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Sequences

Sequence PTK2B.eAug05

CTGTGAACTCATTGTAATGGCGGGCTCTCTTGATCCTCTCCCTGACCTGGATTCCAGGCCTCTCGGAAAAGGGGGCCAGGAGGGGGCAGGGAGAGGCTGC AATCGCTGCAGATTCTCACTCAGGCAGCAGGACCTCGCTGACCTGCCGTCGGTGGAAGTCAAAAGCATCCCCTTGCCTGAAGCAGGCACTGAAATGTTGG CCTCCAGCTCTGGGAGGTGGGCAGGACAGGCAAGGTGTCAGGAGGGGAAGATCCATATGGCCAGGGTTTCGGTGAGAAGGAGCTGGAGACCCAGGAAACA GTGGCTTGTGTCTAGCTTAGGGAGCTTTTCTTCAAGTTTCTGAAGAATGGGGAGGACAGCTCTGGAACAACAGTCCACTCTCCTTTTATTCCAGCAAGGA TGGAACATTACTGTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGCAGATCA GGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGC AGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAG CGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGTCAGACATCTACGCAGAGATTC CCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGT CTATGAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTGGACAACAAGGAGAAGTTCATG AGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATC CCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTA CCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCTGAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGG TACATTGAGGACGAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCA GTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGA GAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTC ACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGAACCCCCAAAATCTTGG AGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGA GGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAG ATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCG GCTACCGTGAGTGTTCCCGCCCTTCTTCGGGGGGTTTCCTCTTGGCACCTTGTGCAGAGCCACCATCCTTGCCCACCGCCCAGGGCAGGGAGCAGCGGAG TCTGTCTGCATTCCCTGCAGCATCGGCCATGATTTAATTCAGAGCATGTGGGGCAGGCCAGCTTCCCCTCCGCTCCCTCCACCCCATTCCTCTCCCTGCC TAGCACCCTGCCCTTCTCTCTCTCAGTGGCCACATCCTTAGGCCCCTCCTCAGATCATCCCCCTCCTGCATTAGTTTGGAGGAGCTGGAAATTCTCTCTT CCTCATTTCCTGCTCTGTTGGAAGCTCCCGTGGGAAAAGTTAAGCAGTGCCACCTGCCTGTCCCAGTTCAGATTCATCTTCTGTAGGCCTGAGGGACAGA GGAGAGAAAGTGAGAGGGGCAGCTGAGCCCTCAGGCTCTCTCCAGAAGACCCTGTTGCAGGCAGGGAGGGAAGAGCCAGCAGTAAGCCCGGGGCCTGGAG GGGTGGCATTGAGGAGGAGGATGATGACAGCCATCACAGAGAACTGGACACAAGTCAGGCATGGGCCTGGGACCATTTATTATCTTCTGAGGCACAGGGT GATTAGGTAACTTGTCCAAATTCACACAGCTAATAATTGGCAAAGTTCAGATTTGAACCCAAGCCTTCTGACTTTAGGGTCCCTTCTGAGCTCACCCTCT TATCTTACAAATGAAAATTAAGGCTCGGAATGTGAGCTGCCTAGAGTGGTCACAGAGCTCTTTGGCGGCAGAGGTGAGAATAGAAGGCCAGGTGTGGTGG CTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGAGGGTCACTTGATCCCAGGAGTTTGAGACTGGCTTGGGCAACATAGCAAGACCCCATCT CTACAAAAGATTAAGAAAATTAGGCCTGGTGATGCATGCCTGCAAGCCCAGCGGCTCAGGAGGCCGAGGCAGGAGGATTGCCTGAGCCTAGAAGGTGAGG CTACAGTGAGCTGTGATCACGCCACTGTGCTCCAGCCTCGGTGCAAGCCCTGCCTC

PTK2B_x66_x147_f (forward primer)
PTK2B_x66_x147_r (reverse primer)
Single exon skipping

Frame 0

L*THCNGGLS*SSP*PGFQASRKRGPGGGRERL QSLQILTQAAGPR*PAVGGSQKHPLA*SRH*NVG LQLWEVGRTGKVSGGEDPYGQGFGEKELETQET VACV*LRELFFKFLKNGEDSSGTTVHSPFIPAR MEHYCGPGHWP*RDPPAD*SGRKAHLPGRVQADQ VHQVPPAGGGPGSTSAGH*RCPPGLVHQNLIPS RG*EHG*PHRRLLPAAG*APRLSHHPS*ERW*E AEQPAPDPHAKPGGPAVPPLRELQHRVRHLRRDS RRNPAKARRSTVWHCP*RCGPESYSWGRLFWGG L*RCLHKSQRGENQCSCQDLQERLHSGQQGEVH ERGSDHEEPRPPAHREADRHH*RGAHLDHHGIVS LWGAGPLPGAEQELPEGAHPRAVLTADMQSHGL PGEHQLRAQGHCCPEHPGGLP*VCEAGGLWSFP VH*GRGLLQSLCDSSPHQMDVPRVH*LPTLHDSQ *RLDVRRVHVGDPELWEAALLLAGEQGCHRGAG ERRPAAQA*SLSTGPLYPHDPLLGLRPQ*PAPL HRAGVQPQ*RLSDGEGHCHGAREECSLPNPQNLG AHSLPGTPTQAQPT*VQTPSANQPPGSKAAVPG GGLHPTQQPRRGPAAVGG*KGQNAANPGQTAEA DGGGLPVAQAGGEVPGPHGLYE**VPIDAREGGR LP*VFPPFFGGFPLGTLCRATILAHRPGQGAAE SVCIPCSIGHDLIQSMWGRPASPPLPPPHSSPC LAPCPSLSQWPHP*APPQIIPLLH*FGGAGNSLF LISCSVGSSRGKS*AVPPACPSSDSSSVGLRDR GEKVRGAAEPSGSLQKTLLQAGREEPAVSPGPG GVALRRRMMTAITENWTQVRHGPGTIYYLLRHRV IR*LVQIHTANNWQSSDLNPSLLTLGSLLSSPS YLTNEN*GSECELPRVVTELFGGRGENRRPGVV AHTCNPSTLGG*GGRVT*SQEFETGLGNIARPHL YKRLRKLGLVMHACKPSGSGGRGRRIA*A*KVR LQ*AVITPLCSSLGASPA

Frame 1

CELIVMAGSLDPLPDLDSRPLGKGGQEGAGRGC NRCRFSLRQQDLADLPSVEVKSIPLPEAGTEML ASSSGRWAGQARCQEGKIHMARVSVRRSWRPRKQ WLVSSLGSFSSSF*RMGRTALEQQSTLLLFQQG WNITVDLVIGPKGIRQLTSQDAKPTCLAEFKQI RSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLA EAENMADLIDGYCRLQGEHQGSLIIHPRKDGEK RNSLPQIPMLNLEARRSHLSESCSIESDIYAEI PDETLRRPGGPQYGIAREDVVLNRILGEGFFGEV YEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFM SEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELY PYGELGHYLERNKNSLKVLTLVLYSLQICKAMAY LESINCVHRDIAVRNILVASPECVKLGDFGLSR YIEDEDYYKASVTRLPIKWMSPESINFRRFTTA SDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLE KGDRLPKPDLCPPVLYTLMTRCWDYDPSDRPRF TELVCSLSDVYQMEKDIAMEQERNARYRTPKIL EPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQE EDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQ MVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEV GYRECSRPSSGGFLLAPCAEPPSLPTAQGREQRS LSAFPAASAMI*FRACGAGQLPLRSLHPIPLPA *HPALLSLSGHILRPLLRSSPSCISLEELEILS SSFPALLEAPVGKVKQCHLPVPVQIHLL*A*GTE ERK*EGQLSPQALSRRPCCRQGGKSQQ*ARGLE GWH*GGG**QPSQRTGHKSGMGLGPFIIF*GTG *LGNLSKFTQLIIGKVQI*TQAF*L*GPF*AHPL ILQMKIKARNVSCLEWSQSSLAAEVRIEGQVWW LTPVIPALWEAEVGGSLDPRSLRLAWAT*QDPI STKD*EN*AW*CMPASPAAQEAEAGGLPEPRR*G YSEL*SRHCAPASVQALP

