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Sequences

Sequence BCL2L1.hAug05

GGCTGCGCTGGGGCGGACCGCCGGGGGCGAGGCCCCTCCTCTCAGGAAGGTCTAGGGGGCGCCCCCGGGGGAGGGCGGTTGCCTAGCAACGGGGCGGTGG CTCGGCGGGTACCCGGCCTAGTGAGCTCGAGAAGCCGCTAGGCCGCAGCCGGCCCACCCTGGGCTCCGGAGAGGTGCAGCCCCCGGCCCCGGAGCCCTCC CGGGGGCGCCAGAAAGCCACTTTGGGCCTGTCCCCAGGAAAACGTGGTCTCAGCTGTGGGGCCGTCGAGCAGGCCTCGACCCCTCTTCCTGACCTGAGGA GAGGCCCAGCGCCTCACCCTCACCCAGTCTTTGTGCAGGGCGCCGGCGGCCATTGTGTCCGGGCGGGGAATGGAGGACCCGGCAATCCCCAACTGCCACG TTCTGGGCCTTTGGGGAATTCAGAGCAAACCAGCGGCATTTGTTGGGGGTCTCCGGCCTTCAACATCACAGACAGGCCTGGGGGTGGCCTTCCCAAAGTC AGATTGCAGATCTGAGGCAGTTTCCCCCTCCCTGCGTCCCTCACTGAAACCTTGAACCCCATTGAGAAGTCCCTTTAGGGTTTCGGACGCCTCCACCTCA CCCTGGGCTGGTGCTTAAATAGAAAAAAGAAAAACAAAAACCAACTAAATCCATACCAGCCACCTCCGGGAGAGTACTCCTGGCTCCCAGTAGGAGGCGG AGAGCCAAGGGGCGTGCAAGAGAGAGGGGGCTGGGCTCCCGGGTGGCAGGAGGCCGCGGCTGCGGAGCGGCCGCCCTCGATCCGGGCGATGGAGGAGGAA GCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTT TTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGCACCTGCC TGCCTTTGCCTAAGGCGGATTTGAATCTCTTTCTCTCCCTTCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCA GTGAGTGAGCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTA CAAGCTTTCCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCC AGTGCCATCAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCC CCATGGCAGCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCAC CCCAGGGACAGCATATCAGAGCTTTGAACAGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAAC CGCTGGTTCCTGACGGGCATGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCA CCCCCTTCTCTGCTCCACCACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGC CCAGATCTGGTCCCTTGCAGCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCA CAAAATCTGTCCAAAGGAGGCTGGCAGGTATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCT GAAAATGTTTTTCTGCCAGGGAGCTTGAAAGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTG ATCATTTTCCCCCCACTCTCCCCACACTAACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCA GGCTGCTTGGGATAAAGATGCAAGGACCAGGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGG CTAGAGGCCAGCCCCACCCAGGAGGGAGGGGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCC CTTCCCCTCCCTGGCTCCCATGACCATACTGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAA GAGCAGCCTGTGGCATCTTTGCCTTGGGCTGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGC CCCAGGGTCTTCCCTACCTCAGGCAGGAAGGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGG CAGATCTGTGCCCCATGCCTGTCCAGCCTGGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCC ACCCTTGGCCTGTCTCATCCCCAGGGGTCCCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTAT TTTTGTTAAGCGTGTCTGTATTTATGTGTGAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTC CCTCCCCTGCCCTGGTCCAGGGAAGCTGGCCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTT GAAATAGTCTGTGTGAAGGTGAAAGTGCAGTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCL2L1.u.f.5 (forward primer)
BCL2L1.u.r.5 (reverse primer)
Alternative donor

Frame 0

GCAGADRRGRGPSSQEGLGGAPGGGRLPSNGAV ARRVPGLVSSRSR*AAAGPPWAPERCSPRPRSPP GGARKPLWACPQENVVSAVGPSSRPRPLFLT*G EAQRLTLTQSLCRAPAAIVSGRGMEDPAIPNCH VLGLWGIQSKPAAFVGGLRPSTSQTGLGVAFPKS DCRSEAVSPSLRPSLKP*TPLRSPFRVSDASTS PWAGA*IEKRKTKTN*IHTSHLRESTPGSQ*EA ESQGACKREGAGLPGGRRPRLRSGRPRSGRWRRK QARGLVPELRNSCVAFWAPSLPGRMIPPAGAGF FASHREAG*VTGIPAATSSPDL*YKRSSGGCTC LPLPKADLNLFLSLQNLILALDLRRESLTRDETQ *VSRCFGQWTG*AHPYYKNVSEQPGAGG*LSLL QAFPERIQLESV**CGREQD*GPRRD*IGDGDP QCHQWQPILAPGRQPRGEWSHWPQQQFGCPGGDP HGSSKASAEGGRRRV*TAVPAGIQ*PDIPAPHH PRDSISEL*TGYFCGTLWEQCSSREPKGPGTLQ PLVPDGHDCGRRGSAGLTLQSEMTRH*PSTLPSH PLLCSTTSSVQPPLPPGEPLHAAHAHLPITGLG PDLVPCS*FSRIYHTSVRPPHLSSLGLRIHKIS TKSVQRRLAGMEGFVAGGRRALPDWCNPYPLASL KMFFCQGA*KFSEPLPQKGD*IAFVLMFVASEL IIFPPLSPH*PGFPFLPSLPPKSHLGATFD*GF RLLGIKMQGPGLPPHLWTG*SPHSQSKCPPEASG *RPAPPRREGAIATGSTPCQS*GGPCSSAPP*S LPLPGSHDHTEGPTGPKTDAPELFMASAASLPT RAACGIFALGCSSWWVQGTQP*GERELSGTAMGA PGSSLPQAGRAGRRACCMGWGRAD*KGQSCLAR QICAPCLSSLGSQAAKARVAWPGALQASLSLLL HPWPVSSPGVPATPGSLLYIFETSFYSL*R*YTI FVKRVCIYV*GAAGLQCACTWRAGARRLDGLML PPLPWSREAGRGSWLLRGICPSPNPHPTLVPAL *NSLCEGESAVQ**TVFTQ*TKS

Frame 1

AALGRTAGGEAPPLRKV*GAPPGEGGCLATGRW LGGYPA**AREAARPQPAHPGLRRGAAPGPGAL PGAPESHFGPVPRKTWSQLWGRRAGLDPSS*PEE RPSASPSPSLCAGRRRPLCPGGEWRTRQSPTAT FWAFGEFRANQRHLLGVSGLQHHRQAWGWPSQS QIADLRQFPPPCVPH*NLEPH*EVPLGFRTPPPH PGLVLK*KKEKQKPTKSIPATSGRVLLAPSRRR RAKGRARERGLGSRVAGGRGCGAAALDPGDGGG SKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVF LPATARPAELPASPQPPPLPTCDTKDLPGAAPA CLCLRRI*ISFSPFRILSWLWILEENH*PETRL SE*AGVLDNGLVEPIPIIKMSQSNRELVVDFLSY KLSQKGYSWSQFSDVEENRTEAPEGTESEMETP SAINGNPSWHLADSPAVNGATGHSSSLDAREVI PMAAVKQALREAGDEFELRYRRAFSDLTSQLHIT PGTAYQSFEQDTFVELYGNNAAAESRKGQERFN RWFLTGMTVAGVVLLGSLFSRK*PDTDHPLYPP TPFSAPPHPPSSRHCHQENHYMQPMPTCPSQGWA QIWSLAASFLEFITLL*DPHTSVPLASEFTKFP QNLSKGGWQVWKGLWLGAGGPYLIGATLTP*PP *KCFSARELESFQNLFPRKETRLPLF*CLWPQN* SFSPHSPHTNLGSLSFHPYPLRAI*GPLLTRDS GCLG*RCKDQDSLLTSGLARVLTPSPNVLQKPL ARGQPHPGGRGL*LQEAPHAKARVALAVQHHPSP FPSLAPMTILRDQLGPRQMPQSCLWPQLPHFLQ EQPVASLPWAAPHGGFRGLSPEVKGSYQEQLWE PQGLPYLRQEGQEGEPAAWGGVGLTRRASPAWPG RSVPHACPAWAARLPRPEWPGQELFRPPSLFCS TLGLSHPQGSQPPRALCCTYLRLVFIPCEDDIL FLLSVSVFMCEELLACSARARGELVPGDWTA*CS LPCPGPGKLAEGPGS*GASAPPPTPTPHLFQLF EIVCVKVKVQFSNKLCLLSEQRA

Frame 2

LRWGGPPGARPLLSGRSRGRPRGRAVA*QRGGG SAGTRPSELEKPLGRSRPTLGSGEVQPPAPEPS RGRQKATLGLSPGKRGLSCGAVEQASTPLPDLR RGPAPHPHPVFVQGAGGHCVRAGNGGPGNPQLPR SGPLGNSEQTSGICWGSPAFNITDRPGGGLPKV RLQI*GSFPLPASLTETLNPIEKSL*GFGRLHL TLGWCLNRKKKNKNQLNPYQPPPGEYSWLPVGGG EPRGVQERGGWAPGWQEAAAAERPPSIRAMEEE ASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWF FCQPPRGRLSYRHPRSHLLSRPVIQKIFRGLHLP AFA*GGFESLSLPSESYLGFGS*KRITNQRRDS VSEQVFWTMDWLSPSLL*KCLRATGSWWLTFSP TSFPRKDTAGVSLVMWKRTGLRPQKGLNRRWRPP VPSMATHPGTWQTAPR*MEPLATAAVWMPGR*S PWQQ*SKR*GRQATSLNCGTGGHSVT*HPSSTS PQGQHIRALNRILLWNSMGTMQQPRAERARNAST AGS*RA*LWPAWFCWAHSSVGNDQTLTIHSTLP PPSLLHHILRPAAIATRRTTTCSPCPPAHHRVG PRSGPLQLVF*NLSHFCETPTPQFPWPQNSQNFH KICPKEAGRYGRVCGWGQEGPT*LVQPLPLSLP ENVFLPGSLKVFRTSSPERRLDCLCFDVCGLRI DHFPPTLPTLTWVPFPSIPTP*EPFRGHF*LGIQ AAWDKDARTRTPSSPLDWLESSLPVQMSSRSLW LEASPTQEGGGYSYRKHPMPKLGWPLQFSTTLV PSPPWLP*PY*GTNWAQDRCPRAVYGLSCLTSYK SSLWHLCLGLLLMVGSGDSALR*KGAIRNSYGS PRVFPTSGRKGRKESLLHGVG*G*LEGPVLPGQ ADLCPMPVQPGQPGCQGQSGLARSSSGLPLSSAP PLACLIPRGPSHPGLSAVHI*D*FLFLVKMIYY FC*ACLYLCVRSCWLAVRVHVESWCPEIGRPDA PSPALVQGSWPRVLAPEGHLPLPQPPPHTCSSSL K*SV*R*KCSSVINCVYSVNKE

Sequence BCL2L1.dAug05

GGCTGCGCTGGGGCGGACCGCCGGGGGCGAGGCCCCTCCTCTCAGGAAGGTCTAGGGGGCGCCCCCGGGGGAGGGCGGTTGCCTAGCAACGGGGCGGTGG CTCGGCGGGTACCCGGCCTAGTGAGCTCGAGAAGCCGCTAGGCCGCAGCCGGCCCACCCTGGGCTCCGGAGAGGTGCAGCCCCCGGCCCCGGAGCCCTCC CGGGGGCGCCAGAAAGCCACTTTGGGCCTGTCCCCAGGAAAACGTGGTCTCAGCTGTGGGGCCGTCGAGCAGGCCTCGACCCCTCTTCCTGACCTGAGGA GAGGCCCAGCGCCTCACCCTCACCCAGTCTTTGTGCAGGGCGCCGGCGGCCATTGTGTCCGGGCGGGGAATGGAGGACCCGGCAATCCCCAACTGCCACG TTCTGGGCCTTTGGGGAATTCAGAGCAAACCAGCGGCATTTGTTGGGGGTCTCCGGCCTTCAACATCACAGACAGGCCTGGGGGTGGCCTTCCCAAAGTC AGATTGCAGATCTGAGGCAGTTTCCCCCTCCCTGCGTCCCTCACTGAAACCTTGAACCCCATTGAGAAGTCCCTTTAGGGTTTCGGACGCCTCCACCTCA CCCTGGGCTGGTGCTTAAATAGAAAAAAGAAAAACAAAAACCAACTAAATCCATACCAGCCACCTCCGGGAGAGTACTCCTGGCTCCCAGTAGGAGGCGG AGAGCCAAGGGGCGTGCAAGAGAGAGGGGGCTGGGCTCCCGGGTGGCAGGAGGCCGCGGCTGCGGAGCGGCCGCCCTCGATCCGGGCGATGGAGGAGGAA GCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTT TTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGCACCTGCC TGCCTTTGCCTAAGGCGGATTTGAATCTCTTTCTCTCCCTTCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCA GTGAGTGAGCAGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTA CAAGCTTTCCCAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCC AGTGCCATCAATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCC CCATGGCAGCAGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCAC CCCAGGGACAGCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGG GCACTGTGCGTGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGA TCCAGGAGAACGGCGGCTGGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCT GACGGGCATGACTGTGGCCGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCT GCTCCACCACATCCTCCGTCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGT CCCTTGCAGCTAGTTTTCTAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCACAAAATCTGTC CAAAGGAGGCTGGCAGGTATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCTGAAAATGTTTT TCTGCCAGGGAGCTTGAAAGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTGATCATTTTCCC CCCACTCTCCCCACACTAACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCAGGCTGCTTGGG ATAAAGATGCAAGGACCAGGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGGCTAGAGGCCAG CCCCACCCAGGAGGGAGGGGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCCCTTCCCCTCCC TGGCTCCCATGACCATACTGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAAGAGCAGCCTGT GGCATCTTTGCCTTGGGCTGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGCCCCAGGGTCTT CCCTACCTCAGGCAGGAAGGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGGCAGATCTGTGC CCCATGCCTGTCCAGCCTGGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCCACCCTTGGCCT GTCTCATCCCCAGGGGTCCCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTATTTTTGTTAAGC GTGTCTGTATTTATGTGTGAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTCCCTCCCCTGCC CTGGTCCAGGGAAGCTGGCCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTTGAAATAGTCTG TGTGAAGGTGAAAGTGCAGTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC

BCL2L1.u.f.5 (forward primer)
BCL2L1.u.r.5 (reverse primer)
Alternative donor

Frame 0

GCAGADRRGRGPSSQEGLGGAPGGGRLPSNGAV ARRVPGLVSSRSR*AAAGPPWAPERCSPRPRSPP GGARKPLWACPQENVVSAVGPSSRPRPLFLT*G EAQRLTLTQSLCRAPAAIVSGRGMEDPAIPNCH VLGLWGIQSKPAAFVGGLRPSTSQTGLGVAFPKS DCRSEAVSPSLRPSLKP*TPLRSPFRVSDASTS PWAGA*IEKRKTKTN*IHTSHLRESTPGSQ*EA ESQGACKREGAGLPGGRRPRLRSGRPRSGRWRRK QARGLVPELRNSCVAFWAPSLPGRMIPPAGAGF FASHREAG*VTGIPAATSSPDL*YKRSSGGCTC LPLPKADLNLFLSLQNLILALDLRRESLTRDETQ *VSRCFGQWTG*AHPYYKNVSEQPGAGG*LSLL QAFPERIQLESV**CGREQD*GPRRD*IGDGDP QCHQWQPILAPGRQPRGEWSHWPQQQFGCPGGDP HGSSKASAEGGRRRV*TAVPAGIQ*PDIPAPHH PRDSISEL*TGSE*TLPGWGKLGSHCGLFLLRR GTVRGKRRQGDAGIGESDRSLDGHLPE*PPRALD PGERRLGYFCGTLWEQCSSREPKGPGTLQPLVP DGHDCGRRGSAGLTLQSEMTRH*PSTLPSHPLL CSTTSSVQPPLPPGEPLHAAHAHLPITGLGPDLV PCS*FSRIYHTSVRPPHLSSLGLRIHKISTKSV QRRLAGMEGFVAGGRRALPDWCNPYPLASLKMF FCQGA*KFSEPLPQKGD*IAFVLMFVASELIIFP PLSPH*PGFPFLPSLPPKSHLGATFD*GFRLLG IKMQGPGLPPHLWTG*SPHSQSKCPPEASG*RP APPRREGAIATGSTPCQS*GGPCSSAPP*SLPLP GSHDHTEGPTGPKTDAPELFMASAASLPTRAAC GIFALGCSSWWVQGTQP*GERELSGTAMGAPGS SLPQAGRAGRRACCMGWGRAD*KGQSCLARQICA PCLSSLGSQAAKARVAWPGALQASLSLLLHPWP VSSPGVPATPGSLLYIFETSFYSL*R*YTIFVK RVCIYV*GAAGLQCACTWRAGARRLDGLMLPPLP WSREAGRGSWLLRGICPSPNPHPTLVPAL*NSL CEGESAVQ**TVFTQ*TKS

Frame 1

AALGRTAGGEAPPLRKV*GAPPGEGGCLATGRW LGGYPA**AREAARPQPAHPGLRRGAAPGPGAL PGAPESHFGPVPRKTWSQLWGRRAGLDPSS*PEE RPSASPSPSLCAGRRRPLCPGGEWRTRQSPTAT FWAFGEFRANQRHLLGVSGLQHHRQAWGWPSQS QIADLRQFPPPCVPH*NLEPH*EVPLGFRTPPPH PGLVLK*KKEKQKPTKSIPATSGRVLLAPSRRR RAKGRARERGLGSRVAGGRGCGAAALDPGDGGG SKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVF LPATARPAELPASPQPPPLPTCDTKDLPGAAPA CLCLRRI*ISFSPFRILSWLWILEENH*PETRL SE*AGVLDNGLVEPIPIIKMSQSNRELVVDFLSY KLSQKGYSWSQFSDVEENRTEAPEGTESEMETP SAINGNPSWHLADSPAVNGATGHSSSLDAREVI PMAAVKQALREAGDEFELRYRRAFSDLTSQLHIT PGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGG ALCVESVDKEMQVLVSRIAAWMATYLNDHLEPW IQENGGWDTFVELYGNNAAAESRKGQERFNRWFL TGMTVAGVVLLGSLFSRK*PDTDHPLYPPTPFS APPHPPSSRHCHQENHYMQPMPTCPSQGWAQIW SLAASFLEFITLL*DPHTSVPLASEFTKFPQNLS KGGWQVWKGLWLGAGGPYLIGATLTP*PP*KCF SARELESFQNLFPRKETRLPLF*CLWPQN*SFS PHSPHTNLGSLSFHPYPLRAI*GPLLTRDSGCLG *RCKDQDSLLTSGLARVLTPSPNVLQKPLARGQ PHPGGRGL*LQEAPHAKARVALAVQHHPSPFPS LAPMTILRDQLGPRQMPQSCLWPQLPHFLQEQPV ASLPWAAPHGGFRGLSPEVKGSYQEQLWEPQGL PYLRQEGQEGEPAAWGGVGLTRRASPAWPGRSV PHACPAWAARLPRPEWPGQELFRPPSLFCSTLGL SHPQGSQPPRALCCTYLRLVFIPCEDDILFLLS VSVFMCEELLACSARARGELVPGDWTA*CSLPC PGPGKLAEGPGS*GASAPPPTPTPHLFQLFEIVC VKVKVQFSNKLCLLSEQRA

Frame 2

LRWGGPPGARPLLSGRSRGRPRGRAVA*QRGGG SAGTRPSELEKPLGRSRPTLGSGEVQPPAPEPS RGRQKATLGLSPGKRGLSCGAVEQASTPLPDLR RGPAPHPHPVFVQGAGGHCVRAGNGGPGNPQLPR SGPLGNSEQTSGICWGSPAFNITDRPGGGLPKV RLQI*GSFPLPASLTETLNPIEKSL*GFGRLHL TLGWCLNRKKKNKNQLNPYQPPPGEYSWLPVGGG EPRGVQERGGWAPGWQEAAAAERPPSIRAMEEE ASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWF FCQPPRGRLSYRHPRSHLLSRPVIQKIFRGLHLP AFA*GGFESLSLPSESYLGFGS*KRITNQRRDS VSEQVFWTMDWLSPSLL*KCLRATGSWWLTFSP TSFPRKDTAGVSLVMWKRTGLRPQKGLNRRWRPP VPSMATHPGTWQTAPR*MEPLATAAVWMPGR*S PWQQ*SKR*GRQATSLNCGTGGHSVT*HPSSTS PQGQHIRALNR**MNSSGMG*TGVALWPFSPSAG HCAWKA*TRRCRYW*VGSQLGWPLT*MTT*SLG SRRTAAGILLWNSMGTMQQPRAERARNASTAGS *RA*LWPAWFCWAHSSVGNDQTLTIHSTLPPPSL LHHILRPAAIATRRTTTCSPCPPAHHRVGPRSG PLQLVF*NLSHFCETPTPQFPWPQNSQNFHKIC PKEAGRYGRVCGWGQEGPT*LVQPLPLSLPENVF LPGSLKVFRTSSPERRLDCLCFDVCGLRIDHFP PTLPTLTWVPFPSIPTP*EPFRGHF*LGIQAAW DKDARTRTPSSPLDWLESSLPVQMSSRSLWLEAS PTQEGGGYSYRKHPMPKLGWPLQFSTTLVPSPP WLP*PY*GTNWAQDRCPRAVYGLSCLTSYKSSL WHLCLGLLLMVGSGDSALR*KGAIRNSYGSPRVF PTSGRKGRKESLLHGVG*G*LEGPVLPGQADLC PMPVQPGQPGCQGQSGLARSSSGLPLSSAPPLA CLIPRGPSHPGLSAVHI*D*FLFLVKMIYYFC*A CLYLCVRSCWLAVRVHVESWCPEIGRPDAPSPA LVQGSWPRVLAPEGHLPLPQPPPHTCSSSLK*S V*R*KCSSVINCVYSVNKE

Sequence BCL2L1.jAug05

GGAGGAAGCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGC TGGTTTTTTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGC ACCTGCCTGCCTTTGCCTAAGGCGGATTTGAATGTAGGTGGTGCGGGGGAGCGGGAGTGGGGCGGGGGGGACTGCCCAGGGAGTGACTTTCCGAGGAAGG CATTTCGGAGAAGACGGGGGTAGAAAAGGCTGGTGGGAGATTCAGAGTCCACTGGTGCTTTCGATTTGACTTAAGTGAAGTATCTTGGAACCTAGACCCA GACCTTCCTCTTTCTCTCCCTTCAGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGAGCAGGTGTTTT GGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTTCCCAGAAAGGA TACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCATCAATGGCAACC CATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCAGCAGTAAAGCA AGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGACAGCATATCAG AGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTGCGTGGAAAGCG TAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAGAACGGCGGCTG GGTAAGAACC

BCL2L1.u.f.5 (forward primer)
BCL2L1.u.r.5 (reverse primer)
Alternative donor

Frame 0

GGSKRGGWFLSFAIPVSPSGLPACRVA*SLRPE LVFLPATARPAELPASPQPPPLPTCDTKDLPGAA PACLCLRRI*M*VVRGSGSGAGGTAQGVTFRGR HFGEDGGRKGWWEIQSPLVLSI*LK*SILEPRP RPSSFSPFRILSWLWILEENH*PETRLSE*AGVL DNGLVEPIPIIKMSQSNRELVVDFLSYKLSQKG YSWSQFSDVEENRTEAPEGTESEMETPSAINGN PSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQ ALREAGDEFELRYRRAFSDLTSQLHITPGTAYQ SFEQVVNELFRDGVNWGRIVAFFSFGGALCVES VDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGW VRT

Frame 1

EEASEGAGS*ASQFLCRLLGSQPAGSHDPSGRS WFFCQPPRGRLSYRHPRSHLLSRPVIQKIFRGL HLPAFA*GGFECRWCGGAGVGRGGLPRE*LSEEG ISEKTGVEKAGGRFRVHWCFRFDLSEVSWNLDP DLPLSLPSESYLGFGS*KRITNQRRDSVSEQVF WTMDWLSPSLL*KCLRATGSWWLTFSPTSFPRKD TAGVSLVMWKRTGLRPQKGLNRRWRPPVPSMAT HPGTWQTAPR*MEPLATAAVWMPGR*SPWQQ*S KR*GRQATSLNCGTGGHSVT*HPSSTSPQGQHIR ALNR**MNSSGMG*TGVALWPFSPSAGHCAWKA *TRRCRYW*VGSQLGWPLT*MTT*SLGSRRTAA G*E

Frame 2

RKQARGLVPELRNSCVAFWAPSLPGRMIPPAGA GFFASHREAG*VTGIPAATSSPDL*YKRSSGGC TCLPLPKADLNVGGAGEREWGGGDCPGSDFPRK AFRRRRG*KRLVGDSESTGAFDLT*VKYLGT*TQ TFLFLSLQNLILALDLRRESLTRDETQ*VSRCF GQWTG*AHPYYKNVSEQPGAGG*LSLLQAFPER IQLESV**CGREQD*GPRRD*IGDGDPQCHQWQP ILAPGRQPRGEWSHWPQQQFGCPGGDPHGSSKA SAEGGRRRV*TAVPAGIQ*PDIPAPHHPRDSIS EL*TGSE*TLPGWGKLGSHCGLFLLRRGTVRGKR RQGDAGIGESDRSLDGHLPE*PPRALDPGERRL GKN