KSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M
S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTV
LPSYFPLVEF*VEITALWLL*PQSSKETCCFLGL
IFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV
CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNI
W*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK*
KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K
INPTFKGI*GRLDSIATGL*LELNSCL*FISMF
RLFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQM
SLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL
HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*
RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN*IH
CWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS
LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*I
LLGKELFSPLLQGSNFSTEVPIWQNYTRKQYCND
TIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL
SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKM
VLLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYPN
FANY*FAK**YK*QTALNLLIIKAAKQTGGKYT
ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HL
FYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*ASR
TRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL
RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFE
FGSKN

Frame 1

KHCNILWCFHQKEPSRT**PTLACYGVLWGWVH
YRPCEEHQREHTQRRLDRLHLQRNPEGTGTSSH
SSCDSPGYQGPECVAD*ECRGETC*LWCECSAGQ
DCGAEKYVHRHSLLDGS*GHRL**EPRCHL*LQ
K*SLVLWHYSH*DGRRCSPSL*HASNESTVSHS
QKPSSPAEVKKMVEEVF*FYRRVPGEELHAAALY
RAAFETSFYKGSAK*KMKLSMSTVGVRKKRRKC
LNRKESQVPL*TCLVSLLFAEIS*DCSRRTRNV
PRLFGDNSYYRSNSSGSRKNIKGNCWQRDRSGLS
SRKNRGDG*KSNKGESGKLEGSRNVNSEGENKK
KRGV*RSWREGAKKKRRGDGQKKKRGELKENRS
ISGDS*KRSSGTWKSFSSSCSRSRPCYWSADGGR
WRSTGRQRGSRGSCNKNKHISCLYSMTIGGRTR
STRSSRHHRSRKGASQASMLPSPKPTTSLLTER
ERFL*EQHLAPLFCPVEIPHCRAVGSRIARQDRE
TPPVLSPGFCGREWRSWCPDLAVAAPQGPATQD
PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAW
KMQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQWKM
YGHLTK*RTTPHPVRSRGRRMRRTTMWSRKGLT
SPPQDQRTPEQRHL*I*AMVKRNL*KP*LSMMM
*KVSRP*PHPRRAL*SSARLSPLVAHSRNTNLPP
PLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D
QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVN
TRRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEVA
KGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI
SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIW
KDVYIIKL*NMKESNFW*LL*RVLWKSMRGHQSH
ITNLWPLSHLENWYISHYWWISLLRKARG*K*S
MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPM
QSSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM*
FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK
LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGL
VAAVRFIS*P*AGLLF*AGRSSVIQGLLASRVFK
ILRTWNSL*LELGAAPRATRTAVCADLMCWVLS
PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQ
ILPSGAISVWD*ASYWKANTFPPAPEFLVSE*LC
LVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA
EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQ
V*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKGS
HFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK
KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLA
LPQLKV*FLFKPRRATKVLPQSALAEERKKFKPH
VYFFFAYLIVLNALRGDLVYMFLEETINV*FNL
NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*C
SLLTSLETRMLCFYQIHH*RLMGQLQPMTQDLIV
LDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV
SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*Y
RSPGFSRFLTCSLVLCFFKPILLYEYIHDICNKC
LEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS
CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A
*SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYLF
ASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS
GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGS
KRRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVEK
LILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL
GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGR
CL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*SS
MSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK
GV*AHTELCLACLCSSRHPVGTLVVFVVN*IES
IVGLKLLKLEHPLSFSAGASWSVLYLAFFLPVLA
SPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF
F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAM
TPLHKEH*TVRGVLFQTKQKCL*V*SELSQVITF
LY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW
FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTL
ILPIINLLNSNTNDKLH*IY*L*KLQSRLVASTQ
PFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY
FMWHEFRICSDPSECFLPVRIHILCIPAYPELP
VPEQAPVFCYFR*QGLAQGQV*ITKVANVCLKTS
EMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN
LDQKI

Frame 2

NIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSI
TDLVKNTKGNTLKEDWIAYISREILRGLAHLHI
HHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLD
RTVGRRNTFIGTPYWMAPEVIACDENPDATYDYR
SDLWSCGITAIEMAEGAPPLCDMHPMRALFLIP
RNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPS
TEQLLKHPFIRDQPNER*N*V*VQWE*GRRGGSA
*TGRRAKFHCERAW*VYSSPRFPETAAGEQGTF
RGSSETTVTTGATAPGAGRI*KATAGRETEAD*
AAERTEATARRATKERAGS*KAAGT*TAKERTRR
KEASRGVGEKAQRRRGEETGRRRKEES*KRTGV
YQATARRGAAALGSPSAAAAPGAGHVTGVPMAG
DGGAPAGREAPEAVATRTSISPVSTA*P*EAAPA
ALAAAATTAAGKEQAKLPCSRAQSPLRAC*PSA
RGSCENNISLPCSVPSRFPTAGQWAAE*PGRTE
KLHQY*AQASVGESGEAGAQTWQWQLLRVQQLRI
PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG
KCSEKT*R*KGSFQTPQAC*SDRTGQRASSSGR
CTATSQSNGLLLIQ*GVGDDG*GGRRCGAGRG*R
VHLRTRGHQSSVISEFEQW*NGICENHDCP**C
RK*AGHDPIQGGHSNRPPDSVR**HTPETQIFL
LLYTFYRPQITTDFSI*RNNSDICGGIFL*WDET
RSHKARSYPERLSGQCESYQH*ATE*HPGDS*I
QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKW
PREGLSSYQPKTISTNGRT*GLECLGDNIWQKG*
VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG
RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKA
ISQIYGL*VIWRIGT*AITGGSHC*GRPEVESDL
WILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC
NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGC
SSTVGRDAYISSIYSIQSDNGLGREGHRDPICGN
WSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW
WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESS
RS*ELGIPCNWSSELHRGQPGQLCVQTSCVGFSP
LLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR
FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDS
VLWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGVL
KQRADLI**H*QCI*LTFLFCNVTICGQRRRCR
FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRG
PILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*K
KKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H
CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNH
MFIFFLPTSLFLMH*EVI*FICFWKKPLMFNLIL
IPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA
VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISL
FSM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGEF
PFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI
EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVIN
VLRKNLFHGFMVITQAPVRILPSQERIRTPCHSP
AILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL
DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTC
LLPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKHL
VRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV
KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLK
N*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV*
AFHKMKHATVNTLVYISREEGKAQVENSKRWAD
VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEA
PCL*VKCYTKKNANGVSLELPSA*NLLAFP*VVK
GSELTPSCAWLACAAPGTRWALWWCLW*TELNP
LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*
PHPLVNQIELL*LDAGTQ*SFSVKDWESSKCLDS
FRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ*
HHYIRNIELLEECSSKQNKNVSRFSQSFHK**P
FCIKIRVTLSVLSAVFFTLFSCTCYVGENVYKNG
FVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP*
FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVH
SPFFCSANLSTVYYENYCCPPTLFSLNKVKMTPI
LCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP
YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RP
QR*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*I
WIKK

