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Sequences

Sequence BARD1.eAug05

BARD1.u.f.1 (forward primer)
BARD1.u.r.5 (reverse primer)
Single exon skipping

Frame 0

PLAARRPRRGKSLAGFESLACSFPVVSRGFLAS RSARSLSSEGGTMPDNRQPRNRQPRIRSGNEPRS APAMEPDGRGAWAHSRAALDRLEKLLRCSRCTN ILREPVCLGGCEHIFCRFERR*T*EKFV**CRK QEEFN*NVV*PSK*ESQICCE*SFSANPACNKKR CKCSARLI*ICFPKSSCRCF*EG*KGFCKIWKK AKKENFS*NQPKMEFRGRKRRW*I*LQRGI*AK AGILL*PTICYLQSSDKW*NRLTSKWLLDRI*MF WKFN*SLFTIG*ANRVSRH*

Frame 1

LWRPAVPDAGRAWPVSSRWPAASLWFPEASLLP ALRGAFHPKAGRCRIIGSRGTGSRGSAPGTSLV PRPPWNRMVAVPGPTVAPRSTAWRSCCAARVVLT F*ESLCV*EDVSTSSVDLKEDKPRKSLFNDAGN KKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKK DASAQQDSYEFVSPSPPADVSERAKKASARSGKK QKKKTLAEINQKWNLEAEKEDGEFDSKEESKQK LVSFCSQPSVISSPQINGEIDLLASGSLTESEC FGSLTEVSLPLAEQIESPDT

Frame 2

SGGPPSQTREELGRFRVAGLQLPCGFPRLPCFP LCEEPFIRRRDDAG*SAAEEPAAEDPLRERASF RARHGTGWSRCLGPQSRRARPPGEAAALLALY* HSERACVFRRM*AHLL*I*KKINLGKVCLMMQET RRIQLKCGLALEVRKSDML*VKLQCKPSLQ*KK MQVLSKTHMNLFPQVLLQMFLRGLKRLLQDLEK SKKRKL*LKSTKNGI*RQKKKMVNLTPKRNLSKS WYPSVANHLLSPVLR*MVK*TY*QVAP*QNLNV LEV*LKSLYHWLSK*SLQTL

