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Sequences

Sequence PDCD10.lAug05

CGACTCCGGCTGGGCAGGATTCCGGACAACGCCTGGTTCCTCTTGGGTCCTTCCGGCGTCGCCGGAGTGAATTGATCCGGGAGTTGAAGAGGGCTGCAAG GTGGGAAGTGAAGTCAGTGCCTCAGTTGCTGAGGAGCACTGGAAGAAGGAAGAGACAAATGTTGGGGTCTACGGATTCAGTTCCAGTTTAATGATGTGGC CCTCTAAGGAGATAATTTTGCCTTAAACAGAAAGACTTGACCCTCTTAGGTGTCATACCTAAGATCAGTGTGTTTTTTGTGTCCAATTCTTTTATCACCA AAAAAGAGAAGAAATATTGCAGTGAATGAAGATTCCTCTGCATTTTAGCACTGCTTTTTCAACTGTAGTTGGCTTTTGAATGAGGATGACAATGGAAGAG ATGAAGAATGAAGCTGAGACCACATCCATGGTTTCTATGCCCCTCTATGCAGTCATGTATCCTGTGTTTAATGAGCTAGAACGAGTAAATCTGTCTGCAG CCCAGACACTGAGAGCCGCTTTCATCAAGGCTGAAAAAGAAAATCCAGGTCTCACACAAGACATCATTATGAAAATTTTAGAGAAAAAAAGCGTGGAAGT TAACTTCACGGAGTCCCTTCTTCGTATGGCAGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCCAAGACCTAAACGAAAAGGCA CGAGCACTTAAACAAATTCTCAGTAAGATCCCAGATGAGATCAATGACAGAGTGAGGTTTCTGCAGACAATCAA

PDCD10.u.f.21 (forward primer)
PDCD10.u.r.7 (reverse primer)
Single exon skipping

Frame 0

RLRLGRIPDNAWFLLGPSGVAGVN*SGS*RGLQ GGK*SQCLSC*GALEEGRDKCWGLRIQFQFNDVA L*GDNFALNRKT*PS*VSYLRSVCFLCPILLSP KKRRNIAVNEDSSAF*HCFFNCSWLLNEDDNGR DEE*S*DHIHGFYAPLCSHVSCV**ARTSKSVCS PDTESRFHQG*KRKSRSHTRHHYENFREKKRGS *LHGVPSSYGS**CRRVYD*TTRARIPRPKRKG TST*TNSQ*DPR*DQ*QSEVSADNQ

Frame 1

DSGWAGFRTTPGSSWVLPASPE*IDPGVEEGCK VGSEVSASVAEEHWKKEETNVGVYGFSSSLMMW PSKEIILP*TERLDPLRCHT*DQCVFCVQFFYHQ KREEILQ*MKIPLHFSTAFSTVVGF*MRMTMEE MKNEAETTSMVSMPLYAVMYPVFNELERVNLSA AQTLRAAFIKAEKENPGLTQDIIMKILEKKSVEV NFTESLLRMAADDVEEYMIERPEPEFQDLNEKA RALKQILSKIPDEINDRVRFLQTI

Frame 2

TPAGQDSGQRLVPLGSFRRRRSELIRELKRAAR WEVKSVPQLLRSTGRRKRQMLGSTDSVPV**CG PLRR*FCLKQKDLTLLGVIPKISVFFVSNSFIT KKEKKYCSE*RFLCILALLFQL*LAFE*G*QWKR *RMKLRPHPWFLCPSMQSCILCLMS*NE*ICLQ PRH*EPLSSRLKKKIQVSHKTSL*KF*RKKAWK LTSRSPFFVWQLMM*KSI*LNDQSQNSKT*TKRH EHLNKFSVRSQMRSMTE*GFCRQS

