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Sequences

Sequence OPA1.gAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTTGTTAGGTT CTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGA AGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATGACAAA GGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATACGCAAGATC ATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGA GATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTACCAAAGAA GAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAA AAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACTATTTTCAG TATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGT CAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGATAATTG AAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAATATGAAGA AGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGG AAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCGCCTGCGGG AACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCT TCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACACCACAGTG GATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAG AACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCGGAGGA CAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAG GCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTG TTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTCCGGAAGAA CCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGT AACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACAGCGCATGC TTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGA TGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATT GAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCAGCCTCTTT TTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCCTCTCGAAT GGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTCCTTGAAGA TAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAGAAATACAA TGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGTGTCTGTAG TATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAGGTTAATTT GGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTTGATTCAGT TGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCTCCTGTTGA CAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGGTATCAGAA AAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAAATTTAGTT CAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGCAAAGAAAT GCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGATTTTACAG TTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTTGTGATCCC TCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGATAAGAAAA GTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATAGAAAGGGA GGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAATGAATATTT TTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAAGAAAGACC TTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGATGGAGGGA GATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGACGCCCTCT CCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAAGCTAGGTA TTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATGATTCTAAT TTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTGGACAAAGC TCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGTGCACAGCA CGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTGCCCTCTTT GTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGGAACATGGC AGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATAATTTTGTA TTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGTTCTCTAAG CTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTTGAAACTTA CCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTGCCTGAGCT TGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTTTTCTGTTTTAA CGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCTTTTCTCTGAAT GCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTTTGTTATTTTCC TTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OPA1.u.f.9 (forward primer)
OPA1.u.r.8 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFGHKLVSEVIGASDLLLLLG SPEETAFRATDRGSESDKHFRKVSDKEKIDQLQE ELLHTQLKYQRILERLEKENKELRKLVLQKDDK GIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQD HLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGE MMTRSPVKVTLSEGPHHVALFKDSSREFDLTKE EDLAALRHEIELRMRKNVKEGCTVSPETISLNV KGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFS ISKAYMQNPNAIILCIQDGSVDAERSIVTDLVS QMDPHGRRTIFVLTKVDLAEKNVASPSRIQQII EGKLFPMKALGYFAVVTGKGNSSESIEAIREYEE EFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFW KMVRESVEQQADSFKATRFNLETEWKNNYPRLR ELDRNELFEKAKNEILDEVISLSQVTPKHWEEIL QQSLWERVSTHVIENIYLPAAQTMNSGTFNTTV DIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMT EPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAED SLRVIQHNALEDRSISDKQQWDAAIYFMEEALQ ARLKDTENAIENMVGPDWKKRWLYWKNRTQEQC VHNETKNELEKMLKCNEEHPAYLASDEITTVRKN LESRGVEVDPSLIKDTWHQVYRRHFLKTALNHC NLCRRGFYYYQRHFVDSELECNDVVLFWRIQRM LAITANTLRQQLTNTEVRRLEKNVKEVLEDFAED GEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFI EALHQEK*IKIVLIISSAYI*RTKTSTSFVQPL