Sequence BARD1.aAug05

CCTCTGGCGGCCCGCCGTCCCAGACGCGGGAAGAGCTTGGCCGGTTTCGAGTCGCTGGCCTGCAGCTTCCCTGTGGTTTCCCGAGGCTTCCTTGCTTCCC GCTCTGCGAGGAGCCTTTCATCCGAAGGCGGGACGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGATCCGCTCCGGGAACGAGCCTCGTTC CGCGCCCGCCATGGAACCGGATGGTCGCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTGCTGCGCTGCTCGCGTTGTACTAAC ATTCTGAGAGAGCCTGTGTGTTTAGGAGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAACTGGATGTCCAGTGTGTTACACCC CGGCCTGGATACAAGACTTGAAGATAAATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTTGCTACATGACAATGAGCTGTCAGA TTTGAAAGAAGATAAACCTAGGAAAAGTTTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGA TATGTTGTGAGTAAAGCTTCAGTGCAAACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATGAATTTGTTTCCCCAAGTCCTCCTG CAGATGTTTCTGAGAGGGCTAAAAAGGCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAATCAACCAAAAATGGAATTTAGAGGC AGAAAAAGAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTTCTGTAGCCAACCATCTGTTATCTCCAGTCCTCAGATAAAT GGTGAAATAGACTTACTAGCAAGTGGCTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTC CAGACACTAAGAGCAGGAATGAAGTAGTGACTCCTGAGAAGGTCTGCAAAAATTATCTTACATCTAAGAAATCTTTGCCATTAGAAAATAATGGAAAACG TGGCCATCACAATAGACTTTCCAGTCCCATTTCTAAGAGATGTAGAACCAGCATTCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAA AATATACCATTGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGGAAAAACAGTAACATGTCCGATGAATTCATTA GTCTTTCACCAGGTACACCACCTTCTACATTAAGTAGTTCAAGTTACAGGCGAGTGATGTCTAGTCCCTCAGCAATGAAGCTGTTGCCCAATATGGCTGT GAAAAGAAATCATAGAGGAGAGACTTTGCTCCATATTGCTTCTATTAAGGGCGACATACCTTCTGTTGAATACCTTTTACAAAATGGAAGTGATCCAAAT GTTAAAGACCATGCTGGATGGACACCATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGCTCCAGCATAAGGCATTGGTGAACA CCACCGGGTATCAAAATGACTCACCACTTCACGATGCAGCCAAGAATGGGCATGTGGATATAGTCAAGCTGTTACTTTCCTATGGAGCCTCCAGAAATGC TGTTAATATATTTGGTCTGCGGCCTGTCGATTATACAGATGATGAAAGTATGAAATCGCTATTGCTGCTACCAGAGAAGAATGAATCATCCTCAGCTAGC CACTGCTCAGTAATGAACACTGGGCAGCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAACAACAGAAAATGCTCAGTGAGCTTG CAGTAATTCTTAAGGCTAAAAAATATACTGAGTTTGACAGTACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGTTCAAAGTACCTTGAAGTGTATGCT TGGGATTCTCAATGGATGCTGGATTCTAAAATTTGAATGGGTAAAAGCATGTCTACGAAGAAAAGTATGTGAACAGGAAGAAAAGTATGAAATTCCTGAA GGTCCACGCAGAAGCAGGCTCAACAGAGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGGGAACCTTCAAACACCATCCAAAGG ACAACCTTATTAAGCTCGTCACTGCAGGTGGGGGCCAGATCCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGACTCAGACCATCAATACAGTCGCATA CCATGCGAGACCCGATTCTGATCAGCGCTTCTGCACACAGTATATCATCTATGAAGATTTGTGTAATTATCACCCAGAGAGGGTTCGGCAGGGCAAAGTC TGGAAGGCTCCTTCGAGCTGGTTTATAGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAATATTATACCAGATGAACATTTCAAATTGAA TTTGCACGGTTTGTGAGAGCCCAGTCATTGTACTGTTTTTAATGTTCACATTTTTACAAATAGGTAGAGTCATTCATATTTGTCTTTGAATCAAAAAAAA AAAAAAAGTCTAATGCCAGATTAGGAATTCATGTTATGTTTACCATTTAGAAGCTGGGATTGCTTTTAAAGGTTTTTCTTTTTAAAATTGGCATGTTTTT GATTTATCATGTCTTTCTATTCAGATTATTGGGTATCAAAGATTAATGAGGACACCAGAATCTTGGTTAAATAGACAAGTGGTATCATTACTGTTTGAGT CTTTTAATATTCTCCATACCTGCCACCAGTGAAAAAACTTGCCTTTTTTTTTTTTTTTTTTTTAGTAAACAGAATATTATCAAACAATTTATTTTGGCTT TATTGAAAAAAGAGTATTTGGTCTAAATGTGCCACCATAGGTGTTAAATTCTCCTATCTGCAATTGTCTTTATCCTATATTGTGTTCATTTCTTTTCTTA ATAATTTACTTTGTTGTGTGTTTCTACACTTTCATCCCTGTTTTTTATCTTGTATATCATCAGGAAATTGTGATTTAATCATTAACATTGGTTTTTTTGT GTGTGTGGTAAAAATCAACACTAGGCTCATGGTACATATTTTTATTCTGTACATTTGCTTGTAACTATCAATTTGTAACTCTGTTTATCTACTACATGTG TATATATACTTAGAGCATTTTCTCTAACACATTTTAATGTTAGTATTTTTTAAAAGGTCTGACCAGTCTAGCAAATTGTCAGTCCAACGTCATTACTTTA AATTAAGAAGCAGTCTTCTTCTGGTAAACCTTGTTGGTATTTGTAAAATAATTTTGAAGGTCTTAATTTCTTCCTTTGTAAAAGGAAAAGGTTTTTTTTA AAGTTTTTAGGTTGGCATGGAGGCAGAAGTTGGTGATTACTTGATTTACAACAGATTTTTTCCAGATCATACAAAAGGCCATACAGTAAGTATAGAAGTA GGTATGGGGAGGGCTTACTAATATCAAATAGGCAAGGCCTTAGTGAGTGGGCAGGATACCACCTGAGAGTGGCCAGATGTGGGGAGGTTACTCTGCTCTG GGTGCTCTCATTCATGAATCGACAAGGATACATTAGATTATTTTGAAACATTTTTTTAAGAAGCAGAATTCTTTAATAATTCCTTCCTAGACATTGAATA TACTTATAAAATTAAAGACTTGGGGAAGGAGACACTGAGAGACTTGCCAGTTTGGTTCCTCATGAACAAAAGAGGACAGTTTGATAACTACCAGAATAGA ATATCCCTAGTTTTAAAATAGTGAGAATCTCTGAAGTTCATCAACATCTTAAGATGCACTTACTTGAAAGTTTGAGATTCTGTTTATCATTTGAAAACAC ATTTTGCTTTAATTCTTTCTTTGACATGTTGTTTTTTCATATCAAGAAATATATGAACAAAATAATAACCTTTTGACCCTGACCTTGCTGGGTGAATTAG CTCTGAAACACTCTCTACAACCAGTAATGCATTTGTCCCACATTTCATTCTGATAGAAAATGAACACCATAGCACCAAACAAAAATCCGAGGCGTTAGAT AATGTCTGGATTAAATAATTTAAGACTCTCTAGGATTTTGGTTGTCATTTTTTATTTATAACAGACTTTAAGTCACTTTCTGTTGCCTCATAGGTCACAT TTTAGACAGGTTTGTGTCTGTTCCTTGCATCTGAATTCCTGATTGTAAAGACACCTATGAGGTCTCTTAGTTTTTGTCATTCATTTTCTTGGTTTATCAC CCCTCCCTTCTTTTTGTTGTTTTTCCCTGACTGTTAAGCAGTTTCATCTTTGCTTTTGTTAAATATTTGACAGCAGTTAGTTTGTGTTAAGCTCTTGAAA CTTGTGATTGTACTTTCTGTGTAGATATACATGTAATTATTTTTTATTTTTCAATCATAGATTCAAGCTTCCTTCTTTTTTACCACAAATCATTAAAGTT ATTTGTGTTTCCATATACCTGTGTCTTGTATAAAATTGGCTTATTCTGTGCTGTTGAATGAGGCTCAACATGACTTGGTGAGGAAGTCTATTAACTAACA AAAGCTTATCTTTTTTAACATAATGCTTTTTAATTAATTTTGAATAAAAATATTTCTAAAGTGTACTAGATACTTTATTACCTTAGATTATTCCGAATAC AGTATAACTTTGATAGTTTGGAATAGTCATTAAGAAACAATTACACACTGATTGCTTTGTGTCTCTAAAAGTGAGAGGCTGGTAGCTTTTCCACATTCTC ATGGCTATTTT

