Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence NALP1.cAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAGTGAGCGTTGCCCACACCCCATCTCCCGTCACCACATCTCCCCTCACCCTCACCCTCCCTGCCTGGCCCTGGACCCCATCC CAGGACCTCCCTATCAGCTGACTTCTTCCAGTGTCTTGCAGGCCCCTCTGGGCTCCTCCCTCCCCTGGCTTTTCCTACCACTCCCCCTCTATCGGCGTCT ATCTGTAGGTGCCCTGGGATTTATAAAACTGGGTTCCGAATGCTGAATAAGAGACGGTAAGAGCCAAGGCAAAGGACAGCACTGTTCTCTGCCTGCCTGA TACCCTCACCACCTGGGAACATCCCCCAGACACCCTCTTAACTCCGGGACAGAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCC TGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAG TGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTG TGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAG TGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAG AGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTG CTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACT ACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACA GCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTA CTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAG ACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGA AGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAG CTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAG GATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACT TCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCT GAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCC TGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAAC CCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAA AAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCC CTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTG CATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGG GAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTC TGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACAT GGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACC ATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAA GTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCT CACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCC AAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCT CTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCC TGCCTGCAAACTCATACGCCTGGGGCTGGACCAGACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTC ATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCG GATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCC AGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGGGGCCCC ACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCT GCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGG GCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTC CAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGG GAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCA CCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGC CACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTGCAACTA CTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCTGCACTTTGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGT GACATCGGTGGAGGTTGTCTTGGACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCCCAGCCAGATG CGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAAGGAGACCCATCCTCACCTCATTATGGAACTCT GGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGCTGAAGTATCAACACCAGCCCTTGACCCTTGAGTCCTGGCTTTGGCTGACCCTTCTTTGG GTCTCAGTTTCTTTCTCTGCAAACAAGTTGCCATCTGGTTTGCCTTCCAGCACTAAAGTAATGGAACTTTGATGATGCCTTTGCTGGGCATTATGTGTCC ATGCCAGGGATGCCACAGGGGGCCCCAGTCCAGGTGGCCTAACAGCATCTCAGGGAATGTCCATCTGGAGCTGGCAAGACCCCTGCAGACCTCATAGAGC CTCATCTGGTGGCCACAGCAGCCAAGCCTAGAGCCCTCCGGATCCCATCCAGGCGCAAAGAGGAATAGGAGGGACATGGAACCATTTGCCTCTGGCTGTG TCACAGGGTGAGCCCCAAAATTGGGGTTCAGCGTGGGAGGCCACGTGGATTCTTGGCTTTGTACAGGAAGATCTACAAGAGCAAGCCAACAGAGTAAAGT GGAAGGAAGTTTATTCAGAAAATAAAGGAGTATCACAGCTCTTTTAGAATTTGTCTAGCAGGCTTTCCAGTTTTTACCAGAAAACCCCTATAAATTAAAA ATTTTTTACTTAAATTTAAGAATTAAAAAAATACAAAAAAGAAAAAATGAAAATAAAGGAATAAGAAGTTACCTACTCCAT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTVSVAHTPSPVTTSPLTLTLPAWPWTPS QDLPIS*LLPVSCRPLWAPPSPGFSYHSPSIGV YL*VPWDL*NWVPNAE*ETVRAKAKDSTVLCLPD TLTTWEHPPDTLLTPGQRWLAEPGAAWPVTWSS *RRRS*RSSSFCSPIKRTPGALRVRHPLSQRRR VAWRWPRTWWLSMGSSGPGT*PSIPGSRWG*GHC APKPRKGQATLPHSPTAQVNPTWGLPANPPPPQ C*CPGSMNCRRGAPRAQREGF*DSCLTHLDAAG EKSLPHSSTKLFQAPQTMSLQARSHPTPPHPQQC WGAGDPHLSPA*HPESRRLLGPNGLWMKRQEFT TQKSEKERERNQRKAGPHGQRW*ERPHRRTPAY SPTTTHGSLL*ERASVPHGPGKMRILTKNSHSCY FYKDLTPEAKIPWSREAGLIMWRRIEDI*LRSE TYLAQAWIPKNLA*SYCRGLLELGSQHWPGR*R KPGGEASCMGTASSMSSTSAAESWPSPRW*VSLS SSEKMGQPLRLPLDRSCLGQSGCSSSSMV*MSQ DGSCRSRVLSSVCTGASHSRRMHCWAVCWGKLY FPRHPS*SRLGPQLCRTSFLLWSRHVG*RSWGSL SPAGRNISTDISQMKGKQLEPLGWSNQTKSSGP CVLCPGCPGWPALA*CSR*SGRKNSH*LPRPPQ PSVYITLPRLSKLSHWDPSSETSALWLLRASGKK RPFSVQMTSGSMG*MGPSSPPS*RWVFFKSTPS L*ATASFTSVSKSSLQQCPMSWRMRRGEVNILI AS*IWKRR*KHMEYMACLGHQPHVSYWAC*VMRG RERWRTSFTAGCLRGGT*CSGSRPCSCCCSHTL WSPSTACTRLGTKRS*HK*WPISKKWACV*KQT WSS*CALSALNSAAT*RSFS*LRAGSTDQHGAPP W*SCSGGSQSQMPIGRFSSPSSRSPET*RSWT* VETR*ATLQ*RVFVRP*DALAASWRPCGWLAVA SQLRTARTLPLG*EPTRP*PSWT*ASMCSRMLEP NTFARD*DSRAASYSDCSWSAVASRLTAARTWP LCLVPAPA*RS*TCSRTTWMTLACDCSVRGSGI LPANSYAWGWTRQL*VMR*GRN*GPWSRRNLSCS SSADGNQV**PLLRAWIRER*VIAHPHSSGRDS DQRGRLPMLLRLISNSWT*ARSSQLLRLQRKAP QR*YRWNSCACLLLPLKGTCIRSLWGLTMTSGAP RGLWLLR*LTKKRTCTEFTSL*LAPTAGPTRVS AL**EKR*PLRLNSVCGTSSWVRSTHSTAGWWQ GLCWTSRLSLELWKLCTSLTLWLSKGAMWTHPCS KWPTLKRRGCSWRSQPGWSCIT*FWKTPASPPW ESS*K*SIMPCASFPSPLWCCFTTASILRKSPS TST*SQVTAPFGRNWSSAIEALEKTSCSRSSTLA TWDQGSGCK*KTRKMRLWCGRPW*NQEISCLQL L*SLQPA*PYLHLWMPRSCCTLWTSIESS**PE *HRWRLSWTNCMDRC*ARSSTRGCWLRTRGPARC GSCSA*ASPGTGSAKMDSTKP*RRPILTSLWNS GRRAAKRDSCHSAAEVSTPALDP*VLALADPSL GLSFFLCKQVAIWFAFQH*SNGTLMMPLLGIMCP CQGCHRGPQSRWPNSISGNVHLELARPLQTS*S LIWWPQQPSLEPSGSHPGAKRNRRDMEPFASGC VTG*APKLGFSVGGHVDSWLCTGRSTRASQQSKV EGSLFRK*RSITALLEFV*QAFQFLPENPYKLK IFYLNLRIKKIQKRKNENKGIRSYLLH

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQ*ALPTPHLPSPHLPSPSPSLPGPGPHP RTSLSADFFQCLAGPSGLLPPLAFPTTPPLSAS ICRCPGIYKTGFRMLNKRR*EPRQRTALFSACL IPSPPGNIPQTPS*LRDRDGWRSLGPPGLLLGVP EEGGAEGVPASARQ*SALQELFG*DTRSAREDE WHGGGLVPGGSVWGAAGLGPSPPYLGADGAEVT VRPSPGRGRPLSLIPLQPK*TPPGVSQPTHLHRS ANALDP*IAGGVHPGLREKGFETAA*HIWTPLE RNLCLTPLPSSSKLPRP*VSKPGVTQRPHIHSS AGELGIPTSAQPSTQRAGGSWDPMASG*NVRNLL HRNQRKREREIRERQAPMGSGGRNAPTGAHQPT APPPPMGAFCEREPLFHMALEK*GF*PKIHTAA TSTKTSPQKPRSPGQEKLA*LCGGESRTFN*DQR LIWPRPGYPRTSHSHTAGGCWNWEVNTGQAGEG SLGERPAVWGPLPACLLLQLQRAGPVQGGESR* AHRKRWDSHSGSH*TDPV*ARAAALHPRWCR*AR MGLAGAEF*ALSALEPATAGGCTAGQFAGENYT SRGILPDHGSDHSSAEPHSFFGAGTLGRGPGVL *VQQEGIFLQIFHR*KASN*SL*VGQIKQRALGP VSCALGVLAGLHLPDAADEAEGKTHTDFQDHHN PLSTLPCPGSPSSAIGTPAQRPLLSGC*GHLAK KDPFQSR*PQEAWVRWGHHLHLLEDGYSSRAPHP SELQLHSPLFPRVLCSNVLCLGG*EGER*TF*L HHRFGKDARSIWNTWPVWGINHTFPIGPVK**G GERDGEHLSLPAVSGEEPDAVGPVPAAAAAATLS GVPPLLVRDSEQNVPDTSDGPFRRNGHVCRNRH GALSVHFLH*IQPPREEASAD*GQAAQINMEPH HGSPVQVGPSHRCLLADSLLRPQGHQKPEGAGPK WKLAEPLCSEESL*DPETPSLPPGDPAVGWLWP HS*GLQGPCLWAESQPDPDRAGPELQCAHGCWS QTPLPETETAELQATATAAGQLWPHV*LLPGPGL CA*CQPQPEGARPAAEQPG*RWRATAL*GAQAS CLQTHTPGAGPDNSE**DEAGTEGPGAGETSAA HLQQTETKCDDPY*GPGYGRDE**HILTQAAETR IREGGFPCCSG*SQTPGREQDLPNC*DCRGKLP RGSTGGTLVRAFSCLSRGPAYEAFGD*R*LLGP HGACGY*GS*QRKELVPSSLPCSWLLPLAQHGSL LCDERSGDR*D*ILCVGPVPG*DQPTAQLDGGR ASAGHQG*AWSCGSCAPPSLCGSPRGPCGHIPV PNGPL*RGGDAPGEASQGGAASHSSGKPQLLPLG SPPENDP*CPALHSRHLCGVALPPRPS*GSHLP PLPDPK*LLHSEGTGALLSKPWRRPAVLGVLRW PLGIRDQAASERQER*DSGVGGLGETRRSHACNY SDPSSPHSRTFTSGCPAVAALCGPVSRAADSPS DIGGGCLGQTAWTGAEPGAVREGAG*EHEAQPD AEAVQLEPVLGPEVQRWTLPSPEGDPSSPHYGTL GEGQQKGTPATQQLKYQHQPLTLESWLWLTLLW VSVSFSANKLPSGLPSSTKVMEL**CLCWALCV HARDATGGPSPGGLTASQGMSIWSWQDPCRPHRA SSGGHSSQA*SPPDPIQAQRGIGGTWNHLPLAV SQGEPQNWGSAWEATWILGFVQEDLQEQANRVK WKEVYSENKGVSQLF*NLSSRLSSFYQKTPIN*K FFT*I*ELKKYKKEKMKIKE*EVTYS

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNSERCPHPISRHHISPHPHPPCLALDPI PGPPYQLTSSSVLQAPLGSSLPWLFLPLPLYRRL SVGALGFIKLGSEC*IRDGKSQGKGQHCSLPA* YPHHLGTSPRHPLNSGTEMAGGAWGRLACYLEF LKKEELKEFQLLLANKAHSRSSSGETPAQPEKTS GMEVASYLVAQYGEQRAWDLALHTWEQMGLRSL CAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTA VLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWR EISASLLYQALPSSPDHESPSQESPNAPTSTAV LGSWGSPPQPSLAPREQEAPGTQWPLDETSGIY YTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQ PHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLL LLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIR DLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKE AWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAE LIGKDGTATPAPIRQILSRPERLLFILDGVDEP GWVLQEPSSELCLHWSQPQPADALLGSLLGKTIL PEASFLITARTTALQNLIPSLEQARWVEVLGFS ESSRKEYFYRYFTDERQAIRAFRLVKSNKELWA LCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTT LCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQK KTLFSPDDLRKHGLDGAIISTFLKMGILQEHPI PLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNC IIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHS LESLHCLYETRNKTFLTQVMAHFEEMGMCVETDM ELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPT MVVLFRWVPVTDAYWQILFSVLKVTRNLKELDL SGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGL TAEDCKDLAFGLRANQTLTELDLSFNVLTDAGA KHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLA SVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHP ACKLIRLGLDQTTLSDEMRQELRALEQEKPQLL IFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRL GSERAASHVAQANLKLLDVSKIFPIAEIAEESSP EVVPVELLCVPSPASQGDLHTKPLGTDDDFWGP TGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGL CFVMREAVTVEIEFCVWDQFLGEINPQHSWMVAG PLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLF QMAHFKEEGMLLEKPARVELHHIVLENPSFSPL GVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFH LYLIPSDCSIRKELELCYRSPGEDQLFSEFYVG HLGSGIRLQVKDKKDETLVWEALVKPGDLMPAT TLIPPARIAVPSPLDAPQLLHFVDQYREQLIARV TSVEVVLDKLHGQVLSQEQYERVLAENTRPSQM RKLFSLSQSWDRKCKDGLYQALKETHPHLIMEL WEKGSKKGLLPLSS*SINTSP*PLSPGFG*PFFG SQFLSLQTSCHLVCLPALK*WNFDDAFAGHYVS MPGMPQGAPVQVA*QHLRECPSGAGKTPADLIE PHLVATAAKPRALRIPSRRKEE*EGHGTICLWLC HRVSPKIGVQRGRPRGFLALYRKIYKSKPTE*S GRKFIQKIKEYHSSFRICLAGFPVFTRKPL*IK NFLLKFKN*KNTKKKK*K*RNKKLPTP

