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Sequences

Sequence RTN4.iAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGT GTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGC TTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGT TGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAA GTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTT ATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGAT TGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTCAGGGAAGAAC GAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGATCTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTGACTGCCATGT GTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATAAAAAACCTGT ATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCAC TGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGCTTGCTTTTCT GGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAGCTTAGACCTT TACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGAAATATTTCTA GGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAAT CTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAGATTTATACCA TTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Single exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSGC*PPVLERH*EDWSG VWCQPIPAAFIDSIQHCERNSLHCLGPALCDHQ L*DIQGCDPSYPEIR*RPPIQGISGI*SCYI*GV GSEVQ*FCSWSCELHDKGTQAPLLS**FS*FSE VCSVDVGIYLCWCLV*WSDTTDFGSHFTLQCSC YL*TASGTDRSLSRTCK*EC*RCYG*NPSKNPWI EAQS*MKTPKIISRSSSLKGIFI*LYGGGSGKN EP*RCSAVSQIVVRSLFLAMHCCEEKLPVLTAM CSSS*VL*AAMYGFKP*SYLFPIYLRHWWNKKPV YFTLLQIVLPHLGKLQRWWS*KKKKKSPFQFVH CVWSV*IDADFLK*NVCLDEIIPVKQE*QSLLF WYVLGVL*LLLLY*LPI*VNIDYICIVFHKA*TF TFQPPHSA*YFRVSHWLYMCSSKAHKLEEEIFL GALPSVFNMKCHTHRTPTSISLHRLTVVNFVTE SMD*I*CFQKCCLFANIKHCYARNY*LQNEDLYH CGLSCTELNLWNAL*TVKAKYQ*SL*T*NML

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVVVDLLYWRDIKKTGVV FGASLFLLLSLTVFSIVSVTAYIALALLSVTIS FRIYKGVIQAIQKSDEGHPFRAYLESEVAISEE LVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLK FAVLMWVFTYVGALFNGLTLLILALISLFSVPV IYERHQAQIDHYLGLANKNVKDAMAKIQAKIPG LKRKAE*KRPK*LVGVHL*RGYSFDYTGEGQGRT NLDVAVQFHRSLLDLYF*PCTVVRKNYLS*LPC VHHLKYCKLLCMDLNRNHIFFLSI*GTGGIKNL YILLCCR*SCRILASCRDGGARKKKKKALFSLCT VYGPCRLMQIF*NEMFV*TRSYR*SRNDKACFS GMF*VYCDFYCYINCQYK*I*IIYV*CFTKLRP LPSSHPTVLDISESVIGYTCVVPKHIS*KKKYF* EHYHLFST*NATHIELQHQFHCTD*L*LILSQN LWTESNASKNVVCLQISNIVMQEIINYKMKIYT IVV*AVLN*ICGMHCEL*KQSINKAYRLKIC

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWLLTSCTGETLRRLEW CLVPAYSCCFH*QYSAL*A*QPTLPWPCSL*PSA LGYTRV*SKLSRNQMKATHSGHIWNLKLLYLRS WFRSTVILLLVM*TAR*RNSGASS*LMI*LIL* SLQC*CGYLPMLVPCLMV*HY*FWLSFHSSVFLL FMNGIRHR*III*DLQIRMLKMLWLKSKQKSLD *SAKLNENAQNN**EFIFKGDIHLIIRGRVREE RTLTLQCSFTDRC*IFIFSHALL*GKITCLDCHV FIILSIVSCYVWI*TVIISFSYLSEALVE*KTC IFYFVADSLAASWQVAEMVELEKKKKKPFSVCA LCMVRVD*CRFSEMKCLFRRDHTGKAGMTKLAFL VCSRCIVTFTVILIANISKYRLYMYSVSQSLDL YLPATPQCLIFQSQSLVIHV*FQST*ARRRNIS RSTTICFQHEMPHT*NSNINFIAQTDCS*FCHRI YGLNLMLPKMLFVCKYQTLLCKKLLITK*RFIP LWFKLY*TKSVECIVNCKSKVSIKLIDLKYA

