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Sequences

Sequence BARD1.cAug05

CCTCTGGCGGCCCGCCGTCCCAGACGCGGGAAGAGCTTGGCCGGTTTCGAGTCGCTGGCCTGCAGCTTCCCTGTGGTTTCCCGAGGCTTCCTTGCTTCCC
GCTCTGCGAGGAGCCTTTCATCCGAAGGCGGGACGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGATCCGCTCCGGGAACGAGCCTCGTTC
CGCGCCCGCCATGGAACCGGATGGTCGCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTGCTGCGCTGCTCGCGTTGATTTGAA
AGAAGATAAACCTAGGAAAAGTTTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTT
GTGAGTAAAGCTTCAGTGCAAACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATGAATTTGTTTCCCCAAGTCCTCCTGCAGATG
TTTCTGAGAGGGCTAAAAAGGCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAATCAACCAAAAATGGAATTTAGAGGCAGAAAA
AGAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTTCTGTAGCCAACCATCTGTTATCTCCAGTCCTCAGATAAATGGTGAA
ATAGACTTACTAGCAAGTGGCTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCCAGACA
CTAA
  • BARD1.u.f.1 (forward primer)
  • BARD1.u.r.5 (reverse primer)
  • Single exon skipping

Frame 0

PLAARRPRRGKSLAGFESLACSFPVVSRGFLAS
RSARSLSSEGGTMPDNRQPRNRQPRIRSGNEPRS
APAMEPDGRGAWAHSRAALDRLEKLLRCSR*FE
RR*T*EKFV**CRKQEEFN*NVV*PSK*ESQIC
CE*SFSANPACNKKRCKCSARLI*ICFPKSSCRC
F*EG*KGFCKIWKKAKKENFS*NQPKMEFRGRK
RRW*I*LQRGI*AKAGILL*PTICYLQSSDKW*
NRLTSKWLLDRI*MFWKFN*SLFTIG*ANRVSRH
*

Frame 1

LWRPAVPDAGRAWPVSSRWPAASLWFPEASLLP
ALRGAFHPKAGRCRIIGSRGTGSRGSAPGTSLV
PRPPWNRMVAVPGPTVAPRSTAWRSCCAARVDLK
EDKPRKSLFNDAGNKKNSIKMWFSPRSKKVRYV
VSKASVQTQPAIKKDASAQQDSYEFVSPSPPAD
VSERAKKASARSGKKQKKKTLAEINQKWNLEAEK
EDGEFDSKEESKQKLVSFCSQPSVISSPQINGE
IDLLASGSLTESECFGSLTEVSLPLAEQIESPD
T

Frame 2

SGGPPSQTREELGRFRVAGLQLPCGFPRLPCFP
LCEEPFIRRRDDAG*SAAEEPAAEDPLRERASF
RARHGTGWSRCLGPQSRRARPPGEAAALLALI*
KKINLGKVCLMMQETRRIQLKCGLALEVRKSDML
*VKLQCKPSLQ*KKMQVLSKTHMNLFPQVLLQM
FLRGLKRLLQDLEKSKKRKL*LKSTKNGI*RQK
KKMVNLTPKRNLSKSWYPSVANHLLSPVLR*MVK
*TY*QVAP*QNLNVLEV*LKSLYHWLSK*SLQT
L

Sequence BARD1.eAug05

CCTCTGGCGGCCCGCCGTCCCAGACGCGGGAAGAGCTTGGCCGGTTTCGAGTCGCTGGCCTGCAGCTTCCCTGTGGTTTCCCGAGGCTTCCTTGCTTCCC
GCTCTGCGAGGAGCCTTTCATCCGAAGGCGGGACGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGATCCGCTCCGGGAACGAGCCTCGTTC
CGCGCCCGCCATGGAACCGGATGGTCGCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTGCTGCGCTGCTCGCGTTGTACTAAC
ATTCTGAGAGAGCCTGTGTGTTTAGGAGGATGTGAGCACATCTTCTGTAGATTTGAAAGAAGATAAACCTAGGAAAAGTTTGTTTAATGATGCAGGAAAC
AAGAAGAATTCAATTAAAATGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCAAACCCAGCCTGCAATAAAAAAAG
ATGCAAGTGCTCAGCAAGACTCATATGAATTTGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAGGCTTCTGCAAGATCTGGAAAAAA
GCAAAAAAAGAAAACTTTAGCTGAAATCAACCAAAAATGGAATTTAGAGGCAGAAAAAGAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAG
CTGGTATCCTTCTGTAGCCAACCATCTGTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTGACAGAATCTGAATGTT
TTGGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCCAGACACTAA
  • BARD1.u.f.1 (forward primer)
  • BARD1.u.r.5 (reverse primer)
  • Single exon skipping

Frame 0

PLAARRPRRGKSLAGFESLACSFPVVSRGFLAS
RSARSLSSEGGTMPDNRQPRNRQPRIRSGNEPRS
APAMEPDGRGAWAHSRAALDRLEKLLRCSRCTN
ILREPVCLGGCEHIFCRFERR*T*EKFV**CRK
QEEFN*NVV*PSK*ESQICCE*SFSANPACNKKR
CKCSARLI*ICFPKSSCRCF*EG*KGFCKIWKK
AKKENFS*NQPKMEFRGRKRRW*I*LQRGI*AK
AGILL*PTICYLQSSDKW*NRLTSKWLLDRI*MF
WKFN*SLFTIG*ANRVSRH*

Frame 1

LWRPAVPDAGRAWPVSSRWPAASLWFPEASLLP
ALRGAFHPKAGRCRIIGSRGTGSRGSAPGTSLV
PRPPWNRMVAVPGPTVAPRSTAWRSCCAARVVLT
F*ESLCV*EDVSTSSVDLKEDKPRKSLFNDAGN
KKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKK
DASAQQDSYEFVSPSPPADVSERAKKASARSGKK
QKKKTLAEINQKWNLEAEKEDGEFDSKEESKQK
LVSFCSQPSVISSPQINGEIDLLASGSLTESEC
FGSLTEVSLPLAEQIESPDT

Frame 2

SGGPPSQTREELGRFRVAGLQLPCGFPRLPCFP
LCEEPFIRRRDDAG*SAAEEPAAEDPLRERASF
RARHGTGWSRCLGPQSRRARPPGEAAALLALY*
HSERACVFRRM*AHLL*I*KKINLGKVCLMMQET
RRIQLKCGLALEVRKSDML*VKLQCKPSLQ*KK
MQVLSKTHMNLFPQVLLQMFLRGLKRLLQDLEK
SKKRKL*LKSTKNGI*RQKKKMVNLTPKRNLSKS
WYPSVANHLLSPVLR*MVK*TY*QVAP*QNLNV
LEV*LKSLYHWLSK*SLQTL

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