Sequence NALP1.dAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCCTGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTC TGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAGTGGCATGGAGGTGGCCTCGTACCTGGTGGCTCA GTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTGTGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCT CCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAGTGCTAATGCCCTGGATCCATGAATTGCCGGCGG GGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAGAGAAATCTCTGCCTCACTCCTCTACCAAGCTCT TCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTGCTGGGGAGCTGGGGATCCCCACCTCAGCCCAGC CTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACTACACAGAAATCAGAGAAAGAGAGAGAGAGAAAT CAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACAGCCCCACCACCACCCATGGGAGCCTTCTGTGAG AGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTACTTCTACAAAGACCTCACCCCAGAAGCCAAGAT CCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAGACTTATTTGGCCCAGGCCTGGATACCCAAGAAC CTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGAAGCCTGGGGGAGAGGCCAGCTGTATGGGGACCG CTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAGCTCATCGGAAAAGATGGGACAGCCACTCCGGCT CCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAGGATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCT GTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACTTCCCGAGGCATCCTTCCTGATCACGGCTCGGAC CACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCTGAGTCCAGCAGGAAGGAATATTTCTACAGATAT TTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCCTGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCT GCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAACCCTCTGTCTACATTACCTTGCCCAGGCTCTCCA AGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAAAAGACCCTTTTCAGTCCAGATGACCTCAGGAAG CATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCCCTCTGAGCTACAGCTTCATTCACCTCTGTTTCC AAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTGCATCATAGATTTGGAAAAGACGCTAGAAGCATA TGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGGGAGAGAGAGATGGAGAACATCTTTCACTGCCGG CTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTCTGGAGTCCCTCCACTGCTTGTACGAGACTCGGA ACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACATGGAGCTCTTAGTGTGCACTTTCTGCATTAAATT CAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACCATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACA GATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAAGTGGAAACTCGCTGAGCCACTCTGCAGTGAAGA GTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCTCACAGCTGAGGACTGCAAGGACCTTGCCTTTGG GCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCCAAACACCTTTGCCAGAGACTGAGACAGCCGAGC TGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCTCTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGC TAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCCTGCCTGCAAACTCATACGCCTGGGGCTGGACCA GACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTCATCTTCAGCAGACGGAAACCAAGTGTGATGACC CCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGG CTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTGTGCGTGCC TTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAA GAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGA TTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGC TGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTC CTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGC GCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCG GAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAA GACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTT CACCTCTGGATGCCCCGCAGTTGCTGCACTTTGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCA TGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCCCAGCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGAC CGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAAGGAGACCCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCAC TCAGCAGCTGAAGTATCAACACCAGCCCTTGACCCTTGAGTCCTGGCTTTGGCTGACCCTTCTTTGGGTCTCAGTTTCTTTCTCTGCAAACAAGTTGCCA TCTGGTTTGCCTTCCAGCACTAAAGTAATGGAACTTTGATGATGCCTTTGCTGGGCATTATGTGTCCATGCCAGGGATGCCACAGGGGGCCCCAGTCCAG GTGGCCTAACAGCATCTCAGGGAATGTCCATCTGGAGCTGGCAAGACCCCTGCAGACCTCATAGAGCCTCATCTGGTGGCCACAGCAGCCAAGCCTAGAG CCCTCCGGATCCCATCCAGGCGCAAAGAGGAATAGGAGGGACATGGAACCATTTGCCTCTGGCTGTGTCACAGGGTGAGCCCCAAAATTGGGGTTCAGCG TGGGAGGCCACGTGGATTCTTGGCTTTGTACAGGAAGATCTACAAGAGCAAGCCAACAGAGTAAAGTGGAAGGAAGTTTATTCAGAAAATAAAGGAGTAT CACAGCTCTTTTAGAATTTGTCTAGCAGGCTTTCCAGTTTTTACCAGAAAACCCCTATAAATTAAAAATTTTTTACTTAAATTTAAGAATTAAAAAAATA CAAAAAAGAAAAAATGAAAATAAAGGAATAAGAAGTTACCTACTCCAT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTRWLAEPGAAWPVTWSS*RRRS*RSSSF CSPIKRTPGALRVRHPLSQRRRVAWRWPRTWWL SMGSSGPGT*PSIPGSRWG*GHCAPKPRKGQATL PHSPTAQVNPTWGLPANPPPPQC*CPGSMNCRR GAPRAQREGF*DSCLTHLDAAGEKSLPHSSTKL FQAPQTMSLQARSHPTPPHPQQCWGAGDPHLSPA *HPESRRLLGPNGLWMKRQEFTTQKSEKERERN QRKAGPHGQRW*ERPHRRTPAYSPTTTHGSLL* ERASVPHGPGKMRILTKNSHSCYFYKDLTPEAKI PWSREAGLIMWRRIEDI*LRSETYLAQAWIPKN LA*SYCRGLLELGSQHWPGR*RKPGGEASCMGT ASSMSSTSAAESWPSPRW*VSLSSSEKMGQPLRL PLDRSCLGQSGCSSSSMV*MSQDGSCRSRVLSS VCTGASHSRRMHCWAVCWGKLYFPRHPS*SRLG PQLCRTSFLLWSRHVG*RSWGSLSPAGRNISTDI SQMKGKQLEPLGWSNQTKSSGPCVLCPGCPGWP ALA*CSR*SGRKNSH*LPRPPQPSVYITLPRLS KLSHWDPSSETSALWLLRASGKKRPFSVQMTSGS MG*MGPSSPPS*RWVFFKSTPSL*ATASFTSVS KSSLQQCPMSWRMRRGEVNILIAS*IWKRR*KH MEYMACLGHQPHVSYWAC*VMRGRERWRTSFTAG CLRGGT*CSGSRPCSCCCSHTLWSPSTACTRLG TKRS*HK*WPISKKWACV*KQTWSS*CALSALN SAAT*RSFS*LRAGSTDQHGAPPW*SCSGGSQSQ MPIGRFSSPSSRSPET*RSWT*VETR*ATLQ*R VFVRP*DALAASWRPCGWLAVASQLRTARTLPL G*EPTRP*PSWT*ASMCSRMLEPNTFARD*DSRA ASYSDCSWSAVASRLTAARTWPLCLVPAPA*RS *TCSRTTWMTLACDCSVRGSGILPANSYAWGWT RQL*VMR*GRN*GPWSRRNLSCSSSADGNQV**P LLRAWIRER*VIAHPHSSGRDSDQRGRLPMLLR LISNSWT*ARSSQLLRLQRKAPQR*YRWNSCAC LLLPLKGTCIRSLWGLTMTSGAPRGLWLLR*LTK KRTCTEFTSL*LAPTAGPTRVSAL**EKR*PLR LNSVCGTSSWVRSTHSTAGWWQGLCWTSRLSLE LWKLCTSLTLWLSKGAMWTHPCSKWPTLKRRGCS WRSQPGWSCIT*FWKTPASPPWESS*K*SIMPC ASFPSPLWCCFTTASILRKSPSTST*SQVTAPF GRNWSSAIEALEKTSCSRSSTLATWDQGSGCK*K TRKMRLWCGRPW*NQEISCLQLL*SLQPA*PYL HLWMPRSCCTLWTSIESS**PE*HRWRLSWTNC MDRC*ARSSTRGCWLRTRGPARCGSCSA*ASPGT GSAKMDSTKP*RRPILTSLWNSGRRAAKRDSCH SAAEVSTPALDP*VLALADPSLGLSFFLCKQVA IWFAFQH*SNGTLMMPLLGIMCPCQGCHRGPQSR WPNSISGNVHLELARPLQTS*SLIWWPQQPSLE PSGSHPGAKRNRRDMEPFASGCVTG*APKLGFS VGGHVDSWLCTGRSTRASQQSKVEGSLFRK*RSI TALLEFV*QAFQFLPENPYKLKIFYLNLRIKKI QKRKNENKGIRSYLLH

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQDGWRSLGPPGLLLGVPEEGGAEGVPAS ARQ*SALQELFG*DTRSAREDEWHGGGLVPGGS VWGAAGLGPSPPYLGADGAEVTVRPSPGRGRPL SLIPLQPK*TPPGVSQPTHLHRSANALDP*IAGG VHPGLREKGFETAA*HIWTPLERNLCLTPLPSS SKLPRP*VSKPGVTQRPHIHSSAGELGIPTSAQ PSTQRAGGSWDPMASG*NVRNLLHRNQRKREREI RERQAPMGSGGRNAPTGAHQPTAPPPPMGAFCE REPLFHMALEK*GF*PKIHTAATSTKTSPQKPR SPGQEKLA*LCGGESRTFN*DQRLIWPRPGYPRT SHSHTAGGCWNWEVNTGQAGEGSLGERPAVWGP LPACLLLQLQRAGPVQGGESR*AHRKRWDSHSG SH*TDPV*ARAAALHPRWCR*ARMGLAGAEF*AL SALEPATAGGCTAGQFAGENYTSRGILPDHGSD HSSAEPHSFFGAGTLGRGPGVL*VQQEGIFLQI FHR*KASN*SL*VGQIKQRALGPVSCALGVLAGL HLPDAADEAEGKTHTDFQDHHNPLSTLPCPGSP SSAIGTPAQRPLLSGC*GHLAKKDPFQSR*PQE AWVRWGHHLHLLEDGYSSRAPHPSELQLHSPLFP RVLCSNVLCLGG*EGER*TF*LHHRFGKDARSI WNTWPVWGINHTFPIGPVK**GGERDGEHLSLP AVSGEEPDAVGPVPAAAAAATLSGVPPLLVRDSE QNVPDTSDGPFRRNGHVCRNRHGALSVHFLH*I QPPREEASAD*GQAAQINMEPHHGSPVQVGPSH RCLLADSLLRPQGHQKPEGAGPKWKLAEPLCSEE SL*DPETPSLPPGDPAVGWLWPHS*GLQGPCLW AESQPDPDRAGPELQCAHGCWSQTPLPETETAE LQATATAAGQLWPHV*LLPGPGLCA*CQPQPEGA RPAAEQPG*RWRATAL*GAQASCLQTHTPGAGP DNSE**DEAGTEGPGAGETSAAHLQQTETKCDD PY*GPGYGRDE**HILTQAAETRIREGGFPCCSG *SQTPGREQDLPNC*DCRGKLPRGSTGGTLVRA FSCLSRGPAYEAFGD*R*LLGPHGACGY*GS*Q RKELVPSSLPCSWLLPLAQHGSLLCDERSGDR*D *ILCVGPVPG*DQPTAQLDGGRASAGHQG*AWS CGSCAPPSLCGSPRGPCGHIPVPNGPL*RGGDA PGEASQGGAASHSSGKPQLLPLGSPPENDP*CPA LHSRHLCGVALPPRPS*GSHLPPLPDPK*LLHS EGTGALLSKPWRRPAVLGVLRWPLGIRDQAASE RQER*DSGVGGLGETRRSHACNYSDPSSPHSRTF TSGCPAVAALCGPVSRAADSPSDIGGGCLGQTA WTGAEPGAVREGAG*EHEAQPDAEAVQLEPVLG PEVQRWTLPSPEGDPSSPHYGTLGEGQQKGTPAT QQLKYQHQPLTLESWLWLTLLWVSVSFSANKLP SGLPSSTKVMEL**CLCWALCVHARDATGGPSP GGLTASQGMSIWSWQDPCRPHRASSGGHSSQA*S PPDPIQAQRGIGGTWNHLPLAVSQGEPQNWGSA WEATWILGFVQEDLQEQANRVKWKEVYSENKGV SQLF*NLSSRLSSFYQKTPIN*KFFT*I*ELKKY KKEKMKIKE*EVTYS

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNKMAGGAWGRLACYLEFLKKEELKEFQL LLANKAHSRSSSGETPAQPEKTSGMEVASYLVAQ YGEQRAWDLALHTWEQMGLRSLCAQAQEGAGHS PSFPYSPSEPHLGSPSQPTSTAVLMPWIHELPA GCTQGSERRVLRQLPDTSGRRWREISASLLYQAL PSSPDHESPSQESPNAPTSTAVLGSWGSPPQPS LAPREQEAPGTQWPLDETSGIYYTEIREREREK SEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVR ESLCSTWPWKNEDFNQKFTQLLLLQRPHPRSQD PLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQE PRIVILQGAAGIGKSTLARQVKEAWGRGQLYGDR FQHVFYFSCRELAQSKVVSLAELIGKDGTATPA PIRQILSRPERLLFILDGVDEPGWVLQEPSSEL CLHWSQPQPADALLGSLLGKTILPEASFLITART TALQNLIPSLEQARWVEVLGFSESSRKEYFYRY FTDERQAIRAFRLVKSNKELWALCLVPWVSWLA CTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQ AQPLGPQLRDLCSLAAEGIWQKKTLFSPDDLRK HGLDGAIISTFLKMGILQEHPIPLSYSFIHLCF QEFFAAMSYVLEDEKGRGKHSNCIIDLEKTLEAY GIHGLFGASTTRFLLGLLSDEGEREMENIFHCR LSQGRNLMQWVPSLQLLLQPHSLESLHCLYETR NKTFLTQVMAHFEEMGMCVETDMELLVCTFCIKF SRHVKKLQLIEGRQHRSTWSPTMVVLFRWVPVT DAYWQILFSVLKVTRNLKELDLSGNSLSHSAVK SLCKTLRRPRCLLETLRLAGCGLTAEDCKDLAFG LRANQTLTELDLSFNVLTDAGAKHLCQRLRQPS CKLQRLQLVSCGLTSDCCQDLASVLSASPSLKE LDLQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQ TTLSDEMRQELRALEQEKPQLLIFSRRKPSVMT PTEGLDTGEMSNSTSSLKRQRLGSERAASHVAQ ANLKLLDVSKIFPIAEIAEESSPEVVPVELLCVP SPASQGDLHTKPLGTDDDFWGPTGPVATEVVDK EKNLYRVHFPVAGSYRWPNTGLCFVMREAVTVE IEFCVWDQFLGEINPQHSWMVAGPLLDIKAEPGA VEAVHLPHFVALQGGHVDTSLFQMAHFKEEGML LEKPARVELHHIVLENPSFSPLGVLLKMIHNAL RFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIR KELELCYRSPGEDQLFSEFYVGHLGSGIRLQVK DKKDETLVWEALVKPGDLMPATTLIPPARIAVP SPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLH GQVLSQEQYERVLAENTRPSQMRKLFSLSQSWD RKCKDGLYQALKETHPHLIMELWEKGSKKGLLP LSS*SINTSP*PLSPGFG*PFFGSQFLSLQTSCH LVCLPALK*WNFDDAFAGHYVSMPGMPQGAPVQ VA*QHLRECPSGAGKTPADLIEPHLVATAAKPR ALRIPSRRKEE*EGHGTICLWLCHRVSPKIGVQR GRPRGFLALYRKIYKSKPTE*SGRKFIQKIKEY HSSFRICLAGFPVFTRKPL*IKNFLLKFKN*KN TKKKK*K*RNKKLPTP

