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Sequences

Sequence FGFR1OP.bAug05

GTCGGCCGCGTAGCCCGCGCGCGGAGCGTACCCTGCTGCGGCCGTTGGCCGTTAGCGCGGCTTCGGCGGTTGTCTTGGAGAAGCAAGATGGCGGCGACGG
CGGCCGCAGTGGTGGCCGAGGAGGACACGGAGCTGCGGGACCTGCTGGTGCAGACGCTGGAGAACAGCGGGGTCCTGAACCGCATCAAGGCTGAACTCCG
AGCAGCTGTGTTTTTAGCACTAGAGGAGCAAGAAAAAGTAGAGAACAAAACTCCTTTAGTTAATGAGAGCCTGAAAAAGTTTTTAAATACCAAAGACGGT
CGTTTAGTGGCTAGTCTTGTTGCAGAATTTCTTCAGTTTTTTAACCTTGACTTTACTTTGGCTGTTTTTCAACCTGAAACTAGCACACTGCAAGGTCTCG
AAGGTCGAGAGAATTTAGCCCGAGATTTAGGTATAATTGAAGCAGAAGGTACTGTGGGTGGACCCTTATTATTAGAAGTGATCAGGCGCTGTCAACAGAA
AGAAAAAGGGCCAACCACTGGGGAAGGTGCACTTGATCTATCTGATGTACATTCTCCACCAAAGTCACCAGAGGGAAAAACAAGTGCACAGACAACACCA
AGTAAGAAGGCCAATGATGAGGCCAATCAGAGTGATACAAGTGTCTCCTTGTCAGAACCCAAGAGCAAAAGCAGCCTTCACTTACTGTCCCATGAAACAA
AAATTGGATCTTTTCTAAGCAACAGAACTTTAGATGGCAAAGACAAAGCTGGCCTTTGTCCAGATGAAGATGATATGGAAGGAGATTCTTTCTTTGATGA
TCCCATTCCTAAGCCAGAGAAAACTTACGGTTTGAGGAAGGAACCTAGGAAGCAAGCAGGAAGTCTGGCCTCGCTCTCGGATGCACCCCCCTTAAAAAGT
GGACTCAGCTCCCTGGCGGGAGCCCCTTCTTTAAAAGACTCTGAGAGTAAAAGGGGAAATACAGTTTTGAAAGATCTGAAATTGATCAGTGATAAAATTG
GATCACTTGGATTAGGAACTGGAGAAGATGATGACTATGTTGATGATTTTAATAGTACCAGCCATCGCTCAGAGAAAAGTGAGATAAGTATTGGTGAAGA
GATAGAAGAAGACCTTTCTGTGGAAATAGATGACATCAATACCAGTGATAAGCTTGATGACCTCACACAAGATCTGACTGTATCCCAGCTCAGTGATGTT
GCGGATTATCTGGAAGATGTTGCATAGACACGAAGAAGGAAGTATTCTAATTAACAAGGACAGAGGACTGACCGGTTCCATTTTTTTTTTTTCCAGACAA
TCACTCAGCTGGAATGTCTGCTCTCTATTGGTGCCTTGCATTTCAAAAACACTGCAGATATTTTTTAAAAGTAATTTTCATTTTACTAAACAAAATACTT
CCTATTTGAGCCCATGTGTGGAAGATTTAATATTCTTAATTTAACTGTACATTTCTTTATGGAAATTGATTATCTACACTCAGTTTCATTACAGGGAAGG
AACCCATGAAAACATCAGTGTTAAGAGCATGATGAAAGGTGTCAATAAAGCCGTAGGATCGCGCAACCCTTTGTGTGTGTGGCTGCTGGTACGTGTGATC
TTTGAAAACCTTGGCTTTAGCCCTCTGGAATCAGAGCTTACCCACCATAGTATATTTTGATATTAGGTGGTTCTACACATAGTTGGCAAAATGACTTGGT
AAATTTGTAATGCTGAAGTATATTAGTATAAGTTAAATTTGATGTGTCAACTTTATTTTGTATTTCCTTCCATTTGGAAGGTTTGTTAGACCATTAAGGT
TATATTAAAGTACTCTTGTGTGTGCT
  • FGFR1OP.u.f.10 (forward primer)
  • FGFR1OP.u.r.12 (reverse primer)
  • Single exon skipping

