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Sequences

Sequence CAPN3.rAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGGT
AGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTC
ACCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGA
TGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATG
GATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACC
AGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGC
AGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGAT
GGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTC
AGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATT
TTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGA
AAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAG
CACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACT
CAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVV
GVWGSVLVIWMLVIGVQY*SGPANPKAYGSWHV
THSLAALIRKVRNSNNSGTFSSR*QEMTWRSVQ
MSSRRSLTQS*TNTRT*RHTGSHWSPAVA*LRSW
IQMALESSTCRSSTTSGTRLRPGRKFSNTMTQT
SPAPSTATRCEMQSTTQDSTSTTSSMTSLPCGT
QTNT*TSTLTVSSAASLGWRACSELFMHLTRMEM
VSSSSTFWSGCSSPCMPEPGWPHPKPCRITQDF
SFTLYFQSHLPQRTQQLHPYRLPGTSSVMLLLH
FTPYPSLIGHA*PDPLVKQ*GRKNKPLSLCHVEE
SACLWSEPPRF*SECSCLPCSRLSAEAPAGGTQ
HLLVLEPSITFTLSHHGPGTKPALGSTAVG*TN
SVE*GWLLWAVQLISLAAF*KKLI*IKACVWLV

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF*W
*VCGDLFWSSGCWSSVCSIDQDLQTQKLMGAGTS
PTAWQL*SGK*GTATIPEHFQADSRR*HGDLCR
*AQEGP*HSREQTQGPEDTRVHTGVLP*HDCAH
GYRWLWKAQPAGVPPPLEQD*GLAENFQTL*HRP
VRHHQQLRDAKCSQRRRIPPQQPAL*HHYHAVR
RQTHEHRL*QFHLLLR*AGGHVQSFSCI*QGWR
WYHQAQRSGVAAAHHVCLNQAGLIQSHAGSLRIS
VSPSISKAIYLKGPSSYTPTGFQAPHQSCSSSI
LPPTHP*SVMPSLTL**SNEVGRTNPCPFAMWR
KVPASGPSRLGSEASAPAYLALGCLQKHLPVALS
TSLC*SPPSPSRCPTMGQEPNQHWVLLLWGKLT
QWNRAGYFGLSNS*VWLHFEKS*SK*RHVYGW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSG
RCVGICSGHLDAGHRCAVLIRTCKPKSLWELAR
HPQPGSSDQESEEQQQFRNIFKQIAGDDMEICAD
ELKKVLNTVVNKHKDLKTHGFTLESCRSMIALM
DTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTD
QSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYA
DKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGD
GIIKLNVLEWLQLTMYA*TRLASSKAMQDHSGF
QFHPLFPKPFTSKDPAATPLQASRHLISHAPPPF
YPLPILDRSCLA*PFSKAMR*EEQTLVPLPCGG
KCLPLVRAASVLKRVLLLTLL*AVCRSTCRWHS
APPCARALHHLHAVPPWARNQTSTGFYCCGVN*L
SGIGLVTLGCPTHKFGCILKKADLNKGMCMAG