Sequence NALP1.eAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAGTGAGCGTTGCCCACACCCCATCTCCCGTCACCACATCTCCCCTCACCCTCACCCTCCCTGCCTGGCCCTGGACCCCATCC CAGGACCTCCCTATCAGCTGACTTCTTCCAGTGTCTTGCAGGCCCCTCTGGGCTCCTCCCTCCCCTGGCTTTTCCTACCACTCCCCCTCTATCGGCGTCT ATCTGTAGGTGCCCTGGGATTTATAAAACTGGGTTCCGAATGCTGAATAAGAGACGGTAAGAGCCAAGGCAAAGGACAGCACTGTTCTCTGCCTGCCTGA TACCCTCACCACCTGGGAACATCCCCCAGACACCCTCTTAACTCCGGGACAGAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCC TGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAG TGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTG TGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAG TGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAG AGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTG CTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACT ACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACA GCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTA CTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAG ACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGA AGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAG CTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAG GATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACT TCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCT GAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCC TGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAAC CCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAA AAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCC CTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTG CATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGG GAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTC TGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACAT GGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACC ATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAA GTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCT CACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCC AAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCT CTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCC TGCCTGCAAACTCATACGCCTGGGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGG CAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGG AAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTT CTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAAC ACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGA TGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACAC ATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTC TCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAG TCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTT CTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATG CCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCTGCACTTTGTGGACCAGTATCGAGAGCAGCTGA TAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCC CAGCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAAGGAGACCCATCCTCACCTCATT ATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGCTGAAGTATCAACACCAGCCCTTGACCCTTGAGTCCTGGCTTTGGCTGAC CCTTCTTTGGGTCTCAGTTTCTTTCTCTGCAAACAAGTTGCCATCTGGTTTGCCTTCCAGCACTAAAGTAATGGAACTTTGATGATGCCTTTGCTGGGCA TTATGTGTCCATGCCAGGGATGCCACAGGGGGCCCCAGTCCAGGTGGCCTAACAGCATCTCAGGGAATGTCCATCTGGAGCTGGCAAGACCCCTGCAGAC CTCATAGAGCCTCATCTGGTGGCCACAGCAGCCAAGCCTAGAGCCCTCCGGATCCCATCCAGGCGCAAAGAGGAATAGGAGGGACATGGAACCATTTGCC TCTGGCTGTGTCACAGGGTGAGCCCCAAAATTGGGGTTCAGCGTGGGAGGCCACGTGGATTCTTGGCTTTGTACAGGAAGATCTACAAGAGCAAGCCAAC AGAGTAAAGTGGAAGGAAGTTTATTCAGAAAATAAAGGAGTATCACAGCTCTTTTAGAATTTGTCTAGCAGGCTTTCCAGTTTTTACCAGAAAACCCCTA TAAATTAAAAATTTTTTACTTAAATTTAAGAATTAAAAAAATACAAAAAAGAAAAAATGAAAATAAAGGAATAAGAAGTTACCTACTCCAT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTVSVAHTPSPVTTSPLTLTLPAWPWTPS QDLPIS*LLPVSCRPLWAPPSPGFSYHSPSIGV YL*VPWDL*NWVPNAE*ETVRAKAKDSTVLCLPD TLTTWEHPPDTLLTPGQRWLAEPGAAWPVTWSS *RRRS*RSSSFCSPIKRTPGALRVRHPLSQRRR VAWRWPRTWWLSMGSSGPGT*PSIPGSRWG*GHC APKPRKGQATLPHSPTAQVNPTWGLPANPPPPQ C*CPGSMNCRRGAPRAQREGF*DSCLTHLDAAG EKSLPHSSTKLFQAPQTMSLQARSHPTPPHPQQC WGAGDPHLSPA*HPESRRLLGPNGLWMKRQEFT TQKSEKERERNQRKAGPHGQRW*ERPHRRTPAY SPTTTHGSLL*ERASVPHGPGKMRILTKNSHSCY FYKDLTPEAKIPWSREAGLIMWRRIEDI*LRSE TYLAQAWIPKNLA*SYCRGLLELGSQHWPGR*R KPGGEASCMGTASSMSSTSAAESWPSPRW*VSLS SSEKMGQPLRLPLDRSCLGQSGCSSSSMV*MSQ DGSCRSRVLSSVCTGASHSRRMHCWAVCWGKLY FPRHPS*SRLGPQLCRTSFLLWSRHVG*RSWGSL SPAGRNISTDISQMKGKQLEPLGWSNQTKSSGP CVLCPGCPGWPALA*CSR*SGRKNSH*LPRPPQ PSVYITLPRLSKLSHWDPSSETSALWLLRASGKK RPFSVQMTSGSMG*MGPSSPPS*RWVFFKSTPS L*ATASFTSVSKSSLQQCPMSWRMRRGEVNILI AS*IWKRR*KHMEYMACLGHQPHVSYWAC*VMRG RERWRTSFTAGCLRGGT*CSGSRPCSCCCSHTL WSPSTACTRLGTKRS*HK*WPISKKWACV*KQT WSS*CALSALNSAAT*RSFS*LRAGSTDQHGAPP W*SCSGGSQSQMPIGRFSSPSSRSPET*RSWT* VETR*ATLQ*RVFVRP*DALAASWRPCGWLAVA SQLRTARTLPLG*EPTRP*PSWT*ASMCSRMLEP NTFARD*DSRAASYSDCSWSAVASRLTAARTWP LCLVPAPA*RS*TCSRTTWMTLACDCSVRGSGI LPANSYAWGNQV**PLLRAWIRER*VIAHPHSSG RDSDQRGRLPMLLRLISNSWT*ARSSQLLRLQR KAPQR*YRWNSCACLLLPLKGTCIRSLWGLTMT SGAPRGLWLLR*LTKKRTCTEFTSL*LAPTAGPT RVSAL**EKR*PLRLNSVCGTSSWVRSTHSTAG WWQGLCWTSRLSLELWKLCTSLTLWLSKGAMWT HPCSKWPTLKRRGCSWRSQPGWSCIT*FWKTPAS PPWESS*K*SIMPCASFPSPLWCCFTTASILRK SPSTST*SQVTAPFGRNWSSAIEALEKTSCSRS STLATWDQGSGCK*KTRKMRLWCGRPW*NQEISC LQLL*SLQPA*PYLHLWMPRSCCTLWTSIESS* *PE*HRWRLSWTNCMDRC*ARSSTRGCWLRTRG PARCGSCSA*ASPGTGSAKMDSTKP*RRPILTSL WNSGRRAAKRDSCHSAAEVSTPALDP*VLALAD PSLGLSFFLCKQVAIWFAFQH*SNGTLMMPLLG IMCPCQGCHRGPQSRWPNSISGNVHLELARPLQT S*SLIWWPQQPSLEPSGSHPGAKRNRRDMEPFA SGCVTG*APKLGFSVGGHVDSWLCTGRSTRASQ QSKVEGSLFRK*RSITALLEFV*QAFQFLPENPY KLKIFYLNLRIKKIQKRKNENKGIRSYLLH

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQ*ALPTPHLPSPHLPSPSPSLPGPGPHP RTSLSADFFQCLAGPSGLLPPLAFPTTPPLSAS ICRCPGIYKTGFRMLNKRR*EPRQRTALFSACL IPSPPGNIPQTPS*LRDRDGWRSLGPPGLLLGVP EEGGAEGVPASARQ*SALQELFG*DTRSAREDE WHGGGLVPGGSVWGAAGLGPSPPYLGADGAEVT VRPSPGRGRPLSLIPLQPK*TPPGVSQPTHLHRS ANALDP*IAGGVHPGLREKGFETAA*HIWTPLE RNLCLTPLPSSSKLPRP*VSKPGVTQRPHIHSS AGELGIPTSAQPSTQRAGGSWDPMASG*NVRNLL HRNQRKREREIRERQAPMGSGGRNAPTGAHQPT APPPPMGAFCEREPLFHMALEK*GF*PKIHTAA TSTKTSPQKPRSPGQEKLA*LCGGESRTFN*DQR LIWPRPGYPRTSHSHTAGGCWNWEVNTGQAGEG SLGERPAVWGPLPACLLLQLQRAGPVQGGESR* AHRKRWDSHSGSH*TDPV*ARAAALHPRWCR*AR MGLAGAEF*ALSALEPATAGGCTAGQFAGENYT SRGILPDHGSDHSSAEPHSFFGAGTLGRGPGVL *VQQEGIFLQIFHR*KASN*SL*VGQIKQRALGP VSCALGVLAGLHLPDAADEAEGKTHTDFQDHHN PLSTLPCPGSPSSAIGTPAQRPLLSGC*GHLAK KDPFQSR*PQEAWVRWGHHLHLLEDGYSSRAPHP SELQLHSPLFPRVLCSNVLCLGG*EGER*TF*L HHRFGKDARSIWNTWPVWGINHTFPIGPVK**G GERDGEHLSLPAVSGEEPDAVGPVPAAAAAATLS GVPPLLVRDSEQNVPDTSDGPFRRNGHVCRNRH GALSVHFLH*IQPPREEASAD*GQAAQINMEPH HGSPVQVGPSHRCLLADSLLRPQGHQKPEGAGPK WKLAEPLCSEESL*DPETPSLPPGDPAVGWLWP HS*GLQGPCLWAESQPDPDRAGPELQCAHGCWS QTPLPETETAELQATATAAGQLWPHV*LLPGPGL CA*CQPQPEGARPAAEQPG*RWRATAL*GAQAS CLQTHTPGETKCDDPY*GPGYGRDE**HILTQA AETRIREGGFPCCSG*SQTPGREQDLPNC*DCRG KLPRGSTGGTLVRAFSCLSRGPAYEAFGD*R*L LGPHGACGY*GS*QRKELVPSSLPCSWLLPLAQ HGSLLCDERSGDR*D*ILCVGPVPG*DQPTAQLD GGRASAGHQG*AWSCGSCAPPSLCGSPRGPCGH IPVPNGPL*RGGDAPGEASQGGAASHSSGKPQL LPLGSPPENDP*CPALHSRHLCGVALPPRPS*GS HLPPLPDPK*LLHSEGTGALLSKPWRRPAVLGV LRWPLGIRDQAASERQER*DSGVGGLGETRRSH ACNYSDPSSPHSRTFTSGCPAVAALCGPVSRAAD SPSDIGGGCLGQTAWTGAEPGAVREGAG*EHEA QPDAEAVQLEPVLGPEVQRWTLPSPEGDPSSPH YGTLGEGQQKGTPATQQLKYQHQPLTLESWLWLT LLWVSVSFSANKLPSGLPSSTKVMEL**CLCWA LCVHARDATGGPSPGGLTASQGMSIWSWQDPCR PHRASSGGHSSQA*SPPDPIQAQRGIGGTWNHLP LAVSQGEPQNWGSAWEATWILGFVQEDLQEQAN RVKWKEVYSENKGVSQLF*NLSSRLSSFYQKTP IN*KFFT*I*ELKKYKKEKMKIKE*EVTYS

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNSERCPHPISRHHISPHPHPPCLALDPI PGPPYQLTSSSVLQAPLGSSLPWLFLPLPLYRRL SVGALGFIKLGSEC*IRDGKSQGKGQHCSLPA* YPHHLGTSPRHPLNSGTEMAGGAWGRLACYLEF LKKEELKEFQLLLANKAHSRSSSGETPAQPEKTS GMEVASYLVAQYGEQRAWDLALHTWEQMGLRSL CAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTA VLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWR EISASLLYQALPSSPDHESPSQESPNAPTSTAV LGSWGSPPQPSLAPREQEAPGTQWPLDETSGIY YTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQ PHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLL LLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIR DLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKE AWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAE LIGKDGTATPAPIRQILSRPERLLFILDGVDEP GWVLQEPSSELCLHWSQPQPADALLGSLLGKTIL PEASFLITARTTALQNLIPSLEQARWVEVLGFS ESSRKEYFYRYFTDERQAIRAFRLVKSNKELWA LCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTT LCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQK KTLFSPDDLRKHGLDGAIISTFLKMGILQEHPI PLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNC IIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHS LESLHCLYETRNKTFLTQVMAHFEEMGMCVETDM ELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPT MVVLFRWVPVTDAYWQILFSVLKVTRNLKELDL SGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGL TAEDCKDLAFGLRANQTLTELDLSFNVLTDAGA KHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLA SVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHP ACKLIRLGKPSVMTPTEGLDTGEMSNSTSSLKR QRLGSERAASHVAQANLKLLDVSKIFPIAEIAE ESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDF WGPTGPVATEVVDKEKNLYRVHFPVAGSYRWPN TGLCFVMREAVTVEIEFCVWDQFLGEINPQHSW MVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDT SLFQMAHFKEEGMLLEKPARVELHHIVLENPSF SPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEE VTFHLYLIPSDCSIRKELELCYRSPGEDQLFSEF YVGHLGSGIRLQVKDKKDETLVWEALVKPGDLM PATTLIPPARIAVPSPLDAPQLLHFVDQYREQL IARVTSVEVVLDKLHGQVLSQEQYERVLAENTRP SQMRKLFSLSQSWDRKCKDGLYQALKETHPHLI MELWEKGSKKGLLPLSS*SINTSP*PLSPGFG* PFFGSQFLSLQTSCHLVCLPALK*WNFDDAFAGH YVSMPGMPQGAPVQVA*QHLRECPSGAGKTPAD LIEPHLVATAAKPRALRIPSRRKEE*EGHGTIC LWLCHRVSPKIGVQRGRPRGFLALYRKIYKSKPT E*SGRKFIQKIKEYHSSFRICLAGFPVFTRKPL *IKNFLLKFKN*KNTKKKK*K*RNKKLPTP

Sequence NALP1.oAug05

TTCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGG AAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGA GTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAAGGAAC ACCAGCCAGCCGTGGAACCTCAGGTGCAACAGAGACGCCAGGAGATACTAGTGCCCAGCAGCCTGCGGCAGTACCAATGAAGCCAGAGAGGGCTTGGTGG ATGACAAGGAGGCCTGAGTAGACCGCAGGTGGGTCTGAGAAATGGGCTTAGGTGAGGCAGGTCTTTGAAGGATTTGTTCTTAATCATATGCGAGATGCTC AAAAGGCTGGATGCCTGCTTTTGTGGGTGAAGAGCAAGAAGAGAAAACAGGTTGTACACATACAGATGCAGATGGAGAGACAGAGAAAAAAAAGGAAGAA GGCAGAGAAATGCACCAATTCTTGAGCTGTATTATCTCTGGACCTTGGGATTGTGGGAGGCTTTATTTTACTACTGATTTTGCCTACACTGTTTTCTCAA TTTCTAGTTTTCTACAAAGATGATGTGTTAGCTTTTTCACGCATTAAGATTAAAATTTAAAACAGACCACACAATAAATATTTCTTTTAAAAGGAGAAAT ATGCCCTGGGGAAGCTCCCCACGCAGCTGAGAGCCTGGCTGGTTGCATTTCGGCTTCTTAGGTGCCAAGCCTACTAGTGCCCCGGGGTCCCAGCCAGCGG CTCGCGTTTCCTCCCCCTTGCGCCGCCAGTCTGGTTGCCATGGAGACAAACCTTGCTGCGCAGAGACTGCCGAGCCGCAGGCAGCGGAGCTGGAACCCGG CGAGCCAGCCTCTGCAACAGGCTGGGGGCGGAAGGAGGAGCCAGGCGAAGCGGCGCCTCAGCTGAGAGGACCGGCGGACCCTGCAGAGGCCCCCTGCCCC TCTGGCTCCGCCCCCACCCGGGTCGCTAGAAATACAGCCGTAGCCCCGCCCACCGCCCACTGCGCTCTGACCCAGACCCGGCTGACCCACCTACCCGCGA TCCTGCCCATGGCTGACGGGCTCTTTCGGCGCAGACCCTGGGGTCTCGAGCAGATTCGCCCGGACCCCGAGTCCGAAGGCCTGTTTGACAAGCCTCCCCC GGAAGACCCTCCCGCTGCCCGCGGGCCCAGGTCGGCGTCGGCCGCGGGCAAGAAGGCTGGTCGGCGCGCGGGCGGGAGGGCGCAGGGGGGCCGCGCCGGG CAGCCCCCGAAGGCCGCATCGCGCCCCCCGCCCAAGAAGGAGGCGCCTCCACTGGACGAGGGCTGCTATCTCGACCATTTTCCGCACCTCTCCATCTTCA TCTACGCAGCCATCGCCTTCTCCATCACCTCCTGCATCTTTACCTATATCCATTTACAGCTTGCCTGAGTGGCCAGCGCGGGACGGGGTGGGCGCAGGAC CGAGCGGGGAGGGAAAGGGAAAACGGGGCTCGGCATTTTGTGTTTTAGAACAGCGCTGCACCCCCTTCATGTAGCTTTCGATGCTTGTTTCTTTCCGTCT TTGTTGTCACTATCTTTGTCTATCAGTACGAAAGTACAAAGTAGCTGCCGGCAATGAAATAGGGGTGCTGTTTGCACCTGCAGGTTAGGGGTGGAGGCGT TTAGAATTTTGGGGTGTGATTGAGCCCCGTTTATAATTAGAATGCCCCTGGACCCCTACCACTCTGTGACGTGGGGGCACGCGCAGGGATCCCATCATTT TGTGTTTGGGGAGCTCAGAGTGCGCCCAATCTTGGAATCTTTAAGGGATGAGCCAGACCCAGACCCGCGGCCTTCTAGAGAGGGTCCGGCAGGGAGGGTC GGCGCCCTGGCCCGGGGTGGGCCGGAGCCCTGTGATGCTGCATCGCCCCCAGGAGGAGCCAGCTGTGCCCCAGAGTTGGCGCGGCCGAGAGAGGACAAGA GCGCGCAGCAGGCGAAGCTGGAGGGCGGGACTCGGTAAGTGGCGTTCGTCGGGGTGTCGTGCTGCGCCCCCAGGGGCTCCGGCTGACCACGACTGTGTGT TTTTCCTGCCTTAGACTTTGTTGTCGCTGCCCGGAGGAGTCGAGACTGGTACCCGGAGGAGCTGTCTCACCAGGAGACCACGTCCTGGAAGTGTCCGGGA CTCGCGGGACCTGTGGCTGCAGACCCCGCCGGCACGCAGGCCCAGAGCTGGCGCACTCCTGAGGATGAGACTCTGGGGGCCCTAGCCGGGGTCCACGGGA GGGCTGTCCTTGGGGACTCTAGGATGGCTTCGTTCTGGCCCGGCTCACTTCTGGAGCTGTGAGACCCAAGACAAAAGGGGCTGAGGGATTTCTCATTGAC AAGAGTTCGTGCGGGAAAACCACCTGATCCCTAGGGATTTGTCATCTTAAGACTCAAAAGGCTTAATACCAGGAACCACCTTGGCAAGATATTTACCCAC CGGCCATCTCTGTTTACTCATGAATGTTAAATGTTAAAACGCAGCGCTCTAACCCTGCATATTATTTACTTGCAAATGTCTGTAATCTGTAATTGTGATG CCTCTGATGGAATAAATTATCTTTTTCAGTCTCCTCT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

FSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPE EVTFHLYLIPSDCSIRKELELCYRSPGEDQLFSE FYVGHLGSGIRLQVKDKKDETLVWEALVKPGRN TSQPWNLRCNRDARRY*CPAACGSTNEAREGLV DDKEA*VDRRWV*EMGLGEAGL*RICS*SYARCS KGWMPAFVGEEQEEKTGCTHTDADGETEKKKEE GREMHQFLSCIISGPWDCGRLYFTTDFAYTVFS ISSFLQR*CVSFFTH*D*NLKQTTQ*IFLLKGEI CPGEAPHAAESLAGCISAS*VPSLLVPRGPSQR LAFPPPCAASLVAMETNLAAQRLPSRRQRSWNP ASQPLQQAGGGRRSQAKRRLS*EDRRTLQRPPAP LAPPPPGSLEIQP*PRPPPTAL*PRPG*PTYPR SCPWLTGSFGADPGVSSRFARTPSPKACLTSLP RKTLPLPAGPGRRRPRARRLVGARAGGRRGAAPG SPRRPHRAPRPRRRRLHWTRAAISTIFRTSPSS STQPSPSPSPPASLPISIYSLPEWPARDGVGAG PSGEGKGKRGSAFCVLEQRCTPFM*LSMLVSFRL CCHYLCLSVRKYKVAAGNEIGVLFAPAG*GWRR LEFWGVIEPRL*LECPWTPTTL*RGGTRRDPII LCLGSSECAQSWNL*GMSQTQTRGLLERVRQGGS APWPGVGRSPVMLHRPQEEPAVPQSWRGRERTR ARSRRSWRAGLGKWRSSGCRAAPPGAPADHDCV FFLP*TLLSLPGGVETGTRRSCLTRRPRPGSVRD SRDLWLQTPPARRPRAGALLRMRLWGP*PGSTG GLSLGTLGWLRSGPAHFWSCETQDKRG*GISH* QEFVRENHLIPRDLSS*DSKGLIPGTTLARYLPT GHLCLLMNVKC*NAAL*PCILFTCKCL*SVIVM PLME*IIFFSLL

Frame 1

SPPWESS*K*SIMPCASFPSPLWCCFTTASILR KSPSTST*SQVTAPFGRNWSSAIEALEKTSCSR SSTLATWDQGSGCK*KTRKMRLWCGRPW*NQEGT PASRGTSGATETPGDTSAQQPAAVPMKPERAWW MTRRPE*TAGGSEKWA*VRQVFEGFVLNHMRDA QKAGCLLLWVKSKKRKQVVHIQMQMERQRKKRKK AEKCTNS*AVLSLDLGIVGGFILLLILPTLFSQ FLVFYKDDVLAFSRIKIKI*NRPHNKYFF*KEK YALGKLPTQLRAWLVAFRLLRCQAY*CPGVPASG SRFLPLAPPVWLPWRQTLLRRDCRAAGSGAGTR RASLCNRLGAEGGARRSGASAERTGGPCRGPLP LWLRPHPGR*KYSRSPAHRPLRSDPDPADPPTRD PAHG*RALSAQTLGSRADSPGPRVRRPV*QASP GRPSRCPRAQVGVGRGQEGWSARGREGAGGPRR AAPEGRIAPPAQEGGASTGRGLLSRPFSAPLHLH LRSHRLLHHLLHLYLYPFTACLSGQRGTGWAQD RAGRERENGARHFVF*NSAAPPSCSFRCLFLSV FVVTIFVYQYESTK*LPAMK*GCCLHLQVRGGGV *NFGV*LSPVYN*NAPGPLPLCDVGARAGIPSF CVWGAQSAPNLGIFKG*ARPRPAAF*RGSGREG RRPGPGWAGAL*CCIAPRRSQLCPRVGAAERGQE RAAGEAGGRDSVSGVRRGVVLRPQGLRLTTTVC FSCLRLCCRCPEESRLVPGGAVSPGDHVLEVSG TRGTCGCRPRRHAGPELAHS*G*DSGGPSRGPRE GCPWGL*DGFVLARLTSGAVRPKTKGAEGFLID KSSCGKTT*SLGICHLKTQKA*YQEPPWQDIYP PAISVYS*MLNVKTQRSNPAYYLLANVCNL*L*C L*WNKLSFSVSS

Frame 2

LPLGSPPENDP*CPALHSRHLCGVALPPRPS*G SHLPPLPDPK*LLHSEGTGALLSKPWRRPAVLG VLRWPLGIRDQAASERQER*DSGVGGLGETRKE HQPAVEPQVQQRRQEILVPSSLRQYQ*SQRGLGG *QGGLSRPQVGLRNGLR*GRSLKDLFLIICEML KRLDACFCG*RARRENRLYTYRCRWRDREKKGR RQRNAPILELYYLWTLGLWEALFYY*FCLHCFLN F*FSTKMMC*LFHALRLKFKTDHTINISFKRRN MPWGSSPRS*EPGWLHFGFLGAKPTSAPGSQPA ARVSSPLRRQSGCHGDKPCCAETAEPQAAELEPG EPASATGWGRKEEPGEAAPQLRGPADPAEAPCP SGSAPTRVARNTAVAPPTAHCALTQTRLTHLPA ILPMADGLFRRRPWGLEQIRPDPESEGLFDKPPP EDPPAARGPRSASAAGKKAGRRAGGRAQGGRAG QPPKAASRPPPKKEAPPLDEGCYLDHFPHLSIF IYAAIAFSITSCIFTYIHLQLA*VASAGRGGRRT ERGGKGKTGLGILCFRTALHPLHVAFDACFFPS LLSLSLSISTKVQSSCRQ*NRGAVCTCRLGVEA FRILGCD*APFIIRMPLDPYHSVTWGHAQGSHHF VFGELRVRPILESLRDEPDPDPRPSREGPAGRV GALARGGPEPCDAASPPGGASCAPELARPREDK SAQQAKLEGGTR*VAFVGVSCCAPRGSG*PRLCV FPALDFVVAARRSRDWYPEELSHQETTSWKCPG LAGPVAADPAGTQAQSWRTPEDETLGALAGVHG RAVLGDSRMASFWPGSLLEL*DPRQKGLRDFSLT RVRAGKPPDP*GFVILRLKRLNTRNHLGKIFTH RPSLFTHEC*MLKRSALTLHIIYLQMSVICNCD ASDGINYLFQSP

Sequence NALP1.aAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAGTGAGCGTTGCCCACACCCCATCTCCCGTCACCACATCTCCCCTCACCCTCACCCTCCCTGCCTGGCCCTGGACCCCATCC CAGGACCTCCCTATCAGCTGACTTCTTCCAGTGTCTTGCAGGCCCCTCTGGGCTCCTCCCTCCCCTGGCTTTTCCTACCACTCCCCCTCTATCGGCGTCT ATCTGTAGGTGCCCTGGGATTTATAAAACTGGGTTCCGAATGCTGAATAAGAGACGGTAAGAGCCAAGGCAAAGGACAGCACTGTTCTCTGCCTGCCTGA TACCCTCACCACCTGGGAACATCCCCCAGACACCCTCTTAACTCCGGGACAGAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCC TGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAG TGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTG TGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAG TGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAG AGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTG CTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACT ACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACA GCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTA CTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAG ACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGA AGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAG CTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAG GATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACT TCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCT GAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCC TGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAAC CCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAA AAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCC CTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTG CATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGG GAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTC TGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACAT GGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACC ATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAA GTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCT CACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCC AAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCT CTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCC TGCCTGCAAACTCATACGCCTGGGGCTGGACCAGACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTC ATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCG GATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCC AGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGGGGCCCC ACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCT GCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGG GCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTC CAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGG GAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCA CCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCACCCCCGCTGACC CCACTTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAG AAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTT GGTGAAACCAGGAGATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCTGCACTTTGTG GACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGG TGCTGGCTGAGAACACGAGGCCCAGCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAA GGAGACCCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGCTGAAGTATCAACACCAGCCCTTGACCC TTGAGTCCTGGCTTTGGCTGACCCTTCTTTGGGTCTCAGTTTCTTTCTCTGCAAACAAGTTGCCATCTGGTTTGCCTTCCAGCACTAAAGTAATGGAACT TTGATGATGCCTTTGCTGGGCATTATGTGTCCATGCCAGGGATGCCACAGGGGGCCCCAGTCCAGGTGGCCTAACAGCATCTCAGGGAATGTCCATCTGG AGCTGGCAAGACCCCTGCAGACCTCATAGAGCCTCATCTGGTGGCCACAGCAGCCAAGCCTAGAGCCCTCCGGATCCCATCCAGGCGCAAAGAGGAATAG GAGGGACATGGAACCATTTGCCTCTGGCTGTGTCACAGGGTGAGCCCCAAAATTGGGGTTCAGCGTGGGAGGCCACGTGGATTCTTGGCTTTGTACAGGA AGATCTACAAGAGCAAGCCAACAGAGTAAAGTGGAAGGAAGTTTATTCAGAAAATAAAGGAGTATCACAGCTCTTTTAGAATTTGTCTAGCAGGCTTTCC AGTTTTTACCAGAAAACCCCTATAAATTAAAAATTTTTTACTTAAATTTAAGAATTAAAAAAATACAAAAAAGAAAAAATGAAAATAAAGGAATAAGAAG TTACCTACTCCAT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTVSVAHTPSPVTTSPLTLTLPAWPWTPS QDLPIS*LLPVSCRPLWAPPSPGFSYHSPSIGV YL*VPWDL*NWVPNAE*ETVRAKAKDSTVLCLPD TLTTWEHPPDTLLTPGQRWLAEPGAAWPVTWSS *RRRS*RSSSFCSPIKRTPGALRVRHPLSQRRR VAWRWPRTWWLSMGSSGPGT*PSIPGSRWG*GHC APKPRKGQATLPHSPTAQVNPTWGLPANPPPPQ C*CPGSMNCRRGAPRAQREGF*DSCLTHLDAAG EKSLPHSSTKLFQAPQTMSLQARSHPTPPHPQQC WGAGDPHLSPA*HPESRRLLGPNGLWMKRQEFT TQKSEKERERNQRKAGPHGQRW*ERPHRRTPAY SPTTTHGSLL*ERASVPHGPGKMRILTKNSHSCY FYKDLTPEAKIPWSREAGLIMWRRIEDI*LRSE TYLAQAWIPKNLA*SYCRGLLELGSQHWPGR*R KPGGEASCMGTASSMSSTSAAESWPSPRW*VSLS SSEKMGQPLRLPLDRSCLGQSGCSSSSMV*MSQ DGSCRSRVLSSVCTGASHSRRMHCWAVCWGKLY FPRHPS*SRLGPQLCRTSFLLWSRHVG*RSWGSL SPAGRNISTDISQMKGKQLEPLGWSNQTKSSGP CVLCPGCPGWPALA*CSR*SGRKNSH*LPRPPQ PSVYITLPRLSKLSHWDPSSETSALWLLRASGKK RPFSVQMTSGSMG*MGPSSPPS*RWVFFKSTPS L*ATASFTSVSKSSLQQCPMSWRMRRGEVNILI AS*IWKRR*KHMEYMACLGHQPHVSYWAC*VMRG RERWRTSFTAGCLRGGT*CSGSRPCSCCCSHTL WSPSTACTRLGTKRS*HK*WPISKKWACV*KQT WSS*CALSALNSAAT*RSFS*LRAGSTDQHGAPP W*SCSGGSQSQMPIGRFSSPSSRSPET*RSWT* VETR*ATLQ*RVFVRP*DALAASWRPCGWLAVA SQLRTARTLPLG*EPTRP*PSWT*ASMCSRMLEP NTFARD*DSRAASYSDCSWSAVASRLTAARTWP LCLVPAPA*RS*TCSRTTWMTLACDCSVRGSGI LPANSYAWGWTRQL*VMR*GRN*GPWSRRNLSCS SSADGNQV**PLLRAWIRER*VIAHPHSSGRDS DQRGRLPMLLRLISNSWT*ARSSQLLRLQRKAP QR*YRWNSCACLLLPLKGTCIRSLWGLTMTSGAP RGLWLLR*LTKKRTCTEFTSL*LAPTAGPTRVS AL**EKR*PLRLNSVCGTSSWVRSTHSTAGWWQ GLCWTSRLSLELWKLCTSLTLWLSKGAMWTHPCS KWPTLKRRGCSWRSQPGWSCIT*FWKTPASPPW ESS*K*SIMPCASFPSPLWCCFTTASILRKSPS TST*SQVTAPFGRP*MI*K*NSSLCESTSHPR*P HFIWAVVTLCLGLVQGCWKYSPRNWSSAIEALE KTSCSRSSTLATWDQGSGCK*KTRKMRLWCGRP W*NQEISCLQLL*SLQPA*PYLHLWMPRSCCTLW TSIESS**PE*HRWRLSWTNCMDRC*ARSSTRG CWLRTRGPARCGSCSA*ASPGTGSAKMDSTKP* RRPILTSLWNSGRRAAKRDSCHSAAEVSTPALDP *VLALADPSLGLSFFLCKQVAIWFAFQH*SNGT LMMPLLGIMCPCQGCHRGPQSRWPNSISGNVHL ELARPLQTS*SLIWWPQQPSLEPSGSHPGAKRNR RDMEPFASGCVTG*APKLGFSVGGHVDSWLCTG RSTRASQQSKVEGSLFRK*RSITALLEFV*QAF QFLPENPYKLKIFYLNLRIKKIQKRKNENKGIRS YLLH