Sequence PDCD10.iAug05

CGACTCCGGCTGGGCAGGATTCCGGACAACGCCTGGTTCCTCTTGGGTCCTTCCGGCGTCGCCGGAGTGAATTGATCCGGGAGTTGAAGAGGGCTGCAAG GTGGGAAGTGAAGTCAGTGCCTCAGTTGCTGAAAGACTTGACCCTCTTAGGTGTCATACCTAAGATCAGTGTGTTTTTTGTGTCCAATTCTTTTATCACC AAAAAAGAGAAGAAATATTGCAGTGAATGAAGATTCCTCTGCATTTTAGCACTGCTTTTTCAACTGTAGTTGGCTTTTGAATGAGGATGACAATGGAAGA GATGAAGAATGAAGCTGAGACCACATCCATGGTTTCTATGCCCCTCTATGCAGTCATGTATCCTGTGTTTAATGAGCTAGAACGAGTAAATCTGTCTGCA GCCCAGACACTGAGAGCCGCTTTCATCAAGGCTGAAAAAGAAAATCCAGGTCTCACACAAGACATCATTATGAAAATTTTAGAGAAAAAAAGCGTGGAAG TTAACTTCACGGAGTCCCTTCTTCGTATGGCAGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCCAAGACCTAAACGAAAAGGC ACGAGCACTTAAACAAATTCTCAGTAAGATCCCAGATGAGATCAATGACAGAGTGAGGTTTCTGCAGACAATCAAGGATATAGCTAGTGCAATAAAAGAA CTTCTTGATACAGTGAATAATGTCTTCAAGAAATATCAATACCAGAACCGCAGGGTACGTTTAGTAGAAGCCTTGCTAGTTGACACCCTAGATGTTGGTT TATGCCTAGTTGTTTTATTGACTTAAAAATAAGATTGATTGTTGGAAATAGTTTTGTGTCATTAACCATTGAGTTTATTCTTGAATTTAAATATGCCTTT TTATTTAAAAGTGAGTACTCCACAATTGCTTATTAGTAATATTCATATAAAGTGCATCCCATATCCTGTGTAATCTCTTGTCCTTGCCTTTGGCTTTTGT GATTATTTCTCTTATTTAGTACTTTATTTCTTATTATATTT

PDCD10.u.f.21 (forward primer)
PDCD10.u.r.7 (reverse primer)
Single exon skipping

Frame 0

RLRLGRIPDNAWFLLGPSGVAGVN*SGS*RGLQ GGK*SQCLSC*KT*PS*VSYLRSVCFLCPILLSP KKRRNIAVNEDSSAF*HCFFNCSWLLNEDDNGR DEE*S*DHIHGFYAPLCSHVSCV**ARTSKSVC SPDTESRFHQG*KRKSRSHTRHHYENFREKKRGS *LHGVPSSYGS**CRRVYD*TTRARIPRPKRKG TST*TNSQ*DPR*DQ*QSEVSADNQGYS*CNKR TS*YSE*CLQEISIPEPQGTFSRSLAS*HPRCWF MPSCFIDLKIRLIVGNSFVSLTIEFILEFKYAF LFKSEYSTIAY**YSYKVHPISCVISCPCLWLL *LFLLFSTLFLIIF

Frame 1

DSGWAGFRTTPGSSWVLPASPE*IDPGVEEGCK VGSEVSASVAERLDPLRCHT*DQCVFCVQFFYH QKREEILQ*MKIPLHFSTAFSTVVGF*MRMTMEE MKNEAETTSMVSMPLYAVMYPVFNELERVNLSA AQTLRAAFIKAEKENPGLTQDIIMKILEKKSVE VNFTESLLRMAADDVEEYMIERPEPEFQDLNEKA RALKQILSKIPDEINDRVRFLQTIKDIASAIKE LLDTVNNVFKKYQYQNRRVRLVEALLVDTLDVG LCLVVLLT*K*D*LLEIVLCH*PLSLFLNLNMPF YLKVSTPQLLISNIHIKCIPYPV*SLVLAFGFC DYFSYLVLYFLLY