Frame 2

VNSL*WRALLILSLTWIPGLSEKGARRGQGEAA IAADSHSGSRTSLTCRRWKSKASPCLKQALKCW PPALGGGQDRQGVRRGRSIWPGFR*EGAGDPGN SGLCLA*GAFLQVSEEWGGQLWNNSPLSFYSSKD GTLLWTWSLALKGSAS*LVRTQSPPAWPSSSRS GPSGASRWRRARQYFSWALKVPPRPCPSKPHP* QRLRTWLTS*TATAGCRVSTKALSSSILGKMVRS GTACPRSPC*TWRPGGPTSQRAAA*SQTSTQRF PTKPCEGPEVHSMALPVKMWS*IVFLGKAFLGR SMKVSTQITKGRKSM*LSRPARKTALWTTRRSS* ARQ*S*RTSTTRTS*S*SASLKRSPPGSSWNCI PMGSWATTWSGTRTP*RCSPSCCTHCRYAKPWP TWRASTACTGTLLSGTSWWPPLSV*SWGTLVFPG TLRTRTITKPL*LVSPSNGCPQSPLTSDASRQP VTSGCSPCACGRS*ALGSSPSSGWRTRMSSGCW RKETGCPSLISVHRSFIPS*PAAGTTTPVTGPAS PSWCAASVTFIRWRRTLPWSKRGMLATEPPKSW SPQPSRNPHPSPADLSTDPLRKPTSWLQSCSSR RRTSSNPAAEKRPSSCGRLKRSKCGKSWTNSRSR WWRTTSGSGRRRSPWTPWFI*MISPH*RQRRRS ATVSVPALLRGVSSWHLVQSHHPCPPPRAGSSG VCLHSLQHRP*FNSEHVGQASFPSAPSTPFLSLP STLPFSLSVATSLGPSSDHPPPALVWRSWKFSL PHFLLCWKLPWEKLSSATCLSQFRFIFCRPEGQ RRESERGS*ALRLSPEDPVAGREGRASSKPGAWR GGIEEEDDDSHHRELDTSQAWAWDHLLSSEAQG D*VTCPNSHS**LAKFRFEPKPSDFRVPSELTL LSYK*KLRLGM*AA*SGHRALWRQR*E*KARCGG SHL*SQHFGRLRWEGHLIPGV*DWLGQHSKTPS LQKIKKIRPGDACLQAQRLRRPRQEDCLSLEGE ATVSCDHATVLQPRCKPCL

Sequence PTK2B.dAug05

GACAGCGGCGCTGGCCAATCGTGCGGGGGGGATGGCGAGGGGGAGGGAGGGGGCGCTCGGAGGAGCCCCACCCACTTCCGGTGTGCGCGGGAAATCTTGG GAGAGCGGGGTGGGCCTTCTGCCCGCAGTTCCCGCCTCCTCAGGTCCGGGCGGGTCCCTGGCCGGGGTAGCACGGAAGGGTCTCCCAGGCGGCGTAGTAG GGCTTCCGTGTTACTGGAAACCTACTTCCGGCTGCAAATGGGAAAAGGAGCCTCTACCTTAACCAATCCCCGGGAACCTCAGGCCCGCGGATGGGAGAAA CCAGAGATGCCAACTTCCTGCTTCCGAAGTAGTGGCTGGGTCTTAAAGCACCGAATAGGACTAGCAACTTCATAGACGGTTGTGAAGACAAGCTAGACGG CAGATGAAAGTTCTTGGCACAGAGTGAACACTTGATAAACTATATGGCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACTTACCTG TACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTG AGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGA TGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACG GAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGT CCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCA AATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGCAGCGC TACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGT CCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCA GCAGACCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATCGACCAGGAGACCTAC CGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCC TGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTC CATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCAT CCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGT CAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGG GGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTG GACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCT GGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCA GATATGCAAAGCCATGGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCTGAGTGTGTGAAGCTG GGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACT TCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAA GGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGCTGCTGGGACTACGAC CCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCT ACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCTGGC TCCAAAGCTGCAGTTCCAGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATC CTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCAT TGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGA CCGGACTGATGACCTGGTGTACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTG GTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAGG GCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAG GCAGATGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTGGCCAATCTGGCCCACCCACCT GCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGCGTCTTCCGCCCCTGCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGG GAAGGCCAAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCACCCCTACCCCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGG ACTCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAGGGGGACTGCTGCTGCCTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGG GGGCTCCTGGGGGTGGGGAGGTGTCACATGGTGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTTTCCCTTCCTCTT CGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTGGGGGTCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGT ACAGTGTATATTGAAATTTATTTAATGTGAGTTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTAAT TGGGAGTCCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCAGGGGGAAGAAGCAGAGAGATGCGGCCAAGATAGGACCTTGGGCCAAATCCGCT CTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTTTCCCTCTTTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGC ACCCTTCTTTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGGTTGAATTAAAATTGTCCCATTTGCTTTGC

PTK2B_x66_x147_f (forward primer)
PTK2B_x66_x147_r (reverse primer)
Single exon skipping

Frame 0

DSGAGQSCGGDGEGEGGGARRSPTHFRCAREIL GERGGPSARSSRLLRSGRVPGRGSTEGSPRRRSR ASVLLETYFRLQMGKGASTLTNPREPQARGWEK PEMPTSCFRSSGWVLKHRIGLATS*TVVKTS*T ADESSWHRVNT**TIWQPQPPEPLHTYLPGRLTC TCRRPGSPGGARGVVAGVRASLGLQCADLSCCL RGCLGCPSP*VE*SWARYAGLKALQSPWWWYQ* MWKRRTCVSSRSASIATASILGKTSNWSNALSRR RSGRSSPPSC*AGGSGPTSGWLSAMG*G*ST*S PMRSTGCTHR*RWVRCRTSMSVCTWKPSGGMTF KSATCQKTSWRA*RRTGPRCSIFTNSSGTTTCSA TPARSARAWPCSWAAWSSGGSSRICPTMHLTRS PTSSS*KRKWGWTCFSQSRCRRT*SPNSSGR*S SRPSSSTPRSGRRSAS*SSSTLSPASPTSTRRPT AVNSFKDGTLLWTWSLALKGSAS*LVRTQSPPA WPSSSRSGPSGASRWRRARQYFSWALKVPPRPC PSKPHP*QRLRTWLTS*TATAGCRVSTKALSSSI LGKMVRSGTACPRSPC*TWRPGGPTSQRAAA*S QTSTQRFPTKPCEGPEVHSMALPVKMWS*IVFL GKAFLGRSMKVSTQITKGRKSM*LSRPARKTALW TTRRSS*ARQ*S*RTSTTRTS*S*SASLKRSPP GSSWNCIPMGSWATTWSGTRTP*RCSPSCCTHC RYAKPWPTWRASTACTGTLLSGTSWWPPLSV*SW GTLVFPGTLRTRTITKPL*LVSPSNGCPQSPLT SDASRQPVTSGCSPCACGRS*ALGSSPSSGWRT RMSSGCWRKETGCPSLISVHRSFIPS*PAAGTTT PVTGPASPSWCAASVTFIRWRRTLPWSKRGMLA TEPPKSWSPQPSRNPHPSPADLSTDPLRKPTSW LQSCSSRRRTSSNPAAEKRPSSCGRLKRSKCGKS WTNSRSRWWRTTSGSGRRRSPWTPWFI*MISPH *RQRRRSATWSSQGPHRSPRGWAHSPSSPQLTW TGLMTWCTSMSWSWCGPCWSSRMSSVSCPPRATW WW*RMWG*PCGSSSGAWMISCLPCRHLHGQRSR APRNCSTKTWQSSSTRCGWHSRTP*PP*VRSAR GRC*RLHTPWLWTPRTCSTLWTRPRFWPIWPTHL QSDGGWGPPACVFRPCLPCTSPALLLVMWVFQG EGQGESPSLATLHDALSPPLPLAVLLRLQLDRG DSGLWTQGDGDKDGSEGDCCCLATAP*ASLVHAG GSWGWGGVTWCP*LYIWTWQADLGTKLFLSLPL RPSDVP*CTEKLGRSFVLGVRQPVR*GMGLAFL YSVY*NLFNVSLVWTDSMCPPEGGPGAQSRNKLI GSPGTGCCVVNKPSIRGKKQRDAAKIGPWAKSA LFLPLFLFLPLLSLAFPSFLTPPLSLPNPHSHL HPSFLMCLHKHSFNFFLFDLWLN*NCPICF

