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Sequences

Sequence PPP3CC.iAug05

CAGTTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTTACTATCAATTATCAGAGCCCATGAAGCCCAAGATGCTGGGTAAGTTACATTAAAATTG
TACCAAATATCGCTGCAGAGTCTTTGCATTTAATATGCAGACAGATGGACTTTCATCTCTTTTTCAGGTATCGAATGTACAGGAAGAGCCAAGCCACAGG
CTTTCCATCACTTATTACAATTTTCTCTGCCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATATC
AGGCAGTTTAACTGTTCTCCACACCCCTACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTGCCTTTTGTTGGGGAAAAAGTCACAGAGATGC
TGGTAAATGTGCTCAACATATGCTCTGATGACGAACTGATTTCTGATGATGAAGCAGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGA
GCCATTGGGAAGATGGCACGGGTCTTTTCAATTCTTCGGCAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGG
GCGTCCTCTCAGGAGGCAAGCAGACTATCGAGACAGCCACAGTAGAAGCGGTAGAGGCCCGGGAAGCTTTAATGCAACGGCAGAAGCTTAAGCAACTGGT
CATGAGAGGTCAAGTGGTTTACTTCTGTATCCCTTCCATGTACAAGAGACATCCATTTGATTCTCAAGAGAGCCAAATAGGTCAGCCTCTTCAGCGATTC
TAAAAGATTTCAAGAGCAGAGGCAGAAGTAGACTGGAATTTAGTTCAATTCATTTCTGAGGTTGCCCTAAGGTAGGCAAGTTAAATTAACTTTGTTTCTA
TGAAAACCATTGGAACTGATAAACTTGTATTATACTTGTAATTGTTTTATTAGGTAAGATGGTTCAAAACGCGAATGCCTTAACTTGGTGTTATAAAAGT
TGAATCGGAAGGAAAATATGTAGAAGCTATTTTTAAGTTTTTAACTTATGTACAGCTCCTGGTTGTGCAAGATAGAAAGCTGTCCTAAACGAAAAGATTA
GCTCTTTTGTTACTTTTTTTTGTGGTCTATGTGGCCTTTGTGCG
  • PPP3CC.u.f.15 (forward primer)
  • PPP3CC.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

QLPCSL*IFAEQ*FTINYQSP*SPRCWVSYIKI
VPNIAAESLHLICRQMDFHLFFRYRMYRKSQATG
FPSLITIFSAPNYLDVYNNKAAVLKYENNVMNI
RQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEM
LVNVLNICSDDELISDDEAEALQFVRRSSGIRSE
PLGRWHGSFQFFGKKVRVC*LSRA*LPQAHSLW
ASSQEASRLSRQPQ*KR*RPGKL*CNGRSLSNW
S*EVKWFTSVSLPCTRDIHLILKRAK*VSLFSDS
KRFQEQRQK*TGI*FNSFLRLP*GRQVKLTLFL
*KPLELINLYYTCNCFIR*DGSKRECLNLVL*K
LNRKENM*KLFLSF*LMYSSWLCKIESCPKRKD*
LFCYFFLWSMWPLC

Frame 1

SYPAVCEFLQNNNLLSIIRAHEAQDAG*VTLKL
YQISLQSLCI*YADRWTFISFSGIECTGRAKPQ
AFHHLLQFSLPPIT*MSITIKLLC*NMKTMS*IS
GSLTVLHTPTGFQTLWMFSHGLCLLLGKKSQRC
W*MCSTYALMTN*FLMMKQKHYSS*GDHQE*DQ
SHWEDGTGLFNSSARK*ECADSQGPDSHRHTPSG
RPLRRQADYRDSHSRSGRGPGSFNATAEA*ATG
HERSSGLLLYPFHVQETSI*FSREPNRSASSAI
LKDFKSRGRSRLEFSSIHF*GCPKVGKLN*LCFY
ENHWN**TCIILVIVLLGKMVQNANALTWCYKS
*IGRKICRSYF*VFNLCTAPGCAR*KAVLNEKI
SSFVTFFCGLCGLCA

Frame 2

VTLQFVNFCRTIIYYQLSEPMKPKMLGKLH*NC
TKYRCRVFAFNMQTDGLSSLFQVSNVQEEPSHR
LSITYYNFLCPQLPRCL*Q*SCCVEI*KQCHEY
QAV*LFSTPLLASKLYGCFHMVFAFCWGKSHRDA
GKCAQHML**RTDF***SRSTTVRKEIIRNKIR
AIGKMARVFSILRQESESVLTLKGLTPTGTLPL
GVLSGGKQTIETATVEAVEAREALMQRQKLKQLV
MRGQVVYFCIPSMYKRHPFDSQESQIGQPLQRF
*KISRAEAEVDWNLVQFISEVALR*AS*INFVS
MKTIGTDKLVLYL*LFY*VRWFKTRMP*LGVIKV
ESEGKYVEAIFKFLTYVQLLVVQDRKLS*TKRL
ALLLLFFVVYVAFV