BARD1.u.f.1 (forward primer)
BARD1.u.r.5 (reverse primer)
Single exon skipping

Frame 0

PLAARRPRRGKSLAGFESLACSFPVVSRGFLAS RSARSLSSEGGTMPDNRQPRNRQPRIRSGNEPRS APAMEPDGRGAWAHSRAALDRLEKLLRCSRCTN ILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYT PAWIQDLKINRQLDSMIQLCSKLRNLLHDNELSD LKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKVR YVVSKASVQTQPAIKKDASAQQDSYEFVSPSPP ADVSERAKKASARSGKKQKKKTLAEINQKWNLEA EKEDGEFDSKEESKQKLVSFCSQPSVISSPQIN GEIDLLASGSLTESECFGSLTEVSLPLAEQIES PDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKR GHHNRLSSPISKRCRTSILSTSGDFVKQTVPSE NIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFI SLSPGTPPSTLSSSSYRRVMSSPSAMKLLPNMAV KRNHRGETLLHIASIKGDIPSVEYLLQNGSDPN VKDHAGWTPLHEACNHGHLKVVELLLQHKALVN TTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNA VNIFGLRPVDYTDDESMKSLLLLPEKNESSSAS HCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSEL AVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCML GILNGCWILKFEWVKACLRRKVCEQEEKYEIPE GPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPK DNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAY HARPDSDQRFCTQYIIYEDLCNYHPERVRQGKV WKAPSSWFIDCVMSFELLPLDS*ILYQMNISN* ICTVCESPVIVLFLMFTFLQIGRVIHICL*IKKK KKV*CQIRNSCYVYHLEAGIAFKGFSF*NWHVF DLSCLSIQIIGYQRLMRTPESWLNRQVVSLLFE SFNILHTCHQ*KNLPFFFFFF**TEYYQTIYFGF IEKRVFGLNVPP*VLNSPICNCLYPILCSFLFL IIYFVVCFYTFIPVFYLVYHQEIVI*SLTLVFL CVW*KSTLGSWYIFLFCTFACNYQFVTLFIYYMC IYT*SIFSNTF*C*YFLKGLTSLANCQSNVITL N*EAVFFW*TLLVFVK*F*RS*FLPL*KEKVFF KVFRLAWRQKLVIT*FTTDFFQIIQKAIQ*V*K* VWGGLTNIK*ARP**VGRIPPESGQMWGGYSAL GALIHESTRIH*IILKHFFKKQNSLIIPS*TLN ILIKLKTWGRRH*ETCQFGSS*TKEDSLITTRIE YP*F*NSENL*SSSTS*DALT*KFEILFII*KH ILL*FFL*HVVFSYQEIYEQNNNLLTLTLLGEL ALKHSLQPVMHLSHISF**KMNTIAPNKNPRR*I MSGLNNLRLSRILVVIFYL*QTLSHFLLPHRSH FRQVCVCSLHLNS*L*RHL*GLLVFVIHFLGLS PLPSFCCFSLTVKQFHLCFC*IFDSS*FVLSS*N L*LYFLCRYTCNYFLFFNHRFKLPSFLPQIIKV ICVSIYLCLV*NWLILCC*MRLNMTW*GSLLTN KSLSFLT*CFLINFE*KYF*SVLDTLLP*IIPNT V*L**FGIVIKKQLHTDCFVSLKVRGW*LFHIL MAI