Frame 2

TPAGQDSGQRLVPLGSFRRRRSELIRELKRAAR WEVKSVPQLLKDLTLLGVIPKISVFFVSNSFIT KKEKKYCSE*RFLCILALLFQL*LAFE*G*QWK R*RMKLRPHPWFLCPSMQSCILCLMS*NE*ICLQ PRH*EPLSSRLKKKIQVSHKTSL*KF*RKKAWK LTSRSPFFVWQLMM*KSI*LNDQSQNSKT*TKR HEHLNKFSVRSQMRSMTE*GFCRQSRI*LVQ*KN FLIQ*IMSSRNINTRTAGYV**KPC*LTP*MLV YA*LFY*LKNKIDCWK*FCVINH*VYS*I*ICL FI*K*VLHNCLLVIFI*SASHILCNLLSLPLAFV IISLI*YFISYYI

Sequence PDCD10.dAug05

GTAAGGTGTAGCTGTTCTGTCCCAGTCCCCGTAGCTCTGCACCGAGCAGAAGAGGTCTAGGGTCACGCAGGGGCCATGGCTGAGGCACTTGGGCTTGGTT CTGGCAGGCGGAGCGGACGGGGCTGAGAGCGGGGCCCTACGGGCGGAAGAGGAGCACTGGAAGAAGGAAGAGACAAATGTTGGGGTCTACGGATTCAGTT CCAGTTTAATGATGTGGCCCTCTAAGGAGATAATTTTGCCTTAAACAGAAAGACTTGACCCTCTTAGGTGTCATACCTAAGATCAGTGTGTTTTTTGTGT CCAATTCTTTTATCACCAAAAAAGAGAAGAAATATTGCAGTGAATGAAGATTCCTCTGCATTTTAGCACTGCTTTTTCAACTGTAGTTGGCTTTTGAATG AGGATGACAATGGAAGAGATGAAGAATGAAGCTGAGACCACATCCATGGTTTCTATGCCCCTCTATGCAGTCATGTATCCTGTGTTTAATGAGCTAGAAC GAGTAAATCTGTCTGCAGCCCAGACACTGAGAGCCGCTTTCATCAAGGCTGAAAAAGAAAATCCAGGTCTCACACAAGACATCATTATGAAAATTTTAGA GAAAAAAAGCGTGGAAGTTAACTTCACGGAGTCCCTTCTTCGTATGGCAGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCCAA GACCTAAACGAAAAGGCACGAGCACTTAAACAAATTCTCAGTAAGATCCCAGATGAGATCAATGACAGAGTGAGGTTTCTGCAGACAATCAAGGATATAG CTAGTGCAATAAAAGAACTTCTTGATACAGTGAATAATGTCTTCAAGAAATATCAATACCAGAACCGCAGGGCACTTGAACACCAAAAGAAAGAATTTGT AAAGTACTCCAAAAGTTTCAGTGATACTCTGAAAACGTATTTTAAAGATGGCAAGGCAATAAATGTGTTCGTAAGTGCCAACCGACTAATTCATCAAACC AACTTAATACTTCAGACCTTCAAAACTGTGGCCTGAAAGTTGTATATGTTAAGAGATGTACTTCTCAGTGGCAGTATTGAACTGCCTTTATCTGTAAATT TTAAAGTTTGACTGTATAAATTATCAGTCCCTCCTGAAGGGATCTAATCCAGGATGTTGAATGGGATTATTGCCATCTTACACCATATTTTTGTAAAATG TAGCTTAATCATAATCTCACACTGAAGATTTTGCATCACTTTTGCTATTATCATTCTTTTAAGAATTATAAGCCAAAAGAATTTACGCCTTAATGTGTCA TTATATAACATTCCTTAAAAGAATTGTAAATATTGGTGTTTGTTTCTGACATTTTAACTTGAAAGCGATATGCTGCAAGATAATGTATTTAACAATATTT GGTGGCAAATATTCAATAAATAGTTTACATCTGTTAAACATTTCTTTACTTGAAAAAATGCATATATATATGTGTGTATATATATATATATATATATATA TATGAACAGAAGACCAAGACAACTTGGTGTAATGTCTAAAAAACTTCTAATGGGAGCTTATCAGCAGTTTATCAAATAATAAAGCAAGAATTTCTATTTT TGTCCTTAATCACTTTGGACACAGAGTAGTCAATGGAAATATTTTTGTTAATTACTTATAAGGTACTGAGTATTTTTATATGATTTCCAACCTGTTTTAA ACGACTTATGAGTAAGAGATAGCATTTTCTAGTGTTTTTGCACTATAATGCCACTTTTTGAACTCTTGTGACAAATGTAGATTTGACTCTTAAAAATTAA GCCTTTCGTATACTGGGCCCTCATAAGCTTTGCACAATTTTTTCCCAAAAAATAGCCACAATTAATAGTAATTTGAAGCTATATCAACGGTTGGATTGAA ATTGTCATGAGTGTTAATATTATATACACTCTTCAACTAGGTACAGTTTTATTTCACCATAGAGAAGTTTTAAGGAAATTATGTATCAAGTGTTCATGTT AAGTGGTTTCATTTTTCAATTATAAAATAAAGGG

