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Sequences

Sequence LRDD.bAug05

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG
CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT
CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT
GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT
CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTG
GTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCT
CTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTG
CCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCC
TGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGC
CTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCC
TTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGA
CCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCC
CATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCAT
GGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACA
GCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCG
CCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAG
GAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCC
TGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCA
CCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACAC
TTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG
CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG
CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG
GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTCAGGCTGTGCGGGGCC
AGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCT
GCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTT
CTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCA
TCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCT
GGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGC
ATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCC
AGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACC
TCAGAGCTTGATTTATTTGTTGGATG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP
ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF
RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP
VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA
SEPVLPPLPGPQRRATPGHTGCLSPGCPDQPARW
SEWPGPSGPPGPELQQPGDTAGLCPADARSGCA
LAVSQLPL*AA*GSGGPPRPHLPHSDTQPPADA
APSTGGPIHPAAPRSLSESAGHATS*DWRPGQPP
GAQPGLQPAAEPPSLSGGTSVLAAPCPAQQPPG
LCAS*LGPPSTPHPARPEGQPAPGPAP*AARRP
LCAPAGEPPG*GLARRPEFTSGSPHSRNAQTVPD
LRFGQLSCDPSRLLSDPGLWRPPAVPSGSHRHP
HHHPLSAAAAGARPRPPGSS*RPAQPCAGAAAP
WGGLPAGCGAVAALHPTAGPALP*SGGQDPE*QQ
LG*PGDLPGGRGTPAALGSLPGAPLLLVPCGFP
PCVQCLPGATGGDTAVLLGSSWGQSHLPPWGH*
GASSSLHAGGAHGWPRAAGPPGRTRGCSEPPAVP
VTERSPQLPPTGHRAAASALWHHRPQSGPLPPA
PVVLGPSCSHLG*HHSSGGPGAHPPVRTLPGHT
LLLVLALVHHQELCGRPGSEGLGAAAAAPCEPHR
SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG
PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC
GGQNLLCLLLAPEECEGGIRDHHSGPGGSGCAGP
GVLLPWRGACAGARGG*GCPAEEGRRRPVDGHS
AHQAAETSRVRGATAGGWPLLGTLESGRCRDRL
SDAEQPAECGWASGSGLASRGPAPGGVLPGGAAH
PARVPG*SG*ADPSHALLLG*APGWAARGCGAP
GAGPGAE*PAGRG*RGARSLGARPPQVPGQHPT
HGLGPQGPRSAWLLGSTAPRACPGLGPTDF*AGP
DIPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFT
SELDLFVG

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL
RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA
SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL
CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP
QSLSCLRSLVLKGGQRRDTLGACLRGALTNLPA
GLSGLAHLAHLDLSFNSLETLPACVLQMRGLGAL
LLSHNCLSELPEALGALPALTFLTVTHNRLQTL
PPALGALSTLQRLDLSQNLLDTLPPEIGGLGSL
LELNLASNRLQSLPASLAGLRSLRLLVLHSNLLA
SVPADLARLPLLTRLDLRDNQLRDLPPELLDAP
FVRLQGNPLGEASPDAPSSPVAALIPEMPRLFL
TSDLDSFPVTPQGCSVTLACGVRLQFPAGATATP
ITIRYRLLLPEPGLVPLGPHDALLSHVLELQPH
GVAFQQDVGLWLLFTPPQARRCREVVVRTRNDN
SWGDLETYLEEEAPQRLWAHCQVPHFSWFLVVSR
PVSNACLVPPEGTLLCSSGHPGVKVIFPPGATE
EPRRVSMQVVRMAGRELQALLGEPEAAVSPLLC
LSQSGPPSFLQPVTVQLPLPSGITGLSLDRSRLH
LLYWAPPAATWDDITAQVVLELTHLYARFQVTH
FSWYWLWYTTKNCVGGLARKAWERLRLHRVNLI
ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG
PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV
EGRICFVFYSHLKNVKEVYVTTTLDREAQAVRG
QVSFYRGAVPVRVPEEAEAARQRKGADALWMATL
PIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGF
LTQSNLLSVAGRLGLDWPAVALHLGVSYREVQR
IRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLL
VQALEQSDRQDVAEEVRAVLELGRRKYQDSIRR
MGLAPKDPALPGSSAPQPPEPAQA*APQTFRLA
QIFPSGWAEPPPSSLSSVWGRVPVSNKTALFLSP
QSLIYLLD

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC
VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML
QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT
CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL
RACPASAPWSSKEGNAGTHWVPVSGVP*PTCPL
V*VAWPIWPTWT*ASTAWRHCRPVSCRCEVWVR
SCCLTTASLSCLRLWGPSPPSPSSQ*HTTACRRC
PQHWGPYPPCSASISLRICWTRYLLRLEAWAAS
WSSTWPPTGCRASQPLWRDFGPCGSLSCTATSW
PLCQLTWPAFHSSPGST*GTTSSGTCPLSC*TPP
LCACRGTPWVRPRQTPRVHQWQPSFQKCPDCS*
PQIWTAFL*PLKAAQ*PWPVASACSSQREPPPP
PSPSAIGCCCRSQASSPWVLMTPCSAMCWSCSPM
GWPSSRMWGCGCSSPHRRPGAAVKWWSGPGMTT
AGVTWRPTWRKRHPSGSGLTARCPTSPGSLWFP
ALCPMPAWCHRRGHCCAPRVILGSKSSSPLGPLR
SLVESPCRWCAWLAESCRPSWENQRLQ*APCCA
CHRAVPPASSNRSPCSCLCPLASQASVWTAPAC
TCCTGPLLQPPGMTSQLRWSWSSPTCTHASRSHT
SPGTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS
LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG
APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW
RAESALSSTRT*RM*RRYT*PPLWTGRLRLCGA
RCPSTVARCLCGCPRRLRLPGRGRAQTPCGWPL
CPSSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF
*RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSA
SGTSSGMIWMSRSVTCSSPGLSARLGSQGLWGS
WCRPWSRVTGRTWLKRCAQSWSSAAASTRTASDA
WAWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWP
RYSPVDGQSPHLQVSPVCGDGSL*ATKLHCFFH
LRA*FICWM