FLLLFHLSKHTIKSWDKNNRCMLRAL*PSAASRM EEQW*WINILFCCTKVTNRNNIIGMAIRFSP*R *ETCSLFVVLFVVALV*WIN*GCSMFSFFSRNT MLGVLK*NLYSNCLKLSIVYKITVAC*IIVCVCS ILFPETI*P**FSLYSPHERKMGNRRTLKTG*F GL*RSVKEISTLHSQRRNLPWKPSQYQLTYLIQ LLFSHSLYPFEIDLF*MLYTYVRLSVNSGFSPVD RATGL*HRMRKIKDNQLTNFI*NIIKHFN*VSE KGFLS*DYFK*KLFQQLK*C*PSPSQLNKEKFS SIYCNLITILPSQHFHVF*INIF*LAKGHSSKEM LSLLKMSISFAAFSLSLYFVNKSVHCVMFLILQ FAKSYFLGVAFW*QPHCYSPFYCFTSMHASL*S LKM*HLVCSVEDMKKYFRNQEFNVCLFYTV**EK *VQP*AAQMRW*MHSQELFLFLLGLGTGVNRKG GELYYVLYTD*EMTLLGTPL*PVLTG*CMLMNI FAVVKHNNYNYTSISKE*NRTIFIYFCQL*KERP SAVFPQISPKEKANISHYCYHIPLYKF*KEMEG DLHFFEEISIVTYVNR**QLILLKVPHESPDAL SMPHSNVASFMGFFFFFLADLILSMLYFSLKARY LSTADVIERK*NSVSRVNPVSCVCSSKVRNDSN LNKKILNIQKLNSN*PQNHLFLCQNLQRVEWTK LCMED*TTVNR*YPNLIWLGLQF*KSVYLGSAQH EISGPCSFPV*PTVL*LRVEQSTFLVVYDIALF VFPFSTVFSADFFLQIIQPPNANE*YKNK*GTW QRVVLPSLTMWEFDIG*ESEYRFKR*NL*LIILY LFILDVCI*GKKSGIFAFQ*RGSK*WFFSQFSK LVYVIALAVWKCALKYAYLLNDHGYMLLRYLKL TLFSLVHCE*TLYYFFKTFTLRVDIIATYILPEL DQRSFV*MSN*KIFKSHYNSIIGEHLLNFFLF* RGKEKPVYLGSLFDPIV*PDSWSNKLSVWLFSE CLNFTCLAFHSCTPWSTGAFFHIVVLVKTFCYFP W*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILVTNWLVKS*ELLTYFSC*V LRKKRRLEQQIVDLKVTSILERCQTKRKLTNFR KNFCTLS*SIRESWND*KRRTKN*EN*YCRKMTK AFIIESLRNL*LTCILKFLMFSLIMMPVIIRKI ICHGLLWLEIRVLERLVCWK*LPKLEYSQEDLG R**HVLQLR*L*VKVLTMWPYLKIVLGSLILPKK KILQH*DMK*NFE*GKM*KKAVPLALRPYP*M* KALDYRGWCLLTYQV*LIL*HQAWLLTQRKLFS VSAKLTCRILMPSYCVFKMDLWMLNAVLLQTWSV KWTLMEGEPYSF*PK*TWQRKM*PVQAGFSR*L KESSSQ*KL*VILLL*QEKGTALKALKL*ENMK KSFFRIQSS*RQAC*RHTK*LQEI*ALQYQTAFG KWYESLLNNRLIVSKQHVLTLKLNGRITILACG NLTGMNYLKKLKMKSLMKLSV*ARLHQNIGRKS FNNLCGKEYQLM*LKTSTFQLRRP*IQELLTPQW ISSLNSGLINNFLIKQ*RLLGRPYKKNFPAL*Q NRKGKSMMTYLINLKRLLRKKVLNDTSGMTLRR TA*GLFNTMLWKTDPYLINSNGMQLFILWKRLCR LVSRILKMQLKTWWVQTGKRGGYTGRIGPKNSV FTMKPRMNWRRC*NVMRSTQLILQVMK*PQSGR TLNPEE*K*IQA*LRILGIKFIEDIF*KQL*TIV TFVEEVFITTKGIL*ILSWNAMMWSCFGVYSAC LLSPQIL*GNNLQILKLGD*RKMLKRYWKILLK MVRRRLNCLLVNAFNWRKTSRKLEKFKKNLMLSL KLFIRRNKLKSYS*SALHTSEEQKHQRLLSSLF FFCCSTFLNIQ*SHGIKIIDVCYGRFNHQLPLE WKNSGNGLTSYFVVLK*QIGII*LVWPLGSVLED KKLVLCLLSYLWWHSFNGLTEVAQCSVSFPEIQ C*VF*NKTYIAIV*SYQLYIKSQ*PAKSLYVSV VFYSQKLFDHDNSVYIHHMKEKWVTEEPLKQVNL DCNVQ*KKFQPFIASEEICLGSQVSTSLPI*FS CCFLTLYIHLKLIYFRCCILTLGFLLIVVFLLL TEPPDYDTG*GRLRIIN*LISFRILSNISTRYQK KAFFHKTILNRNYFNN*SNVDHPPLS*IKKNLV QFIAI*LQYYLHNIFMCFK*IFFNWLKDIQAKK CFLYLKCLSHLLPFH*AFTLLIKVSIV*CF*FYS LLNLIFLELLFGNSPIATPHFIVLHQCMLRCDP SRCNTWYARLRI*KNTSETRNSMYVCFILFDKK SRSSLKQHRCAGRCIVRNFFYFF*V*GQE*IERE ESSIMFYTQIRR*PYWVHPSNQCLQVNAC**IF LQL*SITITTTHLFLKNKTGPYLFTSVNYRKKD LQLYFHRFLPRKRLILVTTVITSLCISFKKRWRE IFISLRRSVL*RM*IDDNN*YY*KSHMRVLTPS PCPTVMWLLSWVFFSSF*LISS*ACFIFP*KLG IYQLQMLLKENKIQSQE*TLCLVSVVQKSEMILI *TKRY*IYRS*IRTSHRIICFYVRICKEWSGQS SVWKTEQL*IDDIQT*FG*DFNFKNQCT*AVHS TK*VALAASPFNPLCYSCGWNSQPF**FMILPSL YSHFLQFFPQTSFCKLFSLQMQMNDIKISREHG REWCFPASQCGNLT*DESQSIGLKDKIFS**FC IYLF*MYVSEERNLVFLLSNKGDQSNGFSLSSLS WSML*L*QYGNVL*NMLTF*MIMAICC*DI*NL PCFHLCTVNELCIIFLKPSHYV*ILLQLIFCLS LIKGHLCR*VIKKYLNHIIILLLESIF*IFFCFN EGKRNLYT*GHYLTP*YNQIHGLTSSQCGFSLN A*ISHALHFTVVLHGQPVLFFTSWYLSKHFVIF LGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRFWSQIG**SHRSF*PTSLVRF SGRNGV*SNRSWI*K*QAF*KGVRQREN*PTSG RTSAHSVEVSENLGTIRKGEQRIEKISIAER*Q RHSS*KA*EIFD*HVF*SS*CSL*L*CQL*YARS SATGCCGWRSECWKD*CVGNDCPSSNIPKRIWG DDDTFSS*GDSE*RSSPCGPI*R*FSGV*SYQR RRSCSIKT*NRTSNEEKCERRLYR*P*DHILKCK RPWTTEDGAC*LTRCD*YCDIRHGS*HKGNYFQ YQQSLHAES*CHHTVYSRWICGC*TQYCYRLGQ SNGPSWKENHIRFDQSRPGREKCSQSKQDSADN* RKALPNESFRLFCCCNRKREQL*KH*SYKRI*R RVFSEFKAPKDKHAKGTPSDYKKFKPCSIRLLL ENGTRVC*TTG**FQSNTF*P*N*MEE*LSSPAG T*PE*TI*KS*K*NP**SYQSEPGYTKTLGGNP STIFVGKSINSCD*KHLPSSCADHEFRNF*HHS GYQA*TVD**TTS**SSRGCLGDPTRRIFPLYDR TERERA**HI**T*RGC*GRKY*TTQVE*LCGG QLEGYSTQCFGRPIHI**TAMGCSYLFYGRGSA GSSQGY*KCN*KHGGSRLEKEVVILEESDPRTVC SQ*NQE*IGEDVEM**GAPSLSCK**NNHSPEE P*IPRSRSRSKLD*GYLASSL*KTFFKNSSKPL *PLSKRFLLLPKAFCRF*VGMQ*CGLVLAYTAHA CYHRKYFKATTYKY*S*AIREKC*RGIGRFC*R W*EED*IAYW*TRSTGGRPQES*RNSRKT*CFH *SSSSGEIN*NRTHNQLCIHLKNKNINVFCPASF SSAVPPF*TYNKVMG*K*SMYVTGALTISCLSN GRTVVMD*HPILLY*SDKSE*YNWYGH*VQSLK IRNLFSVCCLICGGTRLMD*LRLLNVQFLFQKYN ARCFEIKLI*QLFKVINCI*NHSSLLNHCMCL* YSIPRNYLTMIIQFIFTT*KKNG*QKNP*NRLI WIVTFSERNFNPS*PAKKFALEAKSVPAYLFDSV AVFSLSISI*N*FILDVVYLR*AFC**WFFSC* QSHRIMTQDEED*G*SID*FHLEYYQTFQLGIR KRLSFIRLF*IEIISTIKVMLTIPLSAE*RKI*F NLLQFNYNTTFTTFSCVLNKYFLIG*RTFKQRN AFFT*NVYLICCLFTKPLLC**KCPLCDVFDFT VC*ILFSWSCFLVTAPLLLPILLFYINACFVVIP QDVTLGMLG*GYEKILPKPGIQCMFVLYCLIRK VGPALSSTDALVDA*SGTFFISFRSRDRSE*KG RRALLCSIHRLGDDLTGYTPLTSAYRLMHVNEYF CSCKA*QLQLHIYF*RIKQDHIYLLLSTIERKT FSCISTDFSQGKG*Y*SLLLSHPFV*VLKRDGG RSSFL*GDQYCNVCE*MITINITKSPT*ES*RPL HAPQ*CGFFHGFFFLLFS*SHPKHALFFLES*V FINCRCY*KKIKFSLKSKPCVLCL*FKSQK*F* FKQKDTKYTEVKFELATESFVFMSEFAKSGVDKA LYGRLNNCK*MISKLNLARTSILKISVPRQCTA RNKWPLQLPRLTHCAIVAGGTVNLSSSL*YCPL CIPIFYSFFRRLLSANYSASKCK*MI*K*VGNMA ESGASQPHNVGI*HRMRVRV*V*KIKSLVNNFV FIYSRCMYLRKEIWYFCFPIKGIKVMVFLSVL* AGLCYSSSSMEMCFKICLPFE*SWLYVVEIFETY LVFTCAL*MNFVLFF*NLHITCRYYCNLYFA*A *SKVICVDE*LKNI*ITL*FYYWRASFKFFSVL TRERETCIPRVII*PHSITRFMV*QALSVAFL*M LEFHMPCISQLYSMVNRCFFSHRGTCQNILLFS LVKYIKKVF*FHFAA

