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Sequences

Sequence LRDD.bAug05

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA SEPVLPPLPGPQRRATPGHTGCLSPGCPDQPARW SEWPGPSGPPGPELQQPGDTAGLCPADARSGCA LAVSQLPL*AA*GSGGPPRPHLPHSDTQPPADA APSTGGPIHPAAPRSLSESAGHATS*DWRPGQPP GAQPGLQPAAEPPSLSGGTSVLAAPCPAQQPPG LCAS*LGPPSTPHPARPEGQPAPGPAP*AARRP LCAPAGEPPG*GLARRPEFTSGSPHSRNAQTVPD LRFGQLSCDPSRLLSDPGLWRPPAVPSGSHRHP HHHPLSAAAAGARPRPPGSS*RPAQPCAGAAAP WGGLPAGCGAVAALHPTAGPALP*SGGQDPE*QQ LG*PGDLPGGRGTPAALGSLPGAPLLLVPCGFP PCVQCLPGATGGDTAVLLGSSWGQSHLPPWGH* GASSSLHAGGAHGWPRAAGPPGRTRGCSEPPAVP VTERSPQLPPTGHRAAASALWHHRPQSGPLPPA PVVLGPSCSHLG*HHSSGGPGAHPPVRTLPGHT LLLVLALVHHQELCGRPGSEGLGAAAAAPCEPHR SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC GGQNLLCLLLAPEECEGGIRDHHSGPGGSGCAGP GVLLPWRGACAGARGG*GCPAEEGRRRPVDGHS AHQAAETSRVRGATAGGWPLLGTLESGRCRDRL SDAEQPAECGWASGSGLASRGPAPGGVLPGGAAH PARVPG*SG*ADPSHALLLG*APGWAARGCGAP GAGPGAE*PAGRG*RGARSLGARPPQVPGQHPT HGLGPQGPRSAWLLGSTAPRACPGLGPTDF*AGP DIPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFT SELDLFVG

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP QSLSCLRSLVLKGGQRRDTLGACLRGALTNLPA GLSGLAHLAHLDLSFNSLETLPACVLQMRGLGAL LLSHNCLSELPEALGALPALTFLTVTHNRLQTL PPALGALSTLQRLDLSQNLLDTLPPEIGGLGSL LELNLASNRLQSLPASLAGLRSLRLLVLHSNLLA SVPADLARLPLLTRLDLRDNQLRDLPPELLDAP FVRLQGNPLGEASPDAPSSPVAALIPEMPRLFL TSDLDSFPVTPQGCSVTLACGVRLQFPAGATATP ITIRYRLLLPEPGLVPLGPHDALLSHVLELQPH GVAFQQDVGLWLLFTPPQARRCREVVVRTRNDN SWGDLETYLEEEAPQRLWAHCQVPHFSWFLVVSR PVSNACLVPPEGTLLCSSGHPGVKVIFPPGATE EPRRVSMQVVRMAGRELQALLGEPEAAVSPLLC LSQSGPPSFLQPVTVQLPLPSGITGLSLDRSRLH LLYWAPPAATWDDITAQVVLELTHLYARFQVTH FSWYWLWYTTKNCVGGLARKAWERLRLHRVNLI ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV EGRICFVFYSHLKNVKEVYVTTTLDREAQAVRG QVSFYRGAVPVRVPEEAEAARQRKGADALWMATL PIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGF LTQSNLLSVAGRLGLDWPAVALHLGVSYREVQR IRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLL VQALEQSDRQDVAEEVRAVLELGRRKYQDSIRR MGLAPKDPALPGSSAPQPPEPAQA*APQTFRLA QIFPSGWAEPPPSSLSSVWGRVPVSNKTALFLSP QSLIYLLD

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL RACPASAPWSSKEGNAGTHWVPVSGVP*PTCPL V*VAWPIWPTWT*ASTAWRHCRPVSCRCEVWVR SCCLTTASLSCLRLWGPSPPSPSSQ*HTTACRRC PQHWGPYPPCSASISLRICWTRYLLRLEAWAAS WSSTWPPTGCRASQPLWRDFGPCGSLSCTATSW PLCQLTWPAFHSSPGST*GTTSSGTCPLSC*TPP LCACRGTPWVRPRQTPRVHQWQPSFQKCPDCS* PQIWTAFL*PLKAAQ*PWPVASACSSQREPPPP PSPSAIGCCCRSQASSPWVLMTPCSAMCWSCSPM GWPSSRMWGCGCSSPHRRPGAAVKWWSGPGMTT AGVTWRPTWRKRHPSGSGLTARCPTSPGSLWFP ALCPMPAWCHRRGHCCAPRVILGSKSSSPLGPLR SLVESPCRWCAWLAESCRPSWENQRLQ*APCCA CHRAVPPASSNRSPCSCLCPLASQASVWTAPAC TCCTGPLLQPPGMTSQLRWSWSSPTCTHASRSHT SPGTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW RAESALSSTRT*RM*RRYT*PPLWTGRLRLCGA RCPSTVARCLCGCPRRLRLPGRGRAQTPCGWPL CPSSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF *RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSA SGTSSGMIWMSRSVTCSSPGLSARLGSQGLWGS WCRPWSRVTGRTWLKRCAQSWSSAAASTRTASDA WAWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWP RYSPVDGQSPHLQVSPVCGDGSL*ATKLHCFFH LRA*FICWM

Sequence LRDD.aAug05

GCCTGCGCCGCGGGCCTACCCGGAACGGCGCGTGGGCCCGGGCAGAAGCTGCTGGCGGTAGGCTCGCCTTGACTCCTTGGTGGTTCCCTGGCCGCGGCGA CCGGTGGTCCCACGTCCAGCCGGGCTCAGTGAGACGGAGTCTTGCTCTGTCTCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTACAACCTCCGC CTCCCGGGTTCACGCCATTCTTCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCGCATCACCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGA CGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTCAGCCACC GCGCCCGCTCAATATTTTATCTCTTAACGCCTCCTTCTCCCCTGCGCGATTGAAGGAGACAGTGCCAGGAGGCTCACTGCTTCTGGCCCAGAGTGATGGC TCGAAAGGAAGGGTCTTTGCAGGCCCAGAGAGGAGGTCCTGGCAGGCTTCCCGGAGGAGGCCGTTTGGGCTGGTTTGGAAAGGACACTGCAATTGTTCCG GCTTGTAGCAGGGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACG GTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGC TGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCAC TCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGAGGGCAACGCCGGGAC ACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGG AGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCC CGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAAT CTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGCGG GACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAG GGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGT TCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGG CCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGG TCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAG GCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCGGCTCTGGG CTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTC GGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAG GCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTC TGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGT GGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCT CGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAA ACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGC CTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTG TCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCTGCCCA TCAAGCTGCCGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGAC GCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGG CACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGC AGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGCATGGG CTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCCAGATA TTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACCTCAGA GCTTGATTTATTTGTTGGATG