Sequence LRDD.aAug05

GCCTGCGCCGCGGGCCTACCCGGAACGGCGCGTGGGCCCGGGCAGAAGCTGCTGGCGGTAGGCTCGCCTTGACTCCTTGGTGGTTCCCTGGCCGCGGCGA
CCGGTGGTCCCACGTCCAGCCGGGCTCAGTGAGACGGAGTCTTGCTCTGTCTCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTACAACCTCCGC
CTCCCGGGTTCACGCCATTCTTCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCGCATCACCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGA
CGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTCAGCCACC
GCGCCCGCTCAATATTTTATCTCTTAACGCCTCCTTCTCCCCTGCGCGATTGAAGGAGACAGTGCCAGGAGGCTCACTGCTTCTGGCCCAGAGTGATGGC
TCGAAAGGAAGGGTCTTTGCAGGCCCAGAGAGGAGGTCCTGGCAGGCTTCCCGGAGGAGGCCGTTTGGGCTGGTTTGGAAAGGACACTGCAATTGTTCCG
GCTTGTAGCAGGGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACG
GTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGC
TGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCAC
TCACGAGGACCCTCAGC
TGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGAGGGCAACGCCGGGAC
ACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGG
AGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCC
CGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAAT
CTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGG
GACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAG
GGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGT
TCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGG
CCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGG
TCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAG
GCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGG
CTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTC
GGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAG
GCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTC
TGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGT
GGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCT
CGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAA
ACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGC
CTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTG
TCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCTGCCCA
TCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGAC
GCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGG
CACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGC
AGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGCATGGG
CTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCCAGATA
TTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACCTCAGA
GCTTGATTTATTTGTTGGATG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA
TGGPTSSRAQ*DGVLLCLPGWSAVAPSRLTTTSA
SRVHAILLPQPPE*LGLQARITTPG*FLYF**R
RGFTMLARLVSNS*PRDPPASASQSAGITGVSH
RARSIFYLLTPPSPLRD*RRQCQEAHCFWPRVMA
RKEGSLQAQRGGPGRLPGGGRLGWFGKDTAIVP
ACSRASPGAAWTGLPACWDMSGLQGPSVGDGCN
GGGARAGGSCCRRRCFRGFGRRVQGAAFPGRQPA
ELGPVPRGLPAAAAPVCPAASAAAAGGILASEH
SRGPSA
AGGHPGPAASEPVLPPLPGPQRRATPG
HTGCLSPGCPDQPARWSEWPGPSGPPGPELQQPG
DTAGLCPADARSGCALAVSQLPL*AA*GSGGPP
RPHLPHSDTQPPADAAPSTGGPIHPAAPRSLSE
SAGHATS*DWRPGQPPGAQPGLQPAAEPPSLSGG
TSVLAAPCPAQQPPGLCAS*LGPPSTPHPARPE
GQPAPGPAP*AARRPLCAPAGEPPG*GLARRPE
FTSGSPHSRNAQTVPDLRFGQLSCDPSRLLSDPG
LWRPPAVPSGSHRHPHHHPLSAAAAGARPRPPG
SS*RPAQPCAGAAAPWGGLPAGCGAVAALHPTA
GPALP*SGGQDPE*QQLG*PGDLPGGRGTPAALG
SLPGAPLLLVPCGFPPCVQCLPGATGGDTAVLL
GSSWGQSHLPPWGH*GASSSLHAGGAHGWPRAA
GPPGRTRGCSEPPAVPVTERSPQLPPTGHRAAAS
ALWHHRPQSGPLPPAPVVLGPSCSHLG*HHSSG
GPGAHPPVRTLPGHTLLLVLALVHHQELCGRPG
SEGLGAAAAAPCEPHRSAAAPGP*AGPAAVPAPK
QGGRHPSAAAGAVPGPRAL*HGGDVRGRRVLCG
LRARHRRGC*PP*LCGGQNLLCLLLAPEECEGG
VLLPWRGACAGARGG*GCPAEEGRRRPVDGHSAH
QAAETSRVRGATAGGWPLLGTLESGRCRDRLSD
AEQPAECGWASGSGLASRGPAPGGVLPGGAAHP
ARVPG*SG*ADPSHALLLG*APGWAARGCGAPGA
GPGAE*PAGRG*RGARSLGARPPQVPGQHPTHG
LGPQGPRSAWLLGSTAPRACPGLGPTDF*AGPD
IPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFTSE
LDLFVG

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR
PVVPRPAGLSETESCSVSQAGVQWHHLGSLQPP
PPGFTPFFCLSLLSSWDYRRASPRPANFCIFSRD
GVSPCWPGWSQTPDLVIHPPRPPKVLGLQASAT
APAQYFIS*RLLLPCAIEGDSARRLTASGPE*W
LERKGLCRPREEVLAGFPEEAVWAGLERTLQLFR
LVAGLPRALPGQACLRAGTCLASKDRRWAMAAT
VEGPELEAAAAAGDASEDSDAGSRALPFLGGNR
LSLDLYPGGCQQLLHLCVQQPLQLLQVEFLRLST
HEDPQ
LLEATLAQLPQSLSCLRSLVLKGGQRRD
TLGACLRGALTNLPAGLSGLAHLAHLDLSFNSL
ETLPACVLQMRGLGALLLSHNCLSELPEALGALP
ALTFLTVTHNRLQTLPPALGALSTLQRLDLSQN
LLDTLPPEIGGLGSLLELNLASNRLQSLPASLA
GLRSLRLLVLHSNLLASVPADLARLPLLTRLDLR
DNQLRDLPPELLDAPFVRLQGNPLGEASPDAPS
SPVAALIPEMPRLFLTSDLDSFPVTPQGCSVTL
ACGVRLQFPAGATATPITIRYRLLLPEPGLVPLG
PHDALLSHVLELQPHGVAFQQDVGLWLLFTPPQ
ARRCREVVVRTRNDNSWGDLETYLEEEAPQRLW
AHCQVPHFSWFLVVSRPVSNACLVPPEGTLLCSS
GHPGVKVIFPPGATEEPRRVSMQVVRMAGRELQ
ALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQLP
LPSGITGLSLDRSRLHLLYWAPPAATWDDITAQV
VLELTHLYARFQVTHFSWYWLWYTTKNCVGGLA
RKAWERLRLHRVNLIALQRRRDPEQVLLQCLPR
NKVDATLRRLLERYRGPEPSDTVEMFEGEEFFAA
FERGIDVDADRPDCVEGRICFVFYSHLKNVKEV
SFYRGAVPVRVPEEAEAARQRKGADALWMATLP
IKLPRLRGSEGPRRGAGLSLAPLNLGDAETGFLT
QSNLLSVAGRLGLDWPAVALHLGVSYREVQRIR
HEFRDDLDEQIRHMLFSWAERQAGQPGAVGLLV
QALEQSDRQDVAEEVRAVLELGRRKYQDSIRRMG
LAPKDPALPGSSAPQPPEPAQA*APQTFRLAQI
FPSGWAEPPPSSLSSVWGRVPVSNKTALFLSPQ
SLIYLLD