Frame 0

VGRVARARSVPCCGRWPLARLRRLSWRSKMAAT
AAAVVAEEDTELRDLLVQTLENSGVLNRIKAELR
AAVFLALEEQEKVENKTPLVNESLKKFLNTKDG
RLVASLVAEFLQFFNLDFTLAVFQPETSTLQGL
EGRENLARDLGIIEAEGTVGGPLLLEVIRRCQQK
EKGPTTGEGALDLSDVHSPPKSPEGKTSAQTTP
SKKANDEANQSDTSVSLSEPKSKSSLHLLSHET
KIGSFLSNRTLDGKDKAGLCPDEDDMEGDSFFDD
PIPKPEKTYGLRKEPRKQAGSLASLSDAPPLKS
GLSSLAGAPSLKDSESKRGNTVLKDLKLISDKI
GSLGLGTGEDDDYVDDFNSTSHRSEKSEISIGEE
IEEDLSVEIDDINTSDKLDDLTQDLTVSQLSDV
ADYLEDVA*TRRRKYSN*QGQRTDRFHFFFFQT
ITQLECLLSIGALHFKNTADIF*K*FSFY*TKYF
LFEPMCGRFNILNLTVHFFMEIDYLHSVSLQGR
NP*KHQC*EHDERCQ*SRRIAQPFVCVAAGTCD
L*KPWL*PSGIRAYPP*YILILGGSTHSWQNDLV
NL*C*SILV*VKFDVSTLFCISFHLEGLLDH*G
YIKVLLCV

Frame 1

SAA*PARGAYPAAAVGR*RGFGGCLGEARWRRR
RPQWWPRRTRSCGTCWCRRWRTAGS*TASRLNS
EQLCF*H*RSKKK*RTKLL*LMRA*KSF*IPKTV
V*WLVLLQNFFSFLTLTLLWLFFNLKLAHCKVS
KVERI*PEI*V*LKQKVLWVDPYY*K*SGAVNR
KKKGQPLGKVHLIYLMYILHQSHQREKQVHRQHQ
VRRPMMRPIRVIQVSPCQNPRAKAAFTYCPMKQ
KLDLF*ATEL*MAKTKLAFVQMKMIWKEILSLM
IPFLSQRKLTV*GRNLGSKQEVWPRSRMHPP*KV
DSAPWREPLL*KTLRVKGEIQF*KI*N*SVIKL
DHLD*ELEKMMTMLMILIVPAIAQRKVR*VLVK
R*KKTFLWK*MTSIPVISLMTSHKI*LYPSSVML
RIIWKMLHRHEEGSILINKDRGLTGSIFFFSRQ
SLSWNVCSLLVPCISKTLQIFFKSNFHFTKQNT
SYLSPCVEDLIFLI*LYISLWKLIIYTQFHYREG
THENISVKSMMKGVNKAVGSRNPLCVWLLVRVI
FENLGFSPLESELTHHSIF*Y*VVLHIVGKMTW
*ICNAEVY*YKLNLMCQLYFVFPSIWKVC*TIKV
ILKYSCVC

Frame 2

RPRSPRAERTLLRPLAVSAASAVVLEKQDGGDG
GRSGGRGGHGAAGPAGADAGEQRGPEPHQG*TP
SSCVFSTRGARKSREQNSFS**EPEKVFKYQRR
SFSG*SCCRISSVF*P*LYFGCFST*N*HTARSR
RSREFSPRFRYN*SRRYCGWTLIIRSDQALSTE
RKRANHWGRCT*SI*CTFSTKVTRGKNKCTDNT
K*EGQ**GQSE*YKCLLVRTQEQKQPSLTVP*NK
NWIFSKQQNFRWQRQSWPLSR*R*YGRRFFL**
SHS*ARENLRFEEGT*EASRKSGLALGCTPLKK
WTQLPGGSPFFKRL*E*KGKYSFERSEIDQ**NW
ITWIRNWRR**LC**F**YQPSLREK*DKYW*R
DRRRPFCGNR*HQYQ**A**PHTRSDCIPAQ*C
CGLSGRCCIDTKKEVF*LTRTED*PVPFFFFPDN
HSAGMSALYWCLAFQKHCRYFLKVIFILLNKIL
PI*AHVWKI*YS*FNCTFLYGN*LSTLSFITGK
EPMKTSVLRA**KVSIKP*DRATLCVCGCWYV*S
LKTLALALWNQSLPTIVYFDIRWFYT*LAK*LG
KFVMLKYISIS*I*CVNFILYFLPFGRFVRPLR
LY*STLVCA

Sequence FGFR1OP.aAug05

GTCGGCCGCGTAGCCCGCGCGCGGAGCGTACCCTGCTGCGGCCGTTGGCCGTTAGCGCGGCTTCGGCGGTTGTCTTGGAGAAGCAAGATGGCGGCGACGG
CGGCCGCAGTGGTGGCCGAGGAGGACACGGAGCTGCGGGACCTGCTGGTGCAGACGCTGGAGAACAGCGGGGTCCTGAACCGCATCAAGGCTGAACTCCG
AGCAGCTGTGTTTTTAGCACTAGAGGAGCAAGAAAAAGTAGAGAACAAAACTCCTTTAGTTAATGAGAGCCTGAAAAAGTTTTTAAATACCAAAGACGGT
CGTTTAGTGGCTAGTCTTGTTGCAGAATTTCTTCAGTTTTTTAACCTTGACTTTACTTTGGCTGTTTTTCAACCTGAAACTAGCACACTGCAAGGTCTCG
AAGGTCGAGAGAATTTAGCCCGAGATTTAGGTATAATTGAAGCAGAAGGTACTGTGGGTGGACCCTTATTATTAGAAGTGATCAGGCGCTGTCAACAGAA
AGAAAAAGGGCCAACCACTGGGGAAGGTGCACTTGATCTATCTGATGTACATTCTCCACCAAAGTCACCAGAGGGAAAAACAAGTGCACAGACAACACCA
AGTAAGATACCAAGGTATAAAGGACAAGGTAAGAAGAAGACAAGCGGGCAGAAGGCTGGTGACAAGAAGGCCAATGATGAGGCCAATCAGAGTGATACAA
GTGTCTCCTTGTCAGAACCCAAGAGCAAAAGCAGCCTTCACTTACTGTCCCATGAAACAAAAATTGGATCTTTTCTAAGCAACAGAACTTTAGATGGCAA
AGACAAAGCTGGCCTTTGTCCAGATGAAGATGATATGGAAGGAGATTCTTTCTTTGATGATCCCATTCCTAAGCCAGAGAAAACTTACGGTTTGAGGAAG
GAACCTAGGAAGCAAGCAGGAAGTCTGGCCTCGCTCTCGGATGCACCCCCCTTAAAAAGTGGACTCAGCTCCCTGGCGGGAGCCCCTTCTTTAAAAGACT
CTGAGAGTAAAAGGGGAAATACAGTTTTGAAAGATCTGAAATTGATCAGTGATAAAATTGGATCACTTGGATTAGGAACTGGAGAAGATGATGACTATGT
TGATGATTTTAATAGTACCAGCCATCGCTCAGAGAAAAGTGAGATAAGTATTGGTGAAGAGATAGAAGAAGACCTTTCTGTGGAAATAGATGACATCAAT
ACCAGTGATAAGCTTGATGACCTCACACAAGATCTGACTGTATCCCAGCTCAGTGATGTTGCGGATTATCTGGAAGATGTTGCATAGACACGAAGAAGGA
AGTATTCTAATTAACAAGGACAGAGGACTGACCGGTTCCATTTTTTTTTTTTCCAGACAATCACTCAGCTGGAATGTCTGCTCTCTATTGGTGCCTTGCA
TTTCAAAAACACTGCAGATATTTTTTAAAAGTAATTTTCATTTTACTAAACAAAATACTTCCTATTTGAGCCCATGTGTGGAAGATTTAATATTCTTAAT
TTAACTGTACATTTCTTTATGGAAATTGATTATCTACACTCAGTTTCATTACAGGGAAGGAACCCATGAAAACATCAGTGTTAAGAGCATGATGAAAGGT
GTCAATAAAGCCGTAGGATCGCGCAACCCTTTGTGTGTGTGGCTGCTGGTACGTGTGATCTTTGAAAACCTTGGCTTTAGCCCTCTGGAATCAGAGCTTA
CCCACCATAGTATATTTTGATATTAGGTGGTTCTACACATAGTTGGCAAAATGACTTGGTAAATTTGTAATGCTGAAGTATATTAGTATAAGTTAAATTT
GATGTGTCAACTTTATTTTGTATTTCCTTCCATTTGGAAGGTTTGTTAGACCATTAAGGTTATATTAAAGTACTCTTGTGTGTGCT
  • FGFR1OP.u.f.10 (forward primer)
  • FGFR1OP.u.r.12 (reverse primer)
  • Single exon skipping

Frame 0

VGRVARARSVPCCGRWPLARLRRLSWRSKMAAT
AAAVVAEEDTELRDLLVQTLENSGVLNRIKAELR
AAVFLALEEQEKVENKTPLVNESLKKFLNTKDG
RLVASLVAEFLQFFNLDFTLAVFQPETSTLQGL
EGRENLARDLGIIEAEGTVGGPLLLEVIRRCQQK
EKGPTTGEGALDLSDVHSPPKSPEGKTSAQTTP
SKIPRYKGQGKKKTSGQKAGDKKANDEANQSDT
SVSLSEPKSKSSLHLLSHETKIGSFLSNRTLDGK
DKAGLCPDEDDMEGDSFFDDPIPKPEKTYGLRK
EPRKQAGSLASLSDAPPLKSGLSSLAGAPSLKD
SESKRGNTVLKDLKLISDKIGSLGLGTGEDDDYV
DDFNSTSHRSEKSEISIGEEIEEDLSVEIDDIN
TSDKLDDLTQDLTVSQLSDVADYLEDVA*TRRR
KYSN*QGQRTDRFHFFFFQTITQLECLLSIGALH
FKNTADIF*K*FSFY*TKYFLFEPMCGRFNILN
LTVHFFMEIDYLHSVSLQGRNP*KHQC*EHDER
CQ*SRRIAQPFVCVAAGTCDL*KPWL*PSGIRAY
PP*YILILGGSTHSWQNDLVNL*C*SILV*VKF
DVSTLFCISFHLEGLLDH*GYIKVLLCV

Frame 1

SAA*PARGAYPAAAVGR*RGFGGCLGEARWRRR
RPQWWPRRTRSCGTCWCRRWRTAGS*TASRLNS
EQLCF*H*RSKKK*RTKLL*LMRA*KSF*IPKTV
V*WLVLLQNFFSFLTLTLLWLFFNLKLAHCKVS
KVERI*PEI*V*LKQKVLWVDPYY*K*SGAVNR
KKKGQPLGKVHLIYLMYILHQSHQREKQVHRQHQ
VRYQGIKDKVRRRQAGRRLVTRRPMMRPIRVIQ
VSPCQNPRAKAAFTYCPMKQKLDLF*ATEL*MA
KTKLAFVQMKMIWKEILSLMIPFLSQRKLTV*GR
NLGSKQEVWPRSRMHPP*KVDSAPWREPLL*KT
LRVKGEIQF*KI*N*SVIKLDHLD*ELEKMMTM
LMILIVPAIAQRKVR*VLVKR*KKTFLWK*MTSI
PVISLMTSHKI*LYPSSVMLRIIWKMLHRHEEG
SILINKDRGLTGSIFFFSRQSLSWNVCSLLVPC
ISKTLQIFFKSNFHFTKQNTSYLSPCVEDLIFLI
*LYISLWKLIIYTQFHYREGTHENISVKSMMKG
VNKAVGSRNPLCVWLLVRVIFENLGFSPLESEL
THHSIF*Y*VVLHIVGKMTW*ICNAEVY*YKLNL
MCQLYFVFPSIWKVC*TIKVILKYSCVC

Frame 2

RPRSPRAERTLLRPLAVSAASAVVLEKQDGGDG
GRSGGRGGHGAAGPAGADAGEQRGPEPHQG*TP
SSCVFSTRGARKSREQNSFS**EPEKVFKYQRR
SFSG*SCCRISSVF*P*LYFGCFST*N*HTARSR
RSREFSPRFRYN*SRRYCGWTLIIRSDQALSTE
RKRANHWGRCT*SI*CTFSTKVTRGKNKCTDNT
K*DTKV*RTR*EEDKRAEGW*QEGQ**GQSE*YK
CLLVRTQEQKQPSLTVP*NKNWIFSKQQNFRWQ
RQSWPLSR*R*YGRRFFL**SHS*ARENLRFEE
GT*EASRKSGLALGCTPLKKWTQLPGGSPFFKRL
*E*KGKYSFERSEIDQ**NWITWIRNWRR**LC
**F**YQPSLREK*DKYW*RDRRRPFCGNR*HQ
YQ**A**PHTRSDCIPAQ*CCGLSGRCCIDTKKE
VF*LTRTED*PVPFFFFPDNHSAGMSALYWCLA
FQKHCRYFLKVIFILLNKILPI*AHVWKI*YS*
FNCTFLYGN*LSTLSFITGKEPMKTSVLRA**KV
SIKP*DRATLCVCGCWYV*SLKTLALALWNQSL
PTIVYFDIRWFYT*LAK*LGKFVMLKYISIS*I
*CVNFILYFLPFGRFVRPLRLY*STLVCA

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