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA TGGPTSSRAQ*DGVLLCLPGWSAVAPSRLTTTSA SRVHAILLPQPPE*LGLQARITTPG*FLYF**R RGFTMLARLVSNS*PRDPPASASQSAGITGVSH RARSIFYLLTPPSPLRD*RRQCQEAHCFWPRVMA RKEGSLQAQRGGPGRLPGGGRLGWFGKDTAIVP ACSRASPGAAWTGLPACWDMSGLQGPSVGDGCN GGGARAGGSCCRRRCFRGFGRRVQGAAFPGRQPA ELGPVPRGLPAAAAPVCPAASAAAAGGILASEH SRGPSAAGGHPGPAASEPVLPPLPGPQRRATPG HTGCLSPGCPDQPARWSEWPGPSGPPGPELQQPG DTAGLCPADARSGCALAVSQLPL*AA*GSGGPP RPHLPHSDTQPPADAAPSTGGPIHPAAPRSLSE SAGHATS*DWRPGQPPGAQPGLQPAAEPPSLSGG TSVLAAPCPAQQPPGLCAS*LGPPSTPHPARPE GQPAPGPAP*AARRPLCAPAGEPPG*GLARRPE FTSGSPHSRNAQTVPDLRFGQLSCDPSRLLSDPG LWRPPAVPSGSHRHPHHHPLSAAAAGARPRPPG SS*RPAQPCAGAAAPWGGLPAGCGAVAALHPTA GPALP*SGGQDPE*QQLG*PGDLPGGRGTPAALG SLPGAPLLLVPCGFPPCVQCLPGATGGDTAVLL GSSWGQSHLPPWGH*GASSSLHAGGAHGWPRAA GPPGRTRGCSEPPAVPVTERSPQLPPTGHRAAAS ALWHHRPQSGPLPPAPVVLGPSCSHLG*HHSSG GPGAHPPVRTLPGHTLLLVLALVHHQELCGRPG SEGLGAAAAAPCEPHRSAAAPGP*AGPAAVPAPK QGGRHPSAAAGAVPGPRAL*HGGDVRGRRVLCG LRARHRRGC*PP*LCGGQNLLCLLLAPEECEGG VLLPWRGACAGARGG*GCPAEEGRRRPVDGHSAH QAAETSRVRGATAGGWPLLGTLESGRCRDRLSD AEQPAECGWASGSGLASRGPAPGGVLPGGAAHP ARVPG*SG*ADPSHALLLG*APGWAARGCGAPGA GPGAE*PAGRG*RGARSLGARPPQVPGQHPTHG LGPQGPRSAWLLGSTAPRACPGLGPTDF*AGPD IPQWMGRAPTFKSLQCVGTGPCEQQNCTVSFTSE LDLFVG

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR PVVPRPAGLSETESCSVSQAGVQWHHLGSLQPP PPGFTPFFCLSLLSSWDYRRASPRPANFCIFSRD GVSPCWPGWSQTPDLVIHPPRPPKVLGLQASAT APAQYFIS*RLLLPCAIEGDSARRLTASGPE*W LERKGLCRPREEVLAGFPEEAVWAGLERTLQLFR LVAGLPRALPGQACLRAGTCLASKDRRWAMAAT VEGPELEAAAAAGDASEDSDAGSRALPFLGGNR LSLDLYPGGCQQLLHLCVQQPLQLLQVEFLRLST HEDPQLLEATLAQLPQSLSCLRSLVLKGGQRRD TLGACLRGALTNLPAGLSGLAHLAHLDLSFNSL ETLPACVLQMRGLGALLLSHNCLSELPEALGALP ALTFLTVTHNRLQTLPPALGALSTLQRLDLSQN LLDTLPPEIGGLGSLLELNLASNRLQSLPASLA GLRSLRLLVLHSNLLASVPADLARLPLLTRLDLR DNQLRDLPPELLDAPFVRLQGNPLGEASPDAPS SPVAALIPEMPRLFLTSDLDSFPVTPQGCSVTL ACGVRLQFPAGATATPITIRYRLLLPEPGLVPLG PHDALLSHVLELQPHGVAFQQDVGLWLLFTPPQ ARRCREVVVRTRNDNSWGDLETYLEEEAPQRLW AHCQVPHFSWFLVVSRPVSNACLVPPEGTLLCSS GHPGVKVIFPPGATEEPRRVSMQVVRMAGRELQ ALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQLP LPSGITGLSLDRSRLHLLYWAPPAATWDDITAQV VLELTHLYARFQVTHFSWYWLWYTTKNCVGGLA RKAWERLRLHRVNLIALQRRRDPEQVLLQCLPR NKVDATLRRLLERYRGPEPSDTVEMFEGEEFFAA FERGIDVDADRPDCVEGRICFVFYSHLKNVKEV SFYRGAVPVRVPEEAEAARQRKGADALWMATLP IKLPRLRGSEGPRRGAGLSLAPLNLGDAETGFLT QSNLLSVAGRLGLDWPAVALHLGVSYREVQRIR HEFRDDLDEQIRHMLFSWAERQAGQPGAVGLLV QALEQSDRQDVAEEVRAVLELGRRKYQDSIRRMG LAPKDPALPGSSAPQPPEPAQA*APQTFRLAQI FPSGWAEPPPSSLSSVWGRVPVSNKTALFLSPQ SLIYLLD