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD
RWSHVQPGSVRRSLALSPRLECSGTISAHYNLR
LPGSRHSSASAS*VAGTTGAHHHARLIFVFLVE
TGFHHVGQAGLKLLTS*STRLGLPKCWDYRRQPP
RPLNILSLNASFSPARLKETVPGGSLLLAQSDG
SKGRVFAGPERRSWQASRRRPFGLVWKGHCNCS
GL*QGFPGRCLDRPACVLGHVWPPRTVGGRWLQR
WRGQSWRQLLPQEMLQRIRTQGPGRCLSWAATG
*AWTCTPGAASSCCTCVSSSLCSCCRWNSCV*A
LTRTLS
CWRPPWPSCLRACPASAPWSSKEGNAGT
HWVPVSGVP*PTCPLV*VAWPIWPTWT*ASTAW
RHCRPVSCRCEVWVRSCCLTTASLSCLRLWGPS
PPSPSSQ*HTTACRRCPQHWGPYPPCSASISLRI
CWTRYLLRLEAWAASWSSTWPPTGCRASQPLWR
DFGPCGSLSCTATSWPLCQLTWPAFHSSPGST*
GTTSSGTCPLSC*TPPLCACRGTPWVRPRQTPRV
HQWQPSFQKCPDCS*PQIWTAFL*PLKAAQ*PW
PVASACSSQREPPPPPSPSAIGCCCRSQASSPW
VLMTPCSAMCWSCSPMGWPSSRMWGCGCSSPHRR
PGAAVKWWSGPGMTTAGVTWRPTWRKRHPSGSG
LTARCPTSPGSLWFPALCPMPAWCHRRGHCCAP
RVILGSKSSSPLGPLRSLVESPCRWCAWLAESCR
PSWENQRLQ*APCCACHRAVPPASSNRSPCSCL
CPLASQASVWTAPACTCCTGPLLQPPGMTSQLR
WSWSSPTCTHASRSHTSPGTGSGTPPRTVWEAWL
GRPGSGCGCTV*TSSLCSGAGTLSRSCCSACPE
TRWTPPFGGCWSGTGAPSPLTRWRCSRAKSSLR
PSSAASTWMLTALTVWRAESALSSTRT*RM*RRC
PSTVARCLCGCPRRLRLPGRGRAQTPCGWPLCP
SSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF*
RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSASG
TSSGMIWMSRSVTCSSPGLSARLGSQGLWGSWC
RPWSRVTGRTWLKRCAQSWSSAAASTRTASDAW
AWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWPRY
SPVDGQSPHLQVSPVCGDGSL*ATKLHCFFHLR
A*FICWM

Sequence LRDD.lAug05

GCCTGCGCCGCGGGCCTACCCGGAACGGCGCGTGGGCCCGGGCAGAAGCTGCTGGCGGTAGGCTCGCCTTGACTCCTTGGTGGTTCCCTGGCCGCGGCGA
CCGGTGGTCCCACGTCCAGCCGGGCTCAGTGAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTC
GGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCC
TTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTG
GAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCA
AAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCG
GGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTC
CTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA
TGGPTSSRAQ*ASPGAAWTGLPACWDMSGLQGPS
VGDGCNGGGARAGGSCCRRRCFRGFGRRVQGAA
FPGRQPAELGPVPRGLPAAAAPVCPAASAAAAG
GILASEHSRGPSAAGGHPGPAASEPVLPPLPGPQ
RVDLGPRTLALRATSASPLPRRATPGHTGCLSP
GCPDQPARWSEWPGPSGPPGPELQQPGDTAGLC
PADARSGCALAVSQLPL*AA*

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR
PVVPRPAGLSELPRALPGQACLRAGTCLASKDR
RWAMAATVEGPELEAAAAAGDASEDSDAGSRALP
FLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQV
EFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVL
KGSIWDLGPWL*GPHPPPPFPGGQRRDTLGACLR
GALTNLPAGLSGLAHLAHLDLSFNSLETLPACV
LQMRGLGALLLSHNCLSELPE

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD
RWSHVQPGSVSFPGRCLDRPACVLGHVWPPRTV
GGRWLQRWRGQSWRQLLPQEMLQRIRTQGPGRC
LSWAATG*AWTCTPGAASSCCTCVSSSLCSCCRW
NSCV*ALTRTLSCWRPPWPSCLRACPASAPWSS
KGRSGTSDPGSEGHIRLPPSQEGNAGTHWVPVS
GVP*PTCPLV*VAWPIWPTWT*ASTAWRHCRPVS
CRCEVWVRSCCLTTASLSCL

Sequence LRDD.kAug05

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG
CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT
CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT
GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT
CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAG
GGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAG
CTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCT
CTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCG
ATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCC
AGCCTCTCTGGGTGAGTAGCCCCTGCGCCCCGACACACTGGCCCCACGGGAGGGTCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTCTGCACCCACAGG
CTTGGTCCACAGCTGCCTCTTGGTTGTCCCTCCACCTCCCTGGCCTTTGAGACTCCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCAGCTGTGGAGGAG
AGTCTGAAACAGCTGCTCTGGGAGGCGGCAGCAGGAGTGTCCCAGCGCCGTGGGCTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCCCGCAGCGGGACT
TCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGAC
AACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCAC
CAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCC
CAGCGGGAGCCA
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP
ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF
RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP
VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA
SEPVLPPLPGPQRVDLGPRTLALRATSASPLPRR
ATPGHTGCLSPGCPDQPARWSEWPGPSGPPGPE
LQQPGDTAGLCPADARSGCALAVSQLPL*AA*G
SGGPPRPHLPHSDTQPPADAAPSTGGPIHPAAPR
SLSESAGHATS*DWRPGQPPGAQPGLQPAAEPP
SLSG*VAPAPRHTGPTGGSLKPACLLQGPLHPQ
AWSTAASWLSLHLPGL*DSLSGFVRVL*AQLWRR
V*NSCSGRRQQECPSAVGWAGAKPKPATARSGT
SVLAAPCPAQQPPGLCAS*LGPPSTPHPARPEG
QPAPGPAP*AARRPLCAPAGEPPG*GLARRPEFT
SGSPHSRNAQTVPDLRFGQAAQ*PWPVASACSS
QREP

