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Sequences

Sequence APLP2.hAug05

GCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCT
TTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAGAATCACC
AGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGG
CACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAG
GAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATAATGAGCATG
CTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAA
CCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAG
ACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATC
GCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAA
GGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCC
CAAGAAATTCAAGAGGAAATTGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATG
AAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACT
GCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGG
AAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATG
AGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGA
TTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCA
ATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGT
TTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGAC
TTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCC
TTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAA
AGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCG
TCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGAC
ATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTC
CAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGG
CGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGC
TTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATC
TGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTA
CCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACC
GAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

ATDHKCDGGKPAG*Y*QLVPEGQKAMQESLCYT
FQVSRG*ICK*CPASSRKVPVFPQRADGGV*ESP
ALAHGSQRGMSDSGNDLI*LRHAAPMWGRPVPW
H*ICVLPSDKDYWICVKRRGRGR*RGRGRGR*R
GRL*CL*K*ISY*SRSGRLHRSSCG*G**G**AC
SLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*E
PPQSREADSDSALPSHG*SFREGSSQ*EAAAGG
DPPGPSGSYAE*PPSDGSGELPGCLAV*PATASS
HSPGLTALCPC*EQRSLTYHPSLPACVGC*PRK
GGPDEIPGDDTSPRD*RKEEPKPLSALQSTLCS
PRNSRGNWIWSGRAGWGTDRCRRESD*Q*E*SG*
KHGH*RDSGC*GNDFQCRESWRPRGRAGIRGPT
AGGLQSE*QCSHWPAGHRSGHCHGHRHQPGDAE
EEAVWHHQPRDRGG*SNAHPRRASPEQDAEPWL*
EPHLQIPGADADLGGRERGSPGGGMQVGRKIPR
FRSTAKQQPLPGAASDILTSWTVGLYKVLL*NC
NFHSFKWVKNGNITIYDI*TLNEKNDLLQIFDVV
FFFKLIRNPTSIVLSDTCSQYIINGKCRFSIID
LYAGCRSGYVV*VNSSASFTVLAFI*RKKRQYS
LFK*AFRKLLRNGVE*GTVMATEARERPSQNDVK
GPVS*SPVFPRLDTCVSP*KSCHQGR*RHFIPA
SSRELSVYR*GCPLLLSFGIQCFHRD*PVAYI*
HSSLSVVYLP*LFTVHFPFQLCPDVQAPPLWTFS
KGTDPRPLLCPLTSTPSCHRPCDIHSEKTTPRR
RPLAQERTRGGLFV*KESSPGCP*N*VCGHCGK
L*TIVFSSQESL*CLYS*CRCSLLLLFLSFYFKS
EGSGNLWCIFIFL*LFLFCFFPFSSPLTLFMSL
PTMHRLNFTYKLLNMICGECTQFKHINKYFNFH
RD

Frame 1

LQITNVMEANQRVSIDNWCRRDKKQCKSRFVTP
FKCLVGEFVSDVLLVPEKCQFFHKERMEVCENH
QHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHG
TEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEE
EDYDVYKSEFPTEADLEDFTEAAVDEDDEDNEH
ARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKN
LPKAERQTLIQHFQAMVKALEKEAASEKQQLVE
THLARVEAMLNDRRRMALENYLAALQSDPPRPH
RILQALRRYVRAENKDRLHTIRHYQHVLAVDPEK
AAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVA
QEIQEEIGSGVGEQDGGLIGAEEKVINSKNKVD
ENMVIDETLDVKEMIFNAERVGGLEEERESVGPL
REDFSLSSSALIGLLVIAVAIATVIVISLVMLR
KRQYGTISHGIVEVDPMLTPEERHLNKMQNHGY
ENPTYKYLEQMQI*VAGSAAALAEGCRWAGRSHD
SDRLPSSSRCQGLRLTS*PPGL*DYIKYYCRTA
ISILLNG*KMVI*QYMIYKP*MKKMIYCRYLM*
FSFLN*SETPLPLYCLTHALNI**MGNVDFQ**T
YMQAVVPVMLCKSTLQPHSLSWLLFKEKKGSIP
FLNELSGSC*EMGWNREL*WPLKHVRDPRKMM*
KDQFLEVQCFHGWIPVCLHKSPVTKDVKGILFQR
LLESLVYTDEGVRCCFPSESSASTEISL*LIFD
ILHCLLFTYRSFLPFTSPSNYVQMCRLLLSGLS
PKALTLGLYFVPSPPPPPVTGLVTFTQRRPHQGG
GRWPRREHGEVCLCERKVVQAVPETESVDTVES
FEQLCFRHRSLCNACTVDVDAHCFCFFFLFILN
LKVLVTCGVFLFSCDCFCFVFFLFPPL*PYSCLY
PLCTD*TSPTNSLI*SVENVHSLNTSINTLTST
ET

Frame 2

YRSQM*WRQTSGLVLTTGAGGTKSNARVALLHL
SSVSWVNL*VMSC*FQKSASFSTKSGWRCVRIT
STGTR*SKRHV*LRE*PYIATACCSHVG*TSSM
ALNMCAALRQRLLDLCQKKRKRKMKRKRKRKMKR
KTMMFIKVNFLLKQIWKTSQKQLWMRMMRIMSM
LASRRLRSSWRFGTATEWTG*RRNGKRQSFKLR
TSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWWR
PTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGLI
AFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQK
RRPR*NPR**HIST*LKKGGTKASLCSTKYLM*P
KKFKRKLDLEWESRMGD*SVPKRK*LTVRIKWM
KTWSLTRLWMLRK*FSMPRELEASRKSGNPWAH
CGRTSV*VAVLSLACWSSQWPLPRSSSSAW*C*G
RGSMAPSATGSWRLIQCSPQKSVT*TRCRTMAM
RTPPTNTWSRCRFRWQGARQPWRRDAGGPEDPT
IPIDCQAAAAARGCV*HPDLLDCRTI*STTVELQ
FPFF*MGEKW*YNNI*YINLK*KK*SIADI*CS
FLF*INQKPHFHCIV*HMLSIYNKWEMSIFNNR
LICRLSFRLCCVSQLFSLIHCPGFYLKKKKAVFP
F*MSFQEVAEKWGGIGNCNGH*ST*ETLAK*CE
RTSFLKSSVSTAGYLCVSIKVLSPRTLKAFYSS
VF*RA*CIQMRVSAAAFLRNPVLPQRLACSLYLT
FFTVCCLPTVAFYRSLPLPTMSRCAGSSSLDFL
QRH*PSASTLSPHLHPLLSPAL*HSLREDHTKE
AAAGPGENTGRFVCVKGK*SRLSLKLSLWTLWKA
LNNCVFVTGVFVMLVQLMSMLTASAFSFFLF*I
*RFW*PVVYFYFPVTVFVLFFSFFLPFDPIHVS
THYAQIKLHLQTP*YDLWRMYTV*THQ*IL*LPP
R

Sequence APLP2.cAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAG
TTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAA
AAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCAC
AAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGC
TGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGA
TGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGA
ATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCG
GGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAG
GATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGC
ATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAA
GAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTG
GAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTC
ATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGA
AAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTA
GCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCC
GGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGA
TGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATT
TTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGC
TGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGA
TCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTG
GCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTC
TGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATA
TATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGT
CTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTC
TTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAA
TAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCT
GTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCT
GCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTT
CACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCA
CCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTG
AAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTG
TACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGAC
TGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATAT
GATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEE
VLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFH
KERMEVCENHQHWHTVVKEACLTQGMTLYSYGM
LLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEED
EEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAA
VDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEE
NPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCR
AVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESE
DYCMAVCKAMIPPTPLPTNDVDVYFETSADDNE
HARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAK
NLPKAERQTLIQHFQAMVKALEKEAASEKQQLV
ETHLARVEAMLNDRRRMALENYLAALQSDPPRP
HRILQALRRYVRAENKDRLHTIRHYQHVLAVDPE
KAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYV
AQEIQEEIDELLQEQRADMDQFTASISETPVDV
RVSSEESEEIPPFHPFHPFPALPENEGSGVGEQD
GGLIGAEEKVINSKNKVDENMVIDETLDVKEMI
FNAERVGGLEEERESVGPLREDFSLSSSALIGL
LVIAVAIATVIVISLVMLRKRQYGTISHGIVEVD
PMLTPEERHLNKMQNHGYENPTYKYLEQMQI*V
AGSAAALAEGCRWAGRSHDSDRLPSSSRCQGLR
LTS*PPGL*DYIKYYCRTAISILLNG*KMVI*QY
MIYKP*MKKMIYCRYLM*FSFLN*SETPLPLYC
LTHALNI**MGNVDFQ**TYMQAVVPVMLCKST
LQPHSLSWLLFKEKKGSIPFLNELSGSC*EMGWN
REL*WPLKHVRDPRKMM*KDQFLEVQCFHGWIP
VCLHKSPVTKDVKGILFQRLLESLVYTDEGVRC
CFPSESSASTEISL*LIFDILHCLLFTYRSFLPF
TSPSNYVQMCRLLLSGLSPKALTLGLYFVPSPP
PPPVTGLVTFTQRRPHQGGGRWPRREHGEVCLC
ERKVVQAVPETESVDTVESFEQLCFRHRSLCNAC
TVDVDAHCFCFFFLFILNLKVLVTCGVFLFSCD
CFCFVFFLFPPL*PYSCLYPLCTD*TSPTNSLI
*SVENVHSLNTSINTLTSTET

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICM*TFRLGNGNLIQQAPRAALKQKKK
FFSTVRRCIQSYRSQM*WRQTSGLVLTTGAGGT
KSNARVALLHLSSVSWVNL*VMSC*FQKSASFST
KSGWRCVRITSTGTR*SKRHV*LRE*PYIATAC
CSHVG*TSSMALNMCAALRQRLLDLCQKKRKRK
MKRKRKRKMKRKTMMFIKVNFLLKQIWKTSQKQL
WMRMMRMRKKGRKWWRTEITTMTPSKEMTTMRR
ILLNPAATAPCQTRKLLMMSKLSAPRRR*RGPA
GP*CLVGTSTSPRESACALYMVAAAATGTILSLR
IIVWLCVKR*FLQLLCQPMMLMCISRPLQMIMS
MLASRRLRSSWRFGTATEWTG*RRNGKRQSFKL
RTSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWW
RPTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGL
IAFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQ
KRRPR*NPR**HIST*LKKGGTKASLCSTKYLM*
PKKFKRKLMSSFRSSVQIWTSSLPQSQRPLWTS
G*ALRRVRRSHRSTPSTPSQPYLRTKDLEWESR
MGD*SVPKRK*LTVRIKWMKTWSLTRLWMLRK*F
SMPRELEASRKSGNPWAHCGRTSV*VAVLSLAC
WSSQWPLPRSSSSAW*C*GRGSMAPSATGSWRL
IQCSPQKSVT*TRCRTMAMRTPPTNTWSRCRFRW
QGARQPWRRDAGGPEDPTIPIDCQAAAAARGCV
*HPDLLDCRTI*STTVELQFPFF*MGEKW*YNN
I*YINLK*KK*SIADI*CSFLF*INQKPHFHCIV
*HMLSIYNKWEMSIFNNRLICRLSFRLCCVSQL
FSLIHCPGFYLKKKKAVFPF*MSFQEVAEKWGG
IGNCNGH*ST*ETLAK*CERTSFLKSSVSTAGYL
CVSIKVLSPRTLKAFYSSVF*RA*CIQMRVSAA
AFLRNPVLPQRLACSLYLTFFTVCCLPTVAFYR
SLPLPTMSRCAGSSSLDFLQRH*PSASTLSPHLH
PLLSPAL*HSLREDHTKEAAAGPGENTGRFVCV
KGK*SRLSLKLSLWTLWKALNNCVFVTGVFVML
VQLMSMLTASAFSFFLF*I*RFW*PVVYFYFPVT
VFVLFFSFFLPFDPIHVSTHYAQIKLHLQTP*Y
DLWRMYTV*THQ*IL*LPPR

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACEHSDWEMGT*SNRHQELL*NKRRS
SSVLSGDVSRATDHKCDGGKPAG*Y*QLVPEGQ
KAMQESLCYTFQVSRG*ICK*CPASSRKVPVFP
QRADGGV*ESPALAHGSQRGMSDSGNDLI*LRHA
APMWGRPVPWH*ICVLPSDKDYWICVKRRGRGR
*RGRGRGR*RGRL*CL*K*ISY*SRSGRLHRSS
CG*G**G*GRRGGSGGGPRLLL*HLQRR*LQ*GE
SY*TRQRRHHVRQGNYS*CQSCLLPGGDDGALP
GRDASLVLRPLQGKVRALYIWWLRRQQEQF*V*
GLLYGCV*SDDSSNSSANQ*C*CVFRDLCR***A
CSLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*
EPPQSREADSDSALPSHG*SFREGSSQ*EAAAG
GDPPGPSGSYAE*PPSDGSGELPGCLAV*PATAS
SHSPGLTALCPC*EQRSLTYHPSLPACVGC*PR
KGGPDEIPGDDTSPRD*RKEEPKPLSALQSTLC
SPRNSRGN**APSGAACRYGPVHCLNLRDPCGRP
GEL*GE*GDPTVPPLPPLPSPT*ERRIWSGRAG
WGTDRCRRESD*Q*E*SG*KHGH*RDSGC*GND
FQCRESWRPRGRAGIRGPTAGGLQSE*QCSHWPA
GHRSGHCHGHRHQPGDAEEEAVWHHQPRDRGG*
SNAHPRRASPEQDAEPWL*EPHLQIPGADADLG
GRERGSPGGGMQVGRKIPRFRSTAKQQPLPGAAS
DILTSWTVGLYKVLL*NCNFHSFKWVKNGNITI
YDI*TLNEKNDLLQIFDVVFFFKLIRNPTSIVL
SDTCSQYIINGKCRFSIIDLYAGCRSGYVV*VNS
SASFTVLAFI*RKKRQYSLFK*AFRKLLRNGVE
*GTVMATEARERPSQNDVKGPVS*SPVFPRLDT
CVSP*KSCHQGR*RHFIPASSRELSVYR*GCPLL
LSFGIQCFHRD*PVAYI*HSSLSVVYLP*LFTV
HFPFQLCPDVQAPPLWTFSKGTDPRPLLCPLTS
TPSCHRPCDIHSEKTTPRRRPLAQERTRGGLFV*
KESSPGCP*N*VCGHCGKL*TIVFSSQESL*CL
YS*CRCSLLLLFLSFYFKSEGSGNLWCIFIFL*
LFLFCFFPFSSPLTLFMSLPTMHRLNFTYKLLNM
ICGECTQFKHINKYFNFHRD

Sequence APLP2.bAug05

AATATGAAGTCAGTTCCGGTTGGTGTAAAACCCCCGGGGCGGCGGCGAACTGGCTTTAGATGCTTCTGGGTCGCGGTGTGCTAAGCGAGGAGTCCGAGTG
TGTGAGCTTGAGAGCCGCGCGCTAGAGCGACCCGGCGAGGGATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCT
GGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCA
ATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAG
TTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAA
AAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCAC
AAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGC
TGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGA
TGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGA
ATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCG
GGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAG
GATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGC
ATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAA
GAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTG
GAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTC
ATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGA
AAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTA
GCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCC
GGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGACACTCAGCCGGAGTTGTACCA
CCCAATGAAAAAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATG
GTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGG
ACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCA
GTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCC
ACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAAGATCCCACGATTCCGATC
GACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTGTAGAACTGCAATTTCCAT
TCTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGTAGTTTTCTTTT
TTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGC
AGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGGCAGTATTCCCTTTTTAAA
TGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAAATGATGTGAAAGGACCAG
TTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGTCTTCTAG
AGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCTTATATTTGACATTCTTCA
CTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCTGGACTTTCTCCAAAGGCA
CTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCACACCAAGGAGGCGGCCGCT
GGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACACTGTGGAAAGCTTTGAACA
ATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTGAAGGTT
CTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATTCATGTCTCTACCCACTAT
GCACAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTTAACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

NMKSVPVGVKPPGRRRTGFRCFWVAVC*ARSPS
V*A*EPRARATRRGMAATGTAAAAATGRLLLLLL
VGLTAPALALAGYIEALAANAGTGFAVAEPQIA
MFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEE
VLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFH
KERMEVCENHQHWHTVVKEACLTQGMTLYSYGM
LLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEED
EEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAA
VDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEE
NPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCR
AVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESE
DYCMAVCKAMIPPTPLPTNDVDVYFETSADDNE
HARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAK
NLPKAERQTLIQHFQAMVKALEKEAASEKQQLV
ETHLARVEAMLNDRRRMALENYLAALQSDPPRP
HRILQALRRYVRAENKDRLHTIRHYQHVLAVDPE
KAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYV
AQEIQEEIDELLQEQRADMDQFTASISETPVDV
RVSSEESEEIPPFHPFHPFPALPENEDTQPELYH
PMKKGSGVGEQDGGLIGAEEKVINSKNKVDENM
VIDETLDVKEMIFNAERVGGLEEERESVGPLRE
DFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQ
YGTISHGIVEVDPMLTPEERHLNKMQNHGYENP
TYKYLEQMQI*VAGSAAALAEGCRWAGRSHDSD
RLPSSSRCQGLRLTS*PPGL*DYIKYYCRTAISI
LLNG*KMVI*QYMIYKP*MKKMIYCRYLM*FSF
LN*SETPLPLYCLTHALNI**MGNVDFQ**TYM
QAVVPVMLCKSTLQPHSLSWLLFKEKKGSIPFLN
ELSGSC*EMGWNREL*WPLKHVRDPRKMM*KDQ
FLEVQCFHGWIPVCLHKSPVTKDVKGILFQRLL
ESLVYTDEGVRCCFPSESSASTEISL*LIFDILH
CLLFTYRSFLPFTSPSNYVQMCRLLLSGLSPKA
LTLGLYFVPSPPPPPVTGLVTFTQRRPHQGGGR
WPRREHGEVCLCERKVVQAVPETESVDTVESFEQ
LCFRHRSLCNACTVDVDAHCFCFFFLFILNLKV
LVTCGVFLFSCDCFCFVFFLFPPL*PYSCLYPL
CTD*TSPTNSLI*SVENVHSLNTSINTLTSTET

Frame 1

I*SQFRLV*NPRGGGELALDASGSRCAKRGVRV
CELESRALERPGEGWRPPGPRPPQPRAGSCFCC
WWGSRRLPWRWPATSRLLQPMPEQDLLLLSLKSQ
CFVGS*ICM*TFRLGNGNLIQQAPRAALKQKKK
FFSTVRRCIQSYRSQM*WRQTSGLVLTTGAGGT
KSNARVALLHLSSVSWVNL*VMSC*FQKSASFST
KSGWRCVRITSTGTR*SKRHV*LRE*PYIATAC
CSHVG*TSSMALNMCAALRQRLLDLCQKKRKRK
MKRKRKRKMKRKTMMFIKVNFLLKQIWKTSQKQL
WMRMMRMRKKGRKWWRTEITTMTPSKEMTTMRR
ILLNPAATAPCQTRKLLMMSKLSAPRRR*RGPA
GP*CLVGTSTSPRESACALYMVAAAATGTILSLR
IIVWLCVKR*FLQLLCQPMMLMCISRPLQMIMS
MLASRRLRSSWRFGTATEWTG*RRNGKRQSFKL
RTSPKQRGRL*FSTSKPWLKL*RRKQPVRSSSWW
RPTWPEWKLC*MTAVGWLWRTTWLPCSLTRHGL
IAFSRPYGVMSVLRTKIAYIPSVITSMCWLLTQ
KRRPR*NPR**HIST*LKKGGTKASLCSTKYLM*
PKKFKRKLMSSFRSSVQIWTSSLPQSQRPLWTS
G*ALRRVRRSHRSTPSTPSQPYLRTKTLSRSCT
TQ*KKDLEWESRMGD*SVPKRK*LTVRIKWMKTW
SLTRLWMLRK*FSMPRELEASRKSGNPWAHCGR
TSV*VAVLSLACWSSQWPLPRSSSSAW*C*GRG
SMAPSATGSWRLIQCSPQKSVT*TRCRTMAMRTP
PTNTWSRCRFRWQGARQPWRRDAGGPEDPTIPI
DCQAAAAARGCV*HPDLLDCRTI*STTVELQFP
FF*MGEKW*YNNI*YINLK*KK*SIADI*CSFLF
*INQKPHFHCIV*HMLSIYNKWEMSIFNNRLIC
RLSFRLCCVSQLFSLIHCPGFYLKKKKAVFPF*
MSFQEVAEKWGGIGNCNGH*ST*ETLAK*CERTS
FLKSSVSTAGYLCVSIKVLSPRTLKAFYSSVF*
RA*CIQMRVSAAAFLRNPVLPQRLACSLYLTFF
TVCCLPTVAFYRSLPLPTMSRCAGSSSLDFLQRH
*PSASTLSPHLHPLLSPAL*HSLREDHTKEAAA
GPGENTGRFVCVKGK*SRLSLKLSLWTLWKALN
NCVFVTGVFVMLVQLMSMLTASAFSFFLF*I*RF
W*PVVYFYFPVTVFVLFFSFFLPFDPIHVSTHY
AQIKLHLQTP*YDLWRMYTV*THQ*IL*LPPR

Frame 2

YEVSSGWCKTPGAAANWL*MLLGRGVLSEESEC
VSLRAAR*SDPARDGGHRDRGRRSHGQAPASAA
GGAHGACLGAGRLHRGSCSQCRNRICCC*ASNR
NVLWEVKYACEHSDWEMGT*SNRHQELL*NKRRS
SSVLSGDVSRATDHKCDGGKPAG*Y*QLVPEGQ
KAMQESLCYTFQVSRG*ICK*CPASSRKVPVFP
QRADGGV*ESPALAHGSQRGMSDSGNDLI*LRHA
APMWGRPVPWH*ICVLPSDKDYWICVKRRGRGR
*RGRGRGR*RGRL*CL*K*ISY*SRSGRLHRSS
CG*G**G*GRRGGSGGGPRLLL*HLQRR*LQ*GE
SY*TRQRRHHVRQGNYS*CQSCLLPGGDDGALP
GRDASLVLRPLQGKVRALYIWWLRRQQEQF*V*
GLLYGCV*SDDSSNSSANQ*C*CVFRDLCR***A
CSLPEG*GAAGDSAPQPNGQGKEGMGRGRASS*
EPPQSREADSDSALPSHG*SFREGSSQ*EAAAG
GDPPGPSGSYAE*PPSDGSGELPGCLAV*PATAS
SHSPGLTALCPC*EQRSLTYHPSLPACVGC*PR
KGGPDEIPGDDTSPRD*RKEEPKPLSALQSTLC
SPRNSRGN**APSGAACRYGPVHCLNLRDPCGRP
GEL*GE*GDPTVPPLPPLPSPT*ERRHSAGVVP
PNEKRIWSGRAGWGTDRCRRESD*Q*E*SG*KH
GH*RDSGC*GNDFQCRESWRPRGRAGIRGPTAGG
LQSE*QCSHWPAGHRSGHCHGHRHQPGDAEEEA
VWHHQPRDRGG*SNAHPRRASPEQDAEPWL*EP
HLQIPGADADLGGRERGSPGGGMQVGRKIPRFRS
TAKQQPLPGAASDILTSWTVGLYKVLL*NCNFH
SFKWVKNGNITIYDI*TLNEKNDLLQIFDVVFF
FKLIRNPTSIVLSDTCSQYIINGKCRFSIIDLYA
GCRSGYVV*VNSSASFTVLAFI*RKKRQYSLFK
*AFRKLLRNGVE*GTVMATEARERPSQNDVKGP
VS*SPVFPRLDTCVSP*KSCHQGR*RHFIPASSR
ELSVYR*GCPLLLSFGIQCFHRD*PVAYI*HSS
LSVVYLP*LFTVHFPFQLCPDVQAPPLWTFSKG
TDPRPLLCPLTSTPSCHRPCDIHSEKTTPRRRPL
AQERTRGGLFV*KESSPGCP*N*VCGHCGKL*T
IVFSSQESL*CLYS*CRCSLLLLFLSFYFKSEG
SGNLWCIFIFL*LFLFCFFPFSSPLTLFMSLPTM
HRLNFTYKLLNMICGECTQFKHINKYFNFHRD

Sequence APLP2.aAug05

CGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGTGGGGACCGGGCGAACGCCGGAGAGTCGTCTCCTTCGCCCGCCGGAGGAGCGCGGACTGCGGGC
GGGCAGCGGGGTCCGCGGCAGGGCGGGCCGGCGGTGCGGCGCCGGGGGTCCGGCTCTGCGGCGGGTCTGGCGCGCCCTCCCCCGCCCGTCCCTGCTTGGC
GCAGTGCTTAGCGCTGGAGGTCGGGCTGCCTCAGCCGCCGCAGAGGCCAAATGAAAGGCGTCGGGGCTGCTGGGGGCGGCCGTCCGCCTCGGCCGACCCA
GCCCCCAACCCGCCCGCGGCGCCGCCACCTCCTCCGGGGACCCAGTGCCCCCGGGCGCCCGCCCCGCCCTCCCCCGGGACAATGCCAGGGCGCTCCTCTC
CCGCCGGAGTCCGCGGCTGGGCTTTCTCCAGGGGCGGCCCCGCGCGGACCCCGTACGCTCCCTCGCGCGGCACCGGGGCCTCGGCTCCGGGCCTCCCACC
TGCGAGCGGCGGGCTTCGCCTTTGTTGCCAGGTGGACGCGGCCCCGGCCTTCGCGCGCGGCAGGGATGCTGCGAGCCCCGGGGGAGCTCCCGCGCCAGGC
CGCCCGCTGCTCCCTCTGCCGCCTGGGGCCGGGTCGCGGACGCGCATTTTTTAAGTGGCGCTGTTTGCCTGCGTCCGTAGACCGAGGAAACCCGCTCTGG
GCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGA
AATGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATCACAAA
TGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTG
GGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGG
TAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTG
CTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTT
TATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGG
AGGACCGAGATTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAAT
TACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCGGGCCGTGATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGC
GTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTC
TGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCG
CAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAA
GCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTC
GGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAA
AGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTG
ATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGC
GTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTT
CCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAAT
AAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCG
TGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGT
GATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAAC
CATGGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAGGTGGCAGGGAGCGCGGCAGCCCTGGCGGAGGGATGCAGGTGGGCCGGAA
GATCCCACGATTCCGATCGACTGCCAAGCAGCAGCCGCTGCCAGGGGCTGCGTCTGACATCCTGACCTCCTGGACTGTAGGACTATATAAAGTACTACTG
TAGAACTGCAATTTCCATTCTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTATTGCAGATAT
TTGATGTAGTTTTCTTTTTTAAATTAATCAGAAACCCCACTTCCATTGTATTGTCTGACACATGCTCTCAATATATAATAAATGGGAAATGTCGATTTTC
AATAATAGACTTATATGCAGGCTGTCGTTCCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTCCTGGCTTTTATTTAAAGAAAAAAAAGG
CAGTATTCCCTTTTTAAATGAGCTTTCAGGAAGTTGCTGAGAAATGGGGTGGAATAGGGAACTGTAATGGCCACTGAAGCACGTGAGAGACCCTCGCAAA
ATGATGTGAAAGGACCAGTTTCTTGAAGTCCAGTGTTTCCACGGCTGGATACCTGTGTGTCTCCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTT
TATTCCAGCGTCTTCTAGAGAGCTTAGTGTATACAGATGAGGGTGTCCGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGCCTGTAGCT
TATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCTTTTTACCGTTCACTTCCCCTTCCAACTATGTCCAGATGTGCAGGCTCCTCCTCTCT
GGACTTTCTCCAAAGGCACTGACCCTCGGCCTCTACTTTGTCCCCTCACCTCCACCCCCTCCTGTCACCGGCCTTGTGACATTCACTCAGAGAAGACCAC
ACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCTGTCCCTGAAACTGAGTCTGTGGACAC
TGTGGAAAGCTTTGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCTTTCTTTTT
ATTTTAAATCTGAAGGTTCTGGTAACCTGTGGTGTATTTTTATTTTCCTGTGACTGTTTTTGTTTTGTTTTTTTCCTTTTTCCTCCCCTTTGACCCTATT
CATGTCTCTACCCACTATGCACAGATTAAACTTCACCTACAAACTCCTTAATATGATCTGTGGAGAATGTACACAGTTTAAACACATCAATAAATACTTT
AACTTCCACCGAGACT
  • APLP2-F1 (forward primer)
  • APLP2-R1 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

RRLPWRWPATSRWGPGERRRVVSFARRRSADCG
RAAGSAAGRAGGAAPGVRLCGGSGAPSPARPCLA
QCLALEVGLPQPPQRPNERRRGCWGRPSASADP
APNPPAAPPPPPGTQCPRAPAPPSPGTMPGRSS
PAGVRGWAFSRGGPARTPYAPSRGTGASAPGLPP
ASGGLRLCCQVDAAPAFARGRDAASPGGAPAPG
RPLLPLPPGAGSRTRIF*VALFACVRRPRKPAL
GSCSQCRNRICCC*ASNRNVLWEVKYACEHSDWE
MGT*SNRHQELL*NKRRSSSVLSGDVSRATDHK
CDGGKPAG*Y*QLVPEGQKAMQESLCYTFQVSR
G*ICK*CPASSRKVPVFPQRADGGV*ESPALAHG
SQRGMSDSGNDLI*LRHAAPMWGRPVPWH*ICV
LPSDKDYWICVKRRGRGR*RGRGRGR*RGRL*C
L*K*ISY*SRSGRLHRSSCG*G**G*GRRGGSGG
GPRLLL*HLQRR*LQ*GESY*TRQRRHHVRQGN
YS*CQSCLLPGGDDGALPGRDASLVLRPLQGKV
RALYIWWLRRQQEQF*V*GLLYGCV*SDDSSNSS
ANQ*C*CVFRDLCR***ACSLPEG*GAAGDSAP
QPNGQGKEGMGRGRASS*EPPQSREADSDSALP
SHG*SFREGSSQ*EAAAGGDPPGPSGSYAE*PPS
DGSGELPGCLAV*PATASSHSPGLTALCPC*EQ
RSLTYHPSLPACVGC*PRKGGPDEIPGDDTSPR
D*RKEEPKPLSALQSTLCSPRNSRGN**APSGAA
CRYGPVHCLNLRDPCGRPGEL*GE*GDPTVPPL
PPLPSPT*ERRIWSGRAGWGTDRCRRESD*Q*E
*SG*KHGH*RDSGC*GNDFQCRESWRPRGRAGIR
GPTAGGLQSE*QCSHWPAGHRSGHCHGHRHQPG
DAEEEAVWHHQPRDRGG*SNAHPRRASPEQDAE
PWL*EPHLQIPGADADLGGRERGSPGGGMQVGRK
IPRFRSTAKQQPLPGAASDILTSWTVGLYKVLL
*NCNFHSFKWVKNGNITIYDI*TLNEKNDLLQI
FDVVFFFKLIRNPTSIVLSDTCSQYIINGKCRFS
IIDLYAGCRSGYVV*VNSSASFTVLAFI*RKKR
QYSLFK*AFRKLLRNGVE*GTVMATEARERPSQ
NDVKGPVS*SPVFPRLDTCVSP*KSCHQGR*RHF
IPASSRELSVYR*GCPLLLSFGIQCFHRD*PVA
YI*HSSLSVVYLP*LFTVHFPFQLCPDVQAPPL
WTFSKGTDPRPLLCPLTSTPSCHRPCDIHSEKTT
PRRRPLAQERTRGGLFV*KESSPGCP*N*VCGH
CGKL*TIVFSSQESL*CLYS*CRCSLLLLFLSF
YFKSEGSGNLWCIFIFL*LFLFCFFPFSSPLTLF
MSLPTMHRLNFTYKLLNMICGECTQFKHINKYF
NFHRD

Frame 1

GACLGAGRLHRGGDRANAGESSPSPAGGARTAG
GQRGPRQGGPAVRRRGSGSAAGLARPPPPVPAW
RSA*RWRSGCLSRRRGQMKGVGAAGGGRPPRPTQ
PPTRPRRRHLLRGPSAPGRPPRPPPGQCQGAPL
PPESAAGLSPGAAPRGPRTLPRAAPGPRLRASH
LRAAGFAFVARWTRPRPSRAAGMLRAPGELPRQA
ARCSLCRLGPGRGRAFFKWRCLPASVDRGNPLW
ALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTG
KWEPDPTGTKSCFETKEEVLQYCQEMYPELQITN
VMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLV
GEFVSDVLLVPEKCQFFHKERMEVCENHQHWHT
VVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVC
CPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDV
YKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVV
EDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEI
THDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKC
VRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTP
LPTNDVDVYFETSADDNEHARFQKAKEQLEIRHR
NRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQ
AMVKALEKEAASEKQQLVETHLARVEAMLNDRR
RMALENYLAALQSDPPRPHRILQALRRYVRAENK
DRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHV
IEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQ
RADMDQFTASISETPVDVRVSSEESEEIPPFHPF
HPFPALPENEGSGVGEQDGGLIGAEEKVINSKN
KVDENMVIDETLDVKEMIFNAERVGGLEEERES
VGPLREDFSLSSSALIGLLVIAVAIATVIVISLV
MLRKRQYGTISHGIVEVDPMLTPEERHLNKMQN
HGYENPTYKYLEQMQI*VAGSAAALAEGCRWAG
RSHDSDRLPSSSRCQGLRLTS*PPGL*DYIKYYC
RTAISILLNG*KMVI*QYMIYKP*MKKMIYCRY
LM*FSFLN*SETPLPLYCLTHALNI**MGNVDF
Q**TYMQAVVPVMLCKSTLQPHSLSWLLFKEKKG
SIPFLNELSGSC*EMGWNREL*WPLKHVRDPRK
MM*KDQFLEVQCFHGWIPVCLHKSPVTKDVKGI
LFQRLLESLVYTDEGVRCCFPSESSASTEISL*L
IFDILHCLLFTYRSFLPFTSPSNYVQMCRLLLS
GLSPKALTLGLYFVPSPPPPPVTGLVTFTQRRP
HQGGGRWPRREHGEVCLCERKVVQAVPETESVDT
VESFEQLCFRHRSLCNACTVDVDAHCFCFFFLF
ILNLKVLVTCGVFLFSCDCFCFVFFLFPPL*PY
SCLYPLCTD*TSPTNSLI*SVENVHSLNTSINTL
TSTET

Frame 2

APALALAGYIEVGTGRTPESRLLRPPEERGLRA
GSGVRGRAGRRCGAGGPALRRVWRALPRPSLLG
AVLSAGGRAASAAAEAK*KASGLLGAAVRLGRP
SPQPARGAATSSGDPVPPGARPALPRDNARALLS
RRSPRLGFLQGRPRADPVRSLARHRGLGSGPPT
CERRASPLLPGGRGPGLRARQGCCEPRGSSRAR
PPAAPSAAWGRVADAHFLSGAVCLRP*TEETRSG
LLQPMPEQDLLLLSLKSQCFVGS*ICM*TFRLG
NGNLIQQAPRAALKQKKKFFSTVRRCIQSYRSQ
M*WRQTSGLVLTTGAGGTKSNARVALLHLSSVSW
VNL*VMSC*FQKSASFSTKSGWRCVRITSTGTR
*SKRHV*LRE*PYIATACCSHVG*TSSMALNMC
AALRQRLLDLCQKKRKRKMKRKRKRKMKRKTMMF
IKVNFLLKQIWKTSQKQLWMRMMRMRKKGRKWW
RTEITTMTPSKEMTTMRRILLNPAATAPCQTRK
LLMMSKLSAPRRR*RGPAGP*CLVGTSTSPRESA
CALYMVAAAATGTILSLRIIVWLCVKR*FLQLL
CQPMMLMCISRPLQMIMSMLASRRLRSSWRFGT
ATEWTG*RRNGKRQSFKLRTSPKQRGRL*FSTSK
PWLKL*RRKQPVRSSSWWRPTWPEWKLC*MTAV
GWLWRTTWLPCSLTRHGLIAFSRPYGVMSVLRT
KIAYIPSVITSMCWLLTQKRRPR*NPR**HIST*
LKKGGTKASLCSTKYLM*PKKFKRKLMSSFRSS
VQIWTSSLPQSQRPLWTSG*ALRRVRRSHRSTP
STPSQPYLRTKDLEWESRMGD*SVPKRK*LTVRI
KWMKTWSLTRLWMLRK*FSMPRELEASRKSGNP
WAHCGRTSV*VAVLSLACWSSQWPLPRSSSSAW
*C*GRGSMAPSATGSWRLIQCSPQKSVT*TRCRT
MAMRTPPTNTWSRCRFRWQGARQPWRRDAGGPE
DPTIPIDCQAAAAARGCV*HPDLLDCRTI*STT
VELQFPFF*MGEKW*YNNI*YINLK*KK*SIADI
*CSFLF*INQKPHFHCIV*HMLSIYNKWEMSIF
NNRLICRLSFRLCCVSQLFSLIHCPGFYLKKKK
AVFPF*MSFQEVAEKWGGIGNCNGH*ST*ETLAK
*CERTSFLKSSVSTAGYLCVSIKVLSPRTLKAF
YSSVF*RA*CIQMRVSAAAFLRNPVLPQRLACS
LYLTFFTVCCLPTVAFYRSLPLPTMSRCAGSSSL
DFLQRH*PSASTLSPHLHPLLSPAL*HSLREDH
TKEAAAGPGENTGRFVCVKGK*SRLSLKLSLWT
LWKALNNCVFVTGVFVMLVQLMSMLTASAFSFFL
F*I*RFW*PVVYFYFPVTVFVLFFSFFLPFDPI
HVSTHYAQIKLHLQTP*YDLWRMYTV*THQ*IL
*LPPR

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