Sequence MAP4K4.gAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGA
ACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAG
GCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGC
AGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAA
GAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATC
AGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGG
AGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCA
CTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAG
GTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACT
CCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATC
CCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAA
AATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAG
TGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGG
GGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCAT
GATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAAT
CTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGG
GATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATT
CGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACA
GAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAA
AAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGG
GATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCAC
CAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAA
AGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAA
CCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCA
AGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATC
TGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTT
CGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGA
GTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGG
TTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGC
TGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAA
TGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGC
GGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAA
GATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTT
AAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGG
TTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGT
ATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTC
AAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAAT
TTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGG
CTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGAT
CTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTG
GGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGT
GTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGT
AATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGT
CACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTT
TTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTG
TACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTAC
AAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTG
GGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAG
GTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATC
CATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGT
GGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACT
TGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAG
GTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAAT
TGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCC
GTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGT
TTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTAT
TGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTA
ATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTAC
AAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACT
TACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCA
AGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATG
ACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGA
GCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTA
AAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAG
TTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGET**N*V*VQWE*GRRGGSA*
TGRRAKFHCERAW*VYSSPRFPETAAGEQGTFR
GSSETTVTTGATAPGAGRI*KATAGRETEAD*AA
ERTEATARRATKERAGS*KAAGT*TAKERTRRK
EASRGVGEKAQRRRGEETGRRRKEES*KRTGVY
QATARRGAAALGSPSAAAAPGAGHVTGVPMAGDG
GAPAGREAPEAVATRTSISPVSTA*P*EAAPAA
LAAAATTAAGKEQAKLPCSRAQSPLRAC*PSAR
GSCENNISLPCSVPSRFPTAGQWAAE*PGRTEKL
HQQY*AQASVGESGEAGAQTWQWQLLRVQQLRI
PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG
KCSEKT*R*KGSFQTPQACWRSGSDRTGQRASSS
GRCTATSQSNGLLLIQ*GVGDDG*GGRRCGAGR
G*RVHLRTRGHQSSVISEFEQW*NGICENHDCP
**CRK*AGHDPIQGGHSNRPPDSVR**HTPETQI
FLLLYTFYRPQITTDFSI*RNNSDICGGIFL*W
DETRSHKARSYPERLSGQCESYQH*ATE*HPGD
S*IQEEV*L*DSVCCLMGSEFASGYREWPDAAGQ
KWPREGLSSYQPKTISTNGRT*GLECLGDNIWQ
KG*VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRR
GFGRMCTL*SCKI*KNQISGDCFEEFCGSLCVGT
KAISQIYGL*VIWRIGT*AITGGSHC*GRPEVE
SDLWILCWIPCC*CGFRISL*HLSTNTYPV*HQ
TPCNHHPPQYRWNGASGVL*R*GGLCKHIWKDHQ
GCSSTVGRDAYISSIYSIQSDNGLGREGHRDPI
CGNWSLGWCVHAQKGSKTKILV*TQ*QGVLCLC
SVWWQQSGLFHDLRQDFSSELVEAV*SRDYWPPE
SSRS*ELGIPCNWSSELHRGQPGQLCVQTSCVG
FSPLLPVPLIYQFIPILFFFLTPK*IKAAMQLV
LFRFYHQVL*VFGIEHHTGKQTPFLQLQNSLSLN
DSVLWVSDSGDDEHAVGFIGSGHKEVRSGGKRS
GVLKQRADLI**H*QCI*LTFLFCNVTICGQRR
RCRFKKLIFIKCERHSY*DNFFVGGAWEMGWGGL
RGPILFLWIWGGGPGQELSHFSVYQVA*IMCRW
F*KKKKKKKKKEKKKEKENVCILYNGQNFVV*Q
Y*HCLS*RFNFCLNLDVQQKFYHSLHLQKKEKNS
NHMFIFFLPTSLFLMH*EVI*FICFWKKPLMFN
LILIPKRPD*SLTFIVTNQQAQELSMKMGNSLK
ADAVYLQV*KPECFVFTRFTIRG*WGNCSP*HKI
SLFSM*RGW*QTGGYIRIVTQTVQCCRNLFLGV
GEFPFSKNAMH*NYFKNVVNSAYS*SGHLLCFR
CNIEVLAFLVFSLALLFSVFLNQFYFMNIFMTFV
INVLRKNLFHGFMVITQAPVRILPSQERIRTPC
HSPAILLSSCRVLSGNNCIMAALTSVIKRNLLF
FRLDLFPLWLIFLYIVKMGDFPSAPTHLNTAAIC
TCLLPIPLGTHSDLLSVSCSWFHLFEKGPPLSY
KHLVRQVHPLMNADTAAGGFVYTCKDKLRSLLF
GEVKEDGNGCFICMSLEQC*RPKPPTGL*LT*RR
LKN*SYL*RDLR*AGFHRHRTLVRIKFLLVIYI
HV*AFHKMKHATVNTLVYISREEGKAQVENSKR
WADVFEEMLNV*C*VGEGREQRMWHNGLNIIQRL
EAPCL*VKCYTKKNANGVSLELPSA*NLLAFP*
VVKGSELTPSCAWLACAAPGTRWALWWCLW*TE
LNPLLGLSY*NWNTLCPSRRGLPGLCFTWLFSFP
S*PHPLVNQIELL*LDAGTQ*SFSVKDWESSKC
LDSFRKGIIFPPFTG**LLHRSANMAKLHKKTV
LQ*HHYIRNIELLEECSSKQNKNVSRFSQSFHK*
*PFCIKIRVTLSVLSAVFFTLFSCTCYVGENVY
KNGFVTLMRTTYLWFILSDFLWVI*VFVLVVAH
LP*FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQ
VHSPFFCSANLSTVYYENYCCPPTLFSLNKVKM
TPILCGMSFEYVLTLQNVSFL*GSIFYAYLPTL
SFPYQSRLLYFAISDDRGLPKARYRLQK*QMFV*
RPQR*SGR*TSITRFLKTVWSKAGP*RNVTFLQ
F*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLDETEYEYSGSEEEEEEVPE
QEGEPSSIVNVPGESTLRRDFLRLQQENKERSE
ALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQ
QKEQRRRLEEQQRREREARRQQEREQRRREQEEK
RRLEELERRRKEEEERRRAEEEKRRVEREQEYI
RRQLEEEQRHLEVLQQQLLQEQAMLLECRWREM
EEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQH
SQQPPPPQQERSKPSFHAPEPKAHYEPADRARE
VPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRN
STSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGS
QPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE
NAVKKPEDKKEVFRPLKPAGEVDLTALAKELRA
VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEG
ADESTSGPEDTRAASSLNLSNGETESVKTMIVH
DDVESEPAMTPSKEGTLIVRQTQSASSTLQKHK
SSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDG
MRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEI
RKYKKRFNSEILCAALWGVNLLVGTESGLMLLD
RSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGK
KDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVG
DLEGCVHYKVVKYERIKFLVIALKSSVEVYAWA
PKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLK
VIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIK
PHAIIILPNTDGMELLVCYEDEGVYVNTYGRIT
KDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRS
VETGHLDGVFMHKRAQRLKFLCERNDKVFFASV
RSGGSSQVYFMTLGRTSLLSW*KQCDPGITGLQ
SLQDPENLEFLVTGARSCTEGNQDSCVCRPHVLG
SLPSFLFLLYTSLSPFFFFFLLQNKSRLQCSWC
CSDSTIRCYKCLGLSIILESKHLSSSSRIPCL*
MTLSCGCLTVATMNMPLVLLAVGTRR*EVVVKGA
EC*SREQI*YSNINSVFN*HFFFVM*RYVDKEE
DAGLRS*YL*NVKDTVTRITFLWVGLGRWGGVG
*GVPFCFFGFGVGVLAKNSVIFLCTRLPKSCADG
SKKKKKKKKKRKKKKKKKTCAFCIMARTLSCDS
ISTASVKGLIFV*T*TCNKSFTTVCTCRRKKKI
QTTCLFFFCLPHCS*CIER*FSLYVFGRNH*CLI
*S*YQNDQIEV*LLLSQISRHKNCP*RWEIALR
LMQFTYKFRNQNALFLPDSPLEVDGATAAHDTR
SHCSRCRGVGSRQVVTLE*SHKLFSVAGTFSWGW
GSFPFLKMQCTKTILRM*LILLIHKVGIFCVLG
VISKSWLFSFSHLLSCSLFF*TNFTL*IYS*HL
**MS*ERICFMASWSSLKLP*GYYRLRKGSGLHV
TVLPSYFPLVEF*VEITALWLL*PQSSKETCCF
LGLIFFLCG*FSCIL*KWGIFPLLPPT*TQQPF
VPVCFPSHLAPTLTSCQFPVPGSIFLKKALL*AT
NIW*DKYIHS*MQTQQLVVLCIPVKTS*EAYFL
GK*KKMEMDVSFV*VWSSAEGQSRLLVCS*PRE
G*KINPTFKGI*GRLDSIATGL*LELNSCL*FIS
MFRLFIR*NMPQ*THSCTYQEKKERHRWRTVKG
GQMSLKKCSMSDAKWEKAENKGCGIMVLTLSKD
LKLHVCKSNVTQKKMQMVFHWNYQVLRTCWLSHR
W*RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN
*IHCWA*VTEIGTPFVLLGGGFLVCALLGFFPS
RLSLTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV
*ILLGKELFSPLLQGSNFSTEVPIWQNYTRKQY
CNDTIT*GTLNC*RSALPNKTKMSLGLVRAFTS
NNLSVLKSE*PFLY*VQCFLLFSHAHVTLEKMFT
KMVLLH*CAPHIYGLF*VTFYGLFRFSS*L*HT
YPNFANY*FAK**YK*QTALNLLIIKAAKQTGG
KYTALFFAVLTCLLCIMKITVVPPPFFP*IK*K*
HLFYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*
ASRTRAGSCILLFQMTGACPRPGIDYKSSKCLS
KDLRDEVGDEPPSPGS*RQYGVKLVLKEMSHFCS
FEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLMKLSMSTVGVRKKRRKCL
NRKESQVPL*TCLVSLLFAEIS*DCSRRTRNVPR
LFGDNSYYRSNSSGSRKNIKGNCWQRDRSGLSS
RKNRGDG*KSNKGESGKLEGSRNVNSEGENKKK
RGV*RSWREGAKKKRRGDGQKKKRGELKENRSIS
GDS*KRSSGTWKSFSSSCSRSRPCYWSADGGRW
RSTGRQRGSRGSCNKNKHISCLYSMTIGGRTRS
TRSSRHHRSRKGASQASMLPSPKPTTSLLTERER
FL*EQHLAPLFCPVEIPHCRAVGSRIARQDRET
PPAVLSPGFCGREWRSWCPDLAVAAPQGPATQD
PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAWK
MQ*KNLKIKRKFSDPSSLLAKWI*PHWPKSFEQ
WKMYGHLTK*RTTPHPVRSRGRRMRRTTMWSRK
GLTSPPQDQRTPEQRHL*I*AMVKRNL*KP*LSM
MM*KVSRP*PHPRRAL*SSARLSPLVAHSRNTN
LPPPLHLL*TPDYYRFLHLAEQQ*HLWWDFPVM
G*DQKP*GKILPGKAQWSM*ILPTLGHRVTPRRF
VNTRRGLTLRFCVLPYGE*IC*WVQRVA*CCWT
EVAKGRSILLSTEDDFNKWTYLRA*MSW*QYLA
KRISYVSTICPG*EIKYFTMIQKLRRSRDGQP*G
IWKDVYIIKL*NMKESNFW*LL*RVLWKSMRGH
QSHITNLWPLSHLENWYISHYWWISLLRKARG*
K*SMDPVLDSMLLMWIQDQSMTFIYQHISSVASN
PMQSSSSPIQMEWSFWCAMKMRGFM*THMEGSP
RM*FYSGERCLHQ*HIFDPIRQWAGERRP*RSD
LWKLVTWMVCSCTKGLKD*NSCVNAMTRCSLPLF
GLVAAVRFIS*P*AGLLF*AGRSSVIQGLLASR
VFKILRTWNSL*LELGAAPRATRTAVCADLMCW
VLSPPSCSSYIPVYPHSFFFSYSKINQGCNAAGA
VQILPSGAISVWD*ASYWKANTFPPAPEFLVSE
*LCLVGV*QWRR*TCRWFYWQWAQGGEKWW*KE
RSAEAESRFNIVTLTVYLIDISFL*CDDMWTKKK
MQV*EVNIYKM*KTQLLG*LFCGWGLGDGVGWV
KGSHFVSLDLGWGSWPRTQSFFCVPGCLNHVQM
VLKKKKKKKKKGKKKRKRKRVHFV*WPELCRVTV
LALPQLKV*FLFKPRRATKVLPQSALAEERKKF
KPHVYFFFAYLIVLNALRGDLVYMFLEETINV*
FNLNTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G
*CSLLTSLETRMLCFYQIHH*RLMGQLQPMTQD
LIVLDVEGLVADRWLH*NSHTNCSVLQEPFLGG
GGVSLF*KCNALKLF*ECS*FCLFIKWASSVF*V
*YRSPGFSRFLTCSLVLCFFKPILLYEYIHDIC
NKCLEKEFVSWLHGHHSSSRKDITVSGKDQDSM
SQSCHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF
*A*SFSFVVNFPVYCENGGFSLCSHPPKHSSHL
YLFASHPTWHPL*PLVSFLFLVPSF*KRPSFEL
QTSGKTSTSTHECRHSSWWFCVYL*RQAEKLTFW
GSKRRWKWMFHLYEFGAVLKAKAAYWFVVNLEK
VEKLILPLKGFEVGWIPSPQDFS*N*IPACNLY
PCLGFS*DETCHSEHTRVHIKRRRKGTGGEQ*KV
GRCL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT
*SSMSVSQMLHKKKCKWCFIGITKCLELAGFPI
GGKGV*AHTELCLACLCSSRHPVGTLVVFVVN*I
ESIVGLKLLKLEHPLSFSAGASWSVLYLAFFLP
VLASPPCQPD*VAIA*CRDPVKFLR*RLGVVEM
FRFF*ERNYFPPFYRVVTSPQKCQYGKITQENSI
AMTPLHKEH*TVRGVLFQTKQKCL*V*SELSQV
ITFLY*NQSNPFCIECSVFYSFLMHMLRWRKCL
QKWFCYTNAHHIFMVYFK*LFMGYLGFRLSCSTL
TLILPIINLLNSNTNDKLH*IY*L*KLQSRLVA
STQPFFLQC*LVYCVL*KLLLSPHPFFLK*SKN
DTYFMWHEFRICSDPSECFLPVRIHILCIPAYPE
LPVPEQAPVFCYFR*QGLAQGQV*ITKVANVCL
KTSEMKWEMNLHHQVPEDSME*SWSLKKCHIFA
VLNLDQKI