PDCD10.u.f.21 (forward primer)
PDCD10.u.r.7 (reverse primer)
Single exon skipping

Frame 0

VRCSCSVPVPVALHRAEEV*GHAGAMAEALGLG SGRRSGRG*ERGPTGGRGALEEGRDKCWGLRIQF QFNDVAL*GDNFALNRKT*PS*VSYLRSVCFLC PILLSPKKRRNIAVNEDSSAF*HCFFNCSWLLN EDDNGRDEE*S*DHIHGFYAPLCSHVSCV**ART SKSVCSPDTESRFHQG*KRKSRSHTRHHYENFR EKKRGS*LHGVPSSYGS**CRRVYD*TTRARIP RPKRKGTST*TNSQ*DPR*DQ*QSEVSADNQGYS *CNKRTS*YSE*CLQEISIPEPQGT*TPKERIC KVLQKFQ*YSENVF*RWQGNKCVRKCQPTNSSN QLNTSDLQNCGLKVVYVKRCTSQWQY*TAFICKF *SLTV*IISPS*RDLIQDVEWDYCHLTPYFCKM *LNHNLTLKILHHFCYYHSFKNYKPKEFTP*CV II*HSLKEL*ILVFVSDILT*KRYAAR*CI*QYL VANIQ*IVYIC*TFLYLKKCIYICVYIYIYIYI YEQKTKTTWCNV*KTSNGSLSAVYQIIKQEFLF LSLITLDTE*SMEIFLLITYKVLSIFI*FPTCFK RLMSKR*HFLVFLHYNATF*TLVTNVDLTLKN* AFRILGPHKLCTIFSQKIATINSNLKLYQRLD* NCHEC*YYIHSSTRYSFISP*RSFKEIMYQVFML SGFIFQL*NKG

