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Sequences

Sequence CAPN3.hAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC AAGGAGAGCTAGGGGACTGCTGGTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCAT CGAAAACTACGCAGGGATCTTCCACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTG GTTTTCACCAAGTCCAACCACCGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGA ACACCACAGAGGCCATGGAGGACTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCAT CGAGAGAGGCTCCCTCATGGGCTGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGG ATGGTAAGGAATATGGATAACTCACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATG AGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCT GCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCAC CAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGT CTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGAC CAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGG CGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGGTATAGCAGCAGCAGCGGCCAGCAGTTGTGT GCAGCACTACCCAGGGGGCCCCGAGTCTGTCTGTGGCTCGTCGAGAAGCTTCCTGGTGGGGTTTGTGGGCAGGACTGTGATAGGAGAGGGCCTTGCCTGT GTTATTTCCACTGCAGAGCAGGTGCCTCAGGGCATTGCATGACCCATGACTACCACCCCCAGGATGTGCACTTTCTCCCTCGCACCAGACACTGCACGTC ACACACATGCCTTTGCACACTCACCCTCCTCCACGCTTACAGCCACACACACAGTCACACAGACGCGTTCTGAGGGTGGCTGCCCGCTTGGGATGGAGGA ATCACTTCCCTCAGAACCCAGCCAAGTCCTCTAGGCCTCCTTGGGGGTCCTTCCAGCCTGAGGGGCTTCGGAGCTGAGGAGCAGCTGTTCTGGTAAGTGT CCCTGAGTGTGGGGATGACACATTTGCCATTCACTCTGAATCACAACAGAAAAGGAAGAGGAAGTGAGGTAGGGAGGCTATTTAAGCCTTGGGAGTCGGA AGTAGGGAGGTTGAAACTGTGACATGGGTGACCAGGGAGTTGGGAAGGGACCCTTGGAGGTGGCTGTGGCAGGACAGGATGTTCCTCCCGAGGGGCTCAT GTGCCCTGGGCTCTCCCCATCTCTCAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCT ACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCAT CCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCATCTTCGTTTCGGACAGAGCA AACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCA GCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGT CCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCT GGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCA ACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAA CATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTC AACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTAT TTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCAT CCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTG GTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTA GAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCT GGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV KES*GTAGFSQPLPA*P*TSTFFSESYPMIKVS SKTTQGSSTSSSGAMESGWTWL*MTACQRTTINW FSPSPTTAMSSGVLCWRRLMLSSMVPTKL*KVG TPQRPWRTSQEGWQSFLRSGMLLVTCTRS*RKP SREAPSWAAPLMMART*PMEPLLLV*TWGS*LHG W*GIWITHCSRTQTSTPEAQMKDRPGQSFRFSM RQEWPAGWSEVTPTLSRGWMRSRSKVRK*SWCG CGIRGARWSGTVLGVIDGRTGALWTKMRRPVCST RSLRMESSGCPMRISSTISQSWRSATSRPMLCS LTSFRPGQCL*TRAAGYGVALPEAAATSQILSG PTLSTV*SSWRRTMTLMTRR*FAASWWP*CRRTG GRTGS*GPVSSPLASPSTRFPKRYSSSSGQQLC AALPRGPRVCLWLVEKLPGGVCGQDCDRRGPCL CYFHCRAGASGHCMTHDYHPQDVHFLPRTRHCTS HTCLCTLTLLHAYSHTHSHTDAF*GWLPAWDGG ITSLRTQPSPLGLLGGPSSLRGFGAEEQLFW*V SLSVGMTHLPFTLNHNRKGRGSEVGRLFKPWESE VGRLKL*HG*PGSWEGTLGGGCGRTGCSSRGAH VPWALPISQMHGNKQHLQKDFFLYNASKARSKT YINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFI LRVFSEKRNLSEEVENTISVDRPVPIIFVSDRA NSNKELGVDQESEEGKGKTSPDKQKQSPQPQPG SSDQESEEQQQFRNIFKQIAGDDMEICADELKKV LNTVVNKHKDLKTHGFTLESCRSMIALMDTDGS GKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTI NSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMN IDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKL NVLEWLQLTMYA*TRLASSKAMQDHSGFQFHPL FPKPFTSKDPAATPLQASRHLISHAPPPFYPLPI LDRSCLA*PFSKAMR*EEQTLVPLPCGGKCLPL VRAASVLKRVLLLTLL*AVCRSTCRWHSAPPCA RALHHLHAVPPWARNQTSTGFYCCGVN*LSGIGL VTLGCPTHKFGCILKKADLNKGMCMAG