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD RWSHVQPGSVRRSLALSPRLECSGTISAHYNLR LPGSRHSSASAS*VAGTTGAHHHARLIFVFLVE TGFHHVGQAGLKLLTS*STRLGLPKCWDYRRQPP RPLNILSLNASFSPARLKETVPGGSLLLAQSDG SKGRVFAGPERRSWQASRRRPFGLVWKGHCNCS GL*QGFPGRCLDRPACVLGHVWPPRTVGGRWLQR WRGQSWRQLLPQEMLQRIRTQGPGRCLSWAATG *AWTCTPGAASSCCTCVSSSLCSCCRWNSCV*A LTRTLSCWRPPWPSCLRACPASAPWSSKEGNAGT HWVPVSGVP*PTCPLV*VAWPIWPTWT*ASTAW RHCRPVSCRCEVWVRSCCLTTASLSCLRLWGPS PPSPSSQ*HTTACRRCPQHWGPYPPCSASISLRI CWTRYLLRLEAWAASWSSTWPPTGCRASQPLWR DFGPCGSLSCTATSWPLCQLTWPAFHSSPGST* GTTSSGTCPLSC*TPPLCACRGTPWVRPRQTPRV HQWQPSFQKCPDCS*PQIWTAFL*PLKAAQ*PW PVASACSSQREPPPPPSPSAIGCCCRSQASSPW VLMTPCSAMCWSCSPMGWPSSRMWGCGCSSPHRR PGAAVKWWSGPGMTTAGVTWRPTWRKRHPSGSG LTARCPTSPGSLWFPALCPMPAWCHRRGHCCAP RVILGSKSSSPLGPLRSLVESPCRWCAWLAESCR PSWENQRLQ*APCCACHRAVPPASSNRSPCSCL CPLASQASVWTAPACTCCTGPLLQPPGMTSQLR WSWSSPTCTHASRSHTSPGTGSGTPPRTVWEAWL GRPGSGCGCTV*TSSLCSGAGTLSRSCCSACPE TRWTPPFGGCWSGTGAPSPLTRWRCSRAKSSLR PSSAASTWMLTALTVWRAESALSSTRT*RM*RRC PSTVARCLCGCPRRLRLPGRGRAQTPCGWPLCP SSCRDFEGPRGHGGGLASPWHP*IWEMPRPAF* RRATC*VWLGVWVWTGQPWPCTWGCPTGRCSASG TSSGMIWMSRSVTCSSPGLSARLGSQGLWGSWC RPWSRVTGRTWLKRCAQSWSSAAASTRTASDAW AWPPRTPLCLAPRLHSPQSLPRPRPHRLLGWPRY SPVDGQSPHLQVSPVCGDGSL*ATKLHCFFHLR A*FICWM

Sequence LRDD.gAug05

GCCGGCGGGATCCCAGGCGCCGAGCGCCCGCTGAGCAGCCACCCTTTGCGCGCCGCCTGCAGCGCAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTG CGTGCTGGGACATGTCTGGCCTCCAAGGACCGTCGGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTT CAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCCTTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCT GTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTGGAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCT CAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCAAAGGTGAGTTCCACCTGTTCTGGGCCCTGTCTCCATCTCTCGCTCTGCCCTGCCTCTGCTGCATGT TCTCAAAGGCAAACCCCAGTCCCAGCCCCAGCTCCAGGCCGCCCCTCCCGGCTCCCCAGGTGTGAACTGTTCTGCTCTTGGTCCTGTCCAGCCCCATCGC CATCCTCGGTGATCACCACCCCCAGTGTGGGGCTGGCTGGGCCCTGGCTCCTGGACCCTGACGCTGCCCAGTGGGGGTCTCTCCAGACCACAGTGCAGAT GTTGTTCCTGGGGAAGGTTGTGGGTGATGGTTGTCAGCTGAGATGAGCCGGTGGTCTCCACCCCTGCCTCCTTCCATTTGCGGGCACCTCTCCAGTGTTT CTGTCTGTCTGTCCCTGTCTGGCTGGGGTGGGGGTGGGGTGGGAGGTCTGTGCCTCTCGAGCAGCTGTTTCTCTGGCTGGCAGGGTCGATCTGGGACCTC GGACCCTGGCTCTGAGGGCCACATCCGCCTCCCCCCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGC CCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCACCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGG TGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAGCTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAG ACGCTGCCCCCAGCACTGGGGGCCCTATCCACCCTGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCA GCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCTGCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCGCCCCGACACACTGGCCCCACGGGAGGG TCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTCTGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGTCCCTCCACCTCCCTGGCCTTTGAGACT CCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCAGCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCGGCAGCAGGAGTGTCCCAGCGCCGTGGG CTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCCCGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGC TGACTTGGCCCGCCTTCCACTCCTCACCCGGCTCGACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTG CAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACGCCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGG ACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGTGACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCG CTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTCCCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTC CAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCCCACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACC TGGAGACCTACCTGGAGGAAGAGGCACCCCAGGTGAGGGCCACCCAGGCCTGCCGGTGGCGAGTGGAGAGCTGCTGCCCTGAGCCGTGCACCTCTGCCCA GAGCCTCACCCTGGCACCTTCCACCCTGCCCCGTCCCTCCTGGATCCTGCTTCCCTATGTCCCTGGCACCTTCCACCCCACCCCGTCCCTCCTGGATCCT GCTTCCCTGTGTCCCTGGCAACTTCCACCCCACCCCGTCCCTCCTGGATCCTGCTCCCTGTGTCCCTGGCACCTTCCACCCCGCCCCGTCTCTCCTGGAT CCTGCTCCCTGTGTCCCTGACTGGCTGTGCCCTGACCCAGGCTCCTGTGACCTCCTCTCTCCCCCATCCCAGCGGCTCTGGGCTCACTGCCAGGTGCCCC ACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTGTGCTCCTCGGGTCATCCTGGGGTCAA AGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAGAGCTGCAGGCCCTCCTGGGAGAACCA GAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCAGCTGCCTCTGCCCTCTGGCATCACAG GCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACAGCTCAGGTGGTCCTGGAGCTCACCCA CCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTCAGTGCCCCCCAGCTTTCTCAGCCCCCCTCCCCCAGTCTGTACAGCCCTCCTCACCCCCAGC TCTCCCAGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCTGCGGCTGCACCGTGTGAACCTCATCG CTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTTCGGCGGCTGCTGGAGCGGTACCGGGG CCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACGTGGATGCTGACCGCCCTGACTGTGTG GAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTG AGGCTGCCCGGCAGAGGAAGGGCGCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGGTGGGACTGAGGGACAGCAGAGGGGCGGGGCAGGA CCGAGGCCCAGGGGTGACCAGGGTGACATGGTGGAGTTGGGGGTGGAGCCCAGGGCTTAATGCACTTTTTCCTTCCAACAGAGACTTCGAGGGTCCGAGG GGCCACGGCGGGGGGCTGGCCTCTCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCG TCTGGGTCTGGACTGGCCAGCCGTGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGTGAGGCTCCCACTGCCCG CTGGCTGCCAGCCCCAGGGCTGAGCCCTGGCTGCCTGGAGGGCCCACCTGATTCTGACAGAAGTTTCTTCCAGGGGAGGTGGGGGAAGGGCAAACACCAG GCTGGAAAGGAAAGGTCTGGCCGCACCTGGCCCCAGGAGCCGGGAACTCCCCCACACTCCGAGGCAGAGTGTGGTGGGGGGTCCTGGGCAGGTGGACAGG ACCCCTCTGTCGCCCATCCTCTGCCTGCAGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTGGGCTGAGCGCCAGGCTGGGCAGCCAGGG GCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTCTTGGAGCTCGGCCGCCGCAAGTACCAGG ACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAGAGCCTGCCCAGGCCTAGGCCCCACAGAC TTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGGACGGGTCCCTGTGAGCAACAAAACTGCA CTGTTTCTTTC

