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Sequences

Sequence CAPN3.dAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC
TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT
CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA
CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA
CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA
CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC
TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC
TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT
CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT
GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA
CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC
CATTGATACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTC
AAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGG
ACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGAT
CTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGC
TGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCT
TCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGAT
GCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGC
TTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACC
TCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACAT
TTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACAC
GGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGA
TTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCA
CCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAG
GGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCA
GGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCT
ACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATG
AGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCT
TGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAA
CCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTG
AAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE
LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL
R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP
LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA
TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH
KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV
WKRPPEICENPRFIIDGANRTDICQGELGDCWF
LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF
QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN
EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF
TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS
IDTIIPVQYETRMACGLVRGHAYSVTGLDEVPF
KGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFV
DKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEI
CNLTADALQSDKLQTWTVSVNEGRWVRGCSAGG
CRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICS
FLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEM
HGNKQHLQKDFFLYNASKARSKTYINMREVSQR
FRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRN
LSEEVENTISVDRPVPQPGSSDQESEEQQQFRNI
FKQIAGDDMEICADELKKVLNTVVNKHKDLKTH
GFTLESCRSMIALMDTDGSGKLNLQEFHHLWNK
IKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFH
LNNQLYDIITMRYADKHMNIDFDSFICCFVRLE
GMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA*T
RLASSKAMQDHSGFQFHPLFPKPFTSKDPAATPL
QASRHLISHAPPPFYPLPILDRSCLA*PFSKAM
R*EEQTLVPLPCGGKCLPLVRAASVLKRVLLLT
LL*AVCRSTCRWHSAPPCARALHHLHAVPPWARN
QTSTGFYCCGVN*LSGIGLVTLGCPTHKFGCIL
KKADLNKGMCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN
LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL
SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH
LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP
LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF
TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS
GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF
SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST
SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM
SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS
QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA
PLIQSFRFSMRQEWPAGWSEVTPTLSRGWMRSRS
KVRK*SWCGCGIRGARWSGTVLGVIDGRTGALW
TKMRRPVCSTRSLRMESSGCPMRISSTISQSWR
SATSRPMLCSLTSFRPGQCL*TRAAGYGVALPEA
AATSQILSGPTLSTV*SSWRRTMTLMTRR*FAA
SWWP*CRRTGGRTGS*GPVSSPLASPSTRFPKR
CTGTSSTCRRTSSCTTPPRPGAKPTSTCGRCPSA
SACLPASTSSCPPPTSPTRRGNSSSGSSLKRGT
SLRKLKIPSPWIGQCHSLAALIRKVRNSNNSGT
FSSR*QEMTWRSVQMSSRRSLTQS*TNTRT*RHT
GSHWSPAVA*LRSWIQMALESSTCRSSTTSGTR
LRPGRKFSNTMTQTSPAPSTATRCEMQSTTQDS
TSTTSSMTSLPCGTQTNT*TSTLTVSSAASLGWR
ACSELFMHLTRMEMVSSSSTFWSGCSSPCMPEP
GWPHPKPCRITQDFSFTLYFQSHLPQRTQQLHP
YRLPGTSSVMLLLHFTPYPSLIGHA*PDPLVKQ*
GRKNKPLSLCHVEESACLWSEPPRF*SECSCLP
CSRLSAEAPAGGTQHLLVLEPSITFTLSHHGPG
TKPALGSTAVG*TNSVE*GWLLWAVQLISLAAF*
KKLI*IKACVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT
YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS
QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA
TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH
*GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS
QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR
LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS
RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL
PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ
*VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH
RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL
H*YNHSGSV*DKNGLRAGQRSRLLCHGAG*GPV
QR*ESEAGAAAESVGPGGVERFLE**MEGLELCG
QR*EGPSAAPGH*GWRVLDVL*GFHLPFHKVGD
LQPHGRCSAV*QASDLDSVCERGPLGTGLLCRR
LPQLPRYFLDQPSVPSEAPGGGR*P**LGGDLQL
PGGPDAEEPAEGPEARGQSLHHWLRHLRGSQRD
AREQAAPAEGLLPVQRLQGQEQNLHQHAGGVPA
LPPASQRVRHRALHLRAPPGGGIHPPGLL*KEEP
L*GS*KYHLRGSASATAWQL*SGK*GTATIPEH
FQADSRR*HGDLCR*AQEGP*HSREQTQGPEDT
RVHTGVLP*HDCAHGYRWLWKAQPAGVPPPLEQD
*GLAENFQTL*HRPVRHHQQLRDAKCSQRRRIP
PQQPAL*HHYHAVRRQTHEHRL*QFHLLLR*AG
GHVQSFSCI*QGWRWYHQAQRSGVAAAHHVCLNQ
AGLIQSHAGSLRISVSPSISKAIYLKGPSSYTP
TGFQAPHQSCSSSILPPTHP*SVMPSLTL**SN
EVGRTNPCPFAMWRKVPASGPSRLGSEASAPAYL
ALGCLQKHLPVALSTSLC*SPPSPSRCPTMGQE
PNQHWVLLLWGKLTQWNRAGYFGLSNS*VWLHF
EKS*SK*RHVYGW