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL
RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA
SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL
CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP
QSLSCLRSLVLKGSIWDLGPWL*GPHPPPPFPG
GQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLS
FNSLETLPACVLQMRGLGALLLSHNCLSELPEA
LGALPALTFLTVTHNRLQTLPPALGALSTLQRL
DLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLP
ASLGE*PLRPDTLAPREGP*SLPVFCRGLCTHR
LGPQLPLGCPSTSLAFETPSVALSEFSEPSCGG
ESETAALGGGSRSVPAPWAGLVPSLSQQLPAAGL
RSLRLLVLHSNLLASVPADLARLPLLTRLDLRD
NQLRDLPPELLDAPFVRLQGNPLGEASPDAPSS
PVAALIPEMPRLFLTSDLDRLLSDPGLWRPPAVP
SGS

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC
VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML
QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT
CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL
RACPASAPWSSKGRSGTSDPGSEGHIRLPPSQE
GNAGTHWVPVSGVP*PTCPLV*VAWPIWPTWT*
ASTAWRHCRPVSCRCEVWVRSCCLTTASLSCLRL
WGPSPPSPSSQ*HTTACRRCPQHWGPYPPCSAS
ISLRICWTRYLLRLEAWAASWSSTWPPTGCRAS
QPLWVSSPCAPTHWPHGRVPEACLSSAGASAPTG
LVHSCLLVVPPPPWPLRLPQWLCQSSLSPAVEE
SLKQLLWEAAAGVSQRRGLGWCQA*ASNCPQRD
FGPCGSLSCTATSWPLCQLTWPAFHSSPGST*GT
TSSGTCPLSC*TPPLCACRGTPWVRPRQTPRVH
QWQPSFQKCPDCS*PQIWTGCSVTLACGVRLQF
PAGA

Sequence LRDD.eAug05

CCCCGACGCCCGGGGGATCCCGAGCCTGCCTCGGGGCTGGGGCCCGCCCCCCACGCCGTCTGAGCGGTGCGCGGTGCACCACCCTCCCCAGTGGACCGAA
CTGGAAAGGCCGGGAGAGGTTTCCTCCGGGGCTCACGAATTGGGGTCCCGGCCCCAGGACCCCGAAGCTGCCCAATGGCTCACGCCCCAGGCCCTCACCC
TTACACGGCCTTCCCTGACTTTCGGGGCGCAGTCACCCCCGCGGTAGGGGAGGGTCACGGCCTTCCTGGTAAAACGGATGTAAATAGAAGGGGGACTCAT
CTCCAGGACTGGGGAACAGCCCCTATGACCTCGCAGCTCCACGGCTCCATTGGCCAGATTTCCAGGACCAGGACTGAAGCCTCACTACCCCGGCTTTGCT
GCTCATAGCGAGGTCTCCCTGCCCGGTGCTCCTGCGAGCCATGGCGGTCCCATTCCCCAACCCTCTTTCCAGTGGTGACCCAGGCCTCCGGGGTTCAGGG
TGACCACAAGGTCTCCCCCACTCTGCAGCCCAGGCCCAGATGGCCTGGCTCTAGAGCTCCCCACTCCATCCAGAGTCCCTGTTTCCCCAAAGAGAAGGGC
CCACCCCGGCTCCCGCTCACTCCTCCTCCTGCCTCTGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAG
GACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGG
CGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCT
GCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTG
GTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCT
GTCTCC
GGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGC
CTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTC
CTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGC
TACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCG
CCCCGACACACTGGCCCCACGGGAGGGTCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTCTGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGT
CCCTCCACCTCCCTGGCCTTTGAGACTCCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCAGCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCG
GCAGCAGGAGTGTCCCAGCGCCGTGGGCTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCCCGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCA
CAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAG
CTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGC
CCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGG
AGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTG
GAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGA
CCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTT
CCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCC
CCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGA
GCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGA
CCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGC
TTCCAGGTCACACACTTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACC
GTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCT
GGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGAC
CGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTC
AGGCTGTGCGGGGCCAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCT
GTGGATGGCCACTCTGCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGAT
GCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACC
GGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGG
GGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAG
GACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGA
CTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGC
ACTGTTTCTTTCACCTCAGAGCTTGATTTATTTGTTGGATG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