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

AGGIPGAERPLSSHPLRAACSAASPGAAWTGLP ACWDMSGLQGPSVGDGCNGGGARAGGSCCRRRCF RGFGRRVQGAAFPGRQPAELGPVPRGLPAAAAP VCPAASAAAAGGILASEHSRGPSAAGGHPGPAA SEPVLPPLPGPQR*VPPVLGPVSISRSALPLLHV LKGKPQSQPQLQAAPPGSPGVNCSALGPVQPHR HPR*SPPPVWGWLGPGSWTLTLPSGGLSRPQCR CCSWGRLWVMVVS*DEPVVSTPASFHLRAPLQCF CLSVPVWLGWGWGGRSVPLEQLFLWLAGSIWDL GPWL*GPHPPPPFPGGQRRDTLGACLRGALTNL PAGLSGLAHLAHLDLSFNSLETLPACVLQMRGLG ALLLSHNCLSELPEALGALPALTFLTVTHNRLQ TLPPALGALSTLQRLDLSQNLLDTLPPEIGGLG SLLELNLASNRLQSLPASLGE*PLRPDTLAPREG P*SLPVFCRGLCTHRLGPQLPLGCPSTSLAFET PSVALSEFSEPSCGGESETAALGGGSRSVPAPW AGLVPSLSQQLPAAGLRSLRLLVLHSNLLASVPA DLARLPLLTRLDLRDNQLRDLPPELLDAPFVRL QGNPLGEASPDAPSSPVAALIPEMPRLFLTSDL DSFPVTPQGCSVTLACGVRLQFPAGATATPITIR YRLLLPEPGLVPLGPHDALLSHVLELQPHGVAF QQDVGLWLLFTPPQARRCREVVVRTRNDNSWGD LETYLEEEAPQVRATQACRWRVESCCPEPCTSAQ SLTLAPSTLPRPSWILLPYVPGTFHPTPSLLDP ASLCPWQLPPHPVPPGSCSLCPWHLPPRPVSPG SCSLCP*LAVP*PRLL*PPLSPIPAALGSLPGAP LLLVPCGFPPCVQCLPGATGGDTAVLLGSSWGQ SHLPPWGH*GASSSLHAGGAHGWPRAAGPPGRT RGCSEPPAVPVTERSPQLPPTGHRAAASALWHHR PQSGPLPPAPVVLGPSCSHLG*HHSSGGPGAHP PVRTLPGHTLLLVSAPQLSQPPSPSLYSPPHPQ LSQVLALVHHQELCGRPGSEGLGAAAAAPCEPHR SAAAPGP*AGPAAVPAPKQGGRHPSAAAGAVPG PRAL*HGGDVRGRRVLCGLRARHRRGC*PP*LC GGQNLLCLLLAPEECEGGVLLPWRGACAGARGG* GCPAEEGRRRPVDGHSAHQAAGGTEGQQRGGAG PRPRGDQGDMVELGVEPRA*CTFSFQQRLRGSE GPRRGAGLSLAPLNLGDAETGFLTQSNLLSVAGR LGLDWPAVALHLGVSYREVQRIRHEFR*GSHCP LAASPRAEPWLPGGPT*F*QKFLPGEVGEGQTP GWKGKVWPHLAPGAGNSPTLRGRVWWGVLGRWTG PLCRPSSACRDDLDEQIRHMLFSWAERQAGQPG AVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQ DSIRRMGLAPKDPALPGSSAPQPPEPAQA*APQT FRLAQIFPSGWAEPPPSSLSSVWGRVPVSNKTA LFL