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQ*ALPTPHLPSPHLPSPSPSLPGPGPHP RTSLSADFFQCLAGPSGLLPPLAFPTTPPLSAS ICRCPGIYKTGFRMLNKRR*EPRQRTALFSACL IPSPPGNIPQTPS*LRDRDGWRSLGPPGLLLGVP EEGGAEGVPASARQ*SALQELFG*DTRSAREDE WHGGGLVPGGSVWGAAGLGPSPPYLGADGAEVT VRPSPGRGRPLSLIPLQPK*TPPGVSQPTHLHRS ANALDP*IAGGVHPGLREKGFETAA*HIWTPLE RNLCLTPLPSSSKLPRP*VSKPGVTQRPHIHSS AGELGIPTSAQPSTQRAGGSWDPMASG*NVRNLL HRNQRKREREIRERQAPMGSGGRNAPTGAHQPT APPPPMGAFCEREPLFHMALEK*GF*PKIHTAA TSTKTSPQKPRSPGQEKLA*LCGGESRTFN*DQR LIWPRPGYPRTSHSHTAGGCWNWEVNTGQAGEG SLGERPAVWGPLPACLLLQLQRAGPVQGGESR* AHRKRWDSHSGSH*TDPV*ARAAALHPRWCR*AR MGLAGAEF*ALSALEPATAGGCTAGQFAGENYT SRGILPDHGSDHSSAEPHSFFGAGTLGRGPGVL *VQQEGIFLQIFHR*KASN*SL*VGQIKQRALGP VSCALGVLAGLHLPDAADEAEGKTHTDFQDHHN PLSTLPCPGSPSSAIGTPAQRPLLSGC*GHLAK KDPFQSR*PQEAWVRWGHHLHLLEDGYSSRAPHP SELQLHSPLFPRVLCSNVLCLGG*EGER*TF*L HHRFGKDARSIWNTWPVWGINHTFPIGPVK**G GERDGEHLSLPAVSGEEPDAVGPVPAAAAAATLS GVPPLLVRDSEQNVPDTSDGPFRRNGHVCRNRH GALSVHFLH*IQPPREEASAD*GQAAQINMEPH HGSPVQVGPSHRCLLADSLLRPQGHQKPEGAGPK WKLAEPLCSEESL*DPETPSLPPGDPAVGWLWP HS*GLQGPCLWAESQPDPDRAGPELQCAHGCWS QTPLPETETAELQATATAAGQLWPHV*LLPGPGL CA*CQPQPEGARPAAEQPG*RWRATAL*GAQAS CLQTHTPGAGPDNSE**DEAGTEGPGAGETSAA HLQQTETKCDDPY*GPGYGRDE**HILTQAAETR IREGGFPCCSG*SQTPGREQDLPNC*DCRGKLP RGSTGGTLVRAFSCLSRGPAYEAFGD*R*LLGP HGACGY*GS*QRKELVPSSLPCSWLLPLAQHGSL LCDERSGDR*D*ILCVGPVPG*DQPTAQLDGGR ASAGHQG*AWSCGSCAPPSLCGSPRGPCGHIPV PNGPL*RGGDAPGEASQGGAASHSSGKPQLLPLG SPPENDP*CPALHSRHLCGVALPPRPS*GSHLP PLPDPK*LLHSEGHR*SRNEIPVCANPQATPAD PTLYGLSLHCVWVWFRDAGNTPQGTGALLSKPWR RPAVLGVLRWPLGIRDQAASERQER*DSGVGGL GETRRSHACNYSDPSSPHSRTFTSGCPAVAALC GPVSRAADSPSDIGGGCLGQTAWTGAEPGAVREG AG*EHEAQPDAEAVQLEPVLGPEVQRWTLPSPE GDPSSPHYGTLGEGQQKGTPATQQLKYQHQPLT LESWLWLTLLWVSVSFSANKLPSGLPSSTKVMEL **CLCWALCVHARDATGGPSPGGLTASQGMSIW SWQDPCRPHRASSGGHSSQA*SPPDPIQAQRGI GGTWNHLPLAVSQGEPQNWGSAWEATWILGFVQE DLQEQANRVKWKEVYSENKGVSQLF*NLSSRLS SFYQKTPIN*KFFT*I*ELKKYKKEKMKIKE*E VTYS

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNSERCPHPISRHHISPHPHPPCLALDPI PGPPYQLTSSSVLQAPLGSSLPWLFLPLPLYRRL SVGALGFIKLGSEC*IRDGKSQGKGQHCSLPA* YPHHLGTSPRHPLNSGTEMAGGAWGRLACYLEF LKKEELKEFQLLLANKAHSRSSSGETPAQPEKTS GMEVASYLVAQYGEQRAWDLALHTWEQMGLRSL CAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTA VLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWR EISASLLYQALPSSPDHESPSQESPNAPTSTAV LGSWGSPPQPSLAPREQEAPGTQWPLDETSGIY YTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQ PHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLL LLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIR DLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKE AWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAE LIGKDGTATPAPIRQILSRPERLLFILDGVDEP GWVLQEPSSELCLHWSQPQPADALLGSLLGKTIL PEASFLITARTTALQNLIPSLEQARWVEVLGFS ESSRKEYFYRYFTDERQAIRAFRLVKSNKELWA LCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTT LCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQK KTLFSPDDLRKHGLDGAIISTFLKMGILQEHPI PLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNC IIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHS LESLHCLYETRNKTFLTQVMAHFEEMGMCVETDM ELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPT MVVLFRWVPVTDAYWQILFSVLKVTRNLKELDL SGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGL TAEDCKDLAFGLRANQTLTELDLSFNVLTDAGA KHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLA SVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHP ACKLIRLGLDQTTLSDEMRQELRALEQEKPQLL IFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRL GSERAASHVAQANLKLLDVSKIFPIAEIAEESSP EVVPVELLCVPSPASQGDLHTKPLGTDDDFWGP TGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGL CFVMREAVTVEIEFCVWDQFLGEINPQHSWMVAG PLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLF QMAHFKEEGMLLEKPARVELHHIVLENPSFSPL GVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFH LYLIPSDCSIRKAIDDLEMKFQFVRIHKPPPLT PLYMGCRYTVSGSGSGMLEILPKELELCYRSPG EDQLFSEFYVGHLGSGIRLQVKDKKDETLVWEAL VKPGDLMPATTLIPPARIAVPSPLDAPQLLHFV DQYREQLIARVTSVEVVLDKLHGQVLSQEQYER VLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALK ETHPHLIMELWEKGSKKGLLPLSS*SINTSP*P LSPGFG*PFFGSQFLSLQTSCHLVCLPALK*WN FDDAFAGHYVSMPGMPQGAPVQVA*QHLRECPSG AGKTPADLIEPHLVATAAKPRALRIPSRRKEE* EGHGTICLWLCHRVSPKIGVQRGRPRGFLALYR KIYKSKPTE*SGRKFIQKIKEYHSSFRICLAGFP VFTRKPL*IKNFLLKFKN*KNTKKKK*K*RNKK LPTP

Sequence NALP1.bAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAGTGAGCGTTGCCCACACCCCATCTCCCGTCACCACATCTCCCCTCACCCTCACCCTCCCTGCCTGGCCCTGGACCCCATCC CAGGACCTCCCTATCAGCTGACTTCTTCCAGTGTCTTGCAGGCCCCTCTGGGCTCCTCCCTCCCCTGGCTTTTCCTACCACTCCCCCTCTATCGGCGTCT ATCTGTAGGTGCCCTGGGATTTATAAAACTGGGTTCCGAATGCTGAATAAGAGACGGTAAGAGCCAAGGCAAAGGACAGCACTGTTCTCTGCCTGCCTGA TACCCTCACCACCTGGGAACATCCCCCAGACACCCTCTTAACTCCGGGACAGAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCC TGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAG TGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTG TGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAG TGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAG AGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTG CTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACT ACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACA GCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTA CTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAG ACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGA AGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAG CTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAG GATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACT TCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCT GAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCC TGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAAC CCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAA AAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCC CTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTG CATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGG GAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTC TGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACAT GGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACC ATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAA GTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCT CACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCC AAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCT CTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCC TGCCTGCAAACTCATACGCCTGGGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGG CAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGG AAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTT CTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAAC ACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGA TGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACAC ATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTC TCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAG TCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCACC CCCGCTGACCCCACTTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTATCGA AGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGT GGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCT GCACTTTGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAG TACGAGAGGGTGCTGGCTGAGAACACGAGGCCCAGCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACC AAGCCCTGAAGGAGACCCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGCTGAAGTATCAACACCAG CCCTTGACCCTTGAGTCCTGGCTTTGGCTGACCCTTCTTTGGGTCTCAGTTTCTTTCTCTGCAAACAAGTTGCCATCTGGTTTGCCTTCCAGCACTAAAG TAATGGAACTTTGATGATGCCTTTGCTGGGCATTATGTGTCCATGCCAGGGATGCCACAGGGGGCCCCAGTCCAGGTGGCCTAACAGCATCTCAGGGAAT GTCCATCTGGAGCTGGCAAGACCCCTGCAGACCTCATAGAGCCTCATCTGGTGGCCACAGCAGCCAAGCCTAGAGCCCTCCGGATCCCATCCAGGCGCAA AGAGGAATAGGAGGGACATGGAACCATTTGCCTCTGGCTGTGTCACAGGGTGAGCCCCAAAATTGGGGTTCAGCGTGGGAGGCCACGTGGATTCTTGGCT TTGTACAGGAAGATCTACAAGAGCAAGCCAACAGAGTAAAGTGGAAGGAAGTTTATTCAGAAAATAAAGGAGTATCACAGCTCTTTTAGAATTTGTCTAG CAGGCTTTCCAGTTTTTACCAGAAAACCCCTATAAATTAAAAATTTTTTACTTAAATTTAAGAATTAAAAAAATACAAAAAAGAAAAAATGAAAATAAAG GAATAAGAAGTTACCTACTCCAT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTVSVAHTPSPVTTSPLTLTLPAWPWTPS QDLPIS*LLPVSCRPLWAPPSPGFSYHSPSIGV YL*VPWDL*NWVPNAE*ETVRAKAKDSTVLCLPD TLTTWEHPPDTLLTPGQRWLAEPGAAWPVTWSS *RRRS*RSSSFCSPIKRTPGALRVRHPLSQRRR VAWRWPRTWWLSMGSSGPGT*PSIPGSRWG*GHC APKPRKGQATLPHSPTAQVNPTWGLPANPPPPQ C*CPGSMNCRRGAPRAQREGF*DSCLTHLDAAG EKSLPHSSTKLFQAPQTMSLQARSHPTPPHPQQC WGAGDPHLSPA*HPESRRLLGPNGLWMKRQEFT TQKSEKERERNQRKAGPHGQRW*ERPHRRTPAY SPTTTHGSLL*ERASVPHGPGKMRILTKNSHSCY FYKDLTPEAKIPWSREAGLIMWRRIEDI*LRSE TYLAQAWIPKNLA*SYCRGLLELGSQHWPGR*R KPGGEASCMGTASSMSSTSAAESWPSPRW*VSLS SSEKMGQPLRLPLDRSCLGQSGCSSSSMV*MSQ DGSCRSRVLSSVCTGASHSRRMHCWAVCWGKLY FPRHPS*SRLGPQLCRTSFLLWSRHVG*RSWGSL SPAGRNISTDISQMKGKQLEPLGWSNQTKSSGP CVLCPGCPGWPALA*CSR*SGRKNSH*LPRPPQ PSVYITLPRLSKLSHWDPSSETSALWLLRASGKK RPFSVQMTSGSMG*MGPSSPPS*RWVFFKSTPS L*ATASFTSVSKSSLQQCPMSWRMRRGEVNILI AS*IWKRR*KHMEYMACLGHQPHVSYWAC*VMRG RERWRTSFTAGCLRGGT*CSGSRPCSCCCSHTL WSPSTACTRLGTKRS*HK*WPISKKWACV*KQT WSS*CALSALNSAAT*RSFS*LRAGSTDQHGAPP W*SCSGGSQSQMPIGRFSSPSSRSPET*RSWT* VETR*ATLQ*RVFVRP*DALAASWRPCGWLAVA SQLRTARTLPLG*EPTRP*PSWT*ASMCSRMLEP NTFARD*DSRAASYSDCSWSAVASRLTAARTWP LCLVPAPA*RS*TCSRTTWMTLACDCSVRGSGI LPANSYAWGNQV**PLLRAWIRER*VIAHPHSSG RDSDQRGRLPMLLRLISNSWT*ARSSQLLRLQR KAPQR*YRWNSCACLLLPLKGTCIRSLWGLTMT SGAPRGLWLLR*LTKKRTCTEFTSL*LAPTAGPT RVSAL**EKR*PLRLNSVCGTSSWVRSTHSTAG WWQGLCWTSRLSLELWKLCTSLTLWLSKGAMWT HPCSKWPTLKRRGCSWRSQPGWSCIT*FWKTPAS PPWESS*K*SIMPCASFPSPLWCCFTTASILRK SPSTST*SQVTAPFGRP*MI*K*NSSLCESTSH PR*PHFIWAVVTLCLGLVQGCWKYSPRNWSSAIE ALEKTSCSRSSTLATWDQGSGCK*KTRKMRLWC GRPW*NQEISCLQLL*SLQPA*PYLHLWMPRSC CTLWTSIESS**PE*HRWRLSWTNCMDRC*ARSS TRGCWLRTRGPARCGSCSA*ASPGTGSAKMDST KP*RRPILTSLWNSGRRAAKRDSCHSAAEVSTP ALDP*VLALADPSLGLSFFLCKQVAIWFAFQH*S NGTLMMPLLGIMCPCQGCHRGPQSRWPNSISGN VHLELARPLQTS*SLIWWPQQPSLEPSGSHPGA KRNRRDMEPFASGCVTG*APKLGFSVGGHVDSWL CTGRSTRASQQSKVEGSLFRK*RSITALLEFV* QAFQFLPENPYKLKIFYLNLRIKKIQKRKNENK GIRSYLLH

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQ*ALPTPHLPSPHLPSPSPSLPGPGPHP RTSLSADFFQCLAGPSGLLPPLAFPTTPPLSAS ICRCPGIYKTGFRMLNKRR*EPRQRTALFSACL IPSPPGNIPQTPS*LRDRDGWRSLGPPGLLLGVP EEGGAEGVPASARQ*SALQELFG*DTRSAREDE WHGGGLVPGGSVWGAAGLGPSPPYLGADGAEVT VRPSPGRGRPLSLIPLQPK*TPPGVSQPTHLHRS ANALDP*IAGGVHPGLREKGFETAA*HIWTPLE RNLCLTPLPSSSKLPRP*VSKPGVTQRPHIHSS AGELGIPTSAQPSTQRAGGSWDPMASG*NVRNLL HRNQRKREREIRERQAPMGSGGRNAPTGAHQPT APPPPMGAFCEREPLFHMALEK*GF*PKIHTAA TSTKTSPQKPRSPGQEKLA*LCGGESRTFN*DQR LIWPRPGYPRTSHSHTAGGCWNWEVNTGQAGEG SLGERPAVWGPLPACLLLQLQRAGPVQGGESR* AHRKRWDSHSGSH*TDPV*ARAAALHPRWCR*AR MGLAGAEF*ALSALEPATAGGCTAGQFAGENYT SRGILPDHGSDHSSAEPHSFFGAGTLGRGPGVL *VQQEGIFLQIFHR*KASN*SL*VGQIKQRALGP VSCALGVLAGLHLPDAADEAEGKTHTDFQDHHN PLSTLPCPGSPSSAIGTPAQRPLLSGC*GHLAK KDPFQSR*PQEAWVRWGHHLHLLEDGYSSRAPHP SELQLHSPLFPRVLCSNVLCLGG*EGER*TF*L HHRFGKDARSIWNTWPVWGINHTFPIGPVK**G GERDGEHLSLPAVSGEEPDAVGPVPAAAAAATLS GVPPLLVRDSEQNVPDTSDGPFRRNGHVCRNRH GALSVHFLH*IQPPREEASAD*GQAAQINMEPH HGSPVQVGPSHRCLLADSLLRPQGHQKPEGAGPK WKLAEPLCSEESL*DPETPSLPPGDPAVGWLWP HS*GLQGPCLWAESQPDPDRAGPELQCAHGCWS QTPLPETETAELQATATAAGQLWPHV*LLPGPGL CA*CQPQPEGARPAAEQPG*RWRATAL*GAQAS CLQTHTPGETKCDDPY*GPGYGRDE**HILTQA AETRIREGGFPCCSG*SQTPGREQDLPNC*DCRG KLPRGSTGGTLVRAFSCLSRGPAYEAFGD*R*L LGPHGACGY*GS*QRKELVPSSLPCSWLLPLAQ HGSLLCDERSGDR*D*ILCVGPVPG*DQPTAQLD GGRASAGHQG*AWSCGSCAPPSLCGSPRGPCGH IPVPNGPL*RGGDAPGEASQGGAASHSSGKPQL LPLGSPPENDP*CPALHSRHLCGVALPPRPS*GS HLPPLPDPK*LLHSEGHR*SRNEIPVCANPQAT PADPTLYGLSLHCVWVWFRDAGNTPQGTGALLS KPWRRPAVLGVLRWPLGIRDQAASERQER*DSGV GGLGETRRSHACNYSDPSSPHSRTFTSGCPAVA ALCGPVSRAADSPSDIGGGCLGQTAWTGAEPGA VREGAG*EHEAQPDAEAVQLEPVLGPEVQRWTLP SPEGDPSSPHYGTLGEGQQKGTPATQQLKYQHQ PLTLESWLWLTLLWVSVSFSANKLPSGLPSSTK VMEL**CLCWALCVHARDATGGPSPGGLTASQGM SIWSWQDPCRPHRASSGGHSSQA*SPPDPIQAQ RGIGGTWNHLPLAVSQGEPQNWGSAWEATWILG FVQEDLQEQANRVKWKEVYSENKGVSQLF*NLSS RLSSFYQKTPIN*KFFT*I*ELKKYKKEKMKIK E*EVTYS