PRRPGDPEPASGLGPAPHAV*AVRGAPPSPVDR
TGKAGRGFLRGSRIGVPAPGPRSCPMAHAPGPHP
YTAFPDFRGAVTPAVGEGHGLPGKTDVNRRGTH
LQDWGTAPMTSQLHGSIGQISRTRTEASLPRLC
CS*RGLPARCSCEPWRSHSPTLFPVVTQASGVQG
DHKVSPTLQPRPRWPGSRAPHSIQSPCFPKEKG
PPRLPLTPPPASAASPGAAWTGLPACWDMSGLQ
GPSVGDGCNGGGARAGGSCCRRRCFRGFGRRVQG
AAFPGRQPAELGPVPRGLPAAAAPVCPAASAAA
AGGILASEHSRGPSAAGGHPGPAASEPVLPPLP
GPQRVDLGPRTLALRATSASPLPRRATPGHTGCL
SP
GCPDQPARWSEWPGPSGPPGPELQQPGDTAG
LCPADARSGCALAVSQLPL*AA*GSGGPPRPHL
PHSDTQPPADAAPSTGGPIHPAAPRSLSESAGHA
TS*DWRPGQPPGAQPGLQPAAEPPSLSG*VAPA
PRHTGPTGGSLKPACLLQGPLHPQAWSTAASWL
SLHLPGL*DSLSGFVRVL*AQLWRRV*NSCSGRR
QQECPSAVGWAGAKPKPATARSGTSVLAAPCPA
QQPPGLCAS*LGPPSTPHPARPEGQPAPGPAP*
AARRPLCAPAGEPPG*GLARRPEFTSGSPHSRNA
QTVPDLRFGQLSCDPSRLLSDPGLWRPPAVPSG
SHRHPHHHPLSAAAAGARPRPPGSS*RPAQPCA
GAAAPWGGLPAGCGAVAALHPTAGPALP*SGGQD
PE*QQLG*PGDLPGGRGTPAALGSLPGAPLLLV
PCGFPPCVQCLPGATGGDTAVLLGSSWGQSHLP
PWGH*GASSSLHAGGAHGWPRAAGPPGRTRGCSE
PPAVPVTERSPQLPPTGHRAAASALWHHRPQSG
PLPPAPVVLGPSCSHLG*HHSSGGPGAHPPVRT
LPGHTLLLVLALVHHQELCGRPGSEGLGAAAAAP
CEPHRSAAAPGP*AGPAAVPAPKQGGRHPSAAA
GAVPGPRAL*HGGDVRGRRVLCGLRARHRRGC*
PP*LCGGQNLLCLLLAPEECEGGIRDHHSGPGGS
GCAGPGVLLPWRGACAGARGG*GCPAEEGRRRP
VDGHSAHQAAETSRVRGATAGGWPLLGTLESGR
CRDRLSDAEQPAECGWASGSGLASRGPAPGGVLP
GGAAHPARVPG*SG*ADPSHALLLG*APGWAAR
GCGAPGAGPGAE*PAGRG*RGARSLGARPPQVP
GQHPTHGLGPQGPRSAWLLGSTAPRACPGLGPTD
F*AGPDIPQWMGRAPTFKSLQCVGTGPCEQQNC
TVSFTSELDLFVG

Frame 1

PDARGIPSLPRGWGPPPTPSERCAVHHPPQWTE
LERPGEVSSGAHELGSRPQDPEAAQWLTPQALT
LTRPSLTFGAQSPPR*GRVTAFLVKRM*IEGGLI
SRTGEQPL*PRSSTAPLARFPGPGLKPHYPGFA
AHSEVSLPGAPASHGGPIPQPSFQW*PRPPGFR
VTTRSPPLCSPGPDGLALELPTPSRVPVSPKRRA
HPGSRSLLLLPLQLPRALPGQACLRAGTCLASK
DRRWAMAATVEGPELEAAAAAGDASEDSDAGSR
ALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLL
QVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSL
VLKGSIWDLGPWL*GPHPPPPFPGGQRRDTLGA
CL
RGALTNLPAGLSGLAHLAHLDLSFNSLETLPA
CVLQMRGLGALLLSHNCLSELPEALGALPALTF
LTVTHNRLQTLPPALGALSTLQRLDLSQNLLDT
LPPEIGGLGSLLELNLASNRLQSLPASLGE*PLR
PDTLAPREGP*SLPVFCRGLCTHRLGPQLPLGC
PSTSLAFETPSVALSEFSEPSCGGESETAALGG
GSRSVPAPWAGLVPSLSQQLPAAGLRSLRLLVLH
SNLLASVPADLARLPLLTRLDLRDNQLRDLPPE
LLDAPFVRLQGNPLGEASPDAPSSPVAALIPEM
PRLFLTSDLDSFPVTPQGCSVTLACGVRLQFPAG
ATATPITIRYRLLLPEPGLVPLGPHDALLSHVL
ELQPHGVAFQQDVGLWLLFTPPQARRCREVVVR
TRNDNSWGDLETYLEEEAPQRLWAHCQVPHFSWF
LVVSRPVSNACLVPPEGTLLCSSGHPGVKVIFP
PGATEEPRRVSMQVVRMAGRELQALLGEPEAAV
SPLLCLSQSGPPSFLQPVTVQLPLPSGITGLSLD
RSRLHLLYWAPPAATWDDITAQVVLELTHLYAR
FQVTHFSWYWLWYTTKNCVGGLARKAWERLRLH
RVNLIALQRRRDPEQVLLQCLPRNKVDATLRRLL
ERYRGPEPSDTVEMFEGEEFFAAFERGIDVDAD
RPDCVEGRICFVFYSHLKNVKEVYVTTTLDREA
QAVRGQVSFYRGAVPVRVPEEAEAARQRKGADAL
WMATLPIKLPRLRGSEGPRRGAGLSLAPLNLGD
AETGFLTQSNLLSVAGRLGLDWPAVALHLGVSY
REVQRIRHEFRDDLDEQIRHMLFSWAERQAGQPG
AVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQ
DSIRRMGLAPKDPALPGSSAPQPPEPAQA*APQ
TFRLAQIFPSGWAEPPPSSLSSVWGRVPVSNKTA
LFLSPQSLIYLLD