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS RRARGLLVSRSHCLPDPEPAPSFPSHTP*SKFH RKLRRDLPLPVLALWRVGGRGYR*LPANVQQST GFHQVQPPQ*VLECSAGEGLC*APWFLRSSERWE HHRGHGGLHRRGGRVF*DQGCS**HVQDHEESH RERLPHGLLH**WHEHDLWNLSFWSEHGGVDCT DGKEYG*LTAPGLRPRPQRLR*KTDPDNHSGSV* DKNGLRAGQRSRLLCHGAG*GPVQR*ESEAGAA AESVGPGGVERFLE**MEGLELCGQR*EGPSAA PGH*GWRVLDVL*GFHLPFHKVGDLQPHGRCSAV *QASDLDSVCERGPLGTGLLCRRLPQLPRYFLD QPSVPSEAPGGGR*P**LGGDLQLPGGPDAEEP AEGPEARGQSLHHWLRHLRGSQRGIAAAAASSCV QHYPGGPESVCGSSRSFLVGFVGRTVIGEGLAC VISTAEQVPQGIA*PMTTTPRMCTFSLAPDTAR HTHAFAHSPSSTLTATHTVTQTRSEGGCPLGMEE SLPSEPSQVL*ASLGVLPA*GASELRSSCSGKC P*VWG*HICHSL*ITTEKEEEVR*GGYLSLGSR K*GG*NCDMGDQGVGKGPLEVAVAGQDVPPEGLM CPGLSPSLRCTGTSSTCRRTSSCTTPPRPGAKP TSTCGRCPSASACLPASTSSCPPPTSPTRRGNS SSGSSLKRGTSLRKLKIPSPWIGQCPSSSFRTEQ TATRSWVWTRSQRRAKAKQALISKSSPHSHSLA ALIRKVRNSNNSGTFSSR*QEMTWRSVQMSSRR SLTQS*TNTRT*RHTGSHWSPAVA*LRSWIQMAL ESSTCRSSTTSGTRLRPGRKFSNTMTQTSPAPS TATRCEMQSTTQDSTSTTSSMTSLPCGTQTNT* TSTLTVSSAASLGWRACSELFMHLTRMEMVSSSS TFWSGCSSPCMPEPGWPHPKPCRITQDFSFTLY FQSHLPQRTQQLHPYRLPGTSSVMLLLHFTPYP SLIGHA*PDPLVKQ*GRKNKPLSLCHVEESACLW SEPPRF*SECSCLPCSRLSAEAPAGGTQHLLVL EPSITFTLSHHGPGTKPALGSTAVG*TNSVE*G WLLWAVQLISLAAF*KKLI*IKACVWLV

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS WQISLKTQTTQQKNEICENPRFIIDGANRTDIC QGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFI ENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQL VFTKSNHRNEFWSALLEKAYAKLHGSYEALKGG NTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAI ERGSLMGCSIDDGTNMTYGTSPSGLNMGELIAR MVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQY ETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRL RNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQH QVTEDGEFWMSYEDFIYHFTKLEICNLTADALQ SDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWT NPQYRLKLLEEDDDPDDSEVICSFLVALMQKNR RKDRKLGASLFTIGFAIYEVPKEV*QQQRPAVV CSTTQGAPSLSVARREASWWGLWAGL**ERALPV LFPLQSRCLRALHDP*LPPPGCALSPSHQTLHV THMPLHTHPPPRLQPHTQSHRRVLRVAARLGWR NHFPQNPAKSSRPPWGSFQPEGLRS*GAAVLVSV PECGDDTFAIHSESQQKRKRK*GREAI*ALGVG SREVETVTWVTRELGRDPWRWLWQDRMFLPRGS CALGSPHLSDAREQAAPAEGLLPVQRLQGQEQNL HQHAGGVPALPPASQRVRHRALHLRAPPGGGIH PPGLL*KEEPL*GS*KYHLRGSASAHHLRFGQS KQQQGAGCGPGVRGGQRQNKP**AKAVPTATAWQ L*SGK*GTATIPEHFQADSRR*HGDLCR*AQEG P*HSREQTQGPEDTRVHTGVLP*HDCAHGYRWL WKAQPAGVPPPLEQD*GLAENFQTL*HRPVRHHQ QLRDAKCSQRRRIPPQQPAL*HHYHAVRRQTHE HRL*QFHLLLR*AGGHVQSFSCI*QGWRWYHQA QRSGVAAAHHVCLNQAGLIQSHAGSLRISVSPSI SKAIYLKGPSSYTPTGFQAPHQSCSSSILPPTH P*SVMPSLTL**SNEVGRTNPCPFAMWRKVPAS GPSRLGSEASAPAYLALGCLQKHLPVALSTSLC* SPPSPSRCPTMGQEPNQHWVLLLWGKLTQWNRA GYFGLSNS*VWLHFEKS*SK*RHVYGW