Frame 1

*GVAVLSQSP*LCTEQKRSRVTQGPWLRHLGLV LAGGADGAESGALRAEEEHWKKEETNVGVYGFS SSLMMWPSKEIILP*TERLDPLRCHT*DQCVFCV QFFYHQKREEILQ*MKIPLHFSTAFSTVVGF*M RMTMEEMKNEAETTSMVSMPLYAVMYPVFNELE RVNLSAAQTLRAAFIKAEKENPGLTQDIIMKILE KKSVEVNFTESLLRMAADDVEEYMIERPEPEFQ DLNEKARALKQILSKIPDEINDRVRFLQTIKDI ASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFV KYSKSFSDTLKTYFKDGKAINVFVSANRLIHQT NLILQTFKTVA*KLYMLRDVLLSGSIELPLSVN FKV*LYKLSVPPEGI*SRMLNGIIAILHHIFVKC SLIIISH*RFCITFAIIILLRIISQKNLRLNVS LYNIP*KNCKYWCLFLTF*LESDMLQDNVFNNI WWQIFNK*FTSVKHFFT*KNAYIYVCIYIYIYIY MNRRPRQLGVMSKKLLMGAYQQFIK**SKNFYF CP*SLWTQSSQWKYFC*LLIRY*VFLYDFQPVL NDL*VRDSIF*CFCTIMPLFELL*QM*I*LLKIK PFVYWALISFAQFFPKK*PQLIVI*SYINGWIE IVMSVNIIYTLQLGTVLFHHREVLRKLCIKCSC *VVSFFNYKIK

Frame 2

KV*LFCPSPRSSAPSRRGLGSRRGHG*GTWAWF WQAERTGLRAGPYGRKRSTGRRKRQMLGSTDSV PV**CGPLRR*FCLKQKDLTLLGVIPKISVFFV SNSFITKKEKKYCSE*RFLCILALLFQL*LAFE* G*QWKR*RMKLRPHPWFLCPSMQSCILCLMS*N E*ICLQPRH*EPLSSRLKKKIQVSHKTSL*KF* RKKAWKLTSRSPFFVWQLMM*KSI*LNDQSQNSK T*TKRHEHLNKFSVRSQMRSMTE*GFCRQSRI* LVQ*KNFLIQ*IMSSRNINTRTAGHLNTKRKNL *STPKVSVIL*KRILKMARQ*MCS*VPTD*FIKP T*YFRPSKLWPESCIC*EMYFSVAVLNCLYL*I LKFDCINYQSLLKGSNPGC*MGLLPSYTIFL*N VA*S*SHTEDFASLLLLSFF*EL*AKRIYALMCH YITFLKRIVNIGVCF*HFNLKAICCKIMYLTIF GGKYSINSLHLLNISLLEKMHIYMCVYIYIYIY I*TEDQDNLV*CLKNF*WELISSLSNNKARISIF VLNHFGHRVVNGNIFVNYL*GTEYFYMISNLF* TTYE*EIAFSSVFAL*CHFLNSCDKCRFDS*KL SLSYTGPS*ALHNFFPKNSHN***FEAISTVGLK LS*VLILYTLFN*VQFYFTIEKF*GNYVSSVHV KWFHFSIIK*R

