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Sequences

Sequence CAPN3.qAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGGT AGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTC ACAAAAAGAAAAAAACCAAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGA CATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGT AGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCA AACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACAT CATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCA TTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGC AGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAG TCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTC CCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCA CCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTG CTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGC ATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVV GVWGSVLVIWMLVIGVQY*SGPANPKAYGSWHV TKRKKPSHSLAALIRKVRNSNNSGTFSSR*QEM TWRSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAV A*LRSWIQMALESSTCRSSTTSGTRLRPGRKFS NTMTQTSPAPSTATRCEMQSTTQDSTSTTSSMT SLPCGTQTNT*TSTLTVSSAASLGWRACSELFMH LTRMEMVSSSSTFWSGCSSPCMPEPGWPHPKPC RITQDFSFTLYFQSHLPQRTQQLHPYRLPGTSS VMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNKPLS LCHVEESACLWSEPPRF*SECSCLPCSRLSAEA PAGGTQHLLVLEPSITFTLSHHGPGTKPALGST AVG*TNSVE*GWLLWAVQLISLAAF*KKLI*IKA CVWLV

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT* VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF*W *VCGDLFWSSGCWSSVCSIDQDLQTQKLMGAGTS QKEKNQATAWQL*SGK*GTATIPEHFQADSRR* HGDLCR*AQEGP*HSREQTQGPEDTRVHTGVLP *HDCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQ TL*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*H HYHAVRRQTHEHRL*QFHLLLR*AGGHVQSFSC I*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQSHA GSLRISVSPSISKAIYLKGPSSYTPTGFQAPHQ SCSSSILPPTHP*SVMPSLTL**SNEVGRTNPC PFAMWRKVPASGPSRLGSEASAPAYLALGCLQKH LPVALSTSLC*SPPSPSRCPTMGQEPNQHWVLL LWGKLTQWNRAGYFGLSNS*VWLHFEKS*SK*R HVYGW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE EG*EVLGMLEILG*HGQESHILSLATTDELIAP ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG* *CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSG RCVGICSGHLDAGHRCAVLIRTCKPKSLWELAR HKKKKTKPQPGSSDQESEEQQQFRNIFKQIAGDD MEICADELKKVLNTVVNKHKDLKTHGFTLESCR SMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIF KHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDI ITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA FDKDGDGIIKLNVLEWLQLTMYA*TRLASSKAM QDHSGFQFHPLFPKPFTSKDPAATPLQASRHLIS HAPPPFYPLPILDRSCLA*PFSKAMR*EEQTLV PLPCGGKCLPLVRAASVLKRVLLLTLL*AVCRS TCRWHSAPPCARALHHLHAVPPWARNQTSTGFYC CGVN*LSGIGLVTLGCPTHKFGCILKKADLNKG MCMAG

Sequence CAPN3.lAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGGT AGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTC ACAAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAA AACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAG ATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACAC TGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTG GCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAAC CAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCA GAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTC ATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCC AGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAG AACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGC TGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGC ACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTG ATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVV GVWGSVLVIWMLVIGVQY*SGPANPKAYGSWHV TKRKKPSPSSSFRTEQTATRSWVWTRSQRRAKA KQALISKSSPHSHSLAALIRKVRNSNNSGTFSSR *QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSH WSPAVA*LRSWIQMALESSTCRSSTTSGTRLRP GRKFSNTMTQTSPAPSTATRCEMQSTTQDSTSTT SSMTSLPCGTQTNT*TSTLTVSSAASLGWRACS ELFMHLTRMEMVSSSSTFWSGCSSPCMPEPGWP HPKPCRITQDFSFTLYFQSHLPQRTQQLHPYRLP GTSSVMLLLHFTPYPSLIGHA*PDPLVKQ*GRK NKPLSLCHVEESACLWSEPPRF*SECSCLPCSR LSAEAPAGGTQHLLVLEPSITFTLSHHGPGTKPA LGSTAVG*TNSVE*GWLLWAVQLISLAAF*KKL I*IKACVWLV