Sequence CAPN3.fAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC AAGGAGAGCTAGACGGGTTCCAGGGCAGCTGCCTACCTGGCCTTCCTTCCAATACAAATCATCTTGGTGGATGGTTCTCTGAGGCTCAGTCTTCGCTGAA GTCAGAAGAGGAATTGGACTCACATTGCAAAGGCACAGGGCAGGGCAGATTTCCTACAGGTGTTAGGAAGAACAACCCAGTTATGATCACCTACTGCTCT GTCTCCATTGAGGCCTAAAAAGGAAGTGAGTTTATACTGCAGTTGGAGGAACTGCCTGCAGCCTTGAGGAAAATGTCTAGTCACAAGGGAGGGACTGCTG GTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTC CACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACC GCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGA CTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGC TGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACT CACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCT GGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTG GAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGT TCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGAC AGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAG CTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGG CCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTC CAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCC CACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCA TCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTC CCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGT GCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGC TCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACAC AGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGG TACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATG GAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGG ATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCT CCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTG GAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCA CTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAAC TAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTG GTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV KES*TGSRAAAYLAFLPIQIILVDGSLRLSLR* SQKRNWTHIAKAQGRADFLQVLGRTTQL*SPTAL SPLRPKKEVSLYCSWRNCLQP*GKCLVTREGLL VSRSHCLPDPEPAPSFPSHTP*SKFHRKLRRDL PLPVLALWRVGGRGYR*LPANVQQSTGFHQVQPP Q*VLECSAGEGLC*APWFLRSSERWEHHRGHGG LHRRGGRVF*DQGCS**HVQDHEESHRERLPHG LLH**WHEHDLWNLSFWSEHGGVDCTDGKEYG*L TAPGLRPRPQRLR*KTDPDNHSGSV*DKNGLRA GQRSRLLCHGAG*GPVQR*ESEAGAAAESVGPG GVERFLE**MEGLELCGQR*EGPSAAPGH*GWRV LDVL*GFHLPFHKVGDLQPHGRCSAV*QASDLD SVCERGPLGTGLLCRRLPQLPRYFLDQPSVPSE APGGGR*P**LGGDLQLPGGPDAEEPAEGPEARG QSLHHWLRHLRGSQRDAREQAAPAEGLLPVQRL QGQEQNLHQHAGGVPALPPASQRVRHRALHLRA PPGGGIHPPGLL*KEEPL*GS*KYHLRGSASAHH LRFGQSKQQQGAGCGPGVRGGQRQNKP**AKAV PTATAWQL*SGK*GTATIPEHFQADSRR*HGDL CR*AQEGP*HSREQTQGPEDTRVHTGVLP*HDCA HGYRWLWKAQPAGVPPPLEQD*GLAENFQTL*H RPVRHHQQLRDAKCSQRRRIPPQQPAL*HHYHA VRRQTHEHRL*QFHLLLR*AGGHVQSFSCI*QGW RWYHQAQRSGVAAAHHVCLNQAGLIQSHAGSLR ISVSPSISKAIYLKGPSSYTPTGFQAPHQSCSS SILPPTHP*SVMPSLTL**SNEVGRTNPCPFAMW RKVPASGPSRLGSEASAPAYLALGCLQKHLPVA LSTSLC*SPPSPSRCPTMGQEPNQHWVLLLWGK LTQWNRAGYFGLSNS*VWLHFEKS*SK*RHVYGW

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS RRARRVPGQLPTWPSFQYKSSWWMVL*GSVFAE VRRGIGLTLQRHRAGQISYRC*EEQPSYDHLLL CLH*GLKRK*VYTAVGGTACSLEENV*SQGRDCW FLAAIACLTLNQHLLFRVIPHDQSFIENYAGIF HFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNH RNEFWSALLEKAYAKLHGSYEALKGGNTTEAMED FTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMG CSIDDGTNMTYGTSPSGLNMGELIARMVRNMDN SLLQDSDLDPRGSDERPTRTIIPVQYETRMACGL VRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQV EWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGE FWMSYEDFIYHFTKLEICNLTADALQSDKLQTWT VSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLK LLEEDDDPDDSEVICSFLVALMQKNRRKDRKLG ASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNAS KARSKTYINMREVSQRFRLPPSEYVIVPSTYEP HQEGEFILRVFSEKRNLSEEVENTISVDRPVPI IFVSDRANSNKELGVDQESEEGKGKTSPDKQKQS PQPQPGSSDQESEEQQQFRNIFKQIAGDDMEIC ADELKKVLNTVVNKHKDLKTHGFTLESCRSMIA LMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDT DQSGTINSYEMRNAVNDAGFHLNNQLYDIITMR YADKHMNIDFDSFICCFVRLEGMFRAFHAFDKD GDGIIKLNVLEWLQLTMYA*TRLASSKAMQDHSG FQFHPLFPKPFTSKDPAATPLQASRHLISHAPP PFYPLPILDRSCLA*PFSKAMR*EEQTLVPLPC GGKCLPLVRAASVLKRVLLLTLL*AVCRSTCRWH SAPPCARALHHLHAVPPWARNQTSTGFYCCGVN *LSGIGLVTLGCPTHKFGCILKKADLNKGMCMA G

