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Sequence BCL2L1.cAug05
GGCTGCGCTGGGGCGGACCGCCGGGGGCGAGGCCCCTCCTCTCAGGAAGGTCTAGGGGGCGCCCCCGGGGGAGGGCGGTTGCCTAGCAACGGGGCGGTGGCTCGGCGGGTACCCGGCCTAGTGAGCTCGAGAAGCCGCTAGGCCGCAGCCGGCCCACCCTGGGCTCCGGAGAGGTGCAGCCCCCGGCCCCGGAGCCCTCC
CGGGGGCGCCAGAAAGCCACTTTGGGCCTGTCCCCAGGAAAACGTGGTCTCAGCTGTGGGGCCGTCGAGCAGGCCTCGACCCCTCTTCCTGACCTGAGGA
GAGGCCCAGCGCCTCACCCTCACCCAGTCTTTGTGCAGGGCGCCGGCGGCCATTGTGTCCGGGCGGGGAATGGAGGACCCGGCAATCCCCAACTGCCACG
TTCTGGGCCTTTGGGGAATTCAGAGCAAACCAGCGGCATTTGTTGGGGGTCTCCGGCCTTCAACATCACAGACAGGCCTGGGGGTGGCCTTCCCAAAGTC
AGATTGCAGATCTGAGGCAGTTTCCCCCTCCCTGCGTCCCTCACTGAAACCTTGAACCCCATTGAGAAGTCCCTTTAGGGTTTCGGACGCCTCCACCTCA
CCCTGGGCTGGTGCTTAAATAGAAAAAAGAAAAACAAAAACCAACTAAATCCATACCAGCCACCTCCGGGAGAGTACTCCTGGCTCCCAGTAGGAGGCGG
AGAGCCAAGGGGCGTGCAAGAGAGAGGGGGCTGGGCTCCCGGGTGGCAGGAGGCCGCGGCTGCGGAGCGGCCGCCCTCGATCCGGGCGATGGAGGAGGAA
GCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTT
TTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGCACCTGCC
TGCCTTTGCCTAAGGCGGATTTGAATAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGAGCAGGTGTTT
TGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTTCCCAGAAAGG
ATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCATCAATGGCAAC
CCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCAGCAGTAAAGC
AAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGACAGCATATCA
GAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTGCGTGGAAAGC
GTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAGAACGGCGGCT
GGGATACTTTTGTGGAACTCTATGGGAACAATGCAGCAGCCGAGAGCCGAAAGGGCCAGGAACGCTTCAACCGCTGGTTCCTGACGGGCATGACTGTGGC
CGGCGTGGTTCTGCTGGGCTCACTCTTCAGTCGGAAATGACCAGACACTGACCATCCACTCTACCCTCCCACCCCCTTCTCTGCTCCACCACATCCTCCG
TCCAGCCGCCATTGCCACCAGGAGAACCACTACATGCAGCCCATGCCCACCTGCCCATCACAGGGTTGGGCCCAGATCTGGTCCCTTGCAGCTAGTTTTC
TAGAATTTATCACACTTCTGTGAGACCCCCACACCTCAGTTCCCTTGGCCTCAGAATTCACAAAATTTCCACAAAATCTGTCCAAAGGAGGCTGGCAGGT
ATGGAAGGGTTTGTGGCTGGGGGCAGGAGGGCCCTACCTGATTGGTGCAACCCTTACCCCTTAGCCTCCCTGAAAATGTTTTTCTGCCAGGGAGCTTGAA
AGTTTTCAGAACCTCTTCCCCAGAAAGGAGACTAGATTGCCTTTGTTTTGATGTTTGTGGCCTCAGAATTGATCATTTTCCCCCCACTCTCCCCACACTA
ACCTGGGTTCCCTTTCCTTCCATCCCTACCCCCTAAGAGCCATTTAGGGGCCACTTTTGACTAGGGATTCAGGCTGCTTGGGATAAAGATGCAAGGACCA
GGACTCCCTCCTCACCTCTGGACTGGCTAGAGTCCTCACTCCCAGTCCAAATGTCCTCCAGAAGCCTCTGGCTAGAGGCCAGCCCCACCCAGGAGGGAGG
GGGCTATAGCTACAGGAAGCACCCCATGCCAAAGCTAGGGTGGCCCTTGCAGTTCAGCACCACCCTAGTCCCTTCCCCTCCCTGGCTCCCATGACCATAC
TGAGGGACCAACTGGGCCCAAGACAGATGCCCCAGAGCTGTTTATGGCCTCAGCTGCCTCACTTCCTACAAGAGCAGCCTGTGGCATCTTTGCCTTGGGC
TGCTCCTCATGGTGGGTTCAGGGGACTCAGCCCTGAGGTGAAAGGGAGCTATCAGGAACAGCTATGGGAGCCCCAGGGTCTTCCCTACCTCAGGCAGGAA
GGGCAGGAAGGAGAGCCTGCTGCATGGGGTGGGGTAGGGCTGACTAGAAGGGCCAGTCCTGCCTGGCCAGGCAGATCTGTGCCCCATGCCTGTCCAGCCT
GGGCAGCCAGGCTGCCAAGGCCAGAGTGGCCTGGCCAGGAGCTCTTCAGGCCTCCCTCTCTCTTCTGCTCCACCCTTGGCCTGTCTCATCCCCAGGGGTC
CCAGCCACCCCGGGCTCTCTGCTGTACATATTTGAGACTAGTTTTTATTCCTTGTGAAGATGATATACTATTTTTGTTAAGCGTGTCTGTATTTATGTGT
GAGGAGCTGCTGGCTTGCAGTGCGCGTGCACGTGGAGAGCTGGTGCCCGGAGATTGGACGGCCTGATGCTCCCTCCCCTGCCCTGGTCCAGGGAAGCTGG
CCGAGGGTCCTGGCTCCTGAGGGGCATCTGCCCCTCCCCCAACCCCCACCCCACACTTGTTCCAGCTCTTTGAAATAGTCTGTGTGAAGGTGAAAGTGCA
GTTCAGTAATAAACTGTGTTTACTCAGTGAACAAAGAGCC
- BCL2L1.u.f.5 (forward primer)
- BCL2L1.u.r.5 (reverse primer)
- Alternative donor
Frame 0
GCAGADRRGRGPSSQEGLGGAPGGGRLPSNGAVARRVPGLVSSRSR*AAAGPPWAPERCSPRPRSPP
GGARKPLWACPQENVVSAVGPSSRPRPLFLT*G
EAQRLTLTQSLCRAPAAIVSGRGMEDPAIPNCH
VLGLWGIQSKPAAFVGGLRPSTSQTGLGVAFPKS
DCRSEAVSPSLRPSLKP*TPLRSPFRVSDASTS
PWAGA*IEKRKTKTN*IHTSHLRESTPGSQ*EA
ESQGACKREGAGLPGGRRPRLRSGRPRSGRWRRK
QARGLVPELRNSCVAFWAPSLPGRMIPPAGAGF
FASHREAG*VTGIPAATSSPDL*YKRSSGGCTC
LPLPKADLNNLILALDLRRESLTRDETQ*VSRCF
GQWTG*AHPYYKNVSEQPGAGG*LSLLQAFPER
IQLESV**CGREQD*GPRRD*IGDGDPQCHQWQ
PILAPGRQPRGEWSHWPQQQFGCPGGDPHGSSKA
SAEGGRRRV*TAVPAGIQ*PDIPAPHHPRDSIS
EL*TGSE*TLPGWGKLGSHCGLFLLRRGTVRGK
RRQGDAGIGESDRSLDGHLPE*PPRALDPGERRL
GYFCGTLWEQCSSREPKGPGTLQPLVPDGHDCG
RRGSAGLTLQSEMTRH*PSTLPSHPLLCSTTSS
VQPPLPPGEPLHAAHAHLPITGLGPDLVPCS*FS
RIYHTSVRPPHLSSLGLRIHKISTKSVQRRLAG
MEGFVAGGRRALPDWCNPYPLASLKMFFCQGA*
KFSEPLPQKGD*IAFVLMFVASELIIFPPLSPH*
PGFPFLPSLPPKSHLGATFD*GFRLLGIKMQGP
GLPPHLWTG*SPHSQSKCPPEASG*RPAPPRRE
GAIATGSTPCQS*GGPCSSAPP*SLPLPGSHDHT
EGPTGPKTDAPELFMASAASLPTRAACGIFALG
CSSWWVQGTQP*GERELSGTAMGAPGSSLPQAG
RAGRRACCMGWGRAD*KGQSCLARQICAPCLSSL
GSQAAKARVAWPGALQASLSLLLHPWPVSSPGV
PATPGSLLYIFETSFYSL*R*YTIFVKRVCIYV
*GAAGLQCACTWRAGARRLDGLMLPPLPWSREAG
RGSWLLRGICPSPNPHPTLVPAL*NSLCEGESA
VQ**TVFTQ*TKS
Frame 1
AALGRTAGGEAPPLRKV*GAPPGEGGCLATGRWLGGYPA**AREAARPQPAHPGLRRGAAPGPGAL
PGAPESHFGPVPRKTWSQLWGRRAGLDPSS*PEE
RPSASPSPSLCAGRRRPLCPGGEWRTRQSPTAT
FWAFGEFRANQRHLLGVSGLQHHRQAWGWPSQS
QIADLRQFPPPCVPH*NLEPH*EVPLGFRTPPPH
PGLVLK*KKEKQKPTKSIPATSGRVLLAPSRRR
RAKGRARERGLGSRVAGGRGCGAAALDPGDGGG
SKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVF
LPATARPAELPASPQPPPLPTCDTKDLPGAAPA
CLCLRRI*IILSWLWILEENH*PETRLSE*AGV
LDNGLVEPIPIIKMSQSNRELVVDFLSYKLSQKG
YSWSQFSDVEENRTEAPEGTESEMETPSAINGN
PSWHLADSPAVNGATGHSSSLDAREVIPMAAVK
QALREAGDEFELRYRRAFSDLTSQLHITPGTAYQ
SFEQVVNELFRDGVNWGRIVAFFSFGGALCVES
VDKEMQVLVSRIAAWMATYLNDHLEPWIQENGG
WDTFVELYGNNAAAESRKGQERFNRWFLTGMTVA
GVVLLGSLFSRK*PDTDHPLYPPTPFSAPPHPP
SSRHCHQENHYMQPMPTCPSQGWAQIWSLAASF
LEFITLL*DPHTSVPLASEFTKFPQNLSKGGWQV
WKGLWLGAGGPYLIGATLTP*PP*KCFSARELE
SFQNLFPRKETRLPLF*CLWPQN*SFSPHSPHT
NLGSLSFHPYPLRAI*GPLLTRDSGCLG*RCKDQ
DSLLTSGLARVLTPSPNVLQKPLARGQPHPGGR
GL*LQEAPHAKARVALAVQHHPSPFPSLAPMTI
LRDQLGPRQMPQSCLWPQLPHFLQEQPVASLPWA
APHGGFRGLSPEVKGSYQEQLWEPQGLPYLRQE
GQEGEPAAWGGVGLTRRASPAWPGRSVPHACPA
WAARLPRPEWPGQELFRPPSLFCSTLGLSHPQGS
QPPRALCCTYLRLVFIPCEDDILFLLSVSVFMC
EELLACSARARGELVPGDWTA*CSLPCPGPGKL
AEGPGS*GASAPPPTPTPHLFQLFEIVCVKVKVQ
FSNKLCLLSEQRA
Frame 2
LRWGGPPGARPLLSGRSRGRPRGRAVA*QRGGGSAGTRPSELEKPLGRSRPTLGSGEVQPPAPEPS
RGRQKATLGLSPGKRGLSCGAVEQASTPLPDLR
RGPAPHPHPVFVQGAGGHCVRAGNGGPGNPQLPR
SGPLGNSEQTSGICWGSPAFNITDRPGGGLPKV
RLQI*GSFPLPASLTETLNPIEKSL*GFGRLHL
TLGWCLNRKKKNKNQLNPYQPPPGEYSWLPVGGG
EPRGVQERGGWAPGWQEAAAAERPPSIRAMEEE
ASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWF
FCQPPRGRLSYRHPRSHLLSRPVIQKIFRGLHLP
AFA*GGFE*SYLGFGS*KRITNQRRDSVSEQVF
WTMDWLSPSLL*KCLRATGSWWLTFSPTSFPRK
DTAGVSLVMWKRTGLRPQKGLNRRWRPPVPSMAT
HPGTWQTAPR*MEPLATAAVWMPGR*SPWQQ*S
KR*GRQATSLNCGTGGHSVT*HPSSTSPQGQHI
RALNR**MNSSGMG*TGVALWPFSPSAGHCAWKA
*TRRCRYW*VGSQLGWPLT*MTT*SLGSRRTAA
GILLWNSMGTMQQPRAERARNASTAGS*RA*LW
PAWFCWAHSSVGNDQTLTIHSTLPPPSLLHHILR
PAAIATRRTTTCSPCPPAHHRVGPRSGPLQLVF
*NLSHFCETPTPQFPWPQNSQNFHKICPKEAGR
YGRVCGWGQEGPT*LVQPLPLSLPENVFLPGSLK
VFRTSSPERRLDCLCFDVCGLRIDHFPPTLPTL
TWVPFPSIPTP*EPFRGHF*LGIQAAWDKDART
RTPSSPLDWLESSLPVQMSSRSLWLEASPTQEGG
GYSYRKHPMPKLGWPLQFSTTLVPSPPWLP*PY
*GTNWAQDRCPRAVYGLSCLTSYKSSLWHLCLG
LLLMVGSGDSALR*KGAIRNSYGSPRVFPTSGRK
GRKESLLHGVG*G*LEGPVLPGQADLCPMPVQP
GQPGCQGQSGLARSSSGLPLSSAPPLACLIPRG
PSHPGLSAVHI*D*FLFLVKMIYYFC*ACLYLCV
RSCWLAVRVHVESWCPEIGRPDAPSPALVQGSW
PRVLAPEGHLPLPQPPPHTCSSSLK*SV*R*KC
SSVINCVYSVNKE
Sequence BCL2L1.gAug05
GGAGGAAGCAAGCGAGGGGGCTGGTTCCTGAGCTTCGCAATTCCTGTGTCGCCTTCTGGGCTCCCAGCCTGCCGGGTCGCATGATCCCTCCGGCCGGAGCTGGTTTTTTTGCCAGCCACCGCGAGGCCGGCTGAGTTACCGGCATCCCCGCAGCCACCTCCTCTCCCGACCTGTGATACAAAAGATCTTCCGGGGGCTGC
ACCTGCCTGCCTTTGCCTAAGGCGGATTTGAATGTAGGTGGTGCGGGGGAGCGGGAGTGGGGCGGGGGGGACTGCCCAGGGAGTGACTTTCCGAGGAAGG
CATTTCGGAGAAGACGGGGGTAGAAAAGGCTGGTGGGAGATTCAGAGTCCACTGGTGCTTTCGATTTGACTTAAGTGAAGTATCTTGGAACCTAGACCCA
GACCTTCGTAAGACCCACAAAGAAACCAGTTCTGAATCTTATCTTGGCTTTGGATCTTAGAAGAGAATCACTAACCAGAGACGAGACTCAGTGAGTGAGC
AGGTGTTTTGGACAATGGACTGGTTGAGCCCATCCCTATTATAAAAATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTTTCC
CAGAAAGGATACAGCTGGAGTCAGTTTAGTGATGTGGAAGAGAACAGGACTGAGGCCCCAGAAGGGACTGAATCGGAGATGGAGACCCCCAGTGCCATCA
ATGGCAACCCATCCTGGCACCTGGCAGACAGCCCCGCGGTGAATGGAGCCACTGGCCACAGCAGCAGTTTGGATGCCCGGGAGGTGATCCCCATGGCAGC
AGTAAAGCAAGCGCTGAGGGAGGCAGGCGACGAGTTTGAACTGCGGTACCGGCGGGCATTCAGTGACCTGACATCCCAGCTCCACATCACCCCAGGGACA
GCATATCAGAGCTTTGAACAGGTAGTGAATGAACTCTTCCGGGATGGGGTAAACTGGGGTCGCATTGTGGCCTTTTTCTCCTTCGGCGGGGCACTGTGCG
TGGAAAGCGTAGACAAGGAGATGCAGGTATTGGTGAGTCGGATCGCAGCTTGGATGGCCACTTACCTGAATGACCACCTAGAGCCTTGGATCCAGGAGAA
CGGCGGCTGGGTAAGAACC
- BCL2L1.u.f.5 (forward primer)
- BCL2L1.u.r.5 (reverse primer)
- Alternative donor
Frame 0
GGSKRGGWFLSFAIPVSPSGLPACRVA*SLRPELVFLPATARPAELPASPQPPPLPTCDTKDLPGAA
PACLCLRRI*M*VVRGSGSGAGGTAQGVTFRGR
HFGEDGGRKGWWEIQSPLVLSI*LK*SILEPRP
RPS*DPQRNQF*ILSWLWILEENH*PETRLSE*A
GVLDNGLVEPIPIIKMSQSNRELVVDFLSYKLS
QKGYSWSQFSDVEENRTEAPEGTESEMETPSAI
NGNPSWHLADSPAVNGATGHSSSLDAREVIPMAA
VKQALREAGDEFELRYRRAFSDLTSQLHITPGT
AYQSFEQVVNELFRDGVNWGRIVAFFSFGGALC
VESVDKEMQVLVSRIAAWMATYLNDHLEPWIQEN
GGWVRT
Frame 1
EEASEGAGS*ASQFLCRLLGSQPAGSHDPSGRSWFFCQPPRGRLSYRHPRSHLLSRPVIQKIFRGL
HLPAFA*GGFECRWCGGAGVGRGGLPRE*LSEEG
ISEKTGVEKAGGRFRVHWCFRFDLSEVSWNLDP
DLRKTHKETSSESYLGFGS*KRITNQRRDSVSE
QVFWTMDWLSPSLL*KCLRATGSWWLTFSPTSFP
RKDTAGVSLVMWKRTGLRPQKGLNRRWRPPVPS
MATHPGTWQTAPR*MEPLATAAVWMPGR*SPWQ
Q*SKR*GRQATSLNCGTGGHSVT*HPSSTSPQGQ
HIRALNR**MNSSGMG*TGVALWPFSPSAGHCA
WKA*TRRCRYW*VGSQLGWPLT*MTT*SLGSRR
TAAG*E
Frame 2
RKQARGLVPELRNSCVAFWAPSLPGRMIPPAGAGFFASHREAG*VTGIPAATSSPDL*YKRSSGGC
TCLPLPKADLNVGGAGEREWGGGDCPGSDFPRK
AFRRRRG*KRLVGDSESTGAFDLT*VKYLGT*TQ
TFVRPTKKPVLNLILALDLRRESLTRDETQ*VS
RCFGQWTG*AHPYYKNVSEQPGAGG*LSLLQAF
PERIQLESV**CGREQD*GPRRD*IGDGDPQCHQ
WQPILAPGRQPRGEWSHWPQQQFGCPGGDPHGS
SKASAEGGRRRV*TAVPAGIQ*PDIPAPHHPRD
SISEL*TGSE*TLPGWGKLGSHCGLFLLRRGTVR
GKRRQGDAGIGESDRSLDGHLPE*PPRALDPGE
RRLGKN
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