Sequence CAPN3.hAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT
TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT
TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA
AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT
CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT
TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC
AAGGAGAGCTAGGGGACTGCTGGTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCAT
CGAAAACTACGCAGGGATCTTCCACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTG
GTTTTCACCAAGTCCAACCACCGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGA
ACACCACAGAGGCCATGGAGGACTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCAT
CGAGAGAGGCTCCCTCATGGGCTGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGG
ATGGTAAGGAATATGGATAACTCACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATG
AGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCT
GCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCAC
CAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGT
CTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGAC
CAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGG
CGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGGTATAGCAGCAGCAGCGGCCAGCAGTTGTGT
GCAGCACTACCCAGGGGGCCCCGAGTCTGTCTGTGGCTCGTCGAGAAGCTTCCTGGTGGGGTTTGTGGGCAGGACTGTGATAGGAGAGGGCCTTGCCTGT
GTTATTTCCACTGCAGAGCAGGTGCCTCAGGGCATTGCATGACCCATGACTACCACCCCCAGGATGTGCACTTTCTCCCTCGCACCAGACACTGCACGTC
ACACACATGCCTTTGCACACTCACCCTCCTCCACGCTTACAGCCACACACACAGTCACACAGACGCGTTCTGAGGGTGGCTGCCCGCTTGGGATGGAGGA
ATCACTTCCCTCAGAACCCAGCCAAGTCCTCTAGGCCTCCTTGGGGGTCCTTCCAGCCTGAGGGGCTTCGGAGCTGAGGAGCAGCTGTTCTGGTAAGTGT
CCCTGAGTGTGGGGATGACACATTTGCCATTCACTCTGAATCACAACAGAAAAGGAAGAGGAAGTGAGGTAGGGAGGCTATTTAAGCCTTGGGAGTCGGA
AGTAGGGAGGTTGAAACTGTGACATGGGTGACCAGGGAGTTGGGAAGGGACCCTTGGAGGTGGCTGTGGCAGGACAGGATGTTCCTCCCGAGGGGCTCAT
GTGCCCTGGGCTCTCCCCATCTCTCAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCT
ACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCAT
CCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCATCTTCGTTTCGGACAGAGCA
AACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCA
GCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGT
CCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCT
GGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCA
ACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAA
CATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTC
AACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTAT
TTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCAT
CCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTG
GTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTA
GAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCT
GGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG
LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF
HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG
RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS
SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V
GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV
KES*GTAGFSQPLPA*P*TSTFFSESYPMIKVS
SKTTQGSSTSSSGAMESGWTWL*MTACQRTTINW
FSPSPTTAMSSGVLCWRRLMLSSMVPTKL*KVG
TPQRPWRTSQEGWQSFLRSGMLLVTCTRS*RKP
SREAPSWAAPLMMART*PMEPLLLV*TWGS*LHG
W*GIWITHCSRTQTSTPEAQMKDRPGQSFRFSM
RQEWPAGWSEVTPTLSRGWMRSRSKVRK*SWCG
CGIRGARWSGTVLGVIDGRTGALWTKMRRPVCST
RSLRMESSGCPMRISSTISQSWRSATSRPMLCS
LTSFRPGQCL*TRAAGYGVALPEAAATSQILSG
PTLSTV*SSWRRTMTLMTRR*FAASWWP*CRRTG
GRTGS*GPVSSPLASPSTRFPKRYSSSSGQQLC
AALPRGPRVCLWLVEKLPGGVCGQDCDRRGPCL
CYFHCRAGASGHCMTHDYHPQDVHFLPRTRHCTS
HTCLCTLTLLHAYSHTHSHTDAF*GWLPAWDGG
ITSLRTQPSPLGLLGGPSSLRGFGAEEQLFW*V
SLSVGMTHLPFTLNHNRKGRGSEVGRLFKPWESE
VGRLKL*HG*PGSWEGTLGGGCGRTGCSSRGAH
VPWALPISQMHGNKQHLQKDFFLYNASKARSKT
YINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFI
LRVFSEKRNLSEEVENTISVDRPVPIIFVSDRA
NSNKELGVDQESEEGKGKTSPDKQKQSPQPQPG
SSDQESEEQQQFRNIFKQIAGDDMEICADELKKV
LNTVVNKHKDLKTHGFTLESCRSMIALMDTDGS
GKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTI
NSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMN
IDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKL
NVLEWLQLTMYA*TRLASSKAMQDHSGFQFHPL
FPKPFTSKDPAATPLQASRHLISHAPPPFYPLPI
LDRSCLA*PFSKAMR*EEQTLVPLPCGGKCLPL
VRAASVLKRVLLLTLL*AVCRSTCRWHSAPPCA
RALHHLHAVPPWARNQTSTGFYCCGVN*LSGIGL
VTLGCPTHKFGCILKKADLNKGMCMAG

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG
CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV
FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE
GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA
LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE
LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS
RRARGLLVSRSHCLPDPEPAPSFPSHTP*SKFH
RKLRRDLPLPVLALWRVGGRGYR*LPANVQQST
GFHQVQPPQ*VLECSAGEGLC*APWFLRSSERWE
HHRGHGGLHRRGGRVF*DQGCS**HVQDHEESH
RERLPHGLLH**WHEHDLWNLSFWSEHGGVDCT
DGKEYG*LTAPGLRPRPQRLR*KTDPDNHSGSV*
DKNGLRAGQRSRLLCHGAG*GPVQR*ESEAGAA
AESVGPGGVERFLE**MEGLELCGQR*EGPSAA
PGH*GWRVLDVL*GFHLPFHKVGDLQPHGRCSAV
*QASDLDSVCERGPLGTGLLCRRLPQLPRYFLD
QPSVPSEAPGGGR*P**LGGDLQLPGGPDAEEP
AEGPEARGQSLHHWLRHLRGSQRGIAAAAASSCV
QHYPGGPESVCGSSRSFLVGFVGRTVIGEGLAC
VISTAEQVPQGIA*PMTTTPRMCTFSLAPDTAR
HTHAFAHSPSSTLTATHTVTQTRSEGGCPLGMEE
SLPSEPSQVL*ASLGVLPA*GASELRSSCSGKC
P*VWG*HICHSL*ITTEKEEEVR*GGYLSLGSR
K*GG*NCDMGDQGVGKGPLEVAVAGQDVPPEGLM
CPGLSPSLRCTGTSSTCRRTSSCTTPPRPGAKP
TSTCGRCPSASACLPASTSSCPPPTSPTRRGNS
SSGSSLKRGTSLRKLKIPSPWIGQCPSSSFRTEQ
TATRSWVWTRSQRRAKAKQALISKSSPHSHSLA
ALIRKVRNSNNSGTFSSR*QEMTWRSVQMSSRR
SLTQS*TNTRT*RHTGSHWSPAVA*LRSWIQMAL
ESSTCRSSTTSGTRLRPGRKFSNTMTQTSPAPS
TATRCEMQSTTQDSTSTTSSMTSLPCGTQTNT*
TSTLTVSSAASLGWRACSELFMHLTRMEMVSSSS
TFWSGCSSPCMPEPGWPHPKPCRITQDFSFTLY
FQSHLPQRTQQLHPYRLPGTSSVMLLLHFTPYP
SLIGHA*PDPLVKQ*GRKNKPLSLCHVEESACLW
SEPPRF*SECSCLPCSRLSAEAPAGGTQHLLVL
EPSITFTLSHHGPGTKPALGSTAVG*TNSVE*G
WLLWAVQLISLAAF*KKLI*IKACVWLV

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV
ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF
SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR
KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL
SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS
WQISLKTQTTQQKNEICENPRFIIDGANRTDIC
QGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFI
ENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQL
VFTKSNHRNEFWSALLEKAYAKLHGSYEALKGG
NTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAI
ERGSLMGCSIDDGTNMTYGTSPSGLNMGELIAR
MVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQY
ETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRL
RNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQH
QVTEDGEFWMSYEDFIYHFTKLEICNLTADALQ
SDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWT
NPQYRLKLLEEDDDPDDSEVICSFLVALMQKNR
RKDRKLGASLFTIGFAIYEVPKEV*QQQRPAVV
CSTTQGAPSLSVARREASWWGLWAGL**ERALPV
LFPLQSRCLRALHDP*LPPPGCALSPSHQTLHV
THMPLHTHPPPRLQPHTQSHRRVLRVAARLGWR
NHFPQNPAKSSRPPWGSFQPEGLRS*GAAVLVSV
PECGDDTFAIHSESQQKRKRK*GREAI*ALGVG
SREVETVTWVTRELGRDPWRWLWQDRMFLPRGS
CALGSPHLSDAREQAAPAEGLLPVQRLQGQEQNL
HQHAGGVPALPPASQRVRHRALHLRAPPGGGIH
PPGLL*KEEPL*GS*KYHLRGSASAHHLRFGQS
KQQQGAGCGPGVRGGQRQNKP**AKAVPTATAWQ
L*SGK*GTATIPEHFQADSRR*HGDLCR*AQEG
P*HSREQTQGPEDTRVHTGVLP*HDCAHGYRWL
WKAQPAGVPPPLEQD*GLAENFQTL*HRPVRHHQ
QLRDAKCSQRRRIPPQQPAL*HHYHAVRRQTHE
HRL*QFHLLLR*AGGHVQSFSCI*QGWRWYHQA
QRSGVAAAHHVCLNQAGLIQSHAGSLRISVSPSI
SKAIYLKGPSSYTPTGFQAPHQSCSSSILPPTH
P*SVMPSLTL**SNEVGRTNPCPFAMWRKVPAS
GPSRLGSEASAPAYLALGCLQKHLPVALSTSLC*
SPPSPSRCPTMGQEPNQHWVLLLWGKLTQWNRA
GYFGLSNS*VWLHFEKS*SK*RHVYGW

Sequence CAPN3.fAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT
TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT
TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA
AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT
CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT
TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC
AAGGAGAGCTAGACGGGTTCCAGGGCAGCTGCCTACCTGGCCTTCCTTCCAATACAAATCATCTTGGTGGATGGTTCTCTGAGGCTCAGTCTTCGCTGAA
GTCAGAAGAGGAATTGGACTCACATTGCAAAGGCACAGGGCAGGGCAGATTTCCTACAGGTGTTAGGAAGAACAACCCAGTTATGATCACCTACTGCTCT
GTCTCCATTGAGGCCTAAAAAGGAAGTGAGTTTATACTGCAGTTGGAGGAACTGCCTGCAGCCTTGAGGAAAATGTCTAGTCACAAGGGAGGGACTGCTG
GTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTC
CACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACC
GCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGA
CTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGC
TGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACT
CACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCT
GGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTG
GAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGT
TCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGAC
AGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAG
CTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGG
CCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTC
CAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCC
CACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCA
TCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTC
CCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGT
GCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGC
TCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACAC
AGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGG
TACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATG
GAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGG
ATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCT
CCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTG
GAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCA
CTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAAC
TAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTG
GTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG
LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF
HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG
RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS
SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V
GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV
KES*TGSRAAAYLAFLPIQIILVDGSLRLSLR*
SQKRNWTHIAKAQGRADFLQVLGRTTQL*SPTAL
SPLRPKKEVSLYCSWRNCLQP*GKCLVTREGLL
VSRSHCLPDPEPAPSFPSHTP*SKFHRKLRRDL
PLPVLALWRVGGRGYR*LPANVQQSTGFHQVQPP
Q*VLECSAGEGLC*APWFLRSSERWEHHRGHGG
LHRRGGRVF*DQGCS**HVQDHEESHRERLPHG
LLH**WHEHDLWNLSFWSEHGGVDCTDGKEYG*L
TAPGLRPRPQRLR*KTDPDNHSGSV*DKNGLRA
GQRSRLLCHGAG*GPVQR*ESEAGAAAESVGPG
GVERFLE**MEGLELCGQR*EGPSAAPGH*GWRV
LDVL*GFHLPFHKVGDLQPHGRCSAV*QASDLD
SVCERGPLGTGLLCRRLPQLPRYFLDQPSVPSE
APGGGR*P**LGGDLQLPGGPDAEEPAEGPEARG
QSLHHWLRHLRGSQRDAREQAAPAEGLLPVQRL
QGQEQNLHQHAGGVPALPPASQRVRHRALHLRA
PPGGGIHPPGLL*KEEPL*GS*KYHLRGSASAHH
LRFGQSKQQQGAGCGPGVRGGQRQNKP**AKAV
PTATAWQL*SGK*GTATIPEHFQADSRR*HGDL
CR*AQEGP*HSREQTQGPEDTRVHTGVLP*HDCA
HGYRWLWKAQPAGVPPPLEQD*GLAENFQTL*H
RPVRHHQQLRDAKCSQRRRIPPQQPAL*HHYHA
VRRQTHEHRL*QFHLLLR*AGGHVQSFSCI*QGW
RWYHQAQRSGVAAAHHVCLNQAGLIQSHAGSLR
ISVSPSISKAIYLKGPSSYTPTGFQAPHQSCSS
SILPPTHP*SVMPSLTL**SNEVGRTNPCPFAMW
RKVPASGPSRLGSEASAPAYLALGCLQKHLPVA
LSTSLC*SPPSPSRCPTMGQEPNQHWVLLLWGK
LTQWNRAGYFGLSNS*VWLHFEKS*SK*RHVYGW

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG
CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV
FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE
GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA
LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE
LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS
RRARRVPGQLPTWPSFQYKSSWWMVL*GSVFAE
VRRGIGLTLQRHRAGQISYRC*EEQPSYDHLLL
CLH*GLKRK*VYTAVGGTACSLEENV*SQGRDCW
FLAAIACLTLNQHLLFRVIPHDQSFIENYAGIF
HFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNH
RNEFWSALLEKAYAKLHGSYEALKGGNTTEAMED
FTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMG
CSIDDGTNMTYGTSPSGLNMGELIARMVRNMDN
SLLQDSDLDPRGSDERPTRTIIPVQYETRMACGL
VRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQV
EWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGE
FWMSYEDFIYHFTKLEICNLTADALQSDKLQTWT
VSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLK
LLEEDDDPDDSEVICSFLVALMQKNRRKDRKLG
ASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNAS
KARSKTYINMREVSQRFRLPPSEYVIVPSTYEP
HQEGEFILRVFSEKRNLSEEVENTISVDRPVPI
IFVSDRANSNKELGVDQESEEGKGKTSPDKQKQS
PQPQPGSSDQESEEQQQFRNIFKQIAGDDMEIC
ADELKKVLNTVVNKHKDLKTHGFTLESCRSMIA
LMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDT
DQSGTINSYEMRNAVNDAGFHLNNQLYDIITMR
YADKHMNIDFDSFICCFVRLEGMFRAFHAFDKD
GDGIIKLNVLEWLQLTMYA*TRLASSKAMQDHSG
FQFHPLFPKPFTSKDPAATPLQASRHLISHAPP
PFYPLPILDRSCLA*PFSKAMR*EEQTLVPLPC
GGKCLPLVRAASVLKRVLLLTLL*AVCRSTCRWH
SAPPCARALHHLHAVPPWARNQTSTGFYCCGVN
*LSGIGLVTLGCPTHKFGCILKKADLNKGMCMA
G

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV
ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF
SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR
KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL
SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS
WQISLKTQTTQQKNEICENPRFIIDGANRTDIC
QGELDGFQGSCLPGLPSNTNHLGGWFSEAQSSLK
SEEELDSHCKGTGQGRFPTGVRKNNPVMITYCS
VSIEA*KGSEFILQLEELPAALRKMSSHKGGTA
GFSQPLPA*P*TSTFFSESYPMIKVSSKTTQGSS
TSSSGAMESGWTWL*MTACQRTTINWFSPSPTT
AMSSGVLCWRRLMLSSMVPTKL*KVGTPQRPWR
TSQEGWQSFLRSGMLLVTCTRS*RKPSREAPSWA
APLMMART*PMEPLLLV*TWGS*LHGW*GIWIT
HCSRTQTSTPEAQMKDRPGQSFRFSMRQEWPAG
WSEVTPTLSRGWMRSRSKVRK*SWCGCGIRGARW
SGTVLGVIDGRTGALWTKMRRPVCSTRSLRMES
SGCPMRISSTISQSWRSATSRPMLCSLTSFRPG
QCL*TRAAGYGVALPEAAATSQILSGPTLSTV*S
SWRRTMTLMTRR*FAASWWP*CRRTGGRTGS*G
PVSSPLASPSTRFPKRCTGTSSTCRRTSSCTTP
PRPGAKPTSTCGRCPSASACLPASTSSCPPPTSP
TRRGNSSSGSSLKRGTSLRKLKIPSPWIGQCPS
SSFRTEQTATRSWVWTRSQRRAKAKQALISKSS
PHSHSLAALIRKVRNSNNSGTFSSR*QEMTWRSV
QMSSRRSLTQS*TNTRT*RHTGSHWSPAVA*LR
SWIQMALESSTCRSSTTSGTRLRPGRKFSNTMT
QTSPAPSTATRCEMQSTTQDSTSTTSSMTSLPCG
TQTNT*TSTLTVSSAASLGWRACSELFMHLTRM
EMVSSSSTFWSGCSSPCMPEPGWPHPKPCRITQ
DFSFTLYFQSHLPQRTQQLHPYRLPGTSSVMLLL
HFTPYPSLIGHA*PDPLVKQ*GRKNKPLSLCHV
EESACLWSEPPRF*SECSCLPCSRLSAEAPAGG
TQHLLVLEPSITFTLSHHGPGTKPALGSTAVG*T
NSVE*GWLLWAVQLISLAAF*KKLI*IKACVWL
V

Sequence CAPN3.eAug05

CTATTCCAGTGTTTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATCCTCCTATGGACTCCGGGT
TGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGCCATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTT
TCATTCTGTTCCAGTGTTCTGATCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGGCTTCAGGA
AGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACGTATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCT
CTCACTCAACATTGCCACTGACTGGGAGGGTTCCTAAAAAAGCTCTAAAATGAATGCATAGTGTGTCTTTCCTTCAAGATGTAAATGTCAAGGAATGAGT
TGGCAAATCAGTTTAAAAACACAAACTACCCAACAGAAAAATGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTC
AAGGAGAGCTAGGGGACTGCTGGTTTCTCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCAT
CGAAAACTACGCAGGGATCTTCCACTTCCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTG
GTTTTCACCAAGTCCAACCACCGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGA
ACACCACAGAGGCCATGGAGGACTTCACAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCAT
CGAGAGAGGCTCCCTCATGGGCTGCTCCATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGG
ATGGTAAGGAATATGGATAACTCACTGCTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATG
AGACAAGAATGGCCTGCGGGCTGGTCAGAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCT
GCGGAATCCGTGGGGCCAGGTGGAGTGGAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCAC
CAGGTCACTGAGGATGGAGAGTTCTGGATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGT
CTGACAAGCTTCAGACCTGGACAGTGTCTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGAC
CAACCCTCAGTACCGTCTGAAGCTCCTGGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGG
CGGAAGGACCGGAAGCTAGGGGCCAGTCTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGG
ACTTCTTCCTGTACAACGCCTCCAAGGCCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCAT
CGTGCCCTCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCC
GTGGATCGGCCAGTGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAA
GCCCTGATAAGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGC
AGGAGATGACATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAG
TCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGA
AAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCT
CTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCT
TTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCA
AAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCA
CCTCATCAGTCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAA
ACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCT
GCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGG
GTTCTACTGCTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTA
AATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

