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Sequences

Sequence RTN4.iAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGT GTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGC TTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGT TGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAA GTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTT ATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGAT TGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTCAGGGAAGAAC GAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGATCTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTGACTGCCATGT GTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATAAAAAACCTGT ATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCAC TGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGCTTGCTTTTCT GGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAGCTTAGACCTT TACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGAAATATTTCTA GGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAAT CTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAGATTTATACCA TTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Multiple exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSGC*PPVLERH*EDWSG VWCQPIPAAFIDSIQHCERNSLHCLGPALCDHQ L*DIQGCDPSYPEIR*RPPIQGISGI*SCYI*GV GSEVQ*FCSWSCELHDKGTQAPLLS**FS*FSE VCSVDVGIYLCWCLV*WSDTTDFGSHFTLQCSC YL*TASGTDRSLSRTCK*EC*RCYG*NPSKNPWI EAQS*MKTPKIISRSSSLKGIFI*LYGGGSGKN EP*RCSAVSQIVVRSLFLAMHCCEEKLPVLTAM CSSS*VL*AAMYGFKP*SYLFPIYLRHWWNKKPV YFTLLQIVLPHLGKLQRWWS*KKKKKSPFQFVH CVWSV*IDADFLK*NVCLDEIIPVKQE*QSLLF WYVLGVL*LLLLY*LPI*VNIDYICIVFHKA*TF TFQPPHSA*YFRVSHWLYMCSSKAHKLEEEIFL GALPSVFNMKCHTHRTPTSISLHRLTVVNFVTE SMD*I*CFQKCCLFANIKHCYARNY*LQNEDLYH CGLSCTELNLWNAL*TVKAKYQ*SL*T*NML

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVVVDLLYWRDIKKTGVV FGASLFLLLSLTVFSIVSVTAYIALALLSVTIS FRIYKGVIQAIQKSDEGHPFRAYLESEVAISEE LVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLK FAVLMWVFTYVGALFNGLTLLILALISLFSVPV IYERHQAQIDHYLGLANKNVKDAMAKIQAKIPG LKRKAE*KRPK*LVGVHL*RGYSFDYTGEGQGRT NLDVAVQFHRSLLDLYF*PCTVVRKNYLS*LPC VHHLKYCKLLCMDLNRNHIFFLSI*GTGGIKNL YILLCCR*SCRILASCRDGGARKKKKKALFSLCT VYGPCRLMQIF*NEMFV*TRSYR*SRNDKACFS GMF*VYCDFYCYINCQYK*I*IIYV*CFTKLRP LPSSHPTVLDISESVIGYTCVVPKHIS*KKKYF* EHYHLFST*NATHIELQHQFHCTD*L*LILSQN LWTESNASKNVVCLQISNIVMQEIINYKMKIYT IVV*AVLN*ICGMHCEL*KQSINKAYRLKIC

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWLLTSCTGETLRRLEW CLVPAYSCCFH*QYSAL*A*QPTLPWPCSL*PSA LGYTRV*SKLSRNQMKATHSGHIWNLKLLYLRS WFRSTVILLLVM*TAR*RNSGASS*LMI*LIL* SLQC*CGYLPMLVPCLMV*HY*FWLSFHSSVFLL FMNGIRHR*III*DLQIRMLKMLWLKSKQKSLD *SAKLNENAQNN**EFIFKGDIHLIIRGRVREE RTLTLQCSFTDRC*IFIFSHALL*GKITCLDCHV FIILSIVSCYVWI*TVIISFSYLSEALVE*KTC IFYFVADSLAASWQVAEMVELEKKKKKPFSVCA LCMVRVD*CRFSEMKCLFRRDHTGKAGMTKLAFL VCSRCIVTFTVILIANISKYRLYMYSVSQSLDL YLPATPQCLIFQSQSLVIHV*FQST*ARRRNIS RSTTICFQHEMPHT*NSNINFIAQTDCS*FCHRI YGLNLMLPKMLFVCKYQTLLCKKLLITK*RFIP LWFKLY*TKSVECIVNCKSKVSIKLIDLKYA

