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Sequences

Sequence CASP6.eAug05

CCGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAGGAGGGCAAGGTGTCTGGCTGCGCGTTTGGCTGCAATGAGCTCGGCCTCGGGGCTCC GCAGGGGGCACCCGGCAGGTGGGGAAGAAAACATGACAGAAACAGATGCCTTCTATAAAAGAGAAATGTTTGATCCGGCAGAAAAGTACAAAATGGACCA CAGGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAGGTTCTTTTGGCACTTAACACTGCCAGAAAGGCGGGGCACCTGCGCAGATAGAGACAATCTT ACCCGCAGTGTCAACTGTTAGCCACGCAGATGCCGATTGCTTTGTGTGTGTCTTCCTGAGCCATGGCGAAGGCAATCACATTTATGCATATGATGCTAAA ATCGAAATTCAGACATTAACTGGCTTGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAAACCCAAGATATTTATCATTCAGGCATGTCGGGGAAACC AGCACGATGTGCCAGTCATTCCTTTGGATGTAGTAGATAATCAGACAGAGAAGTTGGACACCAACATAACTGAGGTGGATGCAGCCTCCGTTTACACGCT GCCTGCTGGAGCTGACTTCCTCATGTGTTACTCTGTTGCAGAAGGATATTATTCTCACCGGGAAACTGTGAACGGCTCATGGTACATTCAAGATTTGTGT GAGATGTTGGGAAAATATGGCTCCTCCTTAGAGTTCACAGAACTCCTCACACTGGTGAACAGGAAAGTTTCTCAGCGCCGAGTGGACTTTTGCAAAGACC CAAGTGCAATTGGAAAGAAGCAGGTTCCCTGTTTTGCCTCAATGCTAACTAAAAAGCTGCATTTCTTTCCAAAATCTAATTAATTAATAGAGGCTATCTA ATTTTACACTCTGTATTGAAAATGGCTTTCTCAGCCAGGCGTGGTTACTCACACCTGTAATCCCAGCACTTTGGGAGTCCAAGGTGGGCGGATCACCTGA GGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAGCCCCGTCTCTACTAAAAATGCAAAAAAAAATTTAGCTAGGCATGGCGGCGCATGCCTGCAA TCCCAGCTACTTGGAAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGCTGCGGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAA CGAGTGAAACTCCGTCTCAAAAAAAAGAAAATGTCTTTCTCTTCCTTTTATATAAATATCGTTAGGGTGAAGCATTATGGTCTAATGATTCAAATGTTTT AAAGTTTAATGCCTAGCAGAGAACTGCCTTAAAAAAAAAAAAAAAAAGTTCATGTTGGCCATGGTGAAAGGGTTTGATATGGAGAAACAAAATCCTCAGG AAATTAGATAAATAAAAATTTATAAGCATTTGTATTATTTTTTAATAAACTGCAGGGTTACACAAAAATCTAGCTGATTTAACTTGTATTTTGTCACTTT TTTATAAAAGTTTATTGTTTGATGTTTTTAAAGGTTTTTGAAATCCAGGAATTAAATCATCCCTTAATAAAATATTCGAAATTCATATTTTAT

casp6.f.2 (forward primer)
IFandCASP6andPLA2G12A.u.r.42 (reverse primer)
Single exon skipping

Frame 0

PRAGPGPGACGFRKRRARCLAARLAAMSSASGL RRGHPAGGEENMTETDAFYKREMFDPAEKYKMDH RRRGIALIFNHERFFWHLTLPERRGTCADRDNL TRSVNC*PRRCRLLCVCLPEPWRRQSHLCI*C* NRNSDINWLVQRRQVSQPGWKTQDIYHSGMSGKP ARCASHSFGCSR*SDREVGHQHN*GGCSLRLHA ACWS*LPHVLLCCRRILFSPGNCERLMVHSRFV *DVGKIWLLLRVHRTPHTGEQESFSAPSGLLQRP KCNWKEAGSLFCLNAN*KAAFLSKI*LINRGYL ILHSVLKMAFSARRGYSHL*SQHFGSPRWADHL RSGVRDQPDQHGEAPSLLKMQKKI*LGMAAHACN PSYLEG*GRRIT*TQEVEAAVSRDCAIALQPGQ RVKLRLKKKKMSFSSFYINIVRVKHYGLMIQMF *SLMPSRELP*KKKKKVHVGHGERV*YGETKSSG N*INKNL*AFVLFFNKLQGYTKI*LI*LVFCHF FIKVYCLMFLKVFEIQELNHPLIKYSKFIFY

Frame 1

RGRGRAREPVASGRGGQGVWLRVWLQ*ARPRGS AGGTRQVGKKT*QKQMPSIKEKCLIRQKSTKWT TGGEELL*SSIMRGSFGT*HCQKGGAPAQIETIL PAVSTVSHADADCFVCVFLSHGEGNHIYAYDAK IEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGN QHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTL PAGADFLMCYSVAEGYYSHRETVNGSWYIQDLC EMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKD PSAIGKKQVPCFASMLTKKLHFFPKSN*LIEAI* FYTLY*KWLSQPGVVTHTCNPSTLGVQGGRIT* GREFETSLTNMEKPRLY*KCKKKFS*AWRRMPA IPATWKAEAGESLEPRRWRLR*AEIAPLHSSLGN E*NSVSKKRKCLSLPFI*ISLG*SIMV**FKCF KV*CLAENCLKKKKKKFMLAMVKGFDMEKQNPQ EIR*IKIYKHLYYFLINCRVTQKSS*FNLYFVTF L*KFIV*CF*RFLKSRN*IIP**NIRNSYF

Frame 2

EGGAGPGSLWLQEEEGKVSGCAFGCNELGLGAP QGAPGRWGRKHDRNRCLL*KRNV*SGRKVQNGP QEERNCFNLQS*EVLLALNTARKAGHLRR*RQS YPQCQLLATQMPIALCVSS*AMAKAITFMHMMLK SKFRH*LACSKETSVTAWLENPRYLSFRHVGET STMCQSFLWM**IIRQRSWTPT*LRWMQPPFTR CLLELTSSCVTLLQKDIILTGKL*TAHGTFKICV RCWENMAPP*SSQNSSHW*TGKFLSAEWTFAKT QVQLERSRFPVLPQC*LKSCISFQNLIN**RLS NFTLCIENGFLSQAWLLTPVIPALWESKVGGSPE VGSSRPA*PTWRSPVSTKNAKKNLARHGGACLQ SQLLGRLRQENHLNPGGGGCGEPRLRHCTPAWA TSETPSQKKENVFLFLLYKYR*GEALWSNDSNVL KFNA*QRTALKKKKKSSCWPW*KGLIWRNKILR KLDK*KFISICIIF**TAGLHKNLADLTCILSL FYKSLLFDVFKGF*NPGIKSSLNKIFEIHIL

Sequence CASP6.fAug05

GGGCTTCAGCTGGAGGCGGCCAGTTCCTGGCGGCCAGCACACCCCGGTATCTCTCTTCTTCATCCAGGAAACCTGCTGCTGTCTGTCGGTTTGAGCCAAA ACCACAATAACTTTTTTCTGCAAAGTAATCAACTGCTAAGAGCGTGGGGAAGAAAACATGACAGAAACAGATGCCTTCTATAAAAGAGAAATGTTTGATC CGGCAGAAAAGTACAAAATGGACCACAGGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAGGTTCTTTTGGCACTTAACACTGCCAGAAAGGCGGGG CACCTGCGCAGATAGAGACAATCTTACCCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGCTTTAATGATCTTAAAGCAGAAGAACTACTGCTCAAA ATTCATGAGGTGTCAACTGTTAGCCACGCAGATGCCGATTGCTTTGTGTGTGTCTTCCTGAGCCATGGCGAAGGCAATCACATTTATGCATATGATGCTA AAATCGAAATTCAGACATTAACTGGCTTGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAAACCCAAGATATTTATCATTCAGGATATTATTCTCAC CGGGAAACTGTGAACGGCTCATGGTACATTCAAGATTTGTGTGAGATGTTGGGAAAATATGGCTCCTCCTTAGAGTTCACAGAACTCCTCACACTGGTGA ACAGGAAAGTTTCTCAGCGCCGAGTGGACTTTTGCAAAGACCCAAGTGCAATTGGAAAGAAGCAGGTTCCCTGTTTTGCCTCAATGCTAACTAAAAAGCT GCATTTCTTTCCAAAATCTAATTAATTAATAGAGGCTATCTAATTTTACACTCTGTATTGAAAATGGCTTTCTCAGCCAGGCGTGGTTACTCACACCTGT AATCCCAGCACTTTGGGAGTCCAAGGTGGGCGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAGCCCCGTCTCTACTAAAAAT GCAAAAAAAAATTTAGCTAGGCATGGCGGCGCATGCCTGCAATCCCAGCTACTTGGAAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGTGGAGGCT GCGGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAACGAGTGAAACTCCGTCTCAAAAAAAAGAAAATGTCTTTCTCTTCCTTTTATATAAATA TCGTTAGGGTGAAGCATTATGGTCTAATGATTCAAATGTTTTAAAGTTTAATGCCTAGCAGAGAACTGCCTTAAAAAAAAAAAAAAAAAGTTCATGTTGG CCATGGTGAAAGGGTTTGATATGGAGAAACAAAATCCTCAGGAAATTAGATAAATAAAAATTTATAAGCATTTGTATTATTTTTTAATAAACTGCAGGGT TACACAAAAATCTAGCTGATTTAACTTGTATTTTGTCACTTTTTTATAAAAGTTTATTGTTTGATGTTTTTAAAGGTTTTTGAAATCCAGGAATTAAATC ATCCCTTAATAAAATATTCGAAATTCATATTTTAT

casp6.f.2 (forward primer)
IFandCASP6andPLA2G12A.u.r.42 (reverse primer)
Single exon skipping

Frame 0

GLQLEAASSWRPAHPGISLLHPGNLLLSVGLSQ NHNNFFLQSNQLLRAWGRKHDRNRCLL*KRNV*S GRKVQNGPQEERNCFNLQS*EVLLALNTARKAG HLRR*RQSYPQVFRSRI*SEML**S*SRRTTAQ NS*GVNC*PRRCRLLCVCLPEPWRRQSHLCI*C* NRNSDINWLVQRRQVSQPGWKTQDIYHSGYYSH RETVNGSWYIQDLCEMLGKYGSSLEFTELLTLV NRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKL HFFPKSN*LIEAI*FYTLY*KWLSQPGVVTHTC NPSTLGVQGGRIT*GREFETSLTNMEKPRLY*K CKKKFS*AWRRMPAIPATWKAEAGESLEPRRWRL R*AEIAPLHSSLGNE*NSVSKKRKCLSLPFI*I SLG*SIMV**FKCFKV*CLAENCLKKKKKKFML AMVKGFDMEKQNPQEIR*IKIYKHLYYFLINCRV TQKSS*FNLYFVTFL*KFIV*CF*RFLKSRN*I IP**NIRNSYF

Frame 1

GFSWRRPVPGGQHTPVSLFFIQETCCCLSV*AK TTITFFCKVINC*ERGEENMTETDAFYKREMFD PAEKYKMDHRRRGIALIFNHERFFWHLTLPERRG TCADRDNLTRRFSDLGFEVKCFNDLKAEELLLK IHEVSTVSHADADCFVCVFLSHGEGNHIYAYDA KIEIQTLTGLFKGDKCHSLVGKPKIFIIQDIILT GKL*TAHGTFKICVRCWENMAPP*SSQNSSHW* TGKFLSAEWTFAKTQVQLERSRFPVLPQC*LKS CISFQNLIN**RLSNFTLCIENGFLSQAWLLTPV IPALWESKVGGSPEVGSSRPA*PTWRSPVSTKN AKKNLARHGGACLQSQLLGRLRQENHLNPGGGG CGEPRLRHCTPAWATSETPSQKKENVFLFLLYKY R*GEALWSNDSNVLKFNA*QRTALKKKKKSSCW PW*KGLIWRNKILRKLDK*KFISICIIF**TAG LHKNLADLTCILSLFYKSLLFDVFKGF*NPGIKS SLNKIFEIHIL

Frame 2

ASAGGGQFLAASTPRYLSSSSRKPAAVCRFEPK PQ*LFSAK*STAKSVGKKT*QKQMPSIKEKCLI RQKSTKWTTGGEELL*SSIMRGSFGT*HCQKGG APAQIETILPAGFQI*DLK*NALMILKQKNYCSK FMRCQLLATQMPIALCVSS*AMAKAITFMHMML KSKFRH*LACSKETSVTAWLENPRYLSFRILFS PGNCERLMVHSRFV*DVGKIWLLLRVHRTPHTGE QESFSAPSGLLQRPKCNWKEAGSLFCLNAN*KA AFLSKI*LINRGYLILHSVLKMAFSARRGYSHL *SQHFGSPRWADHLRSGVRDQPDQHGEAPSLLKM QKKI*LGMAAHACNPSYLEG*GRRIT*TQEVEA AVSRDCAIALQPGQRVKLRLKKKKMSFSSFYIN IVRVKHYGLMIQMF*SLMPSRELP*KKKKKVHVG HGERV*YGETKSSGN*INKNL*AFVLFFNKLQG YTKI*LI*LVFCHFFIKVYCLMFLKVFEIQELN HPLIKYSKFIFY

Sequence CASP6.dAug05

casp6.f.2 (forward primer)
IFandCASP6andPLA2G12A.u.r.42 (reverse primer)
Single exon skipping

Frame 0

PRAGPGPGACGFRKRRARCLAARLAAMSSASGL RRGHPAGGEENMTETDAFYKRKVQNGPQEERNCF NLQS*EVLLALNTARKAGHLRR*RQSYPQVFRS RI*SEML**S*SRRTTAQNS*GVNC*PRRCRLL CVCLPEPWRRQSHLCI*C*NRNSDINWLVQRRQV SQPGWKTQDIYHSGMSGKPARCASHSFGCSR*S DREVGHQHN*GGCSLRLHAACWS*LPHVLLCC

Frame 1

RGRGRAREPVASGRGGQGVWLRVWLQ*ARPRGS AGGTRQVGKKT*QKQMPSIKEKYKMDHRRRGIA LIFNHERFFWHLTLPERRGTCADRDNLTRRFSDL GFEVKCFNDLKAEELLLKIHEVSTVSHADADCF VCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDK CHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQ TEKLDTNITEVDAASVYTLPAGADFLMCYSV

Frame 2

EGGAGPGSLWLQEEEGKVSGCAFGCNELGLGAP QGAPGRWGRKHDRNRCLL*KKSTKWTTGGEELL *SSIMRGSFGT*HCQKGGAPAQIETILPAGFQI *DLK*NALMILKQKNYCSKFMRCQLLATQMPIAL CVSS*AMAKAITFMHMMLKSKFRH*LACSKETS VTAWLENPRYLSFRHVGETSTMCQSFLWM**II RQRSWTPT*LRWMQPPFTRCLLELTSSCVTLL

Sequence CASP6.bAug05

CCGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAGGAGGGCAAGGTGTCTGGCTGCGCGTTTGGCTGCAATGAGCTCGGCCTCGGGGCTCC GCAGGGGGCACCCGGCAGGTGGGGAAGAAAACATGACAGAAACAGATGCCTTCTATAAAAGAAATGTTTGATCCGGCAGAAAAGTACAAAATGGACCACA GGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAGGTTCTTTTGGCACTTAACACTGCCAGAAAGGCGGGGCACCTGCGCAGATAGAGACAATCTTAC CCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGCTTTAATGATCTTAAAGCAGAAGAACTACTGCTCAAAATTCATGAGGTGTCAACTGTTAGCCAC GCAGATGCCGATTGCTTTGTGTGTGTCTTCCTGAGCCATGGCGAAGGCAATCACATTTATGCATATGATGCTAAAATCGAAATTCAGACATTAACTGGCT TGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAAACCCAAGATATTTATCATTCAGGCATGTCGGGGAAACCAGCACGATGTGCCAGTCATTCCTTT GGATGTAGTAGATAATCAGACAGAGAAGTTGGACACCAACATAACTGAGGTGGATGCAGCCTCCGTTTACACGCTGCCTGCTGGAGCTGACTTCCTCATG TGTTACTCTGTTGCAGAAGGATATTATTCTCACCGGGAAACTGTGAACGGCTCATGGTACATTCAAGATTTGTGTGAGATGTTGGGAAAATATGGCTCCT CCTTAGAGTTCACAGAACTCCTCACACTGGTGAACAGGAAAGTTTCTCAGCGCCGAGTGGACTTTTGCAAAGACCCAAGTGCAATTGGAAAGAAGCAGGT TCCCTGTTTTGCCTCAATGCTAACTAAAAAGCTGCATTTCTTTCCAAAATCTAATTAATTAATAGAGGCTATCTAATTTTACACTCTGTATTGAAAATGG CTTTCTCAGCCAGGCGTGGTTACTCACACCTGTAATCCCAGCACTTTGGGAGTCCAAGGTGGGCGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGA CCAACATGGAGAAGCCCCGTCTCTACTAAAAATGCAAAAAAAAATTTAGCTAGGCATGGCGGCGCATGCCTGCAATCCCAGCTACTTGGAAGGCTGAGGC AGGAGAATCACTTGAACCCAGGAGGTGGAGGCTGCGGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAACGAGTGAAACTCCGTCTCAAAAAAA AGAAAATGTCTTTCTCTTCCTTTTATATAAATATCGTTAGGGTGAAGCATTATGGTCTAATGATTCAAATGTTTTAAAGTTTAATGCCTAGCAGAGAACT GCCTTAAAAAAAAAAAAAAAAAGTTCATGTTGGCCATGGTGAAAGGGTTTGATATGGAGAAACAAAATCCTCAGGAAATTAGATAAATAAAAATTTATAA GCATTTGTATTATTTTTTAATAAACTGCAGGGTTACACAAAAATCTAGCTGATTTAACTTGTATTTTGTCACTTTTTTATAAAAGTTTATTGTTTGATGT TTTTAAAGGTTTTTGAAATCCAGGAATTAAATCATCCCTTAATAAAATATTCGAAATTCATATTTTAT

casp6.f.2 (forward primer)
IFandCASP6andPLA2G12A.u.r.42 (reverse primer)
Single exon skipping

Frame 0

PRAGPGPGACGFRKRRARCLAARLAAMSSASGL RRGHPAGGEENMTETDAFYKRNV*SGRKVQNGPQ EERNCFNLQS*EVLLALNTARKAGHLRR*RQSY PQVFRSRI*SEML**S*SRRTTAQNS*GVNC*P RRCRLLCVCLPEPWRRQSHLCI*C*NRNSDINWL VQRRQVSQPGWKTQDIYHSGMSGKPARCASHSF GCSR*SDREVGHQHN*GGCSLRLHAACWS*LPH VLLCCRRILFSPGNCERLMVHSRFV*DVGKIWLL LRVHRTPHTGEQESFSAPSGLLQRPKCNWKEAG SLFCLNAN*KAAFLSKI*LINRGYLILHSVLKM AFSARRGYSHL*SQHFGSPRWADHLRSGVRDQPD QHGEAPSLLKMQKKI*LGMAAHACNPSYLEG*G RRIT*TQEVEAAVSRDCAIALQPGQRVKLRLKK KKMSFSSFYINIVRVKHYGLMIQMF*SLMPSREL P*KKKKKVHVGHGERV*YGETKSSGN*INKNL* AFVLFFNKLQGYTKI*LI*LVFCHFFIKVYCLM FLKVFEIQELNHPLIKYSKFIFY

Frame 1

RGRGRAREPVASGRGGQGVWLRVWLQ*ARPRGS AGGTRQVGKKT*QKQMPSIKEMFDPAEKYKMDH RRRGIALIFNHERFFWHLTLPERRGTCADRDNLT RRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSH ADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTG LFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPL DVVDNQTEKLDTNITEVDAASVYTLPAGADFLM CYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGS SLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQV PCFASMLTKKLHFFPKSN*LIEAI*FYTLY*KW LSQPGVVTHTCNPSTLGVQGGRIT*GREFETSL TNMEKPRLY*KCKKKFS*AWRRMPAIPATWKAEA GESLEPRRWRLR*AEIAPLHSSLGNE*NSVSKK RKCLSLPFI*ISLG*SIMV**FKCFKV*CLAEN CLKKKKKKFMLAMVKGFDMEKQNPQEIR*IKIYK HLYYFLINCRVTQKSS*FNLYFVTFL*KFIV*C F*RFLKSRN*IIP**NIRNSYF

Frame 2

EGGAGPGSLWLQEEEGKVSGCAFGCNELGLGAP QGAPGRWGRKHDRNRCLL*KKCLIRQKSTKWTT GGEELL*SSIMRGSFGT*HCQKGGAPAQIETIL PAGFQI*DLK*NALMILKQKNYCSKFMRCQLLAT QMPIALCVSS*AMAKAITFMHMMLKSKFRH*LA CSKETSVTAWLENPRYLSFRHVGETSTMCQSFL WM**IIRQRSWTPT*LRWMQPPFTRCLLELTSSC VTLLQKDIILTGKL*TAHGTFKICVRCWENMAP P*SSQNSSHW*TGKFLSAEWTFAKTQVQLERSR FPVLPQC*LKSCISFQNLIN**RLSNFTLCIENG FLSQAWLLTPVIPALWESKVGGSPEVGSSRPA* PTWRSPVSTKNAKKNLARHGGACLQSQLLGRLR QENHLNPGGGGCGEPRLRHCTPAWATSETPSQKK ENVFLFLLYKYR*GEALWSNDSNVLKFNA*QRT ALKKKKKSSCWPW*KGLIWRNKILRKLDK*KFI SICIIF**TAGLHKNLADLTCILSLFYKSLLFDV FKGF*NPGIKSSLNKIFEIHIL

Sequence CASP6.aAug05

CCGAGGGCGGGGCCGGGCCCGGGAGCCTGTGGCTTCAGGAAGAGGAGGGCAAGGTGTCTGGCTGCGCGTTTGGCTGCAATGAGCTCGGCCTCGGGGCTCC GCAGGGGGCACCCGGCAGGTGGGGAAGAAAACATGACAGAAACAGATGCCTTCTATAAAAGAGAAATGTTTGATCCGGCAGAAAAGTACAAAATGGACCA CAGGAGGAGAGGAATTGCTTTAATCTTCAATCATGAGAGGTTCTTTTGGCACTTAACACTGCCAGAAAGGCGGGGCACCTGCGCAGATAGAGACAATCTT ACCCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGCTTTAATGATCTTAAAGCAGAAGAACTACTGCTCAAAATTCATGAGGTGTCAACTGTTAGCC ACGCAGATGCCGATTGCTTTGTGTGTGTCTTCCTGAGCCATGGCGAAGGCAATCACATTTATGCATATGATGCTAAAATCGAAATTCAGACATTAACTGG CTTGTTCAAAGGAGACAAGTGTCACAGCCTGGTTGGAAAACCCAAGATATTTATCATTCAGGCATGTCGGGGAAACCAGCACGATGTGCCAGTCATTCCT TTGGATGTAGTAGATAATCAGACAGAGAAGTTGGACACCAACATAACTGAGGTGGATGCAGCCTCCGTTTACACGCTGCCTGCTGGAGCTGACTTCCTCA TGTGTTACTCTGTTGCAGAAGGATATTATTCTCACCGGGAAACTGTGAACGGCTCATGGTACATTCAAGATTTGTGTGAGATGTTGGGAAAATATGGCTC CTCCTTAGAGTTCACAGAACTCCTCACACTGGTGAACAGGAAAGTTTCTCAGCGCCGAGTGGACTTTTGCAAAGACCCAAGTGCAATTGGAAAGAAGCAG GTTCCCTGTTTTGCCTCAATGCTAACTAAAAAGCTGCATTTCTTTCCAAAATCTAATTAATTAATAGAGGCTATCTAATTTTACACTCTGTATTGAAAAT GGCTTTCTCAGCCAGGCGTGGTTACTCACACCTGTAATCCCAGCACTTTGGGAGTCCAAGGTGGGCGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCT GACCAACATGGAGAAGCCCCGTCTCTACTAAAAATGCAAAAAAAAATTTAGCTAGGCATGGCGGCGCATGCCTGCAATCCCAGCTACTTGGAAGGCTGAG GCAGGAGAATCACTTGAACCCAGGAGGTGGAGGCTGCGGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAACGAGTGAAACTCCGTCTCAAAAA AAAGAAAATGTCTTTCTCTTCCTTTTATATAAATATCGTTAGGGTGAAGCATTATGGTCTAATGATTCAAATGTTTTAAAGTTTAATGCCTAGCAGAGAA CTGCCTTAAAAAAAAAAAAAAAAAGTTCATGTTGGCCATGGTGAAAGGGTTTGATATGGAGAAACAAAATCCTCAGGAAATTAGATAAATAAAAATTTAT AAGCATTTGTATTATTTTTTAATAAACTGCAGGGTTACACAAAAATCTAGCTGATTTAACTTGTATTTTGTCACTTTTTTATAAAAGTTTATTGTTTGAT GTTTTTAAAGGTTTTTGAAATCCAGGAATTAAATCATCCCTTAATAAAATATTCGAAATTCATATTTTAT

casp6.f.2 (forward primer)
IFandCASP6andPLA2G12A.u.r.42 (reverse primer)
Single exon skipping

Frame 0

PRAGPGPGACGFRKRRARCLAARLAAMSSASGL RRGHPAGGEENMTETDAFYKREMFDPAEKYKMDH RRRGIALIFNHERFFWHLTLPERRGTCADRDNL TRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVS HADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTG LFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIP LDVVDNQTEKLDTNITEVDAASVYTLPAGADFL MCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGS SLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQ VPCFASMLTKKLHFFPKSN*LIEAI*FYTLY*K WLSQPGVVTHTCNPSTLGVQGGRIT*GREFETSL TNMEKPRLY*KCKKKFS*AWRRMPAIPATWKAE AGESLEPRRWRLR*AEIAPLHSSLGNE*NSVSK KRKCLSLPFI*ISLG*SIMV**FKCFKV*CLAEN CLKKKKKKFMLAMVKGFDMEKQNPQEIR*IKIY KHLYYFLINCRVTQKSS*FNLYFVTFL*KFIV* CF*RFLKSRN*IIP**NIRNSYF

Frame 1

RGRGRAREPVASGRGGQGVWLRVWLQ*ARPRGS AGGTRQVGKKT*QKQMPSIKEKCLIRQKSTKWT TGGEELL*SSIMRGSFGT*HCQKGGAPAQIETIL PAGFQI*DLK*NALMILKQKNYCSKFMRCQLLA TQMPIALCVSS*AMAKAITFMHMMLKSKFRH*L ACSKETSVTAWLENPRYLSFRHVGETSTMCQSFL WM**IIRQRSWTPT*LRWMQPPFTRCLLELTSS CVTLLQKDIILTGKL*TAHGTFKICVRCWENMA PP*SSQNSSHW*TGKFLSAEWTFAKTQVQLERSR FPVLPQC*LKSCISFQNLIN**RLSNFTLCIEN GFLSQAWLLTPVIPALWESKVGGSPEVGSSRPA *PTWRSPVSTKNAKKNLARHGGACLQSQLLGRLR QENHLNPGGGGCGEPRLRHCTPAWATSETPSQK KENVFLFLLYKYR*GEALWSNDSNVLKFNA*QR TALKKKKKSSCWPW*KGLIWRNKILRKLDK*KFI SICIIF**TAGLHKNLADLTCILSLFYKSLLFD VFKGF*NPGIKSSLNKIFEIHIL

Frame 2

EGGAGPGSLWLQEEEGKVSGCAFGCNELGLGAP QGAPGRWGRKHDRNRCLL*KRNV*SGRKVQNGP QEERNCFNLQS*EVLLALNTARKAGHLRR*RQS YPQVFRSRI*SEML**S*SRRTTAQNS*GVNC*P RRCRLLCVCLPEPWRRQSHLCI*C*NRNSDINW LVQRRQVSQPGWKTQDIYHSGMSGKPARCASHS FGCSR*SDREVGHQHN*GGCSLRLHAACWS*LPH VLLCCRRILFSPGNCERLMVHSRFV*DVGKIWL LLRVHRTPHTGEQESFSAPSGLLQRPKCNWKEA GSLFCLNAN*KAAFLSKI*LINRGYLILHSVLKM AFSARRGYSHL*SQHFGSPRWADHLRSGVRDQP DQHGEAPSLLKMQKKI*LGMAAHACNPSYLEG* GRRIT*TQEVEAAVSRDCAIALQPGQRVKLRLKK KKMSFSSFYINIVRVKHYGLMIQMF*SLMPSRE LP*KKKKKVHVGHGERV*YGETKSSGN*INKNL *AFVLFFNKLQGYTKI*LI*LVFCHFFIKVYCLM FLKVFEIQELNHPLIKYSKFIFY