Frame 1

PAGSQAPSAR*AATLCAPPAAQLPRALPGQACL RAGTCLASKDRRWAMAATVEGPELEAAAAAGDA SEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHL CVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLP QSLSCLRSLVLKGEFHLFWALSPSLALPCLCCM FSKANPSPSPSSRPPLPAPQV*TVLLLVLSSPIA ILGDHHPQCGAGWALAPGP*RCPVGVSPDHSAD VVPGEGCG*WLSAEMSRWSPPLPPSICGHLSSV SVCLSLSGWGGGGVGGLCLSSSCFSGWQGRSGTS DPGSEGHIRLPPSQEGNAGTHWVPVSGVP*PTC PLV*VAWPIWPTWT*ASTAWRHCRPVSCRCEVW VRSCCLTTASLSCLRLWGPSPPSPSSQ*HTTACR RCPQHWGPYPPCSASISLRICWTRYLLRLEAWA ASWSSTWPPTGCRASQPLWVSSPCAPTHWPHGR VPEACLSSAGASAPTGLVHSCLLVVPPPPWPLRL PQWLCQSSLSPAVEESLKQLLWEAAAGVSQRRG LGWCQA*ASNCPQRDFGPCGSLSCTATSWPLCQ LTWPAFHSSPGST*GTTSSGTCPLSC*TPPLCAC RGTPWVRPRQTPRVHQWQPSFQKCPDCS*PQIW TAFL*PLKAAQ*PWPVASACSSQREPPPPPSPS AIGCCCRSQASSPWVLMTPCSAMCWSCSPMGWPS SRMWGCGCSSPHRRPGAAVKWWSGPGMTTAGVT WRPTWRKRHPR*GPPRPAGGEWRAAALSRAPLP RASPWHLPPCPVPPGSCFPMSLAPSTPPRPSWIL LPCVPGNFHPTPSLLDPAPCVPGTFHPAPSLLD PAPCVPDWLCPDPGSCDLLSPPSQRLWAHCQVP HFSWFLVVSRPVSNACLVPPEGTLLCSSGHPGVK VIFPPGATEEPRRVSMQVVRMAGRELQALLGEP EAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGIT GLSLDRSRLHLLYWAPPAATWDDITAQVVLELTH LYARFQVTHFSWSVPPSFLSPPPPVCTALLTPS SPRYWLWYTTKNCVGGLARKAWERLRLHRVNLI ALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRG PEPSDTVEMFEGEEFFAAFERGIDVDADRPDCV EGRICFVFYSHLKNVKEVSFYRGAVPVRVPEEA EAARQRKGADALWMATLPIKLPVGLRDSRGAGQD RGPGVTRVTWWSWGWSPGLNALFPSNRDFEGPR GHGGGLASPWHP*IWEMPRPAF*RRATC*VWLG VWVWTGQPWPCTWGCPTGRCSASGTSSGEAPTAR WLPAPGLSPGCLEGPPDSDRSFFQGRWGKGKHQ AGKERSGRTWPQEPGTPPHSEAECGGGSWAGGQ DPSVAHPLPAGMIWMSRSVTCSSPGLSARLGSQG LWGSWCRPWSRVTGRTWLKRCAQSWSSAAASTR TASDAWAWPPRTPLCLAPRLHSPQSLPRPRPHR LLGWPRYSPVDGQSPHLQVSPVCGDGSL*ATKLH CFF

Frame 2

RRDPRRRAPAEQPPFARRLQRSFPGRCLDRPAC VLGHVWPPRTVGGRWLQRWRGQSWRQLLPQEML QRIRTQGPGRCLSWAATG*AWTCTPGAASSCCT CVSSSLCSCCRWNSCV*ALTRTLSCWRPPWPSCL RACPASAPWSSKVSSTCSGPCLHLSLCPASAAC SQRQTPVPAPAPGRPSRLPRCELFCSWSCPAPS PSSVITTPSVGLAGPWLLDPDAAQWGSLQTTVQM LFLGKVVGDGCQLR*AGGLHPCLLPFAGTSPVF LSVCPCLAGVGVGWEVCASRAAVSLAGRVDLGP RTLALRATSASPLPRRATPGHTGCLSPGCPDQPA RWSEWPGPSGPPGPELQQPGDTAGLCPADARSG CALAVSQLPL*AA*GSGGPPRPHLPHSDTQPPA DAAPSTGGPIHPAAPRSLSESAGHATS*DWRPGQ PPGAQPGLQPAAEPPSLSG*VAPAPRHTGPTGG SLKPACLLQGPLHPQAWSTAASWLSLHLPGL*D SLSGFVRVL*AQLWRRV*NSCSGRRQQECPSAVG WAGAKPKPATARSGTSVLAAPCPAQQPPGLCAS *LGPPSTPHPARPEGQPAPGPAP*AARRPLCAP AGEPPG*GLARRPEFTSGSPHSRNAQTVPDLRFG QLSCDPSRLLSDPGLWRPPAVPSGSHRHPHHHP LSAAAAGARPRPPGSS*RPAQPCAGAAAPWGGL PAGCGAVAALHPTAGPALP*SGGQDPE*QQLG*P GDLPGGRGTPGEGHPGLPVASGELLP*AVHLCP EPHPGTFHPAPSLLDPASLCPWHLPPHPVPPGS CFPVSLATSTPPRPSWILLPVSLAPSTPPRLSWI LLPVSLTGCALTQAPVTSSLPHPSGSGLTARCP TSPGSLWFPALCPMPAWCHRRGHCCAPRVILGS KSSSPLGPLRSLVESPCRWCAWLAESCRPSWENQ RLQ*APCCACHRAVPPASSNRSPCSCLCPLASQ ASVWTAPACTCCTGPLLQPPGMTSQLRWSWSSP TCTHASRSHTSPGQCPPAFSAPLPQSVQPSSPPA LPGTGSGTPPRTVWEAWLGRPGSGCGCTV*TSS LCSGAGTLSRSCCSACPETRWTPPFGGCWSGTG APSPLTRWRCSRAKSSLRPSSAASTWMLTALTVW RAESALSSTRT*RM*RRCPSTVARCLCGCPRRL RLPGRGRAQTPCGWPLCPSSCRWD*GTAEGRGR TEAQG*PG*HGGVGGGAQGLMHFFLPTETSRVRG ATAGGWPLLGTLESGRCRDRLSDAEQPAECGWA SGSGLASRGPAPGGVLPGGAAHPARVPVRLPLP AGCQPQG*ALAAWRAHLILTEVSSRGGGGRANTR LERKGLAAPGPRSRELPHTPRQSVVGGPGQVDR TPLSPILCLQG*SG*ADPSHALLLG*APGWAAR GCGAPGAGPGAE*PAGRG*RGARSLGARPPQVPG QHPTHGLGPQGPRSAWLLGSTAPRACPGLGPTD F*AGPDIPQWMGRAPTFKSLQCVGTGPCEQQNC TVSF