Frame 1

TAALANRAGGMARGREGALGGAPPTSGVRGKSW ESGVGLLPAVPASSGPGGSLAGVARKGLPGGVV GLPCYWKPTSGCKWEKEPLP*PIPGNLRPADGRN QRCQLPASEVVAGS*STE*D*QLHRRL*RQARR QMKVLGTE*TLDKLYGSHSLRSRCTPTCPADLP VLAAVPAHLAVPEE*SLESAPPWDCNVPILAAA* EDVWGVRAPESSKVGHVTPA*RPCRAHGGGTSR CGKGGRAYPQGLLL*QQLQSWEKLQTGQMHCPD GDPGDHHLHPAERADRAQHPVG*VLWAEAEAHEV R*DPLAAPTDDGG*GAGQV*VSARGSRVEV*PS NPLLARRLHGEPEGGQDHAALFLPTAPERLHAA LRQQGQRGHGPAAGLPGAQAVLQGYAPQCT*QEV QLRAPRKGSGAGLVFPKADAGELKAQTVPEDDP ADLPAVRLAQGGGVRHEVLQHSRRLRQHRPGDL PL*THSRMEHYCGPGHWP*RDPPAD*SGRKAHLP GRVQADQVHQVPPAGGGPGSTSAGH*RCPPGLV HQNLIPSRG*EHG*PHRRLLPAAG*APRLSHHP S*ERW*EAEQPAPDPHAKPGGPAVPPLRELQHRV RHLRRDSRRNPAKARRSTVWHCP*RCGPESYSW GRLFWGGL*RCLHKSQRGENQCSCQDLQERLHS GQQGEVHERGSDHEEPRPPAHREADRHH*RGAHL DHHGIVSLWGAGPLPGAEQELPEGAHPRAVLTA DMQSHGLPGEHQLRAQGHCCPEHPGGLP*VCEA GGLWSFPVH*GRGLLQSLCDSSPHQMDVPRVH*L PTLHDSQ*RLDVRRVHVGDPELWEAALLLAGEQ GCHRGAGERRPAAQA*SLSTGPLYPHDPLLGLR PQ*PAPLHRAGVQPQ*RLSDGEGHCHGAREECSL PNPQNLGAHSLPGTPTQAQPT*VQTPSANQPPG SKAAVPGGGLHPTQQPRRGPAAVGG*KGQNAAN PGQTAEADGGGLPVAQAGGEVPGPHGLYE**VPI DAREGGRLPGVHRAPTEAPEAGRTVHPAHS*PG PD**PGVPQCHGAGAGRAGAQE*ALSAAPRGLR GGGEECGADPAEAHRERG*SPAFLAVIFTDRDRG HPETAQQRPGRAHQQDAAGTAERRDLPK*GVQE ADADGFTHPGCGRQEPARRCGPGQGSGQSGPPT CRVTEGGGHLPASSAPACHVPPLPCCWSCGSSRG KAKGSHLPLPLCTTPSPHPYPWLYCSGCSWTEG TLGYGHRVTVTKMAQRGTAAAWPLLPKPAWSMQ GAPGGGEVSHGAPSFIYGHGRPIWEPSYSFPFLF GPQMSLDAQRSWGGALFWGSGSQ*DEGWAWHSC TVYIEIYLM*VWSGLTACALLREDLGHSPGTS* LGVQAQDAVLSTNQASGGRSREMRPR*DLGPNPL SSCPSFSFFLYFPLLFPLFLLLLFLSPTPILIC TLLFSCVCINILLTSFYLTCG*IKIVPFAL

Frame 2

QRRWPIVRGGWRGGGRGRSEEPHPLPVCAGNLG RAGWAFCPQFPPPQVRAGPWPG*HGRVSQAA** GFRVTGNLLPAANGKRSLYLNQSPGTSGPRMGE TRDANFLLPK*WLGLKAPNRTSNFIDGCEDKLDG R*KFLAQSEHLINYMAATASGAVAHLPARPTYL YLPPSRLTWRCPRSSRWSPRLPGTAMCRS*LLP ERMSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVD VEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQT EIREIITSILLSGRIGPNIRLAECYGLRLKHMK SDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQ IRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQR YASKVSEGMALQLGCLELRRFFKDMPHNALDKK SNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQ QTFQQYASLREEECVMKFFNTLAGFANIDQETY RCELIQGWNITVDLVIGPKGIRQLTSQDAKPTC LAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQALS IKTSSLAEAENMADLIDGYCRLQGEHQGSLIIH PRKDGEKRNSLPQIPMLNLEARRSHLSESCSIE SDIYAEIPDETLRRPGGPQYGIAREDVVLNRILG EGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTL DNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPT WIIMELYPYGELGHYLERNKNSLKVLTLVLYSLQ ICKAMAYLESINCVHRDIAVRNILVASPECVKL GDFGLSRYIEDEDYYKASVTRLPIKWMSPESIN FRRFTTASDVWMFAVCMWEILSFGKQPFFWLENK DVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYD PSDRPRFTELVCSLSDVYQMEKDIAMEQERNAR YRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLA PKLQFQEEDFIQPSSREEAQQLWEAEKVKMRQI LDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSP LTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLD RTDDLVYLNVMELVRAVLELKNELCQLPPEGYV VVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIE GTQKLLNKDLAELINKMRLAQQNAVTSLSEECKR QMLTASHTLAVDAKNLLDAVDQAKVLANLAHPP AE*RRVGATCLRLPPLPAMYLPCLAVGHVGLPG GRPRGVTFPCHFARRPLPTPTPGCTAQAAAGQRG LWAMDTG*R*QRWLRGGLLLPGHCSLSQPGPCR GLLGVGRCHMVPLALYMDMAGRFGNQAIPFPSS SALRCPLMHREAGEELCFGGQAASEMRDGPGILV QCILKFI*CEFGLD*QHVPS*GRTWGTVQEQAN WESRHRMLCCQQTKHQGEEAERCGQDRTLGQIR SLPAPLSLSSFTFPCFSLFSYSSSFSPQPPFSSA PFFSHVFA*TFF*LLSI*LVVELKLSHLLC