Sequence RTN4.jAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACGAGGAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCC GCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTC CCCCCGTCGCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTC CAAGCTCCCTGAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCG GCTCCCGCCGCGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGA CTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGT GACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATA TCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTG ATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAG TGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAA ATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTC AGGGAAGAACGAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGATCTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTG ACTGCCATGTGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATA AAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCA GTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGC TTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATTGCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAG CTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGA AATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTT GTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAG ATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATAT GCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Single exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRRGGRRGGGRGGRGRRPGGAGGAGEEARRRAV RGPSAHRPCRRRAPDGLRK*LRAAGAPGTPAGRS PRRPGAAAVLGPEPGVVDRARAIPAVCCRSLAL QAP*GRRASGPASPSSPGQREPPGRARVDPASP GSRRAPLHPGRAQAQGLLGLSGC*PPVLERH*ED WSGVWCQPIPAAFIDSIQHCERNSLHCLGPALC DHQL*DIQGCDPSYPEIR*RPPIQGISGI*SCY I*GVGSEVQ*FCSWSCELHDKGTQAPLLS**FS* FSEVCSVDVGIYLCWCLV*WSDTTDFGSHFTLQ CSCYL*TASGTDRSLSRTCK*EC*RCYG*NPSK NPWIEAQS*MKTPKIISRSSSLKGIFI*LYGGGS GKNEP*RCSAVSQIVVRSLFLAMHCCEEKLPVL TAMCSSS*VL*AAMYGFKP*SYLFPIYLRHWWN KKPVYFTLLQIVLPHLGKLQRWWS*KKKKKSPFQ FVHCVWSV*IDADFLK*NVCLDEIIPVKQE*QS LLFWYVLGVL*LLLLY*LPI*VNIDYICIVFHK A*TFTFQPPHSA*YFRVSHWLYMCSSKAHKLEEE IFLGALPSVFNMKCHTHRTPTSISLHRLTVVNF VTESMD*I*CFQKCCLFANIKHCYARNY*LQNE DLYHCGLSCTELNLWNAL*TVKAKYQ*SL*T*NM L

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDEEEEEEEEEEDEDEDLEELEVLERKPAAGLS AAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAA PPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPS KLPEDDEPPARPPPPPPASVSPQAEPVWTPPAP APAAPPSTPAAPKRRGSSGSVVVDLLYWRDIKK TGVVFGASLFLLLSLTVFSIVSVTAYIALALLSV TISFRIYKGVIQAIQKSDEGHPFRAYLESEVAI SEELVQKYSNSALGHVNCTIKELRRLFLVDDLV DSLKFAVLMWVFTYVGALFNGLTLLILALISLFS VPVIYERHQAQIDHYLGLANKNVKDAMAKIQAK IPGLKRKAE*KRPK*LVGVHL*RGYSFDYTGEG QGRTNLDVAVQFHRSLLDLYF*PCTVVRKNYLS* LPCVHHLKYCKLLCMDLNRNHIFFLSI*GTGGI KNLYILLCCR*SCRILASCRDGGARKKKKKALF SLCTVYGPCRLMQIF*NEMFV*TRSYR*SRNDKA CFSGMF*VYCDFYCYINCQYK*I*IIYV*CFTK LRPLPSSHPTVLDISESVIGYTCVVPKHIS*KK KYF*EHYHLFST*NATHIELQHQFHCTD*L*LIL SQNLWTESNASKNVVCLQISNIVMQEIINYKMK IYTIVV*AVLN*ICGMHCEL*KQSINKAYRLKI C

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTRRKKRRRKRRTRTKTWRSWRCWRGSPPPGCP RPQCPPPLPPARP*WTSEMTSCRRRPGDPCRPL PPSPRSGSRLGTRARCRRPCPRHPRCLLPQSRP PSSLRTTSLRPGLPLLPRPA*APRQSPCGPRQPR LPPRPPPPRPRPSAGAPRAQWLLTSCTGETLRR LEWCLVPAYSCCFH*QYSAL*A*QPTLPWPCSL *PSALGYTRV*SKLSRNQMKATHSGHIWNLKLLY LRSWFRSTVILLLVM*TAR*RNSGASS*LMI*L IL*SLQC*CGYLPMLVPCLMV*HY*FWLSFHSS VFLLFMNGIRHR*III*DLQIRMLKMLWLKSKQK SLD*SAKLNENAQNN**EFIFKGDIHLIIRGRV REERTLTLQCSFTDRC*IFIFSHALL*GKITCL DCHVFIILSIVSCYVWI*TVIISFSYLSEALVE* KTCIFYFVADSLAASWQVAEMVELEKKKKKPFS VCALCMVRVD*CRFSEMKCLFRRDHTGKAGMTK LAFLVCSRCIVTFTVILIANISKYRLYMYSVSQS LDLYLPATPQCLIFQSQSLVIHV*FQST*ARRR NISRSTTICFQHEMPHT*NSNINFIAQTDCS*F CHRIYGLNLMLPKMLFVCKYQTLLCKKLLITK*R FIPLWFKLY*TKSVECIVNCKSKVSIKLIDLKY A

