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Sequences

Sequence CAPN3.oAug05

CCCTCCTCCACGCTTACAGCCACACACACAGTCACACAGACGCGTTCTGAGGGTGGCTGCCCGCTTGGGATGGAGGAATCACTTCCCTCAGAACCCAGCC
AAGTCCTCTAGGCCTCCTTGGGGGTCCTTCCAGCCTGAGGGGCTTCGGAGCTGAGGAGCAGCTGTTCTGATGCACGGGAACAAGCAGCACCTGCAGAAGG
ACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCAT
CGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCC
GTGGATCGGCCAGTGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGG
AGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCAT
GATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACAC
TATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTA
CCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGA
CAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

PSSTLTATHTVTQTRSEGGCPLGMEESLPSEPS
QVL*ASLGVLPA*GASELRSSCSDAREQAAPAEG
LLPVQRLQGQEQNLHQHAGGVPALPPASQRVRH
RALHLRAPPGGGIHPPGLL*KEEPL*GS*KYHL
RGSASATAWQL*SGK*GTATIPEHFQADSRR*HG
DLCR*AQEGP*HSREQTQGPEDTRVHTGVLP*H
DCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQT
L*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*HHY
HAVRRQTHEHRL*QFHLLLR*AGGHVQSFSCI*
QGWRWYHQAQRSG

Frame 1

PPPRLQPHTQSHRRVLRVAARLGWRNHFPQNPA
KSSRPPWGSFQPEGLRS*GAAVLMHGNKQHLQK
DFFLYNASKARSKTYINMREVSQRFRLPPSEYVI
VPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS
VDRPVPQPGSSDQESEEQQQFRNIFKQIAGDDM
EICADELKKVLNTVVNKHKDLKTHGFTLESCRSM
IALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKH
YDTDQSGTINSYEMRNAVNDAGFHLNNQLYDII
TMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFD
KDGDGIIKLNVLE

Frame 2

LLHAYSHTHSHTDAF*GWLPAWDGGITSLRTQP
SPLGLLGGPSSLRGFGAEEQLF*CTGTSSTCRR
TSSCTTPPRPGAKPTSTCGRCPSASACLPASTS
SCPPPTSPTRRGNSSSGSSLKRGTSLRKLKIPSP
WIGQCHSLAALIRKVRNSNNSGTFSSR*QEMTW
RSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAVA
*LRSWIQMALESSTCRSSTTSGTRLRPGRKFSNT
MTQTSPAPSTATRCEMQSTTQDSTSTTSSMTSL
PCGTQTNT*TSTLTVSSAASLGWRACSELFMHL
TRMEMVSSSSTFW

Sequence CAPN3.nAug05

CCCCATCTCTCAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGG
GAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCT
CTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCA
ACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAG
GACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACC
ACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAA
CGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGC
TTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

PHLSDAREQAAPAEGLLPVQRLQGQEQNLHQHA
GGVPALPPASQRVRHRALHLRAPPGGGIHPPGLL
*KEEPL*GS*KYHLRGSASATAWQL*SGK*GTA
TIPEHFQADSRR*HGDLCR*AQEGP*HSREQTQ
GPEDTRVHTGVLP*HDCAHGYRWLWKAQPAGVPP
PLEQD*GLAENFQTL*HRPVRHHQQLRDAKCSQ
RRRIPPQQPAL*HHYHAVRRQTHEHRL*QFHLL
LR*AGGHVQSFSCI*QGWRWYHQAQRSG

Frame 1

PISQMHGNKQHLQKDFFLYNASKARSKTYINMR
EVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVF
SEKRNLSEEVENTISVDRPVPQPGSSDQESEEQQ
QFRNIFKQIAGDDMEICADELKKVLNTVVNKHK
DLKTHGFTLESCRSMIALMDTDGSGKLNLQEFH
HLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVN
DAGFHLNNQLYDIITMRYADKHMNIDFDSFICC
FVRLEGMFRAFHAFDKDGDGIIKLNVLE

Frame 2

PSLRCTGTSSTCRRTSSCTTPPRPGAKPTSTCG
RCPSASACLPASTSSCPPPTSPTRRGNSSSGSS
LKRGTSLRKLKIPSPWIGQCHSLAALIRKVRNS
NNSGTFSSR*QEMTWRSVQMSSRRSLTQS*TNTR
T*RHTGSHWSPAVA*LRSWIQMALESSTCRSST
TSGTRLRPGRKFSNTMTQTSPAPSTATRCEMQS
TTQDSTSTTSSMTSLPCGTQTNT*TSTLTVSSAA
SLGWRACSELFMHLTRMEMVSSSSTFW

