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Sequences

Sequence AXL.cAug05

AGAGGGACAGCGCCCAGAGCCCGGATAGAGAGACACGGCCTCACTGGCTCAGGACAGGGGGCACAGCCACCAGGGTCCCCTTCCCCCTCCTCAGCTCCCT CCCTGGCCCCTTTAAGAAAGAGCTGATCCTCTCCTCTCTTGAGTTAACCCCTGATTGTCCAGGTGGCCCCTGGGCTCTGGCCCTGGTGGGCGGAGGCAAA GGGGGAGCCAGGGGCGGAGAAAGGGTTGCCCAAGTCTGGGAGTGAGGGAAGGAGGCAGGGGTGCTGAGAAGGCGGCTGCTGGGCAGAGCCGGTGGCAAGG GCCTCCCCTGCCGCTGTGCCAGGCAGGCAGTGCCAAATCCGGGGAGCCTGGAGCTGGGGGGAGGGCCGGGGACAGCCCGGCCCTGCCCCCTCCCCCGCTG GGAGCCCAACAACTTCTGAGGAAAGTTTGGCACCCATGGCGTGGCGGTGCCCCAGGATGGGCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTG GGCGTGCATGGCCCCCAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGGGAATATCACAGGTGCCCGGGGACTCACGGGCACCCTTCGG TGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATCCTGGAGCTCGCGGACAGCACCCAGACCCAGGTGCCCCTGG GTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCTGCAGCTTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGG ACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTGGGCTGGAGGGCTTGCCTTACTTCCTGGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCC TTCAACCTGAGCTGCCAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAGGATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGGCCCCC AGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATAACGCCAAGGGGGTCACCACATCCCGCACAGCCACCATCAC AGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAGCTGGAGGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTG ACCCACTGCACCCTGCAGGCTGTGCTGTCAAACGATGGGATGGGCATCCAGGCGGGAGAACCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCG TGCCCCCCCATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCGTGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGGACCCA CTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGCTACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAG CCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGACACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGG TGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCCTACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACC CCTGGAGGCCTGGCGCCCAGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTACTGCTAGGAGCAGTCGTGGCCGCTGCCTGTGTC CTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAGACCCGTTATGGAGAAGTGTTTGAACCAACAGTGGAAAGAGGTGAACTGGTAGTCAGGT ACCGCGTGCGCAAGTCCTACAGTCGTCGGACCACTGAAGCTACCTTGAACAGCCTGGGCATCAGTGAAGAGCTGAAGGAGAAGCTGCGGGATGTGATGGT GGACCGGCACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGAGCTGTGATGGAAGGCCAGCTCAACCAGGACGACTCCATCCTCAAGGTG GCTGTGAAGACGATGAAGATTGCCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGTCTGCATGAAGGAATTTGACCATCCCAACGTCA TGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCCCAGCACCTGTGGTCATCTTACCTTTCATGAAACATGGAGACCTACACAGCTT CCTCCTCTATTCCCGGCTCGGGGACCAGCCAGTGTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGACATCGCCAGTGGCATGGAGTATCTGAGT ACCAAGAGATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGGCGGACTTCGGGCTCTCCAAGAAGATCT ACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGCCAGTCAAGTGGATTGCCATTGAGAGTCTAGCTGACCGTGTCTACACCAGCAAGAGCGA TGTGTGGTCCTTCGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCAAACCCCATATCCGGGCGTGGAGAACAGCGAGATTTATGACTATCTGCGCCAG GGAAATCGCCTGAAGCAGCCTGCGGACTGTCTGGATGGACTGTATGCCTTGATGTCGCGGTGCTGGGAGCTAAATCCCCAGGACCGGCCAAGTTTTACAG AGCTGCGGGAAGATTTGGAGAACACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGTCAACATGGATGAGGGTGGAGGTTATCC TGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTCCTGTAGCTGCCTCACTGCGGCTGAGGTCCATCCTGCTGGA CGCTATGTCCTCTGCCCTTCCACAACCCCTAGCCCCGCTCAGCCTGCTGATAGGGGCTCCCCAGCAGCCCCAGGGCAGGAGGATGGTGCCTGAGACAACC CTCCACCTGGTACTCCCTCTCAGGATCCAAGCTAAGCACTGCCACTGGGGAAAACTCCACCTTCCCACTTTCCCACCCCACGCCTTATCCCCACTTGCAG CCCTGTCTTCCTACCTATCCCACCTCCATCCCAGACAGGTCCCTCCCCTTCTCTGTGCAGTAGCATCACCTTGAAAGCAGTAGCATCACCATCTGTAAAA GGAAGGGGTTGGATTGCAATATCTGAAGCCCTCCCAGGTGTTAACATTCCAAGACTCTAGAGTCCAAGGTTTAAAGAGTCTAGATTCAAAGGTTCTAGGT TTCAAAGATGCTGTGAGTCTTTGGTTCTAAGGACCTGAAATTCCAAAGTCTCTAATTCTATTAAAGTGCTAAGGTTCTAAGGCCTACTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTGCGATAGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCTCGCCTCACTGCAACCTTCACCTACCGAGTTCA AGTGATTTTCCTGCCTTGGCCTCCCAAGTAGCTGGGATTACAGGTGTGTGCCACCACACCCGGCTAATTTTTATATTTTTAGTAGAGACAGGGTTTCACC ATGTTGGCCAGGCTGGTCTAAAACTCCTGACCTCAAGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGAGATTACAGGCATGAGCCACTGCACTCAACC TTAAGACCTACTGTTCTAAAGCTCTGACATTATGTGGTTTTAGATTTTCTGGTTCTAACATTTTTGATAAAGCCTCAAGGTTTTAGGTTCTAAAGTTCTA AGATTCTGATTTTAGGAGCTAAGGCTCTATGAGTCTAGATGTTTATTCTTCTAGAGTTCAGAGTCCTTAAAATGTAAGATTATAGATTCTAAAGATTCTA TAGTTCTAGACATGGAGGTTCTAAGGCCTAGGATTCTAAAATGTGATGTTCTAAGGCTCTGAGAGTCTAGATTCTCTGGCTGTAAGGCTCTAGATCATAA GGCTTCAAAATGTTATCTTCTCAAGTTCTAAGATTCTAATGATGATCAATTATAGTTTCTGAGGCTTTATGATAATAGATTCTCTTGTATAAGATCCTAG ATCCTAAGGGTCGAAAGCTCTAGAATCTGCAATTCAAAAGTTCCAAGAGTCTAAAGATGGAGTTTCTAAGGTCCGGTGTTCTAAGATGTGATATTCTAAG ACTTACTCTAAGATCTTAGATTCTCTGTGTCTAAGATTCTAGATCAGATGCTCCAAGATTCTAGATGATTAAATAAGATTCTAACGGTCTGTTCTGTTTC AAGGCACTCTAGATTCCATTGGTCCAAGATTCCGGATCCTAAGCATCTAAGTTATAAGACTCTCACACTCAGTTGTGACTAACTAGACACCAAAGTTCTA ATAATTTCTAATGTTGGACACCTTTAGGTTCTTTGCTGCATTCTGCCTCTCTAGGACCATGGTTAAGAGTCCAAGAATCCACATTTCTAAAATCTTATAG TTCTAGGCACTGTAGTTCTAAGACTCAAATGTTCTAAGTTTCTAAGATTCTAAAGGTCCACAGGTCTAGACTATTAGGTGCAATTTCAAGGTTCTAACCC TATACTGTAGTATTCTTTGGGGTGCCCCTCTCCTTCTTAGCTATCATTGCTTCCTCCTCCCCAACTGTGGGGGTGTGCCCCCTTCAAGCCTGTGCAATGC ATTAGGGATGCCTCCTTTCCCGCAGGGGATGGACGATCTCCCACCTTTCGGGCCATGTTGCCCCCGTGAGCCAATCCCTCACCTTCTGAGTACAGAGTGT GGACTCTGGTGCCTCCAGAGGGGCTCAGGTCACATAAAACTTTGTATATCAACGAG

