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Sequences

Sequence LRDD.lAug05

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA TGGPTSSRAQ*ASPGAAWTGLPACWDMSGLQGPS VGDGCNGGGARAGGSCCRRRCFRGFGRRVQGAA FPGRQPAELGPVPRGLPAAAAPVCPAASAAAAG GILASEHSRGPSAAGGHPGPAASEPVLPPLPGPQ RVDLGPRTLALRATSASPLPRRATPGHTGCLSP GCPDQPARWSEWPGPSGPPGPELQQPGDTAGLC PADARSGCALAVSQLPL*AA*

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR PVVPRPAGLSELPRALPGQACLRAGTCLASKDR RWAMAATVEGPELEAAAAAGDASEDSDAGSRALP FLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQV EFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVL KGSIWDLGPWL*GPHPPPPFPGGQRRDTLGACLR GALTNLPAGLSGLAHLAHLDLSFNSLETLPACV LQMRGLGALLLSHNCLSELPE

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD RWSHVQPGSVSFPGRCLDRPACVLGHVWPPRTV GGRWLQRWRGQSWRQLLPQEMLQRIRTQGPGRC LSWAATG*AWTCTPGAASSCCTCVSSSLCSCCRW NSCV*ALTRTLSCWRPPWPSCLRACPASAPWSS KGRSGTSDPGSEGHIRLPPSQEGNAGTHWVPVS GVP*PTCPLV*VAWPIWPTWT*ASTAWRHCRPVS CRCEVWVRSCCLTTASLSCL

Sequence LRDD.kAug05

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

Frame 1

Frame 2

Sequence LRDD.eAug05

CCCCGACGCCCGGGGGATCCCGAGCCTGCCTCGGGGCTGGGGCCCGCCCCCCACGCCGTCTGAGCGGTGCGCGGTGCACCACCCTCCCCAGTGGACCGAA CTGGAAAGGCCGGGAGAGGTTTCCTCCGGGGCTCACGAATTGGGGTCCCGGCCCCAGGACCCCGAAGCTGCCCAATGGCTCACGCCCCAGGCCCTCACCC TTACACGGCCTTCCCTGACTTTCGGGGCGCAGTCACCCCCGCGGTAGGGGAGGGTCACGGCCTTCCTGGTAAAACGGATGTAAATAGAAGGGGGACTCAT CTCCAGGACTGGGGAACAGCCCCTATGACCTCGCAGCTCCACGGCTCCATTGGCCAGATTTCCAGGACCAGGACTGAAGCCTCACTACCCCGGCTTTGCT GCTCATAGCGAGGTCTCCCTGCCCGGTGCTCCTGCGAGCCATGGCGGTCCCATTCCCCAACCCTCTTTCCAGTGGTGACCCAGGCCTCCGGGGTTCAGGG TGACCACAAGGTCTCCCCCACTCTGCAGCCCAGGCCCAGATGGCCTGGCTCTAGAGCTCCCCACTCCATCCAGAGTCCCTGTTTCCCCAAAGAGAAGGGC CCACCCCGGCTCCCGCTCACTCCTCCTCCTGCCTCTGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAG GACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGG CGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCT GCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTG GTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCT GTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGC CTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTC CTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGC TACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCG CCCCGACACACTGGCCCCACGGGAGGGTCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTCTGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGT CCCTCCACCTCCCTGGCCTTTGAGACTCCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCAGCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCG GCAGCAGGAGTGTCCCAGCGCCGTGGGCTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCCCGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCA CAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAG CTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGC CCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGG AGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTG GAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGA CCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTT CCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCC CCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGA GCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGA CCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGC TTCCAGGTCACACACTTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACC GTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCT GGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGAC CGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTC AGGCTGTGCGGGGCCAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCT GTGGATGGCCACTCTGCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGAT GCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACC GGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGG GGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAG GACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGA CTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGC ACTGTTTCTTTCACCTCAGAGCTTGATTTATTTGTTGGATG

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

PRRPGDPEPASGLGPAPHAV*AVRGAPPSPVDR TGKAGRGFLRGSRIGVPAPGPRSCPMAHAPGPHP YTAFPDFRGAVTPAVGEGHGLPGKTDVNRRGTH LQDWGTAPMTSQLHGSIGQISRTRTEASLPRLC CS*RGLPARCSCEPWRSHSPTLFPVVTQASGVQG DHKVSPTLQPRPRWPGSRAPHSIQSPCFPKEKG PPRLPLTPPPASAASPGAAWTGLPACWDMSGLQ GPSVGDGCNGGGARAGGSCCRRRCFRGFGRRVQG AAFPGRQPAELGPVPRGLPAAAAPVCPAASAAA AGGILASEHSRGPSAAGGHPGPAASEPVLPPLP GPQRVDLGPRTLALRATSASPLPRRATPGHTGCL SPGCPDQPARWSEWPGPSGPPGPELQQPGDTAG LCPADARSGCALAVSQLPL*AA*GSGGPPRPHL PHSDTQPPADAAPSTGGPIHPAAPRSLSESAGHA TS*DWRPGQPPGAQPGLQPAAEPPSLSG*VAPA PRHTGPTGGSLKPACLLQGPLHPQAWSTAASWL SLHLPGL*DSLSGFVRVL*AQLWRRV*NSCSGRR QQECPSAVGWAGAKPKPATARSGTSVLAAPCPA QQPPGLCAS*LGPPSTPHPARPEGQPAPGPAP* AARRPLCAPAGEPPG*GLARRPEFTSGSPHSRNA QTVPDLRFGQLSCDPSRLLSDPGLWRPPAVPSG SHRHPHHHPLSAAAAGARPRPPGSS*RPAQPCA GAAAPWGGLPAGCGAVAALHPTAGPALP*SGGQD PE*QQLG*PGDLPGGRGTPAALGSLPGAPLLLV PCGFPPCVQCLPGATGGDTAVLLGSSWGQSHLP PWGH*GASSSLHAGGAHGWPRAAGPPGRTRGCSE PPAVPVTERSPQLPPTGHRAAASALWHHRPQSG PLPPAPVVLGPSCSHLG*HHSSGGPGAHPPVRT LPGHTLLLVLALVHHQELCGRPGSEGLGAAAAAP CEPHRSAAAPGP*AGPAAVPAPKQGGRHPSAAA GAVPGPRAL*HGGDVRGRRVLCGLRARHRRGC* PP*LCGGQNLLCLLLAPEECEGGIRDHHSGPGGS GCAGPGVLLPWRGACAGARGG*GCPAEEGRRRP VDGHSAHQAAETSRVRGATAGGWPLLGTLESGR CRDRLSDAEQPAECGWASGSGLASRGPAPGGVLP GGAAHPARVPG*SG*ADPSHALLLG*APGWAAR GCGAPGAGPGAE*PAGRG*RGARSLGARPPQVP GQHPTHGLGPQGPRSAWLLGSTAPRACPGLGPTD F*AGPDIPQWMGRAPTFKSLQCVGTGPCEQQNC TVSFTSELDLFVG