Sequence PPP3CC.cAug05

TGACTATGTGGACAGAGGCTATTTCAGTATAGAGGTCGAATCAAATATTCGGAACAGGTGTATGATGCCTGTATGGAGACATTTGACTGTCTTCCTCTTG
CTGCCCTCTTAAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACTTCTTTAGATGACATTAGGAAATTAGACAGGTTTACGGAACC
TCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGGATTATGGCAATGAGAAGACCTTGGAGCACTATACCCACAACACTGTCCGA
GGGTGCTCTTATTTCTACAGTTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTTACTATCAATTATCAGAGCCCATGAAGCCCAAGATGCTGGGT
ATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCTGCCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGT
GTTGAAATATGAAAACAATGTCATGAATATCAGGCAGTTTAACTGTTCTCCACACCCCTACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTG
CCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGATGACGAACTGATTTCTGATGATGAAGCAGAAGATCACTACA
TTCCAAGCTATCAGAAAGGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGAGCCATTGGGAAGATGGCACGGGTCTTTTCAATTCTTCG
GCAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCAAGCAGACTATCGAGACAGCC
ACAGTAGAAGCGGTAGAGGCCCGGGAAGCCATCAGAGGGTTCTCGCTTCAGCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCTGGACCGAATTAATG
AGCGAATGCCACCCCGAAAGGATAGCATACACGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCTGCGCACAGGAGCGACCAAGGGAA
GAAAGCCCATTCATGACTTAGAGTCCTGCCGTGGCTCAGGTGGATCTAAAACTCAAGAACAAATTCTATTTATTTATTATTGGAAAATGAAAAGCAACTC
AAAACAACTTCAACGTGGAGGTGCATTTATAATTCAGTCTGCATTTATTCTGTAAAAAGGTGGCTGTTTTATAAATTCTTTTAATTTATGTTCAATATAT
ATAAAAAGTGCATCTGTTTTGTTTTTCCCTTTTTTCTCCATAATTTTAAGAAATGAATCTGATTGTTGTCAACACATTTGTGAAGTCTTGTGCTATAAAG
GGGAACTTCCCCTAATAAAAGGGCCTTGGAAACCTCAAACCTGGGTTTCTGACTTGAA
  • PPP3CC.u.f.15 (forward primer)
  • PPP3CC.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

*LCGQRLFQYRGRIKYSEQVYDACMETFDCLPL
AALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEP
PAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVR
GCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAG
YRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAV
LKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSL
PFVGEKVTEMLVNVLNICSDDELISDDEAEDHY
IPSYQKGSTTVRKEIIRNKIRAIGKMARVFSILR
QESESVLTLKGLTPTGTLPLGVLSGGKQTIETA
TVEAVEAREAIRGFSLQHKIRSFEEARGLDRIN
ERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGK
KAHS*LRVLPWLRWI*NSRTNSIYLLLENEKQL
KTTSTWRCIYNSVCIYSVKRWLFYKFF*FMFNI
YKKCICFVFPFFLHNFKK*I*LLSTHL*SLVL*R
GTSPNKRALETSNLGF*LE

Frame 1

DYVDRGYFSIEVESNIRNRCMMPVWRHLTVFLL
LPS*TSSFSVYMEECHLKLLL*MTLGN*TGLRN
LPPLDLCVTCFGLIPQRIMAMRRPWSTIPTTLSE
GALISTVTLQFVNFCRTIIYYQLSEPMKPKMLG
IECTGRAKPQAFHHLLQFSLPPIT*MSITIKLL
C*NMKTMS*ISGSLTVLHTPTGFQTLWMFSHGLC
LLLGKKSQRCW*MCSTYALMTN*FLMMKQKITT
FQAIRKEALQFVRRSSGIRSEPLGRWHGSFQFF
GKKVRVC*LSRA*LPQAHSLWASSQEASRLSRQP
Q*KR*RPGKPSEGSRFSTRSGVLKKREVWTELM
SECHPERIAYTLVGQ*NL*PQHTHMLRTGATKG
RKPIHDLESCRGSGGSKTQEQILFIYYWKMKSNS
KQLQRGGAFIIQSAFIL*KGGCFINSFNLCSIY
IKSASVLFFPFFSIILRNESDCCQHICEVLCYK
GELPLIKGPWKPQTWVSDL