Frame 1

LWRPAVPDAGRAWPVSSRWPAASLWFPEASLLP ALRGAFHPKAGRCRIIGSRGTGSRGSAPGTSLV PRPPWNRMVAVPGPTVAPRSTAWRSCCAARVVLT F*ESLCV*EDVSTSSVVIV*VTALELDVQCVTP RPGYKT*R*IDNWTA*FNFVVSFEICYMTMSCQ I*KKINLGKVCLMMQETRRIQLKCGLALEVRKSD ML*VKLQCKPSLQ*KKMQVLSKTHMNLFPQVLL QMFLRGLKRLLQDLEKSKKRKL*LKSTKNGI*R QKKKMVNLTPKRNLSKSWYPSVANHLLSPVLR*M VK*TY*QVAP*QNLNVLEV*LKSLYHWLSK*SL QTLRAGMK**LLRRSAKIILHLRNLCH*KIMEN VAITIDFPVPFLRDVEPAF*APVEILLSKRCPQK IYHCLNVLHHLHANVKLVVHQGGKTVTCPMNSL VFHQVHHLLH*VVQVTGE*CLVPQQ*SCCPIWL *KEIIEERLCSILLLLRATYLLLNTFYKMEVIQM LKTMLDGHHCMKLAIMGT*R*WNYCSSIRHW*T PPGIKMTHHFTMQPRMGMWI*SSCYFPMEPPEM LLIYLVCGLSIIQMMKV*NRYCCYQRRMNHPQLA TAQ**TLGSVGMDLLYL*AVGCLQNNRKCSVSL Q*FLRLKNILSLTVQ*LMLLFLVMQFKVP*SVC LGFSMDAGF*NLNG*KHVYEEKYVNRKKSMKFLK VHAEAGSTENSCCQSCLMDATSICGEPSNTIQR TTLLSSSLQVGARSSVESPSQTVT*LRPSIQSH TMRDPILISASAHSISSMKICVIITQRGFGRAKS GRLLRAGL*TV*CPLSCFLLTAEYYTR*TFQIE FARFVRAQSLYCF*CSHFYK*VESFIFVFESKK KKKSNARLGIHVMFTI*KLGLLLKVFLFKIGMFL IYHVFLFRLLGIKD**GHQNLG*IDKWYHYCLS LLIFSIPATSEKTCLFFFFFFSKQNIIKQFILA LLKKEYLV*MCHHRC*ILLSAIVFILYCVHFFS* *FTLLCVSTLSSLFFILYIIRKL*FNH*HWFFC VCGKNQH*AHGTYFYSVHLLVTINL*LCLSTTC VYILRAFSLTHFNVSIF*KV*PV*QIVSPTSLL* IKKQSSSGKPCWYL*NNFEGLNFFLCKRKRFFL KFLGWHGGRSW*LLDLQQIFSRSYKRPYSKYRS RYGEGLLISNRQGLSEWAGYHLRVARCGEVTLLW VLSFMNRQGYIRLF*NIFLRSRIL**FLPRH*I YL*N*RLGEGDTERLASLVPHEQKRTV**LPE* NIPSFKIVRISEVHQHLKMHLLESLRFCLSFENT FCFNSFFDMLFFHIKKYMNKIITF*P*PCWVN* L*NTLYNQ*CICPTFHSDRK*TP*HQTKIRGVR *CLD*II*DSLGFWLSFFIYNRL*VTFCCLIGHI LDRFVSVPCI*IPDCKDTYEVS*FLSFIFLVYH PSLLFVVFP*LLSSFIFAFVKYLTAVSLC*ALE TCDCTFCVDIHVIIFYFSIIDSSFLLFYHKSLKL FVFPYTCVLYKIGLFCAVE*GST*LGEEVY*LT KAYLF*HNAF*LILNKNISKVY*ILYYLRLFRI QYNFDSLE*SLRNNYTLIALCL*K*EAGSFSTFS WLF