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNSERCPHPISRHHISPHPHPPCLALDPI PGPPYQLTSSSVLQAPLGSSLPWLFLPLPLYRRL SVGALGFIKLGSEC*IRDGKSQGKGQHCSLPA* YPHHLGTSPRHPLNSGTEMAGGAWGRLACYLEF LKKEELKEFQLLLANKAHSRSSSGETPAQPEKTS GMEVASYLVAQYGEQRAWDLALHTWEQMGLRSL CAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTA VLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWR EISASLLYQALPSSPDHESPSQESPNAPTSTAV LGSWGSPPQPSLAPREQEAPGTQWPLDETSGIY YTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQ PHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLL LLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIR DLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKE AWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAE LIGKDGTATPAPIRQILSRPERLLFILDGVDEP GWVLQEPSSELCLHWSQPQPADALLGSLLGKTIL PEASFLITARTTALQNLIPSLEQARWVEVLGFS ESSRKEYFYRYFTDERQAIRAFRLVKSNKELWA LCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTT LCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQK KTLFSPDDLRKHGLDGAIISTFLKMGILQEHPI PLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNC IIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHS LESLHCLYETRNKTFLTQVMAHFEEMGMCVETDM ELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPT MVVLFRWVPVTDAYWQILFSVLKVTRNLKELDL SGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGL TAEDCKDLAFGLRANQTLTELDLSFNVLTDAGA KHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLA SVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHP ACKLIRLGKPSVMTPTEGLDTGEMSNSTSSLKR QRLGSERAASHVAQANLKLLDVSKIFPIAEIAE ESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDF WGPTGPVATEVVDKEKNLYRVHFPVAGSYRWPN TGLCFVMREAVTVEIEFCVWDQFLGEINPQHSW MVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDT SLFQMAHFKEEGMLLEKPARVELHHIVLENPSF SPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEE VTFHLYLIPSDCSIRKAIDDLEMKFQFVRIHKPP PLTPLYMGCRYTVSGSGSGMLEILPKELELCYR SPGEDQLFSEFYVGHLGSGIRLQVKDKKDETLV WEALVKPGDLMPATTLIPPARIAVPSPLDAPQLL HFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQ YERVLAENTRPSQMRKLFSLSQSWDRKCKDGLY QALKETHPHLIMELWEKGSKKGLLPLSS*SINTS P*PLSPGFG*PFFGSQFLSLQTSCHLVCLPALK *WNFDDAFAGHYVSMPGMPQGAPVQVA*QHLRE CPSGAGKTPADLIEPHLVATAAKPRALRIPSRRK EE*EGHGTICLWLCHRVSPKIGVQRGRPRGFLA LYRKIYKSKPTE*SGRKFIQKIKEYHSSFRICL AGFPVFTRKPL*IKNFLLKFKN*KNTKKKK*K*R NKKLPTP

Sequence NALP1.fAug05

TTGTTGACTAGGCGCTGTTCTTGCTGGCTGGTGCCCCAGGGCCTGGAGAGGTCTGAAGAAACCTGGGAGCCAGCAGCCCGGGGCTCCACTCTGGGTTCTG AAAGCCCATTCCCTGCTCTGCGGCTCCTCCCACCCCACCTCTTCTCAGCCTTGCAGCTCAAGGGTTGATCTCAGGAGTCCAGGACCCAGGAGAGGGAAGA ATCTGAGGAACACAGAACAGTGAGCGTTGCCCACACCCCATCTCCCGTCACCACATCTCCCCTCACCCTCACCCTCCCTGCCTGGCCCTGGACCCCATCC CAGGACCTCCCTATCAGCTGACTTCTTCCAGTGTCTTGCAGGCCCCTCTGGGCTCCTCCCTCCCCTGGCTTTTCCTACCACTCCCCCTCTATCGGCGTCT ATCTGTAGGTGCCCTGGGATTTATAAAACTGGGTTCCGAATGCTGAATAAGAGACGGTAAGAGCCAAGGCAAAGGACAGCACTGTTCTCTGCCTGCCTGA TACCCTCACCACCTGGGAACATCCCCCAGACACCCTCTTAACTCCGGGACAGAGATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCC TGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAG TGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTG TGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAG TGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAG AGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTG CTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACT ACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACA GCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTA CTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAG ACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGA AGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAG CTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAG GATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACT TCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCT GAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCC TGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAAC CCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAA AAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCC CTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTG CATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGG GAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTC TGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACAT GGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACC ATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAA GTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCT CACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCC AAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCT CTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCC TGCCTGCAAACTCATACGCCTGGGGCTGGACCAGACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTC ATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCG GATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGGCAAGAGCCACGA GGAAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGAC TTCTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCA ACACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTG GATGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGAC ACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCT TCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGA AGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCA CCCCCGCTGACCCCACTTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTATC GAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGT GTGGGAGGCCTTGGTGAAACCAGGAAGGAACACCAGCCAGCCGTGGAACCTCAGGTGCAACAGAGACGCCAGGAGATACTAGTGCCCAGCAGCCTGCGGC AGTACCAATGAAGCCAGAGAGGGCTTGGTGGATGACAAGGAGGCCTGAGTAGACCGCAGACTTTGTTGTCGCTGCCCGGAGGAGTCGAGACTGGTACCCG GAGGAGCTGTCTCACCAGGAGACCACGTCCTGGAAGTGTCCGGGACTCGCGGGACCTGTGGCTGCAGACCCCGCCGGCACGCAGGCCCAGAGCTGGCGCA CTCCTGAGGATGAGACTCTGGGGGCCCTAGCCGGGGTCCACGGGAGGGCTGTCCTTGGGGACTCTAGGATGGCTTCGTTCTGGCCCGGCTCACTTCTGGA GCTGTGAGACCCAAGACAAAAGGGGCTGAGGGATTTCTCATTGACAAGAGTTCGTGCGGGAAAACCACCTGATCCCTAGGGATTTGTCATCTTAAGACTC AAAAGGCTTAATACCAGGAACCACCTTGGCAAGATATTTACCCACCGGCCATCTCTGTTTACTCATGAATGTTAAATGTTAAAACGCAGCGCTCTAACCC TGCATATTATTTACTTGCAAATGTCTGTAATCTGTAATTGTGATGCCTCTGATGGAATAAATTATCTTTTTCAGTCTCCTCTATA

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

LLTRRCSCWLVPQGLERSEETWEPAARGSTLGS ESPFPALRLLPPHLFSALQLKG*SQESRTQEREE SEEHRTVSVAHTPSPVTTSPLTLTLPAWPWTPS QDLPIS*LLPVSCRPLWAPPSPGFSYHSPSIGV YL*VPWDL*NWVPNAE*ETVRAKAKDSTVLCLPD TLTTWEHPPDTLLTPGQRWLAEPGAAWPVTWSS *RRRS*RSSSFCSPIKRTPGALRVRHPLSQRRR VAWRWPRTWWLSMGSSGPGT*PSIPGSRWG*GHC APKPRKGQATLPHSPTAQVNPTWGLPANPPPPQ C*CPGSMNCRRGAPRAQREGF*DSCLTHLDAAG EKSLPHSSTKLFQAPQTMSLQARSHPTPPHPQQC WGAGDPHLSPA*HPESRRLLGPNGLWMKRQEFT TQKSEKERERNQRKAGPHGQRW*ERPHRRTPAY SPTTTHGSLL*ERASVPHGPGKMRILTKNSHSCY FYKDLTPEAKIPWSREAGLIMWRRIEDI*LRSE TYLAQAWIPKNLA*SYCRGLLELGSQHWPGR*R KPGGEASCMGTASSMSSTSAAESWPSPRW*VSLS SSEKMGQPLRLPLDRSCLGQSGCSSSSMV*MSQ DGSCRSRVLSSVCTGASHSRRMHCWAVCWGKLY FPRHPS*SRLGPQLCRTSFLLWSRHVG*RSWGSL SPAGRNISTDISQMKGKQLEPLGWSNQTKSSGP CVLCPGCPGWPALA*CSR*SGRKNSH*LPRPPQ PSVYITLPRLSKLSHWDPSSETSALWLLRASGKK RPFSVQMTSGSMG*MGPSSPPS*RWVFFKSTPS L*ATASFTSVSKSSLQQCPMSWRMRRGEVNILI AS*IWKRR*KHMEYMACLGHQPHVSYWAC*VMRG RERWRTSFTAGCLRGGT*CSGSRPCSCCCSHTL WSPSTACTRLGTKRS*HK*WPISKKWACV*KQT WSS*CALSALNSAAT*RSFS*LRAGSTDQHGAPP W*SCSGGSQSQMPIGRFSSPSSRSPET*RSWT* VETR*ATLQ*RVFVRP*DALAASWRPCGWLAVA SQLRTARTLPLG*EPTRP*PSWT*ASMCSRMLEP NTFARD*DSRAASYSDCSWSAVASRLTAARTWP LCLVPAPA*RS*TCSRTTWMTLACDCSVRGSGI LPANSYAWGWTRQL*VMR*GRN*GPWSRRNLSCS SSADGNQV**PLLRAWIRER*VIAHPHSSGRDS DQRGRLPMLLRLISNSWT*ARSSQLLRLQARAT RKAPQR*YRWNSCACLLLPLKGTCIRSLWGLTMT SGAPRGLWLLR*LTKKRTCTEFTSL*LAPTAGP TRVSAL**EKR*PLRLNSVCGTSSWVRSTHSTA GWWQGLCWTSRLSLELWKLCTSLTLWLSKGAMWT HPCSKWPTLKRRGCSWRSQPGWSCIT*FWKTPA SPPWESS*K*SIMPCASFPSPLWCCFTTASILR KSPSTST*SQVTAPFGRP*MI*K*NSSLCESTSH PR*PHFIWAVVTLCLGLVQGCWKYSPRNWSSAI EALEKTSCSRSSTLATWDQGSGCK*KTRKMRLW CGRPW*NQEGTPASRGTSGATETPGDTSAQQPAA VPMKPERAWWMTRRPE*TADFVVAARRSRDWYP EELSHQETTSWKCPGLAGPVAADPAGTQAQSWR TPEDETLGALAGVHGRAVLGDSRMASFWPGSLLE L*DPRQKGLRDFSLTRVRAGKPPDP*GFVILRL KRLNTRNHLGKIFTHRPSLFTHEC*MLKRSALT LHIIYLQMSVICNCDASDGINYLFQSPL

Frame 1

C*LGAVLAGWCPRAWRGLKKPGSQQPGAPLWVL KAHSLLCGSSHPTSSQPCSSRVDLRSPGPRRGK NLRNTEQ*ALPTPHLPSPHLPSPSPSLPGPGPHP RTSLSADFFQCLAGPSGLLPPLAFPTTPPLSAS ICRCPGIYKTGFRMLNKRR*EPRQRTALFSACL IPSPPGNIPQTPS*LRDRDGWRSLGPPGLLLGVP EEGGAEGVPASARQ*SALQELFG*DTRSAREDE WHGGGLVPGGSVWGAAGLGPSPPYLGADGAEVT VRPSPGRGRPLSLIPLQPK*TPPGVSQPTHLHRS ANALDP*IAGGVHPGLREKGFETAA*HIWTPLE RNLCLTPLPSSSKLPRP*VSKPGVTQRPHIHSS AGELGIPTSAQPSTQRAGGSWDPMASG*NVRNLL HRNQRKREREIRERQAPMGSGGRNAPTGAHQPT APPPPMGAFCEREPLFHMALEK*GF*PKIHTAA TSTKTSPQKPRSPGQEKLA*LCGGESRTFN*DQR LIWPRPGYPRTSHSHTAGGCWNWEVNTGQAGEG SLGERPAVWGPLPACLLLQLQRAGPVQGGESR* AHRKRWDSHSGSH*TDPV*ARAAALHPRWCR*AR MGLAGAEF*ALSALEPATAGGCTAGQFAGENYT SRGILPDHGSDHSSAEPHSFFGAGTLGRGPGVL *VQQEGIFLQIFHR*KASN*SL*VGQIKQRALGP VSCALGVLAGLHLPDAADEAEGKTHTDFQDHHN PLSTLPCPGSPSSAIGTPAQRPLLSGC*GHLAK KDPFQSR*PQEAWVRWGHHLHLLEDGYSSRAPHP SELQLHSPLFPRVLCSNVLCLGG*EGER*TF*L HHRFGKDARSIWNTWPVWGINHTFPIGPVK**G GERDGEHLSLPAVSGEEPDAVGPVPAAAAAATLS GVPPLLVRDSEQNVPDTSDGPFRRNGHVCRNRH GALSVHFLH*IQPPREEASAD*GQAAQINMEPH HGSPVQVGPSHRCLLADSLLRPQGHQKPEGAGPK WKLAEPLCSEESL*DPETPSLPPGDPAVGWLWP HS*GLQGPCLWAESQPDPDRAGPELQCAHGCWS QTPLPETETAELQATATAAGQLWPHV*LLPGPGL CA*CQPQPEGARPAAEQPG*RWRATAL*GAQAS CLQTHTPGAGPDNSE**DEAGTEGPGAGETSAA HLQQTETKCDDPY*GPGYGRDE**HILTQAAETR IREGGFPCCSG*SQTPGREQDLPNC*DCRQEPR GKLPRGSTGGTLVRAFSCLSRGPAYEAFGD*R* LLGPHGACGY*GS*QRKELVPSSLPCSWLLPLAQ HGSLLCDERSGDR*D*ILCVGPVPG*DQPTAQL DGGRASAGHQG*AWSCGSCAPPSLCGSPRGPCG HIPVPNGPL*RGGDAPGEASQGGAASHSSGKPQL LPLGSPPENDP*CPALHSRHLCGVALPPRPS*G SHLPPLPDPK*LLHSEGHR*SRNEIPVCANPQA TPADPTLYGLSLHCVWVWFRDAGNTPQGTGALLS KPWRRPAVLGVLRWPLGIRDQAASERQER*DSG VGGLGETRKEHQPAVEPQVQQRRQEILVPSSLR QYQ*SQRGLGG*QGGLSRPQTLLSLPGGVETGTR RSCLTRRPRPGSVRDSRDLWLQTPPARRPRAGA LLRMRLWGP*PGSTGGLSLGTLGWLRSGPAHFW SCETQDKRG*GISH*QEFVRENHLIPRDLSS*DS KGLIPGTTLARYLPTGHLCLLMNVKC*NAAL*P CILFTCKCL*SVIVMPLME*IIFFSLLY