Frame 1

PDARGIPSLPRGWGPPPTPSERCAVHHPPQWTE LERPGEVSSGAHELGSRPQDPEAAQWLTPQALT LTRPSLTFGAQSPPR*GRVTAFLVKRM*IEGGLI SRTGEQPL*PRSSTAPLARFPGPGLKPHYPGFA AHSEVSLPGAPASHGGPIPQPSFQW*PRPPGFR VTTRSPPLCSPGPDGLALELPTPSRVPVSPKRRA HPGSRSLLLLPLQLPRALPGQACLRAGTCLASK DRRWAMAATVEGPELEAAAAAGDASEDSDAGSR ALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLL QVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSL VLKGSIWDLGPWL*GPHPPPPFPGGQRRDTLGA CLRGALTNLPAGLSGLAHLAHLDLSFNSLETLPA CVLQMRGLGALLLSHNCLSELPEALGALPALTF LTVTHNRLQTLPPALGALSTLQRLDLSQNLLDT LPPEIGGLGSLLELNLASNRLQSLPASLGE*PLR PDTLAPREGP*SLPVFCRGLCTHRLGPQLPLGC PSTSLAFETPSVALSEFSEPSCGGESETAALGG GSRSVPAPWAGLVPSLSQQLPAAGLRSLRLLVLH SNLLASVPADLARLPLLTRLDLRDNQLRDLPPE LLDAPFVRLQGNPLGEASPDAPSSPVAALIPEM PRLFLTSDLDSFPVTPQGCSVTLACGVRLQFPAG ATATPITIRYRLLLPEPGLVPLGPHDALLSHVL ELQPHGVAFQQDVGLWLLFTPPQARRCREVVVR TRNDNSWGDLETYLEEEAPQRLWAHCQVPHFSWF LVVSRPVSNACLVPPEGTLLCSSGHPGVKVIFP PGATEEPRRVSMQVVRMAGRELQALLGEPEAAV SPLLCLSQSGPPSFLQPVTVQLPLPSGITGLSLD RSRLHLLYWAPPAATWDDITAQVVLELTHLYAR FQVTHFSWYWLWYTTKNCVGGLARKAWERLRLH RVNLIALQRRRDPEQVLLQCLPRNKVDATLRRLL ERYRGPEPSDTVEMFEGEEFFAAFERGIDVDAD RPDCVEGRICFVFYSHLKNVKEVYVTTTLDREA QAVRGQVSFYRGAVPVRVPEEAEAARQRKGADAL WMATLPIKLPRLRGSEGPRRGAGLSLAPLNLGD AETGFLTQSNLLSVAGRLGLDWPAVALHLGVSY REVQRIRHEFRDDLDEQIRHMLFSWAERQAGQPG AVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQ DSIRRMGLAPKDPALPGSSAPQPPEPAQA*APQ TFRLAQIFPSGWAEPPPSSLSSVWGRVPVSNKTA LFLSPQSLIYLLD

Frame 2

PTPGGSRACLGAGARPPRRLSGARCTTLPSGPN WKGRERFPPGLTNWGPGPRTPKLPNGSRPRPSP LHGLP*LSGRSHPRGRGGSRPSW*NGCK*KGDS SPGLGNSPYDLAAPRLHWPDFQDQD*SLTTPALL LIARSPCPVLLRAMAVPFPNPLSSGDPGLRGSG *PQGLPHSAAQAQMAWL*SSPLHPESLFPQREG PTPAPAHSSSCLCSFPGRCLDRPACVLGHVWPPR TVGGRWLQRWRGQSWRQLLPQEMLQRIRTQGPG RCLSWAATG*AWTCTPGAASSCCTCVSSSLCSC CRWNSCV*ALTRTLSCWRPPWPSCLRACPASAPW SSKGRSGTSDPGSEGHIRLPPSQEGNAGTHWVP VSGVP*PTCPLV*VAWPIWPTWT*ASTAWRHCR PVSCRCEVWVRSCCLTTASLSCLRLWGPSPPSPS SQ*HTTACRRCPQHWGPYPPCSASISLRICWTR YLLRLEAWAASWSSTWPPTGCRASQPLWVSSPC APTHWPHGRVPEACLSSAGASAPTGLVHSCLLVV PPPPWPLRLPQWLCQSSLSPAVEESLKQLLWEA AAGVSQRRGLGWCQA*ASNCPQRDFGPCGSLSC TATSWPLCQLTWPAFHSSPGST*GTTSSGTCPLS C*TPPLCACRGTPWVRPRQTPRVHQWQPSFQKC PDCS*PQIWTAFL*PLKAAQ*PWPVASACSSQR EPPPPPSPSAIGCCCRSQASSPWVLMTPCSAMCW SCSPMGWPSSRMWGCGCSSPHRRPGAAVKWWSG PGMTTAGVTWRPTWRKRHPSGSGLTARCPTSPG SLWFPALCPMPAWCHRRGHCCAPRVILGSKSSSP LGPLRSLVESPCRWCAWLAESCRPSWENQRLQ* APCCACHRAVPPASSNRSPCSCLCPLASQASVW TAPACTCCTGPLLQPPGMTSQLRWSWSSPTCTHA SRSHTSPGTGSGTPPRTVWEAWLGRPGSGCGCT V*TSSLCSGAGTLSRSCCSACPETRWTPPFGGC WSGTGAPSPLTRWRCSRAKSSLRPSSAASTWMLT ALTVWRAESALSSTRT*RM*RRYT*PPLWTGRL RLCGARCPSTVARCLCGCPRRLRLPGRGRAQTP CGWPLCPSSCRDFEGPRGHGGGLASPWHP*IWEM PRPAF*RRATC*VWLGVWVWTGQPWPCTWGCPT GRCSASGTSSGMIWMSRSVTCSSPGLSARLGSQ GLWGSWCRPWSRVTGRTWLKRCAQSWSSAAASTR TASDAWAWPPRTPLCLAPRLHSPQSLPRPRPHR LLGWPRYSPVDGQSPHLQVSPVCGDGSL*ATKL HCFFHLRA*FICWM