Sequence CAPN3.dAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC
TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT
CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA
CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA
CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA
CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC
TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC
TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT
CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT
GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA
CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC
CATTGATACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTC
AAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGG
ACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGAT
CTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGC
TGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCT
TCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGAT
GCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGC
TTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACC
TCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACAT
TTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACAC
GGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGA
TTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCA
CCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAG
GGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCA
GGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCT
ACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATG
AGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCT
TGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAA
CCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTG
AAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE
LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL
R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP
LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA
TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH
KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV
WKRPPEICENPRFIIDGANRTDICQGELGDCWF
LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF
QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN
EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF
TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS
IDTIIPVQYETRMACGLVRGHAYSVTGLDEVPF
KGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFV
DKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEI
CNLTADALQSDKLQTWTVSVNEGRWVRGCSAGG
CRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICS
FLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEM
HGNKQHLQKDFFLYNASKARSKTYINMREVSQR
FRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRN
LSEEVENTISVDRPVPQPGSSDQESEEQQQFRNI
FKQIAGDDMEICADELKKVLNTVVNKHKDLKTH
GFTLESCRSMIALMDTDGSGKLNLQEFHHLWNK
IKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFH
LNNQLYDIITMRYADKHMNIDFDSFICCFVRLE
GMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA*T
RLASSKAMQDHSGFQFHPLFPKPFTSKDPAATPL
QASRHLISHAPPPFYPLPILDRSCLA*PFSKAM
R*EEQTLVPLPCGGKCLPLVRAASVLKRVLLLT
LL*AVCRSTCRWHSAPPCARALHHLHAVPPWARN
QTSTGFYCCGVN*LSGIGLVTLGCPTHKFGCIL
KKADLNKGMCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN
LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL
SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH
LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP
LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF
TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS
GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF
SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST
SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM
SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS
QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA
PLIQSFRFSMRQEWPAGWSEVTPTLSRGWMRSRS
KVRK*SWCGCGIRGARWSGTVLGVIDGRTGALW
TKMRRPVCSTRSLRMESSGCPMRISSTISQSWR
SATSRPMLCSLTSFRPGQCL*TRAAGYGVALPEA
AATSQILSGPTLSTV*SSWRRTMTLMTRR*FAA
SWWP*CRRTGGRTGS*GPVSSPLASPSTRFPKR
CTGTSSTCRRTSSCTTPPRPGAKPTSTCGRCPSA
SACLPASTSSCPPPTSPTRRGNSSSGSSLKRGT
SLRKLKIPSPWIGQCHSLAALIRKVRNSNNSGT
FSSR*QEMTWRSVQMSSRRSLTQS*TNTRT*RHT
GSHWSPAVA*LRSWIQMALESSTCRSSTTSGTR
LRPGRKFSNTMTQTSPAPSTATRCEMQSTTQDS
TSTTSSMTSLPCGTQTNT*TSTLTVSSAASLGWR
ACSELFMHLTRMEMVSSSSTFWSGCSSPCMPEP
GWPHPKPCRITQDFSFTLYFQSHLPQRTQQLHP
YRLPGTSSVMLLLHFTPYPSLIGHA*PDPLVKQ*
GRKNKPLSLCHVEESACLWSEPPRF*SECSCLP
CSRLSAEAPAGGTQHLLVLEPSITFTLSHHGPG
TKPALGSTAVG*TNSVE*GWLLWAVQLISLAAF*
KKLI*IKACVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT
YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS
QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA
TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH
*GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS
QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR
LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS
RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL
PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ
*VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH
RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL
H*YNHSGSV*DKNGLRAGQRSRLLCHGAG*GPV
QR*ESEAGAAAESVGPGGVERFLE**MEGLELCG
QR*EGPSAAPGH*GWRVLDVL*GFHLPFHKVGD
LQPHGRCSAV*QASDLDSVCERGPLGTGLLCRR
LPQLPRYFLDQPSVPSEAPGGGR*P**LGGDLQL
PGGPDAEEPAEGPEARGQSLHHWLRHLRGSQRD
AREQAAPAEGLLPVQRLQGQEQNLHQHAGGVPA
LPPASQRVRHRALHLRAPPGGGIHPPGLL*KEEP
L*GS*KYHLRGSASATAWQL*SGK*GTATIPEH
FQADSRR*HGDLCR*AQEGP*HSREQTQGPEDT
RVHTGVLP*HDCAHGYRWLWKAQPAGVPPPLEQD
*GLAENFQTL*HRPVRHHQQLRDAKCSQRRRIP
PQQPAL*HHYHAVRRQTHEHRL*QFHLLLR*AG
GHVQSFSCI*QGWRWYHQAQRSGVAAAHHVCLNQ
AGLIQSHAGSLRISVSPSISKAIYLKGPSSYTP
TGFQAPHQSCSSSILPPTHP*SVMPSLTL**SN
EVGRTNPCPFAMWRKVPASGPSRLGSEASAPAYL
ALGCLQKHLPVALSTSLC*SPPSPSRCPTMGQE
PNQHWVLLLWGKLTQWNRAGYFGLSNS*VWLHF
EKS*SK*RHVYGW

Sequence CAPN3.pAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGAA
AAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACA
AGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAG
CAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGA
GTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAG
AAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGC
TCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC
TTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVK
KKKTKPIIFVSDRANSNKELGVDQESEEGKGKT
SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQI
AGDDMEICADELKKVLNTVVNKHKDLKTHGFTLE
SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ
KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQ
LYDIITMRYADKHMNIDFDSFICCFVRLEGMFRA
FHAFDKDGDGIIKLNVLE

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF**
KRKKPSPSSSFRTEQTATRSWVWTRSQRRAKAKQ
ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR*
QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHW
SPAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR
KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS
SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSE
LFMHLTRMEMVSSSSTFW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSE
KEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQN
KP**AKAVPTATAWQL*SGK*GTATIPEHFQADS
RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG
VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLA
ENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA
L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS
FSCI*QGWRWYHQAQRSG

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