Frame 2

TMWTEAISV*RSNQIFGTGV*CLYGDI*LSSSC
CPLKPAVSLCTWRNVT*NYFFR*H*EIRQVYGT
SRLWTCV*PALV*SLRGLWQ*EDLGALYPQHCP
RVLLFLQLPCSL*IFAEQ*FTINYQSP*SPRCWV
SNVQEEPSHRLSITYYNFLCPQLPRCL*Q*SCC
VEI*KQCHEYQAV*LFSTPLLASKLYGCFHMVF
AFCWGKSHRDAGKCAQHML**RTDF***SRRSLH
SKLSERKHYSS*GDHQE*DQSHWEDGTGLFNSS
ARK*ECADSQGPDSHRHTPSGRPLRRQADYRDS
HSRSGRGPGSHQRVLASAQDPEF*RSARSGPN**
ANATPKG*HTRWWANEICNLSTLTCCAQERPRE
ESPFMT*SPAVAQVDLKLKNKFYLFIIGK*KAT
QNNFNVEVHL*FSLHLFCKKVAVL*ILLIYVQYI
*KVHLFCFSLFSP*F*EMNLIVVNTFVKSCAIK
GNFP**KGLGNLKPGFLT*

Sequence PPP3CC.bAug05

GTTCCGGGCCCGGGTAGGGGCTGGCAGGAGAGAAGGGGCCGGCTGCGGGGAGGGCTGGCTGAGAAGAAGCGAAAATGGGCGGTTAGCAGCAGGGACCCGG
AGCCGGAGGAGCCGAGAGCAGCGCGTGCGCCGAGCTCTACTGCCTCGCGGGAAGGCGGAAGGGTGGGGAGGGCGGCGCTCGGGGCGGGAGGCCCGGCCGG
GTCCGCTAGGACAGCGGGGCCGCTGGGAAGTTGTGAGAGCGGCGCTCGGGGGCGCGCTTGCGTGCACGAGGGCCCGGGCCGCGAGCAGCCGCGGCCGTCC
CGGTCGCCACCCTTAGCAGCGGTCGCGGTCGGTGCCGAAGCGGTGTTCCCCGCCTTAGCCGCTGGCGCCTCCCAAGAGAGCGGCCGGTGGGCCCTCGTCC
TGTCAGTGGCGTCGGAGGCCGGCGCTGCGGTGGCCGCGCCCTTCTGGTGCTCGGACACCGCTGAGGAGCCGGGGCCGGGCACGGCTGGCTGACGGCTCCG
GGCAGCTAAGGCTGCCCGAGGAGAAGGCGGCGGCCGCGGCGTAGGCGCACGTCCGGCGGGCTCCTGGAGCCTGGAGGAGGCCGAGGGGACCATGTCCGGG
AGGCGCTTCCACCTCTCCACCACCGACCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCCAACGGCTTACTTTCAAGGAAGTATTTGAGAATGGGAAAC
CTAAAGTTGATGTTTTAAAAAACCATTTGGTAAAGGAAGGACGACTGGAAGAGGAAGTAGCCTTAAAGATAATCAATGATGGGGCTGCCATCCTGAGGCA
AGAGAAGACTATGATAGAAGTAGATGCTCCAATCACAGTATGTGGTGATATTCATGGACAATTCTTTGACCTAATGAAGTTATTTGAAGTTGGAGGATCA
CCTAGTAACACACGCTACCTCTTTCTGGGTGACTATGTGGACAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATTTATGGAGTTTAAAGATTAATCATC
CCAAAACATTGTTTCTGCTTCGGGGAAATCATGAATGCAGGCATCTTACAGACTATTTCACCTTCAAACAGGAATGTCGAATCAAATATTCGGAACAGGT
GTATGATGCCTGTATGGAGACATTTGACTGTCTTCCTCTTGCTGCCCTCTTAAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACT
TCTTTAGATGACATTAGGAAATTAGACAGGTTTACGGAACCTCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGGATTATGGCA
ATGAGAAGACCTTGGAGCACTATACCCACAACACTGTCCGAGGGTGCTCTTATTTCTACAGTTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTT
ACTATCAATTATCAGAGCCCATGAAGCCCAAGATGCTGGGTATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCT
GCCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATATCAGGCAGTTTAACTGTTCTCCACACCCCT
ACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTGCCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGA
TGACGAACTGATTTCTGATGATGAAGCAGAAGGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGAGCCATTGGGAAGATGGCACGGGTC
TTTTCAATTCTTCGGCAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCAAGCAGA
CTATCGAGACAGCCATCAGAGGGTTCTCGCTTCAGCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCTGGACCGAATTAATGAGCGAATGCCACCCCG
AAAGGATAGCATACACGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCTGCGCACAGGAGCGACCAAGGGAAGAAAGCCCATTCATGA
CTTAGAGTCCTGCCGTGGCTCAGGTGGATCTAAAACTCAAGAACAAATTCTATTTATTTATTATTGGAAAATGAAAAGCAACTCAAAACAACTTCAACGT
GGAGGTGCATTTATAATTCAGTCTGCATTTATTCTGTAAAAAGGTGGCTGTTTTATAAATTCTTTTAATTTATGTTCAATATATATAAAAAGTGCATCTG
TTTTGTTTTTCCCTTTTTTCTCCATAATTTTAAGAAATGAATCTGATTGTTGTCAACACATTTGTGAAGTCTTGTGCTATAAAGGGGAACTTCCCCTAAT
AAAAGGGCCTTGGAAACCTCAAACCTGGGTTTCTGACTTGAA
  • PPP3CC.u.f.15 (forward primer)
  • PPP3CC.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

