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Sequences

Sequence CAPN3.pAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGAA
AAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACA
AGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAG
CAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGA
GTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAG
AAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGC
TCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC
TTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Alternative donor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVK
KKKTKPIIFVSDRANSNKELGVDQESEEGKGKT
SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQI
AGDDMEICADELKKVLNTVVNKHKDLKTHGFTLE
SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ
KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQ
LYDIITMRYADKHMNIDFDSFICCFVRLEGMFRA
FHAFDKDGDGIIKLNVLE

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF**
KRKKPSPSSSFRTEQTATRSWVWTRSQRRAKAKQ
ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR*
QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHW
SPAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR
KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS
SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSE
LFMHLTRMEMVSSSSTFW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSE
KEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQN
KP**AKAVPTATAWQL*SGK*GTATIPEHFQADS
RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG
VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLA
ENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA
L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS
FSCI*QGWRWYHQAQRSG

Sequence CAPN3.lAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGGT
AGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTC
AC
AAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAA
AACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAG
ATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACAC
TGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTG
GCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAAC
CAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCA
GAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTC
ATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCC
AGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAG
AACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGC
TGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGC
ACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTG
ATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Alternative donor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVV
GVWGSVLVIWMLVIGVQY*SGPANPKAYGSWHV
T
KRKKPSPSSSFRTEQTATRSWVWTRSQRRAKA
KQALISKSSPHSHSLAALIRKVRNSNNSGTFSSR
*QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSH
WSPAVA*LRSWIQMALESSTCRSSTTSGTRLRP
GRKFSNTMTQTSPAPSTATRCEMQSTTQDSTSTT
SSMTSLPCGTQTNT*TSTLTVSSAASLGWRACS
ELFMHLTRMEMVSSSSTFWSGCSSPCMPEPGWP
HPKPCRITQDFSFTLYFQSHLPQRTQQLHPYRLP
GTSSVMLLLHFTPYPSLIGHA*PDPLVKQ*GRK
NKPLSLCHVEESACLWSEPPRF*SECSCLPCSR
LSAEAPAGGTQHLLVLEPSITFTLSHHGPGTKPA
LGSTAVG*TNSVE*GWLLWAVQLISLAAF*KKL
I*IKACVWLV

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF*W
*VCGDLFWSSGCWSSVCSIDQDLQTQKLMGAGTS
QKEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQ
NKP**AKAVPTATAWQL*SGK*GTATIPEHFQA
DSRR*HGDLCR*AQEGP*HSREQTQGPEDTRVHT
GVLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GL
AENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQ
PAL*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQ
SFSCI*QGWRWYHQAQRSGVAAAHHVCLNQAGL
IQSHAGSLRISVSPSISKAIYLKGPSSYTPTGF
QAPHQSCSSSILPPTHP*SVMPSLTL**SNEVGR
TNPCPFAMWRKVPASGPSRLGSEASAPAYLALG
CLQKHLPVALSTSLC*SPPSPSRCPTMGQEPNQ
HWVLLLWGKLTQWNRAGYFGLSNS*VWLHFEKS*
SK*RHVYGW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSG
RCVGICSGHLDAGHRCAVLIRTCKPKSLWELAR
H
KKKKTKPIIFVSDRANSNKELGVDQESEEGKGK
TSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQ
IAGDDMEICADELKKVLNTVVNKHKDLKTHGFT
LESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAW
QKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNN
QLYDIITMRYADKHMNIDFDSFICCFVRLEGMF
RAFHAFDKDGDGIIKLNVLEWLQLTMYA*TRLAS
SKAMQDHSGFQFHPLFPKPFTSKDPAATPLQAS
RHLISHAPPPFYPLPILDRSCLA*PFSKAMR*E
EQTLVPLPCGGKCLPLVRAASVLKRVLLLTLL*A
VCRSTCRWHSAPPCARALHHLHAVPPWARNQTS
TGFYCCGVN*LSGIGLVTLGCPTHKFGCILKKA
DLNKGMCMAG

Sequence CAPN3.qAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGGT
AGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTC
AC
AAAAAGAAAAAAACCAAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGA
CATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGT
AGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCA
AACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACAT
CATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCA
TTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGC
AGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAG
TCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTC
CCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCA
CCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTG
CTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGC
ATGTGTATGGCTGGTC
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Alternative donor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVV
GVWGSVLVIWMLVIGVQY*SGPANPKAYGSWHV
T
KRKKPSHSLAALIRKVRNSNNSGTFSSR*QEM
TWRSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAV
A*LRSWIQMALESSTCRSSTTSGTRLRPGRKFS
NTMTQTSPAPSTATRCEMQSTTQDSTSTTSSMT
SLPCGTQTNT*TSTLTVSSAASLGWRACSELFMH
LTRMEMVSSSSTFWSGCSSPCMPEPGWPHPKPC
RITQDFSFTLYFQSHLPQRTQQLHPYRLPGTSS
VMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNKPLS
LCHVEESACLWSEPPRF*SECSCLPCSRLSAEA
PAGGTQHLLVLEPSITFTLSHHGPGTKPALGST
AVG*TNSVE*GWLLWAVQLISLAAF*KKLI*IKA
CVWLV

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF*W
*VCGDLFWSSGCWSSVCSIDQDLQTQKLMGAGTS
QKEKNQATAWQL*SGK*GTATIPEHFQADSRR*
HGDLCR*AQEGP*HSREQTQGPEDTRVHTGVLP
*HDCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQ
TL*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*H
HYHAVRRQTHEHRL*QFHLLLR*AGGHVQSFSC
I*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQSHA
GSLRISVSPSISKAIYLKGPSSYTPTGFQAPHQ
SCSSSILPPTHP*SVMPSLTL**SNEVGRTNPC
PFAMWRKVPASGPSRLGSEASAPAYLALGCLQKH
LPVALSTSLC*SPPSPSRCPTMGQEPNQHWVLL
LWGKLTQWNRAGYFGLSNS*VWLHFEKS*SK*R
HVYGW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSG
RCVGICSGHLDAGHRCAVLIRTCKPKSLWELAR
H
KKKKTKPQPGSSDQESEEQQQFRNIFKQIAGDD
MEICADELKKVLNTVVNKHKDLKTHGFTLESCR
SMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIF
KHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDI
ITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA
FDKDGDGIIKLNVLEWLQLTMYA*TRLASSKAM
QDHSGFQFHPLFPKPFTSKDPAATPLQASRHLIS
HAPPPFYPLPILDRSCLA*PFSKAMR*EEQTLV
PLPCGGKCLPLVRAASVLKRVLLLTLL*AVCRS
TCRWHSAPPCARALHHLHAVPPWARNQTSTGFYC
CGVN*LSGIGLVTLGCPTHKFGCILKKADLNKG
MCMAG

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