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS WQISLKTQTTQQKNEICENPRFIIDGANRTDIC QGELDGFQGSCLPGLPSNTNHLGGWFSEAQSSLK SEEELDSHCKGTGQGRFPTGVRKNNPVMITYCS VSIEA*KGSEFILQLEELPAALRKMSSHKGGTA GFSQPLPA*P*TSTFFSESYPMIKVSSKTTQGSS TSSSGAMESGWTWL*MTACQRTTINWFSPSPTT AMSSGVLCWRRLMLSSMVPTKL*KVGTPQRPWR TSQEGWQSFLRSGMLLVTCTRS*RKPSREAPSWA APLMMART*PMEPLLLV*TWGS*LHGW*GIWIT HCSRTQTSTPEAQMKDRPGQSFRFSMRQEWPAG WSEVTPTLSRGWMRSRSKVRK*SWCGCGIRGARW SGTVLGVIDGRTGALWTKMRRPVCSTRSLRMES SGCPMRISSTISQSWRSATSRPMLCSLTSFRPG QCL*TRAAGYGVALPEAAATSQILSGPTLSTV*S SWRRTMTLMTRR*FAASWWP*CRRTGGRTGS*G PVSSPLASPSTRFPKRCTGTSSTCRRTSSCTTP PRPGAKPTSTCGRCPSASACLPASTSSCPPPTSP TRRGNSSSGSSLKRGTSLRKLKIPSPWIGQCPS SSFRTEQTATRSWVWTRSQRRAKAKQALISKSS PHSHSLAALIRKVRNSNNSGTFSSR*QEMTWRSV QMSSRRSLTQS*TNTRT*RHTGSHWSPAVA*LR SWIQMALESSTCRSSTTSGTRLRPGRKFSNTMT QTSPAPSTATRCEMQSTTQDSTSTTSSMTSLPCG TQTNT*TSTLTVSSAASLGWRACSELFMHLTRM EMVSSSSTFWSGCSSPCMPEPGWPHPKPCRITQ DFSFTLYFQSHLPQRTQQLHPYRLPGTSSVMLLL HFTPYPSLIGHA*PDPLVKQ*GRKNKPLSLCHV EESACLWSEPPRF*SECSCLPCSRLSAEAPAGG TQHLLVLEPSITFTLSHHGPGTKPALGSTAVG*T NSVE*GWLLWAVQLISLAAF*KKLI*IKACVWL V

Sequence CAPN3.eAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC AAGGAGAGCTAGGGGACTGCTGGTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCAT CGAAAACTACGCAGGGATCTTCCACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTG GTTTTCACCAAGTCCAACCACCGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGA ACACCACAGAGGCCATGGAGGACTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCAT CGAGAGAGGCTCCCTCATGGGCTGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGG ATGGTAAGGAATATGGATAACTCACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATG AGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCT GCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCAC CAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGT CTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGAC CAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGG CGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGG ACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCAT CGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCC GTGGATCGGCCAGTGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAA GCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGC AGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAG TCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGA AAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCT CTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCT TTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCA AAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCA CCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAA ACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCT GCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGG GTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTA AATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV KES*GTAGFSQPLPA*P*TSTFFSESYPMIKVS SKTTQGSSTSSSGAMESGWTWL*MTACQRTTINW FSPSPTTAMSSGVLCWRRLMLSSMVPTKL*KVG TPQRPWRTSQEGWQSFLRSGMLLVTCTRS*RKP SREAPSWAAPLMMART*PMEPLLLV*TWGS*LHG W*GIWITHCSRTQTSTPEAQMKDRPGQSFRFSM RQEWPAGWSEVTPTLSRGWMRSRSKVRK*SWCG CGIRGARWSGTVLGVIDGRTGALWTKMRRPVCST RSLRMESSGCPMRISSTISQSWRSATSRPMLCS LTSFRPGQCL*TRAAGYGVALPEAAATSQILSG PTLSTV*SSWRRTMTLMTRR*FAASWWP*CRRTG GRTGS*GPVSSPLASPSTRFPKRCTGTSSTCRR TSSCTTPPRPGAKPTSTCGRCPSASACLPASTS SCPPPTSPTRRGNSSSGSSLKRGTSLRKLKIPSP WIGQCPSSSFRTEQTATRSWVWTRSQRRAKAKQ ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR* QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHWS PAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSEL FMHLTRMEMVSSSSTFWSGCSSPCMPEPGWPHP KPCRITQDFSFTLYFQSHLPQRTQQLHPYRLPG TSSVMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNK PLSLCHVEESACLWSEPPRF*SECSCLPCSRLS AEAPAGGTQHLLVLEPSITFTLSHHGPGTKPAL GSTAVG*TNSVE*GWLLWAVQLISLAAF*KKLI* IKACVWLV

