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Sequences

Sequence PTGS1.eAug05

AGGTGACAGCTGGAGGGAGGAGCGGGGGTGGAGCCGGGGGAAGGGTGGGGAGGGGATGGGCTGGAGCTCCGGGCAGTGTGCGAGGCGCACGCACAGGAGC CTGCACTCTGCGTCCCGCACCCCAGCAGCCGCGCCATGAGCCGGAGTCTCTTGCTCTGGTTCTTGCTGTTCCTGCTCCTGCTCCCGCCGCTCCCCGTCCT GCTCGCGGACCCAGGGGCGCCCACGCCAGCACCAGGGCATCTGTGTCCGCTTCGGCCTTGACCGCTACCAGTGTGACTGCACCCGCACGGGCTATTCCGG CCCCAACTGCACCATCCCTGGCCTGTGGACCTGGCTCCGGAATTCACTGCGGCCCAGCCCCTCTTTCACCCACTTCCTGCTCACTCACGGGCGCTGGTTC TGGGAGTTTGTCAATGCCACCTTCATCCGAGAGATGCTCATGCGCCTGGTACTCACAGTGCGCTCCAACCTTATCCCCAGTCCCCCCACCTACAACTCAG CACATGACTACATCAGCTGGGAGTCTTTCTCCAACGTGAGCTATTACACTCGTATTCTGCCCTCTGTGCCTAAAGATTGCCCCACACCCATGGGAACCAA AGGGAAGAAGCAGTTGCCAGATGCCCAGCTCCTGGCCCGCCGCTTCCTGCTCAGGAGGAAGTTCATACCTGACCCCCAAGGCACCAACCTCATGTTTGCC TTCTTTGCACAACACTTCACCCACCAGTTCTTCAAAACTTCTGGCAAGATGGGTCCTGGCTTCACCAAGGCCTTGGGCCATGGGGTAGACCTCGGCCACA TTTATGGAGACAATCTGGAGCGTCAGTATCAACTGCGGCTCTTTAAGGATGGGAAACTCAAGTACCAGGTGCTGGATGGAGAAATGTACCCGCCCTCGGT AGAAGAGGCGCCTGTGTTGATGCACTACCCCCGAGGCATCCCGCCCCAGAGCCAGATGGCTGTGGGCCAGGAGGTGTTTGGGCTGCTTCCTGGGCTCATG CTGTATGCCACGCTCTGGCTACGTGAGCACAACCGTGTGTGTGACCTGCTGAAGGCTGAGCACCCCACCTGGGGCGATGAGCAGCTTTTCCAGACGACCC GCCTCATCCTCATAGGGGAGACCATCAAGATTGTCATCGAGGAGTACGTGCAGCAGCTGAGTGGCTATTTCCTGCAGCTGAAATTTGACCCAGAGCTGCT GTTCGGTGTCCAGTTCCAATACCGCAACCGCATTGCCATGGAGTTCAACCATCTCTACCACTGGCACCCCCTCATGCCTGACTCCTTCAAGATCGGTGGG GGCAGGAACATGGACCACCACATCCTGCATGTGGCTGTGGATGTCATCAGGGAGTCTCGGGAGATGCGGCTGCAGCCCTTCAATGAGTACCGCAAGAGGT TTGGCATGAAACCCTACACCTCCTTCCAGGAGCTCGTAGGAGAGAAGGAGATGGCAGCAGAGTTGGAGGAATTGTATGGAGACATTGATGCGTTGGAGTT CTACCCTGGACTGCTTCTTGAAAAGTGCCATCCAAACTCTATCTTTGGGGAGAGTATGATAGAGATTGGGGCTCCCTTTTCCCTCAAGGGTCTCCTAGGG AATCCCATCTGTTCTCCGGAGTACTGGAAGCCGAGCACATTTGGCGGCGAGGTGGGCTTTAACATTGTCAAGACGGCCACACTGAAGAAGCTGGTCTGCC TCAACACCAAGACCTGTCCCTACGTTTCCTTCCGTGTGCCGGATGCCAGTCAGGATGATGGGCCTGCTGTGGAGCGACCATCCACAGAGCTCTGAGGGGC AGGAAAGCAGCATTCTGGAGGGGAGAGCTTTGTGCTTGTCATTCCAGAGTGCTGAGGCCAGGGCTGATGGTCTTAAATGCTCATTTTCTGGTTTGGCATG GTGAGTGTTGGGGTTGACATTTAGAACTTTAAGTCTCACCCATTATCTGGAATATTGTGATTCTGTTTATTCTTCCAGAATGCTGAACTCCTTGTTAGCC CTTCAGATTGTTAGGAGTGGTTCTCATTTGGTCTGCCAGAATACTGGGTTCTTAGTTGACAACCTAGAATGTCAGATTTCTGGTTGATTTGTAACACAGT CATTCTAGGATGTGGAGCTACTGATGAAATCTGCTAGAAAGTTAGGGGGTTCTTATTTTGCATTCCAGAATCTTGACTTTCTGATTGGTGATTCAAAGTG TTGTGTTCCTGGCTGATGATCCAGAACAGTGGCTCGTATCCCAAATCTGTCAGCATCTGGCTGTCTAGAATGTGGATTTGATTCATTTTCCTGTTCAGTG AGATATCATAGAGACGGAGATCCTAAGGTCCAACAAGAATGCATTCCCTGAATCTGTGCCTGCACTGAGAGGGCAAGGAAGTGGGGTGTTCTTCTTGGGA CCCCCACTAAGACCCTGGTCTGAGGATGTAGAGAGAACAGGTGGGCTGTATTCACGCCATTGGTTGGAAGCTACCAGAGCTCTATCCCCATCCAGGTCTT GACTCATGGCAGCTGTTTCTCATGAAGCTAATAAAATTCGCTTTCTAAAGTTACCTGTTATATATCTCTTTTGGTCCCATCCTCTAAAGCAGAGGCAACA CTGGAACATGGCTAGCCTTTCTTGTAGCCATGGCTGGGCGTGCTAGAGGTTGCAGCATGAGACTTTCTGCTGGGATCCTTGGGCCCATCACTGTATAGAC ATGCTACCACTGGTACTTCCTTTCTCCCTGCGGGCCAGGCACTGCCCTTTTCAGGAAGCTCTCTTAAAATACCCATTGCCCCAGACCTGGAAGATATAAC ATTCAGTTCCCACCATCTGATTAAAACAACTTCCTCCCTTACAGAGCATACAACAGAGGGGGCACCCGGGGAGGAGAGCACATACTGTGTTCCAATTTCA CGCTTTTAATTCTCATTTGTTCTCACACCAACAGTGTGAAGTGCGTGGTATAATCTCCATTTCAAAACCAAGGAAGCAGCCTCAGAGTGGTCGAGTGACA CACCTCACGCAGGCTGAGTCCAGAGCTTGTGCTCCTCTTGATTCCTGGTTTGACTCAGTTCCAGGCCTGATCTTGCCTGTCTGGCTCAGGGTCAAAGACA GAATGGTGGAGTGTAGCCTCCACCTGATATTCAGGCTACTCATTCAGTCCCAAATATGTATTTTCCTAAGTGTTTACTATGTGCCAGTTCCTGTAACAGG TGTGGGGACACAGCAGTGAGTAATCAATACAGACAAGGTTCTGCCCTTATGGAGCTCACACTCCAGTGGCAGACAAACAGACCATAAATAAGGAAACGAT GAAATAAGATATATACAAGGTGAGTGTGACTTCCCTTCTAACCCCCTCTGCTCTGTCCTCCCCTATTGCGCTCTCAAGACCAGAGACCCAACAGCAGTGA TCTCAGGGCAGACAGCCCTCCACTCCAGCTCTGAGACCCTTTTCTCAGGACCTCTGTAGGCAGCAGAGAGAGAGGACAGAGGGGTAAGATGAGGGGTTGA GGGAAGGTTCTTCATGATCCACACTTTGGGCTTAGTATTTCTCAGGAAGAGCTATGGCCCAGAAACAACAGGGGAAACTAGAGTTCGGTCTGACAGTCCT TGGGGTTAAGTCTCCTGTCTTATGGTCCAGAAACTCCTGTTTCTCCTTAGTTGGCTGGAAACTGCTCCCATCATTCCTTCTGGCCTCTGCTGAATGCAGG GAATGCAATCCTTCCCTGCTCTTGCAGTTGCTCTGACGTAGAAAGATCCTTCGGGTGCTGGAAGTCTCCATGAAGAGCTTGTGTCCTGTCCTTTCTTGCA GATTCTATTTCCCCTCTTCTGCTAATACCTCTTACTTTGCTTGAGAATCCTCTCCTTTCTTATTAATTTCAGTCTTGGTGGTTCTATCAGGGGTGCATTC TGGCCAAGGGGTGGGCCTGTGAATCAATCCTGGGCAATCAGACACCCTCTCCTTAAAAACTGGCCCGTGGAGACTGAGATCACTGACTCTGACTCATCCC CACAGCTGGCTCTGACAAGATGGTCCATTTGTTCCTGCTTCCGAGATCCCCAGGGCAGCCTGGATCCCTGCCCTTCTCAAGACTTTAGCTTTTCCTTCCA TCCGGTGGCCTATTCCAGGAATTCCTCTTTTGCTTAAATCAGTTGGAGTTTGTGTCTGTTGCTTGTAATCAAGCCTTTATGGCTGCTGGGCTGAGTGACA CAAGCACTTTAATGGCCTGGAGGGACTTTTAATCAGTGAAGATGCAATCAGACAAGTGTTTTGGAAAGAGCACCCTCGAGAAGGGTGGATGACAGGGCAG AGCAGGAAGGACAGGAAGCTGGCAGAACGGAGGAGGCTGCAGCCGTGGTCCAACCAGGAGCTGATGGCAGCTGGGGCTAGGGGAAGGGCTTTGAGGGTGG AAGGATGGGATGGGTTCCAGAGGTATTCCTCTCTTAAATGCAAGTGCCTAGATTAGGTAGACTTTGCTTAGTATTGACAACTGCACATGAAAGTTTTGCA AAGGGAAACAGGCTAAATGCACCAAGAAAGCTTCTTCAGAGTGAAGAATCTTAATGCTTGTAATTTAAACATTTGTTCCTGGAGTTTTGATTTGGTGGAT GTGATGGTTGGTTTTATTTGTCAGTTTGGTTGGGCTATAGCACACAGTTATTTAATCAAACAGTAATCTAGGTGTGGCTGTGAAGGTATTTTGTAGATGT GATTAACATCTACAATCAGTTGACTTTAAGTGAAAGAGATTACTTAAATAATTTGGGTGAGCTGCACCTGATTAGTTGAAAGGCCTCAAGAACAAACACT GCAGTTTCCTGGAAAAGAAGAAACTTTGCCTCAAGACTATAGCCATCGACTCCTGCCTGAGTTTCCAGCCTGCTAGTCTGCCCTATGGATTTGAAGTTTG CCAACCCCAACAATTGTGTGAATTAATTTCTAAAAATAAAGCTATATACAGCCATCCTTTGGTATTTGTGGGGGATTTGTTTCAGGATCTCTACAGATAC CAAAATCTGTGGTTCCTCAAGTCCTTTATATGAAGTGGTATAGTATTTGCATATAACCTATGTGCAATCTCCTGTATAACTTCAATCATCTCTAGATTAC TTATAATACCTAATACAGTGTAAATGCTAGTTGTTATACTGT

PTGS1.u.f.15 (forward primer)
PTGS1.u.r.17 (reverse primer)
Alternative donor

Frame 0

R*QLEGGAGVEPGEGWGGDGLELRAVCEAHAQE PALCVPHPSSRAMSRSLLLWFLLFLLLLPPLPVL LADPGAPTPAPGHLCPLRP*PLPV*LHPHGLFR PQLHHPWPVDLAPEFTAAQPLFHPLPAHSRALV LGVCQCHLHPRDAHAPGTHSALQPYPQSPHLQLS T*LHQLGVFLQRELLHSYSALCA*RLPHTHGNQ REEAVARCPAPGPPLPAQEEVHT*PPRHQPHVC LLCTTLHPPVLQNFWQDGSWLHQGLGPWGRPRPH LWRQSGASVSTAAL*GWETQVPGAGWRNVPALG RRGACVDALPPRHPAPEPDGCGPGGVWAASWAH AVCHALAT*AQPCV*PAEG*APHLGR*AAFPDDP PHPHRGDHQDCHRGVRAAAEWLFPAAEI*PRAA VRCPVPIPQPHCHGVQPSLPLAPPHA*LLQDRW GQEHGPPHPACGCGCHQGVSGDAAAALQ*VPQEV WHETLHLLPGARRREGDGSRVGGIVWRH*CVGV LPWTAS*KVPSKLYLWGEYDRDWGSLFPQGSPR ESHLFSGVLEAEHIWRRGGL*HCQDGHTEEAGLP QHQDLSLRFLPCAGCQSG*WACCGATIHRALRG RKAAFWRGELCACHSRVLRPGLMVLNAHFLVWH GECWG*HLEL*VSPIIWNIVILFILPEC*TPC*P FRLLGVVLIWSARILGS*LTT*NVRFLVDL*HS HSRMWSY**NLLES*GVLILHSRILTF*LVIQS VVFLADDPEQWLVSQICQHLAV*NVDLIHFPVQ* DIIETEILRSNKNAFPESVPALRGQGSGVFFLG PPLRPWSEDVERTGGLYSRHWLEATRALSPSRS *LMAAVSHEANKIRFLKLPVIYLFWSHPLKQRQH WNMASLSCSHGWAC*RLQHETFCWDPWAHHCID MLPLVLPFSLRARHCPFQEALLKYPLPQTWKI* HSVPTI*LKQLPPLQSIQQRGHPGRRAHTVFQFH AFNSHLFSHQQCEVRGIISISKPRKQPQSGRVT HLTQAESRACAPLDSWFDSVPGLILPVWLRVKD RMVECSLHLIFRLLIQSQICIFLSVYYVPVPVTG VGTQQ*VINTDKVLPLWSSHSSGRQTDHK*GND EIRYIQGECDFPSNPLCSVLPYCALKTRDPTAV ISGQTALHSSSETLFSGPL*AAEREDRGVR*GVE GRFFMIHTLGLVFLRKSYGPETTGETRVRSDSP WG*VSCLMVQKLLFLLSWLETAPIIPSGLC*MQ GMQSFPALAVALT*KDPSGAGSLHEELVSCPFLQ ILFPLFC*YLLLCLRILSFLINFSLGGSIRGAF WPRGGPVNQSWAIRHPLLKNWPVETEITDSDSS PQLALTRWSICSCFRDPQGSLDPCPSQDFSFSFH PVAYSRNSSFA*ISWSLCLLLVIKPLWLLG*VT QAL*WPGGTFNQ*RCNQTSVLERAPSRRVDDRA EQEGQEAGRTEEAAAVVQPGADGSWG*GKGFEGG RMGWVPEVFLS*MQVPRLGRLCLVLTTAHESFA KGNRLNAPRKLLQSEES*CL*FKHLFLEF*FGG CDGWFYLSVWLGYSTQLFNQTVI*VWL*RYFVDV INIYNQLTLSERDYLNNLGELHLIS*KASRTNT AVSWKRRNFASRL*PSTPA*VSSLLVCPMDLKF ANPNNCVN*FLKIKLYTAILWYLWGICFRISTDT KICGSSSPLYEVV*YLHITYVQSPV*LQSSLDY L*YLIQCKC*LLYC