Sequence RTN4.jAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACGAGGAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCC GCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTC CCCCCGTCGCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTC CAAGCTCCCTGAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCG GCTCCCGCCGCGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGA CTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGT GACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATA TCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTG ATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAG TGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAA ATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTC AGGGAAGAACGAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGATCTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTG ACTGCCATGTGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATA AAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCA GTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGC TTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAG CTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGA AATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTT GTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAG ATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATAT GCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Multiple exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRRGGRRGGGRGGRGRRPGGAGGAGEEARRRAV RGPSAHRPCRRRAPDGLRK*LRAAGAPGTPAGRS PRRPGAAAVLGPEPGVVDRARAIPAVCCRSLAL QAP*GRRASGPASPSSPGQREPPGRARVDPASP GSRRAPLHPGRAQAQGLLGLSGC*PPVLERH*ED WSGVWCQPIPAAFIDSIQHCERNSLHCLGPALC DHQL*DIQGCDPSYPEIR*RPPIQGISGI*SCY I*GVGSEVQ*FCSWSCELHDKGTQAPLLS**FS* FSEVCSVDVGIYLCWCLV*WSDTTDFGSHFTLQ CSCYL*TASGTDRSLSRTCK*EC*RCYG*NPSK NPWIEAQS*MKTPKIISRSSSLKGIFI*LYGGGS GKNEP*RCSAVSQIVVRSLFLAMHCCEEKLPVL TAMCSSS*VL*AAMYGFKP*SYLFPIYLRHWWN KKPVYFTLLQIVLPHLGKLQRWWS*KKKKKSPFQ FVHCVWSV*IDADFLK*NVCLDEIIPVKQE*QS LLFWYVLGVL*LLLLY*LPI*VNIDYICIVFHK A*TFTFQPPHSA*YFRVSHWLYMCSSKAHKLEEE IFLGALPSVFNMKCHTHRTPTSISLHRLTVVNF VTESMD*I*CFQKCCLFANIKHCYARNY*LQNE DLYHCGLSCTELNLWNAL*TVKAKYQ*SL*T*NM L

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDEEEEEEEEEEDEDEDLEELEVLERKPAAGLS AAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAA PPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPS KLPEDDEPPARPPPPPPASVSPQAEPVWTPPAP APAAPPSTPAAPKRRGSSGSVVVDLLYWRDIKK TGVVFGASLFLLLSLTVFSIVSVTAYIALALLSV TISFRIYKGVIQAIQKSDEGHPFRAYLESEVAI SEELVQKYSNSALGHVNCTIKELRRLFLVDDLV DSLKFAVLMWVFTYVGALFNGLTLLILALISLFS VPVIYERHQAQIDHYLGLANKNVKDAMAKIQAK IPGLKRKAE*KRPK*LVGVHL*RGYSFDYTGEG QGRTNLDVAVQFHRSLLDLYF*PCTVVRKNYLS* LPCVHHLKYCKLLCMDLNRNHIFFLSI*GTGGI KNLYILLCCR*SCRILASCRDGGARKKKKKALF SLCTVYGPCRLMQIF*NEMFV*TRSYR*SRNDKA CFSGMF*VYCDFYCYINCQYK*I*IIYV*CFTK LRPLPSSHPTVLDISESVIGYTCVVPKHIS*KK KYF*EHYHLFST*NATHIELQHQFHCTD*L*LIL SQNLWTESNASKNVVCLQISNIVMQEIINYKMK IYTIVV*AVLN*ICGMHCEL*KQSINKAYRLKI C

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTRRKKRRRKRRTRTKTWRSWRCWRGSPPPGCP RPQCPPPLPPARP*WTSEMTSCRRRPGDPCRPL PPSPRSGSRLGTRARCRRPCPRHPRCLLPQSRP PSSLRTTSLRPGLPLLPRPA*APRQSPCGPRQPR LPPRPPPPRPRPSAGAPRAQWLLTSCTGETLRR LEWCLVPAYSCCFH*QYSAL*A*QPTLPWPCSL *PSALGYTRV*SKLSRNQMKATHSGHIWNLKLLY LRSWFRSTVILLLVM*TAR*RNSGASS*LMI*L IL*SLQC*CGYLPMLVPCLMV*HY*FWLSFHSS VFLLFMNGIRHR*III*DLQIRMLKMLWLKSKQK SLD*SAKLNENAQNN**EFIFKGDIHLIIRGRV REERTLTLQCSFTDRC*IFIFSHALL*GKITCL DCHVFIILSIVSCYVWI*TVIISFSYLSEALVE* KTCIFYFVADSLAASWQVAEMVELEKKKKKPFS VCALCMVRVD*CRFSEMKCLFRRDHTGKAGMTK LAFLVCSRCIVTFTVILIANISKYRLYMYSVSQS LDLYLPATPQCLIFQSQSLVIHV*FQST*ARRR NISRSTTICFQHEMPHT*NSNINFIAQTDCS*F CHRIYGLNLMLPKMLFVCKYQTLLCKKLLITK*R FIPLWFKLY*TKSVECIVNCKSKVSIKLIDLKY A