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS RRARGLLVSRSHCLPDPEPAPSFPSHTP*SKFH RKLRRDLPLPVLALWRVGGRGYR*LPANVQQST GFHQVQPPQ*VLECSAGEGLC*APWFLRSSERWE HHRGHGGLHRRGGRVF*DQGCS**HVQDHEESH RERLPHGLLH**WHEHDLWNLSFWSEHGGVDCT DGKEYG*LTAPGLRPRPQRLR*KTDPDNHSGSV* DKNGLRAGQRSRLLCHGAG*GPVQR*ESEAGAA AESVGPGGVERFLE**MEGLELCGQR*EGPSAA PGH*GWRVLDVL*GFHLPFHKVGDLQPHGRCSAV *QASDLDSVCERGPLGTGLLCRRLPQLPRYFLD QPSVPSEAPGGGR*P**LGGDLQLPGGPDAEEP AEGPEARGQSLHHWLRHLRGSQRDAREQAAPAEG LLPVQRLQGQEQNLHQHAGGVPALPPASQRVRH RALHLRAPPGGGIHPPGLL*KEEPL*GS*KYHL RGSASAHHLRFGQSKQQQGAGCGPGVRGGQRQNK P**AKAVPTATAWQL*SGK*GTATIPEHFQADS RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLAE NFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS FSCI*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQ SHAGSLRISVSPSISKAIYLKGPSSYTPTGFQA PHQSCSSSILPPTHP*SVMPSLTL**SNEVGRT NPCPFAMWRKVPASGPSRLGSEASAPAYLALGCL QKHLPVALSTSLC*SPPSPSRCPTMGQEPNQHW VLLLWGKLTQWNRAGYFGLSNS*VWLHFEKS*S K*RHVYGW

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS WQISLKTQTTQQKNEICENPRFIIDGANRTDIC QGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFI ENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQL VFTKSNHRNEFWSALLEKAYAKLHGSYEALKGG NTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAI ERGSLMGCSIDDGTNMTYGTSPSGLNMGELIAR MVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQY ETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRL RNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQH QVTEDGEFWMSYEDFIYHFTKLEICNLTADALQ SDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWT NPQYRLKLLEEDDDPDDSEVICSFLVALMQKNR RKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQK DFFLYNASKARSKTYINMREVSQRFRLPPSEYVI VPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS VDRPVPIIFVSDRANSNKELGVDQESEEGKGKT SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIA GDDMEICADELKKVLNTVVNKHKDLKTHGFTLE SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQL YDIITMRYADKHMNIDFDSFICCFVRLEGMFRA FHAFDKDGDGIIKLNVLEWLQLTMYA*TRLASS KAMQDHSGFQFHPLFPKPFTSKDPAATPLQASRH LISHAPPPFYPLPILDRSCLA*PFSKAMR*EEQ TLVPLPCGGKCLPLVRAASVLKRVLLLTLL*AV CRSTCRWHSAPPCARALHHLHAVPPWARNQTSTG FYCCGVN*LSGIGLVTLGCPTHKFGCILKKADL NKGMCMAG