LFQCFSEVEV*YQ*RQL*ENPQAG*LNPPMDSG
LL*ALRVLQWVSYILMMAIHSNTSTRSNFCTGSF
HSVPVF*SIVLLTRGVIIPASVWWRRSWS*ASG
RSHLL*LPTHLMVKISLWLLRIVPKHPS*AWRS
SHSTLPLTGRVPKKALK*MHSVSFLQDVNVKE*V
GKSV*KHKLPNRKMKFARIPDLSLMEPTELTSV
KES*GTAGFSQPLPA*P*TSTFFSESYPMIKVS
SKTTQGSSTSSSGAMESGWTWL*MTACQRTTINW
FSPSPTTAMSSGVLCWRRLMLSSMVPTKL*KVG
TPQRPWRTSQEGWQSFLRSGMLLVTCTRS*RKP
SREAPSWAAPLMMART*PMEPLLLV*TWGS*LHG
W*GIWITHCSRTQTSTPEAQMKDRPGQSFRFSM
RQEWPAGWSEVTPTLSRGWMRSRSKVRK*SWCG
CGIRGARWSGTVLGVIDGRTGALWTKMRRPVCST
RSLRMESSGCPMRISSTISQSWRSATSRPMLCS
LTSFRPGQCL*TRAAGYGVALPEAAATSQILSG
PTLSTV*SSWRRTMTLMTRR*FAASWWP*CRRTG
GRTGS*GPVSSPLASPSTRFPKRCTGTSSTCRR
TSSCTTPPRPGAKPTSTCGRCPSASACLPASTS
SCPPPTSPTRRGNSSSGSSLKRGTSLRKLKIPSP
WIGQCPSSSFRTEQTATRSWVWTRSQRRAKAKQ
ALISKSSPHSHSLAALIRKVRNSNNSGTFSSR*
QEMTWRSVQMSSRRSLTQS*TNTRT*RHTGSHWS
PAVA*LRSWIQMALESSTCRSSTTSGTRLRPGR
KFSNTMTQTSPAPSTATRCEMQSTTQDSTSTTS
SMTSLPCGTQTNT*TSTLTVSSAASLGWRACSEL
FMHLTRMEMVSSSSTFWSGCSSPCMPEPGWPHP
KPCRITQDFSFTLYFQSHLPQRTQQLHPYRLPG
TSSVMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNK
PLSLCHVEESACLWSEPPRF*SECSCLPCSRLS
AEAPAGGTQHLLVLEPSITFTLSHHGPGTKPAL
GSTAVG*TNSVE*GWLLWAVQLISLAAF*KKLI*
IKACVWLV

Frame 1

YSSVSARWKCDTNKDNCRKIHRLDD*ILLWTPG
CSKH*GFFSG*VIS**WPFIPIPPPEAIFAQEV
FILFQCSDQ*FC*PEGSLSQQVCGGEDLGLRLQE
GAIFCDYPLI*W*RSACGFYVLCQNIHPEPGEA
LTQHCH*LGGFLKKL*NECIVCLSFKM*MSRNE
LANQFKNTNYPTEK*NLRESPIYH*WSQQN*HLS
RRARGLLVSRSHCLPDPEPAPSFPSHTP*SKFH
RKLRRDLPLPVLALWRVGGRGYR*LPANVQQST
GFHQVQPPQ*VLECSAGEGLC*APWFLRSSERWE
HHRGHGGLHRRGGRVF*DQGCS**HVQDHEESH
RERLPHGLLH**WHEHDLWNLSFWSEHGGVDCT
DGKEYG*LTAPGLRPRPQRLR*KTDPDNHSGSV*
DKNGLRAGQRSRLLCHGAG*GPVQR*ESEAGAA
AESVGPGGVERFLE**MEGLELCGQR*EGPSAA
PGH*GWRVLDVL*GFHLPFHKVGDLQPHGRCSAV
*QASDLDSVCERGPLGTGLLCRRLPQLPRYFLD
QPSVPSEAPGGGR*P**LGGDLQLPGGPDAEEP
AEGPEARGQSLHHWLRHLRGSQRDAREQAAPAEG
LLPVQRLQGQEQNLHQHAGGVPALPPASQRVRH
RALHLRAPPGGGIHPPGLL*KEEPL*GS*KYHL
RGSASAHHLRFGQSKQQQGAGCGPGVRGGQRQNK
P**AKAVPTATAWQL*SGK*GTATIPEHFQADS
RR*HGDLCR*AQEGP*HSREQTQGPEDTRVHTG
VLP*HDCAHGYRWLWKAQPAGVPPPLEQD*GLAE
NFQTL*HRPVRHHQQLRDAKCSQRRRIPPQQPA
L*HHYHAVRRQTHEHRL*QFHLLLR*AGGHVQS
FSCI*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQ
SHAGSLRISVSPSISKAIYLKGPSSYTPTGFQA
PHQSCSSSILPPTHP*SVMPSLTL**SNEVGRT
NPCPFAMWRKVPASGPSRLGSEASAPAYLALGCL
QKHLPVALSTSLC*SPPSPSRCPTMGQEPNQHW
VLLLWGKLTQWNRAGYFGLSNS*VWLHFEKS*S
K*RHVYGW

Frame 2

IPVFQRGGSVIPIKTTVGKSTGWMTESSYGLRV
ALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKF
SFCSSVLINSFADQRGHYPSKCVVEKILVLGFR
KEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKL
SLNIATDWEGS*KSSKMNA*CVFPSRCKCQGMS
WQISLKTQTTQQKNEICENPRFIIDGANRTDIC
QGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFI
ENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQL
VFTKSNHRNEFWSALLEKAYAKLHGSYEALKGG
NTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAI
ERGSLMGCSIDDGTNMTYGTSPSGLNMGELIAR
MVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQY
ETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRL
RNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQH
QVTEDGEFWMSYEDFIYHFTKLEICNLTADALQ
SDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWT
NPQYRLKLLEEDDDPDDSEVICSFLVALMQKNR
RKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQK
DFFLYNASKARSKTYINMREVSQRFRLPPSEYVI
VPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS
VDRPVPIIFVSDRANSNKELGVDQESEEGKGKT
SPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIA
GDDMEICADELKKVLNTVVNKHKDLKTHGFTLE
SCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ
KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQL
YDIITMRYADKHMNIDFDSFICCFVRLEGMFRA
FHAFDKDGDGIIKLNVLEWLQLTMYA*TRLASS
KAMQDHSGFQFHPLFPKPFTSKDPAATPLQASRH
LISHAPPPFYPLPILDRSCLA*PFSKAMR*EEQ
TLVPLPCGGKCLPLVRAASVLKRVLLLTLL*AV
CRSTCRWHSAPPCARALHHLHAVPPWARNQTSTG
FYCCGVN*LSGIGLVTLGCPTHKFGCILKKADL
NKGMCMAG