Sequence PTK2B.cAug05

AGGGGTGTGGTTAACAACTCAGAGGAGGAGGGAGAATCTAACCTGTCAGCCCTTTTACTCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGG CCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTT AGCTGCTGCCTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAGAGCCCATGGTGGT GGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGC ACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGA AGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGTGGAAGCCGAGTGGAG GTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGAC TACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCAC TTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCG GAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATCGAC CAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAA AGCCCACCTGCCTGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGCCCC CCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGCTCT CTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCT GCAGCATAGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGAAGATGTGGTCCTGAA TCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAA GACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAG AGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCT GTACTCACTGCAGATATGCAAAGCCATGGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCTGAG TGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAG AGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTG GCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGCTGC TGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGA GGAATGCTCGCTACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGACCCCCTCCGCAAAC CAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTTAAC TCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGC AGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTC CCTGGACCCCATGGTTTATATGAATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCGAGG CTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCA AGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCT GCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCACAG CAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGG ACCAGGCCAAGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGCGTCTTCCGCCCCTGCCTGCCATGTACCT CCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCCAAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCACCCCTACC CCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAGGGGGACTGCTGCTGC CTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGGAGGTGTCACATGGTGCCCCTAGCTTTATATATGGACATGGCAGG CCGATTTGGGAACCAAGCTATTCCTTTCCCTTCCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTGGGGGTCAG GCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGAAATTTATTTAATGTGAGTTTGGTCTGGACTGACAGCATGTGCCCTCCT GAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTAATTGGGAGTCCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCAGGGGGAAGAAGCAGA GAGATGCGGCCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTTTCCCTCTTTTCTTAC TCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCTTTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGG TTGAATTAAAATTGTCCCATTTGCTTTGC

PTK2B_x66_x147_f (forward primer)
PTK2B_x66_x147_r (reverse primer)
Single exon skipping

Frame 0

RGVVNNSEEEGESNLSALLLSHSLRSRCTPTCP ADLPVLAAVPAHLAVPEE*SLESAPPWDCNVPIL AAA*EDVWGVRAPESSKVGHVTPA*RPCRAHGG GTSRCGKGGRAYPQGLLL*QQLQSWEKLQTGQM HCPDGDPGDHHLHPAERADRAQHPVG*VLWAEAE AHEVR*DPLAAPTDDGG*GAGQV*VSARGSRVE V*PSNPLLARRLHGEPEGGQDHAALFLPTAPER LHAALRQQGQRGHGPAAGLPGAQAVLQGYAPQCT *QEVQLRAPRKGSGAGLVFPKADAGELKAQTVP EDDPADLPAVRLAQGGGVRHEVLQHSRRLRQHR PGDLPL*THSRMEHYCGPGHWP*RDPPAD*SGRK AHLPGRVQADQVHQVPPAGGGPGSTSAGH*RCP PGLVHQNLIPSRG*EHG*PHRRLLPAAG*APRL SHHPS*ERW*EAEQPAPDPHAKPGGPAVPPLREL QHRVRHLRRDSRRNPAKARRSTVWHCP*RCGPE SYSWGRLFWGGL*RCLHKSQRGENQCSCQDLQE RLHSGQQGEVHERGSDHEEPRPPAHREADRHH*R GAHLDHHGIVSLWGAGPLPGAEQELPEGAHPRA VLTADMQSHGLPGEHQLRAQGHCCPEHPGGLP* VCEAGGLWSFPVH*GRGLLQSLCDSSPHQMDVPR VH*LPTLHDSQ*RLDVRRVHVGDPELWEAALLL AGEQGCHRGAGERRPAAQA*SLSTGPLYPHDPL LGLRPQ*PAPLHRAGVQPQ*RLSDGEGHCHGARE ECSLPNPQNLGAHSLPGTPTQAQPT*VQTPSAN QPPGSKAAVPGS*GSVCQLSYAHQPYGVSISR* LTAHPTSPPAQCLQTPQHAGGGLHPTQQPRRGPA AVGG*KGQNAANPGQTAEADGGGLPVAQAGGEV PGPHGLYE**VPIDAREGGRLPGVHRAPTEAPE AGRTVHPAHS*PGPD**PGVPQCHGAGAGRAGAQ E*ALSAAPRGLRGGGEECGADPAEAHRERG*SP AFLAVIFTDRDRGHPETAQQRPGRAHQQDAAGT AERRDLPK*GVQEADADGFTHPGCGRQEPARRCG PGQGSGQSGPPTCRVTEGGGHLPASSAPACHVP PLPCCWSCGSSRGKAKGSHLPLPLCTTPSPHPY PWLYCSGCSWTEGTLGYGHRVTVTKMAQRGTAAA WPLLPKPAWSMQGAPGGGEVSHGAPSFIYGHGR PIWEPSYSFPFLFGPQMSLDAQRSWGGALFWGS GSQ*DEGWAWHSCTVYIEIYLM*VWSGLTACALL REDLGHSPGTS*LGVQAQDAVLSTNQASGGRSR EMRPR*DLGPNPLSSCPSFSFFLYFPLLFPLFL LLLFLSPTPILICTLLFSCVCINILLTSFYLTCG *IKIVPFAL

Frame 1

GVWLTTQRRRENLTCQPFYSATASGAVAHLPAR PTYLYLPPSRLTWRCPRSSRWSPRLPGTAMCRS *LLPERMSGVSEPLSRVKLGTLRRPEGPAEPMVV VPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKC TVQTEIREIITSILLSGRIGPNIRLAECYGLRL KHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWR YDLQIRYLPEDFMESLKEDRTTLLYFYQQLRND YMQRYASKVSEGMALQLGCLELRRFFKDMPHNA LDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFR KMIQQTFQQYASLREEECVMKFFNTLAGFANID QETYRCELIQGWNITVDLVIGPKGIRQLTSQDA KPTCLAEFKQIRSIRCLPLEEGQAVLQLGIEGAP QALSIKTSSLAEAENMADLIDGYCRLQGEHQGS LIIHPRKDGEKRNSLPQIPMLNLEARRSHLSES CSIESDIYAEIPDETLRRPGGPQYGIAREDVVLN RILGEGFFGEVYEGVYTNHKGEKINVAVKTCKK DCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIE EEPTWIIMELYPYGELGHYLERNKNSLKVLTLVL YSLQICKAMAYLESINCVHRDIAVRNILVASPE CVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSP ESINFRRFTTASDVWMFAVCMWEILSFGKQPFFW LENKDVIGVLEKGDRLPKPDLCPPVLYTLMTRC WDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQE RNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQT NLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVN SLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQ QLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKS LDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPR LGAQSIQPTANLDRTDDLVYLNVMELVRAVLEL KNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLL PSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQ QNAVTSLSEECKRQMLTASHTLAVDAKNLLDAV DQAKVLANLAHPPAE*RRVGATCLRLPPLPAMYL PCLAVGHVGLPGGRPRGVTFPCHFARRPLPTPT PGCTAQAAAGQRGLWAMDTG*R*QRWLRGGLLL PGHCSLSQPGPCRGLLGVGRCHMVPLALYMDMAG RFGNQAIPFPSSSALRCPLMHREAGEELCFGGQ AASEMRDGPGILVQCILKFI*CEFGLD*QHVPS *GRTWGTVQEQANWESRHRMLCCQQTKHQGEEAE RCGQDRTLGQIRSLPAPLSLSSFTFPCFSLFSY SSSFSPQPPFSSAPFFSHVFA*TFF*LLSI*LV VELKLSHLLC