Sequence MAP4K4.fAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCC
CACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGT
GCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGA
GTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGC
CTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGA
GGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTG
AATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAA
TCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCC
ATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTC
AATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGG
GAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACA
AATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTT
CACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAAT
TTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACA
TAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGA
TCAGTCTATGACATTTATCTACCAACACATGTAAGAAAGAACCCACACTCTATGATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATA
CAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGAT
GCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTG
TTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATT
TCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATT
CCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTA
TATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATA
AGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGT
GGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAA
TATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTAT
AAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATT
TGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAA
AAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAA
TTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCT
ACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATT
GAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAA
ACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGG
TAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAA
TGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGT
TTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTT
CATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTT
GTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTT
AATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGC
ACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCAC
TCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATG
TTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGG
GATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAAC
ATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAA
TGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGT
TACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGA
GTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTG
AAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAG
ATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTT
TCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACAT
TGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTA
ACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCA
CCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTG
CTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCT
AACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCG
AATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTA
TTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGA
ACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGSCENNISLPCSVPSRFP
TAGQWAAE*PGRTEKLHQQY*AQASVGESGEAG
AQTWQWQLLRVQQLRIPARVSPWVSEWLRGTLQ
SEIIIQV*RLSISAPGKCSEKT*R*KGSFQTPQA
CWRSGSDRTGQRASSSGRCTATSQSNGLLLIQ*
GVGDDG*GGRRCGAGRG*RVHLRTRGHQSSVIS
EFEQW*NGICENHDCP**CRK*AGHDPIQGGHSN
RPPDSVR**HTPETQIFLLLYTFYRPQITTDFS
I*RNNSDICGGIFL*WDETRSHKARSYPERLSG
QCESYQH*ATE*HPGDS*IQEEV*L*DSVCCLMG
SEFASGYREWPDAAGQKWPREGLSSYQPKTIST
NGRT*GLECLGDNIWQKG*VTCLLFVLVKK*NT
SQ*SRS*EEAGMDNRRGFGRMCTL*SCKI*KNQI
SGDCFEEFCGSLCVGTKAISQIYGL*VIWRIGT
*AITGGSHC*GRPEVESDLWILCWIPCC*CGFR
ISL*HLSTNTCKKEPTLYDPV*HQTPCNHHPPQY
RWNGASGVL*R*GGLCKHIWKDHQGCSSTVGRD
AYISSIYSIQSDNGLGREGHRDPICGNWSLGWC
VHAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGLF
HDLRQDFSSELVEAV*SRDYWPPESSRS*ELGI
PCNWSSELHRGQPGQLCVQTSCVGFSPLLPVPL
IYQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL*
VFGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDS
GDDEHAVGFIGSGHKEVRSGGKRSGVLKQRADL
I**H*QCI*LTFLFCNVTICGQRRRCRFKKLIFI
KCERHSY*DNFFVGGAWEMGWGGLRGPILFLWI
WGGGPGQELSHFSVYQVA*IMCRWF*KKKKKKK
KKEKKKEKENVCILYNGQNFVV*QY*HCLS*RFN
FCLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLP
TSLFLMH*EVI*FICFWKKPLMFNLILIPKRPD
*SLTFIVTNQQAQELSMKMGNSLKADAVYLQV*K
PECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW
*QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNA
MH*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFLV
FSLALLFSVFLNQFYFMNIFMTFVINVLRKNLF
HGFMVITQAPVRILPSQERIRTPCHSPAILLSS
CRVLSGNNCIMAALTSVIKRNLLFFRLDLFPLWL
IFLYIVKMGDFPSAPTHLNTAAICTCLLPIPLG
THSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHP
LMNADTAAGGFVYTCKDKLRSLLFGEVKEDGNGC
FICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*R
DLR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMK
HATVNTLVYISREEGKAQVENSKRWADVFEEMLN
V*C*VGEGREQRMWHNGLNIIQRLEAPCL*VKC
YTKKNANGVSLELPSA*NLLAFP*VVKGSELTP
SCAWLACAAPGTRWALWWCLW*TELNPLLGLSY*
NWNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQ
IELL*LDAGTQ*SFSVKDWESSKCLDSFRKGII
FPPFTG**LLHRSANMAKLHKKTVLQ*HHYIRNI
ELLEECSSKQNKNVSRFSQSFHK**PFCIKIRV
TLSVLSAVFFTLFSCTCYVGENVYKNGFVTLMR
TTYLWFILSDFLWVI*VFVLVVAHLP*FCQLLIC
*IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSA
NLSTVYYENYCCPPTLFSLNKVKMTPILCGMSF
EYVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLLY
FAISDDRGLPKARYRLQK*QMFV*RPQR*SGR*
TSITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVPVRTTSRSPVLSRRDS
PLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLV
PRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFR
VRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLK
PAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSE
ESGTTDEEDDDVEQEGADESTSGPEDTRAASSL
NLSNGETESVKTMIVHDDVESEPAMTPSKEGTL
IVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISP
SSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVV
NVNPTNTRPQSDTPEIRKYKKRFNSEILCAALW
GVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQ
MDVLEGLNVLVTISGKKDKLRVYYLSWLRNKIL
HNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIK
FLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVH
KPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSG
SVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPN
TDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEM
PTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGV
FMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY
FMTLGRTSLLSW*KQCDPGITGLQSLQDPENLEF
LVTGARSCTEGNQDSCVCRPHVLGSLPSFLFLL
YTSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCY
KCLGLSIILESKHLSSSSRIPCL*MTLSCGCLTV
ATMNMPLVLLAVGTRR*EVVVKGAEC*SREQI*
YSNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL
*NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFGF
GVGVLAKNSVIFLCTRLPKSCADGSKKKKKKKK
KRKKKKKKKTCAFCIMARTLSCDSISTASVKGL
IFV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFCL
PHCS*CIER*FSLYVFGRNH*CLI*S*YQNDQI
EV*LLLSQISRHKNCP*RWEIALRLMQFTYKFR
NQNALFLPDSPLEVDGATAAHDTRSHCSRCRGVG
SRQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQ
CTKTILRM*LILLIHKVGIFCVLGVISKSWLFS
FSHLLSCSLFF*TNFTL*IYS*HL**MS*ERICF
MASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPL
VEF*VEITALWLL*PQSSKETCCFLGLIFFLCG
*FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHLA
PTLTSCQFPVPGSIFLKKALL*ATNIW*DKYIH
S*MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMD
VSFV*VWSSAEGQSRLLVCS*PREG*KINPTFKG
I*GRLDSIATGL*LELNSCL*FISMFRLFIR*N
MPQ*THSCTYQEKKERHRWRTVKGGQMSLKKCS
MSDAKWEKAENKGCGIMVLTLSKDLKLHVCKSNV
TQKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHR
VVLGLLVQLQAPGGHSGGVCGELN*IHCWA*VT
EIGTPFVLLGGGFLVCALLGFFPSRLSLTPLSTR
LSCYSLMQGPSEVSPLKIGSRRNV*ILLGKELF
SPLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GT
LNC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE*
PFLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CA
PHIYGLF*VTFYGLFRFSS*L*HTYPNFANY*F
AK**YK*QTALNLLIIKAAKQTGGKYTALFFAVL
TCLLCIMKITVVPPPFFP*IK*K*HLFYVA*VS
NMF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSC
ILLFQMTGACPRPGIDYKSSKCLSKDLRDEVGDE
PPSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERFL*EQHLAPLFCPVEIP
HCRAVGSRIARQDRETPPAVLSPGFCGREWRSW
CPDLAVAAPQGPATQDPSPGLTLGLRVAPGNASE
*DHHPSLKALHLSAWKMQ*KNLKIKRKFSDPSS
LLAKWI*PHWPKSFEQWKMYGHLTK*RTTPHPV
RSRGRRMRRTTMWSRKGLTSPPQDQRTPEQRHL*
I*AMVKRNL*KP*LSMMM*KVSRP*PHPRRAL*
SSARLSPLVAHSRNTNLPPPLHLL*TPDYYRFL
HLAEQQ*HLWWDFPVMG*DQKP*GKILPGKAQWS
M*ILPTLGHRVTPRRFVNTRRGLTLRFCVLPYG
E*IC*WVQRVA*CCWTEVAKGRSILLSTEDDFN
KWTYLRA*MSW*QYLAKRISYVSTICPG*EIKYF
TMIQKLRRSRDGQP*GIWKDVYIIKL*NMKESN
FW*LL*RVLWKSMRGHQSHITNLWPLSHLENWY
ISHYWWISLLRKARG*K*SMDPVLDSMLLMWIQD
QSMTFIYQHM*ERTHTL*SSVASNPMQSSSSPI
QMEWSFWCAMKMRGFM*THMEGSPRM*FYSGER
CLHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMVC
SCTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFI
S*P*AGLLF*AGRSSVIQGLLASRVFKILRTWN
SL*LELGAAPRATRTAVCADLMCWVLSPPSCSSY
IPVYPHSFFFSYSKINQGCNAAGAVQILPSGAI
SVWD*ASYWKANTFPPAPEFLVSE*LCLVGV*Q
WRR*TCRWFYWQWAQGGEKWW*KERSAEAESRFN
IVTLTVYLIDISFL*CDDMWTKKKMQV*EVNIY
KM*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLD
LGWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKKK
KGKKKRKRKRVHFV*WPELCRVTVLALPQLKV*
FLFKPRRATKVLPQSALAEERKKFKPHVYFFFA
YLIVLNALRGDLVYMFLEETINV*FNLNTKTTRL
KFDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLE
TRMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGL
VADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KCN
ALKLF*ECS*FCLFIKWASSVF*V*YRSPGFSR
FLTCSLVLCFFKPILLYEYIHDICNKCLEKEFV
SWLHGHHSSSRKDITVSGKDQDSMSQSCHLTFLL
SSSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVV
NFPVYCENGGFSLCSHPPKHSSHLYLFASHPTW
HPL*PLVSFLFLVPSF*KRPSFELQTSGKTSTST
HECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWM
FHLYEFGAVLKAKAAYWFVVNLEKVEKLILPLK
GFEVGWIPSPQDFS*N*IPACNLYPCLGFS*DET
CHSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQ
CLMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQM
LHKKKCKWCFIGITKCLELAGFPIGGKGV*AHTE