Sequence PDCD10.cAug05

CGACTCCGGCTGGGCAGGATTCCGGACAACGCCTGGTTCCTCTTGGGTCCTTCCGGCGTCGCCGGAGTGAATTGATCCGGGAGTTGAAGAGGGCTGCAAG GTGGGAAGTGAAGTCAGTGCCTCAGTTGCTGGGAAGAGGAGCACTGGAAGAAGGAAGAGACAAATGTTGGGGTCTACGGATTCAGTTCCAGTTTAATGAT GTGGCCCTCTAAGGAGATAATTTTGCCTTAAACAGAAAGACTTGACCCTCTTAGGTGTCATACCTAAGATCAGTGTGTTTTTTGTGTCCAATTCTTTTAT CACCAAAAAAGAGAAGAAATATTGCAGTGAATGAAGATTCCTCTGCATTTTAGCACTGCTTTTTCAACTGTAGTTGGCTTTTGAATGAGGATGACAATGG AAGAGATGAAGAATGAAGCTGAGACCACATCCATGGTTTCTATGCCCCTCTATGCAGTCATGTATCCTGTGTTTAATGAGCTAGAACGAGTAAATCTGTC TGCAGCCCAGACACTGAGAGCCGCTTTCATCAAGGCTGAAAAAGAAAATCCAGGTCTCACACAAGACATCATTATGAAAATTTTAGAGAAAAAAAGCGTG GAAGTTAACTTCACGGAGTCCCTTCTTCGTATGGCAGCTGATGATGTAGAAGAGTATATGATTGAACGACCAGAGCCAGAATTCCAAGACCTAAACGAAA AGGCACGAGCACTTAAACAAATTCTCAGTAAGATCCCAGATGAGATCAATGACAGAGTGAGGTTTCTGCAGACAATCAAGGATATAGCTAGTGCAATAAA AGAACTTCTTGATACAGTGAATAATGTCTTCAAGAAATATCAATACCAGAACCGCAGGGCACTTGAACACCAAAAGAAAGAATTTGTAAAGTACTCCAAA AGTTTCAGTGATACTCTGAAAACGTATTTTAAAGATGGCAAGGCAATAAATGTGTTCGTAAGTGCCAACCGACTAATTCATCAAACCAACTTAATACTTC AGACCTTCAAAACTGTGGCCTGAAAGTTGTATATGTTAAGAGATGTACTTCTCAGTGGCAGTATTGAACTGCCTTTATCTGTAAATTTTAAAGTTTGACT GTATAAATTATCAGTCCCTCCTGAAGGGATCTAATCCAGGATGTTGAATGGGATTATTGCCATCTTACACCATATTTTTGTAAAATGTAGCTTAATCATA ATCTCACACTGAAGATTTTGCATCACTTTTGCTATTATCATTCTTTTAAGAATTATAAGCCAAAAGAATTTACGCCTTAATGTGTCATTATATAACATTC CTTAAAAGAATTGTAAATATTGGTGTTTGTTTCTGACATTTTAACTTGAAAGCGATATGCTGCAAGATAATGTATTTAACAATATTTGGTGGCAAATATT CAATAAATAGTTTACATCTGTTAAACATTTCTTTACTTGAAAAAATGCATATATATATGTGTGTATATATATATATATATATATATATATGAACAGAAGA CCAAGACAACTTGGTGTAATGTCTAAAAAACTTCTAATGGGAGCTTATCAGCAGTTTATCAAATAATAAAGCAAGAATTTCTATTTTTGTCCTTAATCAC TTTGGACACAGAGTAGTCAATGGAAATATTTTTGTTAATTACTTATAAGGTACTGAGTATTTTTATATGATTTCCAACCTGTTTTAAACGACTTATGAGT AAGAGATAGCATTTTCTAGTGTTTTTGCACTATAATGCCACTTTTTGAACTCTTGTGACAAATGTAGATTTGACTCTTAAAAATTAAGCCTTTCGTATAC TGGGCCCTCATAAGCTTTGCACAATTTTTTCCCAAAAAATAGCCACAATTAATAGTAATTTGAAGCTATATCAACGGTTGGATTGAAATTGTCATGAGTG TTAATATTATATACACTCTTCAACTAGGTACAGTTTTATTTCACCATAGAGAAGTTTTAAGGAAATTATGTATCAAGTGTTCATGTTAAGTGGTTTCATT TTTCAATTATAAAATAAAGGG

PDCD10.u.f.21 (forward primer)
PDCD10.u.r.7 (reverse primer)
Single exon skipping