Frame 1

GDSWREERGWSRGKGGEGMGWSSGQCARRTHRS LHSASRTPAAAP*AGVSCSGSCCSCSCSRRSPS CSRTQGRPRQHQGICVRFGLDRYQCDCTRTGYSG PNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWF WEFVNATFIREMLMRLVLTVRSNLIPSPPTYNS AHDYISWESFSNVSYYTRILPSVPKDCPTPMGTK GKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFA FFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGH IYGDNLERQYQLRLFKDGKLKYQVLDGEMYPPSV EEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLM LYATLWLREHNRVCDLLKAEHPTWGDEQLFQTT RLILIGETIKIVIEEYVQQLSGYFLQLKFDPELL FGVQFQYRNRIAMEFNHLYHWHPLMPDSFKIGG GRNMDHHILHVAVDVIRESREMRLQPFNEYRKR FGMKPYTSFQELVGEKEMAAELEELYGDIDALEF YPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLG NPICSPEYWKPSTFGGEVGFNIVKTATLKKLVC LNTKTCPYVSFRVPDASQDDGPAVERPSTEL*GA GKQHSGGESFVLVIPEC*GQG*WS*MLIFWFGM VSVGVDI*NFKSHPLSGIL*FCLFFQNAELLVS PSDC*EWFSFGLPEYWVLS*QPRMSDFWLICNTV ILGCGATDEIC*KVRGFLFCIPES*LSDW*FKV LCSWLMIQNSGSYPKSVSIWLSRMWI*FIFLFS EIS*RRRS*GPTRMHSLNLCLH*EGKEVGCSSWD PH*DPGLRM*REQVGCIHAIGWKLPELYPHPGL DSWQLFLMKLIKFAF*SYLLYISFGPIL*SRGN TGTWLAFLVAMAGRARGCSMRLSAGILGPITV*T CYHWYFLSPCGPGTALFRKLS*NTHCPRPGRYN IQFPPSD*NNFLPYRAYNRGGTRGGEHILCSNF TLLILICSHTNSVKCVV*SPFQNQGSSLRVVE*H TSRRLSPELVLLLIPGLTQFQA*SCLSGSGSKT EWWSVAST*YSGYSFSPKYVFS*VFTMCQFL*Q VWGHSSE*SIQTRFCPYGAHTPVADKQTINKETM K*DIYKVSVTSLLTPSALSSPIALSRPETQQQ* SQGRQPSTPALRPFSQDLCRQQRERTEG*DEGL REGSS*STLWA*YFSGRAMAQKQQGKLEFGLTVL GVKSPVLWSRNSCFSLVGWKLLPSFLLASAECR ECNPSLLLQLL*RRKILRVLEVSMKSLCPVLSC RFYFPSSANTSYFA*ESSPFLLISVLVVLSGVHS GQGVGL*INPGQSDTLSLKTGPWRLRSLTLTHP HSWL*QDGPFVPASEIPRAAWIPALLKTLAFPS IRWPIPGIPLLLKSVGVCVCCL*SSLYGCWAE*H KHFNGLEGLLISEDAIRQVFWKEHPREGWMTGQ SRKDRKLAERRRLQPWSNQELMAAGARGRALRV EGWDGFQRYSSLKCKCLD*VDFA*Y*QLHMKVLQ RETG*MHQESFFRVKNLNACNLNICSWSFDLVD VMVGFICQFGWAIAHSYLIKQ*SRCGCEGIL*M *LTSTIS*L*VKEIT*IIWVSCT*LVERPQEQTL QFPGKEETLPQDYSHRLLPEFPAC*SALWI*SL PTPTIV*INF*K*SYIQPSFGICGGFVSGSLQI PKSVVPQVLYMKWYSICI*PMCNLLYNFNHL*IT YNT*YSVNASCYT

Frame 2

VTAGGRSGGGAGGRVGRGWAGAPGSVRGARTGA CTLRPAPQQPRHEPESLALVLAVPAPAPAAPRP ARGPRGAHASTRASVSASALTATSVTAPARAIP APTAPSLACGPGSGIHCGPAPLSPTSCSLTGAGS GSLSMPPSSERCSCAWYSQCAPTLSPVPPPTTQ HMTTSAGSLSPT*AITLVFCPLCLKIAPHPWEP KGRSSCQMPSSWPAASCSGGSSYLTPKAPTSCLP SLHNTSPTSSSKLLARWVLASPRPWAMG*TSAT FMETIWSVSINCGSLRMGNSSTRCWMEKCTRPR *KRRLC*CTTPEASRPRARWLWARRCLGCFLGSC CMPRSGYVSTTVCVTC*RLSTPPGAMSSFSRRP ASSS*GRPSRLSSRSTCSS*VAISCS*NLTQSC CSVSSSNTATALPWSSTISTTGTPSCLTPSRSVG AGTWTTTSCMWLWMSSGSLGRCGCSPSMSTARG LA*NPTPPSRSS*ERRRWQQSWRNCMETLMRWS STLDCFLKSAIQTLSLGRV**RLGLPFPSRVS*G IPSVLRSTGSRAHLAARWALTLSRRPH*RSWSA STPRPVPTFPSVCRMPVRMMGLLWSDHPQSSEG QESSILEGRALCLSFQSAEARADGLKCSFSGLAW *VLGLTFRTLSLTHYLEYCDSVYSSRMLNSLLA LQIVRSGSHLVCQNTGFLVDNLECQISG*FVTQ SF*DVELLMKSARKLGGSYFAFQNLDFLIGDSKC CVPG**SRTVARIPNLSASGCLECGFDSFSCSV RYHRDGDPKVQQECIP*ICACTERARKWGVLLG TPTKTLV*GCRENRWAVFTPLVGSYQSSIPIQVL THGSCFS*S**NSLSKVTCYISLLVPSSKAEAT LEHG*PFL*PWLGVLEVAA*DFLLGSLGPSLYR HATTGTSFLPAGQALPFSGSSLKIPIAPDLEDIT FSSHHLIKTTSSLTEHTTEGAPGEESTYCVPIS RF*FSFVLTPTV*SAWYNLHFKTKEAASEWSSD TPHAG*VQSLCSS*FLV*LSSRPDLACLAQGQRQ NGGV*PPPDIQATHSVPNMYFPKCLLCASSCNR CGDTAVSNQYRQGSALMELTLQWQTNRP*IRKR *NKIYTR*V*LPF*PPLLCPPLLRSQDQRPNSSD LRADSPPLQL*DPFLRTSVGSRERGQRGKMRG* GKVLHDPHFGLSISQEELWPRNNRGN*SSV*QS LGLSLLSYGPETPVSP*LAGNCSHHSFWPLLNAG NAILPCSCSCSDVERSFGCWKSP*RACVLSFLA DSISPLLLIPLTLLENPLLSY*FQSWWFYQGCI LAKGWACESILGNQTPSP*KLARGD*DH*L*LIP TAGSDKMVHLFLLPRSPGQPGSLPFSRL*LFLP SGGLFQEFLFCLNQLEFVSVACNQAFMAAGLSD TSTLMAWRDF*SVKMQSDKCFGKSTLEKGG*QGR AGRTGSWQNGGGCSRGPTRS*WQLGLGEGL*GW KDGMGSRGIPLLNASA*IR*TLLSIDNCT*KFC KGKQAKCTKKASSE*RILMLVI*TFVPGVLIWWM *WLVLFVSLVGL*HTVI*SNSNLGVAVKVFCRC D*HLQSVDFK*KRLLK*FG*AAPD*LKGLKNKH CSFLEKKKLCLKTIAIDSCLSFQPASLPYGFEVC QPQQLCELISKNKAIYSHPLVFVGDLFQDLYRY QNLWFLKSFI*SGIVFAYNLCAISCITSIISRL LIIPNTV*MLVVIL

Sequence PTGS1.bAug05

AGGTGACAGCTGGAGGGAGGAGCGGGGGTGGAGCCGGGGGAAGGGTGGGGAGGGGATGGGCTGGAGCTCCGGGCAGTGTGCGAGGCGCACGCACAGGAGC CTGCACTCTGCGTCCCGCACCCCAGCAGCCGCGCCATGAGCCGGAGTCTCTTGCTCTGGTTCTTGCTGTTCCTGCTCCTGCTCCCGCCGCTCCCCGTCCT GCTCGCGGACCCAGGGGCGCCCACGCCAGTGAATCCCTGTTGTTACTATCCATGCCAGCACCAGGGCATCTGTGTCCGCTTCGGCCTTGACCGCTACCAG TGTGACTGCACCCGCACGGGCTATTCCGGCCCCAACTGCACCATCCCTGGCCTGTGGACCTGGCTCCGGAATTCACTGCGGCCCAGCCCCTCTTTCACCC ACTTCCTGCTCACTCACGGGCGCTGGTTCTGGGAGTTTGTCAATGCCACCTTCATCCGAGAGATGCTCATGCGCCTGGTACTCACAGTGCGCTCCAACCT TATCCCCAGTCCCCCCACCTACAACTCAGCACATGACTACATCAGCTGGGAGTCTTTCTCCAACGTGAGCTATTACACTCGTATTCTGCCCTCTGTGCCT AAAGATTGCCCCACACCCATGGGAACCAAAGGGAAGAAGCAGTTGCCAGATGCCCAGCTCCTGGCCCGCCGCTTCCTGCTCAGGAGGAAGTTCATACCTG ACCCCCAAGGCACCAACCTCATGTTTGCCTTCTTTGCACAACACTTCACCCACCAGTTCTTCAAAACTTCTGGCAAGATGGGTCCTGGCTTCACCAAGGC CTTGGGCCATGGGGTAGACCTCGGCCACATTTATGGAGACAATCTGGAGCGTCAGTATCAACTGCGGCTCTTTAAGGATGGGAAACTCAAGTACCAGGTG CTGGATGGAGAAATGTACCCGCCCTCGGTAGAAGAGGCGCCTGTGTTGATGCACTACCCCCGAGGCATCCCGCCCCAGAGCCAGATGGCTGTGGGCCAGG AGGTGTTTGGGCTGCTTCCTGGGCTCATGCTGTATGCCACGCTCTGGCTACGTGAGCACAACCGTGTGTGTGACCTGCTGAAGGCTGAGCACCCCACCTG GGGCGATGAGCAGCTTTTCCAGACGACCCGCCTCATCCTCATAGGGGAGACCATCAAGATTGTCATCGAGGAGTACGTGCAGCAGCTGAGTGGCTATTTC CTGCAGCTGAAATTTGACCCAGAGCTGCTGTTCGGTGTCCAGTTCCAATACCGCAACCGCATTGCCATGGAGTTCAACCATCTCTACCACTGGCACCCCC TCATGCCTGACTCCTTCAAGATCGGTGGGGGCAGGAACATGGACCACCACATCCTGCATGTGGCTGTGGATGTCATCAGGGAGTCTCGGGAGATGCGGCT GCAGCCCTTCAATGAGTACCGCAAGAGGTTTGGCATGAAACCCTACACCTCCTTCCAGGAGCTCGTAGGAGAGAAGGAGATGGCAGCAGAGTTGGAGGAA TTGTATGGAGACATTGATGCGTTGGAGTTCTACCCTGGACTGCTTCTTGAAAAGTGCCATCCAAACTCTATCTTTGGGGAGAGTATGATAGAGATTGGGG CTCCCTTTTCCCTCAAGGGTCTCCTAGGGAATCCCATCTGTTCTCCGGAGTACTGGAAGCCGAGCACATTTGGCGGCGAGGTGGGCTTTAACATTGTCAA GACGGCCACACTGAAGAAGCTGGTCTGCCTCAACACCAAGACCTGTCCCTACGTTTCCTTCCGTGTGCCGGATGCCAGTCAGGATGATGGGCCTGCTGTG GAGCGACCATCCACAGAGCTCTGAGGGGCAGGAAAGCAGCATTCTGGAGGGGAGAGCTTTGTGCTTGTCATTCCAGAGTGCTGAGGCCAGGGCTGATGGT CTTAAATGCTCATTTTCTGGTTTGGCATGGTGAGTGTTGGGGTTGACATTTAGAACTTTAAGTCTCACCCATTATCTGGAATATTGTGATTCTGTTTATT CTTCCAGAATGCTGAACTCCTTGTTAGCCCTTCAGATTGTTAGGAGTGGTTCTCATTTGGTCTGCCAGAATACTGGGTTCTTAGTTGACAACCTAGAATG TCAGATTTCTGGTTGATTTGTAACACAGTCATTCTAGGATGTGGAGCTACTGATGAAATCTGCTAGAAAGTTAGGGGGTTCTTATTTTGCATTCCAGAAT CTTGACTTTCTGATTGGTGATTCAAAGTGTTGTGTTCCTGGCTGATGATCCAGAACAGTGGCTCGTATCCCAAATCTGTCAGCATCTGGCTGTCTAGAAT GTGGATTTGATTCATTTTCCTGTTCAGTGAGATATCATAGAGACGGAGATCCTAAGGTCCAACAAGAATGCATTCCCTGAATCTGTGCCTGCACTGAGAG GGCAAGGAAGTGGGGTGTTCTTCTTGGGACCCCCACTAAGACCCTGGTCTGAGGATGTAGAGAGAACAGGTGGGCTGTATTCACGCCATTGGTTGGAAGC TACCAGAGCTCTATCCCCATCCAGGTCTTGACTCATGGCAGCTGTTTCTCATGAAGCTAATAAAATTCGCTTTCTAAAGTTACCTGTTATATATCTCTTT TGGTCCCATCCTCTAAAGCAGAGGCAACACTGGAACATGGCTAGCCTTTCTTGTAGCCATGGCTGGGCGTGCTAGAGGTTGCAGCATGAGACTTTCTGCT GGGATCCTTGGGCCCATCACTGTATAGACATGCTACCACTGGTACTTCCTTTCTCCCTGCGGGCCAGGCACTGCCCTTTTCAGGAAGCTCTCTTAAAATA CCCATTGCCCCAGACCTGGAAGATATAACATTCAGTTCCCACCATCTGATTAAAACAACTTCCTCCCTTACAGAGCATACAACAGAGGGGGCACCCGGGG AGGAGAGCACATACTGTGTTCCAATTTCACGCTTTTAATTCTCATTTGTTCTCACACCAACAGTGTGAAGTGCGTGGTATAATCTCCATTTCAAAACCAA GGAAGCAGCCTCAGAGTGGTCGAGTGACACACCTCACGCAGGCTGAGTCCAGAGCTTGTGCTCCTCTTGATTCCTGGTTTGACTCAGTTCCAGGCCTGAT CTTGCCTGTCTGGCTCAGGGTCAAAGACAGAATGGTGGAGTGTAGCCTCCACCTGATATTCAGGCTACTCATTCAGTCCCAAATATGTATTTTCCTAAGT GTTTACTATGTGCCAGTTCCTGTAACAGGTGTGGGGACACAGCAGTGAGTAATCAATACAGACAAGGTTCTGCCCTTATGGAGCTCACACTCCAGTGGCA GACAAACAGACCATAAATAAGGAAACGATGAAATAAGATATATACAAGGTGAGTGTGACTTCCCTTCTAACCCCCTCTGCTCTGTCCTCCCCTATTGCGC TCTCAAGACCAGAGACCCAACAGCAGTGATCTCAGGGCAGACAGCCCTCCACTCCAGCTCTGAGACCCTTTTCTCAGGACCTCTGTAGGCAGCAGAGAGA GAGGACAGAGGGGTAAGATGAGGGGTTGAGGGAAGGTTCTTCATGATCCACACTTTGGGCTTAGTATTTCTCAGGAAGAGCTATGGCCCAGAAACAACAG GGGAAACTAGAGTTCGGTCTGACAGTCCTTGGGGTTAAGTCTCCTGTCTTATGGTCCAGAAACTCCTGTTTCTCCTTAGTTGGCTGGAAACTGCTCCCAT CATTCCTTCTGGCCTCTGCTGAATGCAGGGAATGCAATCCTTCCCTGCTCTTGCAGTTGCTCTGACGTAGAAAGATCCTTCGGGTGCTGGAAGTCTCCAT GAAGAGCTTGTGTCCTGTCCTTTCTTGCAGATTCTATTTCCCCTCTTCTGCTAATACCTCTTACTTTGCTTGAGAATCCTCTCCTTTCTTATTAATTTCA GTCTTGGTGGTTCTATCAGGGGTGCATTCTGGCCAAGGGGTGGGCCTGTGAATCAATCCTGGGCAATCAGACACCCTCTCCTTAAAAACTGGCCCGTGGA GACTGAGATCACTGACTCTGACTCATCCCCACAGCTGGCTCTGACAAGATGGTCCATTTGTTCCTGCTTCCGAGATCCCCAGGGCAGCCTGGATCCCTGC CCTTCTCAAGACTTTAGCTTTTCCTTCCATCCGGTGGCCTATTCCAGGAATTCCTCTTTTGCTTAAATCAGTTGGAGTTTGTGTCTGTTGCTTGTAATCA AGCCTTTATGGCTGCTGGGCTGAGTGACACAAGCACTTTAATGGCCTGGAGGGACTTTTAATCAGTGAAGATGCAATCAGACAAGTGTTTTGGAAAGAGC ACCCTCGAGAAGGGTGGATGACAGGGCAGAGCAGGAAGGACAGGAAGCTGGCAGAACGGAGGAGGCTGCAGCCGTGGTCCAACCAGGAGCTGATGGCAGC TGGGGCTAGGGGAAGGGCTTTGAGGGTGGAAGGATGGGATGGGTTCCAGAGGTATTCCTCTCTTAAATGCAAGTGCCTAGATTAGGTAGACTTTGCTTAG TATTGACAACTGCACATGAAAGTTTTGCAAAGGGAAACAGGCTAAATGCACCAAGAAAGCTTCTTCAGAGTGAAGAATCTTAATGCTTGTAATTTAAACA TTTGTTCCTGGAGTTTTGATTTGGTGGATGTGATGGTTGGTTTTATTTGTCAGTTTGGTTGGGCTATAGCACACAGTTATTTAATCAAACAGTAATCTAG GTGTGGCTGTGAAGGTATTTTGTAGATGTGATTAACATCTACAATCAGTTGACTTTAAGTGAAAGAGATTACTTAAATAATTTGGGTGAGCTGCACCTGA TTAGTTGAAAGGCCTCAAGAACAAACACTGCAGTTTCCTGGAAAAGAAGAAACTTTGCCTCAAGACTATAGCCATCGACTCCTGCCTGAGTTTCCAGCCT GCTAGTCTGCCCTATGGATTTGAAGTTTGCCAACCCCAACAATTGTGTGAATTAATTTCTAAAAATAAAGCTATATACAGCCATCCTTTGGTATTTGTGG GGGATTTGTTTCAGGATCTCTACAGATACCAAAATCTGTGGTTCCTCAAGTCCTTTATATGAAGTGGTATAGTATTTGCATATAACCTATGTGCAATCTC CTGTATAACTTCAATCATCTCTAGATTACTTATAATACCTAATACAGTGTAAATGCTAGTTGTTATACTGT

PTGS1.u.f.15 (forward primer)
PTGS1.u.r.17 (reverse primer)
Alternative donor

Frame 0

R*QLEGGAGVEPGEGWGGDGLELRAVCEAHAQE PALCVPHPSSRAMSRSLLLWFLLFLLLLPPLPVL LADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQ CDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFT HFLLTHGRWFWEFVNATFIREMLMRLVLTVRSNL IPSPPTYNSAHDYISWESFSNVSYYTRILPSVP KDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIP DPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKA LGHGVDLGHIYGDNLERQYQLRLFKDGKLKYQV LDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQ EVFGLLPGLMLYATLWLREHNRVCDLLKAEHPTW GDEQLFQTTRLILIGETIKIVIEEYVQQLSGYF LQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHP LMPDSFKIGGGRNMDHHILHVAVDVIRESREMRL QPFNEYRKRFGMKPYTSFQELVGEKEMAAELEE LYGDIDALEFYPGLLLEKCHPNSIFGESMIEIG APFSLKGLLGNPICSPEYWKPSTFGGEVGFNIVK TATLKKLVCLNTKTCPYVSFRVPDASQDDGPAV ERPSTEL*GAGKQHSGGESFVLVIPEC*GQG*W S*MLIFWFGMVSVGVDI*NFKSHPLSGIL*FCLF FQNAELLVSPSDC*EWFSFGLPEYWVLS*QPRM SDFWLICNTVILGCGATDEIC*KVRGFLFCIPE S*LSDW*FKVLCSWLMIQNSGSYPKSVSIWLSRM WI*FIFLFSEIS*RRRS*GPTRMHSLNLCLH*E GKEVGCSSWDPH*DPGLRM*REQVGCIHAIGWK LPELYPHPGLDSWQLFLMKLIKFAF*SYLLYISF GPIL*SRGNTGTWLAFLVAMAGRARGCSMRLSA GILGPITV*TCYHWYFLSPCGPGTALFRKLS*N THCPRPGRYNIQFPPSD*NNFLPYRAYNRGGTRG GEHILCSNFTLLILICSHTNSVKCVV*SPFQNQ GSSLRVVE*HTSRRLSPELVLLLIPGLTQFQA* SCLSGSGSKTEWWSVAST*YSGYSFSPKYVFS*V FTMCQFL*QVWGHSSE*SIQTRFCPYGAHTPVA DKQTINKETMK*DIYKVSVTSLLTPSALSSPIA LSRPETQQQ*SQGRQPSTPALRPFSQDLCRQQRE RTEG*DEGLREGSS*STLWA*YFSGRAMAQKQQ GKLEFGLTVLGVKSPVLWSRNSCFSLVGWKLLP SFLLASAECRECNPSLLLQLL*RRKILRVLEVSM KSLCPVLSCRFYFPSSANTSYFA*ESSPFLLIS VLVVLSGVHSGQGVGL*INPGQSDTLSLKTGPW RLRSLTLTHPHSWL*QDGPFVPASEIPRAAWIPA LLKTLAFPSIRWPIPGIPLLLKSVGVCVCCL*S SLYGCWAE*HKHFNGLEGLLISEDAIRQVFWKE HPREGWMTGQSRKDRKLAERRRLQPWSNQELMAA GARGRALRVEGWDGFQRYSSLKCKCLD*VDFA* Y*QLHMKVLQRETG*MHQESFFRVKNLNACNLN ICSWSFDLVDVMVGFICQFGWAIAHSYLIKQ*SR CGCEGIL*M*LTSTIS*L*VKEIT*IIWVSCT* LVERPQEQTLQFPGKEETLPQDYSHRLLPEFPA C*SALWI*SLPTPTIV*INF*K*SYIQPSFGICG GFVSGSLQIPKSVVPQVLYMKWYSICI*PMCNL LYNFNHL*ITYNT*YSVNASCYT

Frame 1

GDSWREERGWSRGKGGEGMGWSSGQCARRTHRS LHSASRTPAAAP*AGVSCSGSCCSCSCSRRSPS CSRTQGRPRQ*IPVVTIHASTRASVSASALTATS VTAPARAIPAPTAPSLACGPGSGIHCGPAPLSP TSCSLTGAGSGSLSMPPSSERCSCAWYSQCAPT LSPVPPPTTQHMTTSAGSLSPT*AITLVFCPLCL KIAPHPWEPKGRSSCQMPSSWPAASCSGGSSYL TPKAPTSCLPSLHNTSPTSSSKLLARWVLASPR PWAMG*TSATFMETIWSVSINCGSLRMGNSSTRC WMEKCTRPR*KRRLC*CTTPEASRPRARWLWAR RCLGCFLGSCCMPRSGYVSTTVCVTC*RLSTPP GAMSSFSRRPASSS*GRPSRLSSRSTCSS*VAIS CS*NLTQSCCSVSSSNTATALPWSSTISTTGTP SCLTPSRSVGAGTWTTTSCMWLWMSSGSLGRCG CSPSMSTARGLA*NPTPPSRSS*ERRRWQQSWRN CMETLMRWSSTLDCFLKSAIQTLSLGRV**RLG LPFPSRVS*GIPSVLRSTGSRAHLAARWALTLS RRPH*RSWSASTPRPVPTFPSVCRMPVRMMGLLW SDHPQSSEGQESSILEGRALCLSFQSAEARADG LKCSFSGLAW*VLGLTFRTLSLTHYLEYCDSVY SSRMLNSLLALQIVRSGSHLVCQNTGFLVDNLEC QISG*FVTQSF*DVELLMKSARKLGGSYFAFQN LDFLIGDSKCCVPG**SRTVARIPNLSASGCLE CGFDSFSCSVRYHRDGDPKVQQECIP*ICACTER ARKWGVLLGTPTKTLV*GCRENRWAVFTPLVGS YQSSIPIQVLTHGSCFS*S**NSLSKVTCYISL LVPSSKAEATLEHG*PFL*PWLGVLEVAA*DFLL GSLGPSLYRHATTGTSFLPAGQALPFSGSSLKI PIAPDLEDITFSSHHLIKTTSSLTEHTTEGAPG EESTYCVPISRF*FSFVLTPTV*SAWYNLHFKTK EAASEWSSDTPHAG*VQSLCSS*FLV*LSSRPD LACLAQGQRQNGGV*PPPDIQATHSVPNMYFPK CLLCASSCNRCGDTAVSNQYRQGSALMELTLQWQ TNRP*IRKR*NKIYTR*V*LPF*PPLLCPPLLR SQDQRPNSSDLRADSPPLQL*DPFLRTSVGSRE RGQRGKMRG*GKVLHDPHFGLSISQEELWPRNNR GN*SSV*QSLGLSLLSYGPETPVSP*LAGNCSH HSFWPLLNAGNAILPCSCSCSDVERSFGCWKSP *RACVLSFLADSISPLLLIPLTLLENPLLSY*FQ SWWFYQGCILAKGWACESILGNQTPSP*KLARG D*DH*L*LIPTAGSDKMVHLFLLPRSPGQPGSL PFSRL*LFLPSGGLFQEFLFCLNQLEFVSVACNQ AFMAAGLSDTSTLMAWRDF*SVKMQSDKCFGKS TLEKGG*QGRAGRTGSWQNGGGCSRGPTRS*WQ LGLGEGL*GWKDGMGSRGIPLLNASA*IR*TLLS IDNCT*KFCKGKQAKCTKKASSE*RILMLVI*T FVPGVLIWWM*WLVLFVSLVGL*HTVI*SNSNL GVAVKVFCRCD*HLQSVDFK*KRLLK*FG*AAPD *LKGLKNKHCSFLEKKKLCLKTIAIDSCLSFQP ASLPYGFEVCQPQQLCELISKNKAIYSHPLVFV GDLFQDLYRYQNLWFLKSFI*SGIVFAYNLCAIS CITSIISRLLIIPNTV*MLVVIL