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT* VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF*W *VCGDLFWSSGCWSSVCSIDQDLQTQKLMGAGTS QKEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQ NKP**AKAVPTATAWQL*SGK*GTATIPEHFQA DSRR*HGDLCR*AQEGP*HSREQTQGPEDTRVHT GVLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GL AENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQ PAL*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQ SFSCI*QGWRWYHQAQRSGVAAAHHVCLNQAGL IQSHAGSLRISVSPSISKAIYLKGPSSYTPTGF QAPHQSCSSSILPPTHP*SVMPSLTL**SNEVGR TNPCPFAMWRKVPASGPSRLGSEASAPAYLALG CLQKHLPVALSTSLC*SPPSPSRCPTMGQEPNQ HWVLLLWGKLTQWNRAGYFGLSNS*VWLHFEKS* SK*RHVYGW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE EG*EVLGMLEILG*HGQESHILSLATTDELIAP ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG* *CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSG RCVGICSGHLDAGHRCAVLIRTCKPKSLWELAR HKKKKTKPIIFVSDRANSNKELGVDQESEEGKGK TSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQ IAGDDMEICADELKKVLNTVVNKHKDLKTHGFT LESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAW QKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNN QLYDIITMRYADKHMNIDFDSFICCFVRLEGMF RAFHAFDKDGDGIIKLNVLEWLQLTMYA*TRLAS SKAMQDHSGFQFHPLFPKPFTSKDPAATPLQAS RHLISHAPPPFYPLPILDRSCLA*PFSKAMR*E EQTLVPLPCGGKCLPLVRAASVLKRVLLLTLL*A VCRSTCRWHSAPPCARALHHLHAVPPWARNQTS TGFYCCGVN*LSGIGLVTLGCPTHKFGCILKKA DLNKGMCMAG

Sequence CAPN3.pAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGAA AAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACA AGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAG CAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGA GTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAG AAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGC TCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC TTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVK KKKTKPIIFVSDRANSNKELGVDQESEEGKGKT SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQI AGDDMEICADELKKVLNTVVNKHKDLKTHGFTLE SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQ LYDIITMRYADKHMNIDFDSFICCFVRLEGMFRA FHAFDKDGDGIIKLNVLE

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT* VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF** KRKKPSPSSSFRTEQTATRSWVWTRSQRRAKAKQ ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR* QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHW SPAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSE LFMHLTRMEMVSSSSTFW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE EG*EVLGMLEILG*HGQESHILSLATTDELIAP ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG* *CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSE KEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQN KP**AKAVPTATAWQL*SGK*GTATIPEHFQADS RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLA ENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS FSCI*QGWRWYHQAQRSG

Sequence CAPN3.jAug05

CCCTCCTCCACGCTTACAGCCACACACACAGTCACACAGACGCGTTCTGAGGGTGGCTGCCCGCTTGGGATGGAGGAATCACTTCCCTCAGAACCCAGCC AAGTCCTCTAGGCCTCCTTGGGGGTCCTTCCAGCCTGAGGGGCTTCGGAGCTGAGGAGCAGCTGTTCTGATGCACGGGAACAAGCAGCACCTGCAGAAGG ACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCAT CGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCC GTGGATCGGCCAGTGAAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGG AGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAA CATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACA CACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACA AGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATT CCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTG GAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAA CCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACC CCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCA ATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTA CCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAG GAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATT TTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PSSTLTATHTVTQTRSEGGCPLGMEESLPSEPS QVL*ASLGVLPA*GASELRSSCSDAREQAAPAEG LLPVQRLQGQEQNLHQHAGGVPALPPASQRVRH RALHLRAPPGGGIHPPGLL*KEEPL*GS*KYHL RGSASEKEKNQAHHLRFGQSKQQQGAGCGPGVRG GQRQNKP**AKAVPTATAWQL*SGK*GTATIPE HFQADSRR*HGDLCR*AQEGP*HSREQTQGPED TRVHTGVLP*HDCAHGYRWLWKAQPAGVPPPLEQ D*GLAENFQTL*HRPVRHHQQLRDAKCSQRRRI PPQQPAL*HHYHAVRRQTHEHRL*QFHLLLR*A GGHVQSFSCI*QGWRWYHQAQRSGVAAAHHVCLN QAGLIQSHAGSLRISVSPSISKAIYLKGPSSYT PTGFQAPHQSCSSSILPPTHP*SVMPSLTL**S NEVGRTNPCPFAMWRKVPASGPSRLGSEASAPAY LALGCLQKHLPVALSTSLC*SPPSPSRCPTMGQ EPNQHWVLLLWGKLTQWNRAGYFGLSNS*VWLH FEKS*SK*RHVYGW

Frame 1

PPPRLQPHTQSHRRVLRVAARLGWRNHFPQNPA KSSRPPWGSFQPEGLRS*GAAVLMHGNKQHLQK DFFLYNASKARSKTYINMREVSQRFRLPPSEYVI VPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS VDRPVKKKKTKPIIFVSDRANSNKELGVDQESE EGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRN IFKQIAGDDMEICADELKKVLNTVVNKHKDLKT HGFTLESCRSMIALMDTDGSGKLNLQEFHHLWN KIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGF HLNNQLYDIITMRYADKHMNIDFDSFICCFVRL EGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA* TRLASSKAMQDHSGFQFHPLFPKPFTSKDPAATP LQASRHLISHAPPPFYPLPILDRSCLA*PFSKA MR*EEQTLVPLPCGGKCLPLVRAASVLKRVLLL TLL*AVCRSTCRWHSAPPCARALHHLHAVPPWAR NQTSTGFYCCGVN*LSGIGLVTLGCPTHKFGCI LKKADLNKGMCMAG