Frame 2

GCG*QLRGGGRI*PVSPFTQPQPPEPLHTYLPG RLTCTCRRPGSPGGARGVVAGVRASLGLQCADL SCCLRGCLGCPSP*VE*SWARYAGLKALQSPWW WYQ*MWKRRTCVSSRSASIATASILGKTSNWSNA LSRRRSGRSSPPSC*AGGSGPTSGWLSAMG*G* ST*SPMRSTGCTHR*RWVRCRTSMSVCTWKPSG GMTFKSATCQKTSWRA*RRTGPRCSIFTNSSGTT TCSATPARSARAWPCSWAAWSSGGSSRICPTMH LTRSPTSSS*KRKWGWTCFSQSRCRRT*SPNSS GR*SSRPSSSTPRSGRRSAS*SSSTLSPASPTST RRPTAVNSFKDGTLLWTWSLALKGSAS*LVRTQ SPPAWPSSSRSGPSGASRWRRARQYFSWALKVP PRPCPSKPHP*QRLRTWLTS*TATAGCRVSTKAL SSSILGKMVRSGTACPRSPC*TWRPGGPTSQRA AA*SQTSTQRFPTKPCEGPEVHSMALPVKMWS* IVFLGKAFLGRSMKVSTQITKGRKSM*LSRPARK TALWTTRRSS*ARQ*S*RTSTTRTS*S*SASLK RSPPGSSWNCIPMGSWATTWSGTRTP*RCSPSC CTHCRYAKPWPTWRASTACTGTLLSGTSWWPPLS V*SWGTLVFPGTLRTRTITKPL*LVSPSNGCPQ SPLTSDASRQPVTSGCSPCACGRS*ALGSSPSS GWRTRMSSGCWRKETGCPSLISVHRSFIPS*PAA GTTTPVTGPASPSWCAASVTFIRWRRTLPWSKR GMLATEPPKSWSPQPSRNPHPSPADLSTDPLRK PTSWLQSCSSRFLRVCVPALLRSPALWSIHLPLT HCTPHLSTGTMSSNATACGRRTSSNPAAEKRPS SCGRLKRSKCGKSWTNSRSRWWRTTSGSGRRRS PWTPWFI*MISPH*RQRRRSATWSSQGPHRSPRG WAHSPSSPQLTWTGLMTWCTSMSWSWCGPCWSS RMSSVSCPPRATWWW*RMWG*PCGSSSGAWMIS CLPCRHLHGQRSRAPRNCSTKTWQSSSTRCGWHS RTP*PP*VRSARGRC*RLHTPWLWTPRTCSTLW TRPRFWPIWPTHLQSDGGWGPPACVFRPCLPCT SPALLLVMWVFQGEGQGESPSLATLHDALSPPLP LAVLLRLQLDRGDSGLWTQGDGDKDGSEGDCCC LATAP*ASLVHAGGSWGWGGVTWCP*LYIWTWQ ADLGTKLFLSLPLRPSDVP*CTEKLGRSFVLGVR QPVR*GMGLAFLYSVY*NLFNVSLVWTDSMCPP EGGPGAQSRNKLIGSPGTGCCVVNKPSIRGKKQ RDAAKIGPWAKSALFLPLFLFLPLLSLAFPSFLT PPLSLPNPHSHLHPSFLMCLHKHSFNFFLFDLW LN*NCPICF

Sequence PTK2B.bAug05

GACAGCGGCGCTGGCCAATCGTGCGGGGGGGATGGCGAGGGGGAGGGAGGGGGCGCTCGGAGGAGCCCCACCCACTTCCGGTGTGCGCGGGAAATCTTGG GAGAGCGGGGTGGGCCTTCTGCCCGCAGTTCCCGCCTCCTCAGGTCCGGGCGGGTCCCTGGCCGGGGTAGCACGGAAGGGTCTCCCAGGCGGCGTAGTAG GGCTTCCGTGTTACTGGAAACCTACTTCCGGCTGCAAATGGGAAAAGGAGCCTCTACCTTAACCAATCCCCGGGAACCTCAGGCCCGCGGATGGGAGAAA CCAGAGATGCCAACTTCCTGCTTCCGAAGTAGTGGCTGGGTCTTAAAGCACCGATGTTTCTGCATTGAATAGCCCTGGAAGACAGAATTCTTGTCTCTCC AAAGATCTGGCAGACTTATTCCCAATTATAAGAGATCCTTGCAAAGGAGGTGTCTTGCTTCACCTTCACCTTCCACTGTGATTGTAAGTTTTCTGAGGCC TCGCCAGCCATGCGGAACTGAATAGGACTAGCAACTTCATAGACGGTTGTGAAGACAAGCTAGACGGCAGATGAAAGTTCTTGGCACAGAGTGAACACTT GATAAACTATATGGCAGGGAGGGCTGGAACGGGGCTTGTTTGAAGAGCAATATGAGCCAGGGTTATAGACCTGAGTTTGGGGTAAGAGTGGAACATCCAA ATGGAAGAGTCCAGCAGCTGAAAGGACATTTGTTCAAAGGCCTTTTTCATTACAGTTTTCTCCTTCTTCTGATCCAGCCACAGCCTCCGGAGCCGTTGCA CACCTACCTGCCCGGCCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTG CAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGC AGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTC AAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCT ATGGGCTGAGGCTGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGT GGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAA CAGCTCCGGAACGACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATA TGCCCCACAATGCACTTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAA GCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGC TTCGCCAACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGA CTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGG CATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGT GAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCC ACCTCTCAGAGAGCTGCAGCATAGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGA AGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTC AAGACCTGCAAGAAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGA TCGGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGT GCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTG GTGGCCTCCCCTGAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCA AATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAA GCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACC CTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTG CCATGGAGCAAGAGAGGAATGCTCGCTACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAG ACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTAT CCATCTCCCGTTAACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGGGAGGAGGACTTCATCCAACCCAGCAGCC GAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAGTGGCTCAG GCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCA CAGAAGCCCCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTCAATGTCATGGAGCTGGTGCGGG CCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAG CGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAG ATGCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACC TGCTCGACGCTGTGGACCAGGCCAAGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGCGTCTTCCGCCCCT GCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCCAAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCT CTCCCCACCCCTACCCCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAG GGGGACTGCTGCTGCCTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGGAGGTGTCACATGGTGCCCCTAGCTTTATA TATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTTTCCCTTCCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTT TGTTTTGGGGGTCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGAAATTTATTTAATGTGAGTTTGGTCTGGACTGAC AGCATGTGCCCTCCTGAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTAATTGGGAGTCCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCA GGGGGAAGAAGCAGAGAGATGCGGCCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTT TCCCTCTTTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCTTTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTT CTATTTGACTTGTGGTTGAATTAAAATTGTCCCATTTGCTTTGC

PTK2B_x66_x147_f (forward primer)
PTK2B_x66_x147_r (reverse primer)
Single exon skipping