Sequence OPA1.eAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTT GTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAA CTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAG ATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATAC GCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGA TCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTA CCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTT AAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACT ATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACT TGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCA GATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAA TATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACT GCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCG CCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAG GAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACA CCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTT TATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTT GCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGG CTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGA ACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTC CGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAA ACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACA GCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTT GCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATG CTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCA GCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCC TCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTC CTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAG AAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGT GTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAG GTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTT GATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCT CCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGG TATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAA ATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGC AAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGA TTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTT GTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGA TAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATA GAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAAT GAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAA GAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGA TGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGA CGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAA GCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATG ATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTG GACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGT GCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTG CCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGG AACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATA ATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGT TCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTT GAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTG CCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTTTTC TGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCTTTT CTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTTTGT TATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OPA1.u.f.9 (forward primer)
OPA1.u.r.8 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGHKLVSEVIGASDLLLL LGSPEETAFRATDRGSESDKHFRKVSDKEKIDQ LQEELLHTQLKYQRILERLEKENKELRKLVLQK DDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNT QDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRG SGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISL NVKGPGLQRMVLVDLPGVINTVTSGMAPDTKET IFSISKAYMQNPNAIILCIQDGSVDAERSIVTD LVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQ IIEGKLFPMKALGYFAVVTGKGNSSESIEAIRE YEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSD CFWKMVRESVEQQADSFKATRFNLETEWKNNYPR LRELDRNELFEKAKNEILDEVISLSQVTPKHWE EILQQSLWERVSTHVIENIYLPAAQTMNSGTFN TTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRF MTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDF AEDSLRVIQHNALEDRSISDKQQWDAAIYFMEE ALQARLKDTENAIENMVGPDWKKRWLYWKNRTQE QCVHNETKNELEKMLKCNEEHPAYLASDEITTV RKNLESRGVEVDPSLIKDTWHQVYRRHFLKTAL NHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQ RMLAITANTLRQQLTNTEVRRLEKNVKEVLEDF AEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLD AFIEALHQEK*IKIVLIISSAYI*RTKTSTSFVQ PLFLLLFHLSKHTIKSWDKNNRCMLRAL*PSAA SRMEEQW*WINILFCCTKVTNRNNIIGMAIRFS P*R*ETCSLFVVLFVVALV*WIN*GCSMFSFFSR NTMLGVLK*NLYSNCLKLSIVYKITVAC*IIVC VCSILFPETI*P**FSLYSPHERKMGNRRTLKT G*FGL*RSVKEISTLHSQRRNLPWKPSQYQLTYL IQLLFSHSLYPFEIDLF*MLYTYVRLSVNSGFS