Frame 2

VD*ALFLLAGAPGPGEV*RNLGASSPGLHSGF* KPIPCSAAPPTPPLLSLAAQGLISGVQDPGEGR I*GTQNSERCPHPISRHHISPHPHPPCLALDPI PGPPYQLTSSSVLQAPLGSSLPWLFLPLPLYRRL SVGALGFIKLGSEC*IRDGKSQGKGQHCSLPA* YPHHLGTSPRHPLNSGTEMAGGAWGRLACYLEF LKKEELKEFQLLLANKAHSRSSSGETPAQPEKTS GMEVASYLVAQYGEQRAWDLALHTWEQMGLRSL CAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTA VLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWR EISASLLYQALPSSPDHESPSQESPNAPTSTAV LGSWGSPPQPSLAPREQEAPGTQWPLDETSGIY YTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQ PHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLL LLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIR DLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKE AWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAE LIGKDGTATPAPIRQILSRPERLLFILDGVDEP GWVLQEPSSELCLHWSQPQPADALLGSLLGKTIL PEASFLITARTTALQNLIPSLEQARWVEVLGFS ESSRKEYFYRYFTDERQAIRAFRLVKSNKELWA LCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTT LCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQK KTLFSPDDLRKHGLDGAIISTFLKMGILQEHPI PLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNC IIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHS LESLHCLYETRNKTFLTQVMAHFEEMGMCVETDM ELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPT MVVLFRWVPVTDAYWQILFSVLKVTRNLKELDL SGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGL TAEDCKDLAFGLRANQTLTELDLSFNVLTDAGA KHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLA SVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHP ACKLIRLGLDQTTLSDEMRQELRALEQEKPQLL IFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRL GSERAASHVAQANLKLLDVSKIFPIAEIAGKSHE ESSPEVVPVELLCVPSPASQGDLHTKPLGTDDD FWGPTGPVATEVVDKEKNLYRVHFPVAGSYRWP NTGLCFVMREAVTVEIEFCVWDQFLGEINPQHSW MVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVD TSLFQMAHFKEEGMLLEKPARVELHHIVLENPS FSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEE VTFHLYLIPSDCSIRKAIDDLEMKFQFVRIHKP PPLTPLYMGCRYTVSGSGSGMLEILPKELELCY RSPGEDQLFSEFYVGHLGSGIRLQVKDKKDETLV WEALVKPGRNTSQPWNLRCNRDARRY*CPAACG STNEAREGLVDDKEA*VDRRLCCRCPEESRLVP GGAVSPGDHVLEVSGTRGTCGCRPRRHAGPELAH S*G*DSGGPSRGPREGCPWGL*DGFVLARLTSG AVRPKTKGAEGFLIDKSSCGKTT*SLGICHLKT QKA*YQEPPWQDIYPPAISVYS*MLNVKTQRSNP AYYLLANVCNL*L*CL*WNKLSFSVSSI

Sequence NALP1.gAug05

AACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACATGGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGC CGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACCATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATG CCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAAGTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCT TTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCTCACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTG AGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCCAAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCA AGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCTCTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGA CCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCCTGCCTGCAAACTCATACGCCTGGGGCTGGACCAGACA ACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTCATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTA CTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAA TCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGGCAAGAGCCACGAGGAAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTG TGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAG TTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGAC CGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAG CCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGG GGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAA TGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGC TCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCACCCCCGCTGACCCCACTTTATATGGGCTGTCGTTACA CTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTA CGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAAGGAACACCAGC CAGCCGTGGAACCTCAGGTGCAACAGAGACGCCAGGAGATACTAGTGCCCAGCAGCCTGCGGCAGTACCAATGAAGCCAGAGAGGGCTTGGTGGATGACA AGGAGGCCTGAGTAGACCGCAGGTGGGTCTGAGAAATGGGCTTAGGTGAGGCAGGTCTTTGAAGGATTTGTTCTTAATCATATGCGAGATGCTCAAAAGG CTGGATGCCTGCTTTTGTGGGTGAAGAGCAAGAAGAGAAAACAGGTTGTACACATACAGATGCAGATGGAGAGACAGAGAAAAAAAAGGAAGAAGGCAGA GAAATGCACCAATTCTTGAGCTGTATTATCTCTGGACCTTGGGATTGTGGGAGGCTTTATTTTACTACTGATTTTGCCTACACTGTTTTCTCAATTTCTA GTTTTCTACAAAGATGATGTGTTAGCTTTTTCACGCATTAAGATTAAAATTTAAAACAGACCACACAATAAATATTTCTTTTAAAAGGAGAAATATGCCC TGGGGAAGCTCCCCACGCAGCTGAGAGCCTGGCTGGTTGCATTTCGGCTTCTTAGGTGCCAAGCCTACTAGTGCCCCGGGGTCCCAGCCAGCGGCTCGCG TTTCCTCCCCCTTGCGCCGCCAGTCTGGTTGCCATGGAGACAAACCTTGCTGCGCAGAGACTGCCGAGCCGCAGGCAGCGGAGCTGGAACCCGGCGAGCC AGCCTCTGCAACAGGCTGGGGGCGGAAGGAGGAGCCAGGCGAAGCGGCGCCTCAGCTGAGAGGACCGGCGGACCCTGCAGAGGCCCCCTGCCCCTCTGGC TCCGCCCCCACCCGGGTCGCTAGAAATACAGCCGTAGCCCCGCCCACCGCCCACTGCGCTCTGACCCAGACCCGGCTGACCCACCTACCCGCGATCCTGC CCATGGCTGACGGGCTCTTTCGGCGCAGACCCTGGGGTCTCGAGCAGATTCGCCCGGACCCCGAGTCCGAAGGCCTGTTTGACAAGCCTCCCCCGGAAGA CCCTCCCGCTGCCCGCGGGCCCAGGTCGGCGTCGGCCGCGGGCAAGAAGGCTGGTCGGCGCGCGGGCGGGAGGGCGCAGGGGGGCCGCGCCGGGCAGCCC CCGAAGGCCGCATCGCGCCCCCCGCCCAAGAAGGAGGCGCCTCCACTGGACGAGGGCTGCTATCTCGACCATTTTCCGCACCTCTCCATCTTCATCTACG CAGCCATCGCCTTCTCCATCACCTCCTGCATCTTTACCTATATCCATTTACAGCTTGCCTGAGTGGCCAGCGCGGGACGGGGTGGGCGCAGGACCGAGCG GGGAGGGAAAGGGAAAACGGGGCTCGGCATTTTGTGTTTTAGAACAGCGCTGCACCCCCTTCATGTAGCTTTCGATGCTTGTTTCTTTCCGTCTTTGTTG TCACTATCTTTGTCTATCAGTACGAAAGTACAAAGTAGCTGCCGGCAATGAAATAGGGGTGCTGTTTGCACCTGCAGGTTAGGGGTGGAGGCGTTTAGAA TTTTGGGGTGTGATTGAGCCCCGTTTATAATTAGAATGCCCCTGGACCCCTACCACTCTGTGACGTGGGGGCACGCGCAGGGATCCCATCATTTTGTGTT TGGGGAGCTCAGAGTGCGCCCAATCTTGGAATCTTTAAGGGATGAGCCAGACCCAGACCCGCGGCCTTCTAGAGAGGGTCCGGCAGGGAGGGTCGGCGCC CTGGCCCGGGGTGGGCCGGAGCCCTGTGATGCTGCATCGCCCCCAGGAGGAGCCAGCTGTGCCCCAGAGTTGGCGCGGCCGAGAGAGGACAAGAGCGCGC AGCAGGCGAAGCTGGAGGGCGGGACTCGGTAAGTGGCGTTCGTCGGGGTGTCGTGCTGCGCCCCCAGGGGCTCCGGCTGACCACGACTGTGTGTTTTTCC TGCCTTAGACTTTGTTGTCGCTGCCCGGAGGAGTCGAGACTGGTACCCGGAGGAGCTGTCTCACCAGGAGACCACGTCCTGGAAGTGTCCGGGACTCGCG GGACCTGTGGCTGCAGACCCCGCCGGCACGCAGGCCCAGAGCTGGCGCACTCCTGAGGATGAGACTCTGGGGGCCCTAGCCGGGGTCCACGGGAGGGCTG TCCTTGGGGACTCTAGGATGGCTTCGTTCTGGCCCGGCTCACTTCTGGAGCTGTGAGACCCAAGACAAAAGGGGCTGAGGGATTTCTCATTGACAAGAGT TCGTGCGGGAAAACCACCTGATCCCTAGGGATTTGTCATCTTAAGACTCAAAAGGCTTAATACCAGGAACCACCTTGGCAAGATATTTACCCACCGGCCA TCTCTGTTTACTCATGAATGTTAAATGTTAAAACGCAGCGCTCTAACCCTGCATATTATTTACTTGCAAATGTCTGTAATCTGTAATTGTGATGCCTCTG ATGGAATAAATTATCTTTTTCAGTCTCCTCT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

NVPDTSDGPFRRNGHVCRNRHGALSVHFLH*IQ PPREEASAD*GQAAQINMEPHHGSPVQVGPSHRC LLADSLLRPQGHQKPEGAGPKWKLAEPLCSEES L*DPETPSLPPGDPAVGWLWPHS*GLQGPCLWA ESQPDPDRAGPELQCAHGCWSQTPLPETETAELQ ATATAAGQLWPHV*LLPGPGLCA*CQPQPEGAR PAAEQPG*RWRATAL*GAQASCLQTHTPGAGPD NSE**DEAGTEGPGAGETSAAHLQQTETKCDDPY *GPGYGRDE**HILTQAAETRIREGGFPCCSG* SQTPGREQDLPNC*DCRQEPRGKLPRGSTGGTL VRAFSCLSRGPAYEAFGD*R*LLGPHGACGY*GS *QRKELVPSSLPCSWLLPLAQHGSLLCDERSGD R*D*ILCVGPVPG*DQPTAQLDGGRASAGHQG* AWSCGSCAPPSLCGSPRGPCGHIPVPNGPL*RGG DAPGEASQGGAASHSSGKPQLLPLGSPPENDP* CPALHSRHLCGVALPPRPS*GSHLPPLPDPK*L LHSEGHR*SRNEIPVCANPQATPADPTLYGLSLH CVWVWFRDAGNTPQGTGALLSKPWRRPAVLGVL RWPLGIRDQAASERQER*DSGVGGLGETRKEHQ PAVEPQVQQRRQEILVPSSLRQYQ*SQRGLGG*Q GGLSRPQVGLRNGLR*GRSLKDLFLIICEMLKR LDACFCG*RARRENRLYTYRCRWRDREKKGRRQ RNAPILELYYLWTLGLWEALFYY*FCLHCFLNF* FSTKMMC*LFHALRLKFKTDHTINISFKRRNMP WGSSPRS*EPGWLHFGFLGAKPTSAPGSQPAAR VSSPLRRQSGCHGDKPCCAETAEPQAAELEPGEP ASATGWGRKEEPGEAAPQLRGPADPAEAPCPSG SAPTRVARNTAVAPPTAHCALTQTRLTHLPAIL PMADGLFRRRPWGLEQIRPDPESEGLFDKPPPED PPAARGPRSASAAGKKAGRRAGGRAQGGRAGQP PKAASRPPPKKEAPPLDEGCYLDHFPHLSIFIY AAIAFSITSCIFTYIHLQLA*VASAGRGGRRTER GGKGKTGLGILCFRTALHPLHVAFDACFFPSLL SLSLSISTKVQSSCRQ*NRGAVCTCRLGVEAFR ILGCD*APFIIRMPLDPYHSVTWGHAQGSHHFVF GELRVRPILESLRDEPDPDPRPSREGPAGRVGA LARGGPEPCDAASPPGGASCAPELARPREDKSA QQAKLEGGTR*VAFVGVSCCAPRGSG*PRLCVFP ALDFVVAARRSRDWYPEELSHQETTSWKCPGLA GPVAADPAGTQAQSWRTPEDETLGALAGVHGRA VLGDSRMASFWPGSLLEL*DPRQKGLRDFSLTRV RAGKPPDP*GFVILRLKRLNTRNHLGKIFTHRP SLFTHEC*MLKRSALTLHIIYLQMSVICNCDAS DGINYLFQSP

Frame 1

TFLTQVMAHFEEMGMCVETDMELLVCTFCIKFS RHVKKLQLIEGRQHRSTWSPTMVVLFRWVPVTD AYWQILFSVLKVTRNLKELDLSGNSLSHSAVKSL CKTLRRPRCLLETLRLAGCGLTAEDCKDLAFGL RANQTLTELDLSFNVLTDAGAKHLCQRLRQPSC KLQRLQLVSCGLTSDCCQDLASVLSASPSLKELD LQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQT TLSDEMRQELRALEQEKPQLLIFSRRKPSVMTP TEGLDTGEMSNSTSSLKRQRLGSERAASHVAQAN LKLLDVSKIFPIAEIAGKSHEESSPEVVPVELL CVPSPASQGDLHTKPLGTDDDFWGPTGPVATEV VDKEKNLYRVHFPVAGSYRWPNTGLCFVMREAVT VEIEFCVWDQFLGEINPQHSWMVAGPLLDIKAE PGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEE GMLLEKPARVELHHIVLENPSFSPLGVLLKMIHN ALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDC SIRKAIDDLEMKFQFVRIHKPPPLTPLYMGCRY TVSGSGSGMLEILPKELELCYRSPGEDQLFSEFY VGHLGSGIRLQVKDKKDETLVWEALVKPGRNTS QPWNLRCNRDARRY*CPAACGSTNEAREGLVDD KEA*VDRRWV*EMGLGEAGL*RICS*SYARCSKG WMPAFVGEEQEEKTGCTHTDADGETEKKKEEGR EMHQFLSCIISGPWDCGRLYFTTDFAYTVFSIS SFLQR*CVSFFTH*D*NLKQTTQ*IFLLKGEICP GEAPHAAESLAGCISAS*VPSLLVPRGPSQRLA FPPPCAASLVAMETNLAAQRLPSRRQRSWNPAS QPLQQAGGGRRSQAKRRLS*EDRRTLQRPPAPLA PPPPGSLEIQP*PRPPPTAL*PRPG*PTYPRSC PWLTGSFGADPGVSSRFARTPSPKACLTSLPRK TLPLPAGPGRRRPRARRLVGARAGGRRGAAPGSP RRPHRAPRPRRRRLHWTRAAISTIFRTSPSSST QPSPSPSPPASLPISIYSLPEWPARDGVGAGPS GEGKGKRGSAFCVLEQRCTPFM*LSMLVSFRLCC HYLCLSVRKYKVAAGNEIGVLFAPAG*GWRRLE FWGVIEPRL*LECPWTPTTL*RGGTRRDPIILC LGSSECAQSWNL*GMSQTQTRGLLERVRQGGSAP WPGVGRSPVMLHRPQEEPAVPQSWRGRERTRAR SRRSWRAGLGKWRSSGCRAAPPGAPADHDCVFF LP*TLLSLPGGVETGTRRSCLTRRPRPGSVRDSR DLWLQTPPARRPRAGALLRMRLWGP*PGSTGGL SLGTLGWLRSGPAHFWSCETQDKRG*GISH*QE FVRENHLIPRDLSS*DSKGLIPGTTLARYLPTGH LCLLMNVKC*NAAL*PCILFTCKCL*SVIVMPL ME*IIFFSLL