Sequence CAPN3.cAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC
TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT
CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA
CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA
CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA
CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC
TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC
TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT
CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT
GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA
CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC
CATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACTCACTG
CTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCA
GAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTG
GAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGG
ATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGT
CTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCT
GGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGT
CTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGG
CCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCA
GGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGCCCATCATCTTC
GTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATAAGCAAAAGCAGTCCCCAC
AGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGA
TGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATG
GATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGACACAGACC
AGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGC
AGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGAT
GGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGCAGGATCACTCAGGATTTC
AGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGCTCCTCCTCCATT
TTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGA
AAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCACCTGCCGGTGGCACTCAG
CACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGGTAAACTAACT
CAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE
LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL
R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP
LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA
TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH
KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV
WKRPPEICENPRFIIDGANRTDICQGELGDCWF
LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF
QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN
EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF
TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS
IDDGTNMTYGTSPSGLNMGELIARMVRNMDNSL
LQDSDLDPRGSDERPTRTIIPVQYETRMACGLV
RGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEW
NGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFW
MSYEDFIYHFTKLEICNLTADALQSDKLQTWTV
SVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLL
EEDDDPDDSEVICSFLVALMQKNRRKDRKLGAS
LFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASK
ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQ
EGEFILRVFSEKRNLSEEVENTISVDRPVPIIF
VSDRANSNKELGVDQESEEGKGKTSPDKQKQSP
QPQPGSSDQESEEQQQFRNIFKQIAGDDMEICAD
ELKKVLNTVVNKHKDLKTHGFTLESCRSMIALM
DTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTD
QSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYA
DKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGD
GIIKLNVLEWLQLTMYA*TRLASSKAMQDHSGF
QFHPLFPKPFTSKDPAATPLQASRHLISHAPPPF
YPLPILDRSCLA*PFSKAMR*EEQTLVPLPCGG
KCLPLVRAASVLKRVLLLTLL*AVCRSTCRWHS
APPCARALHHLHAVPPWARNQTSTGFYCCGVN*L
SGIGLVTLGCPTHKFGCILKKADLNKGMCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN
LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL
SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH
LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP
LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF
TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS
GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF
SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST
SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM
SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS
QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA
PLMMART*PMEPLLLV*TWGS*LHGW*GIWITHC
SRTQTSTPEAQMKDRPGQSFRFSMRQEWPAGWS
EVTPTLSRGWMRSRSKVRK*SWCGCGIRGARWS
GTVLGVIDGRTGALWTKMRRPVCSTRSLRMESSG
CPMRISSTISQSWRSATSRPMLCSLTSFRPGQC
L*TRAAGYGVALPEAAATSQILSGPTLSTV*SS
WRRTMTLMTRR*FAASWWP*CRRTGGRTGS*GPV
SSPLASPSTRFPKRCTGTSSTCRRTSSCTTPPR
PGAKPTSTCGRCPSASACLPASTSSCPPPTSPT
RRGNSSSGSSLKRGTSLRKLKIPSPWIGQCPSSS
FRTEQTATRSWVWTRSQRRAKAKQALISKSSPH
SHSLAALIRKVRNSNNSGTFSSR*QEMTWRSVQ
MSSRRSLTQS*TNTRT*RHTGSHWSPAVA*LRSW
IQMALESSTCRSSTTSGTRLRPGRKFSNTMTQT
SPAPSTATRCEMQSTTQDSTSTTSSMTSLPCGT
QTNT*TSTLTVSSAASLGWRACSELFMHLTRMEM
VSSSSTFWSGCSSPCMPEPGWPHPKPCRITQDF
SFTLYFQSHLPQRTQQLHPYRLPGTSSVMLLLH
FTPYPSLIGHA*PDPLVKQ*GRKNKPLSLCHVEE
SACLWSEPPRF*SECSCLPCSRLSAEAPAGGTQ
HLLVLEPSITFTLSHHGPGTKPALGSTAVG*TN
SVE*GWLLWAVQLISLAAF*KKLI*IKACVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT
YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS
QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA
TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH
*GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS
QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR
LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS
RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL
PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ
*VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH
RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL
H**WHEHDLWNLSFWSEHGGVDCTDGKEYG*LT
APGLRPRPQRLR*KTDPDNHSGSV*DKNGLRAGQ
RSRLLCHGAG*GPVQR*ESEAGAAAESVGPGGV
ERFLE**MEGLELCGQR*EGPSAAPGH*GWRVL
DVL*GFHLPFHKVGDLQPHGRCSAV*QASDLDSV
CERGPLGTGLLCRRLPQLPRYFLDQPSVPSEAP
GGGR*P**LGGDLQLPGGPDAEEPAEGPEARGQ
SLHHWLRHLRGSQRDAREQAAPAEGLLPVQRLQG
QEQNLHQHAGGVPALPPASQRVRHRALHLRAPP
GGGIHPPGLL*KEEPL*GS*KYHLRGSASAHHL
RFGQSKQQQGAGCGPGVRGGQRQNKP**AKAVPT
ATAWQL*SGK*GTATIPEHFQADSRR*HGDLCR
*AQEGP*HSREQTQGPEDTRVHTGVLP*HDCAH
GYRWLWKAQPAGVPPPLEQD*GLAENFQTL*HRP
VRHHQQLRDAKCSQRRRIPPQQPAL*HHYHAVR
RQTHEHRL*QFHLLLR*AGGHVQSFSCI*QGWR
WYHQAQRSGVAAAHHVCLNQAGLIQSHAGSLRIS
VSPSISKAIYLKGPSSYTPTGFQAPHQSCSSSI
LPPTHP*SVMPSLTL**SNEVGRTNPCPFAMWR
KVPASGPSRLGSEASAPAYLALGCLQKHLPVALS
TSLC*SPPSPSRCPTMGQEPNQHWVLLLWGKLT
QWNRAGYFGLSNS*VWLHFEKS*SK*RHVYGW