Frame 0

DSGAGQSCGGDGEGEGGGARRSPTHFRCAREIL GERGGPSARSSRLLRSGRVPGRGSTEGSPRRRSR ASVLLETYFRLQMGKGASTLTNPREPQARGWEK PEMPTSCFRSSGWVLKHRCFCIE*PWKTEFLSL QRSGRLIPNYKRSLQRRCLASPSPSTVIVSFLRP RQPCGTE*D*QLHRRL*RQARRQMKVLGTE*TL DKLYGREGWNGACLKSNMSQGYRPEFGVRVEHP NGRVQQLKGHLFKGLFHYSFLLLLIQPQPPEPLH TYLPGRLTCTCRRPGSPGGARGVVAGVRASLGL QCADLSCCLRGCLGCPSP*VE*SWARYAGLKAL QSPWWWYQ*MWKRRTCVSSRSASIATASILGKTS NWSNALSRRRSGRSSPPSC*AGGSGPTSGWLSA MG*G*ST*SPMRSTGCTHR*RWVRCRTSMSVCT WKPSGGMTFKSATCQKTSWRA*RRTGPRCSIFTN SSGTTTCSATPARSARAWPCSWAAWSSGGSSRI CPTMHLTRSPTSSS*KRKWGWTCFSQSRCRRT* SPNSSGR*SSRPSSSTPRSGRRSAS*SSSTLSPA SPTSTRRPTAVNSFKDGTLLWTWSLALKGSAS* LVRTQSPPAWPSSSRSGPSGASRWRRARQYFSW ALKVPPRPCPSKPHP*QRLRTWLTS*TATAGCRV STKALSSSILGKMVRSGTACPRSPC*TWRPGGP TSQRAAA*SQTSTQRFPTKPCEGPEVHSMALPV KMWS*IVFLGKAFLGRSMKVSTQITKGRKSM*LS RPARKTALWTTRRSS*ARQ*S*RTSTTRTS*S* SASLKRSPPGSSWNCIPMGSWATTWSGTRTP*R CSPSCCTHCRYAKPWPTWRASTACTGTLLSGTSW WPPLSV*SWGTLVFPGTLRTRTITKPL*LVSPS NGCPQSPLTSDASRQPVTSGCSPCACGRS*ALG SSPSSGWRTRMSSGCWRKETGCPSLISVHRSFIP S*PAAGTTTPVTGPASPSWCAASVTFIRWRRTL PWSKRGMLATEPPKSWSPQPSRNPHPSPADLST DPLRKPTSWLQSCSSRFLRVCVPALLRSPALWSI HLPLTHCTPHLSTGTMSSNATACGRRTSSNPAA EKRPSSCGRLKRSKCGKSWTNSRSRWWRTTSGS GRRRSPWTPWFI*MISPH*RQRRRSATWSSQGPH RSPRGWAHSPSSPQLTWTGLMTWCTSMSWSWCG PCWSSRMSSVSCPPRATWWW*RMWG*PCGSSSG AWMISCLPCRHLHGQRSRAPRNCSTKTWQSSSTR CGWHSRTP*PP*VRSARGRC*RLHTPWLWTPRT CSTLWTRPRFWPIWPTHLQSDGGWGPPACVFRP CLPCTSPALLLVMWVFQGEGQGESPSLATLHDAL SPPLPLAVLLRLQLDRGDSGLWTQGDGDKDGSE GDCCCLATAP*ASLVHAGGSWGWGGVTWCP*LY IWTWQADLGTKLFLSLPLRPSDVP*CTEKLGRSF VLGVRQPVR*GMGLAFLYSVY*NLFNVSLVWTD SMCPPEGGPGAQSRNKLIGSPGTGCCVVNKPSI RGKKQRDAAKIGPWAKSALFLPLFLFLPLLSLAF PSFLTPPLSLPNPHSHLHPSFLMCLHKHSFNFF LFDLWLN*NCPICF

Frame 1

TAALANRAGGMARGREGALGGAPPTSGVRGKSW ESGVGLLPAVPASSGPGGSLAGVARKGLPGGVV GLPCYWKPTSGCKWEKEPLP*PIPGNLRPADGRN QRCQLPASEVVAGS*STDVSALNSPGRQNSCLS KDLADLFPIIRDPCKGGVLLHLHLPL*L*VF*G LASHAELNRTSNFIDGCEDKLDGR*KFLAQSEHL INYMAGRAGTGLV*RAI*ARVIDLSLG*EWNIQ MEESSS*KDICSKAFFITVFSFF*SSHSLRSRC TPTCPADLPVLAAVPAHLAVPEE*SLESAPPWDC NVPILAAA*EDVWGVRAPESSKVGHVTPA*RPC RAHGGGTSRCGKGGRAYPQGLLL*QQLQSWEKL QTGQMHCPDGDPGDHHLHPAERADRAQHPVG*VL WAEAEAHEVR*DPLAAPTDDGG*GAGQV*VSAR GSRVEV*PSNPLLARRLHGEPEGGQDHAALFLP TAPERLHAALRQQGQRGHGPAAGLPGAQAVLQGY APQCT*QEVQLRAPRKGSGAGLVFPKADAGELK AQTVPEDDPADLPAVRLAQGGGVRHEVLQHSRR LRQHRPGDLPL*THSRMEHYCGPGHWP*RDPPAD *SGRKAHLPGRVQADQVHQVPPAGGGPGSTSAG H*RCPPGLVHQNLIPSRG*EHG*PHRRLLPAAG *APRLSHHPS*ERW*EAEQPAPDPHAKPGGPAVP PLRELQHRVRHLRRDSRRNPAKARRSTVWHCP* RCGPESYSWGRLFWGGL*RCLHKSQRGENQCSC QDLQERLHSGQQGEVHERGSDHEEPRPPAHREAD RHH*RGAHLDHHGIVSLWGAGPLPGAEQELPEG AHPRAVLTADMQSHGLPGEHQLRAQGHCCPEHP GGLP*VCEAGGLWSFPVH*GRGLLQSLCDSSPHQ MDVPRVH*LPTLHDSQ*RLDVRRVHVGDPELWE AALLLAGEQGCHRGAGERRPAAQA*SLSTGPLY PHDPLLGLRPQ*PAPLHRAGVQPQ*RLSDGEGHC HGAREECSLPNPQNLGAHSLPGTPTQAQPT*VQ TPSANQPPGSKAAVPGS*GSVCQLSYAHQPYGV SISR*LTAHPTSPPAQCLQTPQHAGGGLHPTQQP RRGPAAVGG*KGQNAANPGQTAEADGGGLPVAQ AGGEVPGPHGLYE**VPIDAREGGRLPGVHRAP TEAPEAGRTVHPAHS*PGPD**PGVPQCHGAGAG RAGAQE*ALSAAPRGLRGGGEECGADPAEAHRE RG*SPAFLAVIFTDRDRGHPETAQQRPGRAHQQ DAAGTAERRDLPK*GVQEADADGFTHPGCGRQEP ARRCGPGQGSGQSGPPTCRVTEGGGHLPASSAP ACHVPPLPCCWSCGSSRGKAKGSHLPLPLCTTP SPHPYPWLYCSGCSWTEGTLGYGHRVTVTKMAQR GTAAAWPLLPKPAWSMQGAPGGGEVSHGAPSFI YGHGRPIWEPSYSFPFLFGPQMSLDAQRSWGGA LFWGSGSQ*DEGWAWHSCTVYIEIYLM*VWSGLT ACALLREDLGHSPGTS*LGVQAQDAVLSTNQAS GGRSREMRPR*DLGPNPLSSCPSFSFFLYFPLL FPLFLLLLFLSPTPILICTLLFSCVCINILLTSF YLTCG*IKIVPFAL