Sequence LRDD.fAug05

GCCTGCGCCGCGGGCCTACCCGGAACGGCGCGTGGGCCCGGGCAGAAGCTGCTGGCGGTAGGCTCGCCTTGACTCCTTGGTGGTTCCCTGGCCGCGGCGA CCGGTGGTCCCACGTCCAGCCGGGCTCAGTGAGCTTCCCCGGGCGCTGCCTGGACAGGCCTGCCTGCGTGCTGGGACATGTCTGGCCTCCAAGGACCGTC GGTGGGCGATGGCTGCAACGGTGGAGGGGCCAGAGCTGGAGGCAGCTGCTGCCGCAGGAGATGCTTCAGAGGATTCGGACGCAGGGTCCAGGGCGCTGCC TTTCCTGGGCGGCAACCGGCTGAGCTTGGACCTGTACCCCGGGGGCTGCCAGCAGCTGCTGCACCTGTGTGTCCAGCAGCCTCTGCAGCTGCTGCAGGTG GAATTCTTGCGTCTGAGCACTCACGAGGACCCTCAGCTGCTGGAGGCCACCCTGGCCCAGCTGCCTCAGAGCCTGTCCTGCCTCCGCTCCCTGGTCCTCA AAGGTGAGTTCCACCTGTTCTGGGCCCTGTCTCCATCTCTCGCTCTGCCCTGCCTCTGCTGCATGTTCTCAAAGGCAAACCCCAGTCCCAGCCCCAGCTC CAGGCCGCCCCTCCCGGCTCCCCAGGTGTGAACTGTTCTGCTCTTGGTCCTGTCCAGCCCCATCGCCATCCTCGGTGATCACCACCCCCAGTGTGGGGCT GGCTGGGCCCTGGCTCCTGGACCCTGACGCTGCCCAGTGGGGGTCTCTCCAGACCACAGTGCAGATGTTGTTCCTGGGGAAGGTTGTGGGTGATGGTTGT CAGCTGAGATGAGCCGGTGGTCTCCACCCCTGCCTCCTTCCATTTGCGGGCACCTCTCCAGTGTTTCTGTCTGTCTGTCCCTGTCTGGCTGGGGTGGGGG TGGGGTGGGAGGTCTGTGCCTCTCGAGCAGCTGTTTCTCTGGCTGGCAGGGTCGATCTGGGACCTCGGACCCTGGCTCTGAGGGCCACATCCGCCTCCCC CCTTCCCAGGAGGGCAACGCCGGGACACACTGGGTGCCTGTCTCCGGGGTGCCCTGACCAACCTGCCCGCTGGTCTGAGTGGCCTGGCCCATCTGGCCCA CCTGGACCTGAGCTTCAACAGCCTGGAGACACTGCCGGCCTGTGTCCTGCAGATGCGAGGTCTGGGTGCGCTCTTGCTGTCTCACAACTGCCTCTCTGAG CTGCCTGAGGCTCTGGGGGCCCTCCCCGCCCTCACCTTCCTCACAGTGACACACAACCGCCTGCAGACGCTGCCCCCAGCACTGGGGGCCCTATCCACCC TGCAGCGCCTCGATCTCTCTCAGAATCTGCTGGACACGCTACCTCCTGAGATTGGAGGCCTGGGCAGCCTCCTGGAGCTCAACCTGGCCTCCAACCGGCT GCAGAGCCTCCCAGCCTCTCTGGGTGAGTAGCCCCTGCGCCCCGACACACTGGCCCCACGGGAGGGTCCCTGAAGCCTGCCTGTCTTCTGCAGGGGCCTC TGCACCCACAGGCTTGGTCCACAGCTGCCTCTTGGTTGTCCCTCCACCTCCCTGGCCTTTGAGACTCCCTCAGTGGCTTTGTCAGAGTTCTCTGAGCCCA GCTGTGGAGGAGAGTCTGAAACAGCTGCTCTGGGAGGCGGCAGCAGGAGTGTCCCAGCGCCGTGGGCTGGGCTGGTGCCAAGCCTAAGCCAGCAACTGCC CGCAGCGGGACTTCGGTCCTTGCGGCTCCTTGTCCTGCACAGCAACCTCCTGGCCTCTGTGCCAGCTGACTTGGCCCGCCTTCCACTCCTCACCCGGCTC GACCTGAGGGACAACCAGCTCCGGGACCTGCCCCCTGAGCTGCTAGACGCCCCCTTTGTGCGCCTGCAGGGGAACCCCCTGGGTGAGGCCTCGCCAGACG CCCCGAGTTCACCAGTGGCAGCCCTCATTCCAGAAATGCCCAGACTGTTCCTGACCTCAGATTTGGACAGCTTTCCTGTGACCCCTCAAGGCTGCTCAGT GACCCTGGCCTGTGGCGTCCGCCTGCAGTTCCCAGCGGGAGCCACCGCCACCCCCATCACCATCCGCTATCGGCTGCTGCTGCCGGAGCCAGGCCTCGTC CCCCTGGGTCCTCATGACGCCCTGCTCAGCCATGTGCTGGAGCTGCAGCCCCATGGGGTGGCCTTCCAGCAGGATGTGGGGCTGTGGCTGCTCTTCACCC CACCGCAGGCCCGGCGCTGCCGTGAAGTGGTGGTCAGGACCCGGAATGACAACAGCTGGGGTGACCTGGAGACCTACCTGGAGGAAGAGGCACCCCAGCG GCTCTGGGCTCACTGCCAGGTGCCCCACTTCTCCTGGTTCCTTGTGGTTTCCCGCCCTGTGTCCAATGCCTGCCTGGTGCCACCGGAGGGGACACTGCTG TGCTCCTCGGGTCATCCTGGGGTCAAAGTCATCTTCCCCCCTGGGGCCACTGAGGAGCCTCGTCGAGTCTCCATGCAGGTGGTGCGCATGGCTGGCCGAG AGCTGCAGGCCCTCCTGGGAGAACCAGAGGCTGCAGTGAGCCCCCTGCTGTGCCTGTCACAGAGCGGTCCCCCCAGCTTCCTCCAACCGGTCACCGTGCA GCTGCCTCTGCCCTCTGGCATCACAGGCCTCAGTCTGGACCGCTCCCGCCTGCACCTGTTGTACTGGGCCCCTCCTGCAGCCACCTGGGATGACATCACA GCTCAGGTGGTCCTGGAGCTCACCCACCTGTACGCACGCTTCCAGGTCACACACTTCTCCTGGTCAGTGCCCCCCAGCTTTCTCAGCCCCCCTCCCCCAG TCTGTACAGCCCTCCTCACCCCCAGCTCTCCCAGGTACTGGCTCTGGTACACCACCAAGAACTGTGTGGGAGGCCTGGCTCGGAAGGCCTGGGAGCGGCT GCGGCTGCACCGTGTGAACCTCATCGCTCTGCAGCGGCGCCGGGACCCTGAGCAGGTCCTGCTGCAGTGCCTGCCCCGAAACAAGGTGGACGCCACCCTT CGGCGGCTGCTGGAGCGGTACCGGGGCCCCGAGCCCTCTGACACGGTGGAGATGTTCGAGGGCGAAGAGTTCTTTGCGGCCTTCGAGCGCGGCATCGACG TGGATGCTGACCGCCCTGACTGTGTGGAGGGCAGAATCTGCTTTGTCTTCTACTCGCACCTGAAGAATGTGAAGGAGGTATACGTGACCACCACTCTGGA CCGGGAGGCTCAGGCTGTGCGGGGCCAGGTGTCCTTCTACCGTGGCGCGGTGCCTGTGCGGGTGCCCGAGGAGGCTGAGGCTGCCCGGCAGAGGAAGGGC GCAGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGGTGGGACTGAGGGACAGCAGAGGGGCGGGGCAGGACCGAGGCCCAGGGGTGACCAGGG TGACATGGTGGAGTTGGGGGTGGAGCCCAGGGCTTAATGCACTTTTTCCTTCCAACAGAGACTTCGAGGGTCCGAGGGGCCACGGCGGGGGGCTGGCCTC TCCTTGGCACCCTTGAATCTGGGAGATGCCGAGACCGGCTTTCTGACGCAGAGCAACCTGCTGAGTGTGGCTGGGCGTCTGGGTCTGGACTGGCCAGCCG TGGCCCTGCACCTGGGGGTGTCCTACCGGGAGGTGCAGCGCATCCGGCACGAGTTCCGGGATGATCTGGATGAGCAGATCCGTCACATGCTCTTCTCCTG GGCTGAGCGCCAGGCTGGGCAGCCAGGGGCTGTGGGGCTCCTGGTGCAGGCCCTGGAGCAGAGTGACCGGCAGGACGTGGCTGAAGAGGTGCGCGCAGTC TTGGAGCTCGGCCGCCGCAAGTACCAGGACAGCATCCGACGCATGGGCTTGGCCCCCAAGGACCCCGCTCTGCCTGGCTCCTCGGCTCCACAGCCCCCAG AGCCTGCCCAGGCCTAGGCCCCACAGACTTTTAGGCTGGCCCAGATATTCCCCAGTGGATGGGCAGAGCCCCCACCTTCAAGTCTCTCCAGTGTGTGGGG ACGGGTCCCTGTGAGCAACAAAACTGCACTGTTTCTTTCACCTCAGAGCTTGATTTATTTGTTGGATG

