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Sequences

Sequence OPA1.jAug05

OL001014 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVAWIAERSSSVWSPFLMGIPLE*GLPV FSETQLWNKRKFTTTKTTSGFTKHLSFTSSYLK ASTTPIKDILSAVLFSDKPSFT*TEILSN*IWLP ASQEFLASKISYETLKTSLSHTRIGCWGWLHSQ KDF*SVERYDTGP**I*MDCA*HCVGN**VYRF *EN*KSPS*FRRPCKVSTRL*QDC*KP*LIEGLF YLRFSGRNGV*SNRSWI*K*QAF*K

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPG*QKGAHLFGVHF*WGSH*SEVCQS LVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLK LQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGY QPRRNFWPARLATRLLKLRYLILGSAVGGGYTAK KTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDF EKIRKALPSSEDLVKLAPDFDKIVESLSLLKDF FTSGSPEETAFRATDRGSESDKHFRK

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGLDSRKELICLESIFDGDPTRVRSAS L**NTALE*KEVYHYKNYIWFHEAFIIHIILP*S FNDPN*GHPFSSSLL*QTFLYVN*NSLQLNMAT SLAGIFGQQD*LRDS*NFAISY*DRLLGVATQP KRLLISGKI*YRTLVNINGLCLTLCGKLMSISIL RKLEKPFLVQKTL*S*HQTLTRLLKALAY*RTF LPQVLRKKRRLEQQIVDLKVTSILE