Frame 2

VTAGGRSGGGAGGRVGRGWAGAPGSVRGARTGA CTLRPAPQQPRHEPESLALVLAVPAPAPAAPRP ARGPRGAHASESLLLLSMPAPGHLCPLRP*PLP V*LHPHGLFRPQLHHPWPVDLAPEFTAAQPLFHP LPAHSRALVLGVCQCHLHPRDAHAPGTHSALQP YPQSPHLQLST*LHQLGVFLQRELLHSYSALCA *RLPHTHGNQREEAVARCPAPGPPLPAQEEVHT* PPRHQPHVCLLCTTLHPPVLQNFWQDGSWLHQG LGPWGRPRPHLWRQSGASVSTAAL*GWETQVPG AGWRNVPALGRRGACVDALPPRHPAPEPDGCGPG GVWAASWAHAVCHALAT*AQPCV*PAEG*APHL GR*AAFPDDPPHPHRGDHQDCHRGVRAAAEWLF PAAEI*PRAAVRCPVPIPQPHCHGVQPSLPLAPP HA*LLQDRWGQEHGPPHPACGCGCHQGVSGDAA AALQ*VPQEVWHETLHLLPGARRREGDGSRVGG IVWRH*CVGVLPWTAS*KVPSKLYLWGEYDRDWG SLFPQGSPRESHLFSGVLEAEHIWRRGGL*HCQ DGHTEEAGLPQHQDLSLRFLPCAGCQSG*WACC GATIHRALRGRKAAFWRGELCACHSRVLRPGLMV LNAHFLVWHGECWG*HLEL*VSPIIWNIVILFI LPEC*TPC*PFRLLGVVLIWSARILGS*LTT*N VRFLVDL*HSHSRMWSY**NLLES*GVLILHSRI LTF*LVIQSVVFLADDPEQWLVSQICQHLAV*N VDLIHFPVQ*DIIETEILRSNKNAFPESVPALR GQGSGVFFLGPPLRPWSEDVERTGGLYSRHWLEA TRALSPSRS*LMAAVSHEANKIRFLKLPVIYLF WSHPLKQRQHWNMASLSCSHGWAC*RLQHETFC WDPWAHHCIDMLPLVLPFSLRARHCPFQEALLKY PLPQTWKI*HSVPTI*LKQLPPLQSIQQRGHPG RRAHTVFQFHAFNSHLFSHQQCEVRGIISISKP RKQPQSGRVTHLTQAESRACAPLDSWFDSVPGLI LPVWLRVKDRMVECSLHLIFRLLIQSQICIFLS VYYVPVPVTGVGTQQ*VINTDKVLPLWSSHSSG RQTDHK*GNDEIRYIQGECDFPSNPLCSVLPYCA LKTRDPTAVISGQTALHSSSETLFSGPL*AAER EDRGVR*GVEGRFFMIHTLGLVFLRKSYGPETT GETRVRSDSPWG*VSCLMVQKLLFLLSWLETAPI IPSGLC*MQGMQSFPALAVALT*KDPSGAGSLH EELVSCPFLQILFPLFC*YLLLCLRILSFLINF SLGGSIRGAFWPRGGPVNQSWAIRHPLLKNWPVE TEITDSDSSPQLALTRWSICSCFRDPQGSLDPC PSQDFSFSFHPVAYSRNSSFA*ISWSLCLLLVI KPLWLLG*VTQAL*WPGGTFNQ*RCNQTSVLERA PSRRVDDRAEQEGQEAGRTEEAAAVVQPGADGS WG*GKGFEGGRMGWVPEVFLS*MQVPRLGRLCL VLTTAHESFAKGNRLNAPRKLLQSEES*CL*FKH LFLEF*FGGCDGWFYLSVWLGYSTQLFNQTVI* VWL*RYFVDVINIYNQLTLSERDYLNNLGELHL IS*KASRTNTAVSWKRRNFASRL*PSTPA*VSSL LVCPMDLKFANPNNCVN*FLKIKLYTAILWYLW GICFRISTDTKICGSSSPLYEVV*YLHITYVQS PV*LQSSLDYL*YLIQCKC*LLYC

Sequence PTGS1.dAug05

AGGTGACAGCTGGAGGGAGGAGCGGGGGTGGAGCCGGGGGAAGGGTGGGGAGGGGATGGGCTGGAGCTCCGGGCAGTGTGCGAGGCGCACGCACAGGAGC CTGCACTCTGCGTCCCGCACCCCAGCAGCCGCGCCATGAGCCGGAGTCTCTTGCTCTGGTTCTTGCTGTTCCTGCTCCTGCTCCCGCCGCTCCCCGTCCT GCTCGCGGACCCAGGGGCGCCCACGCCAGTGAATCCCTGTTGTTACTATCCATGCCAGCACCAGGGCATCTGTGTCCGCTTCGGCCTTGACCGCTACCAG TGTGACTGCACCCGCACGGGCTATTCCGGCCCCAACTGCACCATCCCTGGCCTGTGGACCTGGCTCCGGAATTCACTGCGGCCCAGCCCCTCTTTCACCC ACTTCCTGCTCACTCACGGGCGCTGGTTCTGGGAGTTTGTCAATGCCACCTTCATCCGAGAGATGCTCATGCGCCTGGTACTCACAGGGAAGAAGCAGTT GCCAGATGCCCAGCTCCTGGCCCGCCGCTTCCTGCTCAGGAGGAAGTTCATACCTGACCCCCAAGGCACCAACCTCATGTTTGCCTTCTTTGCACAACAC TTCACCCACCAGTTCTTCAAAACTTCTGGCAAGATGGGTCCTGGCTTCACCAAGGCCTTGGGCCATGGGGTAGACCTCGGCCACATTTATGGAGACAATC TGGAGCGTCAGTATCAACTGCGGCTCTTTAAGGATGGGAAACTCAAGTACCAGGTGCTGGATGGAGAAATGTACCCGCCCTCGGTAGAAGAGGCGCCTGT GTTGATGCACTACCCCCGAGGCATCCCGCCCCAGAGCCAGATGGCTGTGGGCCAGGAGGTGTTTGGGCTGCTTCCTGGGCTCATGCTGTATGCCACGCTC TGGCTACGTGAGCACAACCGTGTGTGTGACCTGCTGAAGGCTGAGCACCCCACCTGGGGCGATGAGCAGCTTTTCCAGACGACCCGCCTCATCCTCATAG GGGAGACCATCAAGATTGTCATCGAGGAGTACGTGCAGCAGCTGAGTGGCTATTTCCTGCAGCTGAAATTTGACCCAGAGCTGCTGTTCGGTGTCCAGTT CCAATACCGCAACCGCATTGCCATGGAGTTCAACCATCTCTACCACTGGCACCCCCTCATGCCTGACTCCTTCAAGGTGGGCTCCCAGGAGTACAGCTAC GAGCAGTTCTTGTTCAACACCTCCATGTTGGTGGACTATGGGGTTGAGGCCCTGGTGGATGCCTTCTCTCGCCAGATTGCTGGCCGGATCGGTGGGGGCA GGAACATGGACCACCACATCCTGCATGTGGCTGTGGATGTCATCAGGGAGTCTCGGGAGATGCGGCTGCAGCCCTTCAATGAGTACCGCAAGAGGTTTGG CATGAAACCCTACACCTCCTTCCAGGAGCTCGTAGGAGAGAAGGAGATGGCAGCAGAGTTGGAGGAATTGTATGGAGACATTGATGCGTTGGAGTTCTAC CCTGGACTGCTTCTTGAAAAGTGCCATCCAAACTCTATCTTTGGGGAGAGTATGATAGAGATTGGGGCTCCCTTTTCCCTCAAGGGTCTCCTAGGGAATC CCATCTGTTCTCCGGAGTACTGGAAGCCGAGCACATTTGGCGGCGAGGTGGGCTTTAACATTGTCAAGACGGCCACACTGAAGAAGCTGGTCTGCCTCAA CACCAAGACCTGTCCCTACGTTTCCTTCCGTGTGCCGGATGCCAGTCAGGATGATGGGCCTGCTGTGGAGCGACCATCCACAGAGCTCTGAGGGGCAGGA AAGCAGCATTCTGGAGGGGAGAGCTTTGTGCTTGTCATTCCAGAGTGCTGAGGCCAGGGCTGATGGTCTTAAATGCTCATTTTCTGGTTTGGCATGGTGA GTGTTGGGGTTGACATTTAGAACTTTAAGTCTCACCCATTATCTGGAATATTGTGATTCTGTTTATTCTTCCAGAATGCTGAACTCCTTGTTAGCCCTTC AGATTGTTAGGAGTGGTTCTCATTTGGTCTGCCAGAATACTGGGTTCTTAGTTGACAACCTAGAATGTCAGATTTCTGGTTGATTTGTAACACAGTCATT CTAGGATGTGGAGCTACTGATGAAATCTGCTAGAAAGTTAGGGGGTTCTTATTTTGCATTCCAGAATCTTGACTTTCTGATTGGTGATTCAAAGTGTTGT GTTCCTGGCTGATGATCCAGAACAGTGGCTCGTATCCCAAATCTGTCAGCATCTGGCTGTCTAGAATGTGGATTTGATTCATTTTCCTGTTCAGTGAGAT ATCATAGAGACGGAGATCCTAAGGTCCAACAAGAATGCATTCCCTGAATCTGTGCCTGCACTGAGAGGGCAAGGAAGTGGGGTGTTCTTCTTGGGACCCC CACTAAGACCCTGGTCTGAGGATGTAGAGAGAACAGGTGGGCTGTATTCACGCCATTGGTTGGAAGCTACCAGAGCTCTATCCCCATCCAGGTCTTGACT CATGGCAGCTGTTTCTCATGAAGCTAATAAAATTCGCTTTCTAAAGTTACCTGTTATATATCTCTTTTGGTCCCATCCTCTAAAGCAGAGGCAACACTGG AACATGGCTAGCCTTTCTTGTAGCCATGGCTGGGCGTGCTAGAGGTTGCAGCATGAGACTTTCTGCTGGGATCCTTGGGCCCATCACTGTATAGACATGC TACCACTGGTACTTCCTTTCTCCCTGCGGGCCAGGCACTGCCCTTTTCAGGAAGCTCTCTTAAAATACCCATTGCCCCAGACCTGGAAGATATAACATTC AGTTCCCACCATCTGATTAAAACAACTTCCTCCCTTACAGAGCATACAACAGAGGGGGCACCCGGGGAGGAGAGCACATACTGTGTTCCAATTTCACGCT TTTAATTCTCATTTGTTCTCACACCAACAGTGTGAAGTGCGTGGTATAATCTCCATTTCAAAACCAAGGAAGCAGCCTCAGAGTGGTCGAGTGACACACC TCACGCAGGCTGAGTCCAGAGCTTGTGCTCCTCTTGATTCCTGGTTTGACTCAGTTCCAGGCCTGATCTTGCCTGTCTGGCTCAGGGTCAAAGACAGAAT GGTGGAGTGTAGCCTCCACCTGATATTCAGGCTACTCATTCAGTCCCAAATATGTATTTTCCTAAGTGTTTACTATGTGCCAGTTCCTGTAACAGGTGTG GGGACACAGCAGTGAGTAATCAATACAGACAAGGTTCTGCCCTTATGGAGCTCACACTCCAGTGGCAGACAAACAGACCATAAATAAGGAAACGATGAAA TAAGATATATACAAGGTGAGTGTGACTTCCCTTCTAACCCCCTCTGCTCTGTCCTCCCCTATTGCGCTCTCAAGACCAGAGACCCAACAGCAGTGATCTC AGGGCAGACAGCCCTCCACTCCAGCTCTGAGACCCTTTTCTCAGGACCTCTGTAGGCAGCAGAGAGAGAGGACAGAGGGGTAAGATGAGGGGTTGAGGGA AGGTTCTTCATGATCCACACTTTGGGCTTAGTATTTCTCAGGAAGAGCTATGGCCCAGAAACAACAGGGGAAACTAGAGTTCGGTCTGACAGTCCTTGGG GTTAAGTCTCCTGTCTTATGGTCCAGAAACTCCTGTTTCTCCTTAGTTGGCTGGAAACTGCTCCCATCATTCCTTCTGGCCTCTGCTGAATGCAGGGAAT GCAATCCTTCCCTGCTCTTGCAGTTGCTCTGACGTAGAAAGATCCTTCGGGTGCTGGAAGTCTCCATGAAGAGCTTGTGTCCTGTCCTTTCTTGCAGATT CTATTTCCCCTCTTCTGCTAATACCTCTTACTTTGCTTGAGAATCCTCTCCTTTCTTATTAATTTCAGTCTTGGTGGTTCTATCAGGGGTGCATTCTGGC CAAGGGGTGGGCCTGTGAATCAATCCTGGGCAATCAGACACCCTCTCCTTAAAAACTGGCCCGTGGAGACTGAGATCACTGACTCTGACTCATCCCCACA GCTGGCTCTGACAAGATGGTCCATTTGTTCCTGCTTCCGAGATCCCCAGGGCAGCCTGGATCCCTGCCCTTCTCAAGACTTTAGCTTTTCCTTCCATCCG GTGGCCTATTCCAGGAATTCCTCTTTTGCTTAAATCAGTTGGAGTTTGTGTCTGTTGCTTGTAATCAAGCCTTTATGGCTGCTGGGCTGAGTGACACAAG CACTTTAATGGCCTGGAGGGACTTTTAATCAGTGAAGATGCAATCAGACAAGTGTTTTGGAAAGAGCACCCTCGAGAAGGGTGGATGACAGGGCAGAGCA GGAAGGACAGGAAGCTGGCAGAACGGAGGAGGCTGCAGCCGTGGTCCAACCAGGAGCTGATGGCAGCTGGGGCTAGGGGAAGGGCTTTGAGGGTGGAAGG ATGGGATGGGTTCCAGAGGTATTCCTCTCTTAAATGCAAGTGCCTAGATTAGGTAGACTTTGCTTAGTATTGACAACTGCACATGAAAGTTTTGCAAAGG GAAACAGGCTAAATGCACCAAGAAAGCTTCTTCAGAGTGAAGAATCTTAATGCTTGTAATTTAAACATTTGTTCCTGGAGTTTTGATTTGGTGGATGTGA TGGTTGGTTTTATTTGTCAGTTTGGTTGGGCTATAGCACACAGTTATTTAATCAAACAGTAATCTAGGTGTGGCTGTGAAGGTATTTTGTAGATGTGATT AACATCTACAATCAGTTGACTTTAAGTGAAAGAGATTACTTAAATAATTTGGGTGAGCTGCACCTGATTAGTTGAAAGGCCTCAAGAACAAACACTGCAG TTTCCTGGAAAAGAAGAAACTTTGCCTCAAGACTATAGCCATCGACTCCTGCCTGAGTTTCCAGCCTGCTAGTCTGCCCTATGGATTTGAAGTTTGCCAA CCCCAACAATTGTGTGAATTAATTTCTAAAAATAAAGCTATATACAGCCATCCTTTGGTATTTGTGGGGGATTTGTTTCAGGATCTCTACAGATACCAAA ATCTGTGGTTCCTCAAGTCCTTTATATGAAGTGGTATAGTATTTGCATATAACCTATGTGCAATCTCCTGTATAACTTCAATCATCTCTAGATTACTTAT AATACCTAATACAGTGTAAATGCTAGTTGTTATACTGT