VPGPGRGWQERRGRLRGGLAEKKRKWAVSSRDP
EPEEPRAARAPSSTASREGGRVGRAALGAGGPAG
SARTAGPLGSCESGARGRACVHEGPGREQPRPS
RSPPLAAVAVGAEAVFPALAAGASQESGRWALV
LSVASEAGAAVAAPFWCSDTAEEPGPGTAG*RLR
AAKAARGEGGGRGVGARPAGSWSLEEAEGTMSG
RRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGK
PKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQ
EKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGS
PSNTRYLFLGDYVDRGYFSIECVLYLWSLKINH
PKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQV
YDACMETFDCLPLAALLNQQFLCVHGGMSPEIT
SLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYG
NEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNL
LSIIRAHEAQDAGYRMYRKSQATGFPSLITIFS
APNYLDVYNNKAAVLKYENNVMNIRQFNCSPHP
YWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSD
DELISDDEAEGSTTVRKEIIRNKIRAIGKMARV
FSILRQESESVLTLKGLTPTGTLPLGVLSGGKQ
TIETAIRGFSLQHKIRSFEEARGLDRINERMPPR
KDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS*
LRVLPWLRWI*NSRTNSIYLLLENEKQLKTTST
WRCIYNSVCIYSVKRWLFYKFF*FMFNIYKKCIC
FVFPFFLHNFKK*I*LLSTHL*SLVL*RGTSPN
KRALETSNLGF*LE

Frame 1

FRARVGAGRREGAGCGEGWLRRSENGRLAAGTR
SRRSREQRVRRALLPRGKAEGWGGRRSGREARP
GPLGQRGRWEVVRAALGGALACTRARAASSRGRP
GRHP*QRSRSVPKRCSPP*PLAPPKRAAGGPSS
CQWRRRPALRWPRPSGARTPLRSRGRARLADGS
GQLRLPEEKAAAAA*AHVRRAPGAWRRPRGPCPG
GASTSPPPTASSKLSPFLQPNGLLSRKYLRMGN
LKLMF*KTIW*RKDDWKRK*P*R*SMMGLPS*G
KRRL**K*MLQSQYVVIFMDNSLT**SYLKLEDH
LVTHATSFWVTMWTEAISV*SVCCIYGV*RLII
PKHCFCFGEIMNAGILQTISPSNRNVESNIRNR
CMMPVWRHLTVFLLLPS*TSSFSVYMEECHLKLL
L*MTLGN*TGLRNLPPLDLCVTCFGLIPQRIMA
MRRPWSTIPTTLSEGALISTVTLQFVNFCRTII
YYQLSEPMKPKMLGIECTGRAKPQAFHHLLQFSL
PPIT*MSITIKLLC*NMKTMS*ISGSLTVLHTP
TGFQTLWMFSHGLCLLLGKKSQRCW*MCSTYAL
MTN*FLMMKQKEALQFVRRSSGIRSEPLGRWHGS
FQFFGKKVRVC*LSRA*LPQAHSLWASSQEASR
LSRQPSEGSRFSTRSGVLKKREVWTELMSECHP
ERIAYTLVGQ*NL*PQHTHMLRTGATKGRKPIHD
LESCRGSGGSKTQEQILFIYYWKMKSNSKQLQR
GGAFIIQSAFIL*KGGCFINSFNLCSIYIKSAS
VLFFPFFSIILRNESDCCQHICEVLCYKGELPLI
KGPWKPQTWVSDL

Frame 2

SGPG*GLAGEKGPAAGRAG*EEAKMGG*QQGPG
AGGAESSACAELYCLAGRRKGGEGGARGGRPGR
VR*DSGAAGKL*ERRSGARLRARGPGPRAAAAV
PVATLSSGRGRCRSGVPRLSRWRLPRERPVGPRP
VSGVGGRRCGGRALLVLGHR*GAGAGHGWLTAP
GS*GCPRRRRRPRRRRTSGGLLEPGGGRGDHVR
EALPPLHHRPRHQSCPLSSNPTAYFQGSI*EWET
*S*CFKKPFGKGRTTGRGSSLKDNQ*WGCHPEA
REDYDRSRCSNHSMW*YSWTIL*PNEVI*SWRI
T**HTLPLSG*LCGQRLFQYRVCAVFMEFKD*SS
QNIVSASGKS*MQASYRLFHLQTGMSNQIFGTG
V*CLYGDI*LSSSCCPLKPAVSLCTWRNVT*NY
FFR*H*EIRQVYGTSRLWTCV*PALV*SLRGLWQ
*EDLGALYPQHCPRVLLFLQLPCSL*IFAEQ*F
TINYQSP*SPRCWVSNVQEEPSHRLSITYYNFL
CPQLPRCL*Q*SCCVEI*KQCHEYQAV*LFSTPL
LASKLYGCFHMVFAFCWGKSHRDAGKCAQHML*
*RTDF***SRRKHYSS*GDHQE*DQSHWEDGTG
LFNSSARK*ECADSQGPDSHRHTPSGRPLRRQAD
YRDSHQRVLASAQDPEF*RSARSGPN**ANATP
KG*HTRWWANEICNLSTLTCCAQERPREESPFM
T*SPAVAQVDLKLKNKFYLFIIGK*KATQNNFNV
EVHL*FSLHLFCKKVAVL*ILLIYVQYI*KVHL
FCFSLFSP*F*EMNLIVVNTFVKSCAIKGNFP*
*KGLGNLKPGFLT*

Sequence PPP3CC.aAug05

GTTCCGGGCCCGGGTAGGGGCTGGCAGGAGAGAAGGGGCCGGCTGCGGGGAGGGCTGGCTGAGAAGAAGCGAAAATGGGCGGTTAGCAGCAGGGACCCGG
AGCCGGAGGAGCCGAGAGCAGCGCGTGCGCCGAGCTCTACTGCCTCGCGGGAAGGCGGAAGGGTGGGGAGGGCGGCGCTCGGGGCGGGAGGCCCGGCCGG
GTCCGCTAGGACAGCGGGGCCGCTGGGAAGTTGTGAGAGCGGCGCTCGGGGGCGCGCTTGCGTGCACGAGGGCCCGGGCCGCGAGCAGCCGCGGCCGTCC
CGGTCGCCACCCTTAGCAGCGGTCGCGGTCGGTGCCGAAGCGGTGTTCCCCGCCTTAGCCGCTGGCGCCTCCCAAGAGAGCGGCCGGTGGGCCCTCGTCC
TGTCAGTGGCGTCGGAGGCCGGCGCTGCGGTGGCCGCGCCCTTCTGGTGCTCGGACACCGCTGAGGAGCCGGGGCCGGGCACGGCTGGCTGACGGCTCCG
GGCAGCTAAGGCTGCCCGAGGAGAAGGCGGCGGCCGCGGCGTAGGCGCACGTCCGGCGGGCTCCTGGAGCCTGGAGGAGGCCGAGGGGACCATGTCCGGG
AGGCGCTTCCACCTCTCCACCACCGACCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCCAACGGCTTACTTTCAAGGAAGTATTTGAGAATGGGAAAC
CTAAAGTTGATGTTTTAAAAAACCATTTGGTAAAGGAAGGACGACTGGAAGAGGAAGTAGCCTTAAAGATAATCAATGATGGGGCTGCCATCCTGAGGCA
AGAGAAGACTATGATAGAAGTAGATGCTCCAATCACAGTATGTGGTGATATTCATGGACAATTCTTTGACCTAATGAAGTTATTTGAAGTTGGAGGATCA
CCTAGTAACACACGCTACCTCTTTCTGGGTGACTATGTGGACAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATTTATGGAGTTTAAAGATTAATCATC
CCAAAACATTGTTTCTGCTTCGGGGAAATCATGAATGCAGGCATCTTACAGACTATTTCACCTTCAAACAGGAATGTCGAATCAAATATTCGGAACAGGT
GTATGATGCCTGTATGGAGACATTTGACTGTCTTCCTCTTGCTGCCCTCTTAAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACT
TCTTTAGATGACATTAGGAAATTAGACAGGTTTACGGAACCTCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGGATTATGGCA
ATGAGAAGACCTTGGAGCACTATACCCACAACACTGTCCGAGGGTGCTCTTATTTCTACAGTTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTT
ACTATCAATTATCAGAGCCCATGAAGCCCAAGATGCTGGGTATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCT
GCCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATATCAGGCAGTTTAACTGTTCTCCACACCCCT
ACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTGCCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGA
TGACGAACTGATTTCTGATGATGAAGCAGAAGGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGAGCCATTGGGAAGATGGCACGGGTC
TTTTCAATTCTTCGGCAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCAAGCAGA
CTATCGAGACAGCCACAGTAGAAGCGGTAGAGGCCCGGGAAGCCATCAGAGGGTTCTCGCTTCAGCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCT
GGACCGAATTAATGAGCGAATGCCACCCCGAAAGGATAGCATACACGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCTGCGCACAGG
AGCGACCAAGGGAAGAAAGCCCATTCATGACTTAGAGTCCTGCCGTGGCTCAGGTGGATCTAAAACTCAAGAACAAATTCTATTTATTTATTATTGGAAA
ATGAAAAGCAACTCAAAACAACTTCAACGTGGAGGTGCATTTATAATTCAGTCTGCATTTATTCTGTAAAAAGGTGGCTGTTTTATAAATTCTTTTAATT
TATGTTCAATATATATAAAAAGTGCATCTGTTTTGTTTTTCCCTTTTTTCTCCATAATTTTAAGAAATGAATCTGATTGTTGTCAACACATTTGTGAAGT
CTTGTGCTATAAAGGGGAACTTCCCCTAATAAAAGGGCCTTGGAAACCTCAAACCTGGGTTTCTGACTTGAA
  • PPP3CC.u.f.15 (forward primer)
  • PPP3CC.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

VPGPGRGWQERRGRLRGGLAEKKRKWAVSSRDP
EPEEPRAARAPSSTASREGGRVGRAALGAGGPAG
SARTAGPLGSCESGARGRACVHEGPGREQPRPS
RSPPLAAVAVGAEAVFPALAAGASQESGRWALV
LSVASEAGAAVAAPFWCSDTAEEPGPGTAG*RLR
AAKAARGEGGGRGVGARPAGSWSLEEAEGTMSG
RRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGK
PKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQ
EKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGS
PSNTRYLFLGDYVDRGYFSIECVLYLWSLKINH
PKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQV
YDACMETFDCLPLAALLNQQFLCVHGGMSPEIT
SLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYG
NEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNL
LSIIRAHEAQDAGYRMYRKSQATGFPSLITIFS
APNYLDVYNNKAAVLKYENNVMNIRQFNCSPHP
YWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSD
DELISDDEAEGSTTVRKEIIRNKIRAIGKMARV
FSILRQESESVLTLKGLTPTGTLPLGVLSGGKQ
TIETATVEAVEAREAIRGFSLQHKIRSFEEARGL
DRINERMPPRKDSIHAGGPMKSVTSAHSHAAHR
SDQGKKAHS*LRVLPWLRWI*NSRTNSIYLLLE
NEKQLKTTSTWRCIYNSVCIYSVKRWLFYKFF*F
MFNIYKKCICFVFPFFLHNFKK*I*LLSTHL*S
LVL*RGTSPNKRALETSNLGF*LE

Frame 1

FRARVGAGRREGAGCGEGWLRRSENGRLAAGTR
SRRSREQRVRRALLPRGKAEGWGGRRSGREARP
GPLGQRGRWEVVRAALGGALACTRARAASSRGRP
GRHP*QRSRSVPKRCSPP*PLAPPKRAAGGPSS
CQWRRRPALRWPRPSGARTPLRSRGRARLADGS
GQLRLPEEKAAAAA*AHVRRAPGAWRRPRGPCPG
GASTSPPPTASSKLSPFLQPNGLLSRKYLRMGN
LKLMF*KTIW*RKDDWKRK*P*R*SMMGLPS*G
KRRL**K*MLQSQYVVIFMDNSLT**SYLKLEDH
LVTHATSFWVTMWTEAISV*SVCCIYGV*RLII
PKHCFCFGEIMNAGILQTISPSNRNVESNIRNR
CMMPVWRHLTVFLLLPS*TSSFSVYMEECHLKLL
L*MTLGN*TGLRNLPPLDLCVTCFGLIPQRIMA
MRRPWSTIPTTLSEGALISTVTLQFVNFCRTII
YYQLSEPMKPKMLGIECTGRAKPQAFHHLLQFSL
PPIT*MSITIKLLC*NMKTMS*ISGSLTVLHTP
TGFQTLWMFSHGLCLLLGKKSQRCW*MCSTYAL
MTN*FLMMKQKEALQFVRRSSGIRSEPLGRWHGS
FQFFGKKVRVC*LSRA*LPQAHSLWASSQEASR
LSRQPQ*KR*RPGKPSEGSRFSTRSGVLKKREV
WTELMSECHPERIAYTLVGQ*NL*PQHTHMLRTG
ATKGRKPIHDLESCRGSGGSKTQEQILFIYYWK
MKSNSKQLQRGGAFIIQSAFIL*KGGCFINSFN
LCSIYIKSASVLFFPFFSIILRNESDCCQHICEV
LCYKGELPLIKGPWKPQTWVSDL