Sequence LRDD.dAug05

CCCCGACGCCCGGGGGATCCCGAGCCTGCCTCGGGGCTGGGGCCCGCCCCCCACGCCGTCTGAGCGGTGCGCGGTGCACCACCCTCCCCAGTGGACCGAA CTGGAAAGGCCGGGAGAGGTTTCCTCCGGGGCTCACGAATTGGGGTCCCGGCCCCAGGACCCCGAAGCTGCCCAATGGCTCACGCCCCAGGCCCTCACCC TTACACGGCCTTCCCTGACTTTCGGGGCGCAGTCACCCCCGCGGTAGGGGAGGGTCACGGCCTTCCTGGTAAAACGGATGTAAATAGAAGGGGGACTCAT CTCCAGGACTGGGGAACAGCCCCTATGACCTCGCAGCTCCACGGCTCCATTGGCCAGATTTCCAGGACCAGGACTGAAGCCTCACTACCCCGGCTTTGCT GCTCATAGCGAGGTCTCCCTGCCCGGTGCTCCTGCGAGCCATGGCGGTCCCATTCCCCAACCCTCTTTCCAGTGGTGACCCAGGCCTCCGGGGTTCAGGG TGACCACAAGGTCTCCCCCACTCTGCAGCCCAGGCCCAGATGGCCTGGCTCTAGAGCTCCCCACTCCATCCAGAGTCCCTGTTTCCCCAAAGAGAAGGGC CCACCCCGGCTCCCGCTCACTCCTCCTCCTGCCTCTGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAG GACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGG CGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCT GCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTG GTCCTCAAAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCT GTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGC CTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTC CTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGC TACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGGGACTTCGGTCCTT GCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTC CGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAG CCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCG CCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCC CTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCC GTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGGCTCACTGCCAGGT GCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGG GTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAG AACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCAT CACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTC ACCCACCTGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGACCGGGAGGCTCAGGCTGTGCGGGGCC AGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCT GCCCATCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTT CTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCA TCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCT GGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGC ATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCC AGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACC TCAGAGCTTGATTTATTTGTTGGATG

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

PRRPGDPEPASGLGPAPHAV*AVRGAPPSPVDR TGKAGRGFLRGSRIGVPAPGPRSCPMAHAPGPHP YTAFPDFRGAVTPAVGEGHGLPGKTDVNRRGTH LQDWGTAPMTSQLHGSIGQISRTRTEASLPRLC CS*RGLPARCSCEPWRSHSPTLFPVVTQASGVQG DHKVSPTLQPRPRWPGSRAPHSIQSPCFPKEKG PPRLPLTPPPASAASPGAAWTGLPACWDMSGLQ GPSVGDGCNGGGARAGGSCCRRRCFRGFGRRVQG AAFPGRQPAELGPVPRGLPAAAAPVCPAASAAA AGGILASEHSRGPSAAGGHPGPAASEPVLPPLP GPQRVDLGPRTLALRATSASPLPRRATPGHTGCL SPGCPDQPARWSEWPGPSGPPGPELQQPGDTAG LCPADARSGCALAVSQLPL*AA*GSGGPPRPHL PHSDTQPPADAAPSTGGPIHPAAPRSLSESAGHA TS*DWRPGQPPGAQPGLQPAAEPPSLSGGTSVL AAPCPAQQPPGLCAS*LGPPSTPHPARPEGQPA PGPAP*AARRPLCAPAGEPPG*GLARRPEFTSGS PHSRNAQTVPDLRFGQLSCDPSRLLSDPGLWRP PAVPSGSHRHPHHHPLSAAAAGARPRPPGSS*R PAQPCAGAAAPWGGLPAGCGAVAALHPTAGPALP *SGGQDPE*QQLG*PGDLPGGRGTPAALGSLPG APLLLVPCGFPPCVQCLPGATGGDTAVLLGSSW GQSHLPPWGH*GASSSLHAGGAHGWPRAAGPPGR TRGCSEPPAVPVTERSPQLPPTGHRAAASALWH HRPQSGPLPPAPVVLGPSCSHLG*HHSSGGPGA HPPVLALVHHQELCGRPGSEGLGAAAAAPCEPHR SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC GGQNLLCLLLAPEECEGGIRDHHSGPGGSGCAGP GVLLPWRGACAGARGG*GCPAEEGRRRPVDGHS AHQAAETSRVRGATAGGWPLLGTLESGRCRDRL SDAEQPAECGWASGSGLASRGPAPGGVLPGGAAH PARVPG*SG*ADPSHALLLG*APGWAARGCGAP GAGPGAE*PAGRG*RGARSLGARPPQVPGQHPT HGLGPQGPRSAWLLGSTAPRACPGLGPTDF*AGP DIPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFT SELDLFVG

Frame 1

PDARGIPSLPRGWGPPPTPSERCAVHHPPQWTE LERPGEVSSGAHELGSRPQDPEAAQWLTPQALT LTRPSLTFGAQSPPR*GRVTAFLVKRM*IEGGLI SRTGEQPL*PRSSTAPLARFPGPGLKPHYPGFA AHSEVSLPGAPASHGGPIPQPSFQW*PRPPGFR VTTRSPPLCSPGPDGLALELPTPSRVPVSPKRRA HPGSRSLLLLPLQLPRALPGQACLRAGTCLASK DRRWAMAATVEGPELEAAAAAGDASEDSDAGSR ALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLL QVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSL VLKGSIWDLGPWL*GPHPPPPFPGGQRRDTLGA CLRGALTNLPAGLSGLAHLAHLDLSFNSLETLPA CVLQMRGLGALLLSHNCLSELPEALGALPALTF LTVTHNRLQTLPPALGALSTLQRLDLSQNLLDT LPPEIGGLGSLLELNLASNRLQSLPASLAGLRSL RLLVLHSNLLASVPADLARLPLLTRLDLRDNQL RDLPPELLDAPFVRLQGNPLGEASPDAPSSPVA ALIPEMPRLFLTSDLDSFPVTPQGCSVTLACGVR LQFPAGATATPITIRYRLLLPEPGLVPLGPHDA LLSHVLELQPHGVAFQQDVGLWLLFTPPQARRC REVVVRTRNDNSWGDLETYLEEEAPQRLWAHCQV PHFSWFLVVSRPVSNACLVPPEGTLLCSSGHPG VKVIFPPGATEEPRRVSMQVVRMAGRELQALLG EPEAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGI TGLSLDRSRLHLLYWAPPAATWDDITAQVVLEL THLYWLWYTTKNCVGGLARKAWERLRLHRVNLI ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV EGRICFVFYSHLKNVKEVYVTTTLDREAQAVRG QVSFYRGAVPVRVPEEAEAARQRKGADALWMATL PIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGF LTQSNLLSVAGRLGLDWPAVALHLGVSYREVQR IRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLL VQALEQSDRQDVAEEVRAVLELGRRKYQDSIRR MGLAPKDPALPGSSAPQPPEPAQA*APQTFRLA QIFPSGWAEPPPSSLSSVWGRVPVSNKTALFLSP QSLIYLLD