Frame 2

PTPGGSRACLGAGARPPRRLSGARCTTLPSGPN
WKGRERFPPGLTNWGPGPRTPKLPNGSRPRPSP
LHGLP*LSGRSHPRGRGGSRPSW*NGCK*KGDS
SPGLGNSPYDLAAPRLHWPDFQDQD*SLTTPALL
LIARSPCPVLLRAMAVPFPNPLSSGDPGLRGSG
*PQGLPHSAAQAQMAWL*SSPLHPESLFPQREG
PTPAPAHSSSCLCSFPGRCLDRPACVLGHVWPPR
TVGGRWLQRWRGQSWRQLLPQEMLQRIRTQGPG
RCLSWAATG*AWTCTPGAASSCCTCVSSSLCSC
CRWNSCV*ALTRTLSCWRPPWPSCLRACPASAPW
SSKGRSGTSDPGSEGHIRLPPSQEGNAGTHWVP
VS
GVP*PTCPLV*VAWPIWPTWT*ASTAWRHCR
PVSCRCEVWVRSCCLTTASLSCLRLWGPSPPSPS
SQ*HTTACRRCPQHWGPYPPCSASISLRICWTR
YLLRLEAWAASWSSTWPPTGCRASQPLWVSSPC
APTHWPHGRVPEACLSSAGASAPTGLVHSCLLVV
PPPPWPLRLPQWLCQSSLSPAVEESLKQLLWEA
AAGVSQRRGLGWCQA*ASNCPQRDFGPCGSLSC
TATSWPLCQLTWPAFHSSPGST*GTTSSGTCPLS
C*TPPLCACRGTPWVRPRQTPRVHQWQPSFQKC
PDCS*PQIWTAFL*PLKAAQ*PWPVASACSSQR
EPPPPPSPSAIGCCCRSQASSPWVLMTPCSAMCW
SCSPMGWPSSRMWGCGCSSPHRRPGAAVKWWSG
PGMTTAGVTWRPTWRKRHPSGSGLTARCPTSPG
SLWFPALCPMPAWCHRRGHCCAPRVILGSKSSSP
LGPLRSLVESPCRWCAWLAESCRPSWENQRLQ*
APCCACHRAVPPASSNRSPCSCLCPLASQASVW
TAPACTCCTGPLLQPPGMTSQLRWSWSSPTCTHA
SRSHTSPGTGSGTPPRTVWEAWLGRPGSGCGCT
V*TSSLCSGAGTLSRSCCSACPETRWTPPFGGC
WSGTGAPSPLTRWRCSRAKSSLRPSSAASTWMLT
ALTVWRAESALSSTRT*RM*RRYT*PPLWTGRL
RLCGARCPSTVARCLCGCPRRLRLPGRGRAQTP
CGWPLCPSSCRDFEGPRGHGGGLASPWHP*IWEM
PRPAF*RRATC*VWLGVWVWTGQPWPCTWGCPT
GRCSASGTSSGMIWMSRSVTCSSPGLSARLGSQ
GLWGSWCRPWSRVTGRTWLKRCAQSWSSAAASTR
TASDAWAWPPRTPLCLAPRLHSPQSLPRPRPHR
LLGWPRYSPVDGQSPHLQVSPVCGDGSL*ATKL
HCFFHLRA*FICWM

Sequence LRDD.dAug05

CCCCGACGCCCGGGGGATCCCGAGCCTGCCTCGGGGCTGGGGCCCGCCCCCCACGCCGTCTGAGCGGTGCGCGGTGCACCACCCTCCCCAGTGGACCGAA
CTGGAAAGGCCGGGAGAGGTTTCCTCCGGGGCTCACGAATTGGGGTCCCGGCCCCAGGACCCCGAAGCTGCCCAATGGCTCACGCCCCAGGCCCTCACCC
TTACACGGCCTTCCCTGACTTTCGGGGCGCAGTCACCCCCGCGGTAGGGGAGGGTCACGGCCTTCCTGGTAAAACGGATGTAAATAGAAGGGGGACTCAT
CTCCAGGACTGGGGAACAGCCCCTATGACCTCGCAGCTCCACGGCTCCATTGGCCAGATTTCCAGGACCAGGACTGAAGCCTCACTACCCCGGCTTTGCT
GCTCATAGCGAGGTCTCCCTGCCCGGTGCTCCTGCGAGCCATGGCGGTCCCATTCCCCAACCCTCTTTCCAGTGGTGACCCAGGCCTCCGGGGTTCAGGG
TGACCACAAGGTCTCCCCCACTCTGCAGCCCAGGCCCAGATGGCCTGGCTCTAGAGCTCCCCACTCCATCCAGAGTCCCTGTTTCCCCAAAGAGAAGGGC
CCACCCCGGCTCCCGCTCACTCCTCCTCCTGCCTCTGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAG
GACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGG
CGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCT
GCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTG
GTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCT
GTCTCC
GGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGC
CTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTC
CTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGC
TACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGGGACTTCGGTCCTT
GCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTC
CGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAG
CCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCG
CCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCC
CTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCC
GTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGT
GCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGG
GTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAG
AACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCAT
CACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTC
ACCCACCTGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG
CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG
CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG
GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTCAGGCTGTGCGGGGCC
AGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCT
GCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTT
CTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCA
TCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCT
GGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGC
ATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCC
AGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACC
TCAGAGCTTGATTTATTTGTTGGATG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

PRRPGDPEPASGLGPAPHAV*AVRGAPPSPVDR
TGKAGRGFLRGSRIGVPAPGPRSCPMAHAPGPHP
YTAFPDFRGAVTPAVGEGHGLPGKTDVNRRGTH
LQDWGTAPMTSQLHGSIGQISRTRTEASLPRLC
CS*RGLPARCSCEPWRSHSPTLFPVVTQASGVQG
DHKVSPTLQPRPRWPGSRAPHSIQSPCFPKEKG
PPRLPLTPPPASAASPGAAWTGLPACWDMSGLQ
GPSVGDGCNGGGARAGGSCCRRRCFRGFGRRVQG
AAFPGRQPAELGPVPRGLPAAAAPVCPAASAAA
AGGILASEHSRGPSAAGGHPGPAASEPVLPPLP
GPQRVDLGPRTLALRATSASPLPRRATPGHTGCL
SP
GCPDQPARWSEWPGPSGPPGPELQQPGDTAG
LCPADARSGCALAVSQLPL*AA*GSGGPPRPHL
PHSDTQPPADAAPSTGGPIHPAAPRSLSESAGHA
TS*DWRPGQPPGAQPGLQPAAEPPSLSGGTSVL
AAPCPAQQPPGLCAS*LGPPSTPHPARPEGQPA
PGPAP*AARRPLCAPAGEPPG*GLARRPEFTSGS
PHSRNAQTVPDLRFGQLSCDPSRLLSDPGLWRP
PAVPSGSHRHPHHHPLSAAAAGARPRPPGSS*R
PAQPCAGAAAPWGGLPAGCGAVAALHPTAGPALP
*SGGQDPE*QQLG*PGDLPGGRGTPAALGSLPG
APLLLVPCGFPPCVQCLPGATGGDTAVLLGSSW
GQSHLPPWGH*GASSSLHAGGAHGWPRAAGPPGR
TRGCSEPPAVPVTERSPQLPPTGHRAAASALWH
HRPQSGPLPPAPVVLGPSCSHLG*HHSSGGPGA
HPPVLALVHHQELCGRPGSEGLGAAAAAPCEPHR
SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG
PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC
GGQNLLCLLLAPEECEGGIRDHHSGPGGSGCAGP
GVLLPWRGACAGARGG*GCPAEEGRRRPVDGHS
AHQAAETSRVRGATAGGWPLLGTLESGRCRDRL
SDAEQPAECGWASGSGLASRGPAPGGVLPGGAAH
PARVPG*SG*ADPSHALLLG*APGWAARGCGAP
GAGPGAE*PAGRG*RGARSLGARPPQVPGQHPT
HGLGPQGPRSAWLLGSTAPRACPGLGPTDF*AGP
DIPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFT
SELDLFVG

