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Sequences

Sequence AXIN1.bAug05

CTCGGGAGGCGATCGTGGCCGCTGCCGCCGCCAGAGCCGGCCGGAGTCGGCCCGGAGCCGCGTCCACGCGGAGCGGGCGGGTGGGAGCTGGCGGCTGGGC
CCCTGCGGCGCCCCCGCGGCCTGCCCCGCGTCCGCCTCCGGGCCGCCGAGCCGCAGCCGCCGAGATGGGGGCCGCCCCGGGCCGCGCCCCCGCCGGGTCC
CGCCCGCCGCGCTGCCGCTGAGCGCATGGGCCCGGACCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGTCCCGCCACCACCGCGCGCGGGACAGATTG
ATTCACTTTGGAGCTGTAAGTACTGATGTATTAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTTCCCCT
TGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAG
TTTCTGCTCCGGGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCC
TCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGG
GCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGC
CATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTG
ATCGATCCTGCCATGTTTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAAT
ATACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGA
AGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCT
GCCCCGAGGGTCTCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCAATGCCGGCT
ATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGAT
CCCCCCATACAGGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCACGTACCGG
GTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGG
AGGAGCGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGC
TTGGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCCTGAGAGCATCCTGGACGAGCACGTACAG
CGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCG
CCTCGGGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCAC
AGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGA
GGCTACTCAGAGAGTGTTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTG
AGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGAT
CAGCAGGCACCGCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGC
CCTCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGG
CGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCC
GTGTGACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAG
CTGCTGACCAAAAAGGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCG
TCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACTGATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTTGGCGGGCA
CGGGTGTCACGGCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCCATCCCTGTGGTCTACCCGTGTCTAGAGGC
AGGTAGGGGGTCCCTCCAAGTGGTCCACAAGCTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGCCCAGCCCAA
GTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCCCCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTCCCTCTGGG
ATTTGCCCACGTACCCAGGTCTGGGTGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATTGTACGCGCCACCGAGTGCCTTCAACACAG
CTTGTCTCTTGCCTGCCACTGTGTGAATCGGCGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTGAGGGCCCGT
GTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCGAGTCTGTTTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTT
GGATAATAATGAAATAAAACTGTTTTTGAACCTGC
  • AXIN1.u.f.14 (forward primer)
  • AXIN1.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

LGRRSWPLPPPEPAGVGPEPRPRGAGGWELAAG
PLRRPRGLPRVRLRAAEPQPPRWGPPRAAPPPGP
ARRAAAERMGPDRAAPLREPGPGSRHHRARDRL
IHFGAVSTDVLGCSAHCSLTQSPKMNIQEQGFP
LDLGASFTEDAPRPPVPGEEGELVSTDPRPASYS
FCSGKGVGIKGETSTATPRRSDLDLGYEPEGSA
SPTPPYLKWAESLHSLLDDQDGISLFRTFLKQE
GCADLLDFWFACTGFRKLEPCDSNEEKRLKLARA
IYRKYILDNNGIVSRQTKPATKSFIKGCIMKQL
IDPAMFDQAQTEIQATMEENTYPSFLKSDIYLE
YTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNE
DEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETA
APRVSSSRRYSEGREFRYGSWREPVNPYYVNAG
YALAPATSANDSEQQSLSSDADTLSLTDSSVDGI
PPYRIRKQHRREMQESVQVNGRVPLPHIPRTYR
VPKEVRVEPQKFAEELIHRLEAVQRTREAEEKL
EERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPA
WHHFPPRCVDMGCAGLRDAHEENPESILDEHVQ
RVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGA
ASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHST
ARPKEQVEAEATRRAQSSFAWGLEPHSHGARSR
GYSESVGAAPNASDGLAHSGKVGVACKRNAKKA
ESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEI
SRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAG
PQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEE
ARRRLEEEEKRASRAPSKQRTRSQRKVGGGSAQP
CDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKE
LLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEA
VLPVFEEKIIGKVEKVD**AGGLAAVPGEALGGH
GCHGQADDLVLRSPMGNSVGCTTHPCGLPVSRG
R*GVPPSGPQASVLPPRRQPGPLLIAILGGPSP
SEAAEVPAASFR*PPPIPRHLPWARVLGSTFPLG
FAHVPRSGWGPGPDAEACRASVNCTRHRVPSTQ
LVSCLPLCESATEHCTCLQPPAVQSWVLLSEGP
CKYVHFSG*ASRGCPSLFFMR*HLYN*LSFQRYL
DNNEIKLFLNL

Frame 1

SGGDRGRCRRQSRPESARSRVHAERAGGSWRLG
PCGAPAACPASASGPPSRSRRDGGRPGPRPRRV
PPAALPLSAWARTAPRRSGSRARGPATTARGTD*
FTLEL*VLMY*GAALIVP*RRVPK*ISKSRVSP
WTSEQVSPKMLPDPQCLVRRENWCPQTRGPPAT
VSAPGKVLALKVRLRRPLRGARIWTWGMSLRAVP
PPPHHT*SGLSHCIPCWMTKMG*ACSGLS*SRR
AVPTCWTSGLPALASGSWSPVTRTRRRG*SWRE
PSTESTFLITMASCPGRPSQPPRAS*RAAS*SS*
SILPCLTRPRPKSRPLWRKTPIPPSLSLIFIWN
IRGQARRAPKSVVTRALGQGQGRAYLDTCRP*M
KMRNGSVTRTWMRTMAETLLPPEDSLRSCSWRQL
PRGSPPVDGTAKAESSGMDPGGSQSTPIMSMPA
MPWPQPPVPTTASSRACPAMQTPCPSRTAAWMG
SPHTGSVSSTAGRCRRACRSMGGCPYLTFPARTG
CRRRSAWSLRSSRRSSSTAWRLCSARGRPRRSW
RSG*SACAWRRKVRTAIHRQGPQGRVTSCLPPP
LGTTSRPAVWTWAVPGSGMHTRRTLRASWTSTYS
VC*GHLAASRLGLAIAPRTVGTWPRCQWHWGVP
PRGTGSTYPSQGRSWTRPACTTTDTSTTTSTTA
QPGPRSRWRPRPPAGPRAASPGAWNHTAMGQGPE
ATQRVLALPPTPVMASPTVGRWAWRAKEMPRRL
SRGRAPAPRCQVPRRMRRRTRKSCSGSLRGKRR
SAGTAGPATGLRGRGSHSPMRTPDPCPLSTPGPA
LSSGPPCSPPTSSSKTPPCHPTQLPTP*PSWRR
RADVWRRKKREPAEHPPSRGQDRRGRWAAGVPS
RVTASLWRTTSAGNPSPTAPW*GAALSPWASSRS
C*PKRAATDTTSRK*ATSLTVGWCLRRFERTRP
SCPSLRRRSSAKWRRWTDRLVGWPLCQARPLAG
TGVTARQMTSYSGARWGTVLGVPPIPVVYPCLEA
GRGSLQVVHKLLSCPQGGSLDHSS*QYLEGPAQ
VRQPRSLLPASGDPPPSPGTSLGHVCWDLLSLW
DLPTYPGLGGAQARMQRPAGPLSIVRATECLQHS
LSLACHCVNRRRSTAPASSRRLCSPGSSFLRAR
VNMYISQARPAGAARVCFSCDDTCTINYLFKGT
WIIMK*NCF*TC

Frame 2

REAIVAAAAARAGRSRPGAASTRSGRVGAGGWA
PAAPPRPAPRPPPGRRAAAAEMGAAPGRAPAGS
RPPRCR*AHGPGPRRAAPGAGPGVPPPPRAGQI
DSLWSCKY*CIRVQRSLFLDAESQNEYPRAGFPL
GPRSKFHRRCSPTPSAW*GGRTGVHRPEARQLQ
FLLRERCWH*R*DFDGHSEALGSGPGV*A*GQC
LPHPTILEVG*VTAFPAG*PRWDKPVQDFPEAGG
LCRLAGLLVCLHWLQEAGAL*LERGEEAEAGES
HLPKVHS**QWHRVPADQASHQELHKGLHHEAA
DRSCHV*PGPDRNPGHYGGKHLSLLP*V*YLFGI
YEDRLGEPQSL**PELWVRDREGHIWIPADLK*
R*GMEV*PGHG*GRWQRRCSPRKTPSEAAPGDS
CPEGLLQ*TVQRRQRVQVWILAGASQPLLCQCRL
CPGPSHQCQRQRAAEPVQRCRHPVPHGQQRGWD
PPIQDP*AAPQGDAGERAGQWAGAPTSHSPHVP
GAEGGPRGASEVRGGAHPPPGGCAAHAGGRGEAG
GAAEARAHGGGR*GRRSIVRAPRAVSQAASRPR
LAPLPAPLCGHGLCRAPGCTRGEP*EHPGRART
ACAEDTWPPVAWAWPSLPGQWARGQDASGTGGCR
LGAREARTQVRGEAGRGRPAPPPTRPPPRPPQH
SPAQGAGGGRGHPQGPEQLRLGPGTTQPWGKVP
RLLRECWRCPQRQ*WPRPQWEGGRGVQKKCQEG*
VGEERQHRGARCLGGCGEEPENHAVDH*GGKGD
QQAPQDRPRVFGDEEATAP*ELQTLVP*APLGR
PSAPDLRAALPPLHPRPHHATPPSSQPPNPAGGG
APTSGGGRKESQPSTLQAEDKIAEEGGRRECPA
V*QHRCGVLLLRGTHPLPHPGEGPRCHPGPVQG
AADQKGQLQILLQESERRV*LWGGV*GGSRGRGR
PARL*GEDHRQSGEGGLIGWWAGRCARRGPWRA
RVSRPGR*PRTQEPDGEQCWVYHPSLWSTRV*R
QVGGPSKWSTSFCPAPKEAAWTTPHSNTWRAQPK
*GSRGPCCQLQVTPPHPPAPPLGTCAGIYFPSG
ICPRTQVWVGPRPGCRGLQGLCQLYAPPSAFNT
ACLLPATV*IGDGALHLPPAAGCAVLGPPF*GPV
*ICTFLRLGQQGLPESVFHAMTLVQLIIFSKVL
G***NKTVFEP

Sequence AXIN1.eAug05

GTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAG
CATCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACC
AAAAAGGGCAGCTACAGGCCTCCTTCCCATCTGCGGTGCTGCCTGCTCACTGCCCTCCTCACCTCCGTCCTGCAGATACTACTTCAAGAAAGTGAGCGAC
GAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTC
  • AXIN1.u.f.14 (forward primer)
  • AXIN1.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

VWRRKKREPAEHPPSRGQDRRGRWAAGVPSRVT
ASLWRTTSAGNPSPTAPW*GAALSPWASSRSC*P
KRAATGLLPICGAACSLPSSPPSCRYYFKKVSD
EFDCGVVFEEV

Frame 1

SGGGRKESQPSTLQAEDKIAEEGGRRECPAV*Q
HRCGVLLLRGTHPLPHPGEGPRCHPGPVQGAAD
QKGQLQASFPSAVLPAHCPPHLRPADTTSRK*AT
SLTVGWCLRRF

Frame 2

LEEEEKRASRAPSKQRTRSQRKVGGGSAQPCDS
IVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLT
KKGSYRPPSHLRCCLLTALLTSVLQILLQESER
RV*LWGGV*GG

Sequence AXIN1.aAug05

CTCGGGAGGCGATCGTGGCCGCTGCCGCCGCCAGAGCCGGCCGGAGTCGGCCCGGAGCCGCGTCCACGCGGAGCGGGCGGGTGGGAGCTGGCGGCTGGGC
CCCTGCGGCGCCCCCGCGGCCTGCCCCGCGTCCGCCTCCGGGCCGCCGAGCCGCAGCCGCCGAGATGGGGGCCGCCCCGGGCCGCGCCCCCGCCGGGTCC
CGCCCGCCGCGCTGCCGCTGAGCGCATGGGCCCGGACCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGTCCCGCCACCACCGCGCGCGGGACAGATTG
ATTCACTTTGGAGCTGTAAGTACTGATGTATTAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTTCCCCT
TGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAG
TTTCTGCTCCGGGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCC
TCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGG
GCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGC
CATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTG
ATCGATCCTGCCATGTTTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAAT
ATACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGA
AGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCT
GCCCCGAGGGTCTCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCAATGCCGGCT
ATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGAT
CCCCCCATACAGGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCACGTACCGG
GTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGG
AGGAGCGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGC
TTGGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCCTGAGAGCATCCTGGACGAGCACGTACAG
CGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCG
CCTCGGGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCAC
AGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGA
GGCTACTCAGAGAGTGTTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTG
AGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGAT
CAGCAGGCACCGCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGC
CCTCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGG
CGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCGGCGGGGACGCGCCTGCGTCAG
GCCAGCGTGCGCGCCGGTGCTGCACGTGGTACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGT
GCCCAGCCGTGTGACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGT
TCAAGGAGCTGCTGACCAAAAAGGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGGA
CGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACTGATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTT
GGCGGGCACGGGTGTCACGGCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCCATCCCTGTGGTCTACCCGTGT
CTAGAGGCAGGTAGGGGGTCCCTCCAAGTGGTCCACAAGCTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGCC
CAGCCCAAGTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCCCCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTC
CCTCTGGGATTTGCCCACGTACCCAGGTCTGGGTGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATTGTACGCGCCACCGAGTGCCTT
CAACACAGCTTGTCTCTTGCCTGCCACTGTGTGAATCGGCGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTGA
GGGCCCGTGTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCGAGTCTGTTTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAA
AGGTACTTGGATAATAATGAAATAAAACTGTTTTTGAACCTGC
  • AXIN1.u.f.14 (forward primer)
  • AXIN1.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

LGRRSWPLPPPEPAGVGPEPRPRGAGGWELAAG
PLRRPRGLPRVRLRAAEPQPPRWGPPRAAPPPGP
ARRAAAERMGPDRAAPLREPGPGSRHHRARDRL
IHFGAVSTDVLGCSAHCSLTQSPKMNIQEQGFP
LDLGASFTEDAPRPPVPGEEGELVSTDPRPASYS
FCSGKGVGIKGETSTATPRRSDLDLGYEPEGSA
SPTPPYLKWAESLHSLLDDQDGISLFRTFLKQE
GCADLLDFWFACTGFRKLEPCDSNEEKRLKLARA
IYRKYILDNNGIVSRQTKPATKSFIKGCIMKQL
IDPAMFDQAQTEIQATMEENTYPSFLKSDIYLE
YTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNE
DEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETA
APRVSSSRRYSEGREFRYGSWREPVNPYYVNAG
YALAPATSANDSEQQSLSSDADTLSLTDSSVDGI
PPYRIRKQHRREMQESVQVNGRVPLPHIPRTYR
VPKEVRVEPQKFAEELIHRLEAVQRTREAEEKL
EERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPA
WHHFPPRCVDMGCAGLRDAHEENPESILDEHVQ
RVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGA
ASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHST
ARPKEQVEAEATRRAQSSFAWGLEPHSHGARSR
GYSESVGAAPNASDGLAHSGKVGVACKRNAKKA
ESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEI
SRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAG
PQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEE
ARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVR
PACAPVLHVVPAVSDMELSETETRSQRKVGGGS
AQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQ
FKELLTKKGSYRYYFKKVSDEFDCGVVFEEVRED
EAVLPVFEEKIIGKVEKVD**AGGLAAVPGEAL
GGHGCHGQADDLVLRSPMGNSVGCTTHPCGLPV
SRGR*GVPPSGPQASVLPPRRQPGPLLIAILGGP
SPSEAAEVPAASFR*PPPIPRHLPWARVLGSTF
PLGFAHVPRSGWGPGPDAEACRASVNCTRHRVP
STQLVSCLPLCESATEHCTCLQPPAVQSWVLLSE
GPCKYVHFSG*ASRGCPSLFFMR*HLYN*LSFQ
RYLDNNEIKLFLNL

Frame 1

SGGDRGRCRRQSRPESARSRVHAERAGGSWRLG
PCGAPAACPASASGPPSRSRRDGGRPGPRPRRV
PPAALPLSAWARTAPRRSGSRARGPATTARGTD*
FTLEL*VLMY*GAALIVP*RRVPK*ISKSRVSP
WTSEQVSPKMLPDPQCLVRRENWCPQTRGPPAT
VSAPGKVLALKVRLRRPLRGARIWTWGMSLRAVP
PPPHHT*SGLSHCIPCWMTKMG*ACSGLS*SRR
AVPTCWTSGLPALASGSWSPVTRTRRRG*SWRE
PSTESTFLITMASCPGRPSQPPRAS*RAAS*SS*
SILPCLTRPRPKSRPLWRKTPIPPSLSLIFIWN
IRGQARRAPKSVVTRALGQGQGRAYLDTCRP*M
KMRNGSVTRTWMRTMAETLLPPEDSLRSCSWRQL
PRGSPPVDGTAKAESSGMDPGGSQSTPIMSMPA
MPWPQPPVPTTASSRACPAMQTPCPSRTAAWMG
SPHTGSVSSTAGRCRRACRSMGGCPYLTFPARTG
CRRRSAWSLRSSRRSSSTAWRLCSARGRPRRSW
RSG*SACAWRRKVRTAIHRQGPQGRVTSCLPPP
LGTTSRPAVWTWAVPGSGMHTRRTLRASWTSTYS
VC*GHLAASRLGLAIAPRTVGTWPRCQWHWGVP
PRGTGSTYPSQGRSWTRPACTTTDTSTTTSTTA
QPGPRSRWRPRPPAGPRAASPGAWNHTAMGQGPE
ATQRVLALPPTPVMASPTVGRWAWRAKEMPRRL
SRGRAPAPRCQVPRRMRRRTRKSCSGSLRGKRR
SAGTAGPATGLRGRGSHSPMRTPDPCPLSTPGPA
LSSGPPCSPPTSSSKTPPCHPTQLPTP*PSWRR
RADVWRRKKREPAEHPPSRGMCRRLCGGDAPAS
GQRARRCCTWYQPCRTWSSPRQRQDRRGRWAAGV
PSRVTASLWRTTSAGNPSPTAPW*GAALSPWAS
SRSC*PKRAATDTTSRK*ATSLTVGWCLRRFER
TRPSCPSLRRRSSAKWRRWTDRLVGWPLCQARPL
AGTGVTARQMTSYSGARWGTVLGVPPIPVVYPC
LEAGRGSLQVVHKLLSCPQGGSLDHSS*QYLEG
PAQVRQPRSLLPASGDPPPSPGTSLGHVCWDLLS
LWDLPTYPGLGGAQARMQRPAGPLSIVRATECL
QHSLSLACHCVNRRRSTAPASSRRLCSPGSSFL
RARVNMYISQARPAGAARVCFSCDDTCTINYLFK
GTWIIMK*NCF*TC

Frame 2

REAIVAAAAARAGRSRPGAASTRSGRVGAGGWA
PAAPPRPAPRPPPGRRAAAAEMGAAPGRAPAGS
RPPRCR*AHGPGPRRAAPGAGPGVPPPPRAGQI
DSLWSCKY*CIRVQRSLFLDAESQNEYPRAGFPL
GPRSKFHRRCSPTPSAW*GGRTGVHRPEARQLQ
FLLRERCWH*R*DFDGHSEALGSGPGV*A*GQC
LPHPTILEVG*VTAFPAG*PRWDKPVQDFPEAGG
LCRLAGLLVCLHWLQEAGAL*LERGEEAEAGES
HLPKVHS**QWHRVPADQASHQELHKGLHHEAA
DRSCHV*PGPDRNPGHYGGKHLSLLP*V*YLFGI
YEDRLGEPQSL**PELWVRDREGHIWIPADLK*
R*GMEV*PGHG*GRWQRRCSPRKTPSEAAPGDS
CPEGLLQ*TVQRRQRVQVWILAGASQPLLCQCRL
CPGPSHQCQRQRAAEPVQRCRHPVPHGQQRGWD
PPIQDP*AAPQGDAGERAGQWAGAPTSHSPHVP
GAEGGPRGASEVRGGAHPPPGGCAAHAGGRGEAG
GAAEARAHGGGR*GRRSIVRAPRAVSQAASRPR
LAPLPAPLCGHGLCRAPGCTRGEP*EHPGRART
ACAEDTWPPVAWAWPSLPGQWARGQDASGTGGCR
LGAREARTQVRGEAGRGRPAPPPTRPPPRPPQH
SPAQGAGGGRGHPQGPEQLRLGPGTTQPWGKVP
RLLRECWRCPQRQ*WPRPQWEGGRGVQKKCQEG*
VGEERQHRGARCLGGCGEEPENHAVDH*GGKGD
QQAPQDRPRVFGDEEATAP*ELQTLVP*APLGR
PSAPDLRAALPPLHPRPHHATPPSSQPPNPAGGG
APTSGGGRKESQPSTLQAEVCAGGYAAGTRLRQ
ASVRAGAARGTSRVGHGALRDRDKIAEEGGRRE
CPAV*QHRCGVLLLRGTHPLPHPGEGPRCHPGPV
QGAADQKGQLQILLQESERRV*LWGGV*GGSRG
RGRPARL*GEDHRQSGEGGLIGWWAGRCARRGP
WRARVSRPGR*PRTQEPDGEQCWVYHPSLWSTRV
*RQVGGPSKWSTSFCPAPKEAAWTTPHSNTWRA
QPK*GSRGPCCQLQVTPPHPPAPPLGTCAGIYF
PSGICPRTQVWVGPRPGCRGLQGLCQLYAPPSAF
NTACLLPATV*IGDGALHLPPAAGCAVLGPPF*
GPV*ICTFLRLGQQGLPESVFHAMTLVQLIIFS
KVLG***NKTVFEP

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