Frame 2

PTPGGSRACLGAGARPPRRLSGARCTTLPSGPN WKGRERFPPGLTNWGPGPRTPKLPNGSRPRPSP LHGLP*LSGRSHPRGRGGSRPSW*NGCK*KGDS SPGLGNSPYDLAAPRLHWPDFQDQD*SLTTPALL LIARSPCPVLLRAMAVPFPNPLSSGDPGLRGSG *PQGLPHSAAQAQMAWL*SSPLHPESLFPQREG PTPAPAHSSSCLCSFPGRCLDRPACVLGHVWPPR TVGGRWLQRWRGQSWRQLLPQEMLQRIRTQGPG RCLSWAATG*AWTCTPGAASSCCTCVSSSLCSC CRWNSCV*ALTRTLSCWRPPWPSCLRACPASAPW SSKGRSGTSDPGSEGHIRLPPSQEGNAGTHWVP VSGVP*PTCPLV*VAWPIWPTWT*ASTAWRHCR PVSCRCEVWVRSCCLTTASLSCLRLWGPSPPSPS SQ*HTTACRRCPQHWGPYPPCSASISLRICWTR YLLRLEAWAASWSSTWPPTGCRASQPLWRDFGP CGSLSCTATSWPLCQLTWPAFHSSPGST*GTTSS GTCPLSC*TPPLCACRGTPWVRPRQTPRVHQWQ PSFQKCPDCS*PQIWTAFL*PLKAAQ*PWPVAS ACSSQREPPPPPSPSAIGCCCRSQASSPWVLMTP CSAMCWSCSPMGWPSSRMWGCGCSSPHRRPGAA VKWWSGPGMTTAGVTWRPTWRKRHPSGSGLTAR CPTSPGSLWFPALCPMPAWCHRRGHCCAPRVILG SKSSSPLGPLRSLVESPCRWCAWLAESCRPSWE NQRLQ*APCCACHRAVPPASSNRSPCSCLCPLA SQASVWTAPACTCCTGPLLQPPGMTSQLRWSWSS PTCTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW RAESALSSTRT*RM*RRYT*PPLWTGRLRLCGA RCPSTVARCLCGCPRRLRLPGRGRAQTPCGWPL CPSSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF *RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSA SGTSSGMIWMSRSVTCSSPGLSARLGSQGLWGS WCRPWSRVTGRTWLKRCAQSWSSAAASTRTASDA WAWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWP RYSPVDGQSPHLQVSPVCGDGSL*ATKLHCFFH LRA*FICWM

Sequence LRDD.cAug05

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA SEPVLPPLPGPQRVDLGPRTLALRATSASPLPRR ATPGHTGCLSPGCPDQPARWSEWPGPSGPPGPE LQQPGDTAGLCPADARSGCALAVSQLPL*AA*G SGGPPRPHLPHSDTQPPADAAPSTGGPIHPAAPR SLSESAGHATS*DWRPGQPPGAQPGLQPAAEPP SLSGGTSVLAAPCPAQQPPGLCAS*LGPPSTPH PARPEGQPAPGPAP*AARRPLCAPAGEPPG*GLA RRPEFTSGSPHSRNAQTVPDLRFGQLSCDPSRL LSDPGLWRPPAVPSGSHRHPHHHPLSAAAAGAR PRPPGSS*RPAQPCAGAAAPWGGLPAGCGAVAAL HPTAGPALP*SGGQDPE*QQLG*PGDLPGGRGT PAALGSLPGAPLLLVPCGFPPCVQCLPGATGGD TAVLLGSSWGQSHLPPWGH*GASSSLHAGGAHGW PRAAGPPGRTRGCSEPPAVPVTERSPQLPPTGH RAAASALWHHRPQSGPLPPAPVVLGPSCSHLG* HHSSGGPGAHPPVLALVHHQELCGRPGSEGLGAA AAAPCEPHRSAAAPGP*AGPAAVPAPKQGGRHP SAAAGAVPGPRAL*HGGDVRGRRVLCGLRARHR RGC*PP*LCGGQNLLCLLLAPEECEGGIRDHHSG PGGSGCAGPGVLLPWRGACAGARGG*GCPAEEG RRRPVDGHSAHQAAETSRVRGATAGGWPLLGTL ESGRCRDRLSDAEQPAECGWASGSGLASRGPAPG GVLPGGAAHPARVPG*SG*ADPSHALLLG*APG WAARGCGAPGAGPGAE*PAGRG*RGARSLGARP PQVPGQHPTHGLGPQGPRSAWLLGSTAPRACPGL GPTDF*AGPDIPQWMGRAPTFKSLQCVGTGPCE QQNCTVSFTSELDLFVG

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP QSLSCLRSLVLKGSIWDLGPWL*GPHPPPPFPG GQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLS FNSLETLPACVLQMRGLGALLLSHNCLSELPEA LGALPALTFLTVTHNRLQTLPPALGALSTLQRL DLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLP ASLAGLRSLRLLVLHSNLLASVPADLARLPLLT RLDLRDNQLRDLPPELLDAPFVRLQGNPLGEAS PDAPSSPVAALIPEMPRLFLTSDLDSFPVTPQGC SVTLACGVRLQFPAGATATPITIRYRLLLPEPG LVPLGPHDALLSHVLELQPHGVAFQQDVGLWLL FTPPQARRCREVVVRTRNDNSWGDLETYLEEEAP QRLWAHCQVPHFSWFLVVSRPVSNACLVPPEGT LLCSSGHPGVKVIFPPGATEEPRRVSMQVVRMA GRELQALLGEPEAAVSPLLCLSQSGPPSFLQPVT VQLPLPSGITGLSLDRSRLHLLYWAPPAATWDD ITAQVVLELTHLYWLWYTTKNCVGGLARKAWER LRLHRVNLIALQRRRDPEQVLLQCLPRNKVDATL RRLLERYRGPEPSDTVEMFEGEEFFAAFERGID VDADRPDCVEGRICFVFYSHLKNVKEVYVTTTL DREAQAVRGQVSFYRGAVPVRVPEEAEAARQRKG ADALWMATLPIKLPRLRGSEGPRRGAGLSLAPL NLGDAETGFLTQSNLLSVAGRLGLDWPAVALHL GVSYREVQRIRHEFRDDLDEQIRHMLFSWAERQA GQPGAVGLLVQALEQSDRQDVAEEVRAVLELGR RKYQDSIRRMGLAPKDPALPGSSAPQPPEPAQA *APQTFRLAQIFPSGWAEPPPSSLSSVWGRVPVS NKTALFLSPQSLIYLLD

