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Sequences

Sequence RTN4.hAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACG CTCCTCTGCAGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTC AGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAG ATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTG CACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTG TTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGAC TTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGG AGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGAT CTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTGACTGCCATGTGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAA ACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATAAAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG TTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAA ATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGCTTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATT GCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAGCTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGT CATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGAAATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATA GAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGC AAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAGATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTG TGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Single exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSG*DPFCSSCCI*ACDT LLCSC*PPVLERH*EDWSGVWCQPIPAAFIDSI QHCERNSLHCLGPALCDHQL*DIQGCDPSYPEIR *RPPIQGISGI*SCYI*GVGSEVQ*FCSWSCEL HDKGTQAPLLS**FS*FSEVCSVDVGIYLCWCL V*WSDTTDFGSHFTLQCSCYL*TASGTDRSLSRT CK*EC*RCYG*NPSKNPWIEAQS*MKTPKIISR SSSLKGIFI*LYGGGSGKNEP*RCSAVSQIVVR SLFLAMHCCEEKLPVLTAMCSSS*VL*AAMYGFK P*SYLFPIYLRHWWNKKPVYFTLLQIVLPHLGK LQRWWS*KKKKKSPFQFVHCVWSV*IDADFLK* NVCLDEIIPVKQE*QSLLFWYVLGVL*LLLLY*L PI*VNIDYICIVFHKA*TFTFQPPHSA*YFRVS HWLYMCSSKAHKLEEEIFLGALPSVFNMKCHTH RTPTSISLHRLTVVNFVTESMD*I*CFQKCCLFA NIKHCYARNY*LQNEDLYHCGLSCTELNLWNAL *TVKAKYQ*SL*T*NML

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVDETLFALPAASEPVIR SSAVVDLLYWRDIKKTGVVFGASLFLLLSLTVF SIVSVTAYIALALLSVTISFRIYKGVIQAIQKS DEGHPFRAYLESEVAISEELVQKYSNSALGHVNC TIKELRRLFLVDDLVDSLKFAVLMWVFTYVGAL FNGLTLLILALISLFSVPVIYERHQAQIDHYLG LANKNVKDAMAKIQAKIPGLKRKAE*KRPK*LVG VHL*RGYSFDYTGEGQGRTNLDVAVQFHRSLLD LYF*PCTVVRKNYLS*LPCVHHLKYCKLLCMDL NRNHIFFLSI*GTGGIKNLYILLCCR*SCRILAS CRDGGARKKKKKALFSLCTVYGPCRLMQIF*NE MFV*TRSYR*SRNDKACFSGMF*VYCDFYCYIN CQYK*I*IIYV*CFTKLRPLPSSHPTVLDISESV IGYTCVVPKHIS*KKKYF*EHYHLFST*NATHI ELQHQFHCTD*L*LILSQNLWTESNASKNVVCL QISNIVMQEIINYKMKIYTIVV*AVLN*ICGMHC EL*KQSINKAYRLKIC

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWMRPFLLFLLHLSL*Y APLQLLTSCTGETLRRLEWCLVPAYSCCFH*QYS AL*A*QPTLPWPCSL*PSALGYTRV*SKLSRNQ MKATHSGHIWNLKLLYLRSWFRSTVILLLVM*T AR*RNSGASS*LMI*LIL*SLQC*CGYLPMLVPC LMV*HY*FWLSFHSSVFLLFMNGIRHR*III*D LQIRMLKMLWLKSKQKSLD*SAKLNENAQNN** EFIFKGDIHLIIRGRVREERTLTLQCSFTDRC*I FIFSHALL*GKITCLDCHVFIILSIVSCYVWI* TVIISFSYLSEALVE*KTCIFYFVADSLAASWQ VAEMVELEKKKKKPFSVCALCMVRVD*CRFSEMK CLFRRDHTGKAGMTKLAFLVCSRCIVTFTVILI ANISKYRLYMYSVSQSLDLYLPATPQCLIFQSQ SLVIHV*FQST*ARRRNISRSTTICFQHEMPHT* NSNINFIAQTDCS*FCHRIYGLNLMLPKMLFVC KYQTLLCKKLLITK*RFIPLWFKLY*TKSVECI VNCKSKVSIKLIDLKYA