Frame 0

RLRLGRIPDNAWFLLGPSGVAGVN*SGS*RGLQ GGK*SQCLSCWEEEHWKKEETNVGVYGFSSSLMM WPSKEIILP*TERLDPLRCHT*DQCVFCVQFFY HQKREEILQ*MKIPLHFSTAFSTVVGF*MRMTM EEMKNEAETTSMVSMPLYAVMYPVFNELERVNLS AAQTLRAAFIKAEKENPGLTQDIIMKILEKKSV EVNFTESLLRMAADDVEEYMIERPEPEFQDLNE KARALKQILSKIPDEINDRVRFLQTIKDIASAIK ELLDTVNNVFKKYQYQNRRALEHQKKEFVKYSK SFSDTLKTYFKDGKAINVFVSANRLIHQTNLIL QTFKTVA*KLYMLRDVLLSGSIELPLSVNFKV*L YKLSVPPEGI*SRMLNGIIAILHHIFVKCSLII ISH*RFCITFAIIILLRIISQKNLRLNVSLYNI P*KNCKYWCLFLTF*LESDMLQDNVFNNIWWQIF NK*FTSVKHFFT*KNAYIYVCIYIYIYIYMNRR PRQLGVMSKKLLMGAYQQFIK**SKNFYFCP*S LWTQSSQWKYFC*LLIRY*VFLYDFQPVLNDL*V RDSIF*CFCTIMPLFELL*QM*I*LLKIKPFVY WALISFAQFFPKK*PQLIVI*SYINGWIEIVMS VNIIYTLQLGTVLFHHREVLRKLCIKCSC*VVSF FNYKIK

Frame 1

DSGWAGFRTTPGSSWVLPASPE*IDPGVEEGCK VGSEVSASVAGKRSTGRRKRQMLGSTDSVPV** CGPLRR*FCLKQKDLTLLGVIPKISVFFVSNSFI TKKEKKYCSE*RFLCILALLFQL*LAFE*G*QW KR*RMKLRPHPWFLCPSMQSCILCLMS*NE*IC LQPRH*EPLSSRLKKKIQVSHKTSL*KF*RKKAW KLTSRSPFFVWQLMM*KSI*LNDQSQNSKT*TK RHEHLNKFSVRSQMRSMTE*GFCRQSRI*LVQ* KNFLIQ*IMSSRNINTRTAGHLNTKRKNL*STPK VSVIL*KRILKMARQ*MCS*VPTD*FIKPT*YF RPSKLWPESCIC*EMYFSVAVLNCLYL*ILKFD CINYQSLLKGSNPGC*MGLLPSYTIFL*NVA*S* SHTEDFASLLLLSFF*EL*AKRIYALMCHYITF LKRIVNIGVCF*HFNLKAICCKIMYLTIFGGKY SINSLHLLNISLLEKMHIYMCVYIYIYIYI*TED QDNLV*CLKNF*WELISSLSNNKARISIFVLNH FGHRVVNGNIFVNYL*GTEYFYMISNLF*TTYE *EIAFSSVFAL*CHFLNSCDKCRFDS*KLSLSYT GPS*ALHNFFPKNSHN***FEAISTVGLKLS*V LILYTLFN*VQFYFTIEKF*GNYVSSVHVKWFH FSIIK*R

Frame 2

TPAGQDSGQRLVPLGSFRRRRSELIRELKRAAR WEVKSVPQLLGRGALEEGRDKCWGLRIQFQFND VAL*GDNFALNRKT*PS*VSYLRSVCFLCPILL SPKKRRNIAVNEDSSAF*HCFFNCSWLLNEDDNG RDEE*S*DHIHGFYAPLCSHVSCV**ARTSKSV CSPDTESRFHQG*KRKSRSHTRHHYENFREKKR GS*LHGVPSSYGS**CRRVYD*TTRARIPRPKRK GTST*TNSQ*DPR*DQ*QSEVSADNQGYS*CNK RTS*YSE*CLQEISIPEPQGT*TPKERICKVLQ KFQ*YSENVF*RWQGNKCVRKCQPTNSSNQLNTS DLQNCGLKVVYVKRCTSQWQY*TAFICKF*SLT V*IISPS*RDLIQDVEWDYCHLTPYFCKM*LNH NLTLKILHHFCYYHSFKNYKPKEFTP*CVII*HS LKEL*ILVFVSDILT*KRYAAR*CI*QYLVANI Q*IVYIC*TFLYLKKCIYICVYIYIYIYIYEQK TKTTWCNV*KTSNGSLSAVYQIIKQEFLFLSLIT LDTE*SMEIFLLITYKVLSIFI*FPTCFKRLMS KR*HFLVFLHYNATF*TLVTNVDLTLKN*AFRI LGPHKLCTIFSQKIATINSNLKLYQRLD*NCHEC *YYIHSSTRYSFISP*RSFKEIMYQVFMLSGFI FQL*NKG