Sequence CAPN3.cAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC CATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACTCACTG CTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCA GAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTG GAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGG ATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGT CTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCT GGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGT CTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGG CCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCA GGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCATCTTC GTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCAC AGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGA TGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATG GATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACC AGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGC AGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGAT GGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTC AGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATT TTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGA AAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAG CACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACT CAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV WKRPPEICENPRFIIDGANRTDICQGELGDCWF LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS IDDGTNMTYGTSPSGLNMGELIARMVRNMDNSL LQDSDLDPRGSDERPTRTIIPVQYETRMACGLV RGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEW NGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFW MSYEDFIYHFTKLEICNLTADALQSDKLQTWTV SVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLL EEDDDPDDSEVICSFLVALMQKNRRKDRKLGAS LFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASK ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQ EGEFILRVFSEKRNLSEEVENTISVDRPVPIIF VSDRANSNKELGVDQESEEGKGKTSPDKQKQSP QPQPGSSDQESEEQQQFRNIFKQIAGDDMEICAD ELKKVLNTVVNKHKDLKTHGFTLESCRSMIALM DTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTD QSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYA DKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGD GIIKLNVLEWLQLTMYA*TRLASSKAMQDHSGF QFHPLFPKPFTSKDPAATPLQASRHLISHAPPPF YPLPILDRSCLA*PFSKAMR*EEQTLVPLPCGG KCLPLVRAASVLKRVLLLTLL*AVCRSTCRWHS APPCARALHHLHAVPPWARNQTSTGFYCCGVN*L SGIGLVTLGCPTHKFGCILKKADLNKGMCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA PLMMART*PMEPLLLV*TWGS*LHGW*GIWITHC SRTQTSTPEAQMKDRPGQSFRFSMRQEWPAGWS EVTPTLSRGWMRSRSKVRK*SWCGCGIRGARWS GTVLGVIDGRTGALWTKMRRPVCSTRSLRMESSG CPMRISSTISQSWRSATSRPMLCSLTSFRPGQC L*TRAAGYGVALPEAAATSQILSGPTLSTV*SS WRRTMTLMTRR*FAASWWP*CRRTGGRTGS*GPV SSPLASPSTRFPKRCTGTSSTCRRTSSCTTPPR PGAKPTSTCGRCPSASACLPASTSSCPPPTSPT RRGNSSSGSSLKRGTSLRKLKIPSPWIGQCPSSS FRTEQTATRSWVWTRSQRRAKAKQALISKSSPH SHSLAALIRKVRNSNNSGTFSSR*QEMTWRSVQ MSSRRSLTQS*TNTRT*RHTGSHWSPAVA*LRSW IQMALESSTCRSSTTSGTRLRPGRKFSNTMTQT SPAPSTATRCEMQSTTQDSTSTTSSMTSLPCGT QTNT*TSTLTVSSAASLGWRACSELFMHLTRMEM VSSSSTFWSGCSSPCMPEPGWPHPKPCRITQDF SFTLYFQSHLPQRTQQLHPYRLPGTSSVMLLLH FTPYPSLIGHA*PDPLVKQ*GRKNKPLSLCHVEE SACLWSEPPRF*SECSCLPCSRLSAEAPAGGTQ HLLVLEPSITFTLSHHGPGTKPALGSTAVG*TN SVE*GWLLWAVQLISLAAF*KKLI*IKACVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH *GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ *VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL H**WHEHDLWNLSFWSEHGGVDCTDGKEYG*LT APGLRPRPQRLR*KTDPDNHSGSV*DKNGLRAGQ RSRLLCHGAG*GPVQR*ESEAGAAAESVGPGGV ERFLE**MEGLELCGQR*EGPSAAPGH*GWRVL DVL*GFHLPFHKVGDLQPHGRCSAV*QASDLDSV CERGPLGTGLLCRRLPQLPRYFLDQPSVPSEAP GGGR*P**LGGDLQLPGGPDAEEPAEGPEARGQ SLHHWLRHLRGSQRDAREQAAPAEGLLPVQRLQG QEQNLHQHAGGVPALPPASQRVRHRALHLRAPP GGGIHPPGLL*KEEPL*GS*KYHLRGSASAHHL RFGQSKQQQGAGCGPGVRGGQRQNKP**AKAVPT ATAWQL*SGK*GTATIPEHFQADSRR*HGDLCR *AQEGP*HSREQTQGPEDTRVHTGVLP*HDCAH GYRWLWKAQPAGVPPPLEQD*GLAENFQTL*HRP VRHHQQLRDAKCSQRRRIPPQQPAL*HHYHAVR RQTHEHRL*QFHLLLR*AGGHVQSFSCI*QGWR WYHQAQRSGVAAAHHVCLNQAGLIQSHAGSLRIS VSPSISKAIYLKGPSSYTPTGFQAPHQSCSSSI LPPTHP*SVMPSLTL**SNEVGRTNPCPFAMWR KVPASGPSRLGSEASAPAYLALGCLQKHLPVALS TSLC*SPPSPSRCPTMGQEPNQHWVLLLWGKLT QWNRAGYFGLSNS*VWLHFEKS*SK*RHVYGW

Sequence CAPN3.jAug05

CCCTCCTCCACGCTTACAGCCACACACACAGTCACACAGACGCGTTCTGAGGGTGGCTGCCCGCTTGGGATGGAGGAATCACTTCCCTCAGAACCCAGCC AAGTCCTCTAGGCCTCCTTGGGGGTCCTTCCAGCCTGAGGGGCTTCGGAGCTGAGGAGCAGCTGTTCTGATGCACGGGAACAAGCAGCACCTGCAGAAGG ACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCAT CGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCC GTGGATCGGCCAGTGAAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGG AGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAA CATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACA CACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACA AGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATT CCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTG GAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAA CCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACC CCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCA ATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTA CCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAG GAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATT TTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PSSTLTATHTVTQTRSEGGCPLGMEESLPSEPS QVL*ASLGVLPA*GASELRSSCSDAREQAAPAEG LLPVQRLQGQEQNLHQHAGGVPALPPASQRVRH RALHLRAPPGGGIHPPGLL*KEEPL*GS*KYHL RGSASEKEKNQAHHLRFGQSKQQQGAGCGPGVRG GQRQNKP**AKAVPTATAWQL*SGK*GTATIPE HFQADSRR*HGDLCR*AQEGP*HSREQTQGPED TRVHTGVLP*HDCAHGYRWLWKAQPAGVPPPLEQ D*GLAENFQTL*HRPVRHHQQLRDAKCSQRRRI PPQQPAL*HHYHAVRRQTHEHRL*QFHLLLR*A GGHVQSFSCI*QGWRWYHQAQRSGVAAAHHVCLN QAGLIQSHAGSLRISVSPSISKAIYLKGPSSYT PTGFQAPHQSCSSSILPPTHP*SVMPSLTL**S NEVGRTNPCPFAMWRKVPASGPSRLGSEASAPAY LALGCLQKHLPVALSTSLC*SPPSPSRCPTMGQ EPNQHWVLLLWGKLTQWNRAGYFGLSNS*VWLH FEKS*SK*RHVYGW