Sequence RTN4.kAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACG CTCCTCTGCAGAAAATATGGACTTGAAGGAGCAGCCAGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTGCTTGAAACTGCTGCTTCT CTTCCTTCTCTGTCTCCTCTCTCAGCCGCTTCTTTCAAAGAACATGAATACCTTGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGG TGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAG CTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAG TTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGA AGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGT TATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGA TTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAA GCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGA ATGACAAAGCTTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTG TTTCACAAAGCTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGC TAGAAGAAGAAATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGT AGTTAATTTTGTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTA CAAAATGAAGATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGA CTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Single exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSG*DPFCSSCCI*ACDT LLCRKYGLEGAAR*HYFGWSRGFPICPA*NCCF SSFSVSSLSRFFQRT*IPCC*PPVLERH*EDWSG VWCQPIPAAFIDSIQHCERNSLHCLGPALCDHQ L*DIQGCDPSYPEIR*RPPIQGISGI*SCYI*G VGSEVQ*FCSWSCELHDKGTQAPLLS**FS*FSE VCSVDVGIYLCWCLV*WSDTTDFGSHFTLQCSC YL*TASGTDRSLSRTCK*EC*RCYG*NPSKNPW IEAQS*MKTPKIINSLAASWQVAEMVELEKKKKK PFSVCALCMVRVD*CRFSEMKCLFRRDHTGKAG MTKLAFLVCSRCIVTFTVILIANISKYRLYMYS VSQSLDLYLPATPQCLIFQSQSLVIHV*FQST*A RRRNISRSTTICFQHEMPHT*NSNINFIAQTDC S*FCHRIYGLNLMLPKMLFVCKYQTLLCKKLLI TK*RFIPLWFKLY*TKSVECIVNCKSKVSIKLID LKYA

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVDETLFALPAASEPVIR SSAENMDLKEQPGNTISAGQEDFPSVLLETAAS LPSLSPLSAASFKEHEYLVVDLLYWRDIKKTGV VFGASLFLLLSLTVFSIVSVTAYIALALLSVTIS FRIYKGVIQAIQKSDEGHPFRAYLESEVAISEE LVQKYSNSALGHVNCTIKELRRLFLVDDLVDSL KFAVLMWVFTYVGALFNGLTLLILALISLFSVPV IYERHQAQIDHYLGLANKNVKDAMAKIQAKIPG LKRKAE*KRPK*LIVLPHLGKLQRWWS*KKKKK SPFQFVHCVWSV*IDADFLK*NVCLDEIIPVKQE *QSLLFWYVLGVL*LLLLY*LPI*VNIDYICIV FHKA*TFTFQPPHSA*YFRVSHWLYMCSSKAHK LEEEIFLGALPSVFNMKCHTHRTPTSISLHRLTV VNFVTESMD*I*CFQKCCLFANIKHCYARNY*L QNEDLYHCGLSCTELNLWNAL*TVKAKYQ*SL* T*NML

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWMRPFLLFLLHLSL*Y APLQKIWT*RSSQVTLFRLVKRISHLSCLKLLLL FLLCLLSQPLLSKNMNTLLLTSCTGETLRRLEW CLVPAYSCCFH*QYSAL*A*QPTLPWPCSL*PS ALGYTRV*SKLSRNQMKATHSGHIWNLKLLYLRS WFRSTVILLLVM*TAR*RNSGASS*LMI*LIL* SLQC*CGYLPMLVPCLMV*HY*FWLSFHSSVFL LFMNGIRHR*III*DLQIRMLKMLWLKSKQKSLD *SAKLNENAQNN**SCRILASCRDGGARKKKKK ALFSLCTVYGPCRLMQIF*NEMFV*TRSYR*SR NDKACFSGMF*VYCDFYCYINCQYK*I*IIYV*C FTKLRPLPSSHPTVLDISESVIGYTCVVPKHIS *KKKYF*EHYHLFST*NATHIELQHQFHCTD*L *LILSQNLWTESNASKNVVCLQISNIVMQEIINY KMKIYTIVV*AVLN*ICGMHCEL*KQSINKAYR LKIC