LRDD.u.f.7 (forward primer)
LRDD.u.r.3 (reverse primer)
Intron retention

Frame 0

ACAAGLPGTARGPGQKLLAVGSP*LLGGSLAAA TGGPTSSRAQ*ASPGAAWTGLPACWDMSGLQGPS VGDGCNGGGARAGGSCCRRRCFRGFGRRVQGAA FPGRQPAELGPVPRGLPAAAAPVCPAASAAAAG GILASEHSRGPSAAGGHPGPAASEPVLPPLPGPQ R*VPPVLGPVSISRSALPLLHVLKGKPQSQPQL QAAPPGSPGVNCSALGPVQPHRHPR*SPPPVWG WLGPGSWTLTLPSGGLSRPQCRCCSWGRLWVMVV S*DEPVVSTPASFHLRAPLQCFCLSVPVWLGWG WGGRSVPLEQLFLWLAGSIWDLGPWL*GPHPPP PFPGGQRRDTLGACLRGALTNLPAGLSGLAHLAH LDLSFNSLETLPACVLQMRGLGALLLSHNCLSE LPEALGALPALTFLTVTHNRLQTLPPALGALST LQRLDLSQNLLDTLPPEIGGLGSLLELNLASNRL QSLPASLGE*PLRPDTLAPREGP*SLPVFCRGL CTHRLGPQLPLGCPSTSLAFETPSVALSEFSEP SCGGESETAALGGGSRSVPAPWAGLVPSLSQQLP AAGLRSLRLLVLHSNLLASVPADLARLPLLTRL DLRDNQLRDLPPELLDAPFVRLQGNPLGEASPD APSSPVAALIPEMPRLFLTSDLDSFPVTPQGCSV TLACGVRLQFPAGATATPITIRYRLLLPEPGLV PLGPHDALLSHVLELQPHGVAFQQDVGLWLLFT PPQARRCREVVVRTRNDNSWGDLETYLEEEAPQR LWAHCQVPHFSWFLVVSRPVSNACLVPPEGTLL CSSGHPGVKVIFPPGATEEPRRVSMQVVRMAGR ELQALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQ LPLPSGITGLSLDRSRLHLLYWAPPAATWDDIT AQVVLELTHLYARFQVTHFSWSVPPSFLSPPPP VCTALLTPSSPRYWLWYTTKNCVGGLARKAWERL RLHRVNLIALQRRRDPEQVLLQCLPRNKVDATL RRLLERYRGPEPSDTVEMFEGEEFFAAFERGID VDADRPDCVEGRICFVFYSHLKNVKEVYVTTTLD REAQAVRGQVSFYRGAVPVRVPEEAEAARQRKG ADALWMATLPIKLPVGLRDSRGAGQDRGPGVTR VTWWSWGWSPGLNALFPSNRDFEGPRGHGGGLAS PWHP*IWEMPRPAF*RRATC*VWLGVWVWTGQP WPCTWGCPTGRCSASGTSSGMIWMSRSVTCSSP GLSARLGSQGLWGSWCRPWSRVTGRTWLKRCAQS WSSAAASTRTASDAWAWPPRTPLCLAPRLHSPQ SLPRPRPHRLLGWPRYSPVDGQSPHLQVSPVCG DGSL*ATKLHCFFHLRA*FICWM