AXL_x151_x133_f (forward primer)
AXL_x151_x133_r (reverse primer)
Single exon skipping

Frame 0

RGTAPRARIERHGLTGSGQGAQPPGSPSPSSAP SLAPLRKS*SSPLLS*PLIVQVAPGLWPWWAEAK GEPGAEKGLPKSGSEGRRQGC*EGGCWAEPVAR ASPAAVPGRQCQIRGAWSWGEGRGQPGPAPSPA GSPTTSEESLAPMAWRCPRMGRVPLAWCLALCGW ACMAPRGTQAEESPFVGNPGNITGARGLTGTLR CQLQVQGEPPEVHWLRDGQILELADSTQTQVPL GEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLG HQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTP FNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGP QRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATIT VLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPL THCTLQAVLSNDGMGIQAGEPDPPEEPLTSQAS VPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTH WLPVETPEGVPLGPPENISATRNGSQAFVHWQE PRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQE VTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVP LEAWRPVKEPSTPAFSWPWWYVLLGAVVAAACV LILALFLVHRRKKETRYGEVFEPTVERGELVVR YRVRKSYSRRTTEATLNSLGISEELKEKLRDVMV DRHKVALGKTLGEGEFGAVMEGQLNQDDSILKV AVKTMKIAICTRSELEDFLSEAVCMKEFDHPNV MRLIGVCFQGSERESFPAPVVILPFMKHGDLHSF LLYSRLGDQPVYLPTQMLVKFMADIASGMEYLS TKRFIHRDLAARNCMLNENMSVCVADFGLSKKI YNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSD VWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQ GNRLKQPADCLDGLYALMSRCWELNPQDRPSFT ELREDLENTLKALPPAQEPDEILYVNMDEGGGYP EPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAG RYVLCPSTTPSPAQPADRGSPAAPGQEDGA*DN PPPGTPSQDPS*ALPLGKTPPSHFPTPRLIPTCS PVFLPIPPPSQTGPSPSLCSSITLKAVASPSVK GRGWIAISEALPGVNIPRL*SPRFKESRFKGSR FQRCCESLVLRT*NSKVSNSIKVLRF*GLLFFFF FFFFFLR*SLTVSPRLECSGAISPHCNLHLPSS SDFPALASQVAGITGVCHHTRLIFIFLVETGFH HVGQAGLKLLTSSDLPTSASQSAEITGMSHCTQP *DLLF*SSDIMWF*IFWF*HF**SLKVLGSKVL RF*F*ELRLYESRCLFF*SSESLKCKIIDSKDS IVLDMEVLRPRILKCDVLRL*ESRFSGCKALDHK ASKCYLLKF*DSNDDQL*FLRLYDNRFSCIRS* ILRVESSRICNSKVPRV*RWSF*GPVF*DVIF* DLL*DLRFSVSKILDQMLQDSR*LNKILTVCSVS RHSRFHWSKIPDPKHLSYKTLTLSCD*LDTKVL IISNVGHL*VLCCILPL*DHG*ESKNPHF*NLI VLGTVVLRLKCSKFLRF*RSTGLDY*VQFQGSNP IL*YSLGCPSPS*LSLLPPPQLWGCAPFKPVQC IRDASFPAGDGRSPTFRAMLPP*ANPSPSEYRV WTLVPPEGLRSHKTLYINE

Frame 1

EGQRPEPG*RDTASLAQDRGHSHQGPLPPPQLP PWPL*ERADPLLS*VNP*LSRWPLGSGPGGRRQ RGSQGRRKGCPSLGVREGGRGAEKAAAGQSRWQG PPLPLCQAGSAKSGEPGAGGRAGDSPALPPPPL GAQQLLRKVWHPWRGGAPGWAGSRWPGAWRCAA GRAWPPGARRLKKVPSWATQGISQVPGDSRAPFG VSSRFRESPPRYIGFGMDRSWSSRTAPRPRCPW VRMNRMTG*WSASSESPPCSFPTRDSTSVWCFW DIRPSCPSLAMLGWRACLTSWRSPKTGLWPPTPP ST*AAKLRDPQSPWTYSGSRMLSPWPRLQVTAP SAACMFQG*TRHPLSPAKPITPRGSPHPAQPPS QCSPSSPVTSTWSPANPRSWRWLGLQA*AASTP* PTAPCRLCCQTMGWASRRENQTPQRSPSPRKHP CPPISFG*AASILTPLITSAWHAPAARAPHPGP TGFLWRRRRECPWAPLRTLVLRGMGARPSCIGKS PGRPCRVPC*GTGWRIKARTPQRC*WT*G*GKR *PWSCRGTGLCPI*QCVWQPTLLLGMDPGASQY PWRPGAQ*RNLQLLPSRGPGGMYC*EQSWPLPVS SSWLSSLSTGERRRPVMEKCLNQQWKEVNW*SG TACASPTVVGPLKLP*TAWASVKS*RRSCGM*W WTGTRWPWGRLWERESLEL*WKASSTRTTPSSRW L*RR*RLPSARGQSWRIS*VKRSA*RNLTIPTS *GSSVSVSRVLNERASQHLWSSYLS*NMETYTA SSSIPGSGTSQCTCPLRC**SSWQTSPVAWSI*V PRDSYTGTWRPGTAC*MRTCPCVWRTSGSPRRS TMGTTTARDVSPRCQSSGLPLRV*LTVSTPARA MCGPSG*QCGRLPQEAKPHIRAWRTARFMTICAR EIA*SSLRTVWMDCMP*CRGAGS*IPRTGQVLQ SCGKIWRTH*RPCLLPRSLTKSSMSTWMRVEVI LNPLELQEELTPQPSQTLRIPVAASLRLRSILLD AMSSALPQPLAPLSLLIGAPQQPQGRRMVPETT LHLVLPLRIQAKHCHWGKLHLPTFPPHALSPLA ALSSYLSHLHPRQVPPLLCAVASP*KQ*HHHL*K EGVGLQYLKPSQVLTFQDSRVQGLKSLDSKVLG FKDAVSLWF*GPEIPKSLILLKC*GSKAYFFFF FFFFFFCDRVSLCHPGWSAVVQSRLTATFTYRVQ VIFLPWPPK*LGLQVCATTPG*FLYF**RQGFT MLARLV*NS*PQVICPPQPPKVLRLQA*ATALN LKTYCSKALTLCGFRFSGSNIFDKASRF*VLKF* DSDFRS*GSMSLDVYSSRVQSP*NVRL*ILKIL *F*TWRF*GLGF*NVMF*GSESLDSLAVRL*II RLQNVIFSSSKILMMINYSF*GFMIIDSLV*DPR S*GSKALESAIQKFQESKDGVSKVRCSKM*YSK TYSKILDSLCLRF*IRCSKILDD*IRF*RSVLF QGTLDSIGPRFRILSI*VIRLSHSVVTN*TPKF* *FLMLDTFRFFAAFCLSRTMVKSPRIHISKIL* F*AL*F*DSNVLSF*DSKGPQV*TIRCNFKVLT LYCSILWGAPLLLSYHCFLLPNCGGVPPSSLCNA LGMPPFPQGMDDLPPFGPCCPREPIPHLLSTEC GLWCLQRGSGHIKLCIST