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL RACPASAPWSSKGRSGTSDPGSEGHIRLPPSQE GNAGTHWVPVSGVP*PTCPLV*VAWPIWPTWT* ASTAWRHCRPVSCRCEVWVRSCCLTTASLSCLRL WGPSPPSPSSQ*HTTACRRCPQHWGPYPPCSAS ISLRICWTRYLLRLEAWAASWSSTWPPTGCRAS QPLWRDFGPCGSLSCTATSWPLCQLTWPAFHSSP GST*GTTSSGTCPLSC*TPPLCACRGTPWVRPR QTPRVHQWQPSFQKCPDCS*PQIWTAFL*PLKA AQ*PWPVASACSSQREPPPPPSPSAIGCCCRSQA SSPWVLMTPCSAMCWSCSPMGWPSSRMWGCGCS SPHRRPGAAVKWWSGPGMTTAGVTWRPTWRKRH PSGSGLTARCPTSPGSLWFPALCPMPAWCHRRGH CCAPRVILGSKSSSPLGPLRSLVESPCRWCAWL AESCRPSWENQRLQ*APCCACHRAVPPASSNRS PCSCLCPLASQASVWTAPACTCCTGPLLQPPGMT SQLRWSWSSPTCTGSGTPPRTVWEAWLGRPGSG CGCTV*TSSLCSGAGTLSRSCCSACPETRWTPP FGGCWSGTGAPSPLTRWRCSRAKSSLRPSSAAST WMLTALTVWRAESALSSTRT*RM*RRYT*PPLW TGRLRLCGARCPSTVARCLCGCPRRLRLPGRGR AQTPCGWPLCPSSCRDFEGPRGHGGGLASPWHP* IWEMPRPAF*RRATC*VWLGVWVWTGQPWPCTW GCPTGRCSASGTSSGMIWMSRSVTCSSPGLSAR LGSQGLWGSWCRPWSRVTGRTWLKRCAQSWSSAA ASTRTASDAWAWPPRTPLCLAPRLHSPQSLPRP RPHRLLGWPRYSPVDGQSPHLQVSPVCGDGSL* ATKLHCFFHLRA*FICWM

Sequence LRDD.gAug05

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGTGAGTTCCACCTGTTCTGGGCCCTGTCTCCATCTCTCGCTCTGCCCTGCCTCTGCTGCATGT TCTCAAAGGCAAACCCCAGTCCCAGCCCCAGCTCCAGGCCGCCCCTCCCGGCTCCCCAGGTGTGAACTGTTCTGCTCTTGGTCCTGTCCAGCCCCATCGC CATCCTCGGTGATCACCACCCCCAGTGTGGGGCTGGCTGGGCCCTGGCTCCTGGACCCTGACGCTGCCCAGTGGGGGTCTCTCCAGACCACAGTGCAGAT GTTGTTCCTGGGGAAGGTTGTGGGTGATGGTTGTCAGCTGAGATGAGCCGGTGGTCTCCACCCCTGCCTCCTTCCATTTGCGGGCACCTCTCCAGTGTTT CTGTCTGTCTGTCCCTGTCTGGCTGGGGTGGGGGTGGGGTGGGAGGTCTGTGCCTCTCGAGCAGCTGTTTCTCTGGCTGGCAGGGTCGATCTGGGACCTC GGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGC CCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGG TGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAG ACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCA GCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCGCCCCGACACACTGGCCCCACGGGAGGG TCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTCTGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGTCCCTCCACCTCCCTGGCCTTTGAGACT CCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCAGCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCGGCAGCAGGAGTGTCCCAGCGCCGTGGG CTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCCCGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGC TGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTG CAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGG ACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCG CTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTC CAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACC TGGAGACCTACCTGGAGGAAGAGGCACCCCAGGTGAGGGCCACCCAGGCCTGCCGGTGGCGAGTGGAGAGCTGCTGCCCTGAGCCGTGCACCTCTGCCCA GAGCCTCACCCTGGCACCTTCCACCCTGCCCCGTCCCTCCTGGATCCTGCTTCCCTATGTCCCTGGCACCTTCCACCCCACCCCGTCCCTCCTGGATCCT GCTTCCCTGTGTCCCTGGCAACTTCCACCCCACCCCGTCCCTCCTGGATCCTGCTCCCTGTGTCCCTGGCACCTTCCACCCCGCCCCGTCTCTCCTGGAT CCTGCTCCCTGTGTCCCTGACTGGCTGTGCCCTGACCCAGGCTCCTGTGACCTCCTCTCTCCCCCATCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCC ACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAA AGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCA GAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAG GCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCA CCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTCAGTGCCCCCCAGCTTTCTCAGCCCCCCTCCCCCAGTCTGTACAGCCCTCCTCACCCCCAGC TCTCCCAGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTG AGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGGTGGGACTGAGGGACAGCAGAGGGGCGGGGCAGGA CCGAGGCCCAGGGGTGACCAGGGTGACATGGTGGAGTTGGGGGTGGAGCCCAGGGCTTAATGCACTTTTTCCTTCCAACAGAGACTTCGAGGGTCCGAGG GGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCG TCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGTGAGGCTCCCACTGCCCG CTGGCTGCCAGCCCCAGGGCTGAGCCCTGGCTGCCTGGAGGGCCCACCTGATTCTGACAGAAGTTTCTTCCAGGGGAGGTGGGGGAAGGGCAAACACCAG GCTGGAAAGGAAAGGTCTGGCCGCACCTGGCCCCAGGAGCCGGGAACTCCCCCACACTCCGAGGCAGAGTGTGGTGGGGGGTCCTGGGCAGGTGGACAGG ACCCCTCTGTCGCCCATCCTCTGCCTGCAGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGG GCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGG ACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGAC TTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCA CTGTTTCTTTC