Frame 2

SGPG*GLAGEKGPAAGRAG*EEAKMGG*QQGPG
AGGAESSACAELYCLAGRRKGGEGGARGGRPGR
VR*DSGAAGKL*ERRSGARLRARGPGPRAAAAV
PVATLSSGRGRCRSGVPRLSRWRLPRERPVGPRP
VSGVGGRRCGGRALLVLGHR*GAGAGHGWLTAP
GS*GCPRRRRRPRRRRTSGGLLEPGGGRGDHVR
EALPPLHHRPRHQSCPLSSNPTAYFQGSI*EWET
*S*CFKKPFGKGRTTGRGSSLKDNQ*WGCHPEA
REDYDRSRCSNHSMW*YSWTIL*PNEVI*SWRI
T**HTLPLSG*LCGQRLFQYRVCAVFMEFKD*SS
QNIVSASGKS*MQASYRLFHLQTGMSNQIFGTG
V*CLYGDI*LSSSCCPLKPAVSLCTWRNVT*NY
FFR*H*EIRQVYGTSRLWTCV*PALV*SLRGLWQ
*EDLGALYPQHCPRVLLFLQLPCSL*IFAEQ*F
TINYQSP*SPRCWVSNVQEEPSHRLSITYYNFL
CPQLPRCL*Q*SCCVEI*KQCHEYQAV*LFSTPL
LASKLYGCFHMVFAFCWGKSHRDAGKCAQHML*
*RTDF***SRRKHYSS*GDHQE*DQSHWEDGTG
LFNSSARK*ECADSQGPDSHRHTPSGRPLRRQAD
YRDSHSRSGRGPGSHQRVLASAQDPEF*RSARS
GPN**ANATPKG*HTRWWANEICNLSTLTCCAQ
ERPREESPFMT*SPAVAQVDLKLKNKFYLFIIGK
*KATQNNFNVEVHL*FSLHLFCKKVAVL*ILLI
YVQYI*KVHLFCFSLFSP*F*EMNLIVVNTFVK
SCAIKGNFP**KGLGNLKPGFLT*

Sequence PPP3CC.eAug05

TGACTATGTGGACAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATTTATGGAGTTTAAAGATTAATCATCCCAAAACATTGTTTCTGCTTCGGGGAAAT
CATGAATGCAGGCATCTTACAGACTATTTCACCTTCAAACAGGAATGTCGAATCAAATATTCGGAACAGGTGTATGATGCCTGTATGGAGACATTTGACT
GTCTTCCTCTTGCTGCCCTCTTAAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACTTCTTTAGATGACATTAGGAAATTAGACAG
GTTTACGGAACCTCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGGATTATGGCAATGAGAAGACCTTGGAGCACTATACCCAC
AACACTGTCCGAGGGTGCTCTTATTTCTACAGTTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTTACTATCAATTATCAGAGCCCATGAAGCCC
AAGATGCTGGGTATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCTGCCCCCAATTACCTAGATGTCTATAACAA
TAAAGAGTCTGCGACACATTCTTTTGATTATCCTCAATGATGGGAAATCTTCGTCCTTTGAGGGTGGATTTGATTTTTGGACAGCCAAAAGTCATTCGGA
GTCAAGACTGATAAATAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATATCAGGCAGTTTAACTGTTCTCCACACCCCTACTGGCTTCCAAACT
TTATGGATGTTTTCACATGGTCTTTGCCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGATGACGAACTGATTTC
TGATGATGAAGCAGAAGGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGAGCCATTGGGAAGATGGCACGGGTCTTTTCAATTCTTCGG
CAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTGACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCAAGCAGACTATCGAGACAGCCA
TCAGAGGGTTCTCGCTTCAGCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCTGGACCGAATTAATGAGCGAATGCCACCCCGAAAGGATAGCATACA
CGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCTGCGCACAGGAGCGACCAAGGGAAGAAAGCCCATTCATGACTTAGAGTCCTGCCG
TGGCTCAGGTGGATCTAAAACTCAAGAACAAATTCTATTTATTTATTATTGGAAAATGAAAAGCAACTCAAAACAACTTCAACGTGGAGGTGCATTTATA
ATTCAGTCTGCATTTATTCTGTAAAAAGGTGGCTGTTTTATAAATTCTTTTAATTTATGTTCAATATATATAAAAAGTGCATCTGTTTTGTTTTTCCCTT
TTTTCTCCATAATTTTAAGAAATGAATCTGATTGTTGTCAACACATTTGTGAAGTCTTGTGCTATAAAGGGGAACTTCCCCTAATAAAAGGGCCTTGGAA
ACCTCAAACCTGGGTTTCTGACTTGAA
  • PPP3CC.u.f.15 (forward primer)
  • PPP3CC.u.r.16 (reverse primer)
  • Single exon skipping