Frame 1

PPPRLQPHTQSHRRVLRVAARLGWRNHFPQNPA KSSRPPWGSFQPEGLRS*GAAVLMHGNKQHLQK DFFLYNASKARSKTYINMREVSQRFRLPPSEYVI VPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS VDRPVKKKKTKPIIFVSDRANSNKELGVDQESE EGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRN IFKQIAGDDMEICADELKKVLNTVVNKHKDLKT HGFTLESCRSMIALMDTDGSGKLNLQEFHHLWN KIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGF HLNNQLYDIITMRYADKHMNIDFDSFICCFVRL EGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA* TRLASSKAMQDHSGFQFHPLFPKPFTSKDPAATP LQASRHLISHAPPPFYPLPILDRSCLA*PFSKA MR*EEQTLVPLPCGGKCLPLVRAASVLKRVLLL TLL*AVCRSTCRWHSAPPCARALHHLHAVPPWAR NQTSTGFYCCGVN*LSGIGLVTLGCPTHKFGCI LKKADLNKGMCMAG

Frame 2

LLHAYSHTHSHTDAF*GWLPAWDGGITSLRTQP SPLGLLGGPSSLRGFGAEEQLF*CTGTSSTCRR TSSCTTPPRPGAKPTSTCGRCPSASACLPASTS SCPPPTSPTRRGNSSSGSSLKRGTSLRKLKIPSP WIGQ*KRKKPSPSSSFRTEQTATRSWVWTRSQR RAKAKQALISKSSPHSHSLAALIRKVRNSNNSG TFSSR*QEMTWRSVQMSSRRSLTQS*TNTRT*RH TGSHWSPAVA*LRSWIQMALESSTCRSSTTSGT RLRPGRKFSNTMTQTSPAPSTATRCEMQSTTQD STSTTSSMTSLPCGTQTNT*TSTLTVSSAASLGW RACSELFMHLTRMEMVSSSSTFWSGCSSPCMPE PGWPHPKPCRITQDFSFTLYFQSHLPQRTQQLH PYRLPGTSSVMLLLHFTPYPSLIGHA*PDPLVKQ *GRKNKPLSLCHVEESACLWSEPPRF*SECSCL PCSRLSAEAPAGGTQHLLVLEPSITFTLSHHGP GTKPALGSTAVG*TNSVE*GWLLWAVQLISLAAF *KKLI*IKACVWLV

Sequence CAPN3.iAug05

CCCCATCTCTCAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGG GAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCT CTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGAAAAAGAAAAAAACCAAGCCCATCATCTTCGTTTCGGACAG AGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCT GGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGA AGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGG CTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACC ATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACA TGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAA GCTCAACGTTCTGGAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGG GCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCT GCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

PHLSDAREQAAPAEGLLPVQRLQGQEQNLHQHA GGVPALPPASQRVRHRALHLRAPPGGGIHPPGLL *KEEPL*GS*KYHLRGSASEKEKNQAHHLRFGQ SKQQQGAGCGPGVRGGQRQNKP**AKAVPTATA WQL*SGK*GTATIPEHFQADSRR*HGDLCR*AQE GP*HSREQTQGPEDTRVHTGVLP*HDCAHGYRW LWKAQPAGVPPPLEQD*GLAENFQTL*HRPVRH HQQLRDAKCSQRRRIPPQQPAL*HHYHAVRRQTH EHRL*QFHLLLR*AGGHVQSFSCI*QGWRWYHQ AQRSGGCLQKHLPVALSTSLC*SPPSPSRCPTM GQEPNQHWVLLLWGKLTQWNRAGYFGLSNS*VWL HFEKS*SK*RHVYGW

Frame 1

PISQMHGNKQHLQKDFFLYNASKARSKTYINMR EVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVF SEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDR ANSNKELGVDQESEEGKGKTSPDKQKQSPQPQP GSSDQESEEQQQFRNIFKQIAGDDMEICADELK KVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDG SGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGT INSYEMRNAVNDAGFHLNNQLYDIITMRYADKH MNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIK LNVLEAVCRSTCRWHSAPPCARALHHLHAVPPW ARNQTSTGFYCCGVN*LSGIGLVTLGCPTHKFG CILKKADLNKGMCMAG

Frame 2

PSLRCTGTSSTCRRTSSCTTPPRPGAKPTSTCG RCPSASACLPASTSSCPPPTSPTRRGNSSSGSS LKRGTSLRKLKIPSPWIGQ*KRKKPSPSSSFRT EQTATRSWVWTRSQRRAKAKQALISKSSPHSHSL AALIRKVRNSNNSGTFSSR*QEMTWRSVQMSSR RSLTQS*TNTRT*RHTGSHWSPAVA*LRSWIQM ALESSTCRSSTTSGTRLRPGRKFSNTMTQTSPAP STATRCEMQSTTQDSTSTTSSMTSLPCGTQTNT *TSTLTVSSAASLGWRACSELFMHLTRMEMVSS SSTFWRLSAEAPAGGTQHLLVLEPSITFTLSHHG PGTKPALGSTAVG*TNSVE*GWLLWAVQLISLA AF*KKLI*IKACVWLV