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA SEPVLPPLPGPQR*VPPVLGPVSISRSALPLLHV LKGKPQSQPQLQAAPPGSPGVNCSALGPVQPHR HPR*SPPPVWGWLGPGSWTLTLPSGGLSRPQCR CCSWGRLWVMVVS*DEPVVSTPASFHLRAPLQCF CLSVPVWLGWGWGGRSVPLEQLFLWLAGSIWDL GPWL*GPHPPPPFPGGQRRDTLGACLRGALTNL PAGLSGLAHLAHLDLSFNSLETLPACVLQMRGLG ALLLSHNCLSELPEALGALPALTFLTVTHNRLQ TLPPALGALSTLQRLDLSQNLLDTLPPEIGGLG SLLELNLASNRLQSLPASLGE*PLRPDTLAPREG P*SLPVFCRGLCTHRLGPQLPLGCPSTSLAFET PSVALSEFSEPSCGGESETAALGGGSRSVPAPW AGLVPSLSQQLPAAGLRSLRLLVLHSNLLASVPA DLARLPLLTRLDLRDNQLRDLPPELLDAPFVRL QGNPLGEASPDAPSSPVAALIPEMPRLFLTSDL DSFPVTPQGCSVTLACGVRLQFPAGATATPITIR YRLLLPEPGLVPLGPHDALLSHVLELQPHGVAF QQDVGLWLLFTPPQARRCREVVVRTRNDNSWGD LETYLEEEAPQVRATQACRWRVESCCPEPCTSAQ SLTLAPSTLPRPSWILLPYVPGTFHPTPSLLDP ASLCPWQLPPHPVPPGSCSLCPWHLPPRPVSPG SCSLCP*LAVP*PRLL*PPLSPIPAALGSLPGAP LLLVPCGFPPCVQCLPGATGGDTAVLLGSSWGQ SHLPPWGH*GASSSLHAGGAHGWPRAAGPPGRT RGCSEPPAVPVTERSPQLPPTGHRAAASALWHHR PQSGPLPPAPVVLGPSCSHLG*HHSSGGPGAHP PVRTLPGHTLLLVSAPQLSQPPSPSLYSPPHPQ LSQVLALVHHQELCGRPGSEGLGAAAAAPCEPHR SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC GGQNLLCLLLAPEECEGGVLLPWRGACAGARGG* GCPAEEGRRRPVDGHSAHQAAGGTEGQQRGGAG PRPRGDQGDMVELGVEPRA*CTFSFQQRLRGSE GPRRGAGLSLAPLNLGDAETGFLTQSNLLSVAGR LGLDWPAVALHLGVSYREVQRIRHEFR*GSHCP LAASPRAEPWLPGGPT*F*QKFLPGEVGEGQTP GWKGKVWPHLAPGAGNSPTLRGRVWWGVLGRWTG PLCRPSSACRDDLDEQIRHMLFSWAERQAGQPG AVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQ DSIRRMGLAPKDPALPGSSAPQPPEPAQA*APQT FRLAQIFPSGWAEPPPSSLSSVWGRVPVSNKTA LFL

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP QSLSCLRSLVLKGEFHLFWALSPSLALPCLCCM FSKANPSPSPSSRPPLPAPQV*TVLLLVLSSPIA ILGDHHPQCGAGWALAPGP*RCPVGVSPDHSAD VVPGEGCG*WLSAEMSRWSPPLPPSICGHLSSV SVCLSLSGWGGGGVGGLCLSSSCFSGWQGRSGTS DPGSEGHIRLPPSQEGNAGTHWVPVSGVP*PTC PLV*VAWPIWPTWT*ASTAWRHCRPVSCRCEVW VRSCCLTTASLSCLRLWGPSPPSPSSQ*HTTACR RCPQHWGPYPPCSASISLRICWTRYLLRLEAWA ASWSSTWPPTGCRASQPLWVSSPCAPTHWPHGR VPEACLSSAGASAPTGLVHSCLLVVPPPPWPLRL PQWLCQSSLSPAVEESLKQLLWEAAAGVSQRRG LGWCQA*ASNCPQRDFGPCGSLSCTATSWPLCQ LTWPAFHSSPGST*GTTSSGTCPLSC*TPPLCAC RGTPWVRPRQTPRVHQWQPSFQKCPDCS*PQIW TAFL*PLKAAQ*PWPVASACSSQREPPPPPSPS AIGCCCRSQASSPWVLMTPCSAMCWSCSPMGWPS SRMWGCGCSSPHRRPGAAVKWWSGPGMTTAGVT WRPTWRKRHPR*GPPRPAGGEWRAAALSRAPLP RASPWHLPPCPVPPGSCFPMSLAPSTPPRPSWIL LPCVPGNFHPTPSLLDPAPCVPGTFHPAPSLLD PAPCVPDWLCPDPGSCDLLSPPSQRLWAHCQVP HFSWFLVVSRPVSNACLVPPEGTLLCSSGHPGVK VIFPPGATEEPRRVSMQVVRMAGRELQALLGEP EAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGIT GLSLDRSRLHLLYWAPPAATWDDITAQVVLELTH LYARFQVTHFSWSVPPSFLSPPPPVCTALLTPS SPRYWLWYTTKNCVGGLARKAWERLRLHRVNLI ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV EGRICFVFYSHLKNVKEVSFYRGAVPVRVPEEA EAARQRKGADALWMATLPIKLPVGLRDSRGAGQD RGPGVTRVTWWSWGWSPGLNALFPSNRDFEGPR GHGGGLASPWHP*IWEMPRPAF*RRATC*VWLG VWVWTGQPWPCTWGCPTGRCSASGTSSGEAPTAR WLPAPGLSPGCLEGPPDSDRSFFQGRWGKGKHQ AGKERSGRTWPQEPGTPPHSEAECGGGSWAGGQ DPSVAHPLPAGMIWMSRSVTCSSPGLSARLGSQG LWGSWCRPWSRVTGRTWLKRCAQSWSSAAASTR TASDAWAWPPRTPLCLAPRLHSPQSLPRPRPHR LLGWPRYSPVDGQSPHLQVSPVCGDGSL*ATKLH CFF

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL RACPASAPWSSKVSSTCSGPCLHLSLCPASAAC SQRQTPVPAPAPGRPSRLPRCELFCSWSCPAPS PSSVITTPSVGLAGPWLLDPDAAQWGSLQTTVQM LFLGKVVGDGCQLR*AGGLHPCLLPFAGTSPVF LSVCPCLAGVGVGWEVCASRAAVSLAGRVDLGP RTLALRATSASPLPRRATPGHTGCLSPGCPDQPA RWSEWPGPSGPPGPELQQPGDTAGLCPADARSG CALAVSQLPL*AA*GSGGPPRPHLPHSDTQPPA DAAPSTGGPIHPAAPRSLSESAGHATS*DWRPGQ PPGAQPGLQPAAEPPSLSG*VAPAPRHTGPTGG SLKPACLLQGPLHPQAWSTAASWLSLHLPGL*D SLSGFVRVL*AQLWRRV*NSCSGRRQQECPSAVG WAGAKPKPATARSGTSVLAAPCPAQQPPGLCAS *LGPPSTPHPARPEGQPAPGPAP*AARRPLCAP AGEPPG*GLARRPEFTSGSPHSRNAQTVPDLRFG QLSCDPSRLLSDPGLWRPPAVPSGSHRHPHHHP LSAAAAGARPRPPGSS*RPAQPCAGAAAPWGGL PAGCGAVAALHPTAGPALP*SGGQDPE*QQLG*P GDLPGGRGTPGEGHPGLPVASGELLP*AVHLCP EPHPGTFHPAPSLLDPASLCPWHLPPHPVPPGS CFPVSLATSTPPRPSWILLPVSLAPSTPPRLSWI LLPVSLTGCALTQAPVTSSLPHPSGSGLTARCP TSPGSLWFPALCPMPAWCHRRGHCCAPRVILGS KSSSPLGPLRSLVESPCRWCAWLAESCRPSWENQ RLQ*APCCACHRAVPPASSNRSPCSCLCPLASQ ASVWTAPACTCCTGPLLQPPGMTSQLRWSWSSP TCTHASRSHTSPGQCPPAFSAPLPQSVQPSSPPA LPGTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW RAESALSSTRT*RM*RRCPSTVARCLCGCPRRL RLPGRGRAQTPCGWPLCPSSCRWD*GTAEGRGR TEAQG*PG*HGGVGGGAQGLMHFFLPTETSRVRG ATAGGWPLLGTLESGRCRDRLSDAEQPAECGWA SGSGLASRGPAPGGVLPGGAAHPARVPVRLPLP AGCQPQG*ALAAWRAHLILTEVSSRGGGGRANTR LERKGLAAPGPRSRELPHTPRQSVVGGPGQVDR TPLSPILCLQG*SG*ADPSHALLLG*APGWAAR GCGAPGAGPGAE*PAGRG*RGARSLGARPPQVPG QHPTHGLGPQGPRSAWLLGSTAPRACPGLGPTD F*AGPDIPQWMGRAPTFKSLQCVGTGPCEQQNC TVSF

Sequence LRDD.fAug05

GCCTGCGCCGCGGGCCTACCCGGAACGGCGCGTGGGCCCGGGCAGAAGCTGCTGGCGGTAGGCTCGCCTTGACTCCTTGGTGGTTCCCTGGCCGCGGCGA CCGGTGGTCCCACGTCCAGCCGGGCTCAGTGAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTC GGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCC TTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTG GAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCA AAGGTGAGTTCCACCTGTTCTGGGCCCTGTCTCCATCTCTCGCTCTGCCCTGCCTCTGCTGCATGTTCTCAAAGGCAAACCCCAGTCCCAGCCCCAGCTC CAGGCCGCCCCTCCCGGCTCCCCAGGTGTGAACTGTTCTGCTCTTGGTCCTGTCCAGCCCCATCGCCATCCTCGGTGATCACCACCCCCAGTGTGGGGCT GGCTGGGCCCTGGCTCCTGGACCCTGACGCTGCCCAGTGGGGGTCTCTCCAGACCACAGTGCAGATGTTGTTCCTGGGGAAGGTTGTGGGTGATGGTTGT CAGCTGAGATGAGCCGGTGGTCTCCACCCCTGCCTCCTTCCATTTGCGGGCACCTCTCCAGTGTTTCTGTCTGTCTGTCCCTGTCTGGCTGGGGTGGGGG TGGGGTGGGAGGTCTGTGCCTCTCGAGCAGCTGTTTCTCTGGCTGGCAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCC CCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCA CCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAG CTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCC TGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCT GCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCGCCCCGACACACTGGCCCCACGGGAGGGTCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTC TGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGTCCCTCCACCTCCCTGGCCTTTGAGACTCCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCA GCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCGGCAGCAGGAGTGTCCCAGCGCCGTGGGCTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCC CGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTC GACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACG CCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGT GACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTC CCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCC CACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCG GCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTG TGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAG AGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCA GCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACA GCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTCAGTGCCCCCCAGCTTTCTCAGCCCCCCTCCCCCAG TCTGTACAGCCCTCCTCACCCCCAGCTCTCCCAGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCT GCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTT CGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACG TGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGA CCGGGAGGCTCAGGCTGTGCGGGGCCAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGC GCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGGTGGGACTGAGGGACAGCAGAGGGGCGGGGCAGGACCGAGGCCCAGGGGTGACCAGGG TGACATGGTGGAGTTGGGGGTGGAGCCCAGGGCTTAATGCACTTTTTCCTTCCAACAGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTC TCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCG TGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTG GGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTC TTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAG AGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGG ACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACCTCAGAGCTTGATTTATTTGTTGGATG

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA TGGPTSSRAQ*ASPGAAWTGLPACWDMSGLQGPS VGDGCNGGGARAGGSCCRRRCFRGFGRRVQGAA FPGRQPAELGPVPRGLPAAAAPVCPAASAAAAG GILASEHSRGPSAAGGHPGPAASEPVLPPLPGPQ R*VPPVLGPVSISRSALPLLHVLKGKPQSQPQL QAAPPGSPGVNCSALGPVQPHRHPR*SPPPVWG WLGPGSWTLTLPSGGLSRPQCRCCSWGRLWVMVV S*DEPVVSTPASFHLRAPLQCFCLSVPVWLGWG WGGRSVPLEQLFLWLAGSIWDLGPWL*GPHPPP PFPGGQRRDTLGACLRGALTNLPAGLSGLAHLAH LDLSFNSLETLPACVLQMRGLGALLLSHNCLSE LPEALGALPALTFLTVTHNRLQTLPPALGALST LQRLDLSQNLLDTLPPEIGGLGSLLELNLASNRL QSLPASLGE*PLRPDTLAPREGP*SLPVFCRGL CTHRLGPQLPLGCPSTSLAFETPSVALSEFSEP SCGGESETAALGGGSRSVPAPWAGLVPSLSQQLP AAGLRSLRLLVLHSNLLASVPADLARLPLLTRL DLRDNQLRDLPPELLDAPFVRLQGNPLGEASPD APSSPVAALIPEMPRLFLTSDLDSFPVTPQGCSV TLACGVRLQFPAGATATPITIRYRLLLPEPGLV PLGPHDALLSHVLELQPHGVAFQQDVGLWLLFT PPQARRCREVVVRTRNDNSWGDLETYLEEEAPQR LWAHCQVPHFSWFLVVSRPVSNACLVPPEGTLL CSSGHPGVKVIFPPGATEEPRRVSMQVVRMAGR ELQALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQ LPLPSGITGLSLDRSRLHLLYWAPPAATWDDIT AQVVLELTHLYARFQVTHFSWSVPPSFLSPPPP VCTALLTPSSPRYWLWYTTKNCVGGLARKAWERL RLHRVNLIALQRRRDPEQVLLQCLPRNKVDATL RRLLERYRGPEPSDTVEMFEGEEFFAAFERGID VDADRPDCVEGRICFVFYSHLKNVKEVYVTTTLD REAQAVRGQVSFYRGAVPVRVPEEAEAARQRKG ADALWMATLPIKLPVGLRDSRGAGQDRGPGVTR VTWWSWGWSPGLNALFPSNRDFEGPRGHGGGLAS PWHP*IWEMPRPAF*RRATC*VWLGVWVWTGQP WPCTWGCPTGRCSASGTSSGMIWMSRSVTCSSP GLSARLGSQGLWGSWCRPWSRVTGRTWLKRCAQS WSSAAASTRTASDAWAWPPRTPLCLAPRLHSPQ SLPRPRPHRLLGWPRYSPVDGQSPHLQVSPVCG DGSL*ATKLHCFFHLRA*FICWM