PVDRATGL*HRMRKIKDNQLTNFI*NIIKHFN* VSEKGFLS*DYFK*KLFQQLK*C*PSPSQLNKEK FSSIYCNLITILPSQHFHVF*INIF*LAKGHSS KEMLSLLKMSISFAAFSLSLYFVNKSVHCVMFL ILQFAKSYFLGVAFW*QPHCYSPFYCFTSMHASL *SLKM*HLVCSVEDMKKYFRNQEFNVCLFYTV* *EK*VQP*AAQMRW*MHSQELFLFLLGLGTGVN RKGGELYYVLYTD*EMTLLGTPL*PVLTG*CMLM NIFAVVKHNNYNYTSISKE*NRTIFIYFCQL*K ERPSAVFPQISPKEKANISHYCYHIPLYKF*KE MEGDLHFFEEISIVTYVNR**QLILLKVPHESPD ALSMPHSNVASFMGFFFFFLADLILSMLYFSLK ARYLSTADVIERK*NSVSRVNPVSCVCSSKVRN DSNLNKKILNIQKLNSN*PQNHLFLCQNLQRVEW TKLCMED*TTVNR*YPNLIWLGLQF*KSVYLGS AQHEISGPCSFPV*PTVL*LRVEQSTFLVVYDI ALFVFPFSTVFSADFFLQIIQPPNANE*YKNK*G TWQRVVLPSLTMWEFDIG*ESEYRFKR*NL*LI ILYLFILDVCI*GKKSGIFAFQ*RGSK*WFFSQ FSKLVYVIALAVWKCALKYAYLLNDHGYMLLRYL KLTLFSLVHCE*TLYYFFKTFTLRVDIIATYIL PELDQRSFV*MSN*KIFKSHYNSIIGEHLLNFF LF*RGKEKPVYLGSLFDPIV*PDSWSNKLSVWLF SECLNFTCLAFHSCTPWSTGAFFHIVVLVKTFC YFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVTNWLVKS*ELLTYFS C*VLRKKRRLEQQIVDLKVTSILERCQTKRKLTN FRKNFCTLS*SIRESWND*KRRTKN*EN*YCRK MTKAFIIESLRNL*LTCILKFLMFSLIMMPVII RKIICHGLLWLEIRVLERLVCWK*LPKLEYSQED LGR**HVLQLR*L*VKVLTMWPYLKIVLGSLIL PKKKILQH*DMK*NFE*GKM*KKAVPLALRPYP *M*KALDYRGWCLLTYQV*LIL*HQAWLLTQRKL FSVSAKLTCRILMPSYCVFKMDLWMLNAVLLQT WSVKWTLMEGEPYSF*PK*TWQRKM*PVQAGFS R*LKESSSQ*KL*VILLL*QEKGTALKALKL*EN MKKSFFRIQSS*RQAC*RHTK*LQEI*ALQYQT AFGKWYESLLNNRLIVSKQHVLTLKLNGRITIL ACGNLTGMNYLKKLKMKSLMKLSV*ARLHQNIGR KSFNNLCGKEYQLM*LKTSTFQLRRP*IQELLT PQWISSLNSGLINNFLIKQ*RLLGRPYKKNFPA L*QNRKGKSMMTYLINLKRLLRKKVLNDTSGMTL RRTA*GLFNTMLWKTDPYLINSNGMQLFILWKR LCRLVSRILKMQLKTWWVQTGKRGGYTGRIGPK NSVFTMKPRMNWRRC*NVMRSTQLILQVMK*PQS GRTLNPEE*K*IQA*LRILGIKFIEDIF*KQL* TIVTFVEEVFITTKGIL*ILSWNAMMWSCFGVY SACLLSPQIL*GNNLQILKLGD*RKMLKRYWKIL LKMVRRRLNCLLVNAFNWRKTSRKLEKFKKNLM LSLKLFIRRNKLKSYS*SALHTSEEQKHQRLLS SLFFFCCSTFLNIQ*SHGIKIIDVCYGRFNHQLP LEWKNSGNGLTSYFVVLK*QIGII*LVWPLGSV LEDKKLVLCLLSYLWWHSFNGLTEVAQCSVSFP EIQC*VF*NKTYIAIV*SYQLYIKSQ*PAKSLYV SVVFYSQKLFDHDNSVYIHHMKEKWVTEEPLKQ VNLDCNVQ*KKFQPFIASEEICLGSQVSTSLPI *FSCCFLTLYIHLKLIYFRCCILTLGFLLIVVFL LLTEPPDYDTG*GRLRIIN*LISFRILSNISTR YQKKAFFHKTILNRNYFNN*SNVDHPPLS*IKK NLVQFIAI*LQYYLHNIFMCFK*IFFNWLKDIQA KKCFLYLKCLSHLLPFH*AFTLLIKVSIV*CF* FYSLLNLIFLELLFGNSPIATPHFIVLHQCMLR CDPSRCNTWYARLRI*KNTSETRNSMYVCFILFD KKSRSSLKQHRCAGRCIVRNFFYFF*V*GQE*I EREESSIMFYTQIRR*PYWVHPSNQCLQVNAC* *IFLQL*SITITTTHLFLKNKTGPYLFTSVNYRK KDLQLYFHRFLPRKRLILVTTVITSLCISFKKR WREIFISLRRSVL*RM*IDDNN*YY*KSHMRVL TPSPCPTVMWLLSWVFFSSF*LISS*ACFIFP*K LGIYQLQMLLKENKIQSQE*TLCLVSVVQKSEM ILI*TKRY*IYRS*IRTSHRIICFYVRICKEWS GQSSVWKTEQL*IDDIQT*FG*DFNFKNQCT*AV HSTK*VALAASPFNPLCYSCGWNSQPF**FMIL PSLYSHFLQFFPQTSFCKLFSLQMQMNDIKISR EHGREWCFPASQCGNLT*DESQSIGLKDKIFS** FCIYLF*MYVSEERNLVFLLSNKGDQSNGFSLS SLSWSML*L*QYGNVL*NMLTF*MIMAICC*DI *NLPCFHLCTVNELCIIFLKPSHYV*ILLQLIFC LSLIKGHLCR*VIKKYLNHIIILLLESIF*IFF CFNEGKRNLYT*GHYLTP*YNQIHGLTSSQCGF SLNA*ISHALHFTVVLHGQPVLFFTSWYLSKHFV IFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRSQIG**SHRSF*PTSL VRFSGRNGV*SNRSWI*K*QAF*KGVRQREN*P TSGRTSAHSVEVSENLGTIRKGEQRIEKISIAER *QRHSS*KA*EIFD*HVF*SS*CSL*L*CQL*Y ARSSATGCCGWRSECWKD*CVGNDCPSSNIPKR IWGDDDTFSS*GDSE*RSSPCGPI*R*FSGV*SY QRRRSCSIKT*NRTSNEEKCERRLYR*P*DHIL KCKRPWTTEDGAC*LTRCD*YCDIRHGS*HKGN YFQYQQSLHAES*CHHTVYSRWICGC*TQYCYRL GQSNGPSWKENHIRFDQSRPGREKCSQSKQDSA DN*RKALPNESFRLFCCCNRKREQL*KH*SYKR I*RRVFSEFKAPKDKHAKGTPSDYKKFKPCSIRL LLENGTRVC*TTG**FQSNTF*P*N*MEE*LSS PAGT*PE*TI*KS*K*NP**SYQSEPGYTKTLG GNPSTIFVGKSINSCD*KHLPSSCADHEFRNF*H HSGYQA*TVD**TTS**SSRGCLGDPTRRIFPL YDRTERERA**HI**T*RGC*GRKY*TTQVE*L CGGQLEGYSTQCFGRPIHI**TAMGCSYLFYGRG SAGSSQGY*KCN*KHGGSRLEKEVVILEESDPR TVCSQ*NQE*IGEDVEM**GAPSLSCK**NNHS PEEP*IPRSRSRSKLD*GYLASSL*KTFFKNSSK PL*PLSKRFLLLPKAFCRF*VGMQ*CGLVLAYT AHACYHRKYFKATTYKY*S*AIREKC*RGIGRF C*RW*EED*IAYW*TRSTGGRPQES*RNSRKT*C FH*SSSSGEIN*NRTHNQLCIHLKNKNINVFCP ASFSSAVPPF*TYNKVMG*K*SMYVTGALTISC LSNGRTVVMD*HPILLY*SDKSE*YNWYGH*VQS LKIRNLFSVCCLICGGTRLMD*LRLLNVQFLFQ KYNARCFEIKLI*QLFKVINCI*NHSSLLNHCM CL*YSIPRNYLTMIIQFIFTT*KKNG*QKNP*NR LIWIVTFSERNFNPS*PAKKFALEAKSVPAYLF DSVAVFSLSISI*N*FILDVVYLR*AFC**WFF SC*QSHRIMTQDEED*G*SID*FHLEYYQTFQLG IRKRLSFIRLF*IEIISTIKVMLTIPLSAE*RK I*FNLLQFNYNTTFTTFSCVLNKYFLIG*RTFK QRNAFFT*NVYLICCLFTKPLLC**KCPLCDVFD FTVC*ILFSWSCFLVTAPLLLPILLFYINACFV VIPQDVTLGMLG*GYEKILPKPGIQCMFVLYCL IRKVGPALSSTDALVDA*SGTFFISFRSRDRSE* KGRRALLCSIHRLGDDLTGYTPLTSAYRLMHVN EYFCSCKA*QLQLHIYF*RIKQDHIYLLLSTIE RKTFSCISTDFSQGKG*Y*SLLLSHPFV*VLKRD GGRSSFL*GDQYCNVCE*MITINITKSPT*ES* RPLHAPQ*CGFFHGFFFLLFS*SHPKHALFFLE S*VFINCRCY*KKIKFSLKSKPCVLCL*FKSQK* F*FKQKDTKYTEVKFELATESFVFMSEFAKSGV DKALYGRLNNCK*MISKLNLARTSILKISVPRQ CTARNKWPLQLPRLTHCAIVAGGTVNLSSSL*YC PLCIPIFYSFFRRLLSANYSASKCK*MI*K*VG NMAESGASQPHNVGI*HRMRVRV*V*KIKSLVN NFVFIYSRCMYLRKEIWYFCFPIKGIKVMVFLSV L*AGLCYSSSSMEMCFKICLPFE*SWLYVVEIF ETYLVFTCAL*MNFVLFF*NLHITCRYYCNLYF A*A*SKVICVDE*LKNI*ITL*FYYWRASFKFFS VLTRERETCIPRVII*PHSITRFMV*QALSVAF L*MLEFHMPCISQLYSMVNRCFFSHRGTCQNIL LFSLVKYIKKVF*FHFAA