Frame 0

*LCGQRLFQYRVCAVFMEFKD*SSQNIVSASGK
S*MQASYRLFHLQTGMSNQIFGTGV*CLYGDI*L
SSSCCPLKPAVSLCTWRNVT*NYFFR*H*EIRQ
VYGTSRLWTCV*PALV*SLRGLWQ*EDLGALYP
QHCPRVLLFLQLPCSL*IFAEQ*FTINYQSP*SP
RCWVSNVQEEPSHRLSITYYNFLCPQLPRCL*Q
*RVCDTFF*LSSMMGNLRPLRVDLIFGQPKVIR
SQD**ISCCVEI*KQCHEYQAV*LFSTPLLASKL
YGCFHMVFAFCWGKSHRDAGKCAQHML**RTDF
***SRRKHYSS*GDHQE*DQSHWEDGTGLFNSS
ARK*ECADSQGPDSHRHTPSGRPLRRQADYRDSH
QRVLASAQDPEF*RSARSGPN**ANATPKG*HT
RWWANEICNLSTLTCCAQERPREESPFMT*SPA
VAQVDLKLKNKFYLFIIGK*KATQNNFNVEVHL*
FSLHLFCKKVAVL*ILLIYVQYI*KVHLFCFSL
FSP*F*EMNLIVVNTFVKSCAIKGNFP**KGLG
NLKPGFLT*

Frame 1

DYVDRGYFSIECVLYLWSLKINHPKTLFLLRGN
HECRHLTDYFTFKQECRIKYSEQVYDACMETFD
CLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDR
FTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTH
NTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEA
QDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNN
KESATHSFDYPQ*WEIFVL*GWI*FLDSQKSFG
VKTDK*AAVLKYENNVMNIRQFNCSPHPYWLPN
FMDVFTWSLPFVGEKVTEMLVNVLNICSDDELIS
DDEAEGSTTVRKEIIRNKIRAIGKMARVFSILR
QESESVLTLKGLTPTGTLPLGVLSGGKQTIETA
IRGFSLQHKIRSFEEARGLDRINERMPPRKDSIH
AGGPMKSVTSAHSHAAHRSDQGKKAHS*LRVLP
WLRWI*NSRTNSIYLLLENEKQLKTTSTWRCIY
NSVCIYSVKRWLFYKFF*FMFNIYKKCICFVFPF
FLHNFKK*I*LLSTHL*SLVL*RGTSPNKRALE
TSNLGF*LE

Frame 2

TMWTEAISV*SVCCIYGV*RLIIPKHCFCFGEI
MNAGILQTISPSNRNVESNIRNRCMMPVWRHLT
VFLLLPS*TSSFSVYMEECHLKLLL*MTLGN*T
GLRNLPPLDLCVTCFGLIPQRIMAMRRPWSTIPT
TLSEGALISTVTLQFVNFCRTIIYYQLSEPMKP
KMLGIECTGRAKPQAFHHLLQFSLPPIT*MSIT
IKSLRHILLIILNDGKSSSFEGGFDFWTAKSHSE
SRLINKLLC*NMKTMS*ISGSLTVLHTPTGFQT
LWMFSHGLCLLLGKKSQRCW*MCSTYALMTN*F
LMMKQKEALQFVRRSSGIRSEPLGRWHGSFQFFG
KKVRVC*LSRA*LPQAHSLWASSQEASRLSRQP
SEGSRFSTRSGVLKKREVWTELMSECHPERIAY
TLVGQ*NL*PQHTHMLRTGATKGRKPIHDLESCR
GSGGSKTQEQILFIYYWKMKSNSKQLQRGGAFI
IQSAFIL*KGGCFINSFNLCSIYIKSASVLFFP
FFSIILRNESDCCQHICEVLCYKGELPLIKGPWK
PQTWVSDL

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