Sequence RTN4.hAug05

AGTCCCTGCCCTCCCCTGGGGAGGGTGAGTCACGCCAAACTGGGCGGAGAGTCCGCTGGCCTCACTCCTAGCTCATCTGGGCGGCGGCGGCAAGTGGGGA CAGGGCGGGTGGCGCATCACCGGCGCGGAGGCAGGAGGAGCAGTCTCATTGTTCCGGGAGCCGTCACCACAGTAGGTCCCTCGGCTCAGTCGGCCCAGCC CCTCTCAGTCCTCCCCAACCCCCACAACCGCCCGCGGCTCTGAGACGCGGCCCCGGCGGCGGCGGCAGCAGCTGCAGCATCATCTCCACCCTCCAGCCAT GGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAG GAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAG TGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTCGC CCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCT GAGGACGACGAGCCTCCGGCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCCCGGCTCCCGCCG CGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACG CTCCTCTGCAGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTC AGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAG ATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTG CACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTG TTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGGAC TTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGG AGTTCATCTTTAAAGGGGATATTCATTTGATTATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGTGCAGTTTCACAGATCGTTGTTAGAT CTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTGACTGCCATGTGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAA ACCGTAATCATATCTTTTTCCTATCTATCTGAGGCACTGGTGGAATAAAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG TTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAA ATGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGCTTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAATT GCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAGCTTAGACCTTTACCTTCCAGCCACCCCACAGTGCTTGATATTTCAGAGTCAGT CATTGGTTATACATGTGTAGTTCCAAAGCACATAAGCTAGAAGAAGAAATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATA GAACTCCAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAATCTATGGACTGAATCTAATGCTTCCAAAAATGTTGTTTGTTTGC AAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATGAAGATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTG TGAACTGTAAAAGCAAAGTATCAATAAAGCTTATAGACTTAAAATATGCTT

RTN4.u.f.6 (forward primer)
RTN4.au.5.r (reverse primer)
Single exon skipping

Frame 0

SPCPPLGRVSHAKLGGESAGLTPSSSGRRRQVG TGRVAHHRRGGRRSSLIVPGAVTTVGPSAQSAQP LSVLPNPHNRPRL*DAAPAAAAAAAASSPPSSH GRPGPVSSGLVLGQPTPAAARVQVPVREGARGR GGRRGGGRGGRGRRPGGAGGAGEEARRRAVRGPS AHRPCRRRAPDGLRK*LRAAGAPGTPAGRSPRR PGAAAVLGPEPGVVDRARAIPAVCCRSLALQAP *GRRASGPASPSSPGQREPPGRARVDPASPGSRR APLHPGRAQAQGLLGLSG*DPFCSSCCI*ACDT LLCSC*PPVLERH*EDWSGVWCQPIPAAFIDSI QHCERNSLHCLGPALCDHQL*DIQGCDPSYPEIR *RPPIQGISGI*SCYI*GVGSEVQ*FCSWSCEL HDKGTQAPLLS**FS*FSEVCSVDVGIYLCWCL V*WSDTTDFGSHFTLQCSCYL*TASGTDRSLSRT CK*EC*RCYG*NPSKNPWIEAQS*MKTPKIISR SSSLKGIFI*LYGGGSGKNEP*RCSAVSQIVVR SLFLAMHCCEEKLPVLTAMCSSS*VL*AAMYGFK P*SYLFPIYLRHWWNKKPVYFTLLQIVLPHLGK LQRWWS*KKKKKSPFQFVHCVWSV*IDADFLK* NVCLDEIIPVKQE*QSLLFWYVLGVL*LLLLY*L PI*VNIDYICIVFHKA*TFTFQPPHSA*YFRVS HWLYMCSSKAHKLEEEIFLGALPSVFNMKCHTH RTPTSISLHRLTVVNFVTESMD*I*CFQKCCLFA NIKHCYARNY*LQNEDLYHCGLSCTELNLWNAL *TVKAKYQ*SL*T*NML