PTGS1.u.f.15 (forward primer)
PTGS1.u.r.17 (reverse primer)
Alternative donor

Frame 0

R*QLEGGAGVEPGEGWGGDGLELRAVCEAHAQE PALCVPHPSSRAMSRSLLLWFLLFLLLLPPLPVL LADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQ CDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFT HFLLTHGRWFWEFVNATFIREMLMRLVLTGKKQL PDAQLLARRFLLRRKFIPDPQGTNLMFAFFAQH FTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDN LERQYQLRLFKDGKLKYQVLDGEMYPPSVEEAPV LMHYPRGIPPQSQMAVGQEVFGLLPGLMLYATL WLREHNRVCDLLKAEHPTWGDEQLFQTTRLILI GETIKIVIEEYVQQLSGYFLQLKFDPELLFGVQF QYRNRIAMEFNHLYHWHPLMPDSFKVGSQEYSY EQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGG RNMDHHILHVAVDVIRESREMRLQPFNEYRKRFG MKPYTSFQELVGEKEMAAELEELYGDIDALEFY PGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGN PICSPEYWKPSTFGGEVGFNIVKTATLKKLVCLN TKTCPYVSFRVPDASQDDGPAVERPSTEL*GAG KQHSGGESFVLVIPEC*GQG*WS*MLIFWFGMV SVGVDI*NFKSHPLSGIL*FCLFFQNAELLVSPS DC*EWFSFGLPEYWVLS*QPRMSDFWLICNTVI LGCGATDEIC*KVRGFLFCIPES*LSDW*FKVL CSWLMIQNSGSYPKSVSIWLSRMWI*FIFLFSEI S*RRRS*GPTRMHSLNLCLH*EGKEVGCSSWDP H*DPGLRM*REQVGCIHAIGWKLPELYPHPGLD SWQLFLMKLIKFAF*SYLLYISFGPIL*SRGNTG TWLAFLVAMAGRARGCSMRLSAGILGPITV*TC YHWYFLSPCGPGTALFRKLS*NTHCPRPGRYNI QFPPSD*NNFLPYRAYNRGGTRGGEHILCSNFTL LILICSHTNSVKCVV*SPFQNQGSSLRVVE*HT SRRLSPELVLLLIPGLTQFQA*SCLSGSGSKTE WWSVAST*YSGYSFSPKYVFS*VFTMCQFL*QVW GHSSE*SIQTRFCPYGAHTPVADKQTINKETMK *DIYKVSVTSLLTPSALSSPIALSRPETQQQ*S QGRQPSTPALRPFSQDLCRQQRERTEG*DEGLRE GSS*STLWA*YFSGRAMAQKQQGKLEFGLTVLG VKSPVLWSRNSCFSLVGWKLLPSFLLASAECRE CNPSLLLQLL*RRKILRVLEVSMKSLCPVLSCRF YFPSSANTSYFA*ESSPFLLISVLVVLSGVHSG QGVGL*INPGQSDTLSLKTGPWRLRSLTLTHPH SWL*QDGPFVPASEIPRAAWIPALLKTLAFPSIR WPIPGIPLLLKSVGVCVCCL*SSLYGCWAE*HK HFNGLEGLLISEDAIRQVFWKEHPREGWMTGQS RKDRKLAERRRLQPWSNQELMAAGARGRALRVEG WDGFQRYSSLKCKCLD*VDFA*Y*QLHMKVLQR ETG*MHQESFFRVKNLNACNLNICSWSFDLVDV MVGFICQFGWAIAHSYLIKQ*SRCGCEGIL*M*L TSTIS*L*VKEIT*IIWVSCT*LVERPQEQTLQ FPGKEETLPQDYSHRLLPEFPAC*SALWI*SLP TPTIV*INF*K*SYIQPSFGICGGFVSGSLQIPK SVVPQVLYMKWYSICI*PMCNLLYNFNHL*ITY NT*YSVNASCYT

Frame 1

GDSWREERGWSRGKGGEGMGWSSGQCARRTHRS LHSASRTPAAAP*AGVSCSGSCCSCSCSRRSPS CSRTQGRPRQ*IPVVTIHASTRASVSASALTATS VTAPARAIPAPTAPSLACGPGSGIHCGPAPLSP TSCSLTGAGSGSLSMPPSSERCSCAWYSQGRSS CQMPSSWPAASCSGGSSYLTPKAPTSCLPSLHNT SPTSSSKLLARWVLASPRPWAMG*TSATFMETI WSVSINCGSLRMGNSSTRCWMEKCTRPR*KRRL C*CTTPEASRPRARWLWARRCLGCFLGSCCMPRS GYVSTTVCVTC*RLSTPPGAMSSFSRRPASSS* GRPSRLSSRSTCSS*VAISCS*NLTQSCCSVSS SNTATALPWSSTISTTGTPSCLTPSRWAPRSTAT SSSCSTPPCWWTMGLRPWWMPSLARLLAGSVGA GTWTTTSCMWLWMSSGSLGRCGCSPSMSTARGL A*NPTPPSRSS*ERRRWQQSWRNCMETLMRWSST LDCFLKSAIQTLSLGRV**RLGLPFPSRVS*GI PSVLRSTGSRAHLAARWALTLSRRPH*RSWSAS TPRPVPTFPSVCRMPVRMMGLLWSDHPQSSEGQE SSILEGRALCLSFQSAEARADGLKCSFSGLAW* VLGLTFRTLSLTHYLEYCDSVYSSRMLNSLLAL QIVRSGSHLVCQNTGFLVDNLECQISG*FVTQSF *DVELLMKSARKLGGSYFAFQNLDFLIGDSKCC VPG**SRTVARIPNLSASGCLECGFDSFSCSVR YHRDGDPKVQQECIP*ICACTERARKWGVLLGTP TKTLV*GCRENRWAVFTPLVGSYQSSIPIQVLT HGSCFS*S**NSLSKVTCYISLLVPSSKAEATL EHG*PFL*PWLGVLEVAA*DFLLGSLGPSLYRHA TTGTSFLPAGQALPFSGSSLKIPIAPDLEDITF SSHHLIKTTSSLTEHTTEGAPGEESTYCVPISR F*FSFVLTPTV*SAWYNLHFKTKEAASEWSSDTP HAG*VQSLCSS*FLV*LSSRPDLACLAQGQRQN GGV*PPPDIQATHSVPNMYFPKCLLCASSCNRC GDTAVSNQYRQGSALMELTLQWQTNRP*IRKR*N KIYTR*V*LPF*PPLLCPPLLRSQDQRPNSSDL RADSPPLQL*DPFLRTSVGSRERGQRGKMRG*G KVLHDPHFGLSISQEELWPRNNRGN*SSV*QSLG LSLLSYGPETPVSP*LAGNCSHHSFWPLLNAGN AILPCSCSCSDVERSFGCWKSP*RACVLSFLAD SISPLLLIPLTLLENPLLSY*FQSWWFYQGCILA KGWACESILGNQTPSP*KLARGD*DH*L*LIPT AGSDKMVHLFLLPRSPGQPGSLPFSRL*LFLPS GGLFQEFLFCLNQLEFVSVACNQAFMAAGLSDTS TLMAWRDF*SVKMQSDKCFGKSTLEKGG*QGRA GRTGSWQNGGGCSRGPTRS*WQLGLGEGL*GWK DGMGSRGIPLLNASA*IR*TLLSIDNCT*KFCKG KQAKCTKKASSE*RILMLVI*TFVPGVLIWWM* WLVLFVSLVGL*HTVI*SNSNLGVAVKVFCRCD *HLQSVDFK*KRLLK*FG*AAPD*LKGLKNKHCS FLEKKKLCLKTIAIDSCLSFQPASLPYGFEVCQ PQQLCELISKNKAIYSHPLVFVGDLFQDLYRYQ NLWFLKSFI*SGIVFAYNLCAISCITSIISRLLI IPNTV*MLVVIL

Frame 2

VTAGGRSGGGAGGRVGRGWAGAPGSVRGARTGA CTLRPAPQQPRHEPESLALVLAVPAPAPAAPRP ARGPRGAHASESLLLLSMPAPGHLCPLRP*PLP V*LHPHGLFRPQLHHPWPVDLAPEFTAAQPLFHP LPAHSRALVLGVCQCHLHPRDAHAPGTHREEAV ARCPAPGPPLPAQEEVHT*PPRHQPHVCLLCTT LHPPVLQNFWQDGSWLHQGLGPWGRPRPHLWRQS GASVSTAAL*GWETQVPGAGWRNVPALGRRGAC VDALPPRHPAPEPDGCGPGGVWAASWAHAVCHA LAT*AQPCV*PAEG*APHLGR*AAFPDDPPHPHR GDHQDCHRGVRAAAEWLFPAAEI*PRAAVRCPV PIPQPHCHGVQPSLPLAPPHA*LLQGGLPGVQL RAVLVQHLHVGGLWG*GPGGCLLSPDCWPDRWGQ EHGPPHPACGCGCHQGVSGDAAAALQ*VPQEVW HETLHLLPGARRREGDGSRVGGIVWRH*CVGVL PWTAS*KVPSKLYLWGEYDRDWGSLFPQGSPRES HLFSGVLEAEHIWRRGGL*HCQDGHTEEAGLPQ HQDLSLRFLPCAGCQSG*WACCGATIHRALRGR KAAFWRGELCACHSRVLRPGLMVLNAHFLVWHGE CWG*HLEL*VSPIIWNIVILFILPEC*TPC*PF RLLGVVLIWSARILGS*LTT*NVRFLVDL*HSH SRMWSY**NLLES*GVLILHSRILTF*LVIQSVV FLADDPEQWLVSQICQHLAV*NVDLIHFPVQ*D IIETEILRSNKNAFPESVPALRGQGSGVFFLGP PLRPWSEDVERTGGLYSRHWLEATRALSPSRS*L MAAVSHEANKIRFLKLPVIYLFWSHPLKQRQHW NMASLSCSHGWAC*RLQHETFCWDPWAHHCIDM LPLVLPFSLRARHCPFQEALLKYPLPQTWKI*HS VPTI*LKQLPPLQSIQQRGHPGRRAHTVFQFHA FNSHLFSHQQCEVRGIISISKPRKQPQSGRVTH LTQAESRACAPLDSWFDSVPGLILPVWLRVKDRM VECSLHLIFRLLIQSQICIFLSVYYVPVPVTGV GTQQ*VINTDKVLPLWSSHSSGRQTDHK*GNDE IRYIQGECDFPSNPLCSVLPYCALKTRDPTAVIS GQTALHSSSETLFSGPL*AAEREDRGVR*GVEG RFFMIHTLGLVFLRKSYGPETTGETRVRSDSPW G*VSCLMVQKLLFLLSWLETAPIIPSGLC*MQGM QSFPALAVALT*KDPSGAGSLHEELVSCPFLQI LFPLFC*YLLLCLRILSFLINFSLGGSIRGAFW PRGGPVNQSWAIRHPLLKNWPVETEITDSDSSPQ LALTRWSICSCFRDPQGSLDPCPSQDFSFSFHP VAYSRNSSFA*ISWSLCLLLVIKPLWLLG*VTQ AL*WPGGTFNQ*RCNQTSVLERAPSRRVDDRAEQ EGQEAGRTEEAAAVVQPGADGSWG*GKGFEGGR MGWVPEVFLS*MQVPRLGRLCLVLTTAHESFAK GNRLNAPRKLLQSEES*CL*FKHLFLEF*FGGCD GWFYLSVWLGYSTQLFNQTVI*VWL*RYFVDVI NIYNQLTLSERDYLNNLGELHLIS*KASRTNTA VSWKRRNFASRL*PSTPA*VSSLLVCPMDLKFAN PNNCVN*FLKIKLYTAILWYLWGICFRISTDTK ICGSSSPLYEVV*YLHITYVQSPV*LQSSLDYL *YLIQCKC*LLYC