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR PVVPRPAGLSELPRALPGQACLRAGTCLASKDR RWAMAATVEGPELEAAAAAGDASEDSDAGSRALP FLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQV EFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVL KGEFHLFWALSPSLALPCLCCMFSKANPSPSPSS RPPLPAPQV*TVLLLVLSSPIAILGDHHPQCGA GWALAPGP*RCPVGVSPDHSADVVPGEGCG*WL SAEMSRWSPPLPPSICGHLSSVSVCLSLSGWGGG GVGGLCLSSSCFSGWQGRSGTSDPGSEGHIRLP PSQEGNAGTHWVPVSGVP*PTCPLV*VAWPIWP TWT*ASTAWRHCRPVSCRCEVWVRSCCLTTASLS CLRLWGPSPPSPSSQ*HTTACRRCPQHWGPYPP CSASISLRICWTRYLLRLEAWAASWSSTWPPTG CRASQPLWVSSPCAPTHWPHGRVPEACLSSAGAS APTGLVHSCLLVVPPPPWPLRLPQWLCQSSLSP AVEESLKQLLWEAAAGVSQRRGLGWCQA*ASNC PQRDFGPCGSLSCTATSWPLCQLTWPAFHSSPGS T*GTTSSGTCPLSC*TPPLCACRGTPWVRPRQT PRVHQWQPSFQKCPDCS*PQIWTAFL*PLKAAQ *PWPVASACSSQREPPPPPSPSAIGCCCRSQASS PWVLMTPCSAMCWSCSPMGWPSSRMWGCGCSSP HRRPGAAVKWWSGPGMTTAGVTWRPTWRKRHPS GSGLTARCPTSPGSLWFPALCPMPAWCHRRGHCC APRVILGSKSSSPLGPLRSLVESPCRWCAWLAE SCRPSWENQRLQ*APCCACHRAVPPASSNRSPC SCLCPLASQASVWTAPACTCCTGPLLQPPGMTSQ LRWSWSSPTCTHASRSHTSPGQCPPAFSAPLPQ SVQPSSPPALPGTGSGTPPRTVWEAWLGRPGSG CGCTV*TSSLCSGAGTLSRSCCSACPETRWTPPF GGCWSGTGAPSPLTRWRCSRAKSSLRPSSAAST WMLTALTVWRAESALSSTRT*RM*RRYT*PPLW TGRLRLCGARCPSTVARCLCGCPRRLRLPGRGRA QTPCGWPLCPSSCRWD*GTAEGRGRTEAQG*PG *HGGVGGGAQGLMHFFLPTETSRVRGATAGGWP LLGTLESGRCRDRLSDAEQPAECGWASGSGLASR GPAPGGVLPGGAAHPARVPG*SG*ADPSHALLL G*APGWAARGCGAPGAGPGAE*PAGRG*RGARS LGARPPQVPGQHPTHGLGPQGPRSAWLLGSTAPR ACPGLGPTDF*AGPDIPQWMGRAPTFKSLQCVG TGPCEQQNCTVSFTSELDLFVG

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD RWSHVQPGSVSFPGRCLDRPACVLGHVWPPRTV GGRWLQRWRGQSWRQLLPQEMLQRIRTQGPGRC LSWAATG*AWTCTPGAASSCCTCVSSSLCSCCRW NSCV*ALTRTLSCWRPPWPSCLRACPASAPWSS KVSSTCSGPCLHLSLCPASAACSQRQTPVPAPA PGRPSRLPRCELFCSWSCPAPSPSSVITTPSVGL AGPWLLDPDAAQWGSLQTTVQMLFLGKVVGDGC QLR*AGGLHPCLLPFAGTSPVFLSVCPCLAGVG VGWEVCASRAAVSLAGRVDLGPRTLALRATSASP LPRRATPGHTGCLSPGCPDQPARWSEWPGPSGP PGPELQQPGDTAGLCPADARSGCALAVSQLPL* AA*GSGGPPRPHLPHSDTQPPADAAPSTGGPIHP AAPRSLSESAGHATS*DWRPGQPPGAQPGLQPA AEPPSLSG*VAPAPRHTGPTGGSLKPACLLQGP LHPQAWSTAASWLSLHLPGL*DSLSGFVRVL*AQ LWRRV*NSCSGRRQQECPSAVGWAGAKPKPATA RSGTSVLAAPCPAQQPPGLCAS*LGPPSTPHPA RPEGQPAPGPAP*AARRPLCAPAGEPPG*GLARR PEFTSGSPHSRNAQTVPDLRFGQLSCDPSRLLS DPGLWRPPAVPSGSHRHPHHHPLSAAAAGARPR PPGSS*RPAQPCAGAAAPWGGLPAGCGAVAALHP TAGPALP*SGGQDPE*QQLG*PGDLPGGRGTPA ALGSLPGAPLLLVPCGFPPCVQCLPGATGGDTA VLLGSSWGQSHLPPWGH*GASSSLHAGGAHGWPR AAGPPGRTRGCSEPPAVPVTERSPQLPPTGHRA AASALWHHRPQSGPLPPAPVVLGPSCSHLG*HH SSGGPGAHPPVRTLPGHTLLLVSAPQLSQPPSPS LYSPPHPQLSQVLALVHHQELCGRPGSEGLGAA AAAPCEPHRSAAAPGP*AGPAAVPAPKQGGRHP SAAAGAVPGPRAL*HGGDVRGRRVLCGLRARHRR GC*PP*LCGGQNLLCLLLAPEECEGGIRDHHSG PGGSGCAGPGVLLPWRGACAGARGG*GCPAEEG RRRPVDGHSAHQAAGGTEGQQRGGAGPRPRGDQG DMVELGVEPRA*CTFSFQQRLRGSEGPRRGAGL SLAPLNLGDAETGFLTQSNLLSVAGRLGLDWPA VALHLGVSYREVQRIRHEFRDDLDEQIRHMLFSW AERQAGQPGAVGLLVQALEQSDRQDVAEEVRAV LELGRRKYQDSIRRMGLAPKDPALPGSSAPQPP EPAQA*APQTFRLAQIFPSGWAEPPPSSLSSVWG RVPVSNKTALFLSPQSLIYLLD