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Sequences

Sequence IRAK1.iAug05

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

FVTQTLWTLLATVLRTASTAWCTASCPTAPWRT VSTARPRPAHLSPGLSDWTSFWVQPGQFSFYIRT APASSMETSRVPTSFWMRG*HPSWETLAWPGSA ALPGPAPARAAWWPGHRQCGAPWPTCPRSTSRR EGWLWTRTPSALGW*C*RPWLVRGL*RRTVPGPS I*KTWWKRRLRRLEWL*EAPRAHCKQVWLQMPG LLPSPCRSTRSAWASWPAAACTAGPKGGLL*PR RTPTCPALAEPTVGLLHGSPWQRHQEPVPRQQSS CREAPTSPWRVTRA*AASLLPCAPGT*LQAALW TQHPSGRPAVLRGTRQE

Frame 1

SSPKHCGLCWLLCSERLLLPGVRLPAQRLPGGP SPLPDPGLPTSLLASATGHPSGYSPGNSVSTSG QPQPHPWRHQEFQRPSG*EADTQAGRLWPGPVQP LCRVQPQPEQHGGPDTDSAGHPGLPARGVHQDG KAGCGHGHLQLWGGSARDLGWSEGCEDARCQDQ VSERPGGRGG*GGWSGFEKHPEHTASRSGCRCLG CSHRHADLQEAPGPAGLLLPAPPGQKEASYDPG ELLRVQHWQSPQWGCSMAAPGSAIRSQCPGSRA AAERPQPARGE*REPRRPLCCPALLALDSKLPSG PSTPQGGRLSSGGHGRR

Frame 2

RHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDR LHCQTQACPPLSWPQRLDILLGTARAIQFLHQD SPSLIHGDIKSSNVLLDERLTPKLGDFGLARFS RFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTG RLAVDTDTFSFGVVVLETLAGQRAVKTHGARTK YLKDLVEEEAEEAGVALRSTQSTLQAGLAADAW AAPIAMQIYKKRLGQLACCCLHRRAKRRPPMTQE NSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQ LQRGPNQPVESDESLGGLSAALRSWHLTPSCPL DPAPLREAGCPQGDTAG

Sequence IRAK1.cAug05

GCGCAAGATGGCGGCGGGCCCGGGCACCGCCCCTTCCGCCCCGCCGGGCGTCGCACGAGGCCGGCTCGAAGGGGAAGTGAGTCAGTGTCCGCGGACCCGG CCGGCCCAGGCCCGCGCCCGCCGCGGCCCTGAGAGGCCCCGGCAGGTCCCGGCCCGGCGGCGGCAGCCATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGC AGCCCCCGGCGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAG TTCGCCGCCCTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACG CCCGTGTGGCCGACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCC AGGCACCACTGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCC CCAGCTTTTCCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTT CTACCAAGCCAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTTTCCGTTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAA CTTCTCGGAGGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAAC GCTGACCTGGAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACT GTGCTCAGAACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTC CTGGCCTCAGCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCC AACGTCCTTCTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGG TGGCCCGGACACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGG GGTGGTAGTGCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAG GCTGGAGTGGCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACC TGGACCCCAGGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTAT GACCCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCA GCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCT GCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGC CGTGGAAGGACTGGCCCTTGGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCC CTGTACGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTG ATGAATTTCAGAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAAGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCA CTCTGCCGGCAGTGGGGGTGGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGGAACC CTGCCTCCATGCCAGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATGTTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGG GAGGAGAGGGGCTGCTGTGCAGGGGTGTGGAGTAGGGAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTG CCCATCTCTTTGGACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTTGGAAAAGTGTGGCCGGGTGCGGTG GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGATCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTC TCTGCCAAAAAATTTTTTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGATCATTTATGCTTGG GAGGTCGAGGCTGCAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACAGAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAA GTATGGCTGTAAGTCTCATGGTTCAGTCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCAGTGT CTTCAGGCTTTACTGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTGGGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCT TCGGAGGCCACAAGCTCAGCCTCAGGCCCAGGCACTGATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGT GAGAAGCCCCTGGAAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGTATCACATGTCTTCAGAAGCAAGTC AGGTTTCATGTAACCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCCAGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTA CATGCCCCCGTGAAGCTGGGCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAACGTATGTTGCAATCTCGGGCTCTACTTGTGGACTTTGTTGCAC CGAAAGCCTTGAGCTTTCCTGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

AQDGGGPGHRPFRPAGRRTRPARRGSESVSADP AGPGPRPPRP*EAPAGPGPAAAAMAGGPGPGEPA APGAQHFLYEVPPWVMCRFYKVMDALEPADWCQ FAALIVRDQTELRLCERSGQRTASVLWPWINRN ARVADLVHILTHLQLLRARDIITAWHPPAPLPSP GTTAPRPSSIPAPAEAEAWSPRKLPSSASTFLS PAFPGSQTHSGPELGLVPSPASLWPPPPSPAPS STKPGPESSVSLLQGARPFPFCWPLCEISRGTHN FSEELKIGEGGFGCVYRAVMRNTVYAVKRLKEN ADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGY CAQNGFYCLVYGFLPNGSLEDRLHCQTQACPPLS WPQRLDILLGTARAIQFLHQDSPSLIHGDIKSS NVLLDERLTPKLGDFGLARFSRFAGSSPSQSSM VARTQTVRGTLAYLPEEYIKTGRLAVDTDTFSFG VVVLETLAGQRAVKTHGARTKYLKDLVEEEAEE AGVALRSTQSTLQAGLAADAWAAPIAMQIYKKH LDPRPGPCPPELGLGLGQLACCCLHRRAKRRPPM TQENSYVSSTGRAHSGAAPWQPLAAPSGASAQA AEQLQRGPNQPVESDESLGGLSAALRSWHLTPS CPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTA VEGLALGSSASSSSEPPQIIINPARQKMVQKLA LYEDGALDSLQLLSSSSLPGLGLEQDRQGPEES DEFQS*CVHLGRSPKSGSQSSHGQKFSWCTSPQH SAGSGGGGPCPRGREGGGPAVLGSVGIGRQSGT LPPCQHLGARKAGIIQ*GGWRMLGGCWLHRPVR GGEGLLCRGVE*GAGSPESHAGRLQPRPLAAALC PSLWTVATLHNGADEA*GPPTCLQFGKVWPGAV AHACNPSTLGGQGRRIAGAQ*VKTSQGNMMRPC LCQKIF*TISLAW*RTPVVPAAGEAEVGGSFMLG RSRLQ*VMIV*LHSSLGDRARPCFKKKNPGKSE VWL*VSWFSPSKKREF*DPPESRAAPTSNLGPV SSGFTGDLRAGLMWWPASQAIPGRHRRAPSQVRL RRPQAQPQAQALIVAEGPLPKV*LGPRPSYPDS EKPLEGRKVGSMADREGKHFQGKDMYHMSSEAS QVSCNRVSSCVSKSSPGL*HRLHSDFVFSRESHY MPP*SWALVTSRLSLSNKNVCCNLGLYLWTLLH RKP*AFLMPYTSGFLSV