Sequence PTGS1.cAug05

AGGTGACAGCTGGAGGGAGGAGCGGGGGTGGAGCCGGGGGAAGGGTGGGGAGGGGATGGGCTGGAGCTCCGGGCAGTGTGCGAGGCGCACGCACAGGAGC CTGCACTCTGCGTCCCGCACCCCAGCAGCCGCGCCATGAGCCGGAGTCTCTTGCTCTGGTTCTTGCTGTTCCTGCTCCTGCTCCCGCCGCTCCCCGTCCT GCTCGCGGACCCAGGGGCGCCCACGCCAGGGCCTCTTTGGGAGGAAGCCGCAGGCACCAAGGGAAATGAGTTCCCTTTCTCCAGCCTCTAACCGTCTGGG AACCCATCCTGATTCCCATTGCCAGTGGAGAAGGTCTCCCCTGGTGAAGACTTCGGGAGAACATGGGAGATGGAAATACATTTAGGAGCCGGGATGCTTC ATCTGGGGTTTAAGAGATCCCCATTGAGCAAATGAGGAAACCGAGGCTCATGAATCCCTGTTGTTACTATCCATGCCAGCACCAGGGCATCTGTGTCCGC TTCGGCCTTGACCGCTACCAGTGTGACTGCACCCGCACGGGCTATTCCGGCCCCAACTGCACCATCCCTGGCCTGTGGACCTGGCTCCGGAATTCACTGC GGCCCAGCCCCTCTTTCACCCACTTCCTGCTCACTCACGGGCGCTGGTTCTGGGAGTTTGTCAATGCCACCTTCATCCGAGAGATGCTCATGCGCCTGGT ACTCACAGTGCGCTCCAACCTTATCCCCAGTCCCCCCACCTACAACTCAGCACATGACTACATCAGCTGGGAGTCTTTCTCCAACGTGAGCTATTACACT CGTATTCTGCCCTCTGTGCCTAAAGATTGCCCCACACCCATGGGAACCAAAGGGAAGAAGCAGTTGCCAGATGCCCAGCTCCTGGCCCGCCGCTTCCTGC TCAGGAGGAAGTTCATACCTGACCCCCAAGGCACCAACCTCATGTTTGCCTTCTTTGCACAACACTTCACCCACCAGTTCTTCAAAACTTCTGGCAAGAT GGGTCCTGGCTTCACCAAGGCCTTGGGCCATGGGGTAGACCTCGGCCACATTTATGGAGACAATCTGGAGCGTCAGTATCAACTGCGGCTCTTTAAGGAT GGGAAACTCAAGTACCAGGTGCTGGATGGAGAAATGTACCCGCCCTCGGTAGAAGAGGCGCCTGTGTTGATGCACTACCCCCGAGGCATCCCGCCCCAGA GCCAGATGGCTGTGGGCCAGGAGGTGTTTGGGCTGCTTCCTGGGCTCATGCTGTATGCCACGCTCTGGCTACGTGAGCACAACCGTGTGTGTGACCTGCT GAAGGCTGAGCACCCCACCTGGGGCGATGAGCAGCTTTTCCAGACGACCCGCCTCATCCTCATAGGGGAGACCATCAAGATTGTCATCGAGGAGTACGTG CAGCAGCTGAGTGGCTATTTCCTGCAGCTGAAATTTGACCCAGAGCTGCTGTTCGGTGTCCAGTTCCAATACCGCAACCGCATTGCCATGGAGTTCAACC ATCTCTACCACTGGCACCCCCTCATGCCTGACTCCTTCAAGGTGGGCTCCCAGGAGTACAGCTACGAGCAGTTCTTGTTCAACACCTCCATGTTGGTGGA CTATGGGGTTGAGGCCCTGGTGGATGCCTTCTCTCGCCAGATTGCTGGCCGGATCGGTGGGGGCAGGAACATGGACCACCACATCCTGCATGTGGCTGTG GATGTCATCAGGGAGTCTCGGGAGATGCGGCTGCAGCCCTTCAATGAGTACCGCAAGAGGTTTGGCATGAAACCCTACACCTCCTTCCAGGAGCTCGTAG GAGAGAAGGAGATGGCAGCAGAGTTGGAGGAATTGTATGGAGACATTGATGCGTTGGAGTTCTACCCTGGACTGCTTCTTGAAAAGTGCCATCCAAACTC TATCTTTGGGGAGAGTATGATAGAGATTGGGGCTCCCTTTTCCCTCAAGGGTCTCCTAGGGAATCCCATCTGTTCTCCGGAGTACTGGAAGCCGAGCACA TTTGGCGGCGAGGTGGGCTTTAACATTGTCAAGACGGCCACACTGAAGAAGCTGGTCTGCCTCAACACCAAGACCTGTCCCTACGTTTCCTTCCGTGTGC CGGATGCCAGTCAGGATGATGGGCCTGCTGTGGAGCGACCATCCACAGAGCTCTGAGGGGCAGGAAAGCAGCATTCTGGAGGGGAGAGCTTTGTGCTTGT CATTCCAGAGTGCTGAGGCCAGGGCTGATGGTCTTAAATGCTCATTTTCTGGTTTGGCATGGTGAGTGTTGGGGTTGACATTTAGAACTTTAAGTCTCAC CCATTATCTGGAATATTGTGATTCTGTTTATTCTTCCAGAATGCTGAACTCCTTGTTAGCCCTTCAGATTGTTAGGAGTGGTTCTCATTTGGTCTGCCAG AATACTGGGTTCTTAGTTGACAACCTAGAATGTCAGATTTCTGGTTGATTTGTAACACAGTCATTCTAGGATGTGGAGCTACTGATGAAATCTGCTAGAA AGTTAGGGGGTTCTTATTTTGCATTCCAGAATCTTGACTTTCTGATTGGTGATTCAAAGTGTTGTGTTCCTGGCTGATGATCCAGAACAGTGGCTCGTAT CCCAAATCTGTCAGCATCTGGCTGTCTAGAATGTGGATTTGATTCATTTTCCTGTTCAGTGAGATATCATAGAGACGGAGATCCTAAGGTCCAACAAGAA TGCATTCCCTGAATCTGTGCCTGCACTGAGAGGGCAAGGAAGTGGGGTGTTCTTCTTGGGACCCCCACTAAGACCCTGGTCTGAGGATGTAGAGAGAACA GGTGGGCTGTATTCACGCCATTGGTTGGAAGCTACCAGAGCTCTATCCCCATCCAGGTCTTGACTCATGGCAGCTGTTTCTCATGAAGCTAATAAAATTC GCTTTCTAAAGTTACCTGTTATATATCTCTTTTGGTCCCATCCTCTAAAGCAGAGGCAACACTGGAACATGGCTAGCCTTTCTTGTAGCCATGGCTGGGC GTGCTAGAGGTTGCAGCATGAGACTTTCTGCTGGGATCCTTGGGCCCATCACTGTATAGACATGCTACCACTGGTACTTCCTTTCTCCCTGCGGGCCAGG CACTGCCCTTTTCAGGAAGCTCTCTTAAAATACCCATTGCCCCAGACCTGGAAGATATAACATTCAGTTCCCACCATCTGATTAAAACAACTTCCTCCCT TACAGAGCATACAACAGAGGGGGCACCCGGGGAGGAGAGCACATACTGTGTTCCAATTTCACGCTTTTAATTCTCATTTGTTCTCACACCAACAGTGTGA AGTGCGTGGTATAATCTCCATTTCAAAACCAAGGAAGCAGCCTCAGAGTGGTCGAGTGACACACCTCACGCAGGCTGAGTCCAGAGCTTGTGCTCCTCTT GATTCCTGGTTTGACTCAGTTCCAGGCCTGATCTTGCCTGTCTGGCTCAGGGTCAAAGACAGAATGGTGGAGTGTAGCCTCCACCTGATATTCAGGCTAC TCATTCAGTCCCAAATATGTATTTTCCTAAGTGTTTACTATGTGCCAGTTCCTGTAACAGGTGTGGGGACACAGCAGTGAGTAATCAATACAGACAAGGT TCTGCCCTTATGGAGCTCACACTCCAGTGGCAGACAAACAGACCATAAATAAGGAAACGATGAAATAAGATATATACAAGGTGAGTGTGACTTCCCTTCT AACCCCCTCTGCTCTGTCCTCCCCTATTGCGCTCTCAAGACCAGAGACCCAACAGCAGTGATCTCAGGGCAGACAGCCCTCCACTCCAGCTCTGAGACCC TTTTCTCAGGACCTCTGTAGGCAGCAGAGAGAGAGGACAGAGGGGTAAGATGAGGGGTTGAGGGAAGGTTCTTCATGATCCACACTTTGGGCTTAGTATT TCTCAGGAAGAGCTATGGCCCAGAAACAACAGGGGAAACTAGAGTTCGGTCTGACAGTCCTTGGGGTTAAGTCTCCTGTCTTATGGTCCAGAAACTCCTG TTTCTCCTTAGTTGGCTGGAAACTGCTCCCATCATTCCTTCTGGCCTCTGCTGAATGCAGGGAATGCAATCCTTCCCTGCTCTTGCAGTTGCTCTGACGT AGAAAGATCCTTCGGGTGCTGGAAGTCTCCATGAAGAGCTTGTGTCCTGTCCTTTCTTGCAGATTCTATTTCCCCTCTTCTGCTAATACCTCTTACTTTG CTTGAGAATCCTCTCCTTTCTTATTAATTTCAGTCTTGGTGGTTCTATCAGGGGTGCATTCTGGCCAAGGGGTGGGCCTGTGAATCAATCCTGGGCAATC AGACACCCTCTCCTTAAAAACTGGCCCGTGGAGACTGAGATCACTGACTCTGACTCATCCCCACAGCTGGCTCTGACAAGATGGTCCATTTGTTCCTGCT TCCGAGATCCCCAGGGCAGCCTGGATCCCTGCCCTTCTCAAGACTTTAGCTTTTCCTTCCATCCGGTGGCCTATTCCAGGAATTCCTCTTTTGCTTAAAT CAGTTGGAGTTTGTGTCTGTTGCTTGTAATCAAGCCTTTATGGCTGCTGGGCTGAGTGACACAAGCACTTTAATGGCCTGGAGGGACTTTTAATCAGTGA AGATGCAATCAGACAAGTGTTTTGGAAAGAGCACCCTCGAGAAGGGTGGATGACAGGGCAGAGCAGGAAGGACAGGAAGCTGGCAGAACGGAGGAGGCTG CAGCCGTGGTCCAACCAGGAGCTGATGGCAGCTGGGGCTAGGGGAAGGGCTTTGAGGGTGGAAGGATGGGATGGGTTCCAGAGGTATTCCTCTCTTAAAT GCAAGTGCCTAGATTAGGTAGACTTTGCTTAGTATTGACAACTGCACATGAAAGTTTTGCAAAGGGAAACAGGCTAAATGCACCAAGAAAGCTTCTTCAG AGTGAAGAATCTTAATGCTTGTAATTTAAACATTTGTTCCTGGAGTTTTGATTTGGTGGATGTGATGGTTGGTTTTATTTGTCAGTTTGGTTGGGCTATA GCACACAGTTATTTAATCAAACAGTAATCTAGGTGTGGCTGTGAAGGTATTTTGTAGATGTGATTAACATCTACAATCAGTTGACTTTAAGTGAAAGAGA TTACTTAAATAATTTGGGTGAGCTGCACCTGATTAGTTGAAAGGCCTCAAGAACAAACACTGCAGTTTCCTGGAAAAGAAGAAACTTTGCCTCAAGACTA TAGCCATCGACTCCTGCCTGAGTTTCCAGCCTGCTAGTCTGCCCTATGGATTTGAAGTTTGCCAACCCCAACAATTGTGTGAATTAATTTCTAAAAATAA AGCTATATACAGCCATCCTTTGGTATTTGTGGGGGATTTGTTTCAGGATCTCTACAGATACCAAAATCTGTGGTTCCTCAAGTCCTTTATATGAAGTGGT ATAGTATTTGCATATAACCTATGTGCAATCTCCTGTATAACTTCAATCATCTCTAGATTACTTATAATACCTAATACAGTGTAAATGCTAGTTGTTATAC TGT

PTGS1.u.f.15 (forward primer)
PTGS1.u.r.17 (reverse primer)
Alternative donor

Frame 0

R*QLEGGAGVEPGEGWGGDGLELRAVCEAHAQE PALCVPHPSSRAMSRSLLLWFLLFLLLLPPLPVL LADPGAPTPGPLWEEAAGTKGNEFPFSSL*PSG NPS*FPLPVEKVSPGEDFGRTWEMEIHLGAGML HLGFKRSPLSK*GNRGS*IPVVTIHASTRASVSA SALTATSVTAPARAIPAPTAPSLACGPGSGIHC GPAPLSPTSCSLTGAGSGSLSMPPSSERCSCAW YSQCAPTLSPVPPPTTQHMTTSAGSLSPT*AITL VFCPLCLKIAPHPWEPKGRSSCQMPSSWPAASC SGGSSYLTPKAPTSCLPSLHNTSPTSSSKLLAR WVLASPRPWAMG*TSATFMETIWSVSINCGSLRM GNSSTRCWMEKCTRPR*KRRLC*CTTPEASRPR ARWLWARRCLGCFLGSCCMPRSGYVSTTVCVTC *RLSTPPGAMSSFSRRPASSS*GRPSRLSSRSTC SS*VAISCS*NLTQSCCSVSSSNTATALPWSST ISTTGTPSCLTPSRWAPRSTATSSSCSTPPCWW TMGLRPWWMPSLARLLAGSVGAGTWTTTSCMWLW MSSGSLGRCGCSPSMSTARGLA*NPTPPSRSS* ERRRWQQSWRNCMETLMRWSSTLDCFLKSAIQT LSLGRV**RLGLPFPSRVS*GIPSVLRSTGSRAH LAARWALTLSRRPH*RSWSASTPRPVPTFPSVC RMPVRMMGLLWSDHPQSSEGQESSILEGRALCL SFQSAEARADGLKCSFSGLAW*VLGLTFRTLSLT HYLEYCDSVYSSRMLNSLLALQIVRSGSHLVCQ NTGFLVDNLECQISG*FVTQSF*DVELLMKSAR KLGGSYFAFQNLDFLIGDSKCCVPG**SRTVARI PNLSASGCLECGFDSFSCSVRYHRDGDPKVQQE CIP*ICACTERARKWGVLLGTPTKTLV*GCREN RWAVFTPLVGSYQSSIPIQVLTHGSCFS*S**NS LSKVTCYISLLVPSSKAEATLEHG*PFL*PWLG VLEVAA*DFLLGSLGPSLYRHATTGTSFLPAGQ ALPFSGSSLKIPIAPDLEDITFSSHHLIKTTSSL TEHTTEGAPGEESTYCVPISRF*FSFVLTPTV* SAWYNLHFKTKEAASEWSSDTPHAG*VQSLCSS *FLV*LSSRPDLACLAQGQRQNGGV*PPPDIQAT HSVPNMYFPKCLLCASSCNRCGDTAVSNQYRQG SALMELTLQWQTNRP*IRKR*NKIYTR*V*LPF *PPLLCPPLLRSQDQRPNSSDLRADSPPLQL*DP FLRTSVGSRERGQRGKMRG*GKVLHDPHFGLSI SQEELWPRNNRGN*SSV*QSLGLSLLSYGPETP VSP*LAGNCSHHSFWPLLNAGNAILPCSCSCSDV ERSFGCWKSP*RACVLSFLADSISPLLLIPLTL LENPLLSY*FQSWWFYQGCILAKGWACESILGN QTPSP*KLARGD*DH*L*LIPTAGSDKMVHLFLL PRSPGQPGSLPFSRL*LFLPSGGLFQEFLFCLN QLEFVSVACNQAFMAAGLSDTSTLMAWRDF*SV KMQSDKCFGKSTLEKGG*QGRAGRTGSWQNGGGC SRGPTRS*WQLGLGEGL*GWKDGMGSRGIPLLN ASA*IR*TLLSIDNCT*KFCKGKQAKCTKKASS E*RILMLVI*TFVPGVLIWWM*WLVLFVSLVGL* HTVI*SNSNLGVAVKVFCRCD*HLQSVDFK*KR LLK*FG*AAPD*LKGLKNKHCSFLEKKKLCLKT IAIDSCLSFQPASLPYGFEVCQPQQLCELISKNK AIYSHPLVFVGDLFQDLYRYQNLWFLKSFI*SG IVFAYNLCAISCITSIISRLLIIPNTV*MLVVI L

Frame 1

GDSWREERGWSRGKGGEGMGWSSGQCARRTHRS LHSASRTPAAAP*AGVSCSGSCCSCSCSRRSPS CSRTQGRPRQGLFGRKPQAPREMSSLSPASNRLG THPDSHCQWRRSPLVKTSGEHGRWKYI*EPGCF IWGLRDPH*ANEETEAHESLLLLSMPAPGHLCP LRP*PLPV*LHPHGLFRPQLHHPWPVDLAPEFTA AQPLFHPLPAHSRALVLGVCQCHLHPRDAHAPG THSALQPYPQSPHLQLST*LHQLGVFLQRELLH SYSALCA*RLPHTHGNQREEAVARCPAPGPPLPA QEEVHT*PPRHQPHVCLLCTTLHPPVLQNFWQD GSWLHQGLGPWGRPRPHLWRQSGASVSTAAL*G WETQVPGAGWRNVPALGRRGACVDALPPRHPAPE PDGCGPGGVWAASWAHAVCHALAT*AQPCV*PA EG*APHLGR*AAFPDDPPHPHRGDHQDCHRGVR AAAEWLFPAAEI*PRAAVRCPVPIPQPHCHGVQP SLPLAPPHA*LLQGGLPGVQLRAVLVQHLHVGG LWG*GPGGCLLSPDCWPDRWGQEHGPPHPACGC GCHQGVSGDAAAALQ*VPQEVWHETLHLLPGARR REGDGSRVGGIVWRH*CVGVLPWTAS*KVPSKL YLWGEYDRDWGSLFPQGSPRESHLFSGVLEAEH IWRRGGL*HCQDGHTEEAGLPQHQDLSLRFLPCA GCQSG*WACCGATIHRALRGRKAAFWRGELCAC HSRVLRPGLMVLNAHFLVWHGECWG*HLEL*VS PIIWNIVILFILPEC*TPC*PFRLLGVVLIWSAR ILGS*LTT*NVRFLVDL*HSHSRMWSY**NLLE S*GVLILHSRILTF*LVIQSVVFLADDPEQWLV SQICQHLAV*NVDLIHFPVQ*DIIETEILRSNKN AFPESVPALRGQGSGVFFLGPPLRPWSEDVERT GGLYSRHWLEATRALSPSRS*LMAAVSHEANKI RFLKLPVIYLFWSHPLKQRQHWNMASLSCSHGWA C*RLQHETFCWDPWAHHCIDMLPLVLPFSLRAR HCPFQEALLKYPLPQTWKI*HSVPTI*LKQLPP LQSIQQRGHPGRRAHTVFQFHAFNSHLFSHQQCE VRGIISISKPRKQPQSGRVTHLTQAESRACAPL DSWFDSVPGLILPVWLRVKDRMVECSLHLIFRL LIQSQICIFLSVYYVPVPVTGVGTQQ*VINTDKV LPLWSSHSSGRQTDHK*GNDEIRYIQGECDFPS NPLCSVLPYCALKTRDPTAVISGQTALHSSSET LFSGPL*AAEREDRGVR*GVEGRFFMIHTLGLVF LRKSYGPETTGETRVRSDSPWG*VSCLMVQKLL FLLSWLETAPIIPSGLC*MQGMQSFPALAVALT *KDPSGAGSLHEELVSCPFLQILFPLFC*YLLLC LRILSFLINFSLGGSIRGAFWPRGGPVNQSWAI RHPLLKNWPVETEITDSDSSPQLALTRWSICSC FRDPQGSLDPCPSQDFSFSFHPVAYSRNSSFA*I SWSLCLLLVIKPLWLLG*VTQAL*WPGGTFNQ* RCNQTSVLERAPSRRVDDRAEQEGQEAGRTEEA AAVVQPGADGSWG*GKGFEGGRMGWVPEVFLS*M QVPRLGRLCLVLTTAHESFAKGNRLNAPRKLLQ SEES*CL*FKHLFLEF*FGGCDGWFYLSVWLGY STQLFNQTVI*VWL*RYFVDVINIYNQLTLSERD YLNNLGELHLIS*KASRTNTAVSWKRRNFASRL *PSTPA*VSSLLVCPMDLKFANPNNCVN*FLKI KLYTAILWYLWGICFRISTDTKICGSSSPLYEVV *YLHITYVQSPV*LQSSLDYL*YLIQCKC*LLY C

Frame 2

VTAGGRSGGGAGGRVGRGWAGAPGSVRGARTGA CTLRPAPQQPRHEPESLALVLAVPAPAPAAPRP ARGPRGAHARASLGGSRRHQGK*VPFLQPLTVW EPILIPIASGEGLPW*RLRENMGDGNTFRSRDAS SGV*EIPIEQMRKPRLMNPCCYYPCQHQGICVR FGLDRYQCDCTRTGYSGPNCTIPGLWTWLRNSL RPSPSFTHFLLTHGRWFWEFVNATFIREMLMRLV LTVRSNLIPSPPTYNSAHDYISWESFSNVSYYT RILPSVPKDCPTPMGTKGKKQLPDAQLLARRFL LRRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKM GPGFTKALGHGVDLGHIYGDNLERQYQLRLFKD GKLKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQ SQMAVGQEVFGLLPGLMLYATLWLREHNRVCDLL KAEHPTWGDEQLFQTTRLILIGETIKIVIEEYV QQLSGYFLQLKFDPELLFGVQFQYRNRIAMEFN HLYHWHPLMPDSFKVGSQEYSYEQFLFNTSMLVD YGVEALVDAFSRQIAGRIGGGRNMDHHILHVAV DVIRESREMRLQPFNEYRKRFGMKPYTSFQELV GEKEMAAELEELYGDIDALEFYPGLLLEKCHPNS IFGESMIEIGAPFSLKGLLGNPICSPEYWKPST FGGEVGFNIVKTATLKKLVCLNTKTCPYVSFRV PDASQDDGPAVERPSTEL*GAGKQHSGGESFVLV IPEC*GQG*WS*MLIFWFGMVSVGVDI*NFKSH PLSGIL*FCLFFQNAELLVSPSDC*EWFSFGLP EYWVLS*QPRMSDFWLICNTVILGCGATDEIC*K VRGFLFCIPES*LSDW*FKVLCSWLMIQNSGSY PKSVSIWLSRMWI*FIFLFSEIS*RRRS*GPTR MHSLNLCLH*EGKEVGCSSWDPH*DPGLRM*REQ VGCIHAIGWKLPELYPHPGLDSWQLFLMKLIKF AF*SYLLYISFGPIL*SRGNTGTWLAFLVAMAG RARGCSMRLSAGILGPITV*TCYHWYFLSPCGPG TALFRKLS*NTHCPRPGRYNIQFPPSD*NNFLP YRAYNRGGTRGGEHILCSNFTLLILICSHTNSV KCVV*SPFQNQGSSLRVVE*HTSRRLSPELVLLL IPGLTQFQA*SCLSGSGSKTEWWSVAST*YSGY SFSPKYVFS*VFTMCQFL*QVWGHSSE*SIQTR FCPYGAHTPVADKQTINKETMK*DIYKVSVTSLL TPSALSSPIALSRPETQQQ*SQGRQPSTPALRP FSQDLCRQQRERTEG*DEGLREGSS*STLWA*Y FSGRAMAQKQQGKLEFGLTVLGVKSPVLWSRNSC FSLVGWKLLPSFLLASAECRECNPSLLLQLL*R RKILRVLEVSMKSLCPVLSCRFYFPSSANTSYF A*ESSPFLLISVLVVLSGVHSGQGVGL*INPGQS DTLSLKTGPWRLRSLTLTHPHSWL*QDGPFVPA SEIPRAAWIPALLKTLAFPSIRWPIPGIPLLLK SVGVCVCCL*SSLYGCWAE*HKHFNGLEGLLISE DAIRQVFWKEHPREGWMTGQSRKDRKLAERRRL QPWSNQELMAAGARGRALRVEGWDGFQRYSSLK CKCLD*VDFA*Y*QLHMKVLQRETG*MHQESFFR VKNLNACNLNICSWSFDLVDVMVGFICQFGWAI AHSYLIKQ*SRCGCEGIL*M*LTSTIS*L*VKE IT*IIWVSCT*LVERPQEQTLQFPGKEETLPQDY SHRLLPEFPAC*SALWI*SLPTPTIV*INF*K* SYIQPSFGICGGFVSGSLQIPKSVVPQVLYMKW YSICI*PMCNLLYNFNHL*ITYNT*YSVNASCYT

Sequence PTGS1.aAug05

AGGTGACAGCTGGAGGGAGGAGCGGGGGTGGAGCCGGGGGAAGGGTGGGGAGGGGATGGGCTGGAGCTCCGGGCAGTGTGCGAGGCGCACGCACAGGAGC CTGCACTCTGCGTCCCGCACCCCAGCAGCCGCGCCATGAGCCGGAGTCTCTTGCTCTGGTTCTTGCTGTTCCTGCTCCTGCTCCCGCCGCTCCCCGTCCT GCTCGCGGACCCAGGGGCGCCCACGCCAGTGAATCCCTGTTGTTACTATCCATGCCAGCACCAGGGCATCTGTGTCCGCTTCGGCCTTGACCGCTACCAG TGTGACTGCACCCGCACGGGCTATTCCGGCCCCAACTGCACCATCCCTGGCCTGTGGACCTGGCTCCGGAATTCACTGCGGCCCAGCCCCTCTTTCACCC ACTTCCTGCTCACTCACGGGCGCTGGTTCTGGGAGTTTGTCAATGCCACCTTCATCCGAGAGATGCTCATGCGCCTGGTACTCACAGTGCGCTCCAACCT TATCCCCAGTCCCCCCACCTACAACTCAGCACATGACTACATCAGCTGGGAGTCTTTCTCCAACGTGAGCTATTACACTCGTATTCTGCCCTCTGTGCCT AAAGATTGCCCCACACCCATGGGAACCAAAGGGAAGAAGCAGTTGCCAGATGCCCAGCTCCTGGCCCGCCGCTTCCTGCTCAGGAGGAAGTTCATACCTG ACCCCCAAGGCACCAACCTCATGTTTGCCTTCTTTGCACAACACTTCACCCACCAGTTCTTCAAAACTTCTGGCAAGATGGGTCCTGGCTTCACCAAGGC CTTGGGCCATGGGGTAGACCTCGGCCACATTTATGGAGACAATCTGGAGCGTCAGTATCAACTGCGGCTCTTTAAGGATGGGAAACTCAAGTACCAGGTG CTGGATGGAGAAATGTACCCGCCCTCGGTAGAAGAGGCGCCTGTGTTGATGCACTACCCCCGAGGCATCCCGCCCCAGAGCCAGATGGCTGTGGGCCAGG AGGTGTTTGGGCTGCTTCCTGGGCTCATGCTGTATGCCACGCTCTGGCTACGTGAGCACAACCGTGTGTGTGACCTGCTGAAGGCTGAGCACCCCACCTG GGGCGATGAGCAGCTTTTCCAGACGACCCGCCTCATCCTCATAGGGGAGACCATCAAGATTGTCATCGAGGAGTACGTGCAGCAGCTGAGTGGCTATTTC CTGCAGCTGAAATTTGACCCAGAGCTGCTGTTCGGTGTCCAGTTCCAATACCGCAACCGCATTGCCATGGAGTTCAACCATCTCTACCACTGGCACCCCC TCATGCCTGACTCCTTCAAGGTGGGCTCCCAGGAGTACAGCTACGAGCAGTTCTTGTTCAACACCTCCATGTTGGTGGACTATGGGGTTGAGGCCCTGGT GGATGCCTTCTCTCGCCAGATTGCTGGCCGGATCGGTGGGGGCAGGAACATGGACCACCACATCCTGCATGTGGCTGTGGATGTCATCAGGGAGTCTCGG GAGATGCGGCTGCAGCCCTTCAATGAGTACCGCAAGAGGTTTGGCATGAAACCCTACACCTCCTTCCAGGAGCTCGTAGGAGAGAAGGAGATGGCAGCAG AGTTGGAGGAATTGTATGGAGACATTGATGCGTTGGAGTTCTACCCTGGACTGCTTCTTGAAAAGTGCCATCCAAACTCTATCTTTGGGGAGAGTATGAT AGAGATTGGGGCTCCCTTTTCCCTCAAGGGTCTCCTAGGGAATCCCATCTGTTCTCCGGAGTACTGGAAGCCGAGCACATTTGGCGGCGAGGTGGGCTTT AACATTGTCAAGACGGCCACACTGAAGAAGCTGGTCTGCCTCAACACCAAGACCTGTCCCTACGTTTCCTTCCGTGTGCCGGATGCCAGTCAGGATGATG GGCCTGCTGTGGAGCGACCATCCACAGAGCTCTGAGGGGCAGGAAAGCAGCATTCTGGAGGGGAGAGCTTTGTGCTTGTCATTCCAGAGTGCTGAGGCCA GGGCTGATGGTCTTAAATGCTCATTTTCTGGTTTGGCATGGTGAGTGTTGGGGTTGACATTTAGAACTTTAAGTCTCACCCATTATCTGGAATATTGTGA TTCTGTTTATTCTTCCAGAATGCTGAACTCCTTGTTAGCCCTTCAGATTGTTAGGAGTGGTTCTCATTTGGTCTGCCAGAATACTGGGTTCTTAGTTGAC AACCTAGAATGTCAGATTTCTGGTTGATTTGTAACACAGTCATTCTAGGATGTGGAGCTACTGATGAAATCTGCTAGAAAGTTAGGGGGTTCTTATTTTG CATTCCAGAATCTTGACTTTCTGATTGGTGATTCAAAGTGTTGTGTTCCTGGCTGATGATCCAGAACAGTGGCTCGTATCCCAAATCTGTCAGCATCTGG CTGTCTAGAATGTGGATTTGATTCATTTTCCTGTTCAGTGAGATATCATAGAGACGGAGATCCTAAGGTCCAACAAGAATGCATTCCCTGAATCTGTGCC TGCACTGAGAGGGCAAGGAAGTGGGGTGTTCTTCTTGGGACCCCCACTAAGACCCTGGTCTGAGGATGTAGAGAGAACAGGTGGGCTGTATTCACGCCAT TGGTTGGAAGCTACCAGAGCTCTATCCCCATCCAGGTCTTGACTCATGGCAGCTGTTTCTCATGAAGCTAATAAAATTCGCTTTCTAAAGTTACCTGTTA TATATCTCTTTTGGTCCCATCCTCTAAAGCAGAGGCAACACTGGAACATGGCTAGCCTTTCTTGTAGCCATGGCTGGGCGTGCTAGAGGTTGCAGCATGA GACTTTCTGCTGGGATCCTTGGGCCCATCACTGTATAGACATGCTACCACTGGTACTTCCTTTCTCCCTGCGGGCCAGGCACTGCCCTTTTCAGGAAGCT CTCTTAAAATACCCATTGCCCCAGACCTGGAAGATATAACATTCAGTTCCCACCATCTGATTAAAACAACTTCCTCCCTTACAGAGCATACAACAGAGGG GGCACCCGGGGAGGAGAGCACATACTGTGTTCCAATTTCACGCTTTTAATTCTCATTTGTTCTCACACCAACAGTGTGAAGTGCGTGGTATAATCTCCAT TTCAAAACCAAGGAAGCAGCCTCAGAGTGGTCGAGTGACACACCTCACGCAGGCTGAGTCCAGAGCTTGTGCTCCTCTTGATTCCTGGTTTGACTCAGTT CCAGGCCTGATCTTGCCTGTCTGGCTCAGGGTCAAAGACAGAATGGTGGAGTGTAGCCTCCACCTGATATTCAGGCTACTCATTCAGTCCCAAATATGTA TTTTCCTAAGTGTTTACTATGTGCCAGTTCCTGTAACAGGTGTGGGGACACAGCAGTGAGTAATCAATACAGACAAGGTTCTGCCCTTATGGAGCTCACA CTCCAGTGGCAGACAAACAGACCATAAATAAGGAAACGATGAAATAAGATATATACAAGGTGAGTGTGACTTCCCTTCTAACCCCCTCTGCTCTGTCCTC CCCTATTGCGCTCTCAAGACCAGAGACCCAACAGCAGTGATCTCAGGGCAGACAGCCCTCCACTCCAGCTCTGAGACCCTTTTCTCAGGACCTCTGTAGG CAGCAGAGAGAGAGGACAGAGGGGTAAGATGAGGGGTTGAGGGAAGGTTCTTCATGATCCACACTTTGGGCTTAGTATTTCTCAGGAAGAGCTATGGCCC AGAAACAACAGGGGAAACTAGAGTTCGGTCTGACAGTCCTTGGGGTTAAGTCTCCTGTCTTATGGTCCAGAAACTCCTGTTTCTCCTTAGTTGGCTGGAA ACTGCTCCCATCATTCCTTCTGGCCTCTGCTGAATGCAGGGAATGCAATCCTTCCCTGCTCTTGCAGTTGCTCTGACGTAGAAAGATCCTTCGGGTGCTG GAAGTCTCCATGAAGAGCTTGTGTCCTGTCCTTTCTTGCAGATTCTATTTCCCCTCTTCTGCTAATACCTCTTACTTTGCTTGAGAATCCTCTCCTTTCT TATTAATTTCAGTCTTGGTGGTTCTATCAGGGGTGCATTCTGGCCAAGGGGTGGGCCTGTGAATCAATCCTGGGCAATCAGACACCCTCTCCTTAAAAAC TGGCCCGTGGAGACTGAGATCACTGACTCTGACTCATCCCCACAGCTGGCTCTGACAAGATGGTCCATTTGTTCCTGCTTCCGAGATCCCCAGGGCAGCC TGGATCCCTGCCCTTCTCAAGACTTTAGCTTTTCCTTCCATCCGGTGGCCTATTCCAGGAATTCCTCTTTTGCTTAAATCAGTTGGAGTTTGTGTCTGTT GCTTGTAATCAAGCCTTTATGGCTGCTGGGCTGAGTGACACAAGCACTTTAATGGCCTGGAGGGACTTTTAATCAGTGAAGATGCAATCAGACAAGTGTT TTGGAAAGAGCACCCTCGAGAAGGGTGGATGACAGGGCAGAGCAGGAAGGACAGGAAGCTGGCAGAACGGAGGAGGCTGCAGCCGTGGTCCAACCAGGAG CTGATGGCAGCTGGGGCTAGGGGAAGGGCTTTGAGGGTGGAAGGATGGGATGGGTTCCAGAGGTATTCCTCTCTTAAATGCAAGTGCCTAGATTAGGTAG ACTTTGCTTAGTATTGACAACTGCACATGAAAGTTTTGCAAAGGGAAACAGGCTAAATGCACCAAGAAAGCTTCTTCAGAGTGAAGAATCTTAATGCTTG TAATTTAAACATTTGTTCCTGGAGTTTTGATTTGGTGGATGTGATGGTTGGTTTTATTTGTCAGTTTGGTTGGGCTATAGCACACAGTTATTTAATCAAA CAGTAATCTAGGTGTGGCTGTGAAGGTATTTTGTAGATGTGATTAACATCTACAATCAGTTGACTTTAAGTGAAAGAGATTACTTAAATAATTTGGGTGA GCTGCACCTGATTAGTTGAAAGGCCTCAAGAACAAACACTGCAGTTTCCTGGAAAAGAAGAAACTTTGCCTCAAGACTATAGCCATCGACTCCTGCCTGA GTTTCCAGCCTGCTAGTCTGCCCTATGGATTTGAAGTTTGCCAACCCCAACAATTGTGTGAATTAATTTCTAAAAATAAAGCTATATACAGCCATCCTTT GGTATTTGTGGGGGATTTGTTTCAGGATCTCTACAGATACCAAAATCTGTGGTTCCTCAAGTCCTTTATATGAAGTGGTATAGTATTTGCATATAACCTA TGTGCAATCTCCTGTATAACTTCAATCATCTCTAGATTACTTATAATACCTAATACAGTGTAAATGCTAGTTGTTATACTGT