Sequence CAPN3.bAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC CATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACTCACTG CTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCA GAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTG GAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGG ATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGT CTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCT GGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGT CTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGG CCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCA GGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGAAAAAGAAAAAA ACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATA AGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGA CATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGT AGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCA AACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACAT CATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCA TTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGC AGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAG TCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTC CCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCA CCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTG CTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGC ATGTGTATGGCTGGTC

CAPN3.u.f.52 (forward primer)
CAPN3.u.r.55 (reverse primer)
Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV WKRPPEICENPRFIIDGANRTDICQGELGDCWF LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS IDDGTNMTYGTSPSGLNMGELIARMVRNMDNSL LQDSDLDPRGSDERPTRTIIPVQYETRMACGLV RGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEW NGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFW MSYEDFIYHFTKLEICNLTADALQSDKLQTWTV SVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLL EEDDDPDDSEVICSFLVALMQKNRRKDRKLGAS LFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASK ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQ EGEFILRVFSEKRNLSEEVENTISVDRPVKKKK TKPIIFVSDRANSNKELGVDQESEEGKGKTSPD KQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDD MEICADELKKVLNTVVNKHKDLKTHGFTLESCR SMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIF KHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDI ITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA FDKDGDGIIKLNVLEWLQLTMYA*TRLASSKAM QDHSGFQFHPLFPKPFTSKDPAATPLQASRHLIS HAPPPFYPLPILDRSCLA*PFSKAMR*EEQTLV PLPCGGKCLPLVRAASVLKRVLLLTLL*AVCRS TCRWHSAPPCARALHHLHAVPPWARNQTSTGFYC CGVN*LSGIGLVTLGCPTHKFGCILKKADLNKG MCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA PLMMART*PMEPLLLV*TWGS*LHGW*GIWITHC SRTQTSTPEAQMKDRPGQSFRFSMRQEWPAGWS EVTPTLSRGWMRSRSKVRK*SWCGCGIRGARWS GTVLGVIDGRTGALWTKMRRPVCSTRSLRMESSG CPMRISSTISQSWRSATSRPMLCSLTSFRPGQC L*TRAAGYGVALPEAAATSQILSGPTLSTV*SS WRRTMTLMTRR*FAASWWP*CRRTGGRTGS*GPV SSPLASPSTRFPKRCTGTSSTCRRTSSCTTPPR PGAKPTSTCGRCPSASACLPASTSSCPPPTSPT RRGNSSSGSSLKRGTSLRKLKIPSPWIGQ*KRKK PSPSSSFRTEQTATRSWVWTRSQRRAKAKQALI SKSSPHSHSLAALIRKVRNSNNSGTFSSR*QEM TWRSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAV A*LRSWIQMALESSTCRSSTTSGTRLRPGRKFS NTMTQTSPAPSTATRCEMQSTTQDSTSTTSSMT SLPCGTQTNT*TSTLTVSSAASLGWRACSELFMH LTRMEMVSSSSTFWSGCSSPCMPEPGWPHPKPC RITQDFSFTLYFQSHLPQRTQQLHPYRLPGTSS VMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNKPLS LCHVEESACLWSEPPRF*SECSCLPCSRLSAEA PAGGTQHLLVLEPSITFTLSHHGPGTKPALGST AVG*TNSVE*GWLLWAVQLISLAAF*KKLI*IKA CVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH *GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ *VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL H**WHEHDLWNLSFWSEHGGVDCTDGKEYG*LT APGLRPRPQRLR*KTDPDNHSGSV*DKNGLRAGQ RSRLLCHGAG*GPVQR*ESEAGAAAESVGPGGV ERFLE**MEGLELCGQR*EGPSAAPGH*GWRVL DVL*GFHLPFHKVGDLQPHGRCSAV*QASDLDSV CERGPLGTGLLCRRLPQLPRYFLDQPSVPSEAP GGGR*P**LGGDLQLPGGPDAEEPAEGPEARGQ SLHHWLRHLRGSQRDAREQAAPAEGLLPVQRLQG QEQNLHQHAGGVPALPPASQRVRHRALHLRAPP GGGIHPPGLL*KEEPL*GS*KYHLRGSASEKEK NQAHHLRFGQSKQQQGAGCGPGVRGGQRQNKP** AKAVPTATAWQL*SGK*GTATIPEHFQADSRR* HGDLCR*AQEGP*HSREQTQGPEDTRVHTGVLP *HDCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQ TL*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*H HYHAVRRQTHEHRL*QFHLLLR*AGGHVQSFSC I*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQSHA GSLRISVSPSISKAIYLKGPSSYTPTGFQAPHQ SCSSSILPPTHP*SVMPSLTL**SNEVGRTNPC PFAMWRKVPASGPSRLGSEASAPAYLALGCLQKH LPVALSTSLC*SPPSPSRCPTMGQEPNQHWVLL LWGKLTQWNRAGYFGLSNS*VWLHFEKS*SK*R HVYGW