Frame 1

PDARGIPSLPRGWGPPPTPSERCAVHHPPQWTE
LERPGEVSSGAHELGSRPQDPEAAQWLTPQALT
LTRPSLTFGAQSPPR*GRVTAFLVKRM*IEGGLI
SRTGEQPL*PRSSTAPLARFPGPGLKPHYPGFA
AHSEVSLPGAPASHGGPIPQPSFQW*PRPPGFR
VTTRSPPLCSPGPDGLALELPTPSRVPVSPKRRA
HPGSRSLLLLPLQLPRALPGQACLRAGTCLASK
DRRWAMAATVEGPELEAAAAAGDASEDSDAGSR
ALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLL
QVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSL
VLKGSIWDLGPWL*GPHPPPPFPGGQRRDTLGA
CL
RGALTNLPAGLSGLAHLAHLDLSFNSLETLPA
CVLQMRGLGALLLSHNCLSELPEALGALPALTF
LTVTHNRLQTLPPALGALSTLQRLDLSQNLLDT
LPPEIGGLGSLLELNLASNRLQSLPASLAGLRSL
RLLVLHSNLLASVPADLARLPLLTRLDLRDNQL
RDLPPELLDAPFVRLQGNPLGEASPDAPSSPVA
ALIPEMPRLFLTSDLDSFPVTPQGCSVTLACGVR
LQFPAGATATPITIRYRLLLPEPGLVPLGPHDA
LLSHVLELQPHGVAFQQDVGLWLLFTPPQARRC
REVVVRTRNDNSWGDLETYLEEEAPQRLWAHCQV
PHFSWFLVVSRPVSNACLVPPEGTLLCSSGHPG
VKVIFPPGATEEPRRVSMQVVRMAGRELQALLG
EPEAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGI
TGLSLDRSRLHLLYWAPPAATWDDITAQVVLEL
THLYWLWYTTKNCVGGLARKAWERLRLHRVNLI
ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG
PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV
EGRICFVFYSHLKNVKEVYVTTTLDREAQAVRG
QVSFYRGAVPVRVPEEAEAARQRKGADALWMATL
PIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGF
LTQSNLLSVAGRLGLDWPAVALHLGVSYREVQR
IRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLL
VQALEQSDRQDVAEEVRAVLELGRRKYQDSIRR
MGLAPKDPALPGSSAPQPPEPAQA*APQTFRLA
QIFPSGWAEPPPSSLSSVWGRVPVSNKTALFLSP
QSLIYLLD

Frame 2

PTPGGSRACLGAGARPPRRLSGARCTTLPSGPN
WKGRERFPPGLTNWGPGPRTPKLPNGSRPRPSP
LHGLP*LSGRSHPRGRGGSRPSW*NGCK*KGDS
SPGLGNSPYDLAAPRLHWPDFQDQD*SLTTPALL
LIARSPCPVLLRAMAVPFPNPLSSGDPGLRGSG
*PQGLPHSAAQAQMAWL*SSPLHPESLFPQREG
PTPAPAHSSSCLCSFPGRCLDRPACVLGHVWPPR
TVGGRWLQRWRGQSWRQLLPQEMLQRIRTQGPG
RCLSWAATG*AWTCTPGAASSCCTCVSSSLCSC
CRWNSCV*ALTRTLSCWRPPWPSCLRACPASAPW
SSKGRSGTSDPGSEGHIRLPPSQEGNAGTHWVP
VS
GVP*PTCPLV*VAWPIWPTWT*ASTAWRHCR
PVSCRCEVWVRSCCLTTASLSCLRLWGPSPPSPS
SQ*HTTACRRCPQHWGPYPPCSASISLRICWTR
YLLRLEAWAASWSSTWPPTGCRASQPLWRDFGP
CGSLSCTATSWPLCQLTWPAFHSSPGST*GTTSS
GTCPLSC*TPPLCACRGTPWVRPRQTPRVHQWQ
PSFQKCPDCS*PQIWTAFL*PLKAAQ*PWPVAS
ACSSQREPPPPPSPSAIGCCCRSQASSPWVLMTP
CSAMCWSCSPMGWPSSRMWGCGCSSPHRRPGAA
VKWWSGPGMTTAGVTWRPTWRKRHPSGSGLTAR
CPTSPGSLWFPALCPMPAWCHRRGHCCAPRVILG
SKSSSPLGPLRSLVESPCRWCAWLAESCRPSWE
NQRLQ*APCCACHRAVPPASSNRSPCSCLCPLA
SQASVWTAPACTCCTGPLLQPPGMTSQLRWSWSS
PTCTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS
LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG
APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW
RAESALSSTRT*RM*RRYT*PPLWTGRLRLCGA
RCPSTVARCLCGCPRRLRLPGRGRAQTPCGWPL
CPSSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF
*RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSA
SGTSSGMIWMSRSVTCSSPGLSARLGSQGLWGS
WCRPWSRVTGRTWLKRCAQSWSSAAASTRTASDA
WAWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWP
RYSPVDGQSPHLQVSPVCGDGSL*ATKLHCFFH
LRA*FICWM

Sequence LRDD.cAug05

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG
CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT
CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT
GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT
CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAG
GGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAG
CTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCT
CTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCG
ATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCC
AGCCTCTCTGGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACC
CGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGC
CAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTG
CTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGC
CTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCT
TCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACC
CCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACA
CTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTG
GCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCAC
CGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGAC
ATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGC
TGCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCT
TCGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGAC
GTGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGG
ACCGGGAGGCTCAGGCTGTGCGGGGCCAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGG
CGCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTG
AATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGG
GGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGC
TGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGC
CGCAAGTACCAGGACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCT
AGGCCCCACAGACTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAG
CAACAAAACTGCACTGTTTCTTTCACCTCAGAGCTTGATTTATTTGTTGGATG
  • LRDD.u.f.7 (forward primer)
  • LRDD.u.r.3 (reverse primer)
  • Alternative acceptor

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP
ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF
RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP
VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA
SEPVLPPLPGPQRVDLGPRTLALRATSASPLPRR
ATPGHTGCLSPGCPDQPARWSEWPGPSGPPGPE
LQQPGDTAGLCPADARSGCALAVSQLPL*AA*G
SGGPPRPHLPHSDTQPPADAAPSTGGPIHPAAPR
SLSESAGHATS*DWRPGQPPGAQPGLQPAAEPP
SLSGGTSVLAAPCPAQQPPGLCAS*LGPPSTPH
PARPEGQPAPGPAP*AARRPLCAPAGEPPG*GLA
RRPEFTSGSPHSRNAQTVPDLRFGQLSCDPSRL
LSDPGLWRPPAVPSGSHRHPHHHPLSAAAAGAR
PRPPGSS*RPAQPCAGAAAPWGGLPAGCGAVAAL
HPTAGPALP*SGGQDPE*QQLG*PGDLPGGRGT
PAALGSLPGAPLLLVPCGFPPCVQCLPGATGGD
TAVLLGSSWGQSHLPPWGH*GASSSLHAGGAHGW
PRAAGPPGRTRGCSEPPAVPVTERSPQLPPTGH
RAAASALWHHRPQSGPLPPAPVVLGPSCSHLG*
HHSSGGPGAHPPVLALVHHQELCGRPGSEGLGAA
AAAPCEPHRSAAAPGP*AGPAAVPAPKQGGRHP
SAAAGAVPGPRAL*HGGDVRGRRVLCGLRARHR
RGC*PP*LCGGQNLLCLLLAPEECEGGIRDHHSG
PGGSGCAGPGVLLPWRGACAGARGG*GCPAEEG
RRRPVDGHSAHQAAETSRVRGATAGGWPLLGTL
ESGRCRDRLSDAEQPAECGWASGSGLASRGPAPG
GVLPGGAAHPARVPG*SG*ADPSHALLLG*APG
WAARGCGAPGAGPGAE*PAGRG*RGARSLGARP
PQVPGQHPTHGLGPQGPRSAWLLGSTAPRACPGL
GPTDF*AGPDIPQWMGRAPTFKSLQCVGTGPCE
QQNCTVSFTSELDLFVG

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL
RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA
SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL
CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP
QSLSCLRSLVLKGSIWDLGPWL*GPHPPPPFPG
GQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLS
FNSLETLPACVLQMRGLGALLLSHNCLSELPEA
LGALPALTFLTVTHNRLQTLPPALGALSTLQRL
DLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLP
ASLAGLRSLRLLVLHSNLLASVPADLARLPLLT
RLDLRDNQLRDLPPELLDAPFVRLQGNPLGEAS
PDAPSSPVAALIPEMPRLFLTSDLDSFPVTPQGC
SVTLACGVRLQFPAGATATPITIRYRLLLPEPG
LVPLGPHDALLSHVLELQPHGVAFQQDVGLWLL
FTPPQARRCREVVVRTRNDNSWGDLETYLEEEAP
QRLWAHCQVPHFSWFLVVSRPVSNACLVPPEGT
LLCSSGHPGVKVIFPPGATEEPRRVSMQVVRMA
GRELQALLGEPEAAVSPLLCLSQSGPPSFLQPVT
VQLPLPSGITGLSLDRSRLHLLYWAPPAATWDD
ITAQVVLELTHLYWLWYTTKNCVGGLARKAWER
LRLHRVNLIALQRRRDPEQVLLQCLPRNKVDATL
RRLLERYRGPEPSDTVEMFEGEEFFAAFERGID
VDADRPDCVEGRICFVFYSHLKNVKEVYVTTTL
DREAQAVRGQVSFYRGAVPVRVPEEAEAARQRKG
ADALWMATLPIKLPRLRGSEGPRRGAGLSLAPL
NLGDAETGFLTQSNLLSVAGRLGLDWPAVALHL
GVSYREVQRIRHEFRDDLDEQIRHMLFSWAERQA
GQPGAVGLLVQALEQSDRQDVAEEVRAVLELGR
RKYQDSIRRMGLAPKDPALPGSSAPQPPEPAQA
*APQTFRLAQIFPSGWAEPPPSSLSSVWGRVPVS
NKTALFLSPQSLIYLLD

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC
VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML
QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT
CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL
RACPASAPWSSKGRSGTSDPGSEGHIRLPPSQE
GNAGTHWVPVSGVP*PTCPLV*VAWPIWPTWT*
ASTAWRHCRPVSCRCEVWVRSCCLTTASLSCLRL
WGPSPPSPSSQ*HTTACRRCPQHWGPYPPCSAS
ISLRICWTRYLLRLEAWAASWSSTWPPTGCRAS
QPLWRDFGPCGSLSCTATSWPLCQLTWPAFHSSP
GST*GTTSSGTCPLSC*TPPLCACRGTPWVRPR
QTPRVHQWQPSFQKCPDCS*PQIWTAFL*PLKA
AQ*PWPVASACSSQREPPPPPSPSAIGCCCRSQA
SSPWVLMTPCSAMCWSCSPMGWPSSRMWGCGCS
SPHRRPGAAVKWWSGPGMTTAGVTWRPTWRKRH
PSGSGLTARCPTSPGSLWFPALCPMPAWCHRRGH
CCAPRVILGSKSSSPLGPLRSLVESPCRWCAWL
AESCRPSWENQRLQ*APCCACHRAVPPASSNRS
PCSCLCPLASQASVWTAPACTCCTGPLLQPPGMT
SQLRWSWSSPTCTGSGTPPRTVWEAWLGRPGSG
CGCTV*TSSLCSGAGTLSRSCCSACPETRWTPP
FGGCWSGTGAPSPLTRWRCSRAKSSLRPSSAAST
WMLTALTVWRAESALSSTRT*RM*RRYT*PPLW
TGRLRLCGARCPSTVARCLCGCPRRLRLPGRGR
AQTPCGWPLCPSSCRDFEGPRGHGGGLASPWHP*
IWEMPRPAF*RRATC*VWLGVWVWTGQPWPCTW
GCPTGRCSASGTSSGMIWMSRSVTCSSPGLSAR
LGSQGLWGSWCRPWSRVTGRTWLKRCAQSWSSAA
ASTRTASDAWAWPPRTPLCLAPRLHSPQSLPRP
RPHRLLGWPRYSPVDGQSPHLQVSPVCGDGSL*
ATKLHCFFHLRA*FICWM

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