PTGS1.u.f.15 (forward primer)
PTGS1.u.r.17 (reverse primer)
Alternative donor

Frame 0

R*QLEGGAGVEPGEGWGGDGLELRAVCEAHAQE PALCVPHPSSRAMSRSLLLWFLLFLLLLPPLPVL LADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQ CDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFT HFLLTHGRWFWEFVNATFIREMLMRLVLTVRSNL IPSPPTYNSAHDYISWESFSNVSYYTRILPSVP KDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIP DPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKA LGHGVDLGHIYGDNLERQYQLRLFKDGKLKYQV LDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQ EVFGLLPGLMLYATLWLREHNRVCDLLKAEHPTW GDEQLFQTTRLILIGETIKIVIEEYVQQLSGYF LQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHP LMPDSFKVGSQEYSYEQFLFNTSMLVDYGVEALV DAFSRQIAGRIGGGRNMDHHILHVAVDVIRESR EMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAA ELEELYGDIDALEFYPGLLLEKCHPNSIFGESMI EIGAPFSLKGLLGNPICSPEYWKPSTFGGEVGF NIVKTATLKKLVCLNTKTCPYVSFRVPDASQDD GPAVERPSTEL*GAGKQHSGGESFVLVIPEC*GQ G*WS*MLIFWFGMVSVGVDI*NFKSHPLSGIL* FCLFFQNAELLVSPSDC*EWFSFGLPEYWVLS* QPRMSDFWLICNTVILGCGATDEIC*KVRGFLFC IPES*LSDW*FKVLCSWLMIQNSGSYPKSVSIW LSRMWI*FIFLFSEIS*RRRS*GPTRMHSLNLC LH*EGKEVGCSSWDPH*DPGLRM*REQVGCIHAI GWKLPELYPHPGLDSWQLFLMKLIKFAF*SYLL YISFGPIL*SRGNTGTWLAFLVAMAGRARGCSM RLSAGILGPITV*TCYHWYFLSPCGPGTALFRKL S*NTHCPRPGRYNIQFPPSD*NNFLPYRAYNRG GTRGGEHILCSNFTLLILICSHTNSVKCVV*SP FQNQGSSLRVVE*HTSRRLSPELVLLLIPGLTQF QA*SCLSGSGSKTEWWSVAST*YSGYSFSPKYV FS*VFTMCQFL*QVWGHSSE*SIQTRFCPYGAH TPVADKQTINKETMK*DIYKVSVTSLLTPSALSS PIALSRPETQQQ*SQGRQPSTPALRPFSQDLCR QQRERTEG*DEGLREGSS*STLWA*YFSGRAMA QKQQGKLEFGLTVLGVKSPVLWSRNSCFSLVGWK LLPSFLLASAECRECNPSLLLQLL*RRKILRVL EVSMKSLCPVLSCRFYFPSSANTSYFA*ESSPF LLISVLVVLSGVHSGQGVGL*INPGQSDTLSLKT GPWRLRSLTLTHPHSWL*QDGPFVPASEIPRAA WIPALLKTLAFPSIRWPIPGIPLLLKSVGVCVC CL*SSLYGCWAE*HKHFNGLEGLLISEDAIRQVF WKEHPREGWMTGQSRKDRKLAERRRLQPWSNQE LMAAGARGRALRVEGWDGFQRYSSLKCKCLD*V DFA*Y*QLHMKVLQRETG*MHQESFFRVKNLNAC NLNICSWSFDLVDVMVGFICQFGWAIAHSYLIK Q*SRCGCEGIL*M*LTSTIS*L*VKEIT*IIWV SCT*LVERPQEQTLQFPGKEETLPQDYSHRLLPE FPAC*SALWI*SLPTPTIV*INF*K*SYIQPSF GICGGFVSGSLQIPKSVVPQVLYMKWYSICI*P MCNLLYNFNHL*ITYNT*YSVNASCYT

Frame 1

GDSWREERGWSRGKGGEGMGWSSGQCARRTHRS LHSASRTPAAAP*AGVSCSGSCCSCSCSRRSPS CSRTQGRPRQ*IPVVTIHASTRASVSASALTATS VTAPARAIPAPTAPSLACGPGSGIHCGPAPLSP TSCSLTGAGSGSLSMPPSSERCSCAWYSQCAPT LSPVPPPTTQHMTTSAGSLSPT*AITLVFCPLCL KIAPHPWEPKGRSSCQMPSSWPAASCSGGSSYL TPKAPTSCLPSLHNTSPTSSSKLLARWVLASPR PWAMG*TSATFMETIWSVSINCGSLRMGNSSTRC WMEKCTRPR*KRRLC*CTTPEASRPRARWLWAR RCLGCFLGSCCMPRSGYVSTTVCVTC*RLSTPP GAMSSFSRRPASSS*GRPSRLSSRSTCSS*VAIS CS*NLTQSCCSVSSSNTATALPWSSTISTTGTP SCLTPSRWAPRSTATSSSCSTPPCWWTMGLRPW WMPSLARLLAGSVGAGTWTTTSCMWLWMSSGSLG RCGCSPSMSTARGLA*NPTPPSRSS*ERRRWQQ SWRNCMETLMRWSSTLDCFLKSAIQTLSLGRV* *RLGLPFPSRVS*GIPSVLRSTGSRAHLAARWAL TLSRRPH*RSWSASTPRPVPTFPSVCRMPVRMM GLLWSDHPQSSEGQESSILEGRALCLSFQSAEA RADGLKCSFSGLAW*VLGLTFRTLSLTHYLEYCD SVYSSRMLNSLLALQIVRSGSHLVCQNTGFLVD NLECQISG*FVTQSF*DVELLMKSARKLGGSYF AFQNLDFLIGDSKCCVPG**SRTVARIPNLSASG CLECGFDSFSCSVRYHRDGDPKVQQECIP*ICA CTERARKWGVLLGTPTKTLV*GCRENRWAVFTP LVGSYQSSIPIQVLTHGSCFS*S**NSLSKVTCY ISLLVPSSKAEATLEHG*PFL*PWLGVLEVAA* DFLLGSLGPSLYRHATTGTSFLPAGQALPFSGS SLKIPIAPDLEDITFSSHHLIKTTSSLTEHTTEG APGEESTYCVPISRF*FSFVLTPTV*SAWYNLH FKTKEAASEWSSDTPHAG*VQSLCSS*FLV*LS SRPDLACLAQGQRQNGGV*PPPDIQATHSVPNMY FPKCLLCASSCNRCGDTAVSNQYRQGSALMELT LQWQTNRP*IRKR*NKIYTR*V*LPF*PPLLCP PLLRSQDQRPNSSDLRADSPPLQL*DPFLRTSVG SRERGQRGKMRG*GKVLHDPHFGLSISQEELWP RNNRGN*SSV*QSLGLSLLSYGPETPVSP*LAG NCSHHSFWPLLNAGNAILPCSCSCSDVERSFGCW KSP*RACVLSFLADSISPLLLIPLTLLENPLLS Y*FQSWWFYQGCILAKGWACESILGNQTPSP*K LARGD*DH*L*LIPTAGSDKMVHLFLLPRSPGQP GSLPFSRL*LFLPSGGLFQEFLFCLNQLEFVSV ACNQAFMAAGLSDTSTLMAWRDF*SVKMQSDKC FGKSTLEKGG*QGRAGRTGSWQNGGGCSRGPTRS *WQLGLGEGL*GWKDGMGSRGIPLLNASA*IR* TLLSIDNCT*KFCKGKQAKCTKKASSE*RILML VI*TFVPGVLIWWM*WLVLFVSLVGL*HTVI*SN SNLGVAVKVFCRCD*HLQSVDFK*KRLLK*FG* AAPD*LKGLKNKHCSFLEKKKLCLKTIAIDSCL SFQPASLPYGFEVCQPQQLCELISKNKAIYSHPL VFVGDLFQDLYRYQNLWFLKSFI*SGIVFAYNL CAISCITSIISRLLIIPNTV*MLVVIL

Frame 2

VTAGGRSGGGAGGRVGRGWAGAPGSVRGARTGA CTLRPAPQQPRHEPESLALVLAVPAPAPAAPRP ARGPRGAHASESLLLLSMPAPGHLCPLRP*PLP V*LHPHGLFRPQLHHPWPVDLAPEFTAAQPLFHP LPAHSRALVLGVCQCHLHPRDAHAPGTHSALQP YPQSPHLQLST*LHQLGVFLQRELLHSYSALCA *RLPHTHGNQREEAVARCPAPGPPLPAQEEVHT* PPRHQPHVCLLCTTLHPPVLQNFWQDGSWLHQG LGPWGRPRPHLWRQSGASVSTAAL*GWETQVPG AGWRNVPALGRRGACVDALPPRHPAPEPDGCGPG GVWAASWAHAVCHALAT*AQPCV*PAEG*APHL GR*AAFPDDPPHPHRGDHQDCHRGVRAAAEWLF PAAEI*PRAAVRCPVPIPQPHCHGVQPSLPLAPP HA*LLQGGLPGVQLRAVLVQHLHVGGLWG*GPG GCLLSPDCWPDRWGQEHGPPHPACGCGCHQGVS GDAAAALQ*VPQEVWHETLHLLPGARRREGDGSR VGGIVWRH*CVGVLPWTAS*KVPSKLYLWGEYD RDWGSLFPQGSPRESHLFSGVLEAEHIWRRGGL *HCQDGHTEEAGLPQHQDLSLRFLPCAGCQSG*W ACCGATIHRALRGRKAAFWRGELCACHSRVLRP GLMVLNAHFLVWHGECWG*HLEL*VSPIIWNIV ILFILPEC*TPC*PFRLLGVVLIWSARILGS*LT T*NVRFLVDL*HSHSRMWSY**NLLES*GVLIL HSRILTF*LVIQSVVFLADDPEQWLVSQICQHL AV*NVDLIHFPVQ*DIIETEILRSNKNAFPESVP ALRGQGSGVFFLGPPLRPWSEDVERTGGLYSRH WLEATRALSPSRS*LMAAVSHEANKIRFLKLPV IYLFWSHPLKQRQHWNMASLSCSHGWAC*RLQHE TFCWDPWAHHCIDMLPLVLPFSLRARHCPFQEA LLKYPLPQTWKI*HSVPTI*LKQLPPLQSIQQR GHPGRRAHTVFQFHAFNSHLFSHQQCEVRGIISI SKPRKQPQSGRVTHLTQAESRACAPLDSWFDSV PGLILPVWLRVKDRMVECSLHLIFRLLIQSQIC IFLSVYYVPVPVTGVGTQQ*VINTDKVLPLWSSH SSGRQTDHK*GNDEIRYIQGECDFPSNPLCSVL PYCALKTRDPTAVISGQTALHSSSETLFSGPL* AAEREDRGVR*GVEGRFFMIHTLGLVFLRKSYGP ETTGETRVRSDSPWG*VSCLMVQKLLFLLSWLE TAPIIPSGLC*MQGMQSFPALAVALT*KDPSGA GSLHEELVSCPFLQILFPLFC*YLLLCLRILSFL INFSLGGSIRGAFWPRGGPVNQSWAIRHPLLKN WPVETEITDSDSSPQLALTRWSICSCFRDPQGS LDPCPSQDFSFSFHPVAYSRNSSFA*ISWSLCLL LVIKPLWLLG*VTQAL*WPGGTFNQ*RCNQTSV LERAPSRRVDDRAEQEGQEAGRTEEAAAVVQPG ADGSWG*GKGFEGGRMGWVPEVFLS*MQVPRLGR LCLVLTTAHESFAKGNRLNAPRKLLQSEES*CL *FKHLFLEF*FGGCDGWFYLSVWLGYSTQLFNQ TVI*VWL*RYFVDVINIYNQLTLSERDYLNNLGE LHLIS*KASRTNTAVSWKRRNFASRL*PSTPA* VSSLLVCPMDLKFANPNNCVN*FLKIKLYTAIL WYLWGICFRISTDTKICGSSSPLYEVV*YLHITY VQSPV*LQSSLDYL*YLIQCKC*LLYC