LCLACLCSSRHPVGTLVVFVVN*IESIVGLKLL
KLEHPLSFSAGASWSVLYLAFFLPVLASPPCQP
D*VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNYF
PPFYRVVTSPQKCQYGKITQENSIAMTPLHKEH
*TVRGVLFQTKQKCL*V*SELSQVITFLY*NQS
NPFCIECSVFYSFLMHMLRWRKCLQKWFCYTNAH
HIFMVYFK*LFMGYLGFRLSCSTLTLILPIINL
LNSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC
*LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEFR
ICSDPSECFLPVRIHILCIPAYPELPVPEQAPV
FCYFR*QGLAQGQV*ITKVANVCLKTSEMKWEM
NLHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Sequence MAP4K4.cAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCC
CACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCC
CAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTG
AGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTG
CTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGA
GTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAAT
TTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCG
TCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATC
TAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAAT
GTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAG
TGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAAT
GGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCAC
AATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTC
TGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAA
GCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCA
GTCTATGACATTTATCTACCAACACATGTAAGAAAGAACCCACACTCTATGATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAG
ATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCC
TACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTC
ATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCA
TGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCT
TGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATAT
ACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGT
GTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGC
GACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATAT
AGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAA
ATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGG
GGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA
AGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTT
TTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACC
TCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAA
GTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACC
AGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAG
CAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGC
ACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTT
CTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCAT
GGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTC
GAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAAT
TTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACC
CACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCA
TGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTT
CATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGAT
TTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATG
CCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGT
CTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTAC
ACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTT
GTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAA
TTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATT
GAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCC
CCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACATTGA
ACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACC
CTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCA
CATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTA
AATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAAC
TTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAAT
ATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTT
TGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACC
TCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGSCENNISLPCSVPSRFP
TAGQWAAE*PGRTEKLHQY*AQASVGESGEAGA
QTWQWQLLRVQQLRIPARVSPWVSEWLRGTLQS
EIIIQV*RLSISAPGKCSEKT*R*KGSFQTPQAC
WRSGSDRTGQRASSSGRCTATSQSNGLLLIQ*G
VGDDG*GGRRCGAGRG*RVHLRTRGHQSSVISE
FEQW*NGICENHDCP**CRK*AGHDPIQGGHSNR
PPDSVR**HTPETQIFLLLYTFYRPQITTDFSI
*RNNSDICGGIFL*WDETRSHKARSYPERLSGQ
CESYQH*ATE*HPGDS*IQEEV*L*DSVCCLMGS
EFASGYREWPDAAGQKWPREGLSSYQPKTISTN
GRT*GLECLGDNIWQKG*VTCLLFVLVKK*NTS
Q*SRS*EEAGMDNRRGFGRMCTL*SCKI*KNQIS
GDCFEEFCGSLCVGTKAISQIYGL*VIWRIGT*
AITGGSHC*GRPEVESDLWILCWIPCC*CGFRI
SL*HLSTNTCKKEPTLYDPV*HQTPCNHHPPQYR
WNGASGVL*R*GGLCKHIWKDHQGCSSTVGRDA
YISSIYSIQSDNGLGREGHRDPICGNWSLGWCV
HAQKGSKTKILV*TQ*QGVLCLCSVWWQQSGLFH
DLRQDFSSELVEAV*SRDYWPPESSRS*ELGIP
CNWSSELHRGQPGQLCVQTSCVGFSPLLPVPLI
YQFIPILFFFLTPK*IKAAMQLVLFRFYHQVL*V
FGIEHHTGKQTPFLQLQNSLSLNDSVLWVSDSG
DDEHAVGFIGSGHKEVRSGGKRSGVLKQRADLI
**H*QCI*LTFLFCNVTICGQRRRCRFKKLIFIK
CERHSY*DNFFVGGAWEMGWGGLRGPILFLWIW
GGGPGQELSHFSVYQVA*IMCRWF*KKKKKKKK
KEKKKEKENVCILYNGQNFVV*QY*HCLS*RFNF
CLNLDVQQKFYHSLHLQKKEKNSNHMFIFFLPT
SLFLMH*EVI*FICFWKKPLMFNLILIPKRPD*
SLTFIVTNQQAQELSMKMGNSLKADAVYLQV*KP
ECFVFTRFTIRG*WGNCSP*HKISLFSM*RGW*
QTGGYIRIVTQTVQCCRNLFLGVGEFPFSKNAM
H*NYFKNVVNSAYS*SGHLLCFRCNIEVLAFLVF
SLALLFSVFLNQFYFMNIFMTFVINVLRKNLFH
GFMVITQAPVRILPSQERIRTPCHSPAILLSSC
RVLSGNNCIMAALTSVIKRNLLFFRLDLFPLWLI
FLYIVKMGDFPSAPTHLNTAAICTCLLPIPLGT
HSDLLSVSCSWFHLFEKGPPLSYKHLVRQVHPL
MNADTAAGGFVYTCKDKLRSLLFGEVKEDGNGCF
ICMSLEQC*RPKPPTGL*LT*RRLKN*SYL*RD
LR*AGFHRHRTLVRIKFLLVIYIHV*AFHKMKH
ATVNTLVYISREEGKAQVENSKRWADVFEEMLNV
*C*VGEGREQRMWHNGLNIIQRLEAPCL*VKCY
TKKNANGVSLELPSA*NLLAFP*VVKGSELTPS
CAWLACAAPGTRWALWWCLW*TELNPLLGLSY*N
WNTLCPSRRGLPGLCFTWLFSFPS*PHPLVNQI
ELL*LDAGTQ*SFSVKDWESSKCLDSFRKGIIF
PPFTG**LLHRSANMAKLHKKTVLQ*HHYIRNIE
LLEECSSKQNKNVSRFSQSFHK**PFCIKIRVT
LSVLSAVFFTLFSCTCYVGENVYKNGFVTLMRT
TYLWFILSDFLWVI*VFVLVVAHLP*FCQLLIC*
IVIQMTNCIKFTNYKSCKADWWQVHSPFFCSAN
LSTVYYENYCCPPTLFSLNKVKMTPILCGMSFE
YVLTLQNVSFL*GSIFYAYLPTLSFPYQSRLLYF
AISDDRGLPKARYRLQK*QMFV*RPQR*SGR*T
SITRFLKTVWSKAGP*RNVTFLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVPVRTTSRSPVLSRRDS
PLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVP
RPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRV
RSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKP
AGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEE
SGTTDEEDDDVEQEGADESTSGPEDTRAASSLN
LSNGETESVKTMIVHDDVESEPAMTPSKEGTLI
VRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPS
SGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVN
VNPTNTRPQSDTPEIRKYKKRFNSEILCAALWG
VNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQM
DVLEGLNVLVTISGKKDKLRVYYLSWLRNKILH
NDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKF
LVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHK
PLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGS
VYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNT
DGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMP
TSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVF
MHKRAQRLKFLCERNDKVFFASVRSGGSSQVYF
MTLGRTSLLSW*KQCDPGITGLQSLQDPENLEFL
VTGARSCTEGNQDSCVCRPHVLGSLPSFLFLLY
TSLSPFFFFFLLQNKSRLQCSWCCSDSTIRCYK
CLGLSIILESKHLSSSSRIPCL*MTLSCGCLTVA
TMNMPLVLLAVGTRR*EVVVKGAEC*SREQI*Y
SNINSVFN*HFFFVM*RYVDKEEDAGLRS*YL*
NVKDTVTRITFLWVGLGRWGGVG*GVPFCFFGFG
VGVLAKNSVIFLCTRLPKSCADGSKKKKKKKKK
RKKKKKKKTCAFCIMARTLSCDSISTASVKGLI
FV*T*TCNKSFTTVCTCRRKKKIQTTCLFFFCLP
HCS*CIER*FSLYVFGRNH*CLI*S*YQNDQIE
V*LLLSQISRHKNCP*RWEIALRLMQFTYKFRN
QNALFLPDSPLEVDGATAAHDTRSHCSRCRGVGS
RQVVTLE*SHKLFSVAGTFSWGWGSFPFLKMQC
TKTILRM*LILLIHKVGIFCVLGVISKSWLFSF
SHLLSCSLFF*TNFTL*IYS*HL**MS*ERICFM
ASWSSLKLP*GYYRLRKGSGLHVTVLPSYFPLV
EF*VEITALWLL*PQSSKETCCFLGLIFFLCG*
FSCIL*KWGIFPLLPPT*TQQPFVPVCFPSHLAP
TLTSCQFPVPGSIFLKKALL*ATNIW*DKYIHS
*MQTQQLVVLCIPVKTS*EAYFLGK*KKMEMDV
SFV*VWSSAEGQSRLLVCS*PREG*KINPTFKGI
*GRLDSIATGL*LELNSCL*FISMFRLFIR*NM
PQ*THSCTYQEKKERHRWRTVKGGQMSLKKCSM
SDAKWEKAENKGCGIMVLTLSKDLKLHVCKSNVT
QKKMQMVFHWNYQVLRTCWLSHRW*RGLSSHRV
VLGLLVQLQAPGGHSGGVCGELN*IHCWA*VTE
IGTPFVLLGGGFLVCALLGFFPSRLSLTPLSTRL
SCYSLMQGPSEVSPLKIGSRRNV*ILLGKELFS
PLLQGSNFSTEVPIWQNYTRKQYCNDTIT*GTL
NC*RSALPNKTKMSLGLVRAFTSNNLSVLKSE*P
FLY*VQCFLLFSHAHVTLEKMFTKMVLLH*CAP
HIYGLF*VTFYGLFRFSS*L*HTYPNFANY*FA
K**YK*QTALNLLIIKAAKQTGGKYTALFFAVLT
CLLCIMKITVVPPPFFP*IK*K*HLFYVA*VSN
MF*PFRMFPSCEDPYFMHTCLP*ASRTRAGSCI
LLFQMTGACPRPGIDYKSSKCLSKDLRDEVGDEP
PSPGS*RQYGVKLVLKEMSHFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERFL*EQHLAPLFCPVEIP
HCRAVGSRIARQDRETPPVLSPGFCGREWRSWC
PDLAVAAPQGPATQDPSPGLTLGLRVAPGNASE*
DHHPSLKALHLSAWKMQ*KNLKIKRKFSDPSSL
LAKWI*PHWPKSFEQWKMYGHLTK*RTTPHPVR
SRGRRMRRTTMWSRKGLTSPPQDQRTPEQRHL*I
*AMVKRNL*KP*LSMMM*KVSRP*PHPRRAL*S
SARLSPLVAHSRNTNLPPPLHLL*TPDYYRFLH
LAEQQ*HLWWDFPVMG*DQKP*GKILPGKAQWSM
*ILPTLGHRVTPRRFVNTRRGLTLRFCVLPYGE
*IC*WVQRVA*CCWTEVAKGRSILLSTEDDFNK
WTYLRA*MSW*QYLAKRISYVSTICPG*EIKYFT
MIQKLRRSRDGQP*GIWKDVYIIKL*NMKESNF
W*LL*RVLWKSMRGHQSHITNLWPLSHLENWYI
SHYWWISLLRKARG*K*SMDPVLDSMLLMWIQDQ
SMTFIYQHM*ERTHTL*SSVASNPMQSSSSPIQ
MEWSFWCAMKMRGFM*THMEGSPRM*FYSGERC
LHQ*HIFDPIRQWAGERRP*RSDLWKLVTWMVCS
CTKGLKD*NSCVNAMTRCSLPLFGLVAAVRFIS
*P*AGLLF*AGRSSVIQGLLASRVFKILRTWNS
L*LELGAAPRATRTAVCADLMCWVLSPPSCSSYI
PVYPHSFFFSYSKINQGCNAAGAVQILPSGAIS
VWD*ASYWKANTFPPAPEFLVSE*LCLVGV*QW
RR*TCRWFYWQWAQGGEKWW*KERSAEAESRFNI
VTLTVYLIDISFL*CDDMWTKKKMQV*EVNIYK
M*KTQLLG*LFCGWGLGDGVGWVKGSHFVSLDL
GWGSWPRTQSFFCVPGCLNHVQMVLKKKKKKKKK
GKKKRKRKRVHFV*WPELCRVTVLALPQLKV*F
LFKPRRATKVLPQSALAEERKKFKPHVYFFFAY
LIVLNALRGDLVYMFLEETINV*FNLNTKTTRLK
FDFYCHKSAGTRTVHEDGK*P*G*CSLLTSLET
RMLCFYQIHH*RLMGQLQPMTQDLIVLDVEGLV
ADRWLH*NSHTNCSVLQEPFLGGGGVSLF*KCNA
LKLF*ECS*FCLFIKWASSVF*V*YRSPGFSRF
LTCSLVLCFFKPILLYEYIHDICNKCLEKEFVS
WLHGHHSSSRKDITVSGKDQDSMSQSCHLTFLLS
SSEWK*LHYGCFNLSHQKKLAVF*A*SFSFVVN
FPVYCENGGFSLCSHPPKHSSHLYLFASHPTWH
PL*PLVSFLFLVPSF*KRPSFELQTSGKTSTSTH
ECRHSSWWFCVYL*RQAEKLTFWGSKRRWKWMF
HLYEFGAVLKAKAAYWFVVNLEKVEKLILPLKG
FEVGWIPSPQDFS*N*IPACNLYPCLGFS*DETC
HSEHTRVHIKRRRKGTGGEQ*KVGRCL*RNAQC
LMLSGRRQRTKDVA*WS*HYPKT*SSMSVSQML
HKKKCKWCFIGITKCLELAGFPIGGKGV*AHTEL
CLACLCSSRHPVGTLVVFVVN*IESIVGLKLLK
LEHPLSFSAGASWSVLYLAFFLPVLASPPCQPD
*VAIA*CRDPVKFLR*RLGVVEMFRFF*ERNYFP
PFYRVVTSPQKCQYGKITQENSIAMTPLHKEH*
TVRGVLFQTKQKCL*V*SELSQVITFLY*NQSN
PFCIECSVFYSFLMHMLRWRKCLQKWFCYTNAHH
IFMVYFK*LFMGYLGFRLSCSTLTLILPIINLL
NSNTNDKLH*IY*L*KLQSRLVASTQPFFLQC*
LVYCVL*KLLLSPHPFFLK*SKNDTYFMWHEFRI
CSDPSECFLPVRIHILCIPAYPELPVPEQAPVF
CYFR*QGLAQGQV*ITKVANVCLKTSEMKWEMN
LHHQVPEDSME*SWSLKKCHIFAVLNLDQKI