Sequence OPA1.dAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTTGTTAGGTT CTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATTCTCTTACAACAACA AATTCAAGAGCATGAAGAGGAAGCGCGCAGAGCCGCTGGCCAATATAGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACAAAGAGAAAATTGAC CAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGA AAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAA TACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGA GGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATC TTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATC CTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAA ACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAG ACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCA GCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGA GAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAG ACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCC TCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGG GAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTA ACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCG CTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGAC TTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAG AGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCA AGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACA GTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTC TAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTAT ACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGAT TTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTG ATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGT CCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCT GCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCA GTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTC CAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTA TGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAA CAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTA TTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTT TCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACT AGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGA AAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCA AGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTT TGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTC GTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTT TGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGA ATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTT AATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAG AAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAG AGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCC TGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTG AAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAA ATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGA GTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGC AGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATA TTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTA GGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTA ATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTC AGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATA TTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATT TTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTT TTCTGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCT TTTCTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTT TGTTATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OPA1.u.f.9 (forward primer)
OPA1.u.r.8 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFGHKLVSEVIGASDLLLLLG SPEETAFRATDRGSESDKHFRKGLLGELILLQQQ IQEHEEEARRAAGQYSTSYAQQKRKVSDKEKID QLQEELLHTQLKYQRILERLEKENKELRKLVLQ KDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYN TQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPR GSGEMMTRSPVKVTLSEGPHHVALFKDSSREFD LTKEEDLAALRHEIELRMRKNVKEGCTVSPETIS LNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKE TIFSISKAYMQNPNAIILCIQDGSVDAERSIVT DLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQ QIIEGKLFPMKALGYFAVVTGKGNSSESIEAIR EYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVS DCFWKMVRESVEQQADSFKATRFNLETEWKNNYP RLRELDRNELFEKAKNEILDEVISLSQVTPKHW EEILQQSLWERVSTHVIENIYLPAAQTMNSGTF NTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSR FMTEPKGKEHDDIFDKLKEAVKEESIKRHKWND FAEDSLRVIQHNALEDRSISDKQQWDAAIYFME EALQARLKDTENAIENMVGPDWKKRWLYWKNRTQ EQCVHNETKNELEKMLKCNEEHPAYLASDEITT VRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTA LNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRI QRMLAITANTLRQQLTNTEVRRLEKNVKEVLED FAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKL DAFIEALHQEK*IKIVLIISSAYI*RTKTSTSFV QPLFLLLFHLSKHTIKSWDKNNRCMLRAL*PSA ASRMEEQW*WINILFCCTKVTNRNNIIGMAIRF SP*R*ETCSLFVVLFVVALV*WIN*GCSMFSFFS RNTMLGVLK*NLYSNCLKLSIVYKITVAC*IIV CVCSILFPETI*P**FSLYSPHERKMGNRRTLK TG*FGL*RSVKEISTLHSQRRNLPWKPSQYQLTY LIQLLFSHSLYPFEIDLF*MLYTYVRLSVNSGF SPVDRATGL*HRMRKIKDNQLTNFI*NIIKHFN *VSEKGFLS*DYFK*KLFQQLK*C*PSPSQLNKE