Sequence CAPN3.bAug05

CACTCTCTTTCTCTCTCCCTCTGGCATGCATGCTGCTGGTAGGAGACCCCCAAGTCAACATTGCTTCAGAAATCCTTTAGCACTCATTTCTCAGGAGAAC
TTATGGCTTCAGAATCACAGCTCGGTTTTTAAGATGGACATAACCTGTACGACCTTCTGATGGGCTTTCAACTTTGAACTGGATGTGGACACTTTTCTCT
CAGATGACAGAATTACTCCAACTTCCCCTTTGCAGTTGCTTCCTTTCCTTGAAGGTAGCTGTATCTTATTTTCTTTAAAAAGCTTTTTCTTCCAAAGCCA
CTTGCCATGCCGACCGTCATTAGCGCATCTGTGGCTCCAAGGACAGCGGCTGAGCCCCGGTCCCCAGGGCCAGTTCCTCACCCGGCCCAGAGCAAGGCCA
CTGAGGCTGGGGGTGGAAACCCAAGTGGCATCTATTCAGCCATCATCAGCCGCAATTTTCCTATTATCGGAGTGAAAGAGAAGACATTCGAGCAACTTCA
CAAGAAATGTCTAGAAAAGAAAGTTCTTTATGTGGACCCTGAGTTCCCACCGGATGAGACCTCTCTCTTTTATAGCCAGAAGTTCCCCATCCAGTTCGTC
TGGAAGAGACCTCCGGAAATTTGCGAGAATCCCCGATTTATCATTGATGGAGCCAACAGAACTGACATCTGTCAAGGAGAGCTAGGGGACTGCTGGTTTC
TCGCAGCCATTGCCTGCCTGACCCTGAACCAGCACCTTCTTTTCCGAGTCATACCCCATGATCAAAGTTTCATCGAAAACTACGCAGGGATCTTCCACTT
CCAGTTCTGGCGCTATGGAGAGTGGGTGGACGTGGTTATAGATGACTGCCTGCCAACGTACAACAATCAACTGGTTTTCACCAAGTCCAACCACCGCAAT
GAGTTCTGGAGTGCTCTGCTGGAGAAGGCTTATGCTAAGCTCCATGGTTCCTACGAAGCTCTGAAAGGTGGGAACACCACAGAGGCCATGGAGGACTTCA
CAGGAGGGGTGGCAGAGTTTTTTGAGATCAGGGATGCTCCTAGTGACATGTACAAGATCATGAAGAAAGCCATCGAGAGAGGCTCCCTCATGGGCTGCTC
CATTGATGATGGCACGAACATGACCTATGGAACCTCTCCTTCTGGTCTGAACATGGGGGAGTTGATTGCACGGATGGTAAGGAATATGGATAACTCACTG
CTCCAGGACTCAGACCTCGACCCCAGAGGCTCAGATGAAAGACCGACCCGGACAATCATTCCGGTTCAGTATGAGACAAGAATGGCCTGCGGGCTGGTCA
GAGGTCACGCCTACTCTGTCACGGGGCTGGATGAGGTCCCGTTCAAAGGTGAGAAAGTGAAGCTGGTGCGGCTGCGGAATCCGTGGGGCCAGGTGGAGTG
GAACGGTTCTTGGAGTGATAGATGGAAGGACTGGAGCTTTGTGGACAAAGATGAGAAGGCCCGTCTGCAGCACCAGGTCACTGAGGATGGAGAGTTCTGG
ATGTCCTATGAGGATTTCATCTACCATTTCACAAAGTTGGAGATCTGCAACCTCACGGCCGATGCTCTGCAGTCTGACAAGCTTCAGACCTGGACAGTGT
CTGTGAACGAGGGCCGCTGGGTACGGGGTTGCTCTGCCGGAGGCTGCCGCAACTTCCCAGATACTTTCTGGACCAACCCTCAGTACCGTCTGAAGCTCCT
GGAGGAGGACGATGACCCTGATGACTCGGAGGTGATTTGCAGCTTCCTGGTGGCCCTGATGCAGAAGAACCGGCGGAAGGACCGGAAGCTAGGGGCCAGT
CTCTTCACCATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGGGAACAAGCAGCACCTGCAGAAGGACTTCTTCCTGTACAACGCCTCCAAGG
CCAGGAGCAAAACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCTCCCAGCGAGTACGTCATCGTGCCCTCCACCTACGAGCCCCACCA
GGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAACCTCTCTGAGGAAGTTGAAAATACCATCTCCGTGGATCGGCCAGTGAAAAAGAAAAAA
ACCAAGCCCATCATCTTCGTTTCGGACAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGTCAGAGGAGGGCAAAGGCAAAACAAGCCCTGATA
AGCAAAAGCAGTCCCCACAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGA
CATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGT
AGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCA
AACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACAT
CATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCA
TTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCATCCAAAGCCATGC
AGGATCACTCAGGATTTCAGTTTCACCCTCTATTTCCAAAGCCATTTACCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAG
TCATGCTCCTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTAAAGCAATGAGGTAGGAAGAACAAACCCTTGTC
CCTTTGCCATGTGGAGGAAAGTGCCTGCCTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTGTCTGCAGAAGCA
CCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACCTTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTG
CTGTGGGGTAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCTGCATTTTGAAAAAAGCTGATCTAAATAAAGGC
ATGTGTATGGCTGGTC
  • CAPN3.u.f.38 (forward primer)
  • CAPN3.u.r.40 (reverse primer)
  • Single exon skipping

Frame 0

HSLSLSLWHACCW*ETPKSTLLQKSFSTHFSGE
LMASESQLGF*DGHNLYDLLMGFQL*TGCGHFSL
R*QNYSNFPFAVASFP*R*LYLIFFKKLFLPKP
LAMPTVISASVAPRTAAEPRSPGPVPHPAQSKA
TEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLH
KKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFV
WKRPPEICENPRFIIDGANRTDICQGELGDCWF
LAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHF
QFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRN
EFWSALLEKAYAKLHGSYEALKGGNTTEAMEDF
TGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCS
IDDGTNMTYGTSPSGLNMGELIARMVRNMDNSL
LQDSDLDPRGSDERPTRTIIPVQYETRMACGLV
RGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEW
NGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFW
MSYEDFIYHFTKLEICNLTADALQSDKLQTWTV
SVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLL
EEDDDPDDSEVICSFLVALMQKNRRKDRKLGAS
LFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASK
ARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQ
EGEFILRVFSEKRNLSEEVENTISVDRPVKKKK
TKPIIFVSDRANSNKELGVDQESEEGKGKTSPD
KQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDD
MEICADELKKVLNTVVNKHKDLKTHGFTLESCR
SMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIF
KHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDI
ITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA
FDKDGDGIIKLNVLEWLQLTMYA*TRLASSKAM
QDHSGFQFHPLFPKPFTSKDPAATPLQASRHLIS
HAPPPFYPLPILDRSCLA*PFSKAMR*EEQTLV
PLPCGGKCLPLVRAASVLKRVLLLTLL*AVCRS
TCRWHSAPPCARALHHLHAVPPWARNQTSTGFYC
CGVN*LSGIGLVTLGCPTHKFGCILKKADLNKG
MCMAG