Frame 2

LLHAYSHTHSHTDAF*GWLPAWDGGITSLRTQP SPLGLLGGPSSLRGFGAEEQLF*CTGTSSTCRR TSSCTTPPRPGAKPTSTCGRCPSASACLPASTS SCPPPTSPTRRGNSSSGSSLKRGTSLRKLKIPSP WIGQ*KRKKPSPSSSFRTEQTATRSWVWTRSQR RAKAKQALISKSSPHSHSLAALIRKVRNSNNSG TFSSR*QEMTWRSVQMSSRRSLTQS*TNTRT*RH TGSHWSPAVA*LRSWIQMALESSTCRSSTTSGT RLRPGRKFSNTMTQTSPAPSTATRCEMQSTTQD STSTTSSMTSLPCGTQTNT*TSTLTVSSAASLGW RACSELFMHLTRMEMVSSSSTFWSGCSSPCMPE PGWPHPKPCRITQDFSFTLYFQSHLPQRTQQLH PYRLPGTSSVMLLLHFTPYPSLIGHA*PDPLVKQ *GRKNKPLSLCHVEESACLWSEPPRF*SECSCL PCSRLSAEAPAGGTQHLLVLEPSITFTLSHHGP GTKPALGSTAVG*TNSVE*GWLLWAVQLISLAAF *KKLI*IKACVWLV

Sequence CAPN3.iAug05

CCCCATCTCTCAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGG GAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCT CTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGAAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAG AGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCT GGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGA AGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGG CTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACC ATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACA TGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAA GCTCAACGTTCTGGAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGG GCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCT GCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PHLSDAREQAAPAEGLLPVQRLQGQEQNLHQHA GGVPALPPASQRVRHRALHLRAPPGGGIHPPGLL *KEEPL*GS*KYHLRGSASEKEKNQAHHLRFGQ SKQQQGAGCGPGVRGGQRQNKP**AKAVPTATA WQL*SGK*GTATIPEHFQADSRR*HGDLCR*AQE GP*HSREQTQGPEDTRVHTGVLP*HDCAHGYRW LWKAQPAGVPPPLEQD*GLAENFQTL*HRPVRH HQQLRDAKCSQRRRIPPQQPAL*HHYHAVRRQTH EHRL*QFHLLLR*AGGHVQSFSCI*QGWRWYHQ AQRSGGCLQKHLPVALSTSLC*SPPSPSRCPTM GQEPNQHWVLLLWGKLTQWNRAGYFGLSNS*VWL HFEKS*SK*RHVYGW

Frame 1

PISQMHGNKQHLQKDFFLYNASKARSKTYINMR EVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVF SEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDR ANSNKELGVDQESEEGKGKTSPDKQKQSPQPQP GSSDQESEEQQQFRNIFKQIAGDDMEICADELK KVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDG SGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGT INSYEMRNAVNDAGFHLNNQLYDIITMRYADKH MNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIK LNVLEAVCRSTCRWHSAPPCARALHHLHAVPPW ARNQTSTGFYCCGVN*LSGIGLVTLGCPTHKFG CILKKADLNKGMCMAG

Frame 2

PSLRCTGTSSTCRRTSSCTTPPRPGAKPTSTCG RCPSASACLPASTSSCPPPTSPTRRGNSSSGSS LKRGTSLRKLKIPSPWIGQ*KRKKPSPSSSFRT EQTATRSWVWTRSQRRAKAKQALISKSSPHSHSL AALIRKVRNSNNSGTFSSR*QEMTWRSVQMSSR RSLTQS*TNTRT*RHTGSHWSPAVA*LRSWIQM ALESSTCRSSTTSGTRLRPGRKFSNTMTQTSPAP STATRCEMQSTTQDSTSTTSSMTSLPCGTQTNT *TSTLTVSSAASLGWRACSELFMHLTRMEMVSS SSTFWRLSAEAPAGGTQHLLVLEPSITFTLSHHG PGTKPALGSTAVG*TNSVE*GWLLWAVQLISLA AF*KKLI*IKACVWLV