Frame 1

VPALPWGG*VTPNWAESPLASLLAHLGGGGKWG QGGWRITGAEAGGAVSLFREPSPQ*VPRLSRPS PSQSSPTPTTARGSETRPRRRRQQLQHHLHPPAM EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDE EEEEEEEEEDEDEDLEELEVLERKPAAGLSAAP VPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVA PERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLP EDDEPPARPPPPPPASVSPQAEPVWTPPAPAPA APPSTPAAPKRRGSSGSVDETLFALPAASEPVIR SSAVVDLLYWRDIKKTGVVFGASLFLLLSLTVF SIVSVTAYIALALLSVTISFRIYKGVIQAIQKS DEGHPFRAYLESEVAISEELVQKYSNSALGHVNC TIKELRRLFLVDDLVDSLKFAVLMWVFTYVGAL FNGLTLLILALISLFSVPVIYERHQAQIDHYLG LANKNVKDAMAKIQAKIPGLKRKAE*KRPK*LVG VHL*RGYSFDYTGEGQGRTNLDVAVQFHRSLLD LYF*PCTVVRKNYLS*LPCVHHLKYCKLLCMDL NRNHIFFLSI*GTGGIKNLYILLCCR*SCRILAS CRDGGARKKKKKALFSLCTVYGPCRLMQIF*NE MFV*TRSYR*SRNDKACFSGMF*VYCDFYCYIN CQYK*I*IIYV*CFTKLRPLPSSHPTVLDISESV IGYTCVVPKHIS*KKKYF*EHYHLFST*NATHI ELQHQFHCTD*L*LILSQNLWTESNASKNVVCL QISNIVMQEIINYKMKIYTIVV*AVLN*ICGMHC EL*KQSINKAYRLKIC

Frame 2

SLPSPGEGESRQTGRRVRWPHS*LIWAAAASGD RAGGASPARRQEEQSHCSGSRHHSRSLGSVGPA PLSPPQPPQPPAALRRGPGGGGSSCSIISTLQP WKTWTSLLWSRPRTAHPGRSPRSSTSS*GSPRTR RKKRRRKRRTRTKTWRSWRCWRGSPPPGCPRPQ CPPPLPPARP*WTSEMTSCRRRPGDPCRPLPPS PRSGSRLGTRARCRRPCPRHPRCLLPQSRPPSSL RTTSLRPGLPLLPRPA*APRQSPCGPRQPRLPP RPPPPRPRPSAGAPRAQWMRPFLLFLLHLSL*Y APLQLLTSCTGETLRRLEWCLVPAYSCCFH*QYS AL*A*QPTLPWPCSL*PSALGYTRV*SKLSRNQ MKATHSGHIWNLKLLYLRSWFRSTVILLLVM*T AR*RNSGASS*LMI*LIL*SLQC*CGYLPMLVPC LMV*HY*FWLSFHSSVFLLFMNGIRHR*III*D LQIRMLKMLWLKSKQKSLD*SAKLNENAQNN** EFIFKGDIHLIIRGRVREERTLTLQCSFTDRC*I FIFSHALL*GKITCLDCHVFIILSIVSCYVWI* TVIISFSYLSEALVE*KTCIFYFVADSLAASWQ VAEMVELEKKKKKPFSVCALCMVRVD*CRFSEMK CLFRRDHTGKAGMTKLAFLVCSRCIVTFTVILI ANISKYRLYMYSVSQSLDLYLPATPQCLIFQSQ SLVIHV*FQST*ARRRNISRSTTICFQHEMPHT* NSNINFIAQTDCS*FCHRIYGLNLMLPKMLFVC KYQTLLCKKLLITK*RFIPLWFKLY*TKSVECI VNCKSKVSIKLIDLKYA