Frame 2

QRRWPIVRGGWRGGGRGRSEEPHPLPVCAGNLG RAGWAFCPQFPPPQVRAGPWPG*HGRVSQAA** GFRVTGNLLPAANGKRSLYLNQSPGTSGPRMGE TRDANFLLPK*WLGLKAPMFLH*IALEDRILVSP KIWQTYSQL*EILAKEVSCFTFTFHCDCKFSEA SPAMRN*IGLATS*TVVKTS*TADESSWHRVNT **TIWQGGLERGLFEEQYEPGL*T*VWGKSGTSK WKSPAAERTFVQRPFSLQFSPSSDPATASGAVA HLPARPTYLYLPPSRLTWRCPRSSRWSPRLPGT AMCRS*LLPERMSGVSEPLSRVKLGTLRRPEGPA EPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNF KLVKCTVQTEIREIITSILLSGRIGPNIRLAEC YGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHV EAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQ QLRNDYMQRYASKVSEGMALQLGCLELRRFFKD MPHNALDKKSNFELLEKEVGLDLFFPKQMQENLK PKQFRKMIQQTFQQYASLREEECVMKFFNTLAG FANIDQETYRCELIQGWNITVDLVIGPKGIRQL TSQDAKPTCLAEFKQIRSIRCLPLEEGQAVLQLG IEGAPQALSIKTSSLAEAENMADLIDGYCRLQG EHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRS HLSESCSIESDIYAEIPDETLRRPGGPQYGIARE DVVLNRILGEGFFGEVYEGVYTNHKGEKINVAV KTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKL IGIIEEEPTWIIMELYPYGELGHYLERNKNSLKV LTLVLYSLQICKAMAYLESINCVHRDIAVRNIL VASPECVKLGDFGLSRYIEDEDYYKASVTRLPI KWMSPESINFRRFTTASDVWMFAVCMWEILSFGK QPFFWLENKDVIGVLEKGDRLPKPDLCPPVLYT LMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDI AMEQERNARYRTPKILEPTAFQEPPPKPSRPKYR PPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEY PSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSS REEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLR QEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPP QKPPRLGAQSIQPTANLDRTDDLVYLNVMELVR AVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGS VDDLLPSLPSSSRTEIEGTQKLLNKDLAELINK MRLAQQNAVTSLSEECKRQMLTASHTLAVDAKN LLDAVDQAKVLANLAHPPAE*RRVGATCLRLPPL PAMYLPCLAVGHVGLPGGRPRGVTFPCHFARRP LPTPTPGCTAQAAAGQRGLWAMDTG*R*QRWLR GGLLLPGHCSLSQPGPCRGLLGVGRCHMVPLALY MDMAGRFGNQAIPFPSSSALRCPLMHREAGEEL CFGGQAASEMRDGPGILVQCILKFI*CEFGLD* QHVPS*GRTWGTVQEQANWESRHRMLCCQQTKHQ GEEAERCGQDRTLGQIRSLPAPLSLSSFTFPCF SLFSYSSSFSPQPPFSSAPFFSHVFA*TFF*LL SI*LVVELKLSHLLC

Sequence PTK2B.aAug05

GACAGCGGCGCTGGCCAATCGTGCGGGGGGGATGGCGAGGGGGAGGGAGGGGGCGCTCGGAGGAGCCCCACCCACTTCCGGTGTGCGCGGGAAATCTTGG GAGAGCGGGGTGGGCCTTCTGCCCGCAGTTCCCGCCTCCTCAGGTCCGGGCGGGTCCCTGGCCGGGGTAGCACGGAAGGGTCTCCCAGGCGGCGTAGTAG GGCTTCCGTGTTACTGGAAACCTACTTCCGGCTGCAAATGGGAAAAGGAGCCTCTACCTTAACCAATCCCCGGGAACCTCAGGCCCGCGGATGGGAGAAA CCAGAGATGCCAACTTCCTGCTTCCGAAGTAGTGGCTGGGTCTTAAAGCACCGATGTTTCTGCATTGAATAGCCCTGGAAGACAGAATTCTTGTCTCTCC AAAGATCTGGCAGACTTATTCCCAATTATAAGAGATCCTTGCAAAGGAGGTGTCTTGCTTCACCTTCACCTTCCACTGTGATTGTAAGTTTTCTGAGGCC TCGCCAGCCATGCGGAACTGAATAGGACTAGCAACTTCATAGACGGTTGTGAAGACAAGCTAGACGGCAGATGAAAGTTCTTGGCACAGAGTGAACACTT GATAAACTATATGGCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGC CCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTC GAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGT CTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTG AGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGA CGGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAG CCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTG CAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGC TGGACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCGCTCAGGGA GGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACT GTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGCAGATCAGGTCCATCAGGT GCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAA CATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTG CCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCC TGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGT CTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTG ATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGC TGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTACCTGGAGAGCAT CAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCTGAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGAC GAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGACGTCTGGA TGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCG GCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTG TGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGAACCCCCAAAATCTTGGAGCCCACAGCCT TCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCT GTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTTAACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGC CACAGCATGCGGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACA AACAGCAGAAGCAGATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCATTGACGCC AGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACT GATGACCTGGTGTACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGG TGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAGGGCACCCA GAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATG CTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGT GACGGAGGGTGGGGGCCACCTGCCTGCGTCTTCCGCCCCTGCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCC AAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCACCCCTACCCCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGG GCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAGGGGGACTGCTGCTGCCTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCC TGGGGGTGGGGAGGTGTCACATGGTGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTTTCCCTTCCTCTTCGGCCCT CAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTGGGGGTCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGT ATATTGAAATTTATTTAATGTGAGTTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTAATTGGGAGT CCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCAGGGGGAAGAAGCAGAGAGATGCGGCCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCC TGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTTTCCCTCTTTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTC TTTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGGTTGAATTAAAATTGTCCCATTTGCTTTGC

PTK2B_x66_x147_f (forward primer)
PTK2B_x66_x147_r (reverse primer)
Single exon skipping

Frame 0

DSGAGQSCGGDGEGEGGGARRSPTHFRCAREIL GERGGPSARSSRLLRSGRVPGRGSTEGSPRRRSR ASVLLETYFRLQMGKGASTLTNPREPQARGWEK PEMPTSCFRSSGWVLKHRCFCIE*PWKTEFLSL QRSGRLIPNYKRSLQRRCLASPSPSTVIVSFLRP RQPCGTE*D*QLHRRL*RQARRQMKVLGTE*TL DKLYGSHSLRSRCTPTCPADLPVLAAVPAHLAV PEE*SLESAPPWDCNVPILAAA*EDVWGVRAPES SKVGHVTPA*RPCRAHGGGTSRCGKGGRAYPQG LLL*QQLQSWEKLQTGQMHCPDGDPGDHHLHPA ERADRAQHPVG*VLWAEAEAHEVR*DPLAAPTDD GG*GAGQV*VSARGSRVEV*PSNPLLARRLHGE PEGGQDHAALFLPTAPERLHAALRQQGQRGHGP AAGLPGAQAVLQGYAPQCT*QEVQLRAPRKGSGA GLVFPKADAGELKAQTVPEDDPADLPAVRLAQG GGVRHEVLQHSRRLRQHRPGDLPL*THSRMEHY CGPGHWP*RDPPAD*SGRKAHLPGRVQADQVHQV PPAGGGPGSTSAGH*RCPPGLVHQNLIPSRG*E HG*PHRRLLPAAG*APRLSHHPS*ERW*EAEQP APDPHAKPGGPAVPPLRELQHRVRHLRRDSRRNP AKARRSTVWHCP*RCGPESYSWGRLFWGGL*RC LHKSQRGENQCSCQDLQERLHSGQQGEVHERGS DHEEPRPPAHREADRHH*RGAHLDHHGIVSLWGA GPLPGAEQELPEGAHPRAVLTADMQSHGLPGEH QLRAQGHCCPEHPGGLP*VCEAGGLWSFPVH*G RGLLQSLCDSSPHQMDVPRVH*LPTLHDSQ*RLD VRRVHVGDPELWEAALLLAGEQGCHRGAGERRP AAQA*SLSTGPLYPHDPLLGLRPQ*PAPLHRAG VQPQ*RLSDGEGHCHGAREECSLPNPQNLGAHSL PGTPTQAQPT*VQTPSANQPPGSKAAVPGS*GS VCQLSYAHQPYGVSISR*LTAHPTSPPAQCLQT PQHAGGGLHPTQQPRRGPAAVGG*KGQNAANPGQ TAEADGGGLPVAQAGGEVPGPHGLYE**VPIDA REGGRLPGVHRAPTEAPEAGRTVHPAHS*PGPD **PGVPQCHGAGAGRAGAQE*ALSAAPRGLRGGG EECGADPAEAHRERG*SPAFLAVIFTDRDRGHP ETAQQRPGRAHQQDAAGTAERRDLPK*GVQEAD ADGFTHPGCGRQEPARRCGPGQGSGQSGPPTCRV TEGGGHLPASSAPACHVPPLPCCWSCGSSRGKA KGSHLPLPLCTTPSPHPYPWLYCSGCSWTEGTL GYGHRVTVTKMAQRGTAAAWPLLPKPAWSMQGAP GGGEVSHGAPSFIYGHGRPIWEPSYSFPFLFGP QMSLDAQRSWGGALFWGSGSQ*DEGWAWHSCTV YIEIYLM*VWSGLTACALLREDLGHSPGTS*LGV QAQDAVLSTNQASGGRSREMRPR*DLGPNPLSS CPSFSFFLYFPLLFPLFLLLLFLSPTPILICTL LFSCVCINILLTSFYLTCG*IKIVPFAL