Frame 2

SGGPPSQTREELGRFRVAGLQLPCGFPRLPCFP LCEEPFIRRRDDAG*SAAEEPAAEDPLRERASF RARHGTGWSRCLGPQSRRARPPGEAAALLALY* HSERACVFRRM*AHLL**LCK*LHWNWMSSVLHP GLDTRLEDK*TTGQHDSTL**ASKFAT*Q*AVR FERR*T*EKFV**CRKQEEFN*NVV*PSK*ESQ ICCE*SFSANPACNKKRCKCSARLI*ICFPKSSC RCF*EG*KGFCKIWKKAKKENFS*NQPKMEFRG RKRRW*I*LQRGI*AKAGILL*PTICYLQSSDK W*NRLTSKWLLDRI*MFWKFN*SLFTIG*ANRVS RH*EQE*SSDS*EGLQKLSYI*EIFAIRK*WKT WPSQ*TFQSHF*EM*NQHSEHQWRFC*ANGALR KYTIA*MFFTTFMQT*SWWYIREEKQ*HVR*IH* SFTRYTTFYIK*FKLQASDV*SLSNEAVAQYGC EKKS*RRDFAPYCFY*GRHTFC*IPFTKWK*SK C*RPCWMDTIA*SLQSWAPEGSGIIAPA*GIGEH HRVSK*LTTSRCSQEWACGYSQAVTFLWSLQKC C*YIWSAACRLYR**KYEIAIAATREE*IILS* PLLSNEHWAA*GWTSCTYRQWAVFRTTENAQ*AC SNS*G*KIY*V*QYSNSCCCSW*CSSKYLEVYA WDSQWMLDSKI*MGKSMSTKKSM*TGRKV*NS* RSTQKQAQQRTAVAKAV*WMLLLFVGNLQTPSKG QPY*ARHCRWGPDPQ*KAQARQ*RDSDHQYSRI PCETRF*SALLHTVYHL*RFV*LSPREGSAGQS LEGSFELVYRLCDVL*VASS*QLNIIPDEHFKLN LHGL*EPSHCTVFNVHIFTNR*SHSYLSLNQKK KKSLMPD*EFMLCLPFRSWDCF*RFFFLKLACF *FIMSFYSDYWVSKINEDTRILVK*TSGIITV*V F*YSPYLPPVKKLAFFFFFFLVNRILSNNLFWL Y*KKSIWSKCATIGVKFSYLQLSLSYIVFISFL NNLLCCVFLHFHPCFLSCISSGNCDLIINIGFFV CVVKINTRLMVHIFILYICL*LSICNSVYLLHV YIYLEHFL*HILMLVFFKRSDQSSKLSVQRHYF KLRSSLLLVNLVGICKIILKVLISSFVKGKGFF* SF*VGMEAEVGDYLIYNRFFPDHTKGHTVSIEV GMGRAY*YQIGKALVSGQDTT*EWPDVGRLLCS GCSHS*IDKDTLDYFETFF*EAEFFNNSFLDIEY TYKIKDLGKETLRDLPVWFLMNKRGQFDNYQNR ISLVLK**ESLKFINILRCTYLKV*DSVYHLKT HFALILSLTCCFFISRNI*TK**PFDPDLAG*IS SETLSTTSNAFVPHFILIENEHHSTKQKSEALD NVWIK*FKTL*DFGCHFLFITDFKSLSVAS*VT F*TGLCLFLASEFLIVKTPMRSLSFCHSFSWFIT PPFFLLFFPDC*AVSSLLLLNI*QQLVCVKLLK LVIVLSV*IYM*LFFIFQS*IQASFFFTTNH*S YLCFHIPVSCIKLAYSVLLNEAQHDLVRKSIN*Q KLIFFNIMLFN*F*IKIFLKCTRYFITLDYSEY SITLIVWNSH*ETITH*LLCVSKSERLVAFPHS HGYF