Frame 1

RKMAAGPGTAPSAPPGVARGRLEGEVSQCPRTR PAQARARRGPERPRQVPARRRQPWPGGRARGSP QPPAPSTSCTRCRPGSCAASTK*WTPWSPPTGAS SPP*SCATRPSCGCASAPGSARPASCGPGSTAT PVWPTSCTSSRTCSCSVRGTSSQPGTLPPRFRP QAPLPRGPAASLHPPRPRPGAPGSCHPQPPPSSP QLFQAPRPIQGLSSAWSQALLPCGLHRHLQPLL LPSQAQRAQCPSCREPAPFRFAGPSVRFPGAPT TSRRSSRSGRVALGACTGR**GTRCML*RG*RRT LTWSGLQ*SRAS*PRWSSCPGFVTQTLWTLLAT VLRTASTAWCTASCPTAPWRTVSTARPRPAHLS PGLSDWTSFWVQPGQFSFYIRTAPASSMETSRVP TSFWMRG*HPSWETLAWPGSAALPGPAPARAAW WPGHRQCGAPWPTCPRSTSRREGWLWTRTPSAL GW*C*RPWLVRGL*RRTVPGPSI*KTWWKRRLRR LEWL*EAPRAHCKQVWLQMPGLLPSPCRSTRST WTPGPGPAHLSWAWAWASWPAAACTAGPKGGLL *PRRTPTCPALAEPTVGLLHGSPWQRHQEPVPRQ QSSCREAPTSPWRVTRA*AASLLPCAPGT*LQA ALWTQHPSGRPAVLRGTRQENRAGGVAQDPGPQ PWKDWPLAALHHRRQSHRRLSSTLPDRRWSRSWP CTRMGPWTACSCCRPAPSQAWAWNRTGRGPKKV MNFRADVFTWADPPNPEVKVLMVRSSHGARVLS TLPAVGVGAHARGGEKEVALLF*ALWA*AGRVEP CLHASIWGQGRLASSSEEAGACWEAAGCTDP*G EERGCCAGVWSRELAPLRAMQGVCSPGLWQQLF AHLFGQWPPCTMGPTRPRALLPAYNLEKCGRVRW LTPVIPALWEAKAGGSLEPSRSRPARAT**DPV SAKKFFKLLAWRGSARLWSQLLGRLK*EDHLCL GGRGCSES*LYDCTPAWVTEQDPVSKRKTLGKVK YGCKSHGSVLARSENSEILQKVEQHPPPTSGQC LQALLGTCELA*CGGLQARPSLGATDELRARSG FGGHKLSLRPRH*LWQRGHYPRSS*AQDLVTQTV RSPWKAEKLGAWQTGKGNIFREKTCITCLQKQV RFHVTECPLACPKVAQGCSTGFTVILCSAVSHT TCPREAGHW*RPGCP*VIKTYVAISGSTCGLCCT ESLELS*CLTLQGS*AS

Frame 2

ARWRRARAPPLPPRRASHEAGSKGK*VSVRGPG RPRPAPAAALRGPGRSRPGGGSHGRGAGPGGAR SPRRPALLVRGAALGHVPLLQSDGRPGARRLVP VRRPDRARPDRAAAVRALRAAHGQRPVALDQPQR PCGRPRAHPHAPAAAPCAGHHHSLAPSRPASVP RHHCPEAQQHPCTRRGRGLEPPEVAILSLHLPL PSFSRLPDPFRA*ARPGPKPCFPVASTAISSPFF YQARPRELSVPPAGSPPLSVLLAPL*DFPGHPQ LLGGAQDRGGWLWVRVPGGDEEHGVCCEEAEGE R*PGVDCSEAELPDRGGAAVQVSSPKHCGLCWLL CSERLLLPGVRLPAQRLPGGPSPLPDPGLPTSL LASATGHPSGYSPGNSVSTSGQPQPHPWRHQEF QRPSG*EADTQAGRLWPGPVQPLCRVQPQPEQHG GPDTDSAGHPGLPARGVHQDGKAGCGHGHLQLW GGSARDLGWSEGCEDARCQDQVSERPGGRGG*G GWSGFEKHPEHTASRSGCRCLGCSHRHADLQEAP GPQARALPT*AGPGPGPAGLLLPAPPGQKEASY DPGELLRVQHWQSPQWGCSMAAPGSAIRSQCPG SRAAAERPQPARGE*REPRRPLCCPALLALDSKL PSGPSTPQGGRLSSGGHGRRIELGEWPRIPAHS RGRTGPWQLCIIVVRATADYHQPCPTEDGPEAG PVRGWGPGQPAAAVVQLPPRLGPGTGQAGARRK* *ISELMCSPGQIPQIRKSKFSWSEVLMVHESSA LCRQWGWGPMPAGERRRWPCCSRLCGHRQAEWN PASMPASGGKEGWHHPVRRLAHVGRLLAAQTREG RRGAAVQGCGVGSWLP*EPCRASAAQASGSSSL PISLDSGHPAQWGRRGLGPSYLLTIWKSVAGCG GSRL*SQHFGRPRQEDRWSPVGQDQPGQHDETLS LPKNFLNY*PGVVAHACGPSCWGG*SRRIIYAW EVEAAVSHDCMTALQPG*QSKTLFQKEKPWEK* SMAVSLMVQS*QEARILRSSRKSSSTHLQPRASV FRLYWGPASWPNVVACKPGHPWAPQTSSEPGQA SEATSSASGPGTDCGRGATTQGLARPKT*LPRQ *EAPGRQKSWEHGRQGRETFSGKRHVSHVFRSKS GFM*PSVLLRVQK*PRAVAQASQ*FCVQP*VTL HAPVKLGIGDVQVVLE**KRMLQSRALLVDFVA PKALSFPDALHFRVLER

Sequence IRAK1.bAug05

GCGGACCCGGCCGGCCCAGGCCCGCGCCCGCCGCGGCCCTGAGAGGCCCCGGCAGGTCCCGGCCCGGCGGCGGCAGCCATGGCCGGGGGGCCGGGCCCGG GGGAGCCCGCAGCCCCCGGCGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGA CTGGTGCCAGTTCGGTGGGTGGCGGCGGGCTGCCGGGGGGCGGGAGGCGCGCGGGCTCCTGGCGCCGACGCCTGACGCCCCCCGCCCCGCAGCCGCCCTG ATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACGCCCGTGTGGCCG ACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCCAGGCACCACTGC CCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCCCCAGCTTTTCCA GGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTTCTACCAAGCCAG GCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTTTCCGTTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGAGGA GCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAACGCTGACCTGGAG TGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGAACG GCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCG ACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTTCTG GATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGACAC AGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGTGCT AGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCT TTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGGCCAGCTGG CCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCC ATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGC GGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAG GAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTTGGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGAT TATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCA GGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAA GTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGCAGTGGGGGTGGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCC TGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGGAACCCTGCCTCCATGCCAGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGC TGGCGCATGTTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGGGCTGCTGTGCAGGGGTGTGGAGTAGGGAGCTGGCTCCCCTGAGAGCC ATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATCTCTTTGGACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCC TCCTACCTGCTTACAATTTGGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGATCGCTGGAGCC CAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAAAATTTTTTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCC CAGCTGCTGGGGAGGCTGAAGTAGGAGGATCATTTATGCTTGGGAGGTCGAGGCTGCAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACAG AGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGTAAGTCTCATGGTTCAGTCCTAGCAAGAAGCGAGAATTCTGAGATCCT CCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCAGTGTCTTCAGGCTTTACTGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGG CCATCCCTGGGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCACAAGCTCAGCCTCAGGCCCAGGCACTGATTGTGGCAGAGGGGCCACT ACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAGCCCCTGGAAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACAT TTTCAGGGAAAAGACATGTATCACATGTCTTCAGAAGCAAGTCAGGTTTCATGTAACCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCCAGGGCTGTAGC ACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCGTGAAGCTGGGCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAA CGTATGTTGCAATCTCGGGCTCTACTTGTGGACTTTGTTGCACCGAAAGCCTTGAGCTTTCCTGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