Sequence CAPN3.pAug05

CCATTCTGTGATATTTATTGAGCACCTACTATGTTCCAGGCACTGTCCTAGGCACTAAGGATAGAGTAGTGAAGTAAACAGAAAGAAATCCCTGCCTTCA
TGGAGCTTAATATTCTAACATGAGACAATAATGGATAGGAAAAACATATGTAGCATGTTAGATTTGGAGAGGTGATATGGAGCAAAAATAAAGTAGGGAA
GAGGGATAGGAGGTGTTGGGGATGCTTGAAATTTTAGGTTAGCATGGCCAGGAAAGCCACATCCTGTCCCTGGCCACCACAGATGAGCTCATAGCCCCTG
CCACTCTGATCTCTGTCCTTGGAAGATGCACCAGGTCCATGGGTAGGTGGCTGGGTCATGCCTTTGGGGGGCTCTGAGCAATACTAACAAGAACCTGCGT
GCCTGGGCTTGGCTGTCGGGGATGGTGCTGACATGGGGCTGGTTCCTGGGGTTGGGGTGTTCCAGGGGTTCTCTAGAGGCTGGTTCTGGCTTGGCTGCCA
GGAAGCCGTGCACCAGAGCAAACCGTCCACGGGCCTCCTGCTTGCTTCTGGTGACACTGAGACCCCACATGTCTGTATTCCTCACAGGGAAGTTGAAAAT
ACCATCTCCGTGGATCGGCCAGTGGTGAGTGGTTTAGATCTTCTGTGCGAAAAGTCCAGAGGGTCCCCTTCCCTGACCATGCAGGGGACAGATGGTGCAG
GGGAGAATGGGCACTGGCAGAGGGAATGGGAGTCTGGGCTGTGCTGAGCAGTCCCTCCTTGGCACTGCAAATCCTACTTTGGCATGGCCAGAAGTAATCG
GCCTTAAGCACCGGGGGCCATTGAGGCAGTTCAGGGGCTGGGAAATATGGAAGAGGGTCCTGGAAAGGAGAAGCAATTTGAACAATCGGAGGGAACAAGG
CCACAGGAAGGGATGACAAGAGCCGCAGCGAACACTGGATTCTGAGACTGGATAACATTGGATTTCACACATAGAGAAAAGAAAGTAAGCTGGTGCCGGA
CCTGGTGTTGACACTTGGATCCTCCACTTACCAGCGGGGTGACCTGGACAATTTCTGTAATCCCTCTCACTCAGTTTCCTACTCAGTAAAACGGGGATGA
TAATGTGCCTTGCAAGGCTTTTGTGAGGCTTCATCAATGAGGTGATGTATGTGAAGTGTCTGGCACAGCATGGGCACTCAAACAGAGGTGCTTTTTCACA
CTTTACACCTTACAAGGTACTTTTCACATGTGTCATCGCGATACTTGCAAGGTTGCTGAGAGGTAGATGGGGTTATAATCCCTGGTGTTCAAGAAAGGAA
GCAGAGGCTCAATGGGGTTGAATGACTTCTCTGAGTTCACAGAGCTCAGTAAGTGGCAGGGTTGGAACTCACATTCAGACTCTCTGACTCCAGACTTAGG
TTTTTCCGCACCTCCACGCTGAGGCCAGCCCCAGGCAGTGAGAAGCCCAAAGTCCGAAGCACAGAGTGCTGTGTGTTGGGCTCTGTGTGTTGAGGAGTCT
TGTGACTGCCTTGGGGCTTTGGGCTGTAGTCAGCTGACAGTCCTTTGTGCTCTGTGGGGATGACGTAGGCCAATGGGAGGACAAATGCCCCTCTGAACTG
TCTTCTGGGCAGTGACAGTCATGGTCATAATCCTGACCCTGAGCCAGTGCCAGGTCTCCAAGTGCCTTCTGAATGACCACAGGCGATTGGTTTTAGTGAA
AAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACA
AGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAG
CAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGA
GTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAG
AAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGC
TCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC
TTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAG
  • CAPN3.u.f.52 (forward primer)
  • CAPN3.u.r.55 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

PFCDIY*APTMFQALS*ALRIE**SKQKEIPAF
MELNILT*DNNG*EKHM*HVRFGEVIWSKNKVGK
RDRRCWGCLKF*VSMARKATSCPWPPQMSS*PL
PL*SLSLEDAPGPWVGGWVMPLGGSEQY*QEPA
CLGLAVGDGADMGLVPGVGVFQGFSRGWFWLGCQ
EAVHQSKPSTGLLLASGDTETPHVCIPHREVEN
TISVDRPVVSGLDLLCEKSRGSPSLTMQGTDGA
GENGHWQREWESGLC*AVPPWHCKSYFGMARSNR
P*APGAIEAVQGLGNMEEGPGKEKQFEQSEGTR
PQEGMTRAAANTGF*DWITLDFTHREKKVSWCR
TWC*HLDPPLTSGVTWTISVIPLTQFPTQ*NGDD
NVPCKAFVRLHQ*GDVCEVSGTAWALKQRCFFT
LYTLQGTFHMCHRDTCKVAER*MGL*SLVFKKG
SRGSMGLNDFSEFTELSKWQGWNSHSDSLTPDLG
FSAPPR*GQPQAVRSPKSEAQSAVCWALCVEES
CDCLGALGCSQLTVLCALWG*RRPMGGQMPL*T
VFWAVTVMVIILTLSQCQVSKCLLNDHRRLVLVK
KKKTKPIIFVSDRANSNKELGVDQESEEGKGKT
SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQI
AGDDMEICADELKKVLNTVVNKHKDLKTHGFTLE
SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ
KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQ
LYDIITMRYADKHMNIDFDSFICCFVRLEGMFRA
FHAFDKDGDGIIKLNVLE