Frame 1

PAPRAYPERRVGPGRSCWR*ARLDSLVVPWPRR PVVPRPAGLSELPRALPGQACLRAGTCLASKDR RWAMAATVEGPELEAAAAAGDASEDSDAGSRALP FLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQV EFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVL KGEFHLFWALSPSLALPCLCCMFSKANPSPSPSS RPPLPAPQV*TVLLLVLSSPIAILGDHHPQCGA GWALAPGP*RCPVGVSPDHSADVVPGEGCG*WL SAEMSRWSPPLPPSICGHLSSVSVCLSLSGWGGG GVGGLCLSSSCFSGWQGRSGTSDPGSEGHIRLP PSQEGNAGTHWVPVSGVP*PTCPLV*VAWPIWP TWT*ASTAWRHCRPVSCRCEVWVRSCCLTTASLS CLRLWGPSPPSPSSQ*HTTACRRCPQHWGPYPP CSASISLRICWTRYLLRLEAWAASWSSTWPPTG CRASQPLWVSSPCAPTHWPHGRVPEACLSSAGAS APTGLVHSCLLVVPPPPWPLRLPQWLCQSSLSP AVEESLKQLLWEAAAGVSQRRGLGWCQA*ASNC PQRDFGPCGSLSCTATSWPLCQLTWPAFHSSPGS T*GTTSSGTCPLSC*TPPLCACRGTPWVRPRQT PRVHQWQPSFQKCPDCS*PQIWTAFL*PLKAAQ *PWPVASACSSQREPPPPPSPSAIGCCCRSQASS PWVLMTPCSAMCWSCSPMGWPSSRMWGCGCSSP HRRPGAAVKWWSGPGMTTAGVTWRPTWRKRHPS GSGLTARCPTSPGSLWFPALCPMPAWCHRRGHCC APRVILGSKSSSPLGPLRSLVESPCRWCAWLAE SCRPSWENQRLQ*APCCACHRAVPPASSNRSPC SCLCPLASQASVWTAPACTCCTGPLLQPPGMTSQ LRWSWSSPTCTHASRSHTSPGQCPPAFSAPLPQ SVQPSSPPALPGTGSGTPPRTVWEAWLGRPGSG CGCTV*TSSLCSGAGTLSRSCCSACPETRWTPPF GGCWSGTGAPSPLTRWRCSRAKSSLRPSSAAST WMLTALTVWRAESALSSTRT*RM*RRYT*PPLW TGRLRLCGARCPSTVARCLCGCPRRLRLPGRGRA QTPCGWPLCPSSCRWD*GTAEGRGRTEAQG*PG *HGGVGGGAQGLMHFFLPTETSRVRGATAGGWP LLGTLESGRCRDRLSDAEQPAECGWASGSGLASR GPAPGGVLPGGAAHPARVPG*SG*ADPSHALLL G*APGWAARGCGAPGAGPGAE*PAGRG*RGARS LGARPPQVPGQHPTHGLGPQGPRSAWLLGSTAPR ACPGLGPTDF*AGPDIPQWMGRAPTFKSLQCVG TGPCEQQNCTVSFTSELDLFVG

Frame 2

LRRGPTRNGAWARAEAAGGRLALTPWWFPGRGD RWSHVQPGSVSFPGRCLDRPACVLGHVWPPRTV GGRWLQRWRGQSWRQLLPQEMLQRIRTQGPGRC LSWAATG*AWTCTPGAASSCCTCVSSSLCSCCRW NSCV*ALTRTLSCWRPPWPSCLRACPASAPWSS KVSSTCSGPCLHLSLCPASAACSQRQTPVPAPA PGRPSRLPRCELFCSWSCPAPSPSSVITTPSVGL AGPWLLDPDAAQWGSLQTTVQMLFLGKVVGDGC QLR*AGGLHPCLLPFAGTSPVFLSVCPCLAGVG VGWEVCASRAAVSLAGRVDLGPRTLALRATSASP LPRRATPGHTGCLSPGCPDQPARWSEWPGPSGP PGPELQQPGDTAGLCPADARSGCALAVSQLPL* AA*GSGGPPRPHLPHSDTQPPADAAPSTGGPIHP AAPRSLSESAGHATS*DWRPGQPPGAQPGLQPA AEPPSLSG*VAPAPRHTGPTGGSLKPACLLQGP LHPQAWSTAASWLSLHLPGL*DSLSGFVRVL*AQ LWRRV*NSCSGRRQQECPSAVGWAGAKPKPATA RSGTSVLAAPCPAQQPPGLCAS*LGPPSTPHPA RPEGQPAPGPAP*AARRPLCAPAGEPPG*GLARR PEFTSGSPHSRNAQTVPDLRFGQLSCDPSRLLS DPGLWRPPAVPSGSHRHPHHHPLSAAAAGARPR PPGSS*RPAQPCAGAAAPWGGLPAGCGAVAALHP TAGPALP*SGGQDPE*QQLG*PGDLPGGRGTPA ALGSLPGAPLLLVPCGFPPCVQCLPGATGGDTA VLLGSSWGQSHLPPWGH*GASSSLHAGGAHGWPR AAGPPGRTRGCSEPPAVPVTERSPQLPPTGHRA AASALWHHRPQSGPLPPAPVVLGPSCSHLG*HH SSGGPGAHPPVRTLPGHTLLLVSAPQLSQPPSPS LYSPPHPQLSQVLALVHHQELCGRPGSEGLGAA AAAPCEPHRSAAAPGP*AGPAAVPAPKQGGRHP SAAAGAVPGPRAL*HGGDVRGRRVLCGLRARHRR GC*PP*LCGGQNLLCLLLAPEECEGGIRDHHSG PGGSGCAGPGVLLPWRGACAGARGG*GCPAEEG RRRPVDGHSAHQAAGGTEGQQRGGAGPRPRGDQG DMVELGVEPRA*CTFSFQQRLRGSEGPRRGAGL SLAPLNLGDAETGFLTQSNLLSVAGRLGLDWPA VALHLGVSYREVQRIRHEFRDDLDEQIRHMLFSW AERQAGQPGAVGLLVQALEQSDRQDVAEEVRAV LELGRRKYQDSIRRMGLAPKDPALPGSSAPQPP EPAQA*APQTFRLAQIFPSGWAEPPPSSLSSVWG RVPVSNKTALFLSPQSLIYLLD