ADPAGPGPRPPRP*EAPAGPGPAAAAMAGGPGP GEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPAD WCQFGGWRRAAGGREARGLLAPTPDAPRPAAAL IVRDQTELRLCERSGQRTASVLWPWINRNARVA DLVHILTHLQLLRARDIITAWHPPAPLPSPGTTA PRPSSIPAPAEAEAWSPRKLPSSASTFLSPAFP GSQTHSGPELGLVPSPASLWPPPPSPAPSSTKP GPESSVSLLQGARPFPFCWPLCEISRGTHNFSEE LKIGEGGFGCVYRAVMRNTVYAVKRLKENADLE WTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQN GFYCLVYGFLPNGSLEDRLHCQTQACPPLSWPQR LDILLGTARAIQFLHQDSPSLIHGDIKSSNVLL DERLTPKLGDFGLARFSRFAGSSPSQSSMVART QTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVVL ETLAGQRAVKTHGARTKYLKDLVEEEAEEAGVA LRSTQSTLQAGLAADAWAAPIAMQIYKKHLGQL ACCCLHRRAKRRPPMTQENSYVSSTGRAHSGAAP WQPLAAPSGASAQAAEQLQRGPNQPVESDESLG GLSAALRSWHLTPSCPLDPAPLREAGCPQGDTA GESSWGSGPGSRPTAVEGLALGSSASSSSEPPQI IINPARQKMVQKLALYEDGALDSLQLLSSSSLP GLGLEQDRQGPEESDEFQS*CVHLGRSPKSGSQ SSHGQKFSWCTSPQHSAGSGGGGPCPRGREGGGP AVLGSVGIGRQSGTLPPCQHLGARKAGIIQ*GG WRMLGGCWLHRPVRGGEGLLCRGVE*GAGSPES HAGRLQPRPLAAALCPSLWTVATLHNGADEA*GP PTCLQFGKVWPGAVAHACNPSTLGGQGRRIAGA Q*VKTSQGNMMRPCLCQKIF*TISLAW*RTPVV PAAGEAEVGGSFMLGRSRLQ*VMIV*LHSSLGDR ARPCFKKKNPGKSEVWL*VSWFSPSKKREF*DP PESRAAPTSNLGPVSSGFTGDLRAGLMWWPASQ AIPGRHRRAPSQVRLRRPQAQPQAQALIVAEGPL PKV*LGPRPSYPDSEKPLEGRKVGSMADREGKH FQGKDMYHMSSEASQVSCNRVSSCVSKSSPGL* HRLHSDFVFSRESHYMPP*SWALVTSRLSLSNKN VCCNLGLYLWTLLHRKP*AFLMPYTSGFLSV

Frame 1

RTRPAQARARRGPERPRQVPARRRQPWPGGRAR GSPQPPAPSTSCTRCRPGSCAASTK*WTPWSPP TGASSVGGGGLPGGGRRAGSWRRRLTPPAPQPP* SCATRPSCGCASAPGSARPASCGPGSTATPVWP TSCTSSRTCSCSVRGTSSQPGTLPPRFRPQAPL PRGPAASLHPPRPRPGAPGSCHPQPPPSSPQLFQ APRPIQGLSSAWSQALLPCGLHRHLQPLLLPSQ AQRAQCPSCREPAPFRFAGPSVRFPGAPTTSRR SSRSGRVALGACTGR**GTRCML*RG*RRTLTWS GLQ*SRAS*PRWSSCPGFVTQTLWTLLATVLRT ASTAWCTASCPTAPWRTVSTARPRPAHLSPGLS DWTSFWVQPGQFSFYIRTAPASSMETSRVPTSFW MRG*HPSWETLAWPGSAALPGPAPARAAWWPGH RQCGAPWPTCPRSTSRREGWLWTRTPSALGW*C *RPWLVRGL*RRTVPGPSI*KTWWKRRLRRLEWL *EAPRAHCKQVWLQMPGLLPSPCRSTRSTWASW PAAACTAGPKGGLL*PRRTPTCPALAEPTVGLL HGSPWQRHQEPVPRQQSSCREAPTSPWRVTRA*A ASLLPCAPGT*LQAALWTQHPSGRPAVLRGTRQ ENRAGGVAQDPGPQPWKDWPLAALHHRRQSHRR LSSTLPDRRWSRSWPCTRMGPWTACSCCRPAPSQ AWAWNRTGRGPKKVMNFRADVFTWADPPNPEVK VLMVRSSHGARVLSTLPAVGVGAHARGGEKEVA LLF*ALWA*AGRVEPCLHASIWGQGRLASSSEEA GACWEAAGCTDP*GEERGCCAGVWSRELAPLRA MQGVCSPGLWQQLFAHLFGQWPPCTMGPTRPRA LLPAYNLEKCGRVRWLTPVIPALWEAKAGGSLEP SRSRPARAT**DPVSAKKFFKLLAWRGSARLWS QLLGRLK*EDHLCLGGRGCSES*LYDCTPAWVT EQDPVSKRKTLGKVKYGCKSHGSVLARSENSEIL QKVEQHPPPTSGQCLQALLGTCELA*CGGLQAR PSLGATDELRARSGFGGHKLSLRPRH*LWQRGH YPRSS*AQDLVTQTVRSPWKAEKLGAWQTGKGNI FREKTCITCLQKQVRFHVTECPLACPKVAQGCS TGFTVILCSAVSHTTCPREAGHW*RPGCP*VIK TYVAISGSTCGLCCTESLELS*CLTLQGS*AS

Frame 2

GPGRPRPAPAAALRGPGRSRPGGGSHGRGAGPG GARSPRRPALLVRGAALGHVPLLQSDGRPGARR LVPVRWVAAGCRGAGGARAPGADA*RPPPRSRP DRARPDRAAAVRALRAAHGQRPVALDQPQRPCGR PRAHPHAPAAAPCAGHHHSLAPSRPASVPRHHC PEAQQHPCTRRGRGLEPPEVAILSLHLPLPSFS RLPDPFRA*ARPGPKPCFPVASTAISSPFFYQAR PRELSVPPAGSPPLSVLLAPL*DFPGHPQLLGG AQDRGGWLWVRVPGGDEEHGVCCEEAEGER*PG VDCSEAELPDRGGAAVQVSSPKHCGLCWLLCSER LLLPGVRLPAQRLPGGPSPLPDPGLPTSLLASA TGHPSGYSPGNSVSTSGQPQPHPWRHQEFQRPS G*EADTQAGRLWPGPVQPLCRVQPQPEQHGGPDT DSAGHPGLPARGVHQDGKAGCGHGHLQLWGGSA RDLGWSEGCEDARCQDQVSERPGGRGG*GGWSG FEKHPEHTASRSGCRCLGCSHRHADLQEAPGPAG LLLPAPPGQKEASYDPGELLRVQHWQSPQWGCS MAAPGSAIRSQCPGSRAAAERPQPARGE*REPR RPLCCPALLALDSKLPSGPSTPQGGRLSSGGHGR RIELGEWPRIPAHSRGRTGPWQLCIIVVRATAD YHQPCPTEDGPEAGPVRGWGPGQPAAAVVQLPP RLGPGTGQAGARRK**ISELMCSPGQIPQIRKSK FSWSEVLMVHESSALCRQWGWGPMPAGERRRWP CCSRLCGHRQAEWNPASMPASGGKEGWHHPVRR LAHVGRLLAAQTREGRRGAAVQGCGVGSWLP*EP CRASAAQASGSSSLPISLDSGHPAQWGRRGLGP SYLLTIWKSVAGCGGSRL*SQHFGRPRQEDRWS PVGQDQPGQHDETLSLPKNFLNY*PGVVAHACGP SCWGG*SRRIIYAWEVEAAVSHDCMTALQPG*Q SKTLFQKEKPWEK*SMAVSLMVQS*QEARILRS SRKSSSTHLQPRASVFRLYWGPASWPNVVACKPG HPWAPQTSSEPGQASEATSSASGPGTDCGRGAT TQGLARPKT*LPRQ*EAPGRQKSWEHGRQGRET FSGKRHVSHVFRSKSGFM*PSVLLRVQK*PRAVA QASQ*FCVQP*VTLHAPVKLGIGDVQVVLE**K RMLQSRALLVDFVAPKALSFPDALHFRVLER