Frame 1

HSVIFIEHLLCSRHCPRH*G*SSEVNRKKSLPS
WSLIF*HETIMDRKNICSMLDLER*YGAKIK*G
RGIGGVGDA*NFRLAWPGKPHPVPGHHR*AHSPC
HSDLCPWKMHQVHG*VAGSCLWGALSNTNKNLR
AWAWLSGMVLTWGWFLGLGCSRGSLEAGSGLAA
RKPCTRANRPRASCLLLVTLRPHMSVFLTGKLKI
PSPWIGQW*VV*IFCAKSPEGPLP*PCRGQMVQ
GRMGTGRGNGSLGCAEQSLLGTANPTLAWPEVI
GLKHRGPLRQFRGWEIWKRVLERRSNLNNRREQG
HRKG*QEPQRTLDSETG*HWISHIEKRK*AGAG
PGVDTWILHLPAG*PGQFL*SLSLSFLLSKTGM
IMCLARLL*GFINEVMYVKCLAQHGHSNRGAFSH
FTPYKVLFTCVIAILARLLRGRWGYNPWCSRKE
AEAQWG*MTSLSSQSSVSGRVGTHIQTL*LQT*
VFPHLHAEASPRQ*EAQSPKHRVLCVGLCVLRSL
VTALGLWAVVS*QSFVLCGDDVGQWEDKCPSEL
SSGQ*QSWS*S*P*ASARSPSAF*MTTGDWF**
KRKKPSPSSSFRTEQTATRSWVWTRSQRRAKAKQ
ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR*
QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHW
SPAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR
KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS
SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSE
LFMHLTRMEMVSSSSTFW

Frame 2

IL*YLLSTYYVPGTVLGTKDRVVK*TERNPCLH
GA*YSNMRQ*WIGKTYVAC*IWRGDMEQK*SRE
EG*EVLGMLEILG*HGQESHILSLATTDELIAP
ATLISVLGRCTRSMGRWLGHAFGGL*AILTRTCV
PGLGCRGWC*HGAGSWGWGVPGVL*RLVLAWLP
GSRAPEQTVHGPPACFW*H*DPTCLYSSQGS*K
YHLRGSASGEWFRSSVRKVQRVPFPDHAGDRWCR
GEWALAEGMGVWAVLSSPSLALQILLWHGQK*S
ALSTGGH*GSSGAGKYGRGSWKGEAI*TIGGNK
ATGRDDKSRSEHWILRLDNIGFHT*RKESKLVPD
LVLTLGSSTYQRGDLDNFCNPSHSVSYSVKRG*
*CALQGFCEASSMR*CM*SVWHSMGTQTEVLFH
TLHLTRYFSHVSSRYLQGC*EVDGVIIPGVQERK
QRLNGVE*LL*VHRAQ*VAGLELTFRLSDSRLR
FFRTSTLRPAPGSEKPKVRSTECCVLGSVC*GV
L*LPWGFGL*SADSPLCSVGMT*ANGRTNAPLNC
LLGSDSHGHNPDPEPVPGLQVPSE*PQAIGFSE
KEKNQAHHLRFGQSKQQQGAGCGPGVRGGQRQN
KP**AKAVPTATAWQL*SGK*GTATIPEHFQADS
RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG
VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLA
ENFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA
L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS
FSCI*QGWRWYHQAQRSG

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