Frame 2

RDSAQSPDRETRPHWLRTGGTATRVPFPLLSSL PGPFKKELILSSLELTPDCPGGPWALALVGGGK GGARGGERVAQVWE*GKEAGVLRRRLLGRAGGK GLPCRCARQAVPNPGSLELGGGPGTARPCPLPRW EPNNF*GKFGTHGVAVPQDGQGPAGLVLGAVRL GVHGPQGHAG*RKSLRGQPREYHRCPGTHGHPS VSAPGSGRAPRGTLASGWTDPGARGQHPDPGAPG *G*TG*LDSGQPAQNHLPAAFRHGTVPVFGVSG TSDLRVPAWLCWAGGLALLPGGARRQDCGRQHP LQPELPSSGTPRARGPTLAPGCCPPGHGSRSRPP AQPACSRAEQDILFLLRSP*RQGGHHIPHSHHH SAPPAAP*PPPGLPPTHGAGGGLDSRPERHLPP DPLHPAGCAVKRWDGHPGGRTRPPRGAPHLASIR APPSASARQPPSSHPLSHPRGMHQQPGPLILDP LASCGDAGGSAPGPP*EH*CYAEWEPGLRALAR APGAPAGYPVRVPAGVSRPGHPRGANGHRAKARG DPGAAGGRVCVQSDSVCGSLHCCWGWTLEPPST PGGLAPSEGTFNSCLLVALVVCTARSSRGRCLC PHLGSLPCPPAKEGDPLWRSV*TNSGKR*TGSQV PRAQVLQSSDH*SYLEQPGHQ*RAEGEAAGCDG GPAQGGPGEDSGRGRVWSCDGRPAQPGRLHPQG GCEDDEDCHLHEVRAGGFPE*SGLHEGI*PSQRH EAHRCLFPGF*TRELPSTCGHLTFHETWRPTQL PPLFPARGPASVPAHSDASEVHGRHRQWHGVSE YQEIHTPGPGGQELHAE*EHVRVCGGLRALQEDL QWGLLPPGTYRQDASQVDCH*ESS*PCLHQQER CVVLRGDNVGDCHKRPNPISGRGEQRDL*LSAP GKSPEAACGLSGWTVCLDVAVLGAKSPGPAKFYR AAGRFGEHTEGLASCPGA*RNPLCQHG*GWRLS *TPWSCRRS*PPNPARP*GFL*LPHCG*GPSCW TLCPLPFHNP*PRSAC**GLPSSPRAGGWCLRQP STWYSLSGSKLSTATGENSTFPLSHPTPYPHLQ PCLPTYPTSIPDRSLPFSVQ*HHLESSSITICK RKGLDCNI*SPPRC*HSKTLESKV*RV*IQRF*V SKML*VFGSKDLKFQSL*FY*SAKVLRPTFFFF FFFFFFAIESHCVTQAGVQWCNLASLQPSPTEF K*FSCLGLPSSWDYRCVPPHPANFYIFSRDRVSP CWPGWSKTPDLK*SAHLSLPKC*DYRHEPLHST LRPTVLKL*HYVVLDFLVLTFLIKPQGFRF*SS KILILGAKAL*V*MFILLEFRVLKM*DYRF*RFY SSRHGGSKA*DSKM*CSKALRV*ILWL*GSRS* GFKMLSSQVLRF***SIIVSEAL***ILLYKIL DPKGRKL*NLQFKSSKSLKMEFLRSGVLRCDILR LTLRS*ILCV*DSRSDAPRF*MIK*DSNGLFCF KAL*IPLVQDSGS*ASKL*DSHTQL*LTRHQSS NNF*CWTPLGSLLHSASLGPWLRVQESTFLKSYS SRHCSSKTQMF*VSKILKVHRSRLLGAISRF*P YTVVFFGVPLSFLAIIASSSPTVGVCPLQACAM H*GCLLSRRGWTISHLSGHVAPVSQSLTF*VQSV DSGASRGAQVT*NFVYQR