Frame 2

RS*HK*WPISKKWACV*KQTWSS*CALSALNSA AT*RSFS*LRAGSTDQHGAPPW*SCSGGSQSQM PIGRFSSPSSRSPET*RSWT*VETR*ATLQ*RV FVRP*DALAASWRPCGWLAVASQLRTARTLPLG* EPTRP*PSWT*ASMCSRMLEPNTFARD*DSRAA SYSDCSWSAVASRLTAARTWPLCLVPAPA*RS* TCSRTTWMTLACDCSVRGSGILPANSYAWGWTRQ L*VMR*GRN*GPWSRRNLSCSSSADGNQV**PL LRAWIRER*VIAHPHSSGRDSDQRGRLPMLLRL ISNSWT*ARSSQLLRLQARATRKAPQR*YRWNSC ACLLLPLKGTCIRSLWGLTMTSGAPRGLWLLR* LTKKRTCTEFTSL*LAPTAGPTRVSAL**EKR* PLRLNSVCGTSSWVRSTHSTAGWWQGLCWTSRLS LELWKLCTSLTLWLSKGAMWTHPCSKWPTLKRR GCSWRSQPGWSCIT*FWKTPASPPWESS*K*SI MPCASFPSPLWCCFTTASILRKSPSTST*SQVTA PFGRP*MI*K*NSSLCESTSHPR*PHFIWAVVT LCLGLVQGCWKYSPRNWSSAIEALEKTSCSRSS TLATWDQGSGCK*KTRKMRLWCGRPW*NQEGTPA SRGTSGATETPGDTSAQQPAAVPMKPERAWWMT RRPE*TAGGSEKWA*VRQVFEGFVLNHMRDAQK AGCLLLWVKSKKRKQVVHIQMQMERQRKKRKKAE KCTNS*AVLSLDLGIVGGFILLLILPTLFSQFL VFYKDDVLAFSRIKIKI*NRPHNKYFF*KEKYA LGKLPTQLRAWLVAFRLLRCQAY*CPGVPASGSR FLPLAPPVWLPWRQTLLRRDCRAAGSGAGTRRA SLCNRLGAEGGARRSGASAERTGGPCRGPLPLW LRPHPGR*KYSRSPAHRPLRSDPDPADPPTRDPA HG*RALSAQTLGSRADSPGPRVRRPV*QASPGR PSRCPRAQVGVGRGQEGWSARGREGAGGPRRAA PEGRIAPPAQEGGASTGRGLLSRPFSAPLHLHLR SHRLLHHLLHLYLYPFTACLSGQRGTGWAQDRA GRERENGARHFVF*NSAAPPSCSFRCLFLSVFV VTIFVYQYESTK*LPAMK*GCCLHLQVRGGGV*N FGV*LSPVYN*NAPGPLPLCDVGARAGIPSFCV WGAQSAPNLGIFKG*ARPRPAAF*RGSGREGRR PGPGWAGAL*CCIAPRRSQLCPRVGAAERGQERA AGEAGGRDSVSGVRRGVVLRPQGLRLTTTVCFS CLRLCCRCPEESRLVPGGAVSPGDHVLEVSGTR GTCGCRPRRHAGPELAHS*G*DSGGPSRGPREGC PWGL*DGFVLARLTSGAVRPKTKGAEGFLIDKS SCGKTT*SLGICHLKTQKA*YQEPPWQDIYPPA ISVYS*MLNVKTQRSNPAYYLLANVCNL*L*CL* WNKLSFSVSS

Sequence NALP1.lAug05

TTCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGG AAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCC ACCCCCGCTGACCCCACTTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTAT CGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGG TGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGGAAGGAACACCAGCCAGCCGTGGAACCTCAG GTGCAACAGAGACGCCAGGAGATACTAGTGCCCAGCAGCCTGCGGCAGTACCAATGAAGCCAGAGAGGGCTTGGTGGATGACAAGGAGGCCTGAGTAGAC CGCAGGTGGGTCTGAGAAATGGGCTTAGGTGAGGCAGGTCTTTGAAGGATTTGTTCTTAATCATATGCGAGATGCTCAAAAGGCTGGATGCCTGCTTTTG TGGGTGAAGAGCAAGAAGAGAAAACAGGTTGTACACATACAGATGCAGATGGAGAGACAGAGAAAAAAAAGGAAGAAGGCAGAGAAATGCACCAATTCTT GAGCTGTATTATCTCTGGACCTTGGGATTGTGGGAGGCTTTATTTTACTACTGATTTTGCCTACACTGTTTTCTCAATTTCTAGTTTTCTACAAAGATGA TGTGTTAGCTTTTTCACGCATTAAGATTAAAATTTAAAACAGACCACACAATAAATATTTCTTTTAAAAGGAGAAATATGCCCTGGGGAAGCTCCCCACG CAGCTGAGAGCCTGGCTGGTTGCATTTCGGCTTCTTAGGTGCCAAGCCTACTAGTGCCCCGGGGTCCCAGCCAGCGGCTCGCGTTTCCTCCCCCTTGCGC CGCCAGTCTGGTTGCCATGGAGACAAACCTTGCTGCGCAGAGACTGCCGAGCCGCAGGCAGCGGAGCTGGAACCCGGCGAGCCAGCCTCTGCAACAGGCT GGGGGCGGAAGGAGGAGCCAGGCGAAGCGGCGCCTCAGCTGAGAGGACCGGCGGACCCTGCAGAGGCCCCCTGCCCCTCTGGCTCCGCCCCCACCCGGGT CGCTAGAAATACAGCCGTAGCCCCGCCCACCGCCCACTGCGCTCTGACCCAGACCCGGCTGACCCACCTACCCGCGATCCTGCCCATGGCTGACGGGCTC TTTCGGCGCAGACCCTGGGGTCTCGAGCAGATTCGCCCGGACCCCGAGTCCGAAGGCCTGTTTGACAAGCCTCCCCCGGAAGACCCTCCCGCTGCCCGCG GGCCCAGGTCGGCGTCGGCCGCGGGCAAGAAGGCTGGTCGGCGCGCGGGCGGGAGGGCGCAGGGGGGCCGCGCCGGGCAGCCCCCGAAGGCCGCATCGCG CCCCCCGCCCAAGAAGGAGGCGCCTCCACTGGACGAGGGCTGCTATCTCGACCATTTTCCGCACCTCTCCATCTTCATCTACGCAGCCATCGCCTTCTCC ATCACCTCCTGCATCTTTACCTATATCCATTTACAGCTTGCCTGAGTGGCCAGCGCGGGACGGGGTGGGCGCAGGACCGAGCGGGGAGGGAAAGGGAAAA CGGGGCTCGGCATTTTGTGTTTTAGAACAGCGCTGCACCCCCTTCATGTAGCTTTCGATGCTTGTTTCTTTCCGTCTTTGTTGTCACTATCTTTGTCTAT CAGTACGAAAGTACAAAGTAGCTGCCGGCAATGAAATAGGGGTGCTGTTTGCACCTGCAGGTTAGGGGTGGAGGCGTTTAGAATTTTGGGGTGTGATTGA GCCCCGTTTATAATTAGAATGCCCCTGGACCCCTACCACTCTGTGACGTGGGGGCACGCGCAGGGATCCCATCATTTTGTGTTTGGGGAGCTCAGAGTGC GCCCAATCTTGGAATCTTTAAGGGATGAGCCAGACCCAGACCCGCGGCCTTCTAGAGAGGGTCCGGCAGGGAGGGTCGGCGCCCTGGCCCGGGGTGGGCC GGAGCCCTGTGATGCTGCATCGCCCCCAGGAGGAGCCAGCTGTGCCCCAGAGTTGGCGCGGCCGAGAGAGGACAAGAGCGCGCAGCAGGCGAAGCTGGAG GGCGGGACTCGGTAAGTGGCGTTCGTCGGGGTGTCGTGCTGCGCCCCCAGGGGCTCCGGCTGACCACGACTGTGTGTTTTTCCTGCCTTAGACTTTGTTG TCGCTGCCCGGAGGAGTCGAGACTGGTACCCGGAGGAGCTGTCTCACCAGGAGACCACGTCCTGGAAGTGTCCGGGACTCGCGGGACCTGTGGCTGCAGA CCCCGCCGGCACGCAGGCCCAGAGCTGGCGCACTCCTGAGGATGAGACTCTGGGGGCCCTAGCCGGGGTCCACGGGAGGGCTGTCCTTGGGGACTCTAGG ATGGCTTCGTTCTGGCCCGGCTCACTTCTGGAGCTGTGAGACCCAAGACAAAAGGGGCTGAGGGATTTCTCATTGACAAGAGTTCGTGCGGGAAAACCAC CTGATCCCTAGGGATTTGTCATCTTAAGACTCAAAAGGCTTAATACCAGGAACCACCTTGGCAAGATATTTACCCACCGGCCATCTCTGTTTACTCATGA ATGTTAAATGTTAAAACGCAGCGCTCTAACCCTGCATATTATTTACTTGCAAATGTCTGTAATCTGTAATTGTGATGCCTCTGATGGAATAAATTATCTT TTTCAGTCTCCTCT

DEFCAP.au.11.f (forward primer)
NALP1.u.r.25 (reverse primer)
Single exon skipping

Frame 0

FSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPE EVTFHLYLIPSDCSIRKAIDDLEMKFQFVRIHKP PPLTPLYMGCRYTVSGSGSGMLEILPKELELCY RSPGEDQLFSEFYVGHLGSGIRLQVKDKKDETL VWEALVKPGDLMPATTLIPPARIGRNTSQPWNLR CNRDARRY*CPAACGSTNEAREGLVDDKEA*VD RRWV*EMGLGEAGL*RICS*SYARCSKGWMPAF VGEEQEEKTGCTHTDADGETEKKKEEGREMHQFL SCIISGPWDCGRLYFTTDFAYTVFSISSFLQR* CVSFFTH*D*NLKQTTQ*IFLLKGEICPGEAPH AAESLAGCISAS*VPSLLVPRGPSQRLAFPPPCA ASLVAMETNLAAQRLPSRRQRSWNPASQPLQQA GGGRRSQAKRRLS*EDRRTLQRPPAPLAPPPPG SLEIQP*PRPPPTAL*PRPG*PTYPRSCPWLTGS FGADPGVSSRFARTPSPKACLTSLPRKTLPLPA GPGRRRPRARRLVGARAGGRRGAAPGSPRRPHR APRPRRRRLHWTRAAISTIFRTSPSSSTQPSPSP SPPASLPISIYSLPEWPARDGVGAGPSGEGKGK RGSAFCVLEQRCTPFM*LSMLVSFRLCCHYLCL SVRKYKVAAGNEIGVLFAPAG*GWRRLEFWGVIE PRL*LECPWTPTTL*RGGTRRDPIILCLGSSEC AQSWNL*GMSQTQTRGLLERVRQGGSAPWPGVG RSPVMLHRPQEEPAVPQSWRGRERTRARSRRSWR AGLGKWRSSGCRAAPPGAPADHDCVFFLP*TLL SLPGGVETGTRRSCLTRRPRPGSVRDSRDLWLQ TPPARRPRAGALLRMRLWGP*PGSTGGLSLGTLG WLRSGPAHFWSCETQDKRG*GISH*QEFVRENH LIPRDLSS*DSKGLIPGTTLARYLPTGHLCLLM NVKC*NAAL*PCILFTCKCL*SVIVMPLME*IIF FSLL

Frame 1

SPPWESS*K*SIMPCASFPSPLWCCFTTASILR KSPSTST*SQVTAPFGRP*MI*K*NSSLCESTS HPR*PHFIWAVVTLCLGLVQGCWKYSPRNWSSAI EALEKTSCSRSSTLATWDQGSGCK*KTRKMRLW CGRPW*NQEISCLQLL*SLQPA*EGTPASRGTS GATETPGDTSAQQPAAVPMKPERAWWMTRRPE*T AGGSEKWA*VRQVFEGFVLNHMRDAQKAGCLLL WVKSKKRKQVVHIQMQMERQRKKRKKAEKCTNS *AVLSLDLGIVGGFILLLILPTLFSQFLVFYKDD VLAFSRIKIKI*NRPHNKYFF*KEKYALGKLPT QLRAWLVAFRLLRCQAY*CPGVPASGSRFLPLA PPVWLPWRQTLLRRDCRAAGSGAGTRRASLCNRL GAEGGARRSGASAERTGGPCRGPLPLWLRPHPG R*KYSRSPAHRPLRSDPDPADPPTRDPAHG*RA LSAQTLGSRADSPGPRVRRPV*QASPGRPSRCPR AQVGVGRGQEGWSARGREGAGGPRRAAPEGRIA PPAQEGGASTGRGLLSRPFSAPLHLHLRSHRLL HHLLHLYLYPFTACLSGQRGTGWAQDRAGREREN GARHFVF*NSAAPPSCSFRCLFLSVFVVTIFVY QYESTK*LPAMK*GCCLHLQVRGGGV*NFGV*L SPVYN*NAPGPLPLCDVGARAGIPSFCVWGAQSA PNLGIFKG*ARPRPAAF*RGSGREGRRPGPGWA GAL*CCIAPRRSQLCPRVGAAERGQERAAGEAG GRDSVSGVRRGVVLRPQGLRLTTTVCFSCLRLCC RCPEESRLVPGGAVSPGDHVLEVSGTRGTCGCR PRRHAGPELAHS*G*DSGGPSRGPREGCPWGL* DGFVLARLTSGAVRPKTKGAEGFLIDKSSCGKTT *SLGICHLKTQKA*YQEPPWQDIYPPAISVYS* MLNVKTQRSNPAYYLLANVCNL*L*CL*WNKLS FSVSS

Frame 2

LPLGSPPENDP*CPALHSRHLCGVALPPRPS*G SHLPPLPDPK*LLHSEGHR*SRNEIPVCANPQA TPADPTLYGLSLHCVWVWFRDAGNTPQGTGALL SKPWRRPAVLGVLRWPLGIRDQAASERQER*DSG VGGLGETRRSHACNYSDPSSPHRKEHQPAVEPQ VQQRRQEILVPSSLRQYQ*SQRGLGG*QGGLSR PQVGLRNGLR*GRSLKDLFLIICEMLKRLDACFC G*RARRENRLYTYRCRWRDREKKGRRQRNAPIL ELYYLWTLGLWEALFYY*FCLHCFLNF*FSTKM MC*LFHALRLKFKTDHTINISFKRRNMPWGSSPR S*EPGWLHFGFLGAKPTSAPGSQPAARVSSPLR RQSGCHGDKPCCAETAEPQAAELEPGEPASATG WGRKEEPGEAAPQLRGPADPAEAPCPSGSAPTRV ARNTAVAPPTAHCALTQTRLTHLPAILPMADGL FRRRPWGLEQIRPDPESEGLFDKPPPEDPPAAR GPRSASAAGKKAGRRAGGRAQGGRAGQPPKAASR PPPKKEAPPLDEGCYLDHFPHLSIFIYAAIAFS ITSCIFTYIHLQLA*VASAGRGGRRTERGGKGK TGLGILCFRTALHPLHVAFDACFFPSLLSLSLSI STKVQSSCRQ*NRGAVCTCRLGVEAFRILGCD* APFIIRMPLDPYHSVTWGHAQGSHHFVFGELRV RPILESLRDEPDPDPRPSREGPAGRVGALARGGP EPCDAASPPGGASCAPELARPREDKSAQQAKLE GGTR*VAFVGVSCCAPRGSG*PRLCVFPALDFV VAARRSRDWYPEELSHQETTSWKCPGLAGPVAAD PAGTQAQSWRTPEDETLGALAGVHGRAVLGDSR MASFWPGSLLEL*DPRQKGLRDFSLTRVRAGKP PDP*GFVILRLKRLNTRNHLGKIFTHRPSLFTHE C*MLKRSALTLHIIYLQMSVICNCDASDGINYL FQSP