Sequence RTN4.kAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACG CTCCTCTGCAGAAAATATGGACTTGAAGGAGCAGCCAGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTGCTTGAAACTGCTGCTTCT CTTCCTTCTCTGTCTCCTCTCTCAGCCGCTTCTTTCAAAGAACATGAATACCTTGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGG TGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAG CTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAG TTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGA AGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGT TATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGA TTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAA GCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGA ATGACAAAGCTTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTG TTTCACAAAGCTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGC TAGAAGAAGAAATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGT AGTTAATTTTGTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTA CAAAATGAAGATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGA CTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Multiple exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSG*DPFCSSCCI*ACDT LLCRKYGLEGAAR*HYFGWSRGFPICPA*NCCF SSFSVSSLSRFFQRT*IPCC*PPVLERH*EDWSG VWCQPIPAAFIDSIQHCERNSLHCLGPALCDHQ L*DIQGCDPSYPEIR*RPPIQGISGI*SCYI*G VGSEVQ*FCSWSCELHDKGTQAPLLS**FS*FSE VCSVDVGIYLCWCLV*WSDTTDFGSHFTLQCSC YL*TASGTDRSLSRTCK*EC*RCYG*NPSKNPW IEAQS*MKTPKIINSLAASWQVAEMVELEKKKKK PFSVCALCMVRVD*CRFSEMKCLFRRDHTGKAG MTKLAFLVCSRCIVTFTVILIANISKYRLYMYS VSQSLDLYLPATPQCLIFQSQSLVIHV*FQST*A RRRNISRSTTICFQHEMPHT*NSNINFIAQTDC S*FCHRIYGLNLMLPKMLFVCKYQTLLCKKLLI TK*RFIPLWFKLY*TKSVECIVNCKSKVSIKLID LKYA

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVDETLFALPAASEPVIR SSAENMDLKEQPGNTISAGQEDFPSVLLETAAS LPSLSPLSAASFKEHEYLVVDLLYWRDIKKTGV VFGASLFLLLSLTVFSIVSVTAYIALALLSVTIS FRIYKGVIQAIQKSDEGHPFRAYLESEVAISEE LVQKYSNSALGHVNCTIKELRRLFLVDDLVDSL KFAVLMWVFTYVGALFNGLTLLILALISLFSVPV IYERHQAQIDHYLGLANKNVKDAMAKIQAKIPG LKRKAE*KRPK*LIVLPHLGKLQRWWS*KKKKK SPFQFVHCVWSV*IDADFLK*NVCLDEIIPVKQE *QSLLFWYVLGVL*LLLLY*LPI*VNIDYICIV FHKA*TFTFQPPHSA*YFRVSHWLYMCSSKAHK LEEEIFLGALPSVFNMKCHTHRTPTSISLHRLTV VNFVTESMD*I*CFQKCCLFANIKHCYARNY*L QNEDLYHCGLSCTELNLWNAL*TVKAKYQ*SL* T*NML

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWMRPFLLFLLHLSL*Y APLQKIWT*RSSQVTLFRLVKRISHLSCLKLLLL FLLCLLSQPLLSKNMNTLLLTSCTGETLRRLEW CLVPAYSCCFH*QYSAL*A*QPTLPWPCSL*PS ALGYTRV*SKLSRNQMKATHSGHIWNLKLLYLRS WFRSTVILLLVM*TAR*RNSGASS*LMI*LIL* SLQC*CGYLPMLVPCLMV*HY*FWLSFHSSVFL LFMNGIRHR*III*DLQIRMLKMLWLKSKQKSLD *SAKLNENAQNN**SCRILASCRDGGARKKKKK ALFSLCTVYGPCRLMQIF*NEMFV*TRSYR*SR NDKACFSGMF*VYCDFYCYINCQYK*I*IIYV*C FTKLRPLPSSHPTVLDISESVIGYTCVVPKHIS *KKKYF*EHYHLFST*NATHIELQHQFHCTD*L *LILSQNLWTESNASKNVVCLQISNIVMQEIINY KMKIYTIVV*AVLN*ICGMHCEL*KQSINKAYR LKIC