Sequence AXL.bAug05

AGAGGGACAGCGCCCAGAGCCCGGATAGAGAGACACGGCCTCACTGGCTCAGGACAGGGGGCACAGCCACCAGGGTCCCCTTCCCCCTCCTCAGCTCCCT CCCTGGCCCCTTTAAGAAAGAGCTGATCCTCTCCTCTCTTGAGTTAACCCCTGATTGTCCAGGTGGCCCCTGGGCTCTGGCCCTGGTGGGCGGAGGCAAA GGGGGAGCCAGGGGCGGAGAAAGGGTTGCCCAAGTCTGGGAGTGAGGGAAGGAGGCAGGGGTGCTGAGAAGGCGGCTGCTGGGCAGAGCCGGTGGCAAGG GCCTCCCCTGCCGCTGTGCCAGGCAGGCAGTGCCAAATCCGGGGAGCCTGGAGCTGGGGGGAGGGCCGGGGACAGCCCGGCCCTGCCCCCTCCCCCGCTG GGAGCCCAACAACTTCTGAGGAAAGTTTGGCACCCATGGCGTGGCGGTGCCCCAGGATGGGCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTG GGCGTGCATGGCCCCCAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGGGAATATCACAGGTGCCCGGGGACTCACGGGCACCCTTCGG TGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATCCTGGAGCTCGCGGACAGCACCCAGACCCAGGTGCCCCTGG GTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCTGCAGCTTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGG ACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTGGGCTGGAGGGCTTGCCTTACTTCCTGGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCC TTCAACCTGAGCTGCCAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAGGATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGGCCCCC AGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATAACGCCAAGGGGGTCACCACATCCCGCACAGCCACCATCAC AGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAGCTGGAGGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTG ACCCACTGCACCCTGCAGGCTGTGCTGTCAAACGATGGGATGGGCATCCAGGCGGGAGAACCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCG TGCCCCCCCATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCGTGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGGACCCA CTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGCTACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAG CCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGACACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGG TGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCCTACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACC CCTGGAGGCCTGGCGCCCAGGGCAAGCACAGCCAGTCCACCAGCTGGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTACTGCTA GGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAGACCCGTTATGGAGAAGTGTTTGAACCAACAG TGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCAAGTCCTACAGTCGTCGGACCACTGAAGCTACCTTGAACAGCCTGGGCATCAGTGAAGAGCT GAAGGAGAAGCTGCGGGATGTGATGGTGGACCGGCACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGAGCTGTGATGGAAGGCCAGCTC AACCAGGACGACTCCATCCTCAAGGTGGCTGTGAAGACGATGAAGATTGCCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGTCTGCA TGAAGGAATTTGACCATCCCAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCCCAGCACCTGTGGTCATCTTACCTTT CATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAGCCAGTGTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGAC ATCGCCAGTGGCATGGAGTATCTGAGTACCAAGAGATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGG CGGACTTCGGGCTCTCCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGCCAGTCAAGTGGATTGCCATTGAGAGTCTAGC TGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCAAACCCCATATCCGGGCGTGGAGAAC AGCGAGATTTATGACTATCTGCGCCAGGGAAATCGCCTGAAGCAGCCTGCGGACTGTCTGGATGGACTGTATGCCTTGATGTCGCGGTGCTGGGAGCTAA ATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGGGAAGATTTGGAGAACACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGT CAACATGGATGAGGGTGGAGGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTCCTGTAGCTGCCTC ACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAGCCCCGCTCAGCCTGCTGATAGGGGCTCCCCAGCAGCCCCAG GGCAGGAGGATGGTGCCTGAGACAACCCTCCACCTGGTACTCCCTCTCAGGATCCAAGCTAAGCACTGCCACTGGGGAAAACTCCACCTTCCCACTTTCC CACCCCACGCCTTATCCCCACTTGCAGCCCTGTCTTCCTACCTATCCCACCTCCATCCCAGACAGGTCCCTCCCCTTCTCTGTGCAGTAGCATCACCTTG AAAGCAGTAGCATCACCATCTGTAAAAGGAAGGGGTTGGATTGCAATATCTGAAGCCCTCCCAGGTGTTAACATTCCAAGACTCTAGAGTCCAAGGTTTA AAGAGTCTAGATTCAAAGGTTCTAGGTTTCAAAGATGCTGTGAGTCTTTGGTTCTAAGGACCTGAAATTCCAAAGTCTCTAATTCTATTAAAGTGCTAAG GTTCTAAGGCCTAGGATTCTAAAATGTGATGTTCTAAGGCTCTGAGAGTCTAGATTCTCTGGCTGTAAGGCTCTAGATCATAAGGCTTCAAAATGTTATC TTCTCAAGTTCTAAGATTCTAATGATGATCAATTATAGTTTCTGAGGCTTTATGATAATAGATTCTCTTGTATAAGATCCTAGATCCTAAGGGTCGAAAG CTCTAGAATCTGCAATTCAAAAGTTCCAAGAGTCTAAAGATGGAGTTTCTAAGGTCCGGTGTTCTAAGATGTGATATTCTAAGACTTACTCTAAGATCTT AGATTCTCTGTGTCTAAGATTCTAGATCAGATGCTCCAAGATTCTAGATGATTAAATAAGATTCTAACGGTCTGTTCTGTTTCAAGGCACTCTAGATTCC ATTGGTCCAAGATTCCGGATCCTAAGCATCTAAGTTATAAGACTCTCACACTCAGTTGTGACTAACTAGACACCAAAGTTCTAATAATTTCTAATGTTGG ACACCTTTAGGTTCTTTGCTGCATTCTGCCTCTCTAGGACCATGGTTAAGAGTCCAAGAATCCACATTTCTAAAATCTTATAGTTCTAGGCACTGTAGTT CTAAGACTCAAATGTTCTAAGTTTCTAAGATTCTAAAGGTCCACAGGTCTAGACTATTAGGTGCAATTTCAAGGTTCTAACCCTATACTGTAGTATTCTT TGGGGTGCCCCTCTCCTTCTTAGCTATCATTGCTTCCTCCTCCCCAACTGTGGGGGTGTGCCCCCTTCAAGCCTGTGCAATGCATTAGGGATGCCTCCTT TCCCGCAGGGGATGGACGATCTCCCACCTTTCGGGCCATGTTGCCCCCGTGAGCCAATCCCTCACCTTCTGAGTACAGAGTGTGGACTCTGGTGCCTCCA GAGGGGCTCAGGTCACATAAAACTTTGTATATCAACGAG

AXL_x151_x133_f (forward primer)
AXL_x151_x133_r (reverse primer)
Single exon skipping

Frame 0

RGTAPRARIERHGLTGSGQGAQPPGSPSPSSAP SLAPLRKS*SSPLLS*PLIVQVAPGLWPWWAEAK GEPGAEKGLPKSGSEGRRQGC*EGGCWAEPVAR ASPAAVPGRQCQIRGAWSWGEGRGQPGPAPSPA GSPTTSEESLAPMAWRCPRMGRVPLAWCLALCGW ACMAPRGTQAEESPFVGNPGNITGARGLTGTLR CQLQVQGEPPEVHWLRDGQILELADSTQTQVPL GEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLG HQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTP FNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGP QRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATIT VLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPL THCTLQAVLSNDGMGIQAGEPDPPEEPLTSQAS VPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTH WLPVETPEGVPLGPPENISATRNGSQAFVHWQE PRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQE VTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVP LEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLL GAVVAAACVLILALFLVHRRKKETRYGEVFEPT VERGELVVRYRVRKSYSRRTTEATLNSLGISEEL KEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQL NQDDSILKVAVKTMKIAICTRSELEDFLSEAVC MKEFDHPNVMRLIGVCFQGSERESFPAPVVILPF MKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMAD IASGMEYLSTKRFIHRDLAARNCMLNENMSVCV ADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLA DRVYTSKSDVWSFGVTMWEIATRGQTPYPGVEN SEIYDYLRQGNRLKQPADCLDGLYALMSRCWEL NPQDRPSFTELREDLENTLKALPPAQEPDEILYV NMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCL TAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAP GQEDGA*DNPPPGTPSQDPS*ALPLGKTPPSHFP TPRLIPTCSPVFLPIPPPSQTGPSPSLCSSITL KAVASPSVKGRGWIAISEALPGVNIPRL*SPRF KESRFKGSRFQRCCESLVLRT*NSKVSNSIKVLR F*GLGF*NVMF*GSESLDSLAVRL*IIRLQNVI FSSSKILMMINYSF*GFMIIDSLV*DPRS*GSK ALESAIQKFQESKDGVSKVRCSKM*YSKTYSKIL DSLCLRF*IRCSKILDD*IRF*RSVLFQGTLDS IGPRFRILSI*VIRLSHSVVTN*TPKF**FLML DTFRFFAAFCLSRTMVKSPRIHISKIL*F*AL*F *DSNVLSF*DSKGPQV*TIRCNFKVLTLYCSIL WGAPLLLSYHCFLLPNCGGVPPSSLCNALGMPP FPQGMDDLPPFGPCCPREPIPHLLSTECGLWCLQ RGSGHIKLCIST

Frame 1

EGQRPEPG*RDTASLAQDRGHSHQGPLPPPQLP PWPL*ERADPLLS*VNP*LSRWPLGSGPGGRRQ RGSQGRRKGCPSLGVREGGRGAEKAAAGQSRWQG PPLPLCQAGSAKSGEPGAGGRAGDSPALPPPPL GAQQLLRKVWHPWRGGAPGWAGSRWPGAWRCAA GRAWPPGARRLKKVPSWATQGISQVPGDSRAPFG VSSRFRESPPRYIGFGMDRSWSSRTAPRPRCPW VRMNRMTG*WSASSESPPCSFPTRDSTSVWCFW DIRPSCPSLAMLGWRACLTSWRSPKTGLWPPTPP ST*AAKLRDPQSPWTYSGSRMLSPWPRLQVTAP SAACMFQG*TRHPLSPAKPITPRGSPHPAQPPS QCSPSSPVTSTWSPANPRSWRWLGLQA*AASTP* PTAPCRLCCQTMGWASRRENQTPQRSPSPRKHP CPPISFG*AASILTPLITSAWHAPAARAPHPGP TGFLWRRRRECPWAPLRTLVLRGMGARPSCIGKS PGRPCRVPC*GTGWRIKARTPQRC*WT*G*GKR *PWSCRGTGLCPI*QCVWQPTLLLGMDPGASQY PWRPGAQGKHSQSTSW*RNLQLLPSRGPGGMYC* EQSWPLPVSSSWLSSLSTGERRRPVMEKCLNQQ WKEVNW*SGTACASPTVVGPLKLP*TAWASVKS *RRSCGM*WWTGTRWPWGRLWERESLEL*WKASS TRTTPSSRWL*RR*RLPSARGQSWRIS*VKRSA *RNLTIPTS*GSSVSVSRVLNERASQHLWSSYL S*NMETYTASSSIPGSGTSQCTCPLRC**SSWQT SPVAWSI*VPRDSYTGTWRPGTAC*MRTCPCVW RTSGSPRRSTMGTTTARDVSPRCQSSGLPLRV* LTVSTPARAMCGPSG*QCGRLPQEAKPHIRAWRT ARFMTICAREIA*SSLRTVWMDCMP*CRGAGS* IPRTGQVLQSCGKIWRTH*RPCLLPRSLTKSSM STWMRVEVILNPLELQEELTPQPSQTLRIPVAAS LRLRSILLDAMSSALPQPLAPLSLLIGAPQQPQ GRRMVPETTLHLVLPLRIQAKHCHWGKLHLPTF PPHALSPLAALSSYLSHLHPRQVPPLLCAVASP* KQ*HHHL*KEGVGLQYLKPSQVLTFQDSRVQGL KSLDSKVLGFKDAVSLWF*GPEIPKSLILLKC* GSKA*DSKM*CSKALRV*ILWL*GSRS*GFKMLS SQVLRF***SIIVSEAL***ILLYKILDPKGRK L*NLQFKSSKSLKMEFLRSGVLRCDILRLTLRS *ILCV*DSRSDAPRF*MIK*DSNGLFCFKAL*IP LVQDSGS*ASKL*DSHTQL*LTRHQSSNNF*CW TPLGSLLHSASLGPWLRVQESTFLKSYSSRHCS SKTQMF*VSKILKVHRSRLLGAISRF*PYTVVFF GVPLSFLAIIASSSPTVGVCPLQACAMH*GCLL SRRGWTISHLSGHVAPVSQSLTF*VQSVDSGAS RGAQVT*NFVYQR