Sequence MAP4K4.lAug05

TCACCTCATTAGTGTCCACCTGGCACTGACTGATCAGCACACTATAACATCATGAGAAAGATGGCGGGAAGATCCCTGGTAATTATACATTTTTACTTAC
GTAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGA
AGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGC
GGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACC
ACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGG
AAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACT
CCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACT
GCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGA
CCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGAT
CATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGA
TCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

SPH*CPPGTD*SAHYNIMRKMAGRSLVIIHFYL
RSNKGESGKLEGSRNVNSEGENKKKRGV*RSWRE
GAKKKRRGDGQKKKRGELKENRSISGDS*KRSS
GTWKSFSSSCSRSRPCYCMTIGGRTRSTRSSRH
HRSRKGASQASMLPSPKPTTSLLTERERWKIDLG
KLTTAPLKPSLSRQAEYWSHQCLPDQSLFPMAT
PSLCIPPCRDQRSHRFL*EQHLAPLFCPVEIPH
CRAVGSRIARQDRETPPVLSPGFCGREWRSWCPD
LAVAAPQGPATQDPSPGLTLGLRVAPGNASE*D
HHPSLKALHLSAWKMQ*KNLKIKRKFSDPSSLL
I*PHWPKSFEQW

Frame 1

HLISVHLALTDQHTITS*ERWREDPW*LYIFTY
VATKERAGS*KAAGT*TAKERTRRKEASRGVGE
KAQRRRGEETGRRRKEES*KRTGVYQATARRGAA
ALGSPSAAAAPGAGHVTA*P*EAAPAALAAAAT
TAAGKEQAKLPCSRAQSPLRAC*PSARGGR*I*
EN*PQLP*SPV*ADRQSIGATSAFPIRVFFQWQL
RVCASRPAETSGATGSCENNISLPCSVPSRFPT
AGQWAAE*PGRTEKLHQY*AQASVGESGEAGAQ
TWQWQLLRVQQLRIPARVSPWVSEWLRGTLQSEI
IIQV*RLSISAPGKCSEKT*R*KGSFQTPQAC*
SDRTGQRASSSG

Frame 2

TSLVSTWH*LISTL*HHEKDGGKIPGNYTFLLT
*QQRREREARRQQEREQRRREQEEKRRLEELER
RRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQ
RHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPP
PQQERSKPSFHAPEPKAHYEPADRAREVEDRFR
KTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGN
SESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPL
QGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPR
PGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVR
SSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAD
LTALAKELRAV

Sequence MAP4K4.eAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA
AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC
GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA
CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA
ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA
GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAG
CTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGG
AGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCAT
GATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAG
AAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTT
CCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACAC
CCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATG
CTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAA
TATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGAC
AACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTAT
GCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCC
AGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTG
TAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGA
AGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAG
AGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTT
TGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACT
GGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTC
ATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATG
CAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCT
TGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGG
TAAAAGGAGCGGAGTGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGA
CAAAGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGT
GGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCAT
GTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTC
GTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAG
AAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATT
AATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAAT
AGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCAT
GACACAAGATCTCATTGTTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTT
CTTGGGGGTGGGGGAGTTTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTG
TGTTTTAGGTGTAATATCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCA
TGACATTTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAG
GACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAAC
TTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAG
CAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCC
TTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGC
TTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAA
CCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGT
AATTTATATCCATGTTTAGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAAC
AGTAAAAGGTGGGCAGATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTA
TCCAAAGACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGC
TTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGG
TGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTT
TTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTC
GTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAG
AAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCT
TTCACAAGTAATAACCTTTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGA
AAATGTTTACAAAAATGGTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTT
GTAGCACACTTACCCTAATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAG
ACTGGTGGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAA
AGTAAAAATGACACCTATTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTG
CCTACCCTGAGCTTCCCGTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAA
TGTTTGTCTAAAGACCTCAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCAC
ATTTTTGCAGTTTTGAATTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV*
ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS
GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR
TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ
LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS
APGKCSEKT*R*KGSFQTPQACWRSGSDRTGQR
ASSSGRCTATSQSNGLLLIQ*GVGDDG*GGRRCG
AGRG*RVHLRTRGHQSSVISEFEQW*NGICENH
DCP**CRK*AGHDPIQGGHSNRPPDSVR**HTP
ETQIFLLLYTFYRPQITTDFSI*RNNSDICGGIF
L*WDETRSHKARSYPERLSGQCESYQH*ATE*H
PGDS*IQEEV*L*DSVCCLMGSEFASGYREWPD
AAGQKWPREGLSSYQPKTISTNGRT*GLECLGDN
IWQKG*VTCLLFVLVKK*NTSQ*SRS*EEAGMD
NRRGFGRMCTL*SCKI*KNQISGDCFEEFCGSL
CVGTKAISQIYGL*VIWRIGT*AITGGSHC*GRP
EVESDLWILCWIPCC*CGFRISL*HLSTNTYPV
*HQTPCNHHPPQYRWNGASGVL*R*GGLCKHIW
KDHQGCSSTVGRDAYISSIYSIQSDNGLGREGHR
DPICGNWSLGWCVHAQKGSKTKILV*TQ*QGVL
CLCSVWWQQSGLFHDLRQDFSSELVEAV*SRDY
WPPESSRS*ELGIPCNWSSELHRGQPGQLCVQTS
CVGFSPLLPVPLIYQFIPILFFFLTPK*IKAAM
QLVLFRFYHQVL*VFGIEHHTGKQTPFLQLQNS
LSLNDSVLWVSDSGDDEHAVGFIGSGHKEVRSGG
KRSGVLKQRADLI**H*QCI*LTFLFCNVTICG
QRRRCRFKKLIFIKCERHSY*DNFFVGGAWEMG
WGGLRGPILFLWIWGGGPGQELSHFSVYQVA*IM
CRWF*KKKKKKKKKEKKKEKENVCILYNGQNFV
V*QY*HCLS*RFNFCLNLDVQQKFYHSLHLQKK
EKNSNHMFIFFLPTSLFLMH*EVI*FICFWKKPL
MFNLILIPKRPD*SLTFIVTNQQAQELSMKMGN
SLKADAVYLQV*KPECFVFTRFTIRG*WGNCSP
*HKISLFSM*RGW*QTGGYIRIVTQTVQCCRNLF
LGVGEFPFSKNAMH*NYFKNVVNSAYS*SGHLL
CFRCNIEVLAFLVFSLALLFSVFLNQFYFMNIF
MTFVINVLRKNLFHGFMVITQAPVRILPSQERIR
TPCHSPAILLSSCRVLSGNNCIMAALTSVIKRN
LLFFRLDLFPLWLIFLYIVKMGDFPSAPTHLNT
AAICTCLLPIPLGTHSDLLSVSCSWFHLFEKGPP
LSYKHLVRQVHPLMNADTAAGGFVYTCKDKLRS
LLFGEVKEDGNGCFICMSLEQC*RPKPPTGL*L
T*RRLKN*SYL*RDLR*AGFHRHRTLVRIKFLLV
IYIHV*AFHKMKHATVNTLVYISREEGKAQVEN
SKRWADVFEEMLNV*C*VGEGREQRMWHNGLNI
IQRLEAPCL*VKCYTKKNANGVSLELPSA*NLLA
FP*VVKGSELTPSCAWLACAAPGTRWALWWCLW
*TELNPLLGLSY*NWNTLCPSRRGLPGLCFTWL
FSFPS*PHPLVNQIELL*LDAGTQ*SFSVKDWES
SKCLDSFRKGIIFPPFTG**LLHRSANMAKLHK
KTVLQ*HHYIRNIELLEECSSKQNKNVSRFSQS
FHK**PFCIKIRVTLSVLSAVFFTLFSCTCYVGE
NVYKNGFVTLMRTTYLWFILSDFLWVI*VFVLV
VAHLP*FCQLLIC*IVIQMTNCIKFTNYKSCKA
DWWQVHSPFFCSANLSTVYYENYCCPPTLFSLNK
VKMTPILCGMSFEYVLTLQNVSFL*GSIFYAYL
PTLSFPYQSRLLYFAISDDRGLPKARYRLQK*Q
MFV*RPQR*SGR*TSITRFLKTVWSKAGP*RNVT
FLQF*IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS
KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA
EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG
QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS
NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ
RLENAVKKPEDKKEVFRPLKPAGEVDLTALAKE
LRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDV
EQEGADESTSGPEDTRAASSLNLSNGETESVKTM
IVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQ
KHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGF
SCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDT
PEIRKYKKRFNSEILCAALWGVNLLVGTESGLM
LLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVT
ISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWT
TVGDLEGCVHYKVVKYERIKFLVIALKSSVEVY
AWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEG
QRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQC
SIKPHAIIILPNTDGMELLVCYEDEGVYVNTYG
RITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAI
EIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFF
ASVRSGGSSQVYFMTLGRTSLLSW*KQCDPGIT
GLQSLQDPENLEFLVTGARSCTEGNQDSCVCRP
HVLGSLPSFLFLLYTSLSPFFFFFLLQNKSRLQC
SWCCSDSTIRCYKCLGLSIILESKHLSSSSRIP
CL*MTLSCGCLTVATMNMPLVLLAVGTRR*EVV
VKGAEC*SREQI*YSNINSVFN*HFFFVM*RYVD
KEEDAGLRS*YL*NVKDTVTRITFLWVGLGRWG
GVG*GVPFCFFGFGVGVLAKNSVIFLCTRLPKS
CADGSKKKKKKKKKRKKKKKKKTCAFCIMARTLS
CDSISTASVKGLIFV*T*TCNKSFTTVCTCRRK
KKIQTTCLFFFCLPHCS*CIER*FSLYVFGRNH
*CLI*S*YQNDQIEV*LLLSQISRHKNCP*RWEI
ALRLMQFTYKFRNQNALFLPDSPLEVDGATAAH
DTRSHCSRCRGVGSRQVVTLE*SHKLFSVAGTF
SWGWGSFPFLKMQCTKTILRM*LILLIHKVGIFC
VLGVISKSWLFSFSHLLSCSLFF*TNFTL*IYS
*HL**MS*ERICFMASWSSLKLP*GYYRLRKGS
GLHVTVLPSYFPLVEF*VEITALWLL*PQSSKET
CCFLGLIFFLCG*FSCIL*KWGIFPLLPPT*TQ
QPFVPVCFPSHLAPTLTSCQFPVPGSIFLKKAL
L*ATNIW*DKYIHS*MQTQQLVVLCIPVKTS*EA
YFLGK*KKMEMDVSFV*VWSSAEGQSRLLVCS*
PREG*KINPTFKGI*GRLDSIATGL*LELNSCL
*FISMFRLFIR*NMPQ*THSCTYQEKKERHRWRT
VKGGQMSLKKCSMSDAKWEKAENKGCGIMVLTL
SKDLKLHVCKSNVTQKKMQMVFHWNYQVLRTCW
LSHRW*RGLSSHRVVLGLLVQLQAPGGHSGGVCG
ELN*IHCWA*VTEIGTPFVLLGGGFLVCALLGF
FPSRLSLTPLSTRLSCYSLMQGPSEVSPLKIGS
RRNV*ILLGKELFSPLLQGSNFSTEVPIWQNYTR
KQYCNDTIT*GTLNC*RSALPNKTKMSLGLVRA
FTSNNLSVLKSE*PFLY*VQCFLLFSHAHVTLE
KMFTKMVLLH*CAPHIYGLF*VTFYGLFRFSS*L
*HTYPNFANY*FAK**YK*QTALNLLIIKAAKQ
TGGKYTALFFAVLTCLLCIMKITVVPPPFFP*I
K*K*HLFYVA*VSNMF*PFRMFPSCEDPYFMHTC
LP*ASRTRAGSCILLFQMTGACPRPGIDYKSSK
CLSKDLRDEVGDEPPSPGS*RQYGVKLVLKEMS
HFCSFEFGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL
SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ
RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD
RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA
TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL
SAWKMQ*KNLKIKRKFSDPSSLLAKWI*PHWPKS
FEQWKMYGHLTK*RTTPHPVRSRGRRMRRTTMW
SRKGLTSPPQDQRTPEQRHL*I*AMVKRNL*KP
*LSMMM*KVSRP*PHPRRAL*SSARLSPLVAHSR
NTNLPPPLHLL*TPDYYRFLHLAEQQ*HLWWDF
PVMG*DQKP*GKILPGKAQWSM*ILPTLGHRVT
PRRFVNTRRGLTLRFCVLPYGE*IC*WVQRVA*C
CWTEVAKGRSILLSTEDDFNKWTYLRA*MSW*Q
YLAKRISYVSTICPG*EIKYFTMIQKLRRSRDG
QP*GIWKDVYIIKL*NMKESNFW*LL*RVLWKSM
RGHQSHITNLWPLSHLENWYISHYWWISLLRKA
RG*K*SMDPVLDSMLLMWIQDQSMTFIYQHISS
VASNPMQSSSSPIQMEWSFWCAMKMRGFM*THME
GSPRM*FYSGERCLHQ*HIFDPIRQWAGERRP*
RSDLWKLVTWMVCSCTKGLKD*NSCVNAMTRCS
LPLFGLVAAVRFIS*P*AGLLF*AGRSSVIQGLL
ASRVFKILRTWNSL*LELGAAPRATRTAVCADL
MCWVLSPPSCSSYIPVYPHSFFFSYSKINQGCN
AAGAVQILPSGAISVWD*ASYWKANTFPPAPEFL
VSE*LCLVGV*QWRR*TCRWFYWQWAQGGEKWW
*KERSAEAESRFNIVTLTVYLIDISFL*CDDMW
TKKKMQV*EVNIYKM*KTQLLG*LFCGWGLGDGV
GWVKGSHFVSLDLGWGSWPRTQSFFCVPGCLNH
VQMVLKKKKKKKKKGKKKRKRKRVHFV*WPELC
RVTVLALPQLKV*FLFKPRRATKVLPQSALAEER
KKFKPHVYFFFAYLIVLNALRGDLVYMFLEETI
NV*FNLNTKTTRLKFDFYCHKSAGTRTVHEDGK
*P*G*CSLLTSLETRMLCFYQIHH*RLMGQLQPM
TQDLIVLDVEGLVADRWLH*NSHTNCSVLQEPF
LGGGGVSLF*KCNALKLF*ECS*FCLFIKWASS
VF*V*YRSPGFSRFLTCSLVLCFFKPILLYEYIH
DICNKCLEKEFVSWLHGHHSSSRKDITVSGKDQ
DSMSQSCHLTFLLSSSEWK*LHYGCFNLSHQKK
LAVF*A*SFSFVVNFPVYCENGGFSLCSHPPKHS
SHLYLFASHPTWHPL*PLVSFLFLVPSF*KRPS
FELQTSGKTSTSTHECRHSSWWFCVYL*RQAEK
LTFWGSKRRWKWMFHLYEFGAVLKAKAAYWFVVN
LEKVEKLILPLKGFEVGWIPSPQDFS*N*IPAC
NLYPCLGFS*DETCHSEHTRVHIKRRRKGTGGE
Q*KVGRCL*RNAQCLMLSGRRQRTKDVA*WS*HY
PKT*SSMSVSQMLHKKKCKWCFIGITKCLELAG
FPIGGKGV*AHTELCLACLCSSRHPVGTLVVFV
VN*IESIVGLKLLKLEHPLSFSAGASWSVLYLAF
FLPVLASPPCQPD*VAIA*CRDPVKFLR*RLGV
VEMFRFF*ERNYFPPFYRVVTSPQKCQYGKITQ
ENSIAMTPLHKEH*TVRGVLFQTKQKCL*V*SEL
SQVITFLY*NQSNPFCIECSVFYSFLMHMLRWR
KCLQKWFCYTNAHHIFMVYFK*LFMGYLGFRLS
CSTLTLILPIINLLNSNTNDKLH*IY*L*KLQSR
LVASTQPFFLQC*LVYCVL*KLLLSPHPFFLK*
SKNDTYFMWHEFRICSDPSECFLPVRIHILCIP
AYPELPVPEQAPVFCYFR*QGLAQGQV*ITKVAN
VCLKTSEMKWEMNLHHQVPEDSME*SWSLKKCH
IFAVLNLDQKI