Sequence OPA1.cAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAG TGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGA TTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATT CTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGAC TAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCT CACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGA AAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGT GATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGT ATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAG TAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGT AACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAG GCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAG CAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCT TGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAAC ATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAG CAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGA GGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATA TCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTC CAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAA TGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGAT ACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAG ATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGA AGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTG GCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCA GCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGA TAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAA GTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCG TTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTT ATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATAT TCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGC GAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTA GATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAAT CAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAAC AATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCA TGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTA AGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCC ATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATA CTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGT CAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTAC TGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAA AGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATT AGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATA GATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTT CTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCA AGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGC CACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTT AATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTA TAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAA TTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGG ATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATT TTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAAT ATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTT TAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAA TCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTTTTCTGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCA TAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCTTTTCTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTC AACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTTTGTTATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OL001014 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGSPEETAFRATDRGSES DKHFRKVSDKEKIDQLQEELLHTQLKYQRILER LEKENKELRKLVLQKDDKGIHHRKLKKSLIDMY SEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKT SVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGP HHVALFKDSSREFDLTKEEDLAALRHEIELRMR KNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGV INTVTSGMAPDTKETIFSISKAYMQNPNAIILC IQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTK VDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVV TGKGNSSESIEAIREYEEEFFQNSKLLKTSMLK AHQVTTRNLSLAVSDCFWKMVRESVEQQADSFK ATRFNLETEWKNNYPRLRELDRNELFEKAKNEIL DEVISLSQVTPKHWEEILQQSLWERVSTHVIEN IYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNK AVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKE AVKEESIKRHKWNDFAEDSLRVIQHNALEDRSI SDKQQWDAAIYFMEEALQARLKDTENAIENMVG PDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCN EEHPAYLASDEITTVRKNLESRGVEVDPSLIKD TWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFV DSELECNDVVLFWRIQRMLAITANTLRQQLTNTE VRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQL AEDLKKVREIQEKLDAFIEALHQEK*IKIVLII SSAYI*RTKTSTSFVQPLFLLLFHLSKHTIKSWD KNNRCMLRAL*PSAASRMEEQW*WINILFCCTK VTNRNNIIGMAIRFSP*R*ETCSLFVVLFVVAL V*WIN*GCSMFSFFSRNTMLGVLK*NLYSNCLKL SIVYKITVAC*IIVCVCSILFPETI*P**FSLY SPHERKMGNRRTLKTG*FGL*RSVKEISTLHSQ RRNLPWKPSQYQLTYLIQLLFSHSLYPFEIDLF* MLYTYVRLSVNSGFSPVDRATGL*HRMRKIKDN QLTNFI*NIIKHFN*VSEKGFLS*DYFK*KLFQ QLK*C*PSPSQLNKEKFSSIYCNLITILPSQHFH VF*INIF*LAKGHSSKEMLSLLKMSISFAAFSL SLYFVNKSVHCVMFLILQFAKSYFLGVAFW*QP HCYSPFYCFTSMHASL*SLKM*HLVCSVEDMKKY FRNQEFNVCLFYTV**EK*VQP*AAQMRW*MHS QELFLFLLGLGTGVNRKGGELYYVLYTD*EMTL LGTPL*PVLTG*CMLMNIFAVVKHNNYNYTSISK E*NRTIFIYFCQL*KERPSAVFPQISPKEKANI SHYCYHIPLYKF*KEMEGDLHFFEEISIVTYVN R**QLILLKVPHESPDALSMPHSNVASFMGFFFF FLADLILSMLYFSLKARYLSTADVIERK*NSVS RVNPVSCVCSSKVRNDSNLNKKILNIQKLNSN* PQNHLFLCQNLQRVEWTKLCMED*TTVNR*YPNL IWLGLQF*KSVYLGSAQHEISGPCSFPV*PTVL *LRVEQSTFLVVYDIALFVFPFSTVFSADFFLQ IIQPPNANE*YKNK*GTWQRVVLPSLTMWEFDIG *ESEYRFKR*NL*LIILYLFILDVCI*GKKSGI FAFQ*RGSK*WFFSQFSKLVYVIALAVWKCALK YAYLLNDHGYMLLRYLKLTLFSLVHCE*TLYYFF KTFTLRVDIIATYILPELDQRSFV*MSN*KIFK SHYNSIIGEHLLNFFLF*RGKEKPVYLGSLFDP IV*PDSWSNKLSVWLFSECLNFTCLAFHSCTPWS TGAFFHIVVLVKTFCYFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVLRKKRRLEQQIVDLK VTSILERCQTKRKLTNFRKNFCTLS*SIRESWND *KRRTKN*EN*YCRKMTKAFIIESLRNL*LTCI LKFLMFSLIMMPVIIRKIICHGLLWLEIRVLER LVCWK*LPKLEYSQEDLGR**HVLQLR*L*VKVL TMWPYLKIVLGSLILPKKKILQH*DMK*NFE*G KM*KKAVPLALRPYP*M*KALDYRGWCLLTYQV *LIL*HQAWLLTQRKLFSVSAKLTCRILMPSYCV FKMDLWMLNAVLLQTWSVKWTLMEGEPYSF*PK *TWQRKM*PVQAGFSR*LKESSSQ*KL*VILLL *QEKGTALKALKL*ENMKKSFFRIQSS*RQAC*R HTK*LQEI*ALQYQTAFGKWYESLLNNRLIVSK QHVLTLKLNGRITILACGNLTGMNYLKKLKMKS LMKLSV*ARLHQNIGRKSFNNLCGKEYQLM*LKT STFQLRRP*IQELLTPQWISSLNSGLINNFLIK Q*RLLGRPYKKNFPAL*QNRKGKSMMTYLINLK RLLRKKVLNDTSGMTLRRTA*GLFNTMLWKTDPY LINSNGMQLFILWKRLCRLVSRILKMQLKTWWV QTGKRGGYTGRIGPKNSVFTMKPRMNWRRC*NV MRSTQLILQVMK*PQSGRTLNPEE*K*IQA*LRI LGIKFIEDIF*KQL*TIVTFVEEVFITTKGIL* ILSWNAMMWSCFGVYSACLLSPQIL*GNNLQIL KLGD*RKMLKRYWKILLKMVRRRLNCLLVNAFNW RKTSRKLEKFKKNLMLSLKLFIRRNKLKSYS*S ALHTSEEQKHQRLLSSLFFFCCSTFLNIQ*SHG IKIIDVCYGRFNHQLPLEWKNSGNGLTSYFVVLK *QIGII*LVWPLGSVLEDKKLVLCLLSYLWWHS FNGLTEVAQCSVSFPEIQC*VF*NKTYIAIV*S YQLYIKSQ*PAKSLYVSVVFYSQKLFDHDNSVYI HHMKEKWVTEEPLKQVNLDCNVQ*KKFQPFIAS EEICLGSQVSTSLPI*FSCCFLTLYIHLKLIYF RCCILTLGFLLIVVFLLLTEPPDYDTG*GRLRII N*LISFRILSNISTRYQKKAFFHKTILNRNYFN N*SNVDHPPLS*IKKNLVQFIAI*LQYYLHNIF MCFK*IFFNWLKDIQAKKCFLYLKCLSHLLPFH* AFTLLIKVSIV*CF*FYSLLNLIFLELLFGNSP IATPHFIVLHQCMLRCDPSRCNTWYARLRI*KN TSETRNSMYVCFILFDKKSRSSLKQHRCAGRCIV RNFFYFF*V*GQE*IEREESSIMFYTQIRR*PY WVHPSNQCLQVNAC**IFLQL*SITITTTHLFL KNKTGPYLFTSVNYRKKDLQLYFHRFLPRKRLIL VTTVITSLCISFKKRWREIFISLRRSVL*RM*I DDNN*YY*KSHMRVLTPSPCPTVMWLLSWVFFS SF*LISS*ACFIFP*KLGIYQLQMLLKENKIQSQ E*TLCLVSVVQKSEMILI*TKRY*IYRS*IRTS HRIICFYVRICKEWSGQSSVWKTEQL*IDDIQT *FG*DFNFKNQCT*AVHSTK*VALAASPFNPLCY SCGWNSQPF**FMILPSLYSHFLQFFPQTSFCK LFSLQMQMNDIKISREHGREWCFPASQCGNLT* DESQSIGLKDKIFS**FCIYLF*MYVSEERNLVF LLSNKGDQSNGFSLSSLSWSML*L*QYGNVL*N MLTF*MIMAICC*DI*NLPCFHLCTVNELCIIF LKPSHYV*ILLQLIFCLSLIKGHLCR*VIKKYLN HIIILLLESIF*IFFCFNEGKRNLYT*GHYLTP *YNQIHGLTSSQCGFSLNA*ISHALHFTVVLHG QPVLFFTSWYLSKHFVIFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRFSGRNGV*SNRSWI*K *QAF*KGVRQREN*PTSGRTSAHSVEVSENLGT IRKGEQRIEKISIAER*QRHSS*KA*EIFD*HVF *SS*CSL*L*CQL*YARSSATGCCGWRSECWKD *CVGNDCPSSNIPKRIWGDDDTFSS*GDSE*RS SPCGPI*R*FSGV*SYQRRRSCSIKT*NRTSNEE KCERRLYR*P*DHILKCKRPWTTEDGAC*LTRC D*YCDIRHGS*HKGNYFQYQQSLHAES*CHHTV YSRWICGC*TQYCYRLGQSNGPSWKENHIRFDQS RPGREKCSQSKQDSADN*RKALPNESFRLFCCC NRKREQL*KH*SYKRI*RRVFSEFKAPKDKHAK GTPSDYKKFKPCSIRLLLENGTRVC*TTG**FQS NTF*P*N*MEE*LSSPAGT*PE*TI*KS*K*NP **SYQSEPGYTKTLGGNPSTIFVGKSINSCD*K HLPSSCADHEFRNF*HHSGYQA*TVD**TTS**S SRGCLGDPTRRIFPLYDRTERERA**HI**T*R GC*GRKY*TTQVE*LCGGQLEGYSTQCFGRPIH I**TAMGCSYLFYGRGSAGSSQGY*KCN*KHGGS RLEKEVVILEESDPRTVCSQ*NQE*IGEDVEM* *GAPSLSCK**NNHSPEEP*IPRSRSRSKLD*G YLASSL*KTFFKNSSKPL*PLSKRFLLLPKAFCR F*VGMQ*CGLVLAYTAHACYHRKYFKATTYKY* S*AIREKC*RGIGRFC*RW*EED*IAYW*TRST GGRPQES*RNSRKT*CFH*SSSSGEIN*NRTHNQ LCIHLKNKNINVFCPASFSSAVPPF*TYNKVMG *K*SMYVTGALTISCLSNGRTVVMD*HPILLY* SDKSE*YNWYGH*VQSLKIRNLFSVCCLICGGTR LMD*LRLLNVQFLFQKYNARCFEIKLI*QLFKV INCI*NHSSLLNHCMCL*YSIPRNYLTMIIQFI FTT*KKNG*QKNP*NRLIWIVTFSERNFNPS*PA KKFALEAKSVPAYLFDSVAVFSLSISI*N*FIL DVVYLR*AFC**WFFSC*QSHRIMTQDEED*G* SID*FHLEYYQTFQLGIRKRLSFIRLF*IEIIST IKVMLTIPLSAE*RKI*FNLