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Sequences

Sequence ESR2.fAug05

ATGGATATAAAAAACTCACCATCTAGCCTTAATTCTCCTTCCTCCTACAACTGCAGTCAATCCATCTTACCCCTGGAGCACGGCTCCATATACATACCTT CCTCCTATGTAGACAGCCACCATGAATATCCAGCCATGACATTCTATAGCCCTGCTGTGATGAATTACAGCATTCCCAGCAATGTCACTAACTTGGAAGG TGGGCCTGGTCGGCAGACCACAAGCCCAAATGTGTTGTGGCCAACACCTGGGCACCTTTCTCCTTTAGTGGTCCATCGCCAGTTATCACATCTGTATGCG GAACCTCAAAAGAGTCCCTGGTGTGAAGCAAGATCGCTAGAACACACCTTACCTGTAAACAGAGAGACACTGAAAAGGAAGGTTAGTGGGAACCGTTGCG CCAGCCCTGTTACTGGTCCAGGTTCAAAGAGGGATGCTCACTTCTGCGCTGTCTGCAGCGATTACGCATCGGGATATCACTATGGAGTCTGGTCGTGTGA AGGATGTAAGGCCTTTTTTAAAAGAAGCATTCAAGGACATAATGATTATATTTGTCCAGCTACAAATCAGTGTACAATCGATAAAAACCGGCGCAAGAGC TGCCAGGCCTGCCGACTTCGGAAGTGTTACGAAGTGGGAATGGTGAAGTGTGGCTCCCGGAGAGAGAGATGTGGGTACCGCCTTGTGCGGAGACAGAGAA GTGCCGACGAGCAGCTGCACTGTGCCGGCAAGGCCAAGAGAAGTGGCGGCCACGCGCCCCGAGTGCGGGAGCTGCTGCTGGACGCCCTGAGCCCCGAGCA GCTAGTGCTCACCCTCCTGGAGGCTGAGCCGCCCCATGTGCTGATCAGCCGCCCCAGTGCGCCCTTCACCGAGGCCTCCATGATGATGTCCCTGACCAAG TTGGCCGACAAGGAGTTGGTACACATGATCAGCTGGGCCAAGAAGATTCCCGGCTTTGTGGAGCTCAGCCTGTTCGACCAAGTGCGGCTCTTGGAGAGCT GTTGGATGGAGGTGTTAATGATGGGGCTGATGTGGCGCTCAATTGACCACCCCGGCAAGCTCATCTTTGCTCCAGATCTTGTTCTGGACAGGGATGAGGG GAAATGCGTAGAAGGAATTCTGGAAATCTTTGACATGCTCCTGGCAACTACTTCAAGGTTTCGAGAGTTAAAACTCCAACACAAAGAATATCTCTGTGTC AAGGCCATGATCCTGCTCAATTCCAGTATGTACCCTCTGGTCACAGCGACCCAGGATGCTGACAGCAGCCGGAAGCTGGCTCACTTGCTGAACGCCGTGA CCGATGCTTTGGTTTGGGTGATTGCCAAGAGCGGCATCTCCTCCCAGCAGCAATCCATGCGCCTGGCTAACCTCCTGATGCTCCTGTCCCACGTCAGGCA TGCGAGGTACGCGCCCTAAGGAGCTGCTCTGCTTGGGCTTGGGATGGGATTATGTGCTCCACGGAGGGTGAAGTGATTTGGGAAAAGTGTCTGCAAGTTA AGGAAAATGAATGCCTGAAAGGGAATGGGGAATTTGTCAGTTCACACACCTGTAAGCAAAGATGGGCACAGAGTGGGCATGGAAGGAATGTCATGTGGTA TCTTACAGGCTCTGCATGGCAGCCAGTGGTGGCTCATGGGTTTTTCAATTGCTGGGGTTTATAGCCTGTTTATGGAGTCCTAAAAGGGGCAGTTCCTCCC CTAACACGAACTGCCACCCCTGTTTACACCACCCAGGGCTGAGGCCCTGAGGCCACTTTTTGTGGAGAGGCTAAGACCCGCTCCCCTAGATGGCCCCTCG AGCTGGTGATGCGAAGAAGTGCACAAATGCTTCCCTAAGAGTTGTTCTTTCGGTGGCATCAGGAAATTAAGGATAAGACTTAAGAGAAGTGGTGGACCCA GCAGATTTAGGAAGGCAGGGCTGTAGGTAGGGCATGTTTCTGATCAGGAAACGTAATTGTGTGTGCTGATGAAGAGGGTGTGCAGTGGTGGCTACTGTTG GTACAATGATGCTCAGTGCTTGGTGTCACCCACGATGAGGGTAGCCTTGCCCTGGAGCTGGAGGAGGGGAGGGGAGGGTGGAAGGTAATTAACTGGTCAC TGAGGAGGCAAGTCTAGAGGCTGTGGAGAAGGACAATATACACCTCGAGAATCTTAAGTGAGATGAAGACCTCTGCCTTTCCCCTTTAATGATTGCTCAG CACATAGCCATTTGCAGAACAGATCCTGTGTTTGTAGATTCCTTCATTGTGAATTTATCTGCTTGCTAAAATTTATTTGTAACCCCAAAATCAATATTTG TGGTGTTTTTGAGGTCATGAACAGAGTGGCAGAAATTTTGAGTTGCCCTTTATGTACAGTCCCAGCTGAGATGGAACAAGCAGCTGCTCTCATACTGTCA ACAAGTGTCCTTTACTTGGTCTACTTAGTGCCATGGTTTTACATTTTTGTGCTTTTGGTGACTTCACTGTTTAAAATGCCCCCCTGGTGTGGTGCTGAAG ACCTGTCTAGTGTTCCTCGGTGTGAAAAAGCTGTGATGTGCCTTATGGAGAAAGTATGTGTTAAGCTTTGCTCGGGTGTGAGTTATAGTGCTGCTGGCCA TGAGTTCAATGTTAATGAGTCAATGGTATTTATCACATAAGGCATCTTTAGAAAGAAACACACATAAAACAAGGTTTTGTATTGATCAGCTGATGAAGAT GTGGCCAGAGGCTTGCAGGAACCTAACCCTGTATTTCCCCTATGAGTGAGGATTCAGTGTTCACAGTGACTTTACGGAACATAATTACCGCAAACAATGA GGATTGATTGTCCTATGTGTCAGGCCATTGTAGGTGTGTGGTGGGACACAGAGGCTGACAAGACATCGTCCTTGCCCTTGAGCCTAAATTATCAGGGGGA GCTGGATGCACGAGCCATGGATAAATGGGCTGGGGGAAGAGTGGGTTTAGGGGTGGGGTAGACTGGCTCTGAGCAAAGAGAGCCGGGGAAGGCTTCGGGG TTCCTGTGGCTGCCTCGGAGGAGGGAATCTCAGCACCTTTTTGTCCCCATAGTAACAAGGGCATGGAACATCTGCTCAACATGAAGTGCAAAAATGTGGT CCCAGTGTATGACCTGCTGCTGGAGATGCTGAATGCCCACGTGCTTCGCGGGTGCAAGTCCTCCATCACGGGGTCCGAGTGCAGCCCGGCAGAGGACAGT AAAAGCAAAGAGGGCTCCCAGAACCCACAGTCTCAGTGACGCCTGGCCCTGAGGTGAACTGGCCCACAGAGGTCACAGGCTGAAGCGTGAACTCCAGTGT GTCAGGAGCCTGGGCTTCATCTTTCTGCTGTGTGGTCCCTCATTTGGTGATGGCAGGCTTGGTCATGTACCATCCTTCCCTCCACCTTCCCAACTCTCAG GAGTCGGTGTGAGGAAGCCATAGTTTCCCTTGTTAGCAGAGGGCACATTTGAATGCAGCGTTTCCACACTCAATGGCCTCATAGGATCTCAGTGTGGTCT TTCTTACTTTCCTTCTTCCTTCCTCCCCTTTGTGAAACATCTTAAAGGTTTTGGAATGAATGGTGGAAATCTGACTTGGAAGGGCTGCGAATCAGAAAGG GGAGAGGAAGTGACACGCTTACAGAAGTGGGCTAACCCTTCTTGTGTGGCACACACTACCCTTCCCTCTGAGAGTTGACCTTTGCTGTTTTCCGGACCAC TCCATTGTAAGATTGAAAACCCCTGTGGCAATTGCGTACTTACCTCCCAGGCCTGTGGGGACTGATCATATCATATGATGCTTATTCTGTCAAAGGCCAG AGGGACTGTGGTTAAGCTGGGATGTGAGTCATGTTCTCTCCCTGACCTTGCTGCCAGCTGCACACAGATTTGTCCCTCTCGATTTGTATTCACAGAGCCT GCCAATAATTTGGGGTATGTGTGTATGAGCGTGTGATCATTTTCATGCAGGACTGTGGGAGATACAAATCTCGCTGCTTCTGGAGCTGCTCTTCCTTAAA CCTGTTGTCCCATGGGGCCAGCGTGGGTGCTGGAGAAAGGCCGTGTTTGCAGGAATGGGGTTCTCTCCTGTGGGTGTGGGTGACAGCCACAGTGTTTCCC TGGGGCAATGTGGATGCAGTTTCCATCTTGTACAACCTCATAAGTAGCAGCCACAATTGCCCCATCAGTCACCACAAGTAGTCAGGGATACTTTGGGCTG TGGATGTGTGCAGTGTGCTGTTTTATGGATGGATGAGTAGCTATGCACCCCAGTGTGTCAGCTCTGGGGCCACACTGTATAGCCTTGATGAGTACGCCCC TTGAACAAGACCCAGTTTGTGAACTCTCCTTAAAGAGAAATATTTAGGGATAATTATTTATAGCAAGAAAGAATTCTTTTACACTTGAGAGCTCTTTTAA AAATATTTTCTTATTGGAAAATTTATATGGTGGGCAGGGTGAAAAAGAAACAGTAAAAATATTAGTTCTTATTCCAAGTGGAACATAAATAGGACATGAA GAAGGGCACCTCTGAAATGACAACTTTAACTCACCTTTTAAAAGATGTGAAATTTCCAGTTTTGGATACACGGTGAATATGTAAAATGAGTAACAGCATA CTATGGAAGCCAGCAATTAAATAATCATGTTTCATTATTGCAGTAACGTTTTAAACAATTACCTTGTGATATGATATTAAATATATTTTCTTTTTGAAAA TATGTTCACTTTGGGTAGCACATCCTGTATTTACTAAGTCATTAGGAAGACTGCATTCAGTGTTACCAAGACTGGTTTTTGCTAGTAAGACCTCGAATAA TCCATAATTTTGATATTGGTGCAATTTTACTATAAGTTGAGCTTAGCTGTTTCAGAAATGCTTGGACAAGTACCTAGAGAACACACTGATGTCTGTGTTC TGAGGCAGTCTGAAGTTATTCTTAGAGACTCAGTTACAGCTTTAGTAAGATTTAGTACAGGCAGGATAAGCTTGGTTTCATAGGAACCAGGGAACCAGTG TTAGTGTCAGCTTCTTTCCTCCTGGTCAGCCTAGAATCCCCCACTCCCAATAGAGGGGTTTGGAAGCTGGAGAGTAGGAAGTAAGAGGCAAAGAAGGCAG CCTTCAGCAACTCATTATCTGCCAGTGAAATTCTATTAAATGTATTTTTAAAAGAGATTACCAGGTAACAAAAACATAAAAAACCAAAACAAGGCCAGAT GTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGAACCACTTGAGCCCATGAGTTTGACTCCAGGCTGGGCAACATGGAAACCC TGTCCTACAAAAGATACAAAAATTAGCCAGGCGTGGTGGTGCAGGCCTGTAGTTCCAGCTACCTGGGAGGCTGAGGTGGGAGGATCACCTGAGCCTGGGG AGATCAAGGCTGCAGTCCATTGCACTCCAGCCTGGGTGACAGAGGGAGACCCTGTCTC

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIP SSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEG GPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYA EPQKSPWCEARSLEHTLPVNRETLKRKVSGNRC ASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCE GCKAFFKRSIQGHNDYICPATNQCTIDKNRRKS CQACRLRKCYEVGMVKCGSRRERCGYRLVRRQR SADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQ LVLTLLEAEPPHVLISRPSAPFTEASMMMSLTK LADKELVHMISWAKKIPGFVELSLFDQVRLLES CWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEG KCVEGILEIFDMLLATTSRFRELKLQHKEYLCV KAMILLNSSMYPLVTATQDADSSRKLAHLLNAV TDALVWVIAKSGISSQQQSMRLANLLMLLSHVRH ARYAP*GAALLGLGMGLCAPRRVK*FGKSVCKL RKMNA*KGMGNLSVHTPVSKDGHRVGMEGMSCG ILQALHGSQWWLMGFSIAGVYSLFMES*KGQFLP *HELPPLFTPPRAEALRPLFVERLRPAPLDGPS SW*CEEVHKCFPKSCSFGGIRKLRIRLKRSGGP SRFRKAGL*VGHVSDQET*LCVLMKRVCSGGYCW YNDAQCLVSPTMRVALPWSWRRGGEGGR*LTGH *GGKSRGCGEGQYTPRES*VR*RPLPFPFNDCS AHSHLQNRSCVCRFLHCEFICLLKFICNPKINIC GVFEVMNRVAEILSCPLCTVPAEMEQAAALILS TSVLYLVYLVPWFYIFVLLVTSLFKMPPWCGAE DLSSVPRCEKAVMCLMEKVCVKLCSGVSYSAAGH EFNVNESMVFIT*GIFRKKHT*NKVLY*SADED VARGLQEPNPVFPL*VRIQCSQ*LYGT*LPQTM RIDCPMCQAIVGVWWDTEADKTSSLPLSLNYQGE LDARAMDKWAGGRVGLGVG*TGSEQREPGKASG FLWLPRRRESQHLFVPIVTRAWNICST*SAKMW SQCMTCCWRC*MPTCFAGASPPSRGPSAARQRTV KAKRAPRTHSLSDAWP*GELAHRGHRLKRELQC VRSLGFIFLLCGPSFGDGRLGHVPSFPPPSQLS GVGVRKP*FPLLAEGTFECSVSTLNGLIGSQCGL SYFPSSFLPFVKHLKGFGMNGGNLTWKGCESER GEEVTRLQKWANPSCVAHTTLPSES*PLLFSGP LHCKIENPCGNCVLTSQACGD*SYHMMLILSKAR GTVVKLGCESCSLPDLAASCTQICPSRFVFTEP ANNLGYVCMSV*SFSCRTVGDTNLAASGAALP* TCCPMGPAWVLEKGRVCRNGVLSCGCG*QPQCFP GAMWMQFPSCTTS*VAATIAPSVTTSSQGYFGL WMCAVCCFMDG*VAMHPSVSALGPHCIALMSTP LEQDPVCELSLKRNI*G*LFIARKNSFTLESSFK NIFLLENLYGGQGEKETVKILVLIPSGT*IGHE EGHL*NDNFNSPFKRCEISSFGYTVNM*NE*QH TMEASN*IIMFHYCSNVLNNYLVI*Y*IYFLFEN MFTLGSTSCIY*VIRKTAFSVTKTGFC**DLE* SIILILVQFYYKLSLAVSEMLGQVPREHTDVCV LRQSEVILRDSVTALVRFSTGRISLVS*EPGNQC *CQLLSSWSA*NPPLPIEGFGSWRVGSKRQRRQ PSATHYLPVKFY*MYF*KRLPGNKNIKNQNKAR CGGSRL*SQHFGRPRWANHLSP*V*LQAGQHGNP VLQKIQKLARRGGAGL*FQLPGRLRWEDHLSLG RSRLQSIALQPG*QRETLS

Frame 1

WI*KTHHLALILLPPTTAVNPSYPWSTAPYTYL PPM*TATMNIQP*HSIALL**ITAFPAMSLTWK VGLVGRPQAQMCCGQHLGTFLL*WSIASYHICMR NLKRVPGVKQDR*NTPYL*TERH*KGRLVGTVA PALLLVQVQRGMLTSALSAAITHRDITMESGRV KDVRPFLKEAFKDIMIIFVQLQISVQSIKTGARA ARPADFGSVTKWEW*SVAPGERDVGTALCGDRE VPTSSCTVPARPREVAATRPECGSCCWTP*APS S*CSPSWRLSRPMC*SAAPVRPSPRPP**CP*PS WPTRSWYT*SAGPRRFPALWSSACSTKCGSWRA VGWRC**WG*CGAQLTTPASSSLLQILFWTGMR GNA*KEFWKSLTCSWQLLQGFES*NSNTKNISVS RP*SCSIPVCTLWSQRPRMLTAAGSWLTC*TP* PMLWFG*LPRAASPPSSNPCAWLTS*CSCPTSG MRGTRPKELLCLGLGWDYVLHGG*SDLGKVSAS* GK*MPEREWGICQFTHL*AKMGTEWAWKECHVV SYRLCMAASGGSWVFQLLGFIACLWSPKRGSSS PNTNCHPCLHHPGLRP*GHFLWRG*DPLP*MAPR AGDAKKCTNASLRVVLSVASGN*G*DLREVVDP ADLGRQGCR*GMFLIRKRNCVC**RGCAVVATV GTMMLSAWCHPR*G*PCPGAGGGEGRVEGN*LVT EEASLEAVEKDNIHLENLK*DEDLCLSPLMIAQ HIAICRTDPVFVDSFIVNLSAC*NLFVTPKSIF VVFLRS*TEWQKF*VALYVQSQLRWNKQLLSYCQ QVSFTWST*CHGFTFLCFW*LHCLKCPPGVVLK TCLVFLGVKKL*CALWRKYVLSFARV*VIVLLA MSSMLMSQWYLSHKASLERNTHKTRFCIDQLMKM WPEACRNLTLYFPYE*GFSVHSDFTEHNYRKQ* GLIVLCVRPL*VCGGTQRLTRHRPCP*A*IIRG SWMHEPWINGLGEEWV*GWGRLALSKESRGRLRG SCGCLGGGNLSTFLSP**QGHGTSAQHEVQKCG PSV*PAAGDAECPRASRVQVLHHGVRVQPGRGQ *KQRGLPEPTVSVTPGPEVNWPTEVTG*SVNSSV SGAWASSFCCVVPHLVMAGLVMYHPSLHLPNSQ ESV*GSHSFPC*QRAHLNAAFPHSMAS*DLSVV FLTFLLPSSPL*NILKVLE*MVEI*LGRAANQKG ERK*HAYRSGLTLLVWHTLPFPLRVDLCCFPDH SIVRLKTPVAIAYLPPRPVGTDHII*CLFCQRP EGLWLSWDVSHVLSLTLLPAAHRFVPLDLYSQSL PIIWGMCV*ACDHFHAGLWEIQISLLLELLFLK PVVPWGQRGCWRKAVFAGMGFSPVGVGDSHSVS LGQCGCSFHLVQPHK*QPQLPHQSPQVVRDTLGC GCVQCAVLWMDE*LCTPVCQLWGHTV*P**VRP LNKTQFVNSP*REIFRDNYL*QERILLHLRALL KIFSYWKIYMVGRVKKKQ*KY*FLFQVEHK*DMK KGTSEMTTLTHLLKDVKFPVLDTR*ICKMSNSI LWKPAIK*SCFIIAVTF*TITL*YDIKYIFFLK ICSLWVAHPVFTKSLGRLHSVLPRLVFASKTSNN P*F*YWCNFTIS*A*LFQKCLDKYLENTLMSVF *GSLKLFLETQLQL**DLVQAG*AWFHRNQGTS VSVSFFPPGQPRIPHSQ*RGLEAGE*EVRGKEGS LQQLIICQ*NSIKCIFKRDYQVTKT*KTKTRPD VVAHACNPSTLGGRGGRTT*AHEFDSRLGNMET LSYKRYKN*PGVVVQACSSSYLGG*GGRIT*AWG DQGCSPLHSSLGDRGRPCL

Frame 2

GYKKLTI*P*FSFLLQLQSIHLTPGARLHIHTF LLCRQPP*ISSHDIL*PCCDELQHSQQCH*LGR WAWSADHKPKCVVANTWAPFSFSGPSPVITSVC GTSKESLV*SKIARTHLTCKQRDTEKEG*WEPLR QPCYWSRFKEGCSLLRCLQRLRIGISLWSLVV* RM*GLF*KKHSRT**LYLSSYKSVYNR*KPAQE LPGLPTSEVLRSGNGEVWLPEREMWVPPCAETEK CRRAAALCRQGQEKWRPRAPSAGAAAGRPEPRA ASAHPPGG*AAPCADQPPQCALHRGLHDDVPDQ VGRQGVGTHDQLGQEDSRLCGAQPVRPSAALGEL LDGGVNDGADVALN*PPRQAHLCSRSCSGQG*G EMRRRNSGNL*HAPGNYFKVSRVKTPTQRISLC QGHDPAQFQYVPSGHSDPGC*QQPEAGSLAERRD RCFGLGDCQERHLLPAAIHAPG*PPDAPVPRQA CEVRALRSCSAWAWDGIMCSTEGEVIWEKCLQV KENECLKGNGEFVSSHTCKQRWAQSGHGRNVMWY LTGSAWQPVVAHGFFNCWGL*PVYGVLKGAVPP LTRTATPVYTTQG*GPEATFCGEAKTRSPRWPL ELVMRRSAQMLP*ELFFRWHQEIKDKT*EKWWTQ QI*EGRAVGRACF*SGNVIVCADEEGVQWWLLL VQ*CSVLGVTHDEGSLALELEEGRGGWKVINWS LRRQV*RLWRRTIYTSRILSEMKTSAFPL**LLS T*PFAEQILCL*IPSL*IYLLAKIYL*PQNQYL WCF*GHEQSGRNFELPFMYSPS*DGTSSCSHTV NKCPLLGLLSAMVLHFCAFGDFTV*NAPLVWC*R PV*CSSV*KSCDVPYGESMC*ALLGCEL*CCWP *VQC**VNGIYHIRHL*KETHIKQGFVLIS**R CGQRLAGT*PCISPMSEDSVFTVTLRNIITANNE D*LSYVSGHCRCVVGHRG*QDIVLALEPKLSGG AGCTSHG*MGWGKSGFRGGVDWL*AKRAGEGFG VPVAASEEGISAPFCPHSNKGMEHLLNMKCKNVV PVYDLLLEMLNAHVLRGCKSSITGSECSPAEDS KSKEGSQNPQSQ*RLALR*TGPQRSQAEA*TPV CQEPGLHLSAVWSLIW*WQAWSCTILPSTFPTLR SRCEEAIVSLVSRGHI*MQRFHTQWPHRISVWS FLLSFFLPPLCETS*RFWNEWWKSDLEGLRIRK GRGSDTLTEVG*PFLCGTHYPSL*ELTFAVFRTT PL*D*KPLWQLRTYLPGLWGLIISYDAYSVKGQ RDCG*AGM*VMFSP*PCCQLHTDLSLSICIHRA CQ*FGVCVYERVIIFMQDCGRYKSRCFWSCSSLN LLSHGASVGAGERPCLQEWGSLLWVWVTATVFP WGNVDAVSILYNLISSSHNCPISHHK*SGILWA VDVCSVLFYGWMSSYAPQCVSSGATLYSLDEYAP *TRPSL*TLLKEKYLGIIIYSKKEFFYT*ELF* KYFLIGKFIWWAG*KRNSKNISSYSKWNINRT* RRAPLK*QL*LTF*KM*NFQFWIHGEYVK*VTAY YGSQQLNNHVSLLQ*RFKQLPCDMILNIFSF*K YVHFG*HILYLLSH*EDCIQCYQDWFLLVRPRI IHNFDIGAILL*VELSCFRNAWTST*RTH*CLCS EAV*SYS*RLSYSFSKI*YRQDKLGFIGTREPV LVSASFLLVSLESPTPNRGVWKLESRK*EAKKA AFSNSLSASEILLNVFLKEITR*QKHKKPKQGQM WWLTPVIPALWEAEVGEPLEPMSLTPGWATWKP CPTKDTKISQAWWCRPVVPATWEAEVGGSPEPG EIKAAVHCTPAWVTEGDPV

Sequence ESR2.eAug05

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

Frame 1

Frame 2

Sequence ESR2.dAug05

GGCGGCGAGTCTCCCGGATGCTCCTCAGCTCTGGGGACGCGGTGCAGAAGTGTGAGGGCGCCCGGCTTCCAGGCAGTAATGGGCGGGTCCCTGCGCGGGA GCGTGGCGGGCGCTGGACTCTACAGCAGATGTGGAACTGGAGAGCTTGGCGCGCCTTCCGACTTTGTCACACACCTGCGCCGCCAGACTGGGGTCGGGCC CCTCCGCGTTCTGCTCTGGAGTGCCTGGGTCTGGGCCCAGCACCGCGCTTTTAGAATCTCCTCAGCTGAATCTGACGCTCAGCAGTGGGTGAAGCGCAGC CCCCTGTTTCAGGCCCTGCCGAGCTGGAAGGAGTGTCAGAGCTGGAGCGCGCGTGGCCCCCTCTGTGTTGGGGTCACCCCGGGGTTGCCAGGGCTCAGGG AGGGTCGTAGTCTGGATTTTGTCACCCGCACGTCCCCACCCCCCAGCAGGTCTGGGGTTGGAGAATCCACGCGGGCTTCATAAGCTAGATGCCAGTTAAC TGTCGAGAGGGGACGCTCCCTCCTCGTAGGCGTCCACACTGGAGAAGGAATAAGATGGGCGATTGCCTGGGAAGCCTGACAGGGCGGCGGCAGCTGGGAT GCTGGAGAGGACTGGCCCCTTGAGTTACTGAGTCCGATGAATGTGCTTGCTCTGCTGGAGGAACCGCGCTCAGGTTACAGTCATCCCAATATGGTTCTGA AGACATCCAAGTGGAGATATGGCATTTAAATTCATGAGATTGGATGAGATCCCACCAAAGGAACAGGTTTAGGTGGAGACAACCAAATACCGATGCCTAG GACACTGCAGTGTTTAGAATTCAAGGAGATGAGAAGGAAACAGGAGGGAAGATTGAAAAGAAGAGTCCAGTGTGTTATGAGGAAAACCCCAAGAGCATGC TGCCTTACAAGACAGGTGAAAAATGTGTTCTGTGAAAGAAAGAGTAATTAACTGTTAAATGTTACAGACTGATCAAATAAAATGAAGACTGAGAATGGCC TGTTTGTAAGAGGGAGACATCAACCTGTTGTGGAAAAGAATGATCACTTAAAGTCTTTAGAAATTCTGAACCAACTCTCTAGCAGGTGATCCTTGTTAGA ATTTGAGCCCTTAACGCTATCCAGGACTGGAGGTTGAAGGGACGATAGAGGGAGCAGGAGGAGAATGCACATGGATTAAGGAGCGAGAACACAGAAATCC TGGGCTCTCTTCTCCCAGCCACAAGGTTAGGTTGAAAAACAGAGCAGATGGAGGTAGTTTGTAGCCTACAGGTGCCCTGAATGAAGCTTCCACAGTGCTA AAGTGGAAGAACGAGGGACTCCAAGGGAAGGATTCAAGGCTGGGCCCATGCACCTGTGTAATTCAGAAGAGACCCCAGAGGAGATCAGCGCCCTCTAATT AGCCCTGTATCTGGGCTCTACAGGACAGACATGCCTCCATTTATGCAACAAATAAGAACAGCATCTCATGACAGTGGAGAAAACATGGGATGTGCAGGTA GGGTTTTGTTTTGCCTCTTGGTAGTTTCTTTCCTACGGAAAATTCTCCCTCTGATCTTTCCAAGTCAAAGGCTTCAGCAAACATTTGTTGAACGCGTGGA TTGTGCTAGGTGGGTGTTATGGACCATGGAGAATGCTAGAGATGTAAGACATGCGCTGTCCAATCGCAGCGCAGGTTGTGTTGACAGCCATTATACTTGC CCACGAATCTTTGAGAACATTATAATGACCTTTGTGCCTCTTCTTGCAAGGTGTTTTCTCAGCTGTTATCTCAAGACATGGATATAAAAAACTCACCATC TAGCCTTAATTCTCCTTCCTCCTACAACTGCAGTCAATCCATCTTACCCCTGGAGCACGGCTCCATATACATACCTTCCTCCTATGTAGACAGCCACCAT GAATATCCAGCCATGACATTCTATAGCCCTGCTGTGATGAATTACAGCATTCCCAGCAATGTCACTAACTTGGAAGGTGGGCCTGGTCGGCAGACCACAA GCCCAAATGTGTTGTGGCCAACACCTGGGCACCTTTCTCCTTTAGTGGTCCATCGCCAGTTATCACATCTGTATGCGGAACCTCAAAAGAGTCCCTGGTG TGAAGCAAGATCGCTAGAACACACCTTACCTGTAAACAGAGAGACACTGAAAAGGAAGGTTAGTGGGAACCGTTGCGCCAGCCCTGTTACTGGTCCAGGT TCAAAGAGGGATGCTCACTTCTGCGCTGTCTGCAGCGATTACGCATCGGGATATCACTATGGAGTCTGGTCGTGTGAAGGATGTAAGGCCTTTTTTAAAA GAAGCATTCAAGGACATAATGATTATATTTGTCCAGCTACAAATCAGTGTACAATCGATAAAAACCGGCGCAAGAGCTGCCAGGCCTGCCGACTTCGGAA GTGTTACGAAGTGGGAATGGTGAAGTGTGGCTCCCGGAGAGAGAGATGTGGGTACCGCCTTGTGCGGAGACAGAGAAGTGCCGACGAGCAGCTGCACTGT GCCGGCAAGGCCAAGAGAAGTGGCGGCCACGCGCCCCGAGTGCGGGAGCTGCTGCTGGACGCCCTGAGCCCCGAGCAGCTAGTGCTCACCCTCCTGGAGG CTGAGCCGCCCCATGTGCTGATCAGCCGCCCCAGTGCGCCCTTCACCGAGGCCTCCATGATGATGTCCCTGACCAAGTTGGCCGACAAGGAGTTGGTACA CATGATCAGCTGGGCCAAGAAGATTCCCGGCTTTGTGGAGCTCAGCCTGTTCGACCAAGTGCGGCTCTTGGAGAGCTGTTGGATGGAGGTGTTAATGATG GGGCTGATGTGGCGCTCAATTGACCACCCCGGCAAGCTCATCTTTGCTCCAGATCTTGTTCTGGACAGGGATGAGGGGAAATGCGTAGAAGGAATTCTGG AAATCTTTGACATGCTCCTGGCAACTACTTCAAGGTTTCGAGAGTTAAAACTCCAACACAAAGAATATCTCTGTGTCAAGGCCATGATCCTGCTCAATTC CAGTATGTACCCTCTGGTCACAGCGACCCAGGATGCTGACAGCAGCCGGAAGCTGGCTCACTTGCTGAACGCCGTGACCGATGCTTTGGTTTGGGTGATT GCCAAGAGCGGCATCTCCTCCCAGCAGCAATCCATGCGCCTGGCTAACCTCCTGATGCTCCTGTCCCACGTCAGGCATGCGAGGGCAGAAAAGGCCTCTC AAACACTCACCTCATTTGGAATGAAGATGGAGACTCTTTTGCCTGAAGCAACGATGGAGCAGTGACCCTCTAATCAACTCGGTGGCCTAAAGAAAAATCT TGGGTAACATTTTCACTTCAGTTTCCCTCTGGGATCATTGTAATCCATGAAAAAAATAATTTTAAAGAAAGAGTTAAAATACTTTGAAGTTAGTTATGTG GTTAAAAACCACCTTCCTTTCTATTATCAATCCAACAATTTGATAACTGTAAACGCTAAAGTGAAGACGGATTCTCTTCAGATGGTCTCCTTAACTGCCC AGGGCTTGCAGATGTCTCACCCATGAGGGGCACCAATGTAGAAAGCTGAGGCTTCATCTACTGATGAGCTTCACTGGTTTCCCCTGAGGTTTGTGCTTTG GCAGAGAAGGGGAGGAGGGGACTGGGATTGTGTGGTCAGCTGTGCCTGCCAACAGATGCAGGTTAGGAACTGTGTTCAGTATCTTCCAATAAGAAAGGGG AAATGCCGATGCCTATCCTCTTTGTTTAGGTAGAAAGTAAAATGCTACTGGACTTAAATGGGCAACAAGGGGCTTTGCCTGTTCATTTGCCATGGAGAGG GCTGGGAATCCAGGTGCGGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCGAGGTGGGCAGATCAGTTGAGGTCAGGAGTTTGAAACCAGCCTGG CCAACATGGCGAAACCCCGTCTCTATTAAAAATATAATAATTAGCCAGGCATGGTGGTGTGTGCTTGTAATCCCAGCTACTCAGGAGGCTGAGGCATGAG AATGGCTTGAACCTGGAAGGCAAAGGTTGCAGTGAGCCGAGATTGGGCCACCGCACTCCAGCCTGGGTGACTGACAGAGTGAGACTCTGTCAAAAA

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

GGESPGCSSALGTRCRSVRAPGFQAVMGGSLRG SVAGAGLYSRCGTGELGAPSDFVTHLRRQTGVGP LRVLLWSAWVWAQHRAFRISSAESDAQQWVKRS PLFQALPSWKECQSWSARGPLCVGVTPGLPGLR EGRSLDFVTRTSPPPSRSGVGESTRAS*ARCQLT VERGRSLLVGVHTGEGIRWAIAWEA*QGGGSWD AGEDWPLELLSPMNVLALLEEPRSGYSHPNMVL KTSKWRYGI*IHEIG*DPTKGTGLGGDNQIPMPR TLQCLEFKEMRRKQEGRLKRRVQCVMRKTPRAC CLTRQVKNVFCERKSN*LLNVTD*SNKMKTENG LFVRGRHQPVVEKNDHLKSLEILNQLSSR*SLLE FEPLTLSRTGG*RDDRGSRRRMHMD*GARTQKS WALFSQPQG*VEKQSRWR*FVAYRCPE*SFHSA KVEERGTPREGFKAGPMHLCNSEETPEEISAL*L ALYLGSTGQTCLHLCNK*EQHLMTVEKTWDVQV GFCFASW*FLSYGKFSL*SFQVKGFSKHLLNAW IVLGGCYGPWRMLEM*DMRCPIAAQVVLTAIILA HESLRTL**PLCLFLQGVFSAVISRHGYKKLTI *P*FSFLLQLQSIHLTPGARLHIHTFLLCRQPP *ISSHDIL*PCCDELQHSQQCH*LGRWAWSADHK PKCVVANTWAPFSFSGPSPVITSVCGTSKESLV *SKIARTHLTCKQRDTEKEG*WEPLRQPCYWSR FKEGCSLLRCLQRLRIGISLWSLVV*RM*GLF*K KHSRT**LYLSSYKSVYNR*KPAQELPGLPTSE VLRSGNGEVWLPEREMWVPPCAETEKCRRAAAL CRQGQEKWRPRAPSAGAAAGRPEPRAASAHPPGG *AAPCADQPPQCALHRGLHDDVPDQVGRQGVGT HDQLGQEDSRLCGAQPVRPSAALGELLDGGVND GADVALN*PPRQAHLCSRSCSGQG*GEMRRRNSG NL*HAPGNYFKVSRVKTPTQRISLCQGHDPAQF QYVPSGHSDPGC*QQPEAGSLAERRDRCFGLGD CQERHLLPAAIHAPG*PPDAPVPRQACEGRKGLS NTHLIWNEDGDSFA*SNDGAVTL*STRWPKEKS WVTFSLQFPSGIIVIHEKNNFKERVKIL*S*LC G*KPPSFLLSIQQFDNCKR*SEDGFSSDGLLNCP GLADVSPMRGTNVES*GFIY**ASLVSPEVCAL AEKGRRGLGLCGQLCLPTDAG*ELCSVSSNKKG EMPMPILFV*VESKMLLDLNGQQGALPVHLPWRG LGIQVRWLTPVIPTLWEAEVGRSVEVRSLKPAW PTWRNPVSIKNIIISQAWWCVLVIPATQEAEA* EWLEPGRQRLQ*AEIGPPHSSLGD*QSETLSK

Frame 1

AASLPDAPQLWGRGAEV*GRPASRQ*WAGPCAG AWRALDSTADVELESLARLPTLSHTCAARLGSG PSAFCSGVPGSGPSTALLESPQLNLTLSSG*SAA PCFRPCRAGRSVRAGARVAPSVLGSPRGCQGSG RVVVWILSPARPHPPAGLGLENPRGLHKLDAS* LSRGDAPSS*ASTLEKE*DGRLPGKPDRAAAAGM LERTGPLSY*VR*MCLLCWRNRAQVTVIPIWF* RHPSGDMAFKFMRLDEIPPKEQV*VETTKYRCL GHCSV*NSRR*EGNRRED*KEESSVL*GKPQEHA ALQDR*KMCSVKERVINC*MLQTDQIK*RLRMA CL*EGDINLLWKRMIT*SL*KF*TNSLAGDPC* NLSP*RYPGLEVEGTIEGAGGECTWIKEREHRNP GLSSPSHKVRLKNRADGGSL*PTGALNEASTVL KWKNEGLQGKDSRLGPCTCVIQKRPQRRSAPSN *PCIWALQDRHASIYATNKNSIS*QWRKHGMCR* GFVLPLGSFFPTENSPSDLSKSKASANIC*TRG LC*VGVMDHGEC*RCKTCAVQSQRRLC*QPLYL PTNL*EHYNDLCASSCKVFSQLLSQDMDIKNSPS SLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHH EYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTT SPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWC EARSLEHTLPVNRETLKRKVSGNRCASPVTGPG SKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFK RSIQGHNDYICPATNQCTIDKNRRKSCQACRLRK CYEVGMVKCGSRRERCGYRLVRRQRSADEQLHC AGKAKRSGGHAPRVRELLLDALSPEQLVLTLLE AEPPHVLISRPSAPFTEASMMMSLTKLADKELVH MISWAKKIPGFVELSLFDQVRLLESCWMEVLMM GLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGIL EIFDMLLATTSRFRELKLQHKEYLCVKAMILLNS SMYPLVTATQDADSSRKLAHLLNAVTDALVWVI AKSGISSQQQSMRLANLLMLLSHVRHARAEKAS QTLTSFGMKMETLLPEATMEQ*PSNQLGGLKKNL G*HFHFSFPLGSL*SMKKIILKKELKYFEVSYV VKNHLPFYYQSNNLITVNAKVKTDSLQMVSLTA QGLQMSHP*GAPM*KAEASSTDELHWFPLRFVLW QRRGGGDWDCVVSCACQQMQVRNCVQYLPIRKG KCRCLSSLFR*KVKCYWT*MGNKGLCLFICHGE GWESRCGGSHL*SQHFGRPRWADQLRSGV*NQPG QHGETPSLLKI**LARHGGVCL*SQLLRRLRHE NGLNLEGKGCSEPRLGHRTPAWVTDRVRLCQK

Frame 2

RRVSRMLLSSGDAVQKCEGARLPGSNGRVPARE RGGRWTLQQMWNWRAWRAFRLCHTPAPPDWGRA PPRSALECLGLGPAPRF*NLLS*I*RSAVGEAQ PPVSGPAELEGVSELERAWPPLCWGHPGVARAQG GS*SGFCHPHVPTPQQVWGWRIHAGFIS*MPVN CREGTLPPRRRPHWRRNKMGDCLGSLTGRRQLG CWRGLAP*VTESDECACSAGGTALRLQSSQYGSE DIQVEIWHLNS*DWMRSHQRNRFRWRQPNTDA* DTAVFRIQGDEKETGGKIEKKSPVCYEENPKSM LPYKTGEKCVL*KKE*LTVKCYRLIK*NED*EWP VCKRETSTCCGKE*SLKVFRNSEPTL*QVILVR I*ALNAIQDWRLKGR*REQEENAHGLRSENTEI LGSLLPATRLG*KTEQMEVVCSLQVP*MKLPQC* SGRTRDSKGRIQGWAHAPV*FRRDPRGDQRPLI SPVSGLYRTDMPPFMQQIRTASHDSGENMGCAG RVLFCLLVVSFLRKILPLIFPSQRLQQTFVERVD CARWVLWTMENARDVRHALSNRSAGCVDSHYTC PRIFENIIMTFVPLLARCFLSCYLKTWI*KTHH LALILLPPTTAVNPSYPWSTAPYTYLPPM*TATM NIQP*HSIALL**ITAFPAMSLTWKVGLVGRPQ AQMCCGQHLGTFLL*WSIASYHICMRNLKRVPG VKQDR*NTPYL*TERH*KGRLVGTVAPALLLVQV QRGMLTSALSAAITHRDITMESGRVKDVRPFLK EAFKDIMIIFVQLQISVQSIKTGARAARPADFG SVTKWEW*SVAPGERDVGTALCGDREVPTSSCTV PARPREVAATRPECGSCCWTP*APSS*CSPSWR LSRPMC*SAAPVRPSPRPP**CP*PSWPTRSWY T*SAGPRRFPALWSSACSTKCGSWRAVGWRC**W G*CGAQLTTPASSSLLQILFWTGMRGNA*KEFW KSLTCSWQLLQGFES*NSNTKNISVSRP*SCSI PVCTLWSQRPRMLTAAGSWLTC*TP*PMLWFG*L PRAASPPSSNPCAWLTS*CSCPTSGMRGQKRPL KHSPHLE*RWRLFCLKQRWSSDPLINSVA*RKI LGNIFTSVSLWDHCNP*KK*F*RKS*NTLKLVMW LKTTFLSIINPTI**L*TLK*RRILFRWSP*LP RACRCLTHEGHQCRKLRLHLLMSFTGFP*GLCF GREGEEGTGIVWSAVPANRCRLGTVFSIFQ*ERG NADAYPLCLGRK*NATGLKWATRGFACSFAMER AGNPGAVAHTCNPNTLGGRGGQIS*GQEFETSL ANMAKPRLY*KYNN*PGMVVCACNPSYSGG*GMR MA*TWKAKVAVSRDWATALQPG*LTE*DSVK

Sequence ESR2.cAug05

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

Frame 1

Frame 2

Sequence ESR2.bAug05

CTCGGTCTTTAAAAGGAAGAAGGGGCTTATCGTTAAGTCGCTTGTGATCTTTTCAGTTTCTCCAGCTGCTGGCTTTTTGGACACCCACTCCCCCGCCAGG AGGCAGTTGCAAGCGCGGAGGCTGCGAGAAATAACTGCCTCTTGAAACTTGCAGGGCGAAGAGCAGGCGGCGAGCGCTGGGCCGGGGAGGGACCACCCGA GCTGCGACGGGCTCTGGGGCTGCGGGGCAGGGCTGGCGCCCGGAGCCTGAGCTGCAGGAGGTGCGCTCGCTTTCCTCAACAGGTGGCGGCGGGGCGCGCG CCGGGAGACCCCCCCTAATGCGGGAAAAGCACGTGTCCGCATTTTAGAGAAGGCAAGGCCGGTGTGTTTATCTGCAAGCCATTATACTTGCCCACGAATC TTTGAGAACATTATAATGACCTTTGTGCCTCTTCTTGCAAGGTGTTTTCTCAGCTGTTATCTCAAGACATGGATATAAAAAACTCACCATCTAGCCTTAA TTCTCCTTCCTCCTACAACTGCAGTCAATCCATCTTACCCCTGGAGCACGGCTCCATATACATACCTTCCTCCTATGTAGACAGCCACCATGAATATCCA GCCATGACATTCTATAGCCCTGCTGTGATGAATTACAGCATTCCCAGCAATGTCACTAACTTGGAAGGTGGGCCTGGTCGGCAGACCACAAGCCCAAATG TGTTGTGGCCAACACCTGGGCACCTTTCTCCTTTAGTGGTCCATCGCCAGTTATCACATCTGTATGCGGAACCTCAAAAGAGTCCCTGGTGTGAAGCAAG ATCGCTAGAACACACCTTACCTGTAAACAGAGAGACACTGAAAAGGAAGGTTAGTGGGAACCGTTGCGCCAGCCCTGTTACTGGTCCAGGTTCAAAGAGG GATGCTCACTTCTGCGCTGTCTGCAGCGATTACGCATCGGGATATCACTATGGAGTCTGGTCGTGTGAAGGATGTAAGGCCTTTTTTAAAAGAAGCATTC AAGGACATAATGATTATATTTGTCCAGCTACAAATCAGTGTACAATCGATAAAAACCGGCGCAAGAGCTGCCAGGCCTGCCGACTTCGGAAGTGTTACGA AGTGGGAATGGTGAAGTGTGGCTCCCGGAGAGAGAGATGTGGGTACCGCCTTGTGCGGAGACAGAGAAGTGCCGACGAGCAGCTGCACTGTGCCGGCAAG GCCAAGAGAAGTGGCGGCCACGCGCCCCGAGTGCGGGAGCTGCTGCTGGACGCCCTGAGCCCCGAGCAGCTAGTGCTCACCCTCCTGGAGGCTGAGCCGC CCCATGTGCTGATCAGCCGCCCCAGTGCGCCCTTCACCGAGGCCTCCATGATGATGTCCCTGACCAAGTTGGCCGACAAGGAGTTGGTACACATGATCAG CTGGGCCAAGAAGATTCCCGGCTTTGTGGAGCTCAGCCTGTTCGACCAAGTGCGGCTCTTGGAGAGCTGTTGGATGGAGGTGTTAATGATGGGGCTGATG TGGCGCTCAATTGACCACCCCGGCAAGCTCATCTTTGCTCCAGATCTTGTTCTGGACAGGGATGAGGGGAAATGCGTAGAAGGAATTCTGGAAATCTTTG ACATGCTCCTGGCAACTACTTCAAGGTTTCGAGAGTTAAAACTCCAACACAAAGAATATCTCTGTGTCAAGGCCATGATCCTGCTCAATTCCAGTATGTA CCCTCTGGTCACAGCGACCCAGGATGCTGACAGCAGCCGGAAGCTGGCTCACTTGCTGAACGCCGTGACCGATGCTTTGGTTTGGGTGATTGCCAAGAGC GGCATCTCCTCCCAGCAGCAATCCATGCGCCTGGCTAACCTCCTGATGCTCCTGTCCCACGTCAGGCATGCGAGTAACAAGGGCATGGAACATCTGCTCA ACATGAAGTGCAAAAATGTGGTCCCAGTGTATGACCTGCTGCTGGAGATGCTGAATGCCCACGTGCTTCGCGGGTGCAAGTCCTCCATCACGGGGTCCGA GTGCAGCCCGGCAGAGGACAGTAAAAGCAAAGAGGGCTCCCAGAACCCACAGTCTCAGTGACGCCTGGCCCTGAGGTGAACTGGCCCACAGAGGTCACAG GCTGAAGCGTGAACTCCAGTGTGTCAGGAGCCTGGGCTTCATCTTTCTGCTGTGTGGTCCCTCATTTGGTGATGGCAGGCTTGGTCATGTACCATCCTTC CCTCCACCTTCCCAACTCTCAGGAGTCGGTGTGAGGAAGCCATAGTTTCCCTTGTTAGCAGAGGGCACATTTGAATGCAGCGTTTCCACACTCAATGGCC TCATAGGATCTCAGTGTGGTCTTTCTTACTTTCCTTCTTCCTTCCTCCCCTTTGTGAAACATCTTAAAGGTTTTGGAATGAATGGTGGAAATCTGACTTG GAAGGGCTGCGAATCAGAAAGGGGAGAGGAAGTGACACGCTTACAGAAGTGGGCTAACCCTTCTTGTGTGGCACACACTACCCTTCCCTCTGAGAGTTGA CCTTTGCTGTTTTCCGGACCACTCCATTGTAAGATTGAAAACCCCTGTGGCAATTGCGTACTTACCTCCCAGGCCTGTGGGGACTGATCATATCATATGA TGCTTATTCTGTCAAAGGCCAGAGGGACTGTGGTTAAGCTGGGATGTGAGTCATGTTCTCTCCCTGACCTTGCTGCCAGCTGCACACAGATTTGTCCCTC TCGATTTGTATTCACAGAGCCTGCCAATAATTTGGGGTATGTGTGTATGAGCGTGTGATCATTTTCATGCAGGACTGTGGGAGATACAAATCTCGCTGCT TCTGGAGCTGCTCTTCCTTAAACCTGTTGTCCCATGGGGCCAGCGTGGGTGCTGGAGAAAGGCCGTGTTTGCAGGAATGGGGTTCTCTCCTGTGGGTGTG GGTGACAGCCACAGTGTTTCCCTGGGGCAATGTGGATGCAGTTTCCATCTTGTACAACCTCATAAGTAGCAGCCACAATTGCCCCATCAGTCACCACAAG TAGTCAGGGATACTTTGGGCTGTGGATGTGTGCAGTGTGCTGTTTTATGGATGGATGAGTAGCTATGCACCCCAGTGTGTCAGCTCTGGGGCCACACTGT ATAGCCTTGATGAGTACGCCCCTTGAACAAGACCCAGTTTGTGAACTCTCCTTAAAGAGAAATATTTAGGGATAATTATTTATAGCAAGAAAGAATTCTT TTACACTTGAGAGCTCTTTTAAAAATATTTTCTTATTGGAAAATTTATATGGTGGGCAGGGTGAAAAAGAAACAGTAAAAATATTAGTTCTTATTCCAAG TGGAACATAAATAGGACATGAAGAAGGGCACCTCTGAAATGACAACTTTAACTCACCTTTTAAAAGATGTGAAATTTCCAGTTTTGGATACACGGTGAAT ATGTAAAATGAGTAACAGCATACTATGGAAGCCAGCAATTAAATAATCATGTTTCATTATTGCAGTAACGTTTTAAACAATTACCTTGTGATATGATATT AAATATATTTTCTTTTTGAAAATATGTTCACTTTGGGTAGCACATCCTGTATTTACTAAGTCATTAGGAAGACTGCATTCAGTGTTACCAAGACTGGTTT TTGCTAGTAAGACCTCGAATAATCCATAATTTTGATATTGGTGCAATTTTACTATAAGTTGAGCTTAGCTGTTTCAGAAATGCTTGGACAAGTACCTAGA GAACACACTGATGTCTGTGTTCTGAGGCAGTCTGAAGTTATTCTTAGAGACTCAGTTACAGCTTTAGTAAGATTTAGTACAGGCAGGATAAGCTTGGTTT CATAGGAACCAGGGAACCAGTGTTAGTGTCAGCTTCTTTCCTCCTGGTCAGCCTAGAATCCCCCACTCCCAATAGAGGGGTTTGGAAGCTGGAGAGTAGG AAGTAAGAGGCAAAGAAGGCAGCCTTCAGCAACTCATTATCTGCCAGTGAAATTCTATTAAATGTATTTTTAAAAGAGATTACCAGGTAACAAAAACATA AAAAACCAAAACAAGGCCAGATGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGAACCACTTGAGCCCATGAGTTTGACTCC AGGCTGGGCAACATGGAAACCCTGTCCTACAAAAGATACAAAAATTAGCCAGGCGTGGTGGTGCAGGCCTGTAGTTCCAGCTACCTGGGAGGCTGAGGTG GGAGGATCACCTGAGCCTGGGGAGATCAAGGCTGCAGTCCATTGCACTCCAGCCTGGGTGACAGAGGGAGACCCTGTCTC

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

LGL*KEEGAYR*VACDLFSFSSCWLFGHPLPRQ EAVASAEAARNNCLLKLAGRRAGGERWAGEGPPE LRRALGLRGRAGARSLSCRRCARFPQQVAAGRA PGDPP*CGKSTCPHFREGKAGVFICKPLYLPTN L*EHYNDLCASSCKVFSQLLSQDMDIKNSPSSLN SPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYP AMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPN VLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEAR SLEHTLPVNRETLKRKVSGNRCASPVTGPGSKR DAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSI QGHNDYICPATNQCTIDKNRRKSCQACRLRKCYE VGMVKCGSRRERCGYRLVRRQRSADEQLHCAGK AKRSGGHAPRVRELLLDALSPEQLVLTLLEAEP PHVLISRPSAPFTEASMMMSLTKLADKELVHMIS WAKKIPGFVELSLFDQVRLLESCWMEVLMMGLM WRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIF DMLLATTSRFRELKLQHKEYLCVKAMILLNSSMY PLVTATQDADSSRKLAHLLNAVTDALVWVIAKS GISSQQQSMRLANLLMLLSHVRHASNKGMEHLL NMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSE CSPAEDSKSKEGSQNPQSQ*RLALR*TGPQRSQ AEA*TPVCQEPGLHLSAVWSLIW*WQAWSCTIL PSTFPTLRSRCEEAIVSLVSRGHI*MQRFHTQWP HRISVWSFLLSFFLPPLCETS*RFWNEWWKSDL EGLRIRKGRGSDTLTEVG*PFLCGTHYPSL*EL TFAVFRTTPL*D*KPLWQLRTYLPGLWGLIISYD AYSVKGQRDCG*AGM*VMFSP*PCCQLHTDLSL SICIHRACQ*FGVCVYERVIIFMQDCGRYKSRC FWSCSSLNLLSHGASVGAGERPCLQEWGSLLWVW VTATVFPWGNVDAVSILYNLISSSHNCPISHHK *SGILWAVDVCSVLFYGWMSSYAPQCVSSGATL YSLDEYAP*TRPSL*TLLKEKYLGIIIYSKKEFF YT*ELF*KYFLIGKFIWWAG*KRNSKNISSYSK WNINRT*RRAPLK*QL*LTF*KM*NFQFWIHGE YVK*VTAYYGSQQLNNHVSLLQ*RFKQLPCDMIL NIFSF*KYVHFG*HILYLLSH*EDCIQCYQDWF LLVRPRIIHNFDIGAILL*VELSCFRNAWTST* RTH*CLCSEAV*SYS*RLSYSFSKI*YRQDKLGF IGTREPVLVSASFLLVSLESPTPNRGVWKLESR K*EAKKAAFSNSLSASEILLNVFLKEITR*QKH KKPKQGQMWWLTPVIPALWEAEVGEPLEPMSLTP GWATWKPCPTKDTKISQAWWCRPVVPATWEAEV GGSPEPGEIKAAVHCTPAWVTEGDPV

Frame 1

SVFKRKKGLIVKSLVIFSVSPAAGFLDTHSPAR RQLQARRLREITAS*NLQGEEQAASAGPGRDHP SCDGLWGCGAGLAPGA*AAGGALAFLNRWRRGAR RETPPNAGKARVRILEKARPVCLSASHYTCPRI FENIIMTFVPLLARCFLSCYLKTWI*KTHHLAL ILLPPTTAVNPSYPWSTAPYTYLPPM*TATMNIQ P*HSIALL**ITAFPAMSLTWKVGLVGRPQAQM CCGQHLGTFLL*WSIASYHICMRNLKRVPGVKQ DR*NTPYL*TERH*KGRLVGTVAPALLLVQVQRG MLTSALSAAITHRDITMESGRVKDVRPFLKEAF KDIMIIFVQLQISVQSIKTGARAARPADFGSVT KWEW*SVAPGERDVGTALCGDREVPTSSCTVPAR PREVAATRPECGSCCWTP*APSS*CSPSWRLSR PMC*SAAPVRPSPRPP**CP*PSWPTRSWYT*S AGPRRFPALWSSACSTKCGSWRAVGWRC**WG*C GAQLTTPASSSLLQILFWTGMRGNA*KEFWKSL TCSWQLLQGFES*NSNTKNISVSRP*SCSIPVC TLWSQRPRMLTAAGSWLTC*TP*PMLWFG*LPRA ASPPSSNPCAWLTS*CSCPTSGMRVTRAWNICS T*SAKMWSQCMTCCWRC*MPTCFAGASPPSRGP SAARQRTVKAKRAPRTHSLSDAWP*GELAHRGHR LKRELQCVRSLGFIFLLCGPSFGDGRLGHVPSF PPPSQLSGVGVRKP*FPLLAEGTFECSVSTLNG LIGSQCGLSYFPSSFLPFVKHLKGFGMNGGNLTW KGCESERGEEVTRLQKWANPSCVAHTTLPSES* PLLFSGPLHCKIENPCGNCVLTSQACGD*SYHM MLILSKARGTVVKLGCESCSLPDLAASCTQICPS RFVFTEPANNLGYVCMSV*SFSCRTVGDTNLAA SGAALP*TCCPMGPAWVLEKGRVCRNGVLSCGC G*QPQCFPGAMWMQFPSCTTS*VAATIAPSVTTS SQGYFGLWMCAVCCFMDG*VAMHPSVSALGPHC IALMSTPLEQDPVCELSLKRNI*G*LFIARKNS FTLESSFKNIFLLENLYGGQGEKETVKILVLIPS GT*IGHEEGHL*NDNFNSPFKRCEISSFGYTVN M*NE*QHTMEASN*IIMFHYCSNVLNNYLVI*Y *IYFLFENMFTLGSTSCIY*VIRKTAFSVTKTGF C**DLE*SIILILVQFYYKLSLAVSEMLGQVPR EHTDVCVLRQSEVILRDSVTALVRFSTGRISLV S*EPGNQC*CQLLSSWSA*NPPLPIEGFGSWRVG SKRQRRQPSATHYLPVKFY*MYF*KRLPGNKNI KNQNKARCGGSRL*SQHFGRPRWANHLSP*V*L QAGQHGNPVLQKIQKLARRGGAGL*FQLPGRLRW EDHLSLGRSRLQSIALQPG*QRETLS

Frame 2

RSLKGRRGLSLSRL*SFQFLQLLAFWTPTPPPG GSCKRGGCEK*LPLETCRAKSRRRALGRGGTTR AATGSGAAGQGWRPEPELQEVRSLSSTGGGGAR AGRPPLMREKHVSAF*RRQGRCVYLQAIILAHES LRTL**PLCLFLQGVFSAVISRHGYKKLTI*P* FSFLLQLQSIHLTPGARLHIHTFLLCRQPP*IS SHDIL*PCCDELQHSQQCH*LGRWAWSADHKPKC VVANTWAPFSFSGPSPVITSVCGTSKESLV*SK IARTHLTCKQRDTEKEG*WEPLRQPCYWSRFKE GCSLLRCLQRLRIGISLWSLVV*RM*GLF*KKHS RT**LYLSSYKSVYNR*KPAQELPGLPTSEVLR SGNGEVWLPEREMWVPPCAETEKCRRAAALCRQ GQEKWRPRAPSAGAAAGRPEPRAASAHPPGG*AA PCADQPPQCALHRGLHDDVPDQVGRQGVGTHDQ LGQEDSRLCGAQPVRPSAALGELLDGGVNDGAD VALN*PPRQAHLCSRSCSGQG*GEMRRRNSGNL* HAPGNYFKVSRVKTPTQRISLCQGHDPAQFQYV PSGHSDPGC*QQPEAGSLAERRDRCFGLGDCQE RHLLPAAIHAPG*PPDAPVPRQACE*QGHGTSAQ HEVQKCGPSV*PAAGDAECPRASRVQVLHHGVR VQPGRGQ*KQRGLPEPTVSVTPGPEVNWPTEVT G*SVNSSVSGAWASSFCCVVPHLVMAGLVMYHPS LHLPNSQESV*GSHSFPC*QRAHLNAAFPHSMA S*DLSVVFLTFLLPSSPL*NILKVLE*MVEI*L GRAANQKGERK*HAYRSGLTLLVWHTLPFPLRVD LCCFPDHSIVRLKTPVAIAYLPPRPVGTDHII* CLFCQRPEGLWLSWDVSHVLSLTLLPAAHRFVP LDLYSQSLPIIWGMCV*ACDHFHAGLWEIQISLL LELLFLKPVVPWGQRGCWRKAVFAGMGFSPVGV GDSHSVSLGQCGCSFHLVQPHK*QPQLPHQSPQ VVRDTLGCGCVQCAVLWMDE*LCTPVCQLWGHTV *P**VRPLNKTQFVNSP*REIFRDNYL*QERIL LHLRALLKIFSYWKIYMVGRVKKKQ*KY*FLFQ VEHK*DMKKGTSEMTTLTHLLKDVKFPVLDTR*I CKMSNSILWKPAIK*SCFIIAVTF*TITL*YDI KYIFFLKICSLWVAHPVFTKSLGRLHSVLPRLV FASKTSNNP*F*YWCNFTIS*A*LFQKCLDKYLE NTLMSVF*GSLKLFLETQLQL**DLVQAG*AWF HRNQGTSVSVSFFPPGQPRIPHSQ*RGLEAGE* EVRGKEGSLQQLIICQ*NSIKCIFKRDYQVTKT* KTKTRPDVVAHACNPSTLGGRGGRTT*AHEFDS RLGNMETLSYKRYKN*PGVVVQACSSSYLGG*G GRIT*AWGDQGCSPLHSSLGDRGRPCL

Sequence ESR2.aAug05

GTTGACAGCCATTATACTTGCCCACGAATCTTTGAGAACATTATAATGACCTTTGTGCCTCTTCTTGCAAGGTGTTTTCTCAGCTGTTATCTCAAGACAT GGATATAAAAAACTCACCATCTAGCCTTAATTCTCCTTCCTCCTACAACTGCAGTCAATCCATCTTACCCCTGGAGCACGGCTCCATATACATACCTTCC TCCTATGTAGACAGCCACCATGAATATCCAGCCATGACATTCTATAGCCCTGCTGTGATGAATTACAGCATTCCCAGCAATGTCACTAACTTGGAAGGTG GGCCTGGTCGGCAGACCACAAGCCCAAATGTGTTGTGGCCAACACCTGGGCACCTTTCTCCTTTAGTGGTCCATCGCCAGTTATCACATCTGTATGCGGA ACCTCAAAAGAGTCCCTGGTGTGAAGCAAGATCGCTAGAACACACCTTACCTGTAAACAGAGAGACACTGAAAAGGAAGGTTAGTGGGAACCGTTGCGCC AGCCCTGTTACTGGTCCAGGTTCAAAGAGGGATGCTCACTTCTGCGCTGTCTGCAGCGATTACGCATCGGGATATCACTATGGAGTCTGGTCGTGTGAAG GATGTAAGGCCTTTTTTAAAAGAAGCATTCAAGGACATAATGATTATATTTGTCCAGCTACAAATCAGTGTACAATCGATAAAAACCGGCGCAAGAGCTG CCAGGCCTGCCGACTTCGGAAGTGTTACGAAGTGGGAATGGTGAAGTGTGGCTCCCGGAGAGAGAGATGTGGGTACCGCCTTGTGCGGAGACAGAGAAGT GCCGACGAGCAGCTGCACTGTGCCGGCAAGGCCAAGAGAAGTGGCGGCCACGCGCCCCGAGTGCGGGAGCTGCTGCTGGACGCCCTGAGCCCCGAGCAGC TAGTGCTCACCCTCCTGGAGGCTGAGCCGCCCCATGTGCTGATCAGCCGCCCCAGTGCGCCCTTCACCGAGGCCTCCATGATGATGTCCCTGACCAAGTT GGCCGACAAGGAGTTGGTACACATGATCAGCTGGGCCAAGAAGATTCCCGGCTTTGTGGAGCTCAGCCTGTTCGACCAAGTGCGGCTCTTGGAGAGCTGT TGGATGGAGGTGTTAATGATGGGGCTGATGTGGCGCTCAATTGACCACCCCGGCAAGCTCATCTTTGCTCCAGATCTTGTTCTGGACAGGGATGAGGGGA AATGCGTAGAAGGAATTCTGGAAATCTTTGACATGCTCCTGGCAACTACTTCAAGGTTTCGAGAGTTAAAACTCCAACACAAAGAATATCTCTGTGTCAA GGCCATGATCCTGCTCAATTCCAGTATGTACCCTCTGGTCACAGCGACCCAGGATGCTGACAGCAGCCGGAAGCTGGCTCACTTGCTGAACGCCGTGACC GATGCTTTGGTTTGGGTGATTGCCAAGAGCGGCATCTCCTCCCAGCAGCAATCCATGCGCCTGGCTAACCTCCTGATGCTCCTGTCCCACGTCAGGCATG CGAGTAACAAGGGCATGGAACATCTGCTCAACATGAAGTGCAAAAATGTGGTCCCAGTGTATGACCTGCTGCTGGAGATGCTGAATGCCCACGTGCTTCG CGGGTGCAAGTCCTCCATCACGGGGTCCGAGTGCAGCCCGGCAGAGGACAGTAAAAGCAAAGAGGGCTCCCAGAACCCACAGTCTCAGTGACGCCTGGCC CTGAGGTGAACTGGCCCACAGAGGTCACAGGCTGAAGCGTGAACTCCAGTGTGTCAGGAGCCTGGGCTTCATCTTTCTGCTGTGTGGTCCCTCATTTGGT GATGGCAGGCTTGGTCATGTACCATCCTTCCCTCCACCTTCCCAACTCTCAGGAGTCGGTGTGAGGAAGCCATAGTTTCCCTTGTTAGCAGAGGGCACAT TTGAATGCAGCGTTTCCACACTCAATGGCCTCATAGGATCTCAGTGTGGTCTTTCTTACTTTCCTTCTTCCTTCCTCCCCTTTGTGAAACATCTTAAAGG TTTTGGAATGAATGGTGGAAATCTGACTTGGAAGGGCTGCGAATCAGAAAGGGGAGAGGAAGTGACACGCTTACAGAAGTGGGCTAACCCTTCTTGTGTG GCACACACTACCCTTCCCTCTGAGAGTTGACCTTTGCTGTTTTCCGGACCACTCCATTGTAAGATTGAAAACCCCTGTGGCAATTGCGTACTTACCTCCC AGGCCTGTGGGGACTGATCATATCATATGATGCTTATTCTGTCAAAGGCCAGAGGGACTGTGGTTAAGCTGGGATGTGAGTCATGTTCTCTCCCTGACCT TGCTGCCAGCTGCACACAGATTTGTCCCTCTCGATTTGTATTCACAGAGCCTGCCAATAATTTGGGGTATGTGTGTATGAGCGTGTGATCATTTTCATGC AGGACTGTGGGAGATACAAATCTCGCTGCTTCTGGAGCTGCTCTTCCTTAAACCTGTTGTCCCATGGGGCCAGCGTGGGTGCTGGAGAAAGGCCGTGTTT GCAGGAATGGGGTTCTCTCCTGTGGGTGTGGGTGACAGCCACAGTGTTTCCCTGGGGCAATGTGGATGCAGTTTCCATCTTGTACAACCTCATAAGTAGC AGCCACAATTGCCCCATCAGTCACCACAAGTAGTCAGGGATACTTTGGGCTGTGGATGTGTGCAGTGTGCTGTTTTATGGATGGATGAGTAGCTATGCAC CCCAGTGTGTCAGCTCTGGGGCCACACTGTATAGCCTTGATGAGTACGCCCCTTGAACAAGACCCAGTTTGTGAACTCTCCTTAAAGAGAAATATTTAGG GATAATTATTTATAGCAAGAAAGAATTCTTTTACACTTGAGAGCTCTTTTAAAAATATTTTCTTATTGGAAAATTTATATGGTGGGCAGGGTGAAAAAGA AACAGTAAAAATATTAGTTCTTATTCCAAGTGGAACATAAATAGGACATGAAGAAGGGCACCTCTGAAATGACAACTTTAACTCACCTTTTAAAAGATGT GAAATTTCCAGTTTTGGATACACGGTGAATATGTAAAATGAGTAACAGCATACTATGGAAGCCAGCAATTAAATAATCATGTTTCATTATTGCAGTAACG TTTTAAACAATTACCTTGTGATATGATATTAAATATATTTTCTTTTTGAAAATATGTTCACTTTGGGTAGCACATCCTGTATTTACTAAGTCATTAGGAA GACTGCATTCAGTGTTACCAAGACTGGTTTTTGCTAGTAAGACCTCGAATAATCCATAATTTTGATATTGGTGCAATTTTACTATAAGTTGAGCTTAGCT GTTTCAGAAATGCTTGGACAAGTACCTAGAGAACACACTGATGTCTGTGTTCTGAGGCAGTCTGAAGTTATTCTTAGAGACTCAGTTACAGCTTTAGTAA GATTTAGTACAGGCAGGATAAGCTTGGTTTCATAGGAACCAGGGAACCAGTGTTAGTGTCAGCTTCTTTCCTCCTGGTCAGCCTAGAATCCCCCACTCCC AATAGAGGGGTTTGGAAGCTGGAGAGTAGGAAGTAAGAGGCAAAGAAGGCAGCCTTCAGCAACTCATTATCTGCCAGTGAAATTCTATTAAATGTATTTT TAAAAGAGATTACCAGGTAACAAAAACATAAAAAACCAAAACAAGGCCAGATGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGG CGAACCACTTGAGCCCATGAGTTTGACTCCAGGCTGGGCAACATGGAAACCCTGTCCTACAAAAGATACAAAAATTAGCCAGGCGTGGTGGTGCAGGCCT GTAGTTCCAGCTACCTGGGAGGCTGAGGTGGGAGGATCACCTGAGCCTGGGGAGATCAAGGCTGCAGTCCATTGCACTCCAGCCTGGGTGACAGAGGGAG ACCCTGTCTC

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

VDSHYTCPRIFENIIMTFVPLLARCFLSCYLKT WI*KTHHLALILLPPTTAVNPSYPWSTAPYTYLP PM*TATMNIQP*HSIALL**ITAFPAMSLTWKV GLVGRPQAQMCCGQHLGTFLL*WSIASYHICMR NLKRVPGVKQDR*NTPYL*TERH*KGRLVGTVAP ALLLVQVQRGMLTSALSAAITHRDITMESGRVK DVRPFLKEAFKDIMIIFVQLQISVQSIKTGARA ARPADFGSVTKWEW*SVAPGERDVGTALCGDREV PTSSCTVPARPREVAATRPECGSCCWTP*APSS *CSPSWRLSRPMC*SAAPVRPSPRPP**CP*PS WPTRSWYT*SAGPRRFPALWSSACSTKCGSWRAV GWRC**WG*CGAQLTTPASSSLLQILFWTGMRG NA*KEFWKSLTCSWQLLQGFES*NSNTKNISVS RP*SCSIPVCTLWSQRPRMLTAAGSWLTC*TP*P MLWFG*LPRAASPPSSNPCAWLTS*CSCPTSGM RVTRAWNICST*SAKMWSQCMTCCWRC*MPTCF AGASPPSRGPSAARQRTVKAKRAPRTHSLSDAWP *GELAHRGHRLKRELQCVRSLGFIFLLCGPSFG DGRLGHVPSFPPPSQLSGVGVRKP*FPLLAEGT FECSVSTLNGLIGSQCGLSYFPSSFLPFVKHLKG FGMNGGNLTWKGCESERGEEVTRLQKWANPSCV AHTTLPSES*PLLFSGPLHCKIENPCGNCVLTS QACGD*SYHMMLILSKARGTVVKLGCESCSLPDL AASCTQICPSRFVFTEPANNLGYVCMSV*SFSC RTVGDTNLAASGAALP*TCCPMGPAWVLEKGRV CRNGVLSCGCG*QPQCFPGAMWMQFPSCTTS*VA ATIAPSVTTSSQGYFGLWMCAVCCFMDG*VAMH PSVSALGPHCIALMSTPLEQDPVCELSLKRNI* G*LFIARKNSFTLESSFKNIFLLENLYGGQGEKE TVKILVLIPSGT*IGHEEGHL*NDNFNSPFKRC EISSFGYTVNM*NE*QHTMEASN*IIMFHYCSN VLNNYLVI*Y*IYFLFENMFTLGSTSCIY*VIRK TAFSVTKTGFC**DLE*SIILILVQFYYKLSLA VSEMLGQVPREHTDVCVLRQSEVILRDSVTALV RFSTGRISLVS*EPGNQC*CQLLSSWSA*NPPLP IEGFGSWRVGSKRQRRQPSATHYLPVKFY*MYF *KRLPGNKNIKNQNKARCGGSRL*SQHFGRPRW ANHLSP*V*LQAGQHGNPVLQKIQKLARRGGAGL *FQLPGRLRWEDHLSLGRSRLQSIALQPG*QRE TLS

Frame 1

LTAIILAHESLRTL**PLCLFLQGVFSAVISRH GYKKLTI*P*FSFLLQLQSIHLTPGARLHIHTF LLCRQPP*ISSHDIL*PCCDELQHSQQCH*LGRW AWSADHKPKCVVANTWAPFSFSGPSPVITSVCG TSKESLV*SKIARTHLTCKQRDTEKEG*WEPLR QPCYWSRFKEGCSLLRCLQRLRIGISLWSLVV*R M*GLF*KKHSRT**LYLSSYKSVYNR*KPAQEL PGLPTSEVLRSGNGEVWLPEREMWVPPCAETEK CRRAAALCRQGQEKWRPRAPSAGAAAGRPEPRAA SAHPPGG*AAPCADQPPQCALHRGLHDDVPDQV GRQGVGTHDQLGQEDSRLCGAQPVRPSAALGEL LDGGVNDGADVALN*PPRQAHLCSRSCSGQG*GE MRRRNSGNL*HAPGNYFKVSRVKTPTQRISLCQ GHDPAQFQYVPSGHSDPGC*QQPEAGSLAERRD RCFGLGDCQERHLLPAAIHAPG*PPDAPVPRQAC E*QGHGTSAQHEVQKCGPSV*PAAGDAECPRAS RVQVLHHGVRVQPGRGQ*KQRGLPEPTVSVTPG PEVNWPTEVTG*SVNSSVSGAWASSFCCVVPHLV MAGLVMYHPSLHLPNSQESV*GSHSFPC*QRAH LNAAFPHSMAS*DLSVVFLTFLLPSSPL*NILK VLE*MVEI*LGRAANQKGERK*HAYRSGLTLLVW HTLPFPLRVDLCCFPDHSIVRLKTPVAIAYLPP RPVGTDHII*CLFCQRPEGLWLSWDVSHVLSLT LLPAAHRFVPLDLYSQSLPIIWGMCV*ACDHFHA GLWEIQISLLLELLFLKPVVPWGQRGCWRKAVF AGMGFSPVGVGDSHSVSLGQCGCSFHLVQPHK* QPQLPHQSPQVVRDTLGCGCVQCAVLWMDE*LCT PVCQLWGHTV*P**VRPLNKTQFVNSP*REIFR DNYL*QERILLHLRALLKIFSYWKIYMVGRVKK KQ*KY*FLFQVEHK*DMKKGTSEMTTLTHLLKDV KFPVLDTR*ICKMSNSILWKPAIK*SCFIIAVT F*TITL*YDIKYIFFLKICSLWVAHPVFTKSLG RLHSVLPRLVFASKTSNNP*F*YWCNFTIS*A*L FQKCLDKYLENTLMSVF*GSLKLFLETQLQL** DLVQAG*AWFHRNQGTSVSVSFFPPGQPRIPHS Q*RGLEAGE*EVRGKEGSLQQLIICQ*NSIKCIF KRDYQVTKT*KTKTRPDVVAHACNPSTLGGRGG RTT*AHEFDSRLGNMETLSYKRYKN*PGVVVQA CSSSYLGG*GGRIT*AWGDQGCSPLHSSLGDRGR PCL

Frame 2

*QPLYLPTNL*EHYNDLCASSCKVFSQLLSQDM DIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPS SYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEG GPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAE PQKSPWCEARSLEHTLPVNRETLKRKVSGNRCA SPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCE GCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSC QACRLRKCYEVGMVKCGSRRERCGYRLVRRQRS ADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQ LVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKL ADKELVHMISWAKKIPGFVELSLFDQVRLLESC WMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEG KCVEGILEIFDMLLATTSRFRELKLQHKEYLCVK AMILLNSSMYPLVTATQDADSSRKLAHLLNAVT DALVWVIAKSGISSQQQSMRLANLLMLLSHVRH ASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLR GCKSSITGSECSPAEDSKSKEGSQNPQSQ*RLA LR*TGPQRSQAEA*TPVCQEPGLHLSAVWSLIW *WQAWSCTILPSTFPTLRSRCEEAIVSLVSRGHI *MQRFHTQWPHRISVWSFLLSFFLPPLCETS*R FWNEWWKSDLEGLRIRKGRGSDTLTEVG*PFLC GTHYPSL*ELTFAVFRTTPL*D*KPLWQLRTYLP GLWGLIISYDAYSVKGQRDCG*AGM*VMFSP*P CCQLHTDLSLSICIHRACQ*FGVCVYERVIIFM QDCGRYKSRCFWSCSSLNLLSHGASVGAGERPCL QEWGSLLWVWVTATVFPWGNVDAVSILYNLISS SHNCPISHHK*SGILWAVDVCSVLFYGWMSSYA PQCVSSGATLYSLDEYAP*TRPSL*TLLKEKYLG IIIYSKKEFFYT*ELF*KYFLIGKFIWWAG*KR NSKNISSYSKWNINRT*RRAPLK*QL*LTF*KM *NFQFWIHGEYVK*VTAYYGSQQLNNHVSLLQ*R FKQLPCDMILNIFSF*KYVHFG*HILYLLSH*E DCIQCYQDWFLLVRPRIIHNFDIGAILL*VELS CFRNAWTST*RTH*CLCSEAV*SYS*RLSYSFSK I*YRQDKLGFIGTREPVLVSASFLLVSLESPTP NRGVWKLESRK*EAKKAAFSNSLSASEILLNVF LKEITR*QKHKKPKQGQMWWLTPVIPALWEAEVG EPLEPMSLTPGWATWKPCPTKDTKISQAWWCRP VVPATWEAEVGGSPEPGEIKAAVHCTPAWVTEG DPV

Sequence ESR2.iAug05

ESR2-F1 (forward primer)
ESR2-R1 (reverse primer)
Alternative acceptor

Frame 0

MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIP SSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEG GPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYA EPQKSPWCEARSLEHTLPVNRT**LYLSSYKSV YNR*KPAQELPGLPTSEVLRSGNGEVWLPEREMW VPPCAETEKCRRAAALCRQGQEKWRPRAPSAGA AAGRPEPRAASAHPPGG*AAPCADQPPQCALHR GLHDDVPDQVGRQGVGTHDQLGQEDSRELRRRGG GVSPPLAFPRLCGAQPVRPSAALGELLDGGVND GADVALN*PPRQAHLCSRSCSGQG*GEMRRRNS GNL*HAPGNYFKVSRVKTPTQRISLCQGHDPAQF QYVPSGHSDPGC*QQPEAGSLAERRDRCFGLGD CQERHLLPAAIHAPG*PPDAPVPRQACE*QGHG TSAQHEVQKCGPSV*PAAGDAECPRASRVQVLHH GVRVQPGRGQ*KQRGLPEPTVSVTPGPEVNWPT EVTG*SVNSSVSGAWASSFCCVVPHLVMAGLVM YHPSLHLPNSQESV*GSHSFPC*QRAHLNAAFPH SMAS*DLSVVFLTFLLPSSPL*NILKVLE*MVE I*LGRAANQKGERK*HAYRSGLTLLVWHTLPFP LRVDLCCFPDHSIVRLKTPVAIAYLPPRPVGTDH II*CLFCQRPEGLWLSWDVSHVLSLTLLPAAHR FVPLDLYSQSLPIIWGMCV*ACDHFHAGLWEIQ ISLLLELLFLKPVVPWGQRGCWRKAVFAGMGFSP VGVGDSHSVSLGQCGCSFHLVQPHK*QPQLPHQ SPQVVRDTLGCGCVQCAVLWMDE*LCTPVCQLW GHTV*P**VRPLNKTQFVNSP*REIFRDNYL*QE RILLHLRALLKIFSYWKIYMVGRVKKKQ*KY*F LFQVEHK*DMKKGTSEMTTLTHLLKDVKFPVLD TR*ICKMSNSILWKPAIK*SCFIIAVTF*TITL* YDIKYIFFLKICSLWVAHPVFTKSLGRLHSVLP RLVFASKTSNNP*F*YWCNFTIS*A*LFQKCLD KYLENTLMSVF*GSLKLFLETQLQL**DLVQAG* AWFHRNQGTSVSVSFFPPGQPRIPHSQ*RGLEA GE*EVRGKEGSLQQLIICQ*NSIKCIFKRDYQV TKT*KTKTRPDVVAHACNPSTLGGRGGRTT*AHE FDSRLGNMETLSYKRYKN*PGVVVQACSSSYLG G*GGRIT*AWGDQGCSPLHSSLGDRGRPCL

Frame 1

WI*KTHHLALILLPPTTAVNPSYPWSTAPYTYL PPM*TATMNIQP*HSIALL**ITAFPAMSLTWK VGLVGRPQAQMCCGQHLGTFLL*WSIASYHICMR NLKRVPGVKQDR*NTPYL*TGHNDYICPATNQC TIDKNRRKSCQACRLRKCYEVGMVKCGSRRERC GYRLVRRQRSADEQLHCAGKAKRSGGHAPRVREL LLDALSPEQLVLTLLEAEPPHVLISRPSAPFTE ASMMMSLTKLADKELVHMISWAKKIPGS*GGGV GVSHRLLLSPGFVELSLFDQVRLLESCWMEVLMM GLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGIL EIFDMLLATTSRFRELKLQHKEYLCVKAMILLN SSMYPLVTATQDADSSRKLAHLLNAVTDALVWVI AKSGISSQQQSMRLANLLMLLSHVRHASNKGME HLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSI TGSECSPAEDSKSKEGSQNPQSQ*RLALR*TGPQ RSQAEA*TPVCQEPGLHLSAVWSLIW*WQAWSC TILPSTFPTLRSRCEEAIVSLVSRGHI*MQRFH TQWPHRISVWSFLLSFFLPPLCETS*RFWNEWWK SDLEGLRIRKGRGSDTLTEVG*PFLCGTHYPSL *ELTFAVFRTTPL*D*KPLWQLRTYLPGLWGLI ISYDAYSVKGQRDCG*AGM*VMFSP*PCCQLHTD LSLSICIHRACQ*FGVCVYERVIIFMQDCGRYK SRCFWSCSSLNLLSHGASVGAGERPCLQEWGSL LWVWVTATVFPWGNVDAVSILYNLISSSHNCPIS HHK*SGILWAVDVCSVLFYGWMSSYAPQCVSSG ATLYSLDEYAP*TRPSL*TLLKEKYLGIIIYSK KEFFYT*ELF*KYFLIGKFIWWAG*KRNSKNISS YSKWNINRT*RRAPLK*QL*LTF*KM*NFQFWI HGEYVK*VTAYYGSQQLNNHVSLLQ*RFKQLPC DMILNIFSF*KYVHFG*HILYLLSH*EDCIQCYQ DWFLLVRPRIIHNFDIGAILL*VELSCFRNAWT ST*RTH*CLCSEAV*SYS*RLSYSFSKI*YRQD KLGFIGTREPVLVSASFLLVSLESPTPNRGVWKL ESRK*EAKKAAFSNSLSASEILLNVFLKEITR* QKHKKPKQGQMWWLTPVIPALWEAEVGEPLEPM SLTPGWATWKPCPTKDTKISQAWWCRPVVPATWE AEVGGSPEPGEIKAAVHCTPAWVTEGDPV

Frame 2

GYKKLTI*P*FSFLLQLQSIHLTPGARLHIHTF LLCRQPP*ISSHDIL*PCCDELQHSQQCH*LGR WAWSADHKPKCVVANTWAPFSFSGPSPVITSVC GTSKESLV*SKIARTHLTCKQDIMIIFVQLQISV QSIKTGARAARPADFGSVTKWEW*SVAPGERDV GTALCGDREVPTSSCTVPARPREVAATRPECGS CCWTP*APSS*CSPSWRLSRPMC*SAAPVRPSPR PP**CP*PSWPTRSWYT*SAGPRRFPGAEEEGW GCLTASCFPQALWSSACSTKCGSWRAVGWRC** WG*CGAQLTTPASSSLLQILFWTGMRGNA*KEFW KSLTCSWQLLQGFES*NSNTKNISVSRP*SCSI PVCTLWSQRPRMLTAAGSWLTC*TP*PMLWFG* LPRAASPPSSNPCAWLTS*CSCPTSGMRVTRAWN ICST*SAKMWSQCMTCCWRC*MPTCFAGASPPS RGPSAARQRTVKAKRAPRTHSLSDAWP*GELAH RGHRLKRELQCVRSLGFIFLLCGPSFGDGRLGHV PSFPPPSQLSGVGVRKP*FPLLAEGTFECSVST LNGLIGSQCGLSYFPSSFLPFVKHLKGFGMNGG NLTWKGCESERGEEVTRLQKWANPSCVAHTTLPS ES*PLLFSGPLHCKIENPCGNCVLTSQACGD*S YHMMLILSKARGTVVKLGCESCSLPDLAASCTQ ICPSRFVFTEPANNLGYVCMSV*SFSCRTVGDTN LAASGAALP*TCCPMGPAWVLEKGRVCRNGVLS CGCG*QPQCFPGAMWMQFPSCTTS*VAATIAPS VTTSSQGYFGLWMCAVCCFMDG*VAMHPSVSALG PHCIALMSTPLEQDPVCELSLKRNI*G*LFIAR KNSFTLESSFKNIFLLENLYGGQGEKETVKILV LIPSGT*IGHEEGHL*NDNFNSPFKRCEISSFGY TVNM*NE*QHTMEASN*IIMFHYCSNVLNNYLV I*Y*IYFLFENMFTLGSTSCIY*VIRKTAFSVT KTGFC**DLE*SIILILVQFYYKLSLAVSEMLGQ VPREHTDVCVLRQSEVILRDSVTALVRFSTGRI SLVS*EPGNQC*CQLLSSWSA*NPPLPIEGFGS WRVGSKRQRRQPSATHYLPVKFY*MYF*KRLPGN KNIKNQNKARCGGSRL*SQHFGRPRWANHLSP* V*LQAGQHGNPVLQKIQKLARRGGAGL*FQLPG RLRWEDHLSLGRSRLQSIALQPG*QRETLS