Sequence MAP4K4.dAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTC
CTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCG
AGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGAC
AGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCA
CAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAA
ACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATC
CCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAA
AATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATG
TACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGA
GTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTA
GAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCT
CCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACC
AGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATAC
AAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCC
AAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAA
GTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAA
GGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCAT
ATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTA
TGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCA
ATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAG
TTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAAC
TGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGT
GGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAG
ATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCC
CTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGAT
TCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGT
CTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTG
AAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAGGT
TTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCC
CATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAA
AAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCACT
GCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACAT
GTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTA
ATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAG
TTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTT
CTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTT
CCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATATCG
AAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAATGT
CTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTCCT
GCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCTTG
ATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTT
GCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTG
GTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAA
AAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAA
TTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAG
GCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGATG
TCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCTCC
ATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAAAG
GGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCCAT
TGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCC
TCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCT
TTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGAC
ACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTT
CTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGT
TTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTAAT
TTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACACAG
CCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTATT
TTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCCGT
ACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCTCA
GAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAATT
TGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTGVPMAGDGGAPAGREAPEAVATRTSIS
PVSTA*P*EAAPAALAAAATTAAGKEQAKLPCSR
AQSPLRAC*PSARGGR*I*EN*PQLP*SPV*AD
RQSIGATSAFPIRVFFQWQLRVCASRPAETSGA
TGSCENNISLPCSVPSRFPTAGQWAAE*PGRTEK
LHQY*AQASVGESGEAGAQTWQWQLLRVQQLRI
PARVSPWVSEWLRGTLQSEIIIQV*RLSISAPG
KCSEKT*R*KGSFQTPQAC*SDRTGQRASSSGRC
TATSQSNGLLLIQ*GVGDDG*GGRRCGAGRG*R
VHLRTRGHQSSVISEFEQW*NGICENHDCP**C
RK*AGHDPIQGGHSNRPPDSVR**HTPETQIFLL
LYTFYRPQITTDFSI*RNNSDICGGIFL*WDET
RSHKARSYPERLSGQCESYQH*ATE*HPGDS*I
QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKWP
REGLSSYQPKTISTNGRT*GLECLGDNIWQKG*
VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG
RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKAI
SQIYGL*VIWRIGT*AITGGSHC*GRPEVESDL
WILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC
NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGCS
STVGRDAYISSIYSIQSDNGLGREGHRDPICGN
WSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW
WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESSR
S*ELGIPCNWSSELHRGQPGQLCVQTSCVGFSP
LLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR
FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDSV
LWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGVL
KQRADLI**H*QCI*LTFLFCNVTICGQRRRCR
FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRGP
ILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*K
KKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H
CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNHM
FIFFLPTSLFLMH*EVI*FICFWKKPLMFNLIL
IPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA
VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISLF
SM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGEF
PFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI
EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVINV
LRKNLFHGFMVITQAPVRILPSQERIRTPCHSP
AILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL
DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTCL
LPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKHL
VRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV
KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLKN
*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV*
AFHKMKHATVNTLVYISREEGKAQVENSKRWAD
VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEAP
CL*VKCYTKKNANGVSLELPSA*NLLAFP*VVK
GSELTPSCAWLACAAPGTRWALWWCLW*TELNP
LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*P
HPLVNQIELL*LDAGTQ*SFSVKDWESSKCLDS
FRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ*
HHYIRNIELLEECSSKQNKNVSRFSQSFHK**PF
CIKIRVTLSVLSAVFFTLFSCTCYVGENVYKNG
FVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP*
FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVHS
PFFCSANLSTVYYENYCCPPTLFSLNKVKMTPI
LCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP
YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RPQ
R*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*I
WIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLECRWREMEEHRQAERLQRQLQQEQAYL
LSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAP
EPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQT
GRVLEPPVPSRSESFSNGNSESVHPALQRPAEP
QVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQR
NSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGS
QPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLE
NAVKKPEDKKEVFRPLKPADLTALAKELRAVED
VRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADE
STSGPEDTRAASSLNLSNGETESVKTMIVHDDV
ESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSS
SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRP
EAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKY
KKRFNSEILCAALWGVNLLVGTESGLMLLDRSG
QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDK
LRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE
GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP
YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIY
GSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHA
IIILPNTDGMELLVCYEDEGVYVNTYGRITKDV
VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVET
GHLDGVFMHKRAQRLKFLCERNDKVFFASVRSG
GSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ
DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSLP
SFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCSD
STIRCYKCLGLSIILESKHLSSSSRIPCL*MTL
SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC*
SREQI*YSNINSVFN*HFFFVM*RYVDKEEDAG
LRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV
PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSKK
KKKKKKKRKKKKKKKTCAFCIMARTLSCDSIST
ASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT
CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S*
YQNDQIEV*LLLSQISRHKNCP*RWEIALRLMQ
FTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC
SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGSF
PFLKMQCTKTILRM*LILLIHKVGIFCVLGVIS
KSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M
S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTVL
PSYFPLVEF*VEITALWLL*PQSSKETCCFLGL
IFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV
CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNIW
*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK*
KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K
INPTFKGI*GRLDSIATGL*LELNSCL*FISMFR
LFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQM
SLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL
HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*R
GLSSHRVVLGLLVQLQAPGGHSGGVCGELN*IH
CWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS
LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*IL
LGKELFSPLLQGSNFSTEVPIWQNYTRKQYCND
TIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL
SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKMV
LLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYPN
FANY*FAK**YK*QTALNLLIIKAAKQTGGKYT
ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HLF
YVA*VSNMF*PFRMFPSCEDPYFMHTCLP*ASR
TRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL
RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFEF
GSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYWSADGGRWRSTGRQRGSRGSCNKNKHIS
CLYSMTIGGRTRSTRSSRHHRSRKGASQASMLP
SPKPTTSLLTERERWKIDLGKLTTAPLKPSLSR
QAEYWSHQCLPDQSLFPMATPSLCIPPCRDQRSH
RFL*EQHLAPLFCPVEIPHCRAVGSRIARQDRE
TPPVLSPGFCGREWRSWCPDLAVAAPQGPATQD
PSPGLTLGLRVAPGNASE*DHHPSLKALHLSAWK
MQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQWKM
YGHLTK*RTTPHPVRSRGRRMRRTTMWSRKGLT
SPPQDQRTPEQRHL*I*AMVKRNL*KP*LSMMM*
KVSRP*PHPRRAL*SSARLSPLVAHSRNTNLPP
PLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D
QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVNT
RRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEVA
KGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI
SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIWK
DVYIIKL*NMKESNFW*LL*RVLWKSMRGHQSH
ITNLWPLSHLENWYISHYWWISLLRKARG*K*S
MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPMQ
SSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM*
FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK
LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGLV
AAVRFIS*P*AGLLF*AGRSSVIQGLLASRVFK
ILRTWNSL*LELGAAPRATRTAVCADLMCWVLS
PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQI
LPSGAISVWD*ASYWKANTFPPAPEFLVSE*LC
LVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA
EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQV
*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKGS
HFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK
KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLAL
PQLKV*FLFKPRRATKVLPQSALAEERKKFKPH
VYFFFAYLIVLNALRGDLVYMFLEETINV*FNL
NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*CS
LLTSLETRMLCFYQIHH*RLMGQLQPMTQDLIV
LDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV
SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*YR
SPGFSRFLTCSLVLCFFKPILLYEYIHDICNKC
LEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS
CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A*
SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYLF
ASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS
GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGSK
RRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVEK
LILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL
GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGRC
L*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*SS
MSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK
GV*AHTELCLACLCSSRHPVGTLVVFVVN*IESI
VGLKLLKLEHPLSFSAGASWSVLYLAFFLPVLA
SPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF
F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAMT
PLHKEH*TVRGVLFQTKQKCL*V*SELSQVITF
LY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW
FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTLI
LPIINLLNSNTNDKLH*IY*L*KLQSRLVASTQ
PFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY
FMWHEFRICSDPSECFLPVRIHILCIPAYPELPV
PEQAPVFCYFR*QGLAQGQV*ITKVANVCLKTS
EMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN
LDQKI

Sequence MAP4K4.aAug05

GGGCCCGTCCTCGAGGCGCGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACGGCCGGCCCCGCGCCATGGTGTG
AGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCGCGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCGCC
CGCGGCCCCGCGAGGAGAGTACCGGGCCGGCTCGGCTGCCGCGCGAGGAGCGCGGTCGGCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACA
TTTATTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCT
GCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAAAG
TTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGC
TTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAA
GGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCA
AGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTT
CATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATT
ACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGT
CAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTT
TATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTAT
GAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAG
ATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAA
AAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGG
CAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAG
AAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCA
GGAGCAGGCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTA
AGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGC
GGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGA
CAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCA
ACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCA
GCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCA
GTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAG
GGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCA
TGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGCCAGCCATCATGAG
AGCAATGGCTTTGCCGGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCTCCACCTCCTCCTCCCCATCCTCCAGCC
AGCCGACACCCACCATGTCCCCACAGACACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTC
CTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGA
CCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAAT
ACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGG
CCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGAT
AAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGG
AAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCC
ATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATC
TATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATG
CAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGT
AGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAA
ACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTG
GTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCA
AGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTC
CCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAG
ATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCT
GTCTTGTGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGC
TGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCAG
GTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGT
CCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAATCATGTGCAGATGGTTCTAAA
AAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATGGCCAGAACTTTGTCGTGTGACAGTATTAGCA
CTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACCACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCAC
ATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGGTGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCT
TAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTGTCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGC
AGTTTACTTACAAGTTTAGAAACCAGAATGCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTG
TTCTCGATGTAGAGGGGTTGGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGT
TTCCCTTTTCTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATAT
CGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACATTTGTAATAAAT
GTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGATCAGGACTCCATGTCACAGTC
CTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAGTCATCAAAAGAAACTTGCTGTTTTTTAGGCT
TGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCTCTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGT
TTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCTGTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATC
TGGTAAGACAAGTACATCCACTCATGAATGCAGACACAGCAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGT
AAAAGAAGATGGAAATGGATGTTTCATTTGTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAA
AATTAATCCTACCTTTAAAGGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTT
AGGCTTTTCATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAGA
TGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAAGACTTGAAGCT
CCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGCTGGCTTTCCCATAGGTGGTAA
AGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCTGGTGGTGTTTGTGGTGAACTGAATTGAATCC
ATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTCCTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAG
CCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGACCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATT
CTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGTAGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATG
ACACCATTACATAAGGAACATTGAACTGTTAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCT
TTCTGTATTAAAATCAGAGTAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATG
GTTTTGTTACACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCTA
ATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGTGGCAAGTACAC
AGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAAATAAAGTAAAAATGACACCTA
TTTTATGTGGCATGAGTTTCGAATATGTTCTGACCCTTCAGAATGTTTCCTTCCTGTGAGGATCCATATTTTATGCATACCTGCCTACCCTGAGCTTCCC
GTACCAGAGCAGGCTCCTGTATTTTGCTATTTCAGATGACAGGGGCTTGCCCAAGGCCAGGTATAGATTACAAAAGTAGCAAATGTTTGTCTAAAGACCT
CAGAGATGAAGTGGGAGATGAACCTCCATCACCAGGTTCCTGAAGACAGTATGGAGTAAAGCTGGTCCTTAAAGAAATGTCACATTTTTGCAGTTTTGAA
TTTGGATCAAAAAATA
  • MAP4K4-F1 (forward primer)
  • MAP4K4-R1 (reverse primer)
  • Single exon skipping

Frame 0

GPVLEARGAGRGRRGLRRRATPGGLTAGPAPWC
ERRRPCTLRPPSARPGRQRAPSGPRAQPSPPPPP
AAPRGEYRAGSAAARGARSAAWSAAEIHRATET
FIVICFLVAKRENGERLPCKKSGGHRPLLPAGS
CWDF*AGGSGWKWHLWTSL*GSTC*NGSVGSHQS
YGCH*G*RGRNQTGDKYAKEILSSQKHCNILWC
FHQKEPSRT**PTLACYGVLWGWVHYRPCEEHQ
REHTQRRLDRLHLQRNPEGTGTSSHSSCDSPGYQ
GPECVAD*ECRGETC*LWCECSAGQDCGAEKYV
HRHSLLDGS*GHRL**EPRCHL*LQK*SLVLWH
YSH*DGRRCSPSL*HASNESTVSHSQKPSSPAEV
KKMVEEVF*FYRRVPGEELHAAALYRAAFETSF
YKGSAK*KAS*NPA*GSYRSYQEEERRER*N*V
*VQWE*GRRGGSA*TGRRAKFHCERAW*VYSSPR
FPETAAGEQGTFRGSSETTVTTGATAPGAGRI*
KATAGRETEAD*AAERTEATARRATKERAGS*K
AAGT*TAKERTRRKEASRGVGEKAQRRRGEETGR
RRKEES*KRTGVYQATARRGAAALGSPSAAAAP
GAGHVTA*P*EAAPAALAAAATTAAGKEQAKLP
CSRAQSPLRAC*PSARGGR*I*EN*PQLP*SPV*
ADRQSIGATSAFPIRVFFQWQLRVCASRPAETS
GATGSCENNISLPCSVPSRFPTAGQWAAE*PGR
TEKLHQQY*AQASVGESGEAGAQTWQWQLLRVQQ
LRIPARVSPWVSEWLRGTLQSEIIIQV*RLSIS
APGKCSEKT*R*KGSFQTPQAC*SDRTGQRASS
SGRCTATSQSNGLLLIQ*GVGDDG*GGRRCGAGR
G*RVHLRTRGHQSSVISEFEQW*NGICENHDCP
**CRK*AGHDPIQGGHSNRPPEYS*PKACQPS*
EQWLCRSHSPLARSLTAKPFLLHFLHLLLPILQP
ADTHHVPTDTPGQAHC**DSVR**HTPETQIFL
LLYTFYRPQITTDFSI*RNNSDICGGIFL*WDE
TRSHKARSYPERLSGQCESYQH*ATE*HPGDS*I
QEEV*L*DSVCCLMGSEFASGYREWPDAAGQKW
PREGLSSYQPKTISTNGRT*GLECLGDNIWQKG
*VTCLLFVLVKK*NTSQ*SRS*EEAGMDNRRGFG
RMCTL*SCKI*KNQISGDCFEEFCGSLCVGTKA
ISQIYGL*VIWRIGT*AITGGSHC*GRPEVESD
LWILCWIPCC*CGFRISL*HLSTNTYPV*HQTPC
NHHPPQYRWNGASGVL*R*GGLCKHIWKDHQGC
SSTVGRDAYISSIYSIQSDNGLGREGHRDPICG
NWSLGWCVHAQKGSKTKILV*TQ*QGVLCLCSVW
WQQSGLFHDLRQDFSSELVEAV*SRDYWPPESS
RS*ELGIPCNWSSELHRGQPGQLCVQTSCVGFS
PLLPVPLIYQFIPILFFFLTPK*IKAAMQLVLFR
FYHQVL*VFGIEHHTGKQTPFLQLQNSLSLNDS
VLWVSDSGDDEHAVGFIGSGHKEVRSGGKRSGV
LKQRADLI**H*QCI*LTFLFCNVTICGQRRRCR
FKKLIFIKCERHSY*DNFFVGGAWEMGWGGLRG
PILFLWIWGGGPGQELSHFSVYQVA*IMCRWF*
KKKKKKKKKEKKKEKENVCILYNGQNFVV*QY*H
CLS*RFNFCLNLDVQQKFYHSLHLQKKEKNSNH
MFIFFLPTSLFLMH*EVI*FICFWKKPLMFNLI
LIPKRPD*SLTFIVTNQQAQELSMKMGNSLKADA
VYLQV*KPECFVFTRFTIRG*WGNCSP*HKISL
FSM*RGW*QTGGYIRIVTQTVQCCRNLFLGVGE
FPFSKNAMH*NYFKNVVNSAYS*SGHLLCFRCNI
EVLAFLVFSLALLFSVFLNQFYFMNIFMTFVIN
VLRKNLFHGFMVITQAPVRILPSQERIRTPCHS
PAILLSSCRVLSGNNCIMAALTSVIKRNLLFFRL
DLFPLWLIFLYIVKMGDFPSAPTHLNTAAICTC
LLPIPLGTHSDLLSVSCSWFHLFEKGPPLSYKH
LVRQVHPLMNADTAAGGFVYTCKDKLRSLLFGEV
KEDGNGCFICMSLEQC*RPKPPTGL*LT*RRLK
N*SYL*RDLR*AGFHRHRTLVRIKFLLVIYIHV
*AFHKMKHATVNTLVYISREEGKAQVENSKRWAD
VFEEMLNV*C*VGEGREQRMWHNGLNIIQRLEA
PCL*VKCYTKKNANGVSLELPSA*NLLAFP*VV
KGSELTPSCAWLACAAPGTRWALWWCLW*TELNP
LLGLSY*NWNTLCPSRRGLPGLCFTWLFSFPS*
PHPLVNQIELL*LDAGTQ*SFSVKDWESSKCLD
SFRKGIIFPPFTG**LLHRSANMAKLHKKTVLQ*
HHYIRNIELLEECSSKQNKNVSRFSQSFHK**P
FCIKIRVTLSVLSAVFFTLFSCTCYVGENVYKN
GFVTLMRTTYLWFILSDFLWVI*VFVLVVAHLP*
FCQLLIC*IVIQMTNCIKFTNYKSCKADWWQVH
SPFFCSANLSTVYYENYCCPPTLFSLNKVKMTP
ILCGMSFEYVLTLQNVSFL*GSIFYAYLPTLSFP
YQSRLLYFAISDDRGLPKARYRLQK*QMFV*RP
QR*SGR*TSITRFLKTVWSKAGP*RNVTFLQF*
IWIKK

Frame 1

GPSSRRAARGAGAGA*GGGRRPGA*RPAPRHGV
SAAARARSVRPPRGPAGREPRAARERSRARRRR
PRPREESTGPARLPREERGRRPGLRLRYTERQRH
LLLFVFWWQKGKMANDSPAKSLVDIDLSSLRDP
AGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIK
VMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGA
FIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTK
GNTLKEDWIAYISREILRGLAHLHIHHVIHRDI
KGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTF
IGTPYWMAPEVIACDENPDATYDYRSDLWSCGI
TAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLK
SKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPF
IRDQPNERQVRIQLKDHIDRTRKKRGEKDETEY
EYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRR
DFLRLQQENKERSEALRRQQLLQEQQLREQEEYK
RQLLAERQKRIEQQKEQRRRLEEQQRREREARR
QQEREQRRREQEEKRRLEELERRRKEEEERRRA
EEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQ
EQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFH
APEPKAHYEPADRAREVEDRFRKTNHSSPEAQS
KQTGRVLEPPVPSRSESFSNGNSESVHPALQRPA
EPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAG
QRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSS
NSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQ
RLENAVKKPEDKKEVFRPLKPADLTALAKELRA
VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQE
GADESTSGPEDTRAASSLNLSNGETESVKTMIVH
DDVESEPAMTPSKEGTLIVRQSTVDQKRASHHE
SNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSS
QPTPTMSPQTPQDKLTANETQSASSTLQKHKSSS
SFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMR
PEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRK
YKKRFNSEILCAALWGVNLLVGTESGLMLLDRSG
QGKVYPLINRRRFQQMDVLEGLNVLVTISGKKD
KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDL
EGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKP
YHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI
YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH
AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDV
VLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVE
TGHLDGVFMHKRAQRLKFLCERNDKVFFASVRS
GGSSQVYFMTLGRTSLLSW*KQCDPGITGLQSLQ
DPENLEFLVTGARSCTEGNQDSCVCRPHVLGSL
PSFLFLLYTSLSPFFFFFLLQNKSRLQCSWCCS
DSTIRCYKCLGLSIILESKHLSSSSRIPCL*MTL
SCGCLTVATMNMPLVLLAVGTRR*EVVVKGAEC
*SREQI*YSNINSVFN*HFFFVM*RYVDKEEDA
GLRS*YL*NVKDTVTRITFLWVGLGRWGGVG*GV
PFCFFGFGVGVLAKNSVIFLCTRLPKSCADGSK
KKKKKKKKRKKKKKKKTCAFCIMARTLSCDSIS
TASVKGLIFV*T*TCNKSFTTVCTCRRKKKIQTT
CLFFFCLPHCS*CIER*FSLYVFGRNH*CLI*S
*YQNDQIEV*LLLSQISRHKNCP*RWEIALRLM
QFTYKFRNQNALFLPDSPLEVDGATAAHDTRSHC
SRCRGVGSRQVVTLE*SHKLFSVAGTFSWGWGS
FPFLKMQCTKTILRM*LILLIHKVGIFCVLGVI
SKSWLFSFSHLLSCSLFF*TNFTL*IYS*HL**M
S*ERICFMASWSSLKLP*GYYRLRKGSGLHVTV
LPSYFPLVEF*VEITALWLL*PQSSKETCCFLG
LIFFLCG*FSCIL*KWGIFPLLPPT*TQQPFVPV
CFPSHLAPTLTSCQFPVPGSIFLKKALL*ATNI
W*DKYIHS*MQTQQLVVLCIPVKTS*EAYFLGK
*KKMEMDVSFV*VWSSAEGQSRLLVCS*PREG*K
INPTFKGI*GRLDSIATGL*LELNSCL*FISMF
RLFIR*NMPQ*THSCTYQEKKERHRWRTVKGGQ
MSLKKCSMSDAKWEKAENKGCGIMVLTLSKDLKL
HVCKSNVTQKKMQMVFHWNYQVLRTCWLSHRW*
RGLSSHRVVLGLLVQLQAPGGHSGGVCGELN*I
HCWA*VTEIGTPFVLLGGGFLVCALLGFFPSRLS
LTPLSTRLSCYSLMQGPSEVSPLKIGSRRNV*I
LLGKELFSPLLQGSNFSTEVPIWQNYTRKQYCN
DTIT*GTLNC*RSALPNKTKMSLGLVRAFTSNNL
SVLKSE*PFLY*VQCFLLFSHAHVTLEKMFTKM
VLLH*CAPHIYGLF*VTFYGLFRFSS*L*HTYP
NFANY*FAK**YK*QTALNLLIIKAAKQTGGKYT
ALFFAVLTCLLCIMKITVVPPPFFP*IK*K*HL
FYVA*VSNMF*PFRMFPSCEDPYFMHTCLP*AS
RTRAGSCILLFQMTGACPRPGIDYKSSKCLSKDL
RDEVGDEPPSPGS*RQYGVKLVLKEMSHFCSFE
FGSKN

Frame 2

ARPRGARRGARAPGPEAAGDARGPDGRPRAMV*
APPPVHAPSALRAARPAESPERPESAAEPAAAA
RGPARRVPGRLGCRARSAVGGLVCG*DTQSDRD
IYCYLFFGGKKGKWRTTPLQKVWWTSTSPPCGIL
LGFLSWWKWLEMAPMDKSIRVDMLKRVSWQPSK
LWMSLRMKRKKSNWR*IC*RNTLITETLQHIMV
LSSKRALQDMMTNSGLLWSSVGLGPLQTL*RTPK
GTHSKKTGSLTSPEKS*GDWHIFTFIM*FTGIS
RARMCC*LRMQR*NLLTLV*VLSWTGLWGGEIR
S*ALPTGWLLRSSPVMRTQMPPMITEVIFGLVAL
QPLRWQKVLPLSVTCIQ*EHCFSFPETLLPG*S
QKNGRRSFLVL*KGAW*RITCSGPLQSSF*NIL
L*GISQMKGKLESSLRII*IVPGRREARKMKLSM
STVGVRKKRRKCLNRKESQVPL*TCLVSLLFAE
IS*DCSRRTRNVPRLFGDNSYYRSNSSGSRKNI
KGNCWQRDRSGLSSRKNRGDG*KSNKGESGKLEG
SRNVNSEGENKKKRGV*RSWREGAKKKRRGDGQ
KKKRGELKENRSISGDS*KRSSGTWKSFSSSCS
RSRPCYCMTIGGRTRSTRSSRHHRSRKGASQASM
LPSPKPTTSLLTERERWKIDLGKLTTAPLKPSL
SRQAEYWSHQCLPDQSLFPMATPSLCIPPCRDQ
RSHRFL*EQHLAPLFCPVEIPHCRAVGSRIARQD
RETPPAVLSPGFCGREWRSWCPDLAVAAPQGPA
TQDPSPGLTLGLRVAPGNASE*DHHPSLKALHL
SAWKMQ*KNLKIKRKFSDPSSLLI*PHWPKSFEQ
WKMYGHLTK*RTTPHPVRSRGRRMRRTTMWSRK
GLTSPPQDQRTPEQRHL*I*AMVKRNL*KP*LS
MMM*KVSRP*PHPRRAL*SSARVQLTKSVPAIMR
AMALPVAFTSCQISYSKAIPPPLPPPPPPHPPA
SRHPPCPHRHPRTSSLLMRLSPLVAHSRNTNLP
PPLHLL*TPDYYRFLHLAEQQ*HLWWDFPVMG*D
QKP*GKILPGKAQWSM*ILPTLGHRVTPRRFVN
TRRGLTLRFCVLPYGE*IC*WVQRVA*CCWTEV
AKGRSILLSTEDDFNKWTYLRA*MSW*QYLAKRI
SYVSTICPG*EIKYFTMIQKLRRSRDGQP*GIW
KDVYIIKL*NMKESNFW*LL*RVLWKSMRGHQS
HITNLWPLSHLENWYISHYWWISLLRKARG*K*S
MDPVLDSMLLMWIQDQSMTFIYQHISSVASNPM
QSSSSPIQMEWSFWCAMKMRGFM*THMEGSPRM
*FYSGERCLHQ*HIFDPIRQWAGERRP*RSDLWK
LVTWMVCSCTKGLKD*NSCVNAMTRCSLPLFGL
VAAVRFIS*P*AGLLF*AGRSSVIQGLLASRVF
KILRTWNSL*LELGAAPRATRTAVCADLMCWVLS
PPSCSSYIPVYPHSFFFSYSKINQGCNAAGAVQ
ILPSGAISVWD*ASYWKANTFPPAPEFLVSE*L
CLVGV*QWRR*TCRWFYWQWAQGGEKWW*KERSA
EAESRFNIVTLTVYLIDISFL*CDDMWTKKKMQ
V*EVNIYKM*KTQLLG*LFCGWGLGDGVGWVKG
SHFVSLDLGWGSWPRTQSFFCVPGCLNHVQMVLK
KKKKKKKKGKKKRKRKRVHFV*WPELCRVTVLA
LPQLKV*FLFKPRRATKVLPQSALAEERKKFKP
HVYFFFAYLIVLNALRGDLVYMFLEETINV*FNL
NTKTTRLKFDFYCHKSAGTRTVHEDGK*P*G*C
SLLTSLETRMLCFYQIHH*RLMGQLQPMTQDLI
VLDVEGLVADRWLH*NSHTNCSVLQEPFLGGGGV
SLF*KCNALKLF*ECS*FCLFIKWASSVF*V*Y
RSPGFSRFLTCSLVLCFFKPILLYEYIHDICNK
CLEKEFVSWLHGHHSSSRKDITVSGKDQDSMSQS
CHLTFLLSSSEWK*LHYGCFNLSHQKKLAVF*A
*SFSFVVNFPVYCENGGFSLCSHPPKHSSHLYL
FASHPTWHPL*PLVSFLFLVPSF*KRPSFELQTS
GKTSTSTHECRHSSWWFCVYL*RQAEKLTFWGS
KRRWKWMFHLYEFGAVLKAKAAYWFVVNLEKVE
KLILPLKGFEVGWIPSPQDFS*N*IPACNLYPCL
GFS*DETCHSEHTRVHIKRRRKGTGGEQ*KVGR
CL*RNAQCLMLSGRRQRTKDVA*WS*HYPKT*S
SMSVSQMLHKKKCKWCFIGITKCLELAGFPIGGK
GV*AHTELCLACLCSSRHPVGTLVVFVVN*IES
IVGLKLLKLEHPLSFSAGASWSVLYLAFFLPVL
ASPPCQPD*VAIA*CRDPVKFLR*RLGVVEMFRF
F*ERNYFPPFYRVVTSPQKCQYGKITQENSIAM
TPLHKEH*TVRGVLFQTKQKCL*V*SELSQVIT
FLY*NQSNPFCIECSVFYSFLMHMLRWRKCLQKW
FCYTNAHHIFMVYFK*LFMGYLGFRLSCSTLTL
ILPIINLLNSNTNDKLH*IY*L*KLQSRLVAST
QPFFLQC*LVYCVL*KLLLSPHPFFLK*SKNDTY
FMWHEFRICSDPSECFLPVRIHILCIPAYPELP
VPEQAPVFCYFR*QGLAQGQV*ITKVANVCLKT
SEMKWEMNLHHQVPEDSME*SWSLKKCHIFAVLN
LDQKI

Copyright ©2008,2017, all rights reserved.