Frame 2

RDSAQSPDRETRPHWLRTGGTATRVPFPLLSSL PGPFKKELILSSLELTPDCPGGPWALALVGGGK GGARGGERVAQVWE*GKEAGVLRRRLLGRAGGK GLPCRCARQAVPNPGSLELGGGPGTARPCPLPRW EPNNF*GKFGTHGVAVPQDGQGPAGLVLGAVRL GVHGPQGHAG*RKSLRGQPREYHRCPGTHGHPS VSAPGSGRAPRGTLASGWTDPGARGQHPDPGAPG *G*TG*LDSGQPAQNHLPAAFRHGTVPVFGVSG TSDLRVPAWLCWAGGLALLPGGARRQDCGRQHP LQPELPSSGTPRARGPTLAPGCCPPGHGSRSRPP AQPACSRAEQDILFLLRSP*RQGGHHIPHSHHH SAPPAAP*PPPGLPPTHGAGGGLDSRPERHLPP DPLHPAGCAVKRWDGHPGGRTRPPRGAPHLASIR APPSASARQPPSSHPLSHPRGMHQQPGPLILDP LASCGDAGGSAPGPP*EH*CYAEWEPGLRALAR APGAPAGYPVRVPAGVSRPGHPRGANGHRAKARG DPGAAGGRVCVQSDSVCGSLHCCWGWTLEPPST PGGLAPRASTASPPAGEGTFNSCLLVALVVCTA RSSRGRCLCPHLGSLPCPPAKEGDPLWRSV*TNS GKR*TGSQVPRAQVLQSSDH*SYLEQPGHQ*RA EGEAAGCDGGPAQGGPGEDSGRGRVWSCDGRPA QPGRLHPQGGCEDDEDCHLHEVRAGGFPE*SGLH EGI*PSQRHEAHRCLFPGF*TRELPSTCGHLTF HETWRPTQLPPLFPARGPASVPAHSDASEVHGR HRQWHGVSEYQEIHTPGPGGQELHAE*EHVRVCG GLRALQEDLQWGLLPPGTYRQDASQVDCH*ESS *PCLHQQERCVVLRGDNVGDCHKRPNPISGRGE QRDL*LSAPGKSPEAACGLSGWTVCLDVAVLGAK SPGPAKFYRAAGRFGEHTEGLASCPGA*RNPLC QHG*GWRLS*TPWSCRRS*PPNPARP*GFL*LP HCG*GPSCWTLCPLPFHNP*PRSAC**GLPSSPR AGGWCLRQPSTWYSLSGSKLSTATGENSTFPLS HPTPYPHLQPCLPTYPTSIPDRSLPFSVQ*HHL ESSSITICKRKGLDCNI*SPPRC*HSKTLESKV* RV*IQRF*VSKML*VFGSKDLKFQSL*FY*SAK VLRPRILKCDVLRL*ESRFSGCKALDHKASKCY LLKF*DSNDDQL*FLRLYDNRFSCIRS*ILRVES SRICNSKVPRV*RWSF*GPVF*DVIF*DLL*DL RFSVSKILDQMLQDSR*LNKILTVCSVSRHSRF HWSKIPDPKHLSYKTLTLSCD*LDTKVLIISNVG HL*VLCCILPL*DHG*ESKNPHF*NLIVLGTVV LRLKCSKFLRF*RSTGLDY*VQFQGSNPIL*YS LGCPSPS*LSLLPPPQLWGCAPFKPVQCIRDASF PAGDGRSPTFRAMLPP*ANPSPSEYRVWTLVPP EGLRSHKTLYINE

Sequence AXL.aAug05

AGAGGGACAGCGCCCAGAGCCCGGATAGAGAGACACGGCCTCACTGGCTCAGGACAGGGGGCACAGCCACCAGGGTCCCCTTCCCCCTCCTCAGCTCCCT CCCTGGCCCCTTTAAGAAAGAGCTGATCCTCTCCTCTCTTGAGTTAACCCCTGATTGTCCAGGTGGCCCCTGGGCTCTGGCCCTGGTGGGCGGAGGCAAA GGGGGAGCCAGGGGCGGAGAAAGGGTTGCCCAAGTCTGGGAGTGAGGGAAGGAGGCAGGGGTGCTGAGAAGGCGGCTGCTGGGCAGAGCCGGTGGCAAGG GCCTCCCCTGCCGCTGTGCCAGGCAGGCAGTGCCAAATCCGGGGAGCCTGGAGCTGGGGGGAGGGCCGGGGACAGCCCGGCCCTGCCCCCTCCCCCGCTG GGAGCCCAACAACTTCTGAGGAAAGTTTGGCACCCATGGCGTGGCGGTGCCCCAGGATGGGCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTG GGCGTGCATGGCCCCCAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGGGAATATCACAGGTGCCCGGGGACTCACGGGCACCCTTCGG TGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATCCTGGAGCTCGCGGACAGCACCCAGACCCAGGTGCCCCTGG GTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCTGCAGCTTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGG ACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTGGGCTGGAGGGCTTGCCTTACTTCCTGGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCC TTCAACCTGAGCTGCCAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAGGATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGGCCCCC AGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATAACGCCAAGGGGGTCACCACATCCCGCACAGCCACCATCAC AGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAGCTGGAGGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTG ACCCACTGCACCCTGCAGGCTGTGCTGTCAAACGATGGGATGGGCATCCAGGCGGGAGAACCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCG TGCCCCCCCATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCGTGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGGACCCA CTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGCTACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAG CCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGACACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGG TGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCCTACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACC CCTGGAGGCCTGGCGCCCAGGGCAAGCACAGCCAGTCCACCAGCTGGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTACTGCTA GGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAGACCCGTTATGGAGAAGTGTTTGAACCAACAG TGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCAAGTCCTACAGTCGTCGGACCACTGAAGCTACCTTGAACAGCCTGGGCATCAGTGAAGAGCT GAAGGAGAAGCTGCGGGATGTGATGGTGGACCGGCACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGAGCTGTGATGGAAGGCCAGCTC AACCAGGACGACTCCATCCTCAAGGTGGCTGTGAAGACGATGAAGATTGCCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGTCTGCA TGAAGGAATTTGACCATCCCAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCCCAGCACCTGTGGTCATCTTACCTTT CATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAGCCAGTGTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGAC ATCGCCAGTGGCATGGAGTATCTGAGTACCAAGAGATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGG CGGACTTCGGGCTCTCCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGCCAGTCAAGTGGATTGCCATTGAGAGTCTAGC TGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCAAACCCCATATCCGGGCGTGGAGAAC AGCGAGATTTATGACTATCTGCGCCAGGGAAATCGCCTGAAGCAGCCTGCGGACTGTCTGGATGGACTGTATGCCTTGATGTCGCGGTGCTGGGAGCTAA ATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGGGAAGATTTGGAGAACACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGT CAACATGGATGAGGGTGGAGGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTCCTGTAGCTGCCTC ACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAGCCCCGCTCAGCCTGCTGATAGGGGCTCCCCAGCAGCCCCAG GGCAGGAGGATGGTGCCTGAGACAACCCTCCACCTGGTACTCCCTCTCAGGATCCAAGCTAAGCACTGCCACTGGGGAAAACTCCACCTTCCCACTTTCC CACCCCACGCCTTATCCCCACTTGCAGCCCTGTCTTCCTACCTATCCCACCTCCATCCCAGACAGGTCCCTCCCCTTCTCTGTGCAGTAGCATCACCTTG AAAGCAGTAGCATCACCATCTGTAAAAGGAAGGGGTTGGATTGCAATATCTGAAGCCCTCCCAGGTGTTAACATTCCAAGACTCTAGAGTCCAAGGTTTA AAGAGTCTAGATTCAAAGGTTCTAGGTTTCAAAGATGCTGTGAGTCTTTGGTTCTAAGGACCTGAAATTCCAAAGTCTCTAATTCTATTAAAGTGCTAAG GTTCTAAGGCCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGATAGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCTCGCCT CACTGCAACCTTCACCTACCGAGTTCAAGTGATTTTCCTGCCTTGGCCTCCCAAGTAGCTGGGATTACAGGTGTGTGCCACCACACCCGGCTAATTTTTA TATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTAAAACTCCTGACCTCAAGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGAGAT TACAGGCATGAGCCACTGCACTCAACCTTAAGACCTACTGTTCTAAAGCTCTGACATTATGTGGTTTTAGATTTTCTGGTTCTAACATTTTTGATAAAGC CTCAAGGTTTTAGGTTCTAAAGTTCTAAGATTCTGATTTTAGGAGCTAAGGCTCTATGAGTCTAGATGTTTATTCTTCTAGAGTTCAGAGTCCTTAAAAT GTAAGATTATAGATTCTAAAGATTCTATAGTTCTAGACATGGAGGTTCTAAGGCCTAGGATTCTAAAATGTGATGTTCTAAGGCTCTGAGAGTCTAGATT CTCTGGCTGTAAGGCTCTAGATCATAAGGCTTCAAAATGTTATCTTCTCAAGTTCTAAGATTCTAATGATGATCAATTATAGTTTCTGAGGCTTTATGAT AATAGATTCTCTTGTATAAGATCCTAGATCCTAAGGGTCGAAAGCTCTAGAATCTGCAATTCAAAAGTTCCAAGAGTCTAAAGATGGAGTTTCTAAGGTC CGGTGTTCTAAGATGTGATATTCTAAGACTTACTCTAAGATCTTAGATTCTCTGTGTCTAAGATTCTAGATCAGATGCTCCAAGATTCTAGATGATTAAA TAAGATTCTAACGGTCTGTTCTGTTTCAAGGCACTCTAGATTCCATTGGTCCAAGATTCCGGATCCTAAGCATCTAAGTTATAAGACTCTCACACTCAGT TGTGACTAACTAGACACCAAAGTTCTAATAATTTCTAATGTTGGACACCTTTAGGTTCTTTGCTGCATTCTGCCTCTCTAGGACCATGGTTAAGAGTCCA AGAATCCACATTTCTAAAATCTTATAGTTCTAGGCACTGTAGTTCTAAGACTCAAATGTTCTAAGTTTCTAAGATTCTAAAGGTCCACAGGTCTAGACTA TTAGGTGCAATTTCAAGGTTCTAACCCTATACTGTAGTATTCTTTGGGGTGCCCCTCTCCTTCTTAGCTATCATTGCTTCCTCCTCCCCAACTGTGGGGG TGTGCCCCCTTCAAGCCTGTGCAATGCATTAGGGATGCCTCCTTTCCCGCAGGGGATGGACGATCTCCCACCTTTCGGGCCATGTTGCCCCCGTGAGCCA ATCCCTCACCTTCTGAGTACAGAGTGTGGACTCTGGTGCCTCCAGAGGGGCTCAGGTCACATAAAACTTTGTATATCAACGAG

AXL_x151_x133_f (forward primer)
AXL_x151_x133_r (reverse primer)
Single exon skipping

Frame 0

RGTAPRARIERHGLTGSGQGAQPPGSPSPSSAP SLAPLRKS*SSPLLS*PLIVQVAPGLWPWWAEAK GEPGAEKGLPKSGSEGRRQGC*EGGCWAEPVAR ASPAAVPGRQCQIRGAWSWGEGRGQPGPAPSPA GSPTTSEESLAPMAWRCPRMGRVPLAWCLALCGW ACMAPRGTQAEESPFVGNPGNITGARGLTGTLR CQLQVQGEPPEVHWLRDGQILELADSTQTQVPL GEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLG HQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTP FNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGP QRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATIT VLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPL THCTLQAVLSNDGMGIQAGEPDPPEEPLTSQAS VPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTH WLPVETPEGVPLGPPENISATRNGSQAFVHWQE PRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQE VTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVP LEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLL GAVVAAACVLILALFLVHRRKKETRYGEVFEPT VERGELVVRYRVRKSYSRRTTEATLNSLGISEEL KEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQL NQDDSILKVAVKTMKIAICTRSELEDFLSEAVC MKEFDHPNVMRLIGVCFQGSERESFPAPVVILPF MKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMAD IASGMEYLSTKRFIHRDLAARNCMLNENMSVCV ADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLA DRVYTSKSDVWSFGVTMWEIATRGQTPYPGVEN SEIYDYLRQGNRLKQPADCLDGLYALMSRCWEL NPQDRPSFTELREDLENTLKALPPAQEPDEILYV NMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCL TAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAP GQEDGA*DNPPPGTPSQDPS*ALPLGKTPPSHFP TPRLIPTCSPVFLPIPPPSQTGPSPSLCSSITL KAVASPSVKGRGWIAISEALPGVNIPRL*SPRF KESRFKGSRFQRCCESLVLRT*NSKVSNSIKVLR F*GLLFFFFFFFFFLR*SLTVSPRLECSGAISP HCNLHLPSSSDFPALASQVAGITGVCHHTRLIF IFLVETGFHHVGQAGLKLLTSSDLPTSASQSAEI TGMSHCTQP*DLLF*SSDIMWF*IFWF*HF**S LKVLGSKVLRF*F*ELRLYESRCLFF*SSESLK CKIIDSKDSIVLDMEVLRPRILKCDVLRL*ESRF SGCKALDHKASKCYLLKF*DSNDDQL*FLRLYD NRFSCIRS*ILRVESSRICNSKVPRV*RWSF*G PVF*DVIF*DLL*DLRFSVSKILDQMLQDSR*LN KILTVCSVSRHSRFHWSKIPDPKHLSYKTLTLS CD*LDTKVLIISNVGHL*VLCCILPL*DHG*ES KNPHF*NLIVLGTVVLRLKCSKFLRF*RSTGLDY *VQFQGSNPIL*YSLGCPSPS*LSLLPPPQLWG CAPFKPVQCIRDASFPAGDGRSPTFRAMLPP*A NPSPSEYRVWTLVPPEGLRSHKTLYINE