Sequence PDCD10.oAug05

CGACTCCGGCTGGGCAGGATTCCGGACAACGCCTGGTTCCTCTTGGGTCCTTCCGGCGTCGCCGGAGTGAATTGATCCGGGAGTTGAAGAGGGCTGCAAG GTGGGAAGTGAAGTCAGTGCCTCAGTTGCTGAGGAGCACTGGAAGAAGGAAGAGACAAATGTTGGGGTCTACGGATTCAGTTCCAGTTTAATGATGTGGC CCTCTAAGGAGATAATTTTGCCTTAAACAGAAAGACTTGACCCTCTTAGGTGTCATACCTAAGCTTTCTCCTCTAAATCTTGGTCTCTTGTTTGGAAGTG ACTAACAGCATTTTGGCATGAGAAAGCCCTAAAAAGATCAGTGTGTTTTTTGTGTCCAATTCTTTTATCACCAAAAAAGAGAAGAAATATTGCAGTGAAT GAAGATTCCTCTGCATTTTAGCACTGCTTTTTCAACTGTAGTTGGCTTTTGAATGAGGATGACAATGGAAGAGATGAAGAATGAAGCTGAGACCACATCC ATGGTTTCTATGCCCCTCTATGCAGTCATGTATCCTGTGTTTAATGAGCTAGAACGAGTAAATCTGTCTGCAGCCCAGACACTGAGAGCCGCTTTCATCA AGGCTGAAAAAGAAAATCCAGGTCTCACACAAGACATCATTATGAAAATTTTAGAGAAAAAAAGCGTGGAAGTTAACTTCACGGAGTCCCTTCTTCGTAT GGCAGCTGATGATGTAGAAG

PDCD10.u.f.21 (forward primer)
PDCD10.u.r.7 (reverse primer)
Single exon skipping

Frame 0

RLRLGRIPDNAWFLLGPSGVAGVN*SGS*RGLQ GGK*SQCLSC*GALEEGRDKCWGLRIQFQFNDVA L*GDNFALNRKT*PS*VSYLSFLL*ILVSCLEV TNSILA*ESPKKISVFFVSNSFITKKEKKYCSE *RFLCILALLFQL*LAFE*G*QWKR*RMKLRPHP WFLCPSMQSCILCLMS*NE*ICLQPRH*EPLSS RLKKKIQVSHKTSL*KF*RKKAWKLTSRSPFFV WQLMM*K

Frame 1

DSGWAGFRTTPGSSWVLPASPE*IDPGVEEGCK VGSEVSASVAEEHWKKEETNVGVYGFSSSLMMW PSKEIILP*TERLDPLRCHT*AFSSKSWSLVWK* LTAFWHEKALKRSVCFLCPILLSPKKRRNIAVN EDSSAF*HCFFNCSWLLNEDDNGRDEE*S*DHI HGFYAPLCSHVSCV**ARTSKSVCSPDTESRFHQ G*KRKSRSHTRHHYENFREKKRGS*LHGVPSSY GS**CR

Frame 2

TPAGQDSGQRLVPLGSFRRRRSELIRELKRAAR WEVKSVPQLLRSTGRRKRQMLGSTDSVPV**CG PLRR*FCLKQKDLTLLGVIPKLSPLNLGLLFGS D*QHFGMRKP*KDQCVFCVQFFYHQKREEILQ*M KIPLHFSTAFSTVVGF*MRMTMEEMKNEAETTS MVSMPLYAVMYPVFNELERVNLSAAQTLRAAFI KAEKENPGLTQDIIMKILEKKSVEVNFTESLLRM AADDVE