KFSSIYCNLITILPSQHFHVF*INIF*LAKGHS SKEMLSLLKMSISFAAFSLSLYFVNKSVHCVMF LILQFAKSYFLGVAFW*QPHCYSPFYCFTSMHAS L*SLKM*HLVCSVEDMKKYFRNQEFNVCLFYTV **EK*VQP*AAQMRW*MHSQELFLFLLGLGTGV NRKGGELYYVLYTD*EMTLLGTPL*PVLTG*CML MNIFAVVKHNNYNYTSISKE*NRTIFIYFCQL* KERPSAVFPQISPKEKANISHYCYHIPLYKF*K EMEGDLHFFEEISIVTYVNR**QLILLKVPHESP DALSMPHSNVASFMGFFFFFLADLILSMLYFSL KARYLSTADVIERK*NSVSRVNPVSCVCSSKVR NDSNLNKKILNIQKLNSN*PQNHLFLCQNLQRVE WTKLCMED*TTVNR*YPNLIWLGLQF*KSVYLG SAQHEISGPCSFPV*PTVL*LRVEQSTFLVVYD IALFVFPFSTVFSADFFLQIIQPPNANE*YKNK* GTWQRVVLPSLTMWEFDIG*ESEYRFKR*NL*L IILYLFILDVCI*GKKSGIFAFQ*RGSK*WFFS QFSKLVYVIALAVWKCALKYAYLLNDHGYMLLRY LKLTLFSLVHCE*TLYYFFKTFTLRVDIIATYI LPELDQRSFV*MSN*KIFKSHYNSIIGEHLLNF FLF*RGKEKPVYLGSLFDPIV*PDSWSNKLSVWL FSECLNFTCLAFHSCTPWSTGAFFHIVVLVKTF CYFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILVTNWLVKS*ELLTYFSC*V LRKKRRLEQQIVDLKVTSILERVCLVSSFSYNN KFKSMKRKRAEPLANIARAMPNRSARCQTKRKLT NFRKNFCTLS*SIRESWND*KRRTKN*EN*YCR KMTKAFIIESLRNL*LTCILKFLMFSLIMMPVI IRKIICHGLLWLEIRVLERLVCWK*LPKLEYSQE DLGR**HVLQLR*L*VKVLTMWPYLKIVLGSLI LPKKKILQH*DMK*NFE*GKM*KKAVPLALRPY P*M*KALDYRGWCLLTYQV*LIL*HQAWLLTQRK LFSVSAKLTCRILMPSYCVFKMDLWMLNAVLLQ TWSVKWTLMEGEPYSF*PK*TWQRKM*PVQAGF SR*LKESSSQ*KL*VILLL*QEKGTALKALKL*E NMKKSFFRIQSS*RQAC*RHTK*LQEI*ALQYQ TAFGKWYESLLNNRLIVSKQHVLTLKLNGRITI LACGNLTGMNYLKKLKMKSLMKLSV*ARLHQNIG RKSFNNLCGKEYQLM*LKTSTFQLRRP*IQELL TPQWISSLNSGLINNFLIKQ*RLLGRPYKKNFP AL*QNRKGKSMMTYLINLKRLLRKKVLNDTSGMT LRRTA*GLFNTMLWKTDPYLINSNGMQLFILWK RLCRLVSRILKMQLKTWWVQTGKRGGYTGRIGP KNSVFTMKPRMNWRRC*NVMRSTQLILQVMK*PQ SGRTLNPEE*K*IQA*LRILGIKFIEDIF*KQL *TIVTFVEEVFITTKGIL*ILSWNAMMWSCFGV YSACLLSPQIL*GNNLQILKLGD*RKMLKRYWKI LLKMVRRRLNCLLVNAFNWRKTSRKLEKFKKNL MLSLKLFIRRNKLKSYS*SALHTSEEQKHQRLL SSLFFFCCSTFLNIQ*SHGIKIIDVCYGRFNHQL PLEWKNSGNGLTSYFVVLK*QIGII*LVWPLGS VLEDKKLVLCLLSYLWWHSFNGLTEVAQCSVSF PEIQC*VF*NKTYIAIV*SYQLYIKSQ*PAKSLY VSVVFYSQKLFDHDNSVYIHHMKEKWVTEEPLK QVNLDCNVQ*KKFQPFIASEEICLGSQVSTSLP I*FSCCFLTLYIHLKLIYFRCCILTLGFLLIVVF LLLTEPPDYDTG*GRLRIIN*LISFRILSNIST RYQKKAFFHKTILNRNYFNN*SNVDHPPLS*IK KNLVQFIAI*LQYYLHNIFMCFK*IFFNWLKDIQ AKKCFLYLKCLSHLLPFH*AFTLLIKVSIV*CF *FYSLLNLIFLELLFGNSPIATPHFIVLHQCML RCDPSRCNTWYARLRI*KNTSETRNSMYVCFILF DKKSRSSLKQHRCAGRCIVRNFFYFF*V*GQE* IEREESSIMFYTQIRR*PYWVHPSNQCLQVNAC **IFLQL*SITITTTHLFLKNKTGPYLFTSVNYR KKDLQLYFHRFLPRKRLILVTTVITSLCISFKK RWREIFISLRRSVL*RM*IDDNN*YY*KSHMRV LTPSPCPTVMWLLSWVFFSSF*LISS*ACFIFP* KLGIYQLQMLLKENKIQSQE*TLCLVSVVQKSE MILI*TKRY*IYRS*IRTSHRIICFYVRICKEW SGQSSVWKTEQL*IDDIQT*FG*DFNFKNQCT*A VHSTK*VALAASPFNPLCYSCGWNSQPF**FMI LPSLYSHFLQFFPQTSFCKLFSLQMQMNDIKIS REHGREWCFPASQCGNLT*DESQSIGLKDKIFS* *FCIYLF*MYVSEERNLVFLLSNKGDQSNGFSL SSLSWSML*L*QYGNVL*NMLTF*MIMAICC*D I*NLPCFHLCTVNELCIIFLKPSHYV*ILLQLIF CLSLIKGHLCR*VIKKYLNHIIILLLESIF*IF FCFNEGKRNLYT*GHYLTP*YNQIHGLTSSQCG FSLNA*ISHALHFTVVLHGQPVLFFTSWYLSKHF VIFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRFWSQIG**SHRSF*PTSLVRF SGRNGV*SNRSWI*K*QAF*KGSAW*AHSLTTT NSRA*RGSAQSRWPI*HELCPTEAQGVRQREN* PTSGRTSAHSVEVSENLGTIRKGEQRIEKISIAE R*QRHSS*KA*EIFD*HVF*SS*CSL*L*CQL* YARSSATGCCGWRSECWKD*CVGNDCPSSNIPK RIWGDDDTFSS*GDSE*RSSPCGPI*R*FSGV*S YQRRRSCSIKT*NRTSNEEKCERRLYR*P*DHI LKCKRPWTTEDGAC*LTRCD*YCDIRHGS*HKG NYFQYQQSLHAES*CHHTVYSRWICGC*TQYCYR LGQSNGPSWKENHIRFDQSRPGREKCSQSKQDS ADN*RKALPNESFRLFCCCNRKREQL*KH*SYK RI*RRVFSEFKAPKDKHAKGTPSDYKKFKPCSIR LLLENGTRVC*TTG**FQSNTF*P*N*MEE*LS SPAGT*PE*TI*KS*K*NP**SYQSEPGYTKTL GGNPSTIFVGKSINSCD*KHLPSSCADHEFRNF* HHSGYQA*TVD**TTS**SSRGCLGDPTRRIFP LYDRTERERA**HI**T*RGC*GRKY*TTQVE* LCGGQLEGYSTQCFGRPIHI**TAMGCSYLFYGR GSAGSSQGY*KCN*KHGGSRLEKEVVILEESDP RTVCSQ*NQE*IGEDVEM**GAPSLSCK**NNH SPEEP*IPRSRSRSKLD*GYLASSL*KTFFKNSS KPL*PLSKRFLLLPKAFCRF*VGMQ*CGLVLAY TAHACYHRKYFKATTYKY*S*AIREKC*RGIGR FC*RW*EED*IAYW*TRSTGGRPQES*RNSRKT* CFH*SSSSGEIN*NRTHNQLCIHLKNKNINVFC PASFSSAVPPF*TYNKVMG*K*SMYVTGALTIS CLSNGRTVVMD*HPILLY*SDKSE*YNWYGH*VQ SLKIRNLFSVCCLICGGTRLMD*LRLLNVQFLF QKYNARCFEIKLI*QLFKVINCI*NHSSLLNHC MCL*YSIPRNYLTMIIQFIFTT*KKNG*QKNP*N RLIWIVTFSERNFNPS*PAKKFALEAKSVPAYL FDSVAVFSLSISI*N*FILDVVYLR*AFC**WF FSC*QSHRIMTQDEED*G*SID*FHLEYYQTFQL GIRKRLSFIRLF*IEIISTIKVMLTIPLSAE*R KI*FNLLQFNYNTTFTTFSCVLNKYFLIG*RTF KQRNAFFT*NVYLICCLFTKPLLC**KCPLCDVF DFTVC*ILFSWSCFLVTAPLLLPILLFYINACF VVIPQDVTLGMLG*GYEKILPKPGIQCMFVLYC LIRKVGPALSSTDALVDA*SGTFFISFRSRDRSE *KGRRALLCSIHRLGDDLTGYTPLTSAYRLMHV NEYFCSCKA*QLQLHIYF*RIKQDHIYLLLSTI ERKTFSCISTDFSQGKG*Y*SLLLSHPFV*VLKR DGGRSSFL*GDQYCNVCE*MITINITKSPT*ES *RPLHAPQ*CGFFHGFFFLLFS*SHPKHALFFL ES*VFINCRCY*KKIKFSLKSKPCVLCL*FKSQK *F*FKQKDTKYTEVKFELATESFVFMSEFAKSG VDKALYGRLNNCK*MISKLNLARTSILKISVPR QCTARNKWPLQLPRLTHCAIVAGGTVNLSSSL*Y CPLCIPIFYSFFRRLLSANYSASKCK*MI*K*V GNMAESGASQPHNVGI*HRMRVRV*V*KIKSLV NNFVFIYSRCMYLRKEIWYFCFPIKGIKVMVFLS VL*AGLCYSSSSMEMCFKICLPFE*SWLYVVEI FETYLVFTCAL*MNFVLFF*NLHITCRYYCNLY FA*A*SKVICVDE*LKNI*ITL*FYYWRASFKFF SVLTRERETCIPRVII*PHSITRFMV*QALSVA FL*MLEFHMPCISQLYSMVNRCFFSHRGTCQNI LLFSLVKYIKKVF*FHFAA

Sequence OPA1.bAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTT GTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATTCTCTTA CAACAACAAATTCAAGAGCATGAAGAGGAAGCGCGCAGAGCCGCTGGCCAATATAGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACAAAGAGA AAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGT ATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCC AGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATAT TCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGA GTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAG ACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACA CAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTAT TGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGC AGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAG CTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGC AGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAAT AACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAA AACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGG AACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAA TTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGT GGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTT TATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAAT CGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAA TAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAA AACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTT TGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTAT TGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGA AAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACG TCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAAC CATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCAT TAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGT TTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAA TCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAAC CCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAG CTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATA GTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACA TTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTG AATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAG GACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGT GATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATG CATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTT ATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGAC AGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAA TGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTC AACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTT TTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACAT GAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATT TTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAA AGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAA AGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGT ACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGT TTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAA AATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATC TTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTT TTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTG TTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAA CTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTT AAATTTTTTTCTGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCA GTGTGGCTTTTCTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCA AAACATTTTGTTATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OPA1.u.f.9 (forward primer)
OPA1.u.r.8 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGHKLVSEVIGASDLLLL LGSPEETAFRATDRGSESDKHFRKGLLGELILL QQQIQEHEEEARRAAGQYSTSYAQQKRKVSDKE KIDQLQEELLHTQLKYQRILERLEKENKELRKLV LQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDA SYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARI FPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSRE FDLTKEEDLAALRHEIELRMRKNVKEGCTVSPE TISLNVKGPGLQRMVLVDLPGVINTVTSGMAPD TKETIFSISKAYMQNPNAIILCIQDGSVDAERSI VTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPS RIQQIIEGKLFPMKALGYFAVVTGKGNSSESIE AIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLA VSDCFWKMVRESVEQQADSFKATRFNLETEWKN NYPRLRELDRNELFEKAKNEILDEVISLSQVTP KHWEEILQQSLWERVSTHVIENIYLPAAQTMNSG TFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEE FSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHK WNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYF MEEALQARLKDTENAIENMVGPDWKKRWLYWKN RTQEQCVHNETKNELEKMLKCNEEHPAYLASDE ITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLK TALNHCNLCRRGFYYYQRHFVDSELECNDVVLF WRIQRMLAITANTLRQQLTNTEVRRLEKNVKEV LEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQE KLDAFIEALHQEK*IKIVLIISSAYI*RTKTST SFVQPLFLLLFHLSKHTIKSWDKNNRCMLRAL* PSAASRMEEQW*WINILFCCTKVTNRNNIIGMAI RFSP*R*ETCSLFVVLFVVALV*WIN*GCSMFS FFSRNTMLGVLK*NLYSNCLKLSIVYKITVAC* IIVCVCSILFPETI*P**FSLYSPHERKMGNRRT LKTG*FGL*RSVKEISTLHSQRRNLPWKPSQYQ LTYLIQLLFSHSLYPFEIDLF*MLYTYVRLSVN SGFSPVDRATGL*HRMRKIKDNQLTNFI*NIIKH FN*VSEKGFLS*DYFK*KLFQQLK*C*PSPSQL NKEKFSSIYCNLITILPSQHFHVF*INIF*LAK GHSSKEMLSLLKMSISFAAFSLSLYFVNKSVHCV MFLILQFAKSYFLGVAFW*QPHCYSPFYCFTSM HASL*SLKM*HLVCSVEDMKKYFRNQEFNVCLF YTV**EK*VQP*AAQMRW*MHSQELFLFLLGLGT GVNRKGGELYYVLYTD*EMTLLGTPL*PVLTG* CMLMNIFAVVKHNNYNYTSISKE*NRTIFIYFC QL*KERPSAVFPQISPKEKANISHYCYHIPLYKF *KEMEGDLHFFEEISIVTYVNR**QLILLKVPH ESPDALSMPHSNVASFMGFFFFFLADLILSMLY FSLKARYLSTADVIERK*NSVSRVNPVSCVCSSK VRNDSNLNKKILNIQKLNSN*PQNHLFLCQNLQ RVEWTKLCMED*TTVNR*YPNLIWLGLQF*KSV YLGSAQHEISGPCSFPV*PTVL*LRVEQSTFLVV YDIALFVFPFSTVFSADFFLQIIQPPNANE*YK NK*GTWQRVVLPSLTMWEFDIG*ESEYRFKR*N L*LIILYLFILDVCI*GKKSGIFAFQ*RGSK*WF FSQFSKLVYVIALAVWKCALKYAYLLNDHGYML LRYLKLTLFSLVHCE*TLYYFFKTFTLRVDIIA TYILPELDQRSFV*MSN*KIFKSHYNSIIGEHLL NFFLF*RGKEKPVYLGSLFDPIV*PDSWSNKLS VWLFSECLNFTCLAFHSCTPWSTGAFFHIVVLV KTFCYFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVTNWLVKS*ELLTYFS C*VLRKKRRLEQQIVDLKVTSILERVCLVSSFSY NNKFKSMKRKRAEPLANIARAMPNRSARCQTKR KLTNFRKNFCTLS*SIRESWND*KRRTKN*EN* YCRKMTKAFIIESLRNL*LTCILKFLMFSLIMMP VIIRKIICHGLLWLEIRVLERLVCWK*LPKLEY SQEDLGR**HVLQLR*L*VKVLTMWPYLKIVLG SLILPKKKILQH*DMK*NFE*GKM*KKAVPLALR PYP*M*KALDYRGWCLLTYQV*LIL*HQAWLLT QRKLFSVSAKLTCRILMPSYCVFKMDLWMLNAV LLQTWSVKWTLMEGEPYSF*PK*TWQRKM*PVQA GFSR*LKESSSQ*KL*VILLL*QEKGTALKALK L*ENMKKSFFRIQSS*RQAC*RHTK*LQEI*AL QYQTAFGKWYESLLNNRLIVSKQHVLTLKLNGRI TILACGNLTGMNYLKKLKMKSLMKLSV*ARLHQ NIGRKSFNNLCGKEYQLM*LKTSTFQLRRP*IQ ELLTPQWISSLNSGLINNFLIKQ*RLLGRPYKKN FPAL*QNRKGKSMMTYLINLKRLLRKKVLNDTS GMTLRRTA*GLFNTMLWKTDPYLINSNGMQLFI LWKRLCRLVSRILKMQLKTWWVQTGKRGGYTGRI GPKNSVFTMKPRMNWRRC*NVMRSTQLILQVMK *PQSGRTLNPEE*K*IQA*LRILGIKFIEDIF* KQL*TIVTFVEEVFITTKGIL*ILSWNAMMWSCF GVYSACLLSPQIL*GNNLQILKLGD*RKMLKRY WKILLKMVRRRLNCLLVNAFNWRKTSRKLEKFK KNLMLSLKLFIRRNKLKSYS*SALHTSEEQKHQR LLSSLFFFCCSTFLNIQ*SHGIKIIDVCYGRFN HQLPLEWKNSGNGLTSYFVVLK*QIGII*LVWP LGSVLEDKKLVLCLLSYLWWHSFNGLTEVAQCSV SFPEIQC*VF*NKTYIAIV*SYQLYIKSQ*PAK SLYVSVVFYSQKLFDHDNSVYIHHMKEKWVTEE PLKQVNLDCNVQ*KKFQPFIASEEICLGSQVSTS LPI*FSCCFLTLYIHLKLIYFRCCILTLGFLLI VVFLLLTEPPDYDTG*GRLRIIN*LISFRILSN ISTRYQKKAFFHKTILNRNYFNN*SNVDHPPLS* IKKNLVQFIAI*LQYYLHNIFMCFK*IFFNWLK DIQAKKCFLYLKCLSHLLPFH*AFTLLIKVSIV *CF*FYSLLNLIFLELLFGNSPIATPHFIVLHQC MLRCDPSRCNTWYARLRI*KNTSETRNSMYVCF ILFDKKSRSSLKQHRCAGRCIVRNFFYFF*V*G QE*IEREESSIMFYTQIRR*PYWVHPSNQCLQVN AC**IFLQL*SITITTTHLFLKNKTGPYLFTSV NYRKKDLQLYFHRFLPRKRLILVTTVITSLCIS FKKRWREIFISLRRSVL*RM*IDDNN*YY*KSHM RVLTPSPCPTVMWLLSWVFFSSF*LISS*ACFI FP*KLGIYQLQMLLKENKIQSQE*TLCLVSVVQ KSEMILI*TKRY*IYRS*IRTSHRIICFYVRICK EWSGQSSVWKTEQL*IDDIQT*FG*DFNFKNQC T*AVHSTK*VALAASPFNPLCYSCGWNSQPF** FMILPSLYSHFLQFFPQTSFCKLFSLQMQMNDIK ISREHGREWCFPASQCGNLT*DESQSIGLKDKI FS**FCIYLF*MYVSEERNLVFLLSNKGDQSNG FSLSSLSWSML*L*QYGNVL*NMLTF*MIMAICC *DI*NLPCFHLCTVNELCIIFLKPSHYV*ILLQ LIFCLSLIKGHLCR*VIKKYLNHIIILLLESIF *IFFCFNEGKRNLYT*GHYLTP*YNQIHGLTSSQ CGFSLNA*ISHALHFTVVLHGQPVLFFTSWYLS KHFVIFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRSQIG**SHRSF*PTSL VRFSGRNGV*SNRSWI*K*QAF*KGSAW*AHSL TTTNSRA*RGSAQSRWPI*HELCPTEAQGVRQRE N*PTSGRTSAHSVEVSENLGTIRKGEQRIEKIS IAER*QRHSS*KA*EIFD*HVF*SS*CSL*L*C QL*YARSSATGCCGWRSECWKD*CVGNDCPSSNI PKRIWGDDDTFSS*GDSE*RSSPCGPI*R*FSG V*SYQRRRSCSIKT*NRTSNEEKCERRLYR*P* DHILKCKRPWTTEDGAC*LTRCD*YCDIRHGS*H KGNYFQYQQSLHAES*CHHTVYSRWICGC*TQY CYRLGQSNGPSWKENHIRFDQSRPGREKCSQSK QDSADN*RKALPNESFRLFCCCNRKREQL*KH*S YKRI*RRVFSEFKAPKDKHAKGTPSDYKKFKPC SIRLLLENGTRVC*TTG**FQSNTF*P*N*MEE *LSSPAGT*PE*TI*KS*K*NP**SYQSEPGYTK TLGGNPSTIFVGKSINSCD*KHLPSSCADHEFR NF*HHSGYQA*TVD**TTS**SSRGCLGDPTRR IFPLYDRTERERA**HI**T*RGC*GRKY*TTQV E*LCGGQLEGYSTQCFGRPIHI**TAMGCSYLF YGRGSAGSSQGY*KCN*KHGGSRLEKEVVILEE SDPRTVCSQ*NQE*IGEDVEM**GAPSLSCK**N NHSPEEP*IPRSRSRSKLD*GYLASSL*KTFFK NSSKPL*PLSKRFLLLPKAFCRF*VGMQ*CGLV LAYTAHACYHRKYFKATTYKY*S*AIREKC*RGI GRFC*RW*EED*IAYW*TRSTGGRPQES*RNSR KT*CFH*SSSSGEIN*NRTHNQLCIHLKNKNIN VFCPASFSSAVPPF*TYNKVMG*K*SMYVTGALT ISCLSNGRTVVMD*HPILLY*SDKSE*YNWYGH *VQSLKIRNLFSVCCLICGGTRLMD*LRLLNVQ FLFQKYNARCFEIKLI*QLFKVINCI*NHSSLLN HCMCL*YSIPRNYLTMIIQFIFTT*KKNG*QKN P*NRLIWIVTFSERNFNPS*PAKKFALEAKSVP AYLFDSVAVFSLSISI*N*FILDVVYLR*AFC** WFFSC*QSHRIMTQDEED*G*SID*FHLEYYQT FQLGIRKRLSFIRLF*IEIISTIKVMLTIPLSA E*RKI*FNLLQFNYNTTFTTFSCVLNKYFLIG*R TFKQRNAFFT*NVYLICCLFTKPLLC**KCPLC DVFDFTVC*ILFSWSCFLVTAPLLLPILLFYIN ACFVVIPQDVTLGMLG*GYEKILPKPGIQCMFVL YCLIRKVGPALSSTDALVDA*SGTFFISFRSRD RSE*KGRRALLCSIHRLGDDLTGYTPLTSAYRL MHVNEYFCSCKA*QLQLHIYF*RIKQDHIYLLLS TIERKTFSCISTDFSQGKG*Y*SLLLSHPFV*V LKRDGGRSSFL*GDQYCNVCE*MITINITKSPT *ES*RPLHAPQ*CGFFHGFFFLLFS*SHPKHALF FLES*VFINCRCY*KKIKFSLKSKPCVLCL*FK SQK*F*FKQKDTKYTEVKFELATESFVFMSEFA KSGVDKALYGRLNNCK*MISKLNLARTSILKISV PRQCTARNKWPLQLPRLTHCAIVAGGTVNLSSS L*YCPLCIPIFYSFFRRLLSANYSASKCK*MI* K*VGNMAESGASQPHNVGI*HRMRVRV*V*KIKS LVNNFVFIYSRCMYLRKEIWYFCFPIKGIKVMV FLSVL*AGLCYSSSSMEMCFKICLPFE*SWLYV VEIFETYLVFTCAL*MNFVLFF*NLHITCRYYCN LYFA*A*SKVICVDE*LKNI*ITL*FYYWRASF KFFSVLTRERETCIPRVII*PHSITRFMV*QAL SVAFL*MLEFHMPCISQLYSMVNRCFFSHRGTCQ NILLFSLVKYIKKVF*FHFAA