Sequence IRAK1.nAug05

GGCCACCTGGTAGCCATACTGACAGTATTTATCCCACAGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAGCA CACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGACCCCAGGCCCGGGCCCTGCCCACCTGA GCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGTGTACGAGAGGCTAGAGAAGCTG CAGGCAGTGGTGGCGGGGGTGCCCGGGCATTCGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGCCC ACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAGAG TGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTCCT CAGGGGGACACGGCAGGAGA

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

GHLVAILTVFIPQKDLVEEEAEEAGVALRSTQS TLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPE LGLGLGQLACCCLHRRAKRRPPMTQVYERLEKL QAVVAGVPGHSEAASCIPPSPQENSYVSSTGRA HSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVES DESLGGLSAALRSWHLTPSCPLDPAPLREAGCP QGDTAG

Frame 1

ATW*PY*QYLSHRKTWWKRRLRRLEWL*EAPRA HCKQVWLQMPGLLPSPCRSTRSTWTPGPGPAHL SWAWAWASWPAAACTAGPKGGLL*PRCTRG*RSC RQWWRGCPGIRRPPAASPLPRRRTPTCPALAEP TVGLLHGSPWQRHQEPVPRQQSSCREAPTSPWR VTRA*AASLLPCAPGT*LQAALWTQHPSGRPAVL RGTRQE

Frame 2

PPGSHTDSIYPTERPGGRGG*GGWSGFEKHPEH TASRSGCRCLGCSHRHADLQEAPGPQARALPT* AGPGPGPAGLLLPAPPGQKEASYDPGVREAREA AGSGGGGARAFGGRQLHPPFPAGELLRVQHWQSP QWGCSMAAPGSAIRSQCPGSRAAAERPQPARGE *REPRRPLCCPALLALDSKLPSGPSTPQGGRLS SGGHGRR

Sequence IRAK1.hAug05

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

SRQGGRNSAFDVAQSPSQPSWVSWEAAVVGRAW SLGQKGRDRDPT*SRLSSHSQAQRAQCPSCREPA PFRFAGPSVRFPGAPTTSRRSSRSGRVALGACT GR**GTRCML*RG*RRTLTWSGLQ*SRAS*PRW SSCPGFVTQTLWTLLATVLRTASTAWCTASCPTA PWRTVSTARPRPAHLSPGLSDWTSFWVQPGQFS FYIRTAPASSMETSRVPTSFWMRG*HPSWETLA WPGSAALPGPAPARAAWWPGHRQCGAPWPTCPRS TSRREGWLWTRTPSALG*KTWWKRRLRRLEWL* EAPRAHCKQVWLQMPGLLPSPCRSTRSTWTPGP GPAHLSWAWAWASWPAAACTAGPKGGLL*PRCTR G*RSCRQWWRGCPGIRRPPAASPLPRRRTPTCP ALAEPTVGLLHGSPWQRHQEPVPRQQSSCREAP TSPWRVTRA*AASLLPCAPGT*LQAALWTQHPSG RPAVLRGTRQE

Frame 1

RDKEEGIQPLM*RRAPVSQAGSAGRQLWWGEPG ALGRREETGTPPDPGSLPTARPRELSVPPAGSP PLSVLLAPL*DFPGHPQLLGGAQDRGGWLWVRVP GGDEEHGVCCEEAEGER*PGVDCSEAELPDRGG AAVQVSSPKHCGLCWLLCSERLLLPGVRLPAQR LPGGPSPLPDPGLPTSLLASATGHPSGYSPGNSV STSGQPQPHPWRHQEFQRPSG*EADTQAGRLWP GPVQPLCRVQPQPEQHGGPDTDSAGHPGLPARG VHQDGKAGCGHGHLQLWGERPGGRGG*GGWSGFE KHPEHTASRSGCRCLGCSHRHADLQEAPGPQAR ALPT*AGPGPGPAGLLLPAPPGQKEASYDPGVR EAREAAGSGGGGARAFGGRQLHPPFPAGELLRVQ HWQSPQWGCSMAAPGSAIRSQCPGSRAAAERPQ PARGE*REPRRPLCCPALLALDSKLPSGPSTPQ GGRLSSGGHGRR

Frame 2

ETRRKEFSL*CSAEPQSAKLGQLGGSCGGESLE PWAEGKRQGPHLIQALFPQPGPESSVSLLQGAR PFPFCWPLCEISRGTHNFSEELKIGEGGFGCVY RAVMRNTVYAVKRLKENADLEWTAVKQSFLTEVE QLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNG SLEDRLHCQTQACPPLSWPQRLDILLGTARAIQ FLHQDSPSLIHGDIKSSNVLLDERLTPKLGDFGL ARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEE YIKTGRLAVDTDTFSFGVKDLVEEEAEEAGVAL RSTQSTLQAGLAADAWAAPIAMQIYKKHLDPRPG PCPPELGLGLGQLACCCLHRRAKRRPPMTQVYE RLEKLQAVVAGVPGHSEAASCIPPSPQENSYVS STGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPN QPVESDESLGGLSAALRSWHLTPSCPLDPAPLR EAGCPQGDTAG

Sequence IRAK1.gAug05

TTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCG TCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATCAGGAC AGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCC GCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAGACGGG AAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAG TATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGG CTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGAC CCAGGTGTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTCGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAAC TCCTACGTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGC AGAGAGGCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCC AGCACCCCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTG GCCCTTGGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATG GGGCCCTGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAG CTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAAGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGCAGT GGGGGTGGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGGAACCCTGCCTCCATGCC AGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATGTTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGGGCT GCTGTGCAGGGGTGTGGAGTAGGGAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATCTCTTTGG ACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTTGGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCTGTA ATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGATCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAAAAT TTTTTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGATCATTTATGCTTGGGAGGTCGAGGCTG CAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACAGAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGTAAG TCTCATGGTTCAGTCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCAGTGTCTTCAGGCTTTAC TGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTGGGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCACAA GCTCAGCCTCAGGCCCAGGCACTGATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAGCCCCTGG AAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGTATCACATGTCTTCAGAAGCAAGTCAGGTTTCATGTAA CCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCCAGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCGTGA AGCTGGGCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAACGTATGTTGCAATCTCGGGCTCTACTTGTGGACTTTGTTGCACCGAAAGCCTTGAG CTTTCCTGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

FVTQTLWTLLATVLRTASTAWCTASCPTAPWRT VSTARPRPAHLSPGLSDWTSFWVQPGQFSFYIRT APASSMETSRVPTSFWMRG*HPSWETLAWPGSA ALPGPAPARAAWWPGHRQCGAPWPTCPRSTSRR EGWLWTRTPSALGW*C*RPWLVRGL*RRTVPGPS I*KTWWKRRLRRLEWL*EAPRAHCKQVWLQMPG LLPSPCRSTRSTWAWASWPAAACTAGPKGGLL* PRCTRG*RSCRQWWRGCPGIRRPPAASPLPRRRT PTCPALAEPTVGLLHGSPWQRHQEPVPRQQSSC REAPTSPWRVTRA*AASLLPCAPGT*LQAALWT QHPSGRPAVLRGTRQENRAGGVAQDPGPQPWKDW PLAALHHRRQSHRRLSSTLPDRRWSRSWPCTRM GPWTACSCCRPAPSQAWAWNRTGRGPKKVMNFR ADVFTWADPPNPEVKVLMVRSSHGARVLSTLPAV GVGAHARGGEKEVALLF*ALWA*AGRVEPCLHA SIWGQGRLASSSEEAGACWEAAGCTDP*GEERG CCAGVWSRELAPLRAMQGVCSPGLWQQLFAHLFG QWPPCTMGPTRPRALLPAYNLEKCGRVRWLTPV IPALWEAKAGGSLEPSRSRPARAT**DPVSAKK FFKLLAWRGSARLWSQLLGRLK*EDHLCLGGRGC SES*LYDCTPAWVTEQDPVSKRKTLGKVKYGCK SHGSVLARSENSEILQKVEQHPPPTSGQCLQAL LGTCELA*CGGLQARPSLGATDELRARSGFGGHK LSLRPRH*LWQRGHYPRSS*AQDLVTQTVRSPW KAEKLGAWQTGKGNIFREKTCITCLQKQVRFHV TECPLACPKVAQGCSTGFTVILCSAVSHTTCPRE AGHW*RPGCP*VIKTYVAISGSTCGLCCTESLE LS*CLTLQGS*AS

Frame 1

SSPKHCGLCWLLCSERLLLPGVRLPAQRLPGGP SPLPDPGLPTSLLASATGHPSGYSPGNSVSTSG QPQPHPWRHQEFQRPSG*EADTQAGRLWPGPVQP LCRVQPQPEQHGGPDTDSAGHPGLPARGVHQDG KAGCGHGHLQLWGGSARDLGWSEGCEDARCQDQ VSERPGGRGG*GGWSGFEKHPEHTASRSGCRCLG CSHRHADLQEAPGPGPAGLLLPAPPGQKEASYD PGVREAREAAGSGGGGARAFGGRQLHPPFPAGE LLRVQHWQSPQWGCSMAAPGSAIRSQCPGSRAAA ERPQPARGE*REPRRPLCCPALLALDSKLPSGP STPQGGRLSSGGHGRRIELGEWPRIPAHSRGRT GPWQLCIIVVRATADYHQPCPTEDGPEAGPVRGW GPGQPAAAVVQLPPRLGPGTGQAGARRK**ISE LMCSPGQIPQIRKSKFSWSEVLMVHESSALCRQ WGWGPMPAGERRRWPCCSRLCGHRQAEWNPASMP ASGGKEGWHHPVRRLAHVGRLLAAQTREGRRGA AVQGCGVGSWLP*EPCRASAAQASGSSSLPISL DSGHPAQWGRRGLGPSYLLTIWKSVAGCGGSRL* SQHFGRPRQEDRWSPVGQDQPGQHDETLSLPKN FLNY*PGVVAHACGPSCWGG*SRRIIYAWEVEA AVSHDCMTALQPG*QSKTLFQKEKPWEK*SMAVS LMVQS*QEARILRSSRKSSSTHLQPRASVFRLY WGPASWPNVVACKPGHPWAPQTSSEPGQASEAT SSASGPGTDCGRGATTQGLARPKT*LPRQ*EAPG RQKSWEHGRQGRETFSGKRHVSHVFRSKSGFM* PSVLLRVQK*PRAVAQASQ*FCVQP*VTLHAPV KLGIGDVQVVLE**KRMLQSRALLVDFVAPKALS FPDALHFRVLER

Frame 2

RHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDR LHCQTQACPPLSWPQRLDILLGTARAIQFLHQD SPSLIHGDIKSSNVLLDERLTPKLGDFGLARFS RFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTG RLAVDTDTFSFGVVVLETLAGQRAVKTHGARTK YLKDLVEEEAEEAGVALRSTQSTLQAGLAADAW AAPIAMQIYKKHLGLGQLACCCLHRRAKRRPPMT QVYERLEKLQAVVAGVPGHSEAASCIPPSPQEN SYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQL QRGPNQPVESDESLGGLSAALRSWHLTPSCPLDP APLREAGCPQGDTAGESSWGSGPGSRPTAVEGL ALGSSASSSSEPPQIIINPARQKMVQKLALYED GALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS *CVHLGRSPKSGSQSSHGQKFSWCTSPQHSAGS GGGGPCPRGREGGGPAVLGSVGIGRQSGTLPPC QHLGARKAGIIQ*GGWRMLGGCWLHRPVRGGEGL LCRGVE*GAGSPESHAGRLQPRPLAAALCPSLW TVATLHNGADEA*GPPTCLQFGKVWPGAVAHAC NPSTLGGQGRRIAGAQ*VKTSQGNMMRPCLCQKI F*TISLAW*RTPVVPAAGEAEVGGSFMLGRSRL Q*VMIV*LHSSLGDRARPCFKKKNPGKSEVWL* VSWFSPSKKREF*DPPESRAAPTSNLGPVSSGFT GDLRAGLMWWPASQAIPGRHRRAPSQVRLRRPQ AQPQAQALIVAEGPLPKV*LGPRPSYPDSEKPL EGRKVGSMADREGKHFQGKDMYHMSSEASQVSCN RVSSCVSKSSPGL*HRLHSDFVFSRESHYMPP* SWALVTSRLSLSNKNVCCNLGLYLWTLLHRKP* AFLMPYTSGFLSV

Sequence IRAK1.fAug05

TTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCG TCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATCAGGAC AGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCC GCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAGACGGG AAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAG TATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGG CTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGT GTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTCGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTAC GTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAG GCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACC CCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTT GGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCC TGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGATG TGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAAGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGCAGTGGGGGT GGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGGAACCCTGCCTCCATGCCAGCATC TGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATGTTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGGGCTGCTGTG CAGGGGTGTGGAGTAGGGAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATCTCTTTGGACAGTG GCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTTGGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCA GCACTTTGGGAGGCCAAGGCAGGAGGATCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAAAATTTTTTA AACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGATCATTTATGCTTGGGAGGTCGAGGCTGCAGTGA GTCATGATTGTATGACTGCACTCCAGCCTGGGTGACAGAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGTAAGTCTCAT GGTTCAGTCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCAGTGTCTTCAGGCTTTACTGGGGA CCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTGGGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCACAAGCTCAG CCTCAGGCCCAGGCACTGATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAGCCCCTGGAAGGCA GAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGTATCACATGTCTTCAGAAGCAAGTCAGGTTTCATGTAACCGAGT GTCCTCTTGCGTGTCCAAAAGTAGCCCAGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCGTGAAGCTGG GCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAACGTATGTTGCAATCTCGGGCTCTACTTGTGGACTTTGTTGCACCGAAAGCCTTGAGCTTTCC TGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

FVTQTLWTLLATVLRTASTAWCTASCPTAPWRT VSTARPRPAHLSPGLSDWTSFWVQPGQFSFYIRT APASSMETSRVPTSFWMRG*HPSWETLAWPGSA ALPGPAPARAAWWPGHRQCGAPWPTCPRSTSRR EGWLWTRTPSALGW*C*RPWLVRGL*RRTVPGPS I*KTWWKRRLRRLEWL*EAPRAHCKQVWLQMPG LLPSPCRSTRSTWASWPAAACTAGPKGGLL*PR CTRG*RSCRQWWRGCPGIRRPPAASPLPRRRTPT CPALAEPTVGLLHGSPWQRHQEPVPRQQSSCRE APTSPWRVTRA*AASLLPCAPGT*LQAALWTQH PSGRPAVLRGTRQENRAGGVAQDPGPQPWKDWPL AALHHRRQSHRRLSSTLPDRRWSRSWPCTRMGP WTACSCCRPAPSQAWAWNRTGRGPKKVMNFRAD VFTWADPPNPEVKVLMVRSSHGARVLSTLPAVGV GAHARGGEKEVALLF*ALWA*AGRVEPCLHASI WGQGRLASSSEEAGACWEAAGCTDP*GEERGCC AGVWSRELAPLRAMQGVCSPGLWQQLFAHLFGQW PPCTMGPTRPRALLPAYNLEKCGRVRWLTPVIP ALWEAKAGGSLEPSRSRPARAT**DPVSAKKFF KLLAWRGSARLWSQLLGRLK*EDHLCLGGRGCSE S*LYDCTPAWVTEQDPVSKRKTLGKVKYGCKSH GSVLARSENSEILQKVEQHPPPTSGQCLQALLG TCELA*CGGLQARPSLGATDELRARSGFGGHKLS LRPRH*LWQRGHYPRSS*AQDLVTQTVRSPWKA EKLGAWQTGKGNIFREKTCITCLQKQVRFHVTE CPLACPKVAQGCSTGFTVILCSAVSHTTCPREAG HW*RPGCP*VIKTYVAISGSTCGLCCTESLELS *CLTLQGS*AS

Frame 1

SSPKHCGLCWLLCSERLLLPGVRLPAQRLPGGP SPLPDPGLPTSLLASATGHPSGYSPGNSVSTSG QPQPHPWRHQEFQRPSG*EADTQAGRLWPGPVQP LCRVQPQPEQHGGPDTDSAGHPGLPARGVHQDG KAGCGHGHLQLWGGSARDLGWSEGCEDARCQDQ VSERPGGRGG*GGWSGFEKHPEHTASRSGCRCLG CSHRHADLQEAPGPAGLLLPAPPGQKEASYDPG VREAREAAGSGGGGARAFGGRQLHPPFPAGELL RVQHWQSPQWGCSMAAPGSAIRSQCPGSRAAAER PQPARGE*REPRRPLCCPALLALDSKLPSGPST PQGGRLSSGGHGRRIELGEWPRIPAHSRGRTGP WQLCIIVVRATADYHQPCPTEDGPEAGPVRGWGP GQPAAAVVQLPPRLGPGTGQAGARRK**ISELM CSPGQIPQIRKSKFSWSEVLMVHESSALCRQWG WGPMPAGERRRWPCCSRLCGHRQAEWNPASMPAS GGKEGWHHPVRRLAHVGRLLAAQTREGRRGAAV QGCGVGSWLP*EPCRASAAQASGSSSLPISLDS GHPAQWGRRGLGPSYLLTIWKSVAGCGGSRL*SQ HFGRPRQEDRWSPVGQDQPGQHDETLSLPKNFL NY*PGVVAHACGPSCWGG*SRRIIYAWEVEAAV SHDCMTALQPG*QSKTLFQKEKPWEK*SMAVSLM VQS*QEARILRSSRKSSSTHLQPRASVFRLYWG PASWPNVVACKPGHPWAPQTSSEPGQASEATSS ASGPGTDCGRGATTQGLARPKT*LPRQ*EAPGRQ KSWEHGRQGRETFSGKRHVSHVFRSKSGFM*PS VLLRVQK*PRAVAQASQ*FCVQP*VTLHAPVKL GIGDVQVVLE**KRMLQSRALLVDFVAPKALSFP DALHFRVLER

Frame 2

RHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDR LHCQTQACPPLSWPQRLDILLGTARAIQFLHQD SPSLIHGDIKSSNVLLDERLTPKLGDFGLARFS RFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTG RLAVDTDTFSFGVVVLETLAGQRAVKTHGARTK YLKDLVEEEAEEAGVALRSTQSTLQAGLAADAW AAPIAMQIYKKHLGQLACCCLHRRAKRRPPMTQV YERLEKLQAVVAGVPGHSEAASCIPPSPQENSY VSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQR GPNQPVESDESLGGLSAALRSWHLTPSCPLDPAP LREAGCPQGDTAGESSWGSGPGSRPTAVEGLAL GSSASSSSEPPQIIINPARQKMVQKLALYEDGA LDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS*C VHLGRSPKSGSQSSHGQKFSWCTSPQHSAGSGG GGPCPRGREGGGPAVLGSVGIGRQSGTLPPCQH LGARKAGIIQ*GGWRMLGGCWLHRPVRGGEGLLC RGVE*GAGSPESHAGRLQPRPLAAALCPSLWTV ATLHNGADEA*GPPTCLQFGKVWPGAVAHACNP STLGGQGRRIAGAQ*VKTSQGNMMRPCLCQKIF* TISLAW*RTPVVPAAGEAEVGGSFMLGRSRLQ* VMIV*LHSSLGDRARPCFKKKNPGKSEVWL*VS WFSPSKKREF*DPPESRAAPTSNLGPVSSGFTGD LRAGLMWWPASQAIPGRHRRAPSQVRLRRPQAQ PQAQALIVAEGPLPKV*LGPRPSYPDSEKPLEG RKVGSMADREGKHFQGKDMYHMSSEASQVSCNRV SSCVSKSSPGL*HRLHSDFVFSRESHYMPP*SW ALVTSRLSLSNKNVCCNLGLYLWTLLHRKP*AF LMPYTSGFLSV

Sequence IRAK1.eAug05

TCGAGACAAGGAGGAAGGAATTCAGCCTTTGATGTAGCGCAGAGCCCCAGTCAGCCAAGCTGGGTCAGCTGGGAGGCAGCTGTGGTGGGGAGAGCCTGGA GCCTTGGGCAGAAGGGAAGAGACAGGGACCCCACCTGATCCAGGCTCTCTTCCCACAGCCAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGC CCCTTTCCGTTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGAGGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACC GGGCGGTGATGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAACGCTGACCTGGAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGA GCAGCTGTCCAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGC TCCCTGGAGGACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGT TTCTACATCAGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCT GGCCCGGTTCAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAG TACATCAAGACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACG GTGCCAGGACCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGC TGCAGATGCCTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCT CCTATGACCCAGGTGTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTCGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGC AGGAGAACTCCTACGTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGA GCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCT CTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGG AAGGACTGGCCCTTGGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTA CGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAA TTTCAGAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAAGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTG CCGGCAGTGGGGGTGGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCTTCACAGTGATTTTGT GTTCAGCCGTGAGTCACACTACATGCCCCCGTGAAGCTGGGCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAACGTATGTTGCAATCTCGGGCTC TACTTGTGGACTTTGTTGCACCGAAAGCCTTGAGCTTTCCTGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