Frame 1

TAALANRAGGMARGREGALGGAPPTSGVRGKSW ESGVGLLPAVPASSGPGGSLAGVARKGLPGGVV GLPCYWKPTSGCKWEKEPLP*PIPGNLRPADGRN QRCQLPASEVVAGS*STDVSALNSPGRQNSCLS KDLADLFPIIRDPCKGGVLLHLHLPL*L*VF*G LASHAELNRTSNFIDGCEDKLDGR*KFLAQSEHL INYMAATASGAVAHLPARPTYLYLPPSRLTWRC PRSSRWSPRLPGTAMCRS*LLPERMSGVSEPLS RVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKV CFYSNSFNPGKNFKLVKCTVQTEIREIITSILL SGRIGPNIRLAECYGLRLKHMKSDEIHWLHPQM TVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMES LKEDRTTLLYFYQQLRNDYMQRYASKVSEGMAL QLGCLELRRFFKDMPHNALDKKSNFELLEKEVG LDLFFPKQMQENLKPKQFRKMIQQTFQQYASLRE EECVMKFFNTLAGFANIDQETYRCELIQGWNIT VDLVIGPKGIRQLTSQDAKPTCLAEFKQIRSIR CLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAEN MADLIDGYCRLQGEHQGSLIIHPRKDGEKRNSL PQIPMLNLEARRSHLSESCSIESDIYAEIPDET LRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGV YTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAV IMKNLDHPHIVKLIGIIEEEPTWIIMELYPYGE LGHYLERNKNSLKVLTLVLYSLQICKAMAYLESI NCVHRDIAVRNILVASPECVKLGDFGLSRYIED EDYYKASVTRLPIKWMSPESINFRRFTTASDVW MFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDR LPKPDLCPPVLYTLMTRCWDYDPSDRPRFTELV CSLSDVYQMEKDIAMEQERNARYRTPKILEPTA FQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGL CASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKR HSMREEDFIQPSSREEAQQLWEAEKVKMRQILD KQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTP EKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRT DDLVYLNVMELVRAVLELKNELCQLPPEGYVVV VKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQ KLLNKDLAELINKMRLAQQNAVTSLSEECKRQM LTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE *RRVGATCLRLPPLPAMYLPCLAVGHVGLPGGRP RGVTFPCHFARRPLPTPTPGCTAQAAAGQRGLW AMDTG*R*QRWLRGGLLLPGHCSLSQPGPCRGL LGVGRCHMVPLALYMDMAGRFGNQAIPFPSSSAL RCPLMHREAGEELCFGGQAASEMRDGPGILVQC ILKFI*CEFGLD*QHVPS*GRTWGTVQEQANWE SRHRMLCCQQTKHQGEEAERCGQDRTLGQIRSLP APLSLSSFTFPCFSLFSYSSSFSPQPPFSSAPF FSHVFA*TFF*LLSI*LVVELKLSHLLC

Frame 2

QRRWPIVRGGWRGGGRGRSEEPHPLPVCAGNLG RAGWAFCPQFPPPQVRAGPWPG*HGRVSQAA** GFRVTGNLLPAANGKRSLYLNQSPGTSGPRMGE TRDANFLLPK*WLGLKAPMFLH*IALEDRILVSP KIWQTYSQL*EILAKEVSCFTFTFHCDCKFSEA SPAMRN*IGLATS*TVVKTS*TADESSWHRVNT **TIWQPQPPEPLHTYLPGRLTCTCRRPGSPGGA RGVVAGVRASLGLQCADLSCCLRGCLGCPSP*V E*SWARYAGLKALQSPWWWYQ*MWKRRTCVSSR SASIATASILGKTSNWSNALSRRRSGRSSPPSC* AGGSGPTSGWLSAMG*G*ST*SPMRSTGCTHR* RWVRCRTSMSVCTWKPSGGMTFKSATCQKTSWR A*RRTGPRCSIFTNSSGTTTCSATPARSARAWPC SWAAWSSGGSSRICPTMHLTRSPTSSS*KRKWG WTCFSQSRCRRT*SPNSSGR*SSRPSSSTPRSG RRSAS*SSSTLSPASPTSTRRPTAVNSFKDGTLL WTWSLALKGSAS*LVRTQSPPAWPSSSRSGPSG ASRWRRARQYFSWALKVPPRPCPSKPHP*QRLR TWLTS*TATAGCRVSTKALSSSILGKMVRSGTAC PRSPC*TWRPGGPTSQRAAA*SQTSTQRFPTKP CEGPEVHSMALPVKMWS*IVFLGKAFLGRSMKV STQITKGRKSM*LSRPARKTALWTTRRSS*ARQ* S*RTSTTRTS*S*SASLKRSPPGSSWNCIPMGS WATTWSGTRTP*RCSPSCCTHCRYAKPWPTWRA STACTGTLLSGTSWWPPLSV*SWGTLVFPGTLRT RTITKPL*LVSPSNGCPQSPLTSDASRQPVTSG CSPCACGRS*ALGSSPSSGWRTRMSSGCWRKET GCPSLISVHRSFIPS*PAAGTTTPVTGPASPSWC AASVTFIRWRRTLPWSKRGMLATEPPKSWSPQP SRNPHPSPADLSTDPLRKPTSWLQSCSSRFLRV CVPALLRSPALWSIHLPLTHCTPHLSTGTMSSNA TACGRRTSSNPAAEKRPSSCGRLKRSKCGKSWT NSRSRWWRTTSGSGRRRSPWTPWFI*MISPH*R QRRRSATWSSQGPHRSPRGWAHSPSSPQLTWTGL MTWCTSMSWSWCGPCWSSRMSSVSCPPRATWWW *RMWG*PCGSSSGAWMISCLPCRHLHGQRSRAP RNCSTKTWQSSSTRCGWHSRTP*PP*VRSARGRC *RLHTPWLWTPRTCSTLWTRPRFWPIWPTHLQS DGGWGPPACVFRPCLPCTSPALLLVMWVFQGEG QGESPSLATLHDALSPPLPLAVLLRLQLDRGDSG LWTQGDGDKDGSEGDCCCLATAP*ASLVHAGGS WGWGGVTWCP*LYIWTWQADLGTKLFLSLPLRP SDVP*CTEKLGRSFVLGVRQPVR*GMGLAFLYSV Y*NLFNVSLVWTDSMCPPEGGPGAQSRNKLIGS PGTGCCVVNKPSIRGKKQRDAAKIGPWAKSALF LPLFLFLPLLSLAFPSFLTPPLSLPNPHSHLHPS FLMCLHKHSFNFFLFDLWLN*NCPICF