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Sequences

Sequence APLP2.fAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCAC
TGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAA
GACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGG
AGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTC
AGACAAGGAAATTACTCATGATGTCAAAGTTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCAT
GCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGA
ACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGA
GACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCAT
CGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAA
AGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGC
CCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGG
GTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATG
GGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTT
CAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTG
GTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATC
CAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGC
AGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTG
ACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATATA
TGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCT
GACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTT
CAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATA
GGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGT
GTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGC
TTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCA
CTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACC
CCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAA
AGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTA
CAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTG
TTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATATGA
TCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHV*LRE*PYIATACCSHVG*TSSMA
LNMCAALRQRLLDLCQKKRKRKMKRKRKRKMKRK
TMMFIKVNFLLKQIWKTSQKQLWMRMMRMRKKG
RKWWRTEITTMTPSKEMTTMRRILLNPAATAPC
QTRKLLMMSKFLQLLCQPMMLMCISRPLQMIMSM
LASRRLRSSWRFGTATEWTG*RRNGKRQSFKLR
TSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWW
RPTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGLI
AFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQK
RRPR*NPR**HIST*LKKGGTKASLCSTKYLM*
PKKFKRKLMSSFRSSVQIWTSSLPQSQRPLWTSG
*ALRRVRRSHRSTPSTPSQPYLRTKDLEWESRM
GD*SVPKRK*LTVRIKWMKTWSLTRLWMLRK*F
SMPRELEASRKSGNPWAHCGRTSV*VAVLSLACW
SSQWPLPRSSSSAW*C*GRGSMAPSATGSWRLI
QCSPQKSVT*TRCRTMAMRTPPTNTWSRCRFRW
QGARQPWRRDAGGPEDPTIPIDCQAAAAARGCV*
HPDLLDCRTI*STTVELQFPFF*MGEKW*YNNI
*YINLK*KK*SIADI*CSFLF*INQKPHFHCIV
*HMLSIYNKWEMSIFNNRLICRLSFRLCCVSQLF
SLIHCPGFYLKKKKAVFPF*MSFQEVAEKWGGI
GNCNGH*ST*ETLAK*CERTSFLKSSVSTAGYL
CVSIKVLSPRTLKAFYSSVF*RA*CIQMRVSAAA
FLRNPVLPQRLACSLYLTFFTVCCLPTVAFYRS
LPLPTMSRCAGSSSLDFLQRH*PSASTLSPHLH
PLLSPAL*HSLREDHTKEAAAGPGENTGRFVCVK
GK*SRLSLKLSLWTLWKALNNCVFVTGVFVMLV
QLMSMLTASAFSFFLF*I*RFW*PVVYFYFPVT
VFVLFFSFFLPFDPIHVSTHYAQIKLHLQTP*YD
LWRMYTV*THQ*IL*LPPR

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICMSDSGNDLI*LRHAAPMWGRPVPWH
*ICVLPSDKDYWICVKRRGRGR*RGRGRGR*RG
RL*CL*K*ISY*SRSGRLHRSSCG*G**G*GRRG
GSGGGPRLLL*HLQRR*LQ*GESY*TRQRRHHV
RQGNYS*CQSSSNSSANQ*C*CVFRDLCR***A
CSLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*E
PPQSREADSDSALPSHG*SFREGSSQ*EAAAGG
DPPGPSGSYAE*PPSDGSGELPGCLAV*PATAS
SHSPGLTALCPC*EQRSLTYHPSLPACVGC*PRK
GGPDEIPGDDTSPRD*RKEEPKPLSALQSTLCS
PRNSRGN**APSGAACRYGPVHCLNLRDPCGRP
GEL*GE*GDPTVPPLPPLPSPT*ERRIWSGRAGW
GTDRCRRESD*Q*E*SG*KHGH*RDSGC*GNDF
QCRESWRPRGRAGIRGPTAGGLQSE*QCSHWPA
GHRSGHCHGHRHQPGDAEEEAVWHHQPRDRGG*S
NAHPRRASPEQDAEPWL*EPHLQIPGADADLGG
RERGSPGGGMQVGRKIPRFRSTAKQQPLPGAAS
DILTSWTVGLYKVLL*NCNFHSFKWVKNGNITIY
DI*TLNEKNDLLQIFDVVFFFKLIRNPTSIVLS
DTCSQYIINGKCRFSIIDLYAGCRSGYVV*VNS
SASFTVLAFI*RKKRQYSLFK*AFRKLLRNGVE*
GTVMATEARERPSQNDVKGPVS*SPVFPRLDTC
VSP*KSCHQGR*RHFIPASSRELSVYR*GCPLL
LSFGIQCFHRD*PVAYI*HSSLSVVYLP*LFTVH
FPFQLCPDVQAPPLWTFSKGTDPRPLLCPLTST
PSCHRPCDIHSEKTTPRRRPLAQERTRGGLFV*
KESSPGCP*N*VCGHCGKL*TIVFSSQESL*CLY
S*CRCSLLLLFLSFYFKSEGSGNLWCIFIFL*L
FLFCFFPFSSPLTLFMSLPTMHRLNFTYKLLNM
ICGECTQFKHINKYFNFHRD

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACLTQGMTLYSYGMLLPCGVDQFHGT
EYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEE
DYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEG
EEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMS
DKEITHDVKVPPTPLPTNDVDVYFETSADDNEH
ARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAK
NLPKAERQTLIQHFQAMVKALEKEAASEKQQLVE
THLARVEAMLNDRRRMALENYLAALQSDPPRPH
RILQALRRYVRAENKDRLHTIRHYQHVLAVDPE
KAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVA
QEIQEEIDELLQEQRADMDQFTASISETPVDVR
VSSEESEEIPPFHPFHPFPALPENEGSGVGEQD
GGLIGAEEKVINSKNKVDENMVIDETLDVKEMIF
NAERVGGLEEERESVGPLREDFSLSSSALIGLL
VIAVAIATVIVISLVMLRKRQYGTISHGIVEVD
PMLTPEERHLNKMQNHGYENPTYKYLEQMQI*VA
GSAAALAEGCRWAGRSHDSDRLPSSSRCQGLRL
TS*PPGL*DYIKYYCRTAISILLNG*KMVI*QY
MIYKP*MKKMIYCRYLM*FSFLN*SETPLPLYCL
THALNI**MGNVDFQ**TYMQAVVPVMLCKSTL
QPHSLSWLLFKEKKGSIPFLNELSGSC*EMGWN
REL*WPLKHVRDPRKMM*KDQFLEVQCFHGWIPV
CLHKSPVTKDVKGILFQRLLESLVYTDEGVRCC
FPSESSASTEISL*LIFDILHCLLFTYRSFLPF
TSPSNYVQMCRLLLSGLSPKALTLGLYFVPSPPP
PPVTGLVTFTQRRPHQGGGRWPRREHGEVCLCE
RKVVQAVPETESVDTVESFEQLCFRHRSLCNAC
TVDVDAHCFCFFFLFILNLKVLVTCGVFLFSCDC
FCFVFFLFPPL*PYSCLYPLCTD*TSPTNSLI*
SVENVHSLNTSINTLTSTET

Sequence APLP2.eAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGG
AAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAA
ATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGT
GGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACG
GTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGT
GCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGT
TTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTG
GAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAA
TTACTCATGATGTCAAAGTTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCA
GAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAA
GCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGG
CCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTCCA
GGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAG
ATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAAATTC
AAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGA
GGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGACACTCAGCCGGAGTTGTACCACCCAATGAAAAAA
GGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGA
CTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAG
TAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATC
AGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACC
TGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCACTG
ACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCTGGC
CCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACAATT
GTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTTCTG
GTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTATGCA
CAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
ALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTG
KWEPDPTGTKSCFETKEEVLQYCQEMYPELQIT
NVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLV
GEFVSDVLLVPEKCQFFHKERMEVCENHQHWHT
VVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYV
CCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDV
YKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVV
EDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKE
ITHDVKVPPTPLPTNDVDVYFETSADDNEHARFQ
KAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPK
AERQTLIQHFQAMVKALEKEAASEKQQLVETHL
ARVEAMLNDRRRMALENYLAALQSDPPRPHRILQ
ALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQ
MKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEI
QEEIDELLQEQRADMDQFTASISETPVDVRVSSE
ESEEIPPFHPFHPFPALPENEDTQPELYHPMKK
GSGVGEQDGGLIGAEEKVINSKNKVDENMVIDE
TLDVKEMIFNAERVGGLEEERESVGPLREDFSLS
SSALIGLLVIAVAIATVIVISLVMLRKRQYGTI
SHGIVEVDPMLTPEERHLNKMQNHGYENPTYKY
LEQMQI*VAGSAAALAEGCRCAGSSSLDFLQRH*
PSASTLSPHLHPLLSPAL*HSLREDHTKEAAAG
PGENTGRFVCVKGK*SRLSLKLSLWTLWKALNN
CVFVTGVFVMLVQLMSMLTASAFSFFLF*I*RFW
*PVVYFYFPVTVFVLFFSFFLPFDPIHVSTHYA
QIKLHLQTP*YDLWRMYTV*THQ*IL*LPPR

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WLLQPMPEQDLLLLSLKSQCFVGS*ICM*TFRLG
NGNLIQQAPRAALKQKKKFFSTVRRCIQSYRSQ
M*WRQTSGLVLTTGAGGTKSNARVALLHLSSVS
WVNL*VMSC*FQKSASFSTKSGWRCVRITSTGTR
*SKRHV*LRE*PYIATACCSHVG*TSSMALNMC
AALRQRLLDLCQKKRKRKMKRKRKRKMKRKTMM
FIKVNFLLKQIWKTSQKQLWMRMMRMRKKGRKWW
RTEITTMTPSKEMTTMRRILLNPAATAPCQTRK
LLMMSKFLQLLCQPMMLMCISRPLQMIMSMLAS
RRLRSSWRFGTATEWTG*RRNGKRQSFKLRTSPK
QRGRL*FSTSKPWLKL*RRKQPVRSSSWWRPTW
PEWKLC*MTAVGWLWRTTWLPCSLTRHGLIAFS
RPYGVMSVLRTKIAYIPSVITSMCWLLTQKRRPR
*NPR**HIST*LKKGGTKASLCSTKYLM*PKKF
KRKLMSSFRSSVQIWTSSLPQSQRPLWTSG*AL
RRVRRSHRSTPSTPSQPYLRTKTLSRSCTTQ*KK
DLEWESRMGD*SVPKRK*LTVRIKWMKTWSLTR
LWMLRK*FSMPRELEASRKSGNPWAHCGRTSV*
VAVLSLACWSSQWPLPRSSSSAW*C*GRGSMAPS
ATGSWRLIQCSPQKSVT*TRCRTMAMRTPPTNT
WSRCRFRWQGARQPWRRDADVQAPPLWTFSKGT
DPRPLLCPLTSTPSCHRPCDIHSEKTTPRRRPLA
QERTRGGLFV*KESSPGCP*N*VCGHCGKL*TI
VFSSQESL*CLYS*CRCSLLLLFLSFYFKSEGS
GNLWCIFIFL*LFLFCFFPFSSPLTLFMSLPTMH
RLNFTYKLLNMICGECTQFKHINKYFNFHRD

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GSCSQCRNRICCC*ASNRNVLWEVKYACEHSDW
EMGT*SNRHQELL*NKRRSSSVLSGDVSRATDHK
CDGGKPAG*Y*QLVPEGQKAMQESLCYTFQVSR
G*ICK*CPASSRKVPVFPQRADGGV*ESPALAH
GSQRGMSDSGNDLI*LRHAAPMWGRPVPWH*ICV
LPSDKDYWICVKRRGRGR*RGRGRGR*RGRL*C
L*K*ISY*SRSGRLHRSSCG*G**G*GRRGGSG
GGPRLLL*HLQRR*LQ*GESY*TRQRRHHVRQGN
YS*CQSSSNSSANQ*C*CVFRDLCR***ACSLP
EG*GAAGDSAPQPNGQGKEGMGRGRASS*EPPQ
SREADSDSALPSHG*SFREGSSQ*EAAAGGDPPG
PSGSYAE*PPSDGSGELPGCLAV*PATASSHSP
GLTALCPC*EQRSLTYHPSLPACVGC*PRKGGP
DEIPGDDTSPRD*RKEEPKPLSALQSTLCSPRNS
RGN**APSGAACRYGPVHCLNLRDPCGRPGEL*
GE*GDPTVPPLPPLPSPT*ERRHSAGVVPPNEK
RIWSGRAGWGTDRCRRESD*Q*E*SG*KHGH*RD
SGC*GNDFQCRESWRPRGRAGIRGPTAGGLQSE
*QCSHWPAGHRSGHCHGHRHQPGDAEEEAVWHH
QPRDRGG*SNAHPRRASPEQDAEPWL*EPHLQIP
GADADLGGRERGSPGGGMQMCRLLLSGLSPKAL
TLGLYFVPSPPPPPVTGLVTFTQRRPHQGGGRW
PRREHGEVCLCERKVVQAVPETESVDTVESFEQL
CFRHRSLCNACTVDVDAHCFCFFFLFILNLKVL
VTCGVFLFSCDCFCFVFFLFPPL*PYSCLYPLC
TD*TSPTNSLI*SVENVHSLNTSINTLTSTET

Sequence APLP2.dAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAG
TTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAA
AAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCAC
AAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGC
TGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGA
TGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGA
ATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGTTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGT
GTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAG
AAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGA
AGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCT
GGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGT
CATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAA
GCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCAC
TGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCT
GAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCA
TTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTT
CAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTAT
GGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCT
ACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATCGACT
GCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCATTCTT
TTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTTTTAA
ATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGGC
TGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAATGAG
CTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAGTTTC
TTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAGAGAG
CTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCACTGT
CTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCACTGA
CCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCC
CAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACAATTG
TGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTTCTGG
TAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTATGCAC
AGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEE
VLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFH
KERMEVCENHQHWHTVVKEACLTQGMTLYSYGM
LLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEED
EEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAA
VDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEE
NPTEPGSDGTMSDKEITHDVKVPPTPLPTNDVDV
YFETSADDNEHARFQKAKEQLEIRHRNRMDRVK
KEWEEAELQAKNLPKAERQTLIQHFQAMVKALE
KEAASEKQQLVETHLARVEAMLNDRRRMALENYL
AALQSDPPRPHRILQALRRYVRAENKDRLHTIR
HYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQ
SLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFT
ASISETPVDVRVSSEESEEIPPFHPFHPFPALP
ENEGSGVGEQDGGLIGAEEKVINSKNKVDENMV
IDETLDVKEMIFNAERVGGLEEERESVGPLREDF
SLSSSALIGLLVIAVAIATVIVISLVMLRKRQY
GTISHGIVEVDPMLTPEERHLNKMQNHGYENPT
YKYLEQMQI*VAGSAAALAEGCRWAGRSHDSDRL
PSSSRCQGLRLTS*PPGL*DYIKYYCRTAISIL
LNG*KMVI*QYMIYKP*MKKMIYCRYLM*FSFL
N*SETPLPLYCLTHALNI**MGNVDFQ**TYMQA
VVPVMLCKSTLQPHSLSWLLFKEKKGSIPFLNE
LSGSC*EMGWNREL*WPLKHVRDPRKMM*KDQF
LEVQCFHGWIPVCLHKSPVTKDVKGILFQRLLES
LVYTDEGVRCCFPSESSASTEISL*LIFDILHC
LLFTYRSFLPFTSPSNYVQMCRLLLSGLSPKAL
TLGLYFVPSPPPPPVTGLVTFTQRRPHQGGGRWP
RREHGEVCLCERKVVQAVPETESVDTVESFEQL
CFRHRSLCNACTVDVDAHCFCFFFLFILNLKVL
VTCGVFLFSCDCFCFVFFLFPPL*PYSCLYPLCT
D*TSPTNSLI*SVENVHSLNTSINTLTSTET

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICM*TFRLGNGNLIQQAPRAALKQKKK
FFSTVRRCIQSYRSQM*WRQTSGLVLTTGAGGT
KSNARVALLHLSSVSWVNL*VMSC*FQKSASFST
KSGWRCVRITSTGTR*SKRHV*LRE*PYIATAC
CSHVG*TSSMALNMCAALRQRLLDLCQKKRKRK
MKRKRKRKMKRKTMMFIKVNFLLKQIWKTSQKQL
WMRMMRMRKKGRKWWRTEITTMTPSKEMTTMRR
ILLNPAATAPCQTRKLLMMSKFLQLLCQPMMLM
CISRPLQMIMSMLASRRLRSSWRFGTATEWTG*R
RNGKRQSFKLRTSPKQRGRL*FSTSKPWLKL*R
RKQPVRSSSWWRPTWPEWKLC*MTAVGWLWRTT
WLPCSLTRHGLIAFSRPYGVMSVLRTKIAYIPSV
ITSMCWLLTQKRRPR*NPR**HIST*LKKGGTK
ASLCSTKYLM*PKKFKRKLMSSFRSSVQIWTSS
LPQSQRPLWTSG*ALRRVRRSHRSTPSTPSQPYL
RTKDLEWESRMGD*SVPKRK*LTVRIKWMKTWS
LTRLWMLRK*FSMPRELEASRKSGNPWAHCGRT
SV*VAVLSLACWSSQWPLPRSSSSAW*C*GRGSM
APSATGSWRLIQCSPQKSVT*TRCRTMAMRTPP
TNTWSRCRFRWQGARQPWRRDAGGPEDPTIPID
CQAAAAARGCV*HPDLLDCRTI*STTVELQFPFF
*MGEKW*YNNI*YINLK*KK*SIADI*CSFLF*
INQKPHFHCIV*HMLSIYNKWEMSIFNNRLICR
LSFRLCCVSQLFSLIHCPGFYLKKKKAVFPF*MS
FQEVAEKWGGIGNCNGH*ST*ETLAK*CERTSF
LKSSVSTAGYLCVSIKVLSPRTLKAFYSSVF*R
A*CIQMRVSAAAFLRNPVLPQRLACSLYLTFFTV
CCLPTVAFYRSLPLPTMSRCAGSSSLDFLQRH*
PSASTLSPHLHPLLSPAL*HSLREDHTKEAAAG
PGENTGRFVCVKGK*SRLSLKLSLWTLWKALNNC
VFVTGVFVMLVQLMSMLTASAFSFFLF*I*RFW
*PVVYFYFPVTVFVLFFSFFLPFDPIHVSTHYA
QIKLHLQTP*YDLWRMYTV*THQ*IL*LPPR

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACEHSDWEMGT*SNRHQELL*NKRRS
SSVLSGDVSRATDHKCDGGKPAG*Y*QLVPEGQ
KAMQESLCYTFQVSRG*ICK*CPASSRKVPVFP
QRADGGV*ESPALAHGSQRGMSDSGNDLI*LRHA
APMWGRPVPWH*ICVLPSDKDYWICVKRRGRGR
*RGRGRGR*RGRL*CL*K*ISY*SRSGRLHRSS
CG*G**G*GRRGGSGGGPRLLL*HLQRR*LQ*GE
SY*TRQRRHHVRQGNYS*CQSSSNSSANQ*C*C
VFRDLCR***ACSLPEG*GAAGDSAPQPNGQGK
EGMGRGRASS*EPPQSREADSDSALPSHG*SFRE
GSSQ*EAAAGGDPPGPSGSYAE*PPSDGSGELP
GCLAV*PATASSHSPGLTALCPC*EQRSLTYHP
SLPACVGC*PRKGGPDEIPGDDTSPRD*RKEEPK
PLSALQSTLCSPRNSRGN**APSGAACRYGPVH
CLNLRDPCGRPGEL*GE*GDPTVPPLPPLPSPT
*ERRIWSGRAGWGTDRCRRESD*Q*E*SG*KHGH
*RDSGC*GNDFQCRESWRPRGRAGIRGPTAGGL
QSE*QCSHWPAGHRSGHCHGHRHQPGDAEEEAV
WHHQPRDRGG*SNAHPRRASPEQDAEPWL*EPHL
QIPGADADLGGRERGSPGGGMQVGRKIPRFRST
AKQQPLPGAASDILTSWTVGLYKVLL*NCNFHS
FKWVKNGNITIYDI*TLNEKNDLLQIFDVVFFFK
LIRNPTSIVLSDTCSQYIINGKCRFSIIDLYAG
CRSGYVV*VNSSASFTVLAFI*RKKRQYSLFK*
AFRKLLRNGVE*GTVMATEARERPSQNDVKGPVS
*SPVFPRLDTCVSP*KSCHQGR*RHFIPASSRE
LSVYR*GCPLLLSFGIQCFHRD*PVAYI*HSSL
SVVYLP*LFTVHFPFQLCPDVQAPPLWTFSKGTD
PRPLLCPLTSTPSCHRPCDIHSEKTTPRRRPLA
QERTRGGLFV*KESSPGCP*N*VCGHCGKL*TI
VFSSQESL*CLYS*CRCSLLLLFLSFYFKSEGSG
NLWCIFIFL*LFLFCFFPFSSPLTLFMSLPTMH
RLNFTYKLLNMICGECTQFKHINKYFNFHRD

Sequence APLP2.cAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAG
TTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAA
AAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCAC
AAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGC
TGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGA
TGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGA
ATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCG
GGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAG
GATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGC
ATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAA
GAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTG
GAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTC
ATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGA
AAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTA
GCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCC
GGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGA
TGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATT
TTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGC
TGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGA
TCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTG
GCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTC
TGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATA
TATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGT
CTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTC
TTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAA
TAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCT
GTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCT
GCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTT
CACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCA
CCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTG
AAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTG
TACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGAC
TGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATAT
GATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEE
VLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFH
KERMEVCENHQHWHTVVKEACLTQGMTLYSYGM
LLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEED
EEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAA
VDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEE
NPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCR
AVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESE
DYCMAVCKAMIPPTPLPTNDVDVYFETSADDNE
HARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAK
NLPKAERQTLIQHFQAMVKALEKEAASEKQQLV
ETHLARVEAMLNDRRRMALENYLAALQSDPPRP
HRILQALRRYVRAENKDRLHTIRHYQHVLAVDPE
KAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYV
AQEIQEEIDELLQEQRADMDQFTASISETPVDV
RVSSEESEEIPPFHPFHPFPALPENEGSGVGEQD
GGLIGAEEKVINSKNKVDENMVIDETLDVKEMI
FNAERVGGLEEERESVGPLREDFSLSSSALIGL
LVIAVAIATVIVISLVMLRKRQYGTISHGIVEVD
PMLTPEERHLNKMQNHGYENPTYKYLEQMQI*V
AGSAAALAEGCRWAGRSHDSDRLPSSSRCQGLR
LTS*PPGL*DYIKYYCRTAISILLNG*KMVI*QY
MIYKP*MKKMIYCRYLM*FSFLN*SETPLPLYC
LTHALNI**MGNVDFQ**TYMQAVVPVMLCKST
LQPHSLSWLLFKEKKGSIPFLNELSGSC*EMGWN
REL*WPLKHVRDPRKMM*KDQFLEVQCFHGWIP
VCLHKSPVTKDVKGILFQRLLESLVYTDEGVRC
CFPSESSASTEISL*LIFDILHCLLFTYRSFLPF
TSPSNYVQMCRLLLSGLSPKALTLGLYFVPSPP
PPPVTGLVTFTQRRPHQGGGRWPRREHGEVCLC
ERKVVQAVPETESVDTVESFEQLCFRHRSLCNAC
TVDVDAHCFCFFFLFILNLKVLVTCGVFLFSCD
CFCFVFFLFPPL*PYSCLYPLCTD*TSPTNSLI
*SVENVHSLNTSINTLTSTET

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICM*TFRLGNGNLIQQAPRAALKQKKK
FFSTVRRCIQSYRSQM*WRQTSGLVLTTGAGGT
KSNARVALLHLSSVSWVNL*VMSC*FQKSASFST
KSGWRCVRITSTGTR*SKRHV*LRE*PYIATAC
CSHVG*TSSMALNMCAALRQRLLDLCQKKRKRK
MKRKRKRKMKRKTMMFIKVNFLLKQIWKTSQKQL
WMRMMRMRKKGRKWWRTEITTMTPSKEMTTMRR
ILLNPAATAPCQTRKLLMMSKLSAPRRR*RGPA
GP*CLVGTSTSPRESACALYMVAAAATGTILSLR
IIVWLCVKR*FLQLLCQPMMLMCISRPLQMIMS
MLASRRLRSSWRFGTATEWTG*RRNGKRQSFKL
RTSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWW
RPTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGL
IAFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQ
KRRPR*NPR**HIST*LKKGGTKASLCSTKYLM*
PKKFKRKLMSSFRSSVQIWTSSLPQSQRPLWTS
G*ALRRVRRSHRSTPSTPSQPYLRTKDLEWESR
MGD*SVPKRK*LTVRIKWMKTWSLTRLWMLRK*F
SMPRELEASRKSGNPWAHCGRTSV*VAVLSLAC
WSSQWPLPRSSSSAW*C*GRGSMAPSATGSWRL
IQCSPQKSVT*TRCRTMAMRTPPTNTWSRCRFRW
QGARQPWRRDAGGPEDPTIPIDCQAAAAARGCV
*HPDLLDCRTI*STTVELQFPFF*MGEKW*YNN
I*YINLK*KK*SIADI*CSFLF*INQKPHFHCIV
*HMLSIYNKWEMSIFNNRLICRLSFRLCCVSQL
FSLIHCPGFYLKKKKAVFPF*MSFQEVAEKWGG
IGNCNGH*ST*ETLAK*CERTSFLKSSVSTAGYL
CVSIKVLSPRTLKAFYSSVF*RA*CIQMRVSAA
AFLRNPVLPQRLACSLYLTFFTVCCLPTVAFYR
SLPLPTMSRCAGSSSLDFLQRH*PSASTLSPHLH
PLLSPAL*HSLREDHTKEAAAGPGENTGRFVCV
KGK*SRLSLKLSLWTLWKALNNCVFVTGVFVML
VQLMSMLTASAFSFFLF*I*RFW*PVVYFYFPVT
VFVLFFSFFLPFDPIHVSTHYAQIKLHLQTP*Y
DLWRMYTV*THQ*IL*LPPR

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACEHSDWEMGT*SNRHQELL*NKRRS
SSVLSGDVSRATDHKCDGGKPAG*Y*QLVPEGQ
KAMQESLCYTFQVSRG*ICK*CPASSRKVPVFP
QRADGGV*ESPALAHGSQRGMSDSGNDLI*LRHA
APMWGRPVPWH*ICVLPSDKDYWICVKRRGRGR
*RGRGRGR*RGRL*CL*K*ISY*SRSGRLHRSS
CG*G**G*GRRGGSGGGPRLLL*HLQRR*LQ*GE
SY*TRQRRHHVRQGNYS*CQSCLLPGGDDGALP
GRDASLVLRPLQGKVRALYIWWLRRQQEQF*V*
GLLYGCV*SDDSSNSSANQ*C*CVFRDLCR***A
CSLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*
EPPQSREADSDSALPSHG*SFREGSSQ*EAAAG
GDPPGPSGSYAE*PPSDGSGELPGCLAV*PATAS
SHSPGLTALCPC*EQRSLTYHPSLPACVGC*PR
KGGPDEIPGDDTSPRD*RKEEPKPLSALQSTLC
SPRNSRGN**APSGAACRYGPVHCLNLRDPCGRP
GEL*GE*GDPTVPPLPPLPSPT*ERRIWSGRAG
WGTDRCRRESD*Q*E*SG*KHGH*RDSGC*GND
FQCRESWRPRGRAGIRGPTAGGLQSE*QCSHWPA
GHRSGHCHGHRHQPGDAEEEAVWHHQPRDRGG*
SNAHPRRASPEQDAEPWL*EPHLQIPGADADLG
GRERGSPGGGMQVGRKIPRFRSTAKQQPLPGAAS
DILTSWTVGLYKVLL*NCNFHSFKWVKNGNITI
YDI*TLNEKNDLLQIFDVVFFFKLIRNPTSIVL
SDTCSQYIINGKCRFSIIDLYAGCRSGYVV*VNS
SASFTVLAFI*RKKRQYSLFK*AFRKLLRNGVE
*GTVMATEARERPSQNDVKGPVS*SPVFPRLDT
CVSP*KSCHQGR*RHFIPASSRELSVYR*GCPLL
LSFGIQCFHRD*PVAYI*HSSLSVVYLP*LFTV
HFPFQLCPDVQAPPLWTFSKGTDPRPLLCPLTS
TPSCHRPCDIHSEKTTPRRRPLAQERTRGGLFV*
KESSPGCP*N*VCGHCGKL*TIVFSSQESL*CL
YS*CRCSLLLLFLSFYFKSEGSGNLWCIFIFL*
LFLFCFFPFSSPLTLFMSLPTMHRLNFTYKLLNM
ICGECTQFKHINKYFNFHRD

Sequence APLP2.bAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAG
TTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAA
AAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCAC
AAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGC
TGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGA
TGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGA
ATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCG
GGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAG
GATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGC
ATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAA
GAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTG
GAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTC
ATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGA
AAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTA
GCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCC
GGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGACACTCAGCCGGAGTTGTACCA
CCCAATGAAAAAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATG
GTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGG
ACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCA
GTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCC
ACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATC
GACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCAT
TCTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTT
TTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGC
AGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAA
TGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAG
TTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAG
AGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCA
CTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCA
CTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCT
GGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACA
ATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTT
CTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTAT
GCACAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEE
VLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFH
KERMEVCENHQHWHTVVKEACLTQGMTLYSYGM
LLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEED
EEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAA
VDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEE
NPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCR
AVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESE
DYCMAVCKAMIPPTPLPTNDVDVYFETSADDNE
HARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAK
NLPKAERQTLIQHFQAMVKALEKEAASEKQQLV
ETHLARVEAMLNDRRRMALENYLAALQSDPPRP
HRILQALRRYVRAENKDRLHTIRHYQHVLAVDPE
KAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYV
AQEIQEEIDELLQEQRADMDQFTASISETPVDV
RVSSEESEEIPPFHPFHPFPALPENEDTQPELYH
PMKKGSGVGEQDGGLIGAEEKVINSKNKVDENM
VIDETLDVKEMIFNAERVGGLEEERESVGPLRE
DFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQ
YGTISHGIVEVDPMLTPEERHLNKMQNHGYENP
TYKYLEQMQI*VAGSAAALAEGCRWAGRSHDSD
RLPSSSRCQGLRLTS*PPGL*DYIKYYCRTAISI
LLNG*KMVI*QYMIYKP*MKKMIYCRYLM*FSF
LN*SETPLPLYCLTHALNI**MGNVDFQ**TYM
QAVVPVMLCKSTLQPHSLSWLLFKEKKGSIPFLN
ELSGSC*EMGWNREL*WPLKHVRDPRKMM*KDQ
FLEVQCFHGWIPVCLHKSPVTKDVKGILFQRLL
ESLVYTDEGVRCCFPSESSASTEISL*LIFDILH
CLLFTYRSFLPFTSPSNYVQMCRLLLSGLSPKA
LTLGLYFVPSPPPPPVTGLVTFTQRRPHQGGGR
WPRREHGEVCLCERKVVQAVPETESVDTVESFEQ
LCFRHRSLCNACTVDVDAHCFCFFFLFILNLKV
LVTCGVFLFSCDCFCFVFFLFPPL*PYSCLYPL
CTD*TSPTNSLI*SVENVHSLNTSINTLTSTET

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICM*TFRLGNGNLIQQAPRAALKQKKK
FFSTVRRCIQSYRSQM*WRQTSGLVLTTGAGGT
KSNARVALLHLSSVSWVNL*VMSC*FQKSASFST
KSGWRCVRITSTGTR*SKRHV*LRE*PYIATAC
CSHVG*TSSMALNMCAALRQRLLDLCQKKRKRK
MKRKRKRKMKRKTMMFIKVNFLLKQIWKTSQKQL
WMRMMRMRKKGRKWWRTEITTMTPSKEMTTMRR
ILLNPAATAPCQTRKLLMMSKLSAPRRR*RGPA
GP*CLVGTSTSPRESACALYMVAAAATGTILSLR
IIVWLCVKR*FLQLLCQPMMLMCISRPLQMIMS
MLASRRLRSSWRFGTATEWTG*RRNGKRQSFKL
RTSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWW
RPTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGL
IAFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQ
KRRPR*NPR**HIST*LKKGGTKASLCSTKYLM*
PKKFKRKLMSSFRSSVQIWTSSLPQSQRPLWTS
G*ALRRVRRSHRSTPSTPSQPYLRTKTLSRSCT
TQ*KKDLEWESRMGD*SVPKRK*LTVRIKWMKTW
SLTRLWMLRK*FSMPRELEASRKSGNPWAHCGR
TSV*VAVLSLACWSSQWPLPRSSSSAW*C*GRG
SMAPSATGSWRLIQCSPQKSVT*TRCRTMAMRTP
PTNTWSRCRFRWQGARQPWRRDAGGPEDPTIPI
DCQAAAAARGCV*HPDLLDCRTI*STTVELQFP
FF*MGEKW*YNNI*YINLK*KK*SIADI*CSFLF
*INQKPHFHCIV*HMLSIYNKWEMSIFNNRLIC
RLSFRLCCVSQLFSLIHCPGFYLKKKKAVFPF*
MSFQEVAEKWGGIGNCNGH*ST*ETLAK*CERTS
FLKSSVSTAGYLCVSIKVLSPRTLKAFYSSVF*
RA*CIQMRVSAAAFLRNPVLPQRLACSLYLTFF
TVCCLPTVAFYRSLPLPTMSRCAGSSSLDFLQRH
*PSASTLSPHLHPLLSPAL*HSLREDHTKEAAA
GPGENTGRFVCVKGK*SRLSLKLSLWTLWKALN
NCVFVTGVFVMLVQLMSMLTASAFSFFLF*I*RF
W*PVVYFYFPVTVFVLFFSFFLPFDPIHVSTHY
AQIKLHLQTP*YDLWRMYTV*THQ*IL*LPPR

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACEHSDWEMGT*SNRHQELL*NKRRS
SSVLSGDVSRATDHKCDGGKPAG*Y*QLVPEGQ
KAMQESLCYTFQVSRG*ICK*CPASSRKVPVFP
QRADGGV*ESPALAHGSQRGMSDSGNDLI*LRHA
APMWGRPVPWH*ICVLPSDKDYWICVKRRGRGR
*RGRGRGR*RGRL*CL*K*ISY*SRSGRLHRSS
CG*G**G*GRRGGSGGGPRLLL*HLQRR*LQ*GE
SY*TRQRRHHVRQGNYS*CQSCLLPGGDDGALP
GRDASLVLRPLQGKVRALYIWWLRRQQEQF*V*
GLLYGCV*SDDSSNSSANQ*C*CVFRDLCR***A
CSLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*
EPPQSREADSDSALPSHG*SFREGSSQ*EAAAG
GDPPGPSGSYAE*PPSDGSGELPGCLAV*PATAS
SHSPGLTALCPC*EQRSLTYHPSLPACVGC*PR
KGGPDEIPGDDTSPRD*RKEEPKPLSALQSTLC
SPRNSRGN**APSGAACRYGPVHCLNLRDPCGRP
GEL*GE*GDPTVPPLPPLPSPT*ERRHSAGVVP
PNEKRIWSGRAGWGTDRCRRESD*Q*E*SG*KH
GH*RDSGC*GNDFQCRESWRPRGRAGIRGPTAGG
LQSE*QCSHWPAGHRSGHCHGHRHQPGDAEEEA
VWHHQPRDRGG*SNAHPRRASPEQDAEPWL*EP
HLQIPGADADLGGRERGSPGGGMQVGRKIPRFRS
TAKQQPLPGAASDILTSWTVGLYKVLL*NCNFH
SFKWVKNGNITIYDI*TLNEKNDLLQIFDVVFF
FKLIRNPTSIVLSDTCSQYIINGKCRFSIIDLYA
GCRSGYVV*VNSSASFTVLAFI*RKKRQYSLFK
*AFRKLLRNGVE*GTVMATEARERPSQNDVKGP
VS*SPVFPRLDTCVSP*KSCHQGR*RHFIPASSR
ELSVYR*GCPLLLSFGIQCFHRD*PVAYI*HSS
LSVVYLP*LFTVHFPFQLCPDVQAPPLWTFSKG
TDPRPLLCPLTSTPSCHRPCDIHSEKTTPRRRPL
AQERTRGGLFV*KESSPGCP*N*VCGHCGKL*T
IVFSSQESL*CLYS*CRCSLLLLFLSFYFKSEG
SGNLWCIFIFL*LFLFCFFPFSSPLTLFMSLPTM
HRLNFTYKLLNMICGECTQFKHINKYFNFHRD

Sequence APLP2.aAug05

CGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGTGGGGACCGGGCGAACGCCGGAGAGTCGTCTCCTTCGCCCGCCGGAGGAGCGCGGACTGCGGGC
GGGCAGCGGGGTCCGCGGCAGGGCGGGCCGGCGGTGCGGCGCCGGGGGTCCGGCTCTGCGGCGGGTCTGGCGCGCCCTCCCCCGCCCGTCCCTGCTTGGC
GCAGTGCTTAGCGCTGGAGGTCGGGCTGCCTCAGCCGCCGCAGAGGCCAAATGAAAGGCGTCGGGGCTGCTGGGGGCGGCCGTCCGCCTCGGCCGACCCA
GCCCCCAACCCGCCCGCGGCGCCGCCACCTCCTCCGGGGACCCAGTGCCCCCGGGCGCCCGCCCCGCCCTCCCCCGGGACAATGCCAGGGCGCTCCTCTC
CCGCCGGAGTCCGCGGCTGGGCTTTCTCCAGGGGCGGCCCCGCGCGGACCCCGTACGCTCCCTCGCGCGGCACCGGGGCCTCGGCTCCGGGCCTCCCACC
TGCGAGCGGCGGGCTTCGCCTTTGTTGCCAGGTGGACGCGGCCCCGGCCTTCGCGCGCGGCAGGGATGCTGCGAGCCCCGGGGGAGCTCCCGCGCCAGGC
CGCCCGCTGCTCCCTCTGCCGCCTGGGGCCGGGTCGCGGACGCGCATTTTTTAAGTGGCGCTGTTTGCCTGCGTCCGTAGACCGAGGAAACCCGCTCTGG
GCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGA
AATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAA
TGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTG
GGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGG
TAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTG
CTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTT
TATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGG
AGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAAT
TACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCGGGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGC
GTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTC
TGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCG
CAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAA
GCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTC
GGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAA
AGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTG
ATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGC
GTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTT
CCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAAT
AAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCG
TGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGT
GATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAAC
CATGGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAA
GATCCCACGATTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTG
TAGAACTGCAATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATAT
TTGATGTAGTTTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTC
AATAATAGACTTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGG
CAGTATTCCCTTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAA
ATGATGTGAAAGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTT
TATTCCAGCGTCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCT
TATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCT
GGACTTTCTCCAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCAC
ACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACAC
TGTGGAAAGCTTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTT
ATTTTAAATCTGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATT
CATGTCTCTACCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTT
AACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Single exon skipping

Frame 0

RRLPWRWPATSRWGPGERRRVVSFARRRSADCG
RAAGSAAGRAGGAAPGVRLCGGSGAPSPARPCLA
QCLALEVGLPQPPQRPNERRRGCWGRPSASADP
APNPPAAPPPPPGTQCPRAPAPPSPGTMPGRSS
PAGVRGWAFSRGGPARTPYAPSRGTGASAPGLPP
ASGGLRLCCQVDAAPAFARGRDAASPGGAPAPG
RPLLPLPPGAGSRTRIF*VALFACVRRPRKPAL
GSCSQCRNRICCC*ASNRNVLWEVKYACEHSDWE
MGT*SNRHQELL*NKRRSSSVLSGDVSRATDHK
CDGGKPAG*Y*QLVPEGQKAMQESLCYTFQVSR
G*ICK*CPASSRKVPVFPQRADGGV*ESPALAHG
SQRGMSDSGNDLI*LRHAAPMWGRPVPWH*ICV
LPSDKDYWICVKRRGRGR*RGRGRGR*RGRL*C
L*K*ISY*SRSGRLHRSSCG*G**G*GRRGGSGG
GPRLLL*HLQRR*LQ*GESY*TRQRRHHVRQGN
YS*CQSCLLPGGDDGALPGRDASLVLRPLQGKV
RALYIWWLRRQQEQF*V*GLLYGCV*SDDSSNSS
ANQ*C*CVFRDLCR***ACSLPEG*GAAGDSAP
QPNGQGKEGMGRGRASS*EPPQSREADSDSALP
SHG*SFREGSSQ*EAAAGGDPPGPSGSYAE*PPS
DGSGELPGCLAV*PATASSHSPGLTALCPC*EQ
RSLTYHPSLPACVGC*PRKGGPDEIPGDDTSPR
D*RKEEPKPLSALQSTLCSPRNSRGN**APSGAA
CRYGPVHCLNLRDPCGRPGEL*GE*GDPTVPPL
PPLPSPT*ERRIWSGRAGWGTDRCRRESD*Q*E
*SG*KHGH*RDSGC*GNDFQCRESWRPRGRAGIR
GPTAGGLQSE*QCSHWPAGHRSGHCHGHRHQPG
DAEEEAVWHHQPRDRGG*SNAHPRRASPEQDAE
PWL*EPHLQIPGADADLGGRERGSPGGGMQVGRK
IPRFRSTAKQQPLPGAASDILTSWTVGLYKVLL
*NCNFHSFKWVKNGNITIYDI*TLNEKNDLLQI
FDVVFFFKLIRNPTSIVLSDTCSQYIINGKCRFS
IIDLYAGCRSGYVV*VNSSASFTVLAFI*RKKR
QYSLFK*AFRKLLRNGVE*GTVMATEARERPSQ
NDVKGPVS*SPVFPRLDTCVSP*KSCHQGR*RHF
IPASSRELSVYR*GCPLLLSFGIQCFHRD*PVA
YI*HSSLSVVYLP*LFTVHFPFQLCPDVQAPPL
WTFSKGTDPRPLLCPLTSTPSCHRPCDIHSEKTT
PRRRPLAQERTRGGLFV*KESSPGCP*N*VCGH
CGKL*TIVFSSQESL*CLYS*CRCSLLLLFLSF
YFKSEGSGNLWCIFIFL*LFLFCFFPFSSPLTLF
MSLPTMHRLNFTYKLLNMICGECTQFKHINKYF
NFHRD

Frame 1

GACLGAGRLHRGGDRANAGESSPSPAGGARTAG
GQRGPRQGGPAVRRRGSGSAAGLARPPPPVPAW
RSA*RWRSGCLSRRRGQMKGVGAAGGGRPPRPTQ
PPTRPRRRHLLRGPSAPGRPPRPPPGQCQGAPL
PPESAAGLSPGAAPRGPRTLPRAAPGPRLRASH
LRAAGFAFVARWTRPRPSRAAGMLRAPGELPRQA
ARCSLCRLGPGRGRAFFKWRCLPASVDRGNPLW
ALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTG
KWEPDPTGTKSCFETKEEVLQYCQEMYPELQITN
VMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLV
GEFVSDVLLVPEKCQFFHKERMEVCENHQHWHT
VVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVC
CPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDV
YKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVV
EDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEI
THDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKC
VRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTP
LPTNDVDVYFETSADDNEHARFQKAKEQLEIRHR
NRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQ
AMVKALEKEAASEKQQLVETHLARVEAMLNDRR
RMALENYLAALQSDPPRPHRILQALRRYVRAENK
DRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHV
IEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQ
RADMDQFTASISETPVDVRVSSEESEEIPPFHPF
HPFPALPENEGSGVGEQDGGLIGAEEKVINSKN
KVDENMVIDETLDVKEMIFNAERVGGLEEERES
VGPLREDFSLSSSALIGLLVIAVAIATVIVISLV
MLRKRQYGTISHGIVEVDPMLTPEERHLNKMQN
HGYENPTYKYLEQMQI*VAGSAAALAEGCRWAG
RSHDSDRLPSSSRCQGLRLTS*PPGL*DYIKYYC
RTAISILLNG*KMVI*QYMIYKP*MKKMIYCRY
LM*FSFLN*SETPLPLYCLTHALNI**MGNVDF
Q**TYMQAVVPVMLCKSTLQPHSLSWLLFKEKKG
SIPFLNELSGSC*EMGWNREL*WPLKHVRDPRK
MM*KDQFLEVQCFHGWIPVCLHKSPVTKDVKGI
LFQRLLESLVYTDEGVRCCFPSESSASTEISL*L
IFDILHCLLFTYRSFLPFTSPSNYVQMCRLLLS
GLSPKALTLGLYFVPSPPPPPVTGLVTFTQRRP
HQGGGRWPRREHGEVCLCERKVVQAVPETESVDT
VESFEQLCFRHRSLCNACTVDVDAHCFCFFFLF
ILNLKVLVTCGVFLFSCDCFCFVFFLFPPL*PY
SCLYPLCTD*TSPTNSLI*SVENVHSLNTSINTL
TSTET

Frame 2

APALALAGYIEVGTGRTPESRLLRPPEERGLRA
GSGVRGRAGRRCGAGGPALRRVWRALPRPSLLG
AVLSAGGRAASAAAEAK*KASGLLGAAVRLGRP
SPQPARGAATSSGDPVPPGARPALPRDNARALLS
RRSPRLGFLQGRPRADPVRSLARHRGLGSGPPT
CERRASPLLPGGRGPGLRARQGCCEPRGSSRAR
PPAAPSAAWGRVADAHFLSGAVCLRP*TEETRSG
LLQPMPEQDLLLLSLKSQCFVGS*ICM*TFRLG
NGNLIQQAPRAALKQKKKFFSTVRRCIQSYRSQ
M*WRQTSGLVLTTGAGGTKSNARVALLHLSSVSW
VNL*VMSC*FQKSASFSTKSGWRCVRITSTGTR
*SKRHV*LRE*PYIATACCSHVG*TSSMALNMC
AALRQRLLDLCQKKRKRKMKRKRKRKMKRKTMMF
IKVNFLLKQIWKTSQKQLWMRMMRMRKKGRKWW
RTEITTMTPSKEMTTMRRILLNPAATAPCQTRK
LLMMSKLSAPRRR*RGPAGP*CLVGTSTSPRESA
CALYMVAAAATGTILSLRIIVWLCVKR*FLQLL
CQPMMLMCISRPLQMIMSMLASRRLRSSWRFGT
ATEWTG*RRNGKRQSFKLRTSPKQRGRL*FSTSK
PWLKL*RRKQPVRSSSWWRPTWPEWKLC*MTAV
GWLWRTTWLPCSLTRHGLIAFSRPYGVMSVLRT
KIAYIPSVITSMCWLLTQKRRPR*NPR**HIST*
LKKGGTKASLCSTKYLM*PKKFKRKLMSSFRSS
VQIWTSSLPQSQRPLWTSG*ALRRVRRSHRSTP
STPSQPYLRTKDLEWESRMGD*SVPKRK*LTVRI
KWMKTWSLTRLWMLRK*FSMPRELEASRKSGNP
WAHCGRTSV*VAVLSLACWSSQWPLPRSSSSAW
*C*GRGSMAPSATGSWRLIQCSPQKSVT*TRCRT
MAMRTPPTNTWSRCRFRWQGARQPWRRDAGGPE
DPTIPIDCQAAAAARGCV*HPDLLDCRTI*STT
VELQFPFF*MGEKW*YNNI*YINLK*KK*SIADI
*CSFLF*INQKPHFHCIV*HMLSIYNKWEMSIF
NNRLICRLSFRLCCVSQLFSLIHCPGFYLKKKK
AVFPF*MSFQEVAEKWGGIGNCNGH*ST*ETLAK
*CERTSFLKSSVSTAGYLCVSIKVLSPRTLKAF
YSSVF*RA*CIQMRVSAAAFLRNPVLPQRLACS
LYLTFFTVCCLPTVAFYRSLPLPTMSRCAGSSSL
DFLQRH*PSASTLSPHLHPLLSPAL*HSLREDH
TKEAAAGPGENTGRFVCVKGK*SRLSLKLSLWT
LWKALNNCVFVTGVFVMLVQLMSMLTASAFSFFL
F*I*RFW*PVVYFYFPVTVFVLFFSFFLPFDPI
HVSTHYAQIKLHLQTP*YDLWRMYTV*THQ*IL
*LPPR

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