Frame 1

TLFLSPSGMHAAGRRPPSQHCFRNPLALISQEN
LWLQNHSSVFKMDITCTTF*WAFNFELDVDTFL
SDDRITPTSPLQLLPFLEGSCILFSLKSFFFQSH
LPCRPSLAHLWLQGQRLSPGPQGQFLTRPRARP
LRLGVETQVASIQPSSAAIFLLSE*KRRHSSNF
TRNV*KRKFFMWTLSSHRMRPLSFIARSSPSSSS
GRDLRKFARIPDLSLMEPTELTSVKES*GTAGF
SQPLPA*P*TSTFFSESYPMIKVSSKTTQGSST
SSSGAMESGWTWL*MTACQRTTINWFSPSPTTAM
SSGVLCWRRLMLSSMVPTKL*KVGTPQRPWRTS
QEGWQSFLRSGMLLVTCTRS*RKPSREAPSWAA
PLMMART*PMEPLLLV*TWGS*LHGW*GIWITHC
SRTQTSTPEAQMKDRPGQSFRFSMRQEWPAGWS
EVTPTLSRGWMRSRSKVRK*SWCGCGIRGARWS
GTVLGVIDGRTGALWTKMRRPVCSTRSLRMESSG
CPMRISSTISQSWRSATSRPMLCSLTSFRPGQC
L*TRAAGYGVALPEAAATSQILSGPTLSTV*SS
WRRTMTLMTRR*FAASWWP*CRRTGGRTGS*GPV
SSPLASPSTRFPKRCTGTSSTCRRTSSCTTPPR
PGAKPTSTCGRCPSASACLPASTSSCPPPTSPT
RRGNSSSGSSLKRGTSLRKLKIPSPWIGQ*KRKK
PSPSSSFRTEQTATRSWVWTRSQRRAKAKQALI
SKSSPHSHSLAALIRKVRNSNNSGTFSSR*QEM
TWRSVQMSSRRSLTQS*TNTRT*RHTGSHWSPAV
A*LRSWIQMALESSTCRSSTTSGTRLRPGRKFS
NTMTQTSPAPSTATRCEMQSTTQDSTSTTSSMT
SLPCGTQTNT*TSTLTVSSAASLGWRACSELFMH
LTRMEMVSSSSTFWSGCSSPCMPEPGWPHPKPC
RITQDFSFTLYFQSHLPQRTQQLHPYRLPGTSS
VMLLLHFTPYPSLIGHA*PDPLVKQ*GRKNKPLS
LCHVEESACLWSEPPRF*SECSCLPCSRLSAEA
PAGGTQHLLVLEPSITFTLSHHGPGTKPALGST
AVG*TNSVE*GWLLWAVQLISLAAF*KKLI*IKA
CVWLV

Frame 2

LSFSLPLACMLLVGDPQVNIASEIL*HSFLRRT
YGFRITARFLRWT*PVRPSDGLSTLNWMWTLFS
QMTELLQLPLCSCFLSLKVAVSYFL*KAFSSKA
TCHADRH*RICGSKDSG*APVPRASSSPGPEQGH
*GWGWKPKWHLFSHHQPQFSYYRSEREDIRATS
QEMSRKESSLCGP*VPTG*DLSLL*PEVPHPVR
LEETSGNLRESPIYH*WSQQN*HLSRRARGLLVS
RSHCLPDPEPAPSFPSHTP*SKFHRKLRRDLPL
PVLALWRVGGRGYR*LPANVQQSTGFHQVQPPQ
*VLECSAGEGLC*APWFLRSSERWEHHRGHGGLH
RRGGRVF*DQGCS**HVQDHEESHRERLPHGLL
H**WHEHDLWNLSFWSEHGGVDCTDGKEYG*LT
APGLRPRPQRLR*KTDPDNHSGSV*DKNGLRAGQ
RSRLLCHGAG*GPVQR*ESEAGAAAESVGPGGV
ERFLE**MEGLELCGQR*EGPSAAPGH*GWRVL
DVL*GFHLPFHKVGDLQPHGRCSAV*QASDLDSV
CERGPLGTGLLCRRLPQLPRYFLDQPSVPSEAP
GGGR*P**LGGDLQLPGGPDAEEPAEGPEARGQ
SLHHWLRHLRGSQRDAREQAAPAEGLLPVQRLQG
QEQNLHQHAGGVPALPPASQRVRHRALHLRAPP
GGGIHPPGLL*KEEPL*GS*KYHLRGSASEKEK
NQAHHLRFGQSKQQQGAGCGPGVRGGQRQNKP**
AKAVPTATAWQL*SGK*GTATIPEHFQADSRR*
HGDLCR*AQEGP*HSREQTQGPEDTRVHTGVLP
*HDCAHGYRWLWKAQPAGVPPPLEQD*GLAENFQ
TL*HRPVRHHQQLRDAKCSQRRRIPPQQPAL*H
HYHAVRRQTHEHRL*QFHLLLR*AGGHVQSFSC
I*QGWRWYHQAQRSGVAAAHHVCLNQAGLIQSHA
GSLRISVSPSISKAIYLKGPSSYTPTGFQAPHQ
SCSSSILPPTHP*SVMPSLTL**SNEVGRTNPC
PFAMWRKVPASGPSRLGSEASAPAYLALGCLQKH
LPVALSTSLC*SPPSPSRCPTMGQEPNQHWVLL
LWGKLTQWNRAGYFGLSNS*VWLHFEKS*SK*R
HVYGW

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