Frame 1

EGQRPEPG*RDTASLAQDRGHSHQGPLPPPQLP PWPL*ERADPLLS*VNP*LSRWPLGSGPGGRRQ RGSQGRRKGCPSLGVREGGRGAEKAAAGQSRWQG PPLPLCQAGSAKSGEPGAGGRAGDSPALPPPPL GAQQLLRKVWHPWRGGAPGWAGSRWPGAWRCAA GRAWPPGARRLKKVPSWATQGISQVPGDSRAPFG VSSRFRESPPRYIGFGMDRSWSSRTAPRPRCPW VRMNRMTG*WSASSESPPCSFPTRDSTSVWCFW DIRPSCPSLAMLGWRACLTSWRSPKTGLWPPTPP ST*AAKLRDPQSPWTYSGSRMLSPWPRLQVTAP SAACMFQG*TRHPLSPAKPITPRGSPHPAQPPS QCSPSSPVTSTWSPANPRSWRWLGLQA*AASTP* PTAPCRLCCQTMGWASRRENQTPQRSPSPRKHP CPPISFG*AASILTPLITSAWHAPAARAPHPGP TGFLWRRRRECPWAPLRTLVLRGMGARPSCIGKS PGRPCRVPC*GTGWRIKARTPQRC*WT*G*GKR *PWSCRGTGLCPI*QCVWQPTLLLGMDPGASQY PWRPGAQGKHSQSTSW*RNLQLLPSRGPGGMYC* EQSWPLPVSSSWLSSLSTGERRRPVMEKCLNQQ WKEVNW*SGTACASPTVVGPLKLP*TAWASVKS *RRSCGM*WWTGTRWPWGRLWERESLEL*WKASS TRTTPSSRWL*RR*RLPSARGQSWRIS*VKRSA *RNLTIPTS*GSSVSVSRVLNERASQHLWSSYL S*NMETYTASSSIPGSGTSQCTCPLRC**SSWQT SPVAWSI*VPRDSYTGTWRPGTAC*MRTCPCVW RTSGSPRRSTMGTTTARDVSPRCQSSGLPLRV* LTVSTPARAMCGPSG*QCGRLPQEAKPHIRAWRT ARFMTICAREIA*SSLRTVWMDCMP*CRGAGS* IPRTGQVLQSCGKIWRTH*RPCLLPRSLTKSSM STWMRVEVILNPLELQEELTPQPSQTLRIPVAAS LRLRSILLDAMSSALPQPLAPLSLLIGAPQQPQ GRRMVPETTLHLVLPLRIQAKHCHWGKLHLPTF PPHALSPLAALSSYLSHLHPRQVPPLLCAVASP* KQ*HHHL*KEGVGLQYLKPSQVLTFQDSRVQGL KSLDSKVLGFKDAVSLWF*GPEIPKSLILLKC* GSKAYFFFFFFFFFFCDRVSLCHPGWSAVVQSRL TATFTYRVQVIFLPWPPK*LGLQVCATTPG*FL YF**RQGFTMLARLV*NS*PQVICPPQPPKVLR LQA*ATALNLKTYCSKALTLCGFRFSGSNIFDKA SRF*VLKF*DSDFRS*GSMSLDVYSSRVQSP*N VRL*ILKIL*F*TWRF*GLGF*NVMF*GSESLD SLAVRL*IIRLQNVIFSSSKILMMINYSF*GFMI IDSLV*DPRS*GSKALESAIQKFQESKDGVSKV RCSKM*YSKTYSKILDSLCLRF*IRCSKILDD* IRF*RSVLFQGTLDSIGPRFRILSI*VIRLSHSV VTN*TPKF**FLMLDTFRFFAAFCLSRTMVKSP RIHISKIL*F*AL*F*DSNVLSF*DSKGPQV*T IRCNFKVLTLYCSILWGAPLLLSYHCFLLPNCGG VPPSSLCNALGMPPFPQGMDDLPPFGPCCPREP IPHLLSTECGLWCLQRGSGHIKLCIST

Frame 2

RDSAQSPDRETRPHWLRTGGTATRVPFPLLSSL PGPFKKELILSSLELTPDCPGGPWALALVGGGK GGARGGERVAQVWE*GKEAGVLRRRLLGRAGGK GLPCRCARQAVPNPGSLELGGGPGTARPCPLPRW EPNNF*GKFGTHGVAVPQDGQGPAGLVLGAVRL GVHGPQGHAG*RKSLRGQPREYHRCPGTHGHPS VSAPGSGRAPRGTLASGWTDPGARGQHPDPGAPG *G*TG*LDSGQPAQNHLPAAFRHGTVPVFGVSG TSDLRVPAWLCWAGGLALLPGGARRQDCGRQHP LQPELPSSGTPRARGPTLAPGCCPPGHGSRSRPP AQPACSRAEQDILFLLRSP*RQGGHHIPHSHHH SAPPAAP*PPPGLPPTHGAGGGLDSRPERHLPP DPLHPAGCAVKRWDGHPGGRTRPPRGAPHLASIR APPSASARQPPSSHPLSHPRGMHQQPGPLILDP LASCGDAGGSAPGPP*EH*CYAEWEPGLRALAR APGAPAGYPVRVPAGVSRPGHPRGANGHRAKARG DPGAAGGRVCVQSDSVCGSLHCCWGWTLEPPST PGGLAPRASTASPPAGEGTFNSCLLVALVVCTA RSSRGRCLCPHLGSLPCPPAKEGDPLWRSV*TNS GKR*TGSQVPRAQVLQSSDH*SYLEQPGHQ*RA EGEAAGCDGGPAQGGPGEDSGRGRVWSCDGRPA QPGRLHPQGGCEDDEDCHLHEVRAGGFPE*SGLH EGI*PSQRHEAHRCLFPGF*TRELPSTCGHLTF HETWRPTQLPPLFPARGPASVPAHSDASEVHGR HRQWHGVSEYQEIHTPGPGGQELHAE*EHVRVCG GLRALQEDLQWGLLPPGTYRQDASQVDCH*ESS *PCLHQQERCVVLRGDNVGDCHKRPNPISGRGE QRDL*LSAPGKSPEAACGLSGWTVCLDVAVLGAK SPGPAKFYRAAGRFGEHTEGLASCPGA*RNPLC QHG*GWRLS*TPWSCRRS*PPNPARP*GFL*LP HCG*GPSCWTLCPLPFHNP*PRSAC**GLPSSPR AGGWCLRQPSTWYSLSGSKLSTATGENSTFPLS HPTPYPHLQPCLPTYPTSIPDRSLPFSVQ*HHL ESSSITICKRKGLDCNI*SPPRC*HSKTLESKV* RV*IQRF*VSKML*VFGSKDLKFQSL*FY*SAK VLRPTFFFFFFFFFFAIESHCVTQAGVQWCNLA SLQPSPTEFK*FSCLGLPSSWDYRCVPPHPANFY IFSRDRVSPCWPGWSKTPDLK*SAHLSLPKC*D YRHEPLHSTLRPTVLKL*HYVVLDFLVLTFLIK PQGFRF*SSKILILGAKAL*V*MFILLEFRVLKM *DYRF*RFYSSRHGGSKA*DSKM*CSKALRV*I LWL*GSRS*GFKMLSSQVLRF***SIIVSEAL* **ILLYKILDPKGRKL*NLQFKSSKSLKMEFLRS GVLRCDILRLTLRS*ILCV*DSRSDAPRF*MIK *DSNGLFCFKAL*IPLVQDSGS*ASKL*DSHTQ L*LTRHQSSNNF*CWTPLGSLLHSASLGPWLRVQ ESTFLKSYSSRHCSSKTQMF*VSKILKVHRSRL LGAISRF*PYTVVFFGVPLSFLAIIASSSPTVG VCPLQACAMH*GCLLSRRGWTISHLSGHVAPVSQ SLTF*VQSVDSGASRGAQVT*NFVYQR