Sequence CAPN3.bAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC CATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACTCACTG CTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCA GAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTG GAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGG ATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGT CTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCT GGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGT CTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGG CCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCA GGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGAAAAAGAAAAAA ACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATA AGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGA CATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGT AGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCA AACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACAT CATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCA TTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGC AGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAG TCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTC CCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCA CCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTG CTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGC ATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV WKRPPEICENPRFIIDGANRTDICQGELGDCWF LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS IDDGTNMTYGTSPSGLNMGELIARMVRNMDNSL LQDSDLDPRGSDERPTRTIIPVQYETRMACGLV RGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEW NGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFW MSYEDFIYHFTKLEICNLTADALQSDKLQTWTV SVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLL EEDDDPDDSEVICSFLVALMQKNRRKDRKLGAS LFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASK ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQ EGEFILRVFSEKRNLSEEVENTISVDRPVKKKK TKPIIFVSDRANSNKELGVDQESEEGKGKTSPD KQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDD MEICADELKKVLNTVVNKHKDLKTHGFTLESCR SMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIF KHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDI ITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA FDKDGDGIIKLNVLEWLQLTMYA*TRLASSKAM QDHSGFQFHPLFPKPFTSKDPAATPLQASRHLIS HAPPPFYPLPILDRSCLA*PFSKAMR*EEQTLV PLPCGGKCLPLVRAASVLKRVLLLTLL*AVCRS TCRWHSAPPCARALHHLHAVPPWARNQTSTGFYC CGVN*LSGIGLVTLGCPTHKFGCILKKADLNKG MCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA PLMMART*PMEPLLLV*TWGS*LHGW*GIWITHC SRTQTSTPEAQMKDRPGQSFRFSMRQEWPAGWS EVTPTLSRGWMRSRSKVRK*SWCGCGIRGARWS GTVLGVIDGRTGALWTKMRRPVCSTRSLRMESSG CPMRISSTISQSWRSATSRPMLCSLTSFRPGQC L*TRAAGYGVALPEAAATSQILSGPTLSTV*SS WRRTMTLMTRR*FAASWWP*CRRTGGRTGS*GPV SSPLASPSTRFPKRCTGTSSTCRRTSSCTTPPR PGAKPTSTCGRCPSASACLPASTSSCPPPTSPT RRGNSSSGSSLKRGTSLRKLKIPSPWIGQ*KRKK PSPSSSFRTEQTATRSWVWTRSQRRAKAKQALI SKSSPHSHSLAALIRKVRNSNNSGTFSSR*QEM TWRSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAV A*LRSWIQMALESSTCRSSTTSGTRLRPGRKFS NTMTQTSPAPSTATRCEMQSTTQDSTSTTSSMT SLPCGTQTNT*TSTLTVSSAASLGWRACSELFMH LTRMEMVSSSSTFWSGCSSPCMPEPGWPHPKPC RITQDFSFTLYFQSHLPQRTQQLHPYRLPGTSS VMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNKPLS LCHVEESACLWSEPPRF*SECSCLPCSRLSAEA PAGGTQHLLVLEPSITFTLSHHGPGTKPALGST AVG*TNSVE*GWLLWAVQLISLAAF*KKLI*IKA CVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH *GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ *VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL H**WHEHDLWNLSFWSEHGGVDCTDGKEYG*LT APGLRPRPQRLR*KTDPDNHSGSV*DKNGLRAGQ RSRLLCHGAG*GPVQR*ESEAGAAAESVGPGGV ERFLE**MEGLELCGQR*EGPSAAPGH*GWRVL DVL*GFHLPFHKVGDLQPHGRCSAV*QASDLDSV CERGPLGTGLLCRRLPQLPRYFLDQPSVPSEAP GGGR*P**LGGDLQLPGGPDAEEPAEGPEARGQ SLHHWLRHLRGSQRDAREQAAPAEGLLPVQRLQG QEQNLHQHAGGVPALPPASQRVRHRALHLRAPP GGGIHPPGLL*KEEPL*GS*KYHLRGSASEKEK NQAHHLRFGQSKQQQGAGCGPGVRGGQRQNKP** AKAVPTATAWQL*SGK*GTATIPEHFQADSRR* HGDLCR*AQEGP*HSREQTQGPEDTRVHTGVLP *HDCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQ TL*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*H HYHAVRRQTHEHRL*QFHLLLR*AGGHVQSFSC I*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQSHA GSLRISVSPSISKAIYLKGPSSYTPTGFQAPHQ SCSSSILPPTHP*SVMPSLTL**SNEVGRTNPC PFAMWRKVPASGPSRLGSEASAPAYLALGCLQKH LPVALSTSLC*SPPSPSRCPTMGQEPNQHWVLL LWGKLTQWNRAGYFGLSNS*VWLHFEKS*SK*R HVYGW