SRQGGRNSAFDVAQSPSQPSWVSWEAAVVGRAW SLGQKGRDRDPT*SRLSSHSQAQRAQCPSCREPA PFRFAGPSVRFPGAPTTSRRSSRSGRVALGACT GR**GTRCML*RG*RRTLTWSGLQ*SRAS*PRW SSCPGFVTQTLWTLLATVLRTASTAWCTASCPTA PWRTVSTARPRPAHLSPGLSDWTSFWVQPGQFS FYIRTAPASSMETSRVPTSFWMRG*HPSWETLA WPGSAALPGPAPARAAWWPGHRQCGAPWPTCPRS TSRREGWLWTRTPSALGW*C*RPWLVRGL*RRT VPGPSI*KTWWKRRLRRLEWL*EAPRAHCKQVW LQMPGLLPSPCRSTRSAWASWPAAACTAGPKGGL L*PRCTRG*RSCRQWWRGCPGIRRPPAASPLPR RRTPTCPALAEPTVGLLHGSPWQRHQEPVPRQQ SSCREAPTSPWRVTRA*AASLLPCAPGT*LQAAL WTQHPSGRPAVLRGTRQENRAGGVAQDPGPQPW KDWPLAALHHRRQSHRRLSSTLPDRRWSRSWPC TRMGPWTACSCCRPAPSQAWAWNRTGRGPKKVMN FRADVFTWADPPNPEVKVLMVRSSHGARVLSTL PAVGVGAHARGGEKEVALLF*ALWA*AGFTVIL CSAVSHTTCPREAGHW*RPGCP*VIKTYVAISGS TCGLCCTESLELS*CLTLQGS*AS

Frame 1

RDKEEGIQPLM*RRAPVSQAGSAGRQLWWGEPG ALGRREETGTPPDPGSLPTARPRELSVPPAGSP PLSVLLAPL*DFPGHPQLLGGAQDRGGWLWVRVP GGDEEHGVCCEEAEGER*PGVDCSEAELPDRGG AAVQVSSPKHCGLCWLLCSERLLLPGVRLPAQR LPGGPSPLPDPGLPTSLLASATGHPSGYSPGNSV STSGQPQPHPWRHQEFQRPSG*EADTQAGRLWP GPVQPLCRVQPQPEQHGGPDTDSAGHPGLPARG VHQDGKAGCGHGHLQLWGGSARDLGWSEGCEDAR CQDQVSERPGGRGG*GGWSGFEKHPEHTASRSG CRCLGCSHRHADLQEAPGPAGLLLPAPPGQKEA SYDPGVREAREAAGSGGGGARAFGGRQLHPPFPA GELLRVQHWQSPQWGCSMAAPGSAIRSQCPGSR AAAERPQPARGE*REPRRPLCCPALLALDSKLP SGPSTPQGGRLSSGGHGRRIELGEWPRIPAHSRG RTGPWQLCIIVVRATADYHQPCPTEDGPEAGPV RGWGPGQPAAAVVQLPPRLGPGTGQAGARRK** ISELMCSPGQIPQIRKSKFSWSEVLMVHESSALC RQWGWGPMPAGERRRWPCCSRLCGHRQASQ*FC VQP*VTLHAPVKLGIGDVQVVLE**KRMLQSRA LLVDFVAPKALSFPDALHFRVLER

Frame 2

ETRRKEFSL*CSAEPQSAKLGQLGGSCGGESLE PWAEGKRQGPHLIQALFPQPGPESSVSLLQGAR PFPFCWPLCEISRGTHNFSEELKIGEGGFGCVY RAVMRNTVYAVKRLKENADLEWTAVKQSFLTEVE QLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNG SLEDRLHCQTQACPPLSWPQRLDILLGTARAIQ FLHQDSPSLIHGDIKSSNVLLDERLTPKLGDFGL ARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEE YIKTGRLAVDTDTFSFGVVVLETLAGQRAVKTH GARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLA ADAWAAPIAMQIYKKRLGQLACCCLHRRAKRRP PMTQVYERLEKLQAVVAGVPGHSEAASCIPPSP QENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAE QLQRGPNQPVESDESLGGLSAALRSWHLTPSCP LDPAPLREAGCPQGDTAGESSWGSGPGSRPTAV EGLALGSSASSSSEPPQIIINPARQKMVQKLALY EDGALDSLQLLSSSSLPGLGLEQDRQGPEESDE FQS*CVHLGRSPKSGSQSSHGQKFSWCTSPQHS AGSGGGGPCPRGREGGGPAVLGSVGIGRLHSDFV FSRESHYMPP*SWALVTSRLSLSNKNVCCNLGL YLWTLLHRKP*AFLMPYTSGFLSV

Sequence IRAK1.aAug05

GCGCAAGATGGCGGCGGGCCCGGGCACCGCCCCTTCCGCCCCGCCGGGCGTCGCACGAGGCCGGCTCGAAGGGGAAGTGAGTCAGTGTCCGCGGACCCGG CCGGCCCAGGCCCGCGCCCGCCGCGGCCCTGAGAGGCCCCGGCAGGTCCCGGCCCGGCGGCGGCAGCCATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGC AGCCCCCGGCGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAG TTCGCCGCCCTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACG CCCGTGTGGCCGACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCC AGGCACCACTGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCCTCAGCCTCCACCTTCCTCTCC CCAGCTTTTCCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTT CTACCAAGCCAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTTTCCGTTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAA CTTCTCGGAGGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAAC GCTGACCTGGAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACT GTGCTCAGAACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTC CTGGCCTCAGCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCC AACGTCCTTCTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGG TGGCCCGGACACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGG GGTGGTAGTGCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAG GCTGGAGTGGCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACC TGGACCCCAGGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTAT GACCCAGGTGTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTCGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAG AACTCCTACGTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGC TGCAGAGAGGCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGA CCCAGCACCCCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGA CTGGCCCTTGGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGG ATGGGGCCCTGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCA GAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAAAGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGC AGTGGGGGTGGGGGCCCATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGGAACCCTGCCTCCAT GCCAGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATGTTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGG GCTGCTGTGCAGGGGTGTGGAGTAGGGAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATCTCTT TGGACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTTGGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCT GTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGATCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAA AATTTTTTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGATCATTTATGCTTGGGAGGTCGAGG CTGCAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACAGAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGT AAGTCTCATGGTTCAGTCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCAGTGTCTTCAGGCTT TACTGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTGGGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCA CAAGCTCAGCCTCAGGCCCAGGCACTGATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAGCCCC TGGAAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGTATCACATGTCTTCAGAAGCAAGTCAGGTTTCATG TAACCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCCAGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCG TGAAGCTGGGCATTGGTGACGTCCAGGTTGTCCTTGAGTAATAAAAACGTATGTTGCAATCTCGGGCTCTACTTGTGGACTTTGTTGCACCGAAAGCCTT GAGCTTTCCTGATGCCTTACACTTCAGGGTTCTTGAGCGTCC

IRAK1.F9 (forward primer)
IRAK1.R2 (reverse primer)
Alternative acceptor

Frame 0

AQDGGGPGHRPFRPAGRRTRPARRGSESVSADP AGPGPRPPRP*EAPAGPGPAAAAMAGGPGPGEPA APGAQHFLYEVPPWVMCRFYKVMDALEPADWCQ FAALIVRDQTELRLCERSGQRTASVLWPWINRN ARVADLVHILTHLQLLRARDIITAWHPPAPLPSP GTTAPRPSSIPAPAEAEAWSPRKLPSSASTFLS PAFPGSQTHSGPELGLVPSPASLWPPPPSPAPS STKPGPESSVSLLQGARPFPFCWPLCEISRGTHN FSEELKIGEGGFGCVYRAVMRNTVYAVKRLKEN ADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGY CAQNGFYCLVYGFLPNGSLEDRLHCQTQACPPLS WPQRLDILLGTARAIQFLHQDSPSLIHGDIKSS NVLLDERLTPKLGDFGLARFSRFAGSSPSQSSM VARTQTVRGTLAYLPEEYIKTGRLAVDTDTFSFG VVVLETLAGQRAVKTHGARTKYLKDLVEEEAEE AGVALRSTQSTLQAGLAADAWAAPIAMQIYKKH LDPRPGPCPPELGLGLGQLACCCLHRRAKRRPPM TQVYERLEKLQAVVAGVPGHSEAASCIPPSPQE NSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQ LQRGPNQPVESDESLGGLSAALRSWHLTPSCPLD PAPLREAGCPQGDTAGESSWGSGPGSRPTAVEG LALGSSASSSSEPPQIIINPARQKMVQKLALYE DGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQ S*CVHLGRSPKSGSQSSHGQKFSWCTSPQHSAG SGGGGPCPRGREGGGPAVLGSVGIGRQSGTLPP CQHLGARKAGIIQ*GGWRMLGGCWLHRPVRGGEG LLCRGVE*GAGSPESHAGRLQPRPLAAALCPSL WTVATLHNGADEA*GPPTCLQFGKVWPGAVAHA CNPSTLGGQGRRIAGAQ*VKTSQGNMMRPCLCQK IF*TISLAW*RTPVVPAAGEAEVGGSFMLGRSR LQ*VMIV*LHSSLGDRARPCFKKKNPGKSEVWL *VSWFSPSKKREF*DPPESRAAPTSNLGPVSSGF TGDLRAGLMWWPASQAIPGRHRRAPSQVRLRRP QAQPQAQALIVAEGPLPKV*LGPRPSYPDSEKP LEGRKVGSMADREGKHFQGKDMYHMSSEASQVSC NRVSSCVSKSSPGL*HRLHSDFVFSRESHYMPP *SWALVTSRLSLSNKNVCCNLGLYLWTLLHRKP *AFLMPYTSGFLSV

Frame 1

RKMAAGPGTAPSAPPGVARGRLEGEVSQCPRTR PAQARARRGPERPRQVPARRRQPWPGGRARGSP QPPAPSTSCTRCRPGSCAASTK*WTPWSPPTGAS SPP*SCATRPSCGCASAPGSARPASCGPGSTAT PVWPTSCTSSRTCSCSVRGTSSQPGTLPPRFRP QAPLPRGPAASLHPPRPRPGAPGSCHPQPPPSSP QLFQAPRPIQGLSSAWSQALLPCGLHRHLQPLL LPSQAQRAQCPSCREPAPFRFAGPSVRFPGAPT TSRRSSRSGRVALGACTGR**GTRCML*RG*RRT LTWSGLQ*SRAS*PRWSSCPGFVTQTLWTLLAT VLRTASTAWCTASCPTAPWRTVSTARPRPAHLS PGLSDWTSFWVQPGQFSFYIRTAPASSMETSRVP TSFWMRG*HPSWETLAWPGSAALPGPAPARAAW WPGHRQCGAPWPTCPRSTSRREGWLWTRTPSAL GW*C*RPWLVRGL*RRTVPGPSI*KTWWKRRLRR LEWL*EAPRAHCKQVWLQMPGLLPSPCRSTRST WTPGPGPAHLSWAWAWASWPAAACTAGPKGGLL *PRCTRG*RSCRQWWRGCPGIRRPPAASPLPRRR TPTCPALAEPTVGLLHGSPWQRHQEPVPRQQSS CREAPTSPWRVTRA*AASLLPCAPGT*LQAALW TQHPSGRPAVLRGTRQENRAGGVAQDPGPQPWKD WPLAALHHRRQSHRRLSSTLPDRRWSRSWPCTR MGPWTACSCCRPAPSQAWAWNRTGRGPKKVMNF RADVFTWADPPNPEVKVLMVRSSHGARVLSTLPA VGVGAHARGGEKEVALLF*ALWA*AGRVEPCLH ASIWGQGRLASSSEEAGACWEAAGCTDP*GEER GCCAGVWSRELAPLRAMQGVCSPGLWQQLFAHLF GQWPPCTMGPTRPRALLPAYNLEKCGRVRWLTP VIPALWEAKAGGSLEPSRSRPARAT**DPVSAK KFFKLLAWRGSARLWSQLLGRLK*EDHLCLGGRG CSES*LYDCTPAWVTEQDPVSKRKTLGKVKYGC KSHGSVLARSENSEILQKVEQHPPPTSGQCLQA LLGTCELA*CGGLQARPSLGATDELRARSGFGGH KLSLRPRH*LWQRGHYPRSS*AQDLVTQTVRSP WKAEKLGAWQTGKGNIFREKTCITCLQKQVRFH VTECPLACPKVAQGCSTGFTVILCSAVSHTTCPR EAGHW*RPGCP*VIKTYVAISGSTCGLCCTESL ELS*CLTLQGS*AS

Frame 2

ARWRRARAPPLPPRRASHEAGSKGK*VSVRGPG RPRPAPAAALRGPGRSRPGGGSHGRGAGPGGAR SPRRPALLVRGAALGHVPLLQSDGRPGARRLVP VRRPDRARPDRAAAVRALRAAHGQRPVALDQPQR PCGRPRAHPHAPAAAPCAGHHHSLAPSRPASVP RHHCPEAQQHPCTRRGRGLEPPEVAILSLHLPL PSFSRLPDPFRA*ARPGPKPCFPVASTAISSPFF YQARPRELSVPPAGSPPLSVLLAPL*DFPGHPQ LLGGAQDRGGWLWVRVPGGDEEHGVCCEEAEGE R*PGVDCSEAELPDRGGAAVQVSSPKHCGLCWLL CSERLLLPGVRLPAQRLPGGPSPLPDPGLPTSL LASATGHPSGYSPGNSVSTSGQPQPHPWRHQEF QRPSG*EADTQAGRLWPGPVQPLCRVQPQPEQHG GPDTDSAGHPGLPARGVHQDGKAGCGHGHLQLW GGSARDLGWSEGCEDARCQDQVSERPGGRGG*G GWSGFEKHPEHTASRSGCRCLGCSHRHADLQEAP GPQARALPT*AGPGPGPAGLLLPAPPGQKEASY DPGVREAREAAGSGGGGARAFGGRQLHPPFPAG ELLRVQHWQSPQWGCSMAAPGSAIRSQCPGSRAA AERPQPARGE*REPRRPLCCPALLALDSKLPSG PSTPQGGRLSSGGHGRRIELGEWPRIPAHSRGR TGPWQLCIIVVRATADYHQPCPTEDGPEAGPVRG WGPGQPAAAVVQLPPRLGPGTGQAGARRK**IS ELMCSPGQIPQIRKSKFSWSEVLMVHESSALCR QWGWGPMPAGERRRWPCCSRLCGHRQAEWNPASM PASGGKEGWHHPVRRLAHVGRLLAAQTREGRRG AAVQGCGVGSWLP*EPCRASAAQASGSSSLPIS LDSGHPAQWGRRGLGPSYLLTIWKSVAGCGGSRL *SQHFGRPRQEDRWSPVGQDQPGQHDETLSLPK NFLNY*PGVVAHACGPSCWGG*SRRIIYAWEVE AAVSHDCMTALQPG*QSKTLFQKEKPWEK*SMAV SLMVQS*QEARILRSSRKSSSTHLQPRASVFRL YWGPASWPNVVACKPGHPWAPQTSSEPGQASEA TSSASGPGTDCGRGATTQGLARPKT*LPRQ*EAP GRQKSWEHGRQGRETFSGKRHVSHVFRSKSGFM *PSVLLRVQK*PRAVAQASQ*FCVQP*VTLHAP VKLGIGDVQVVLE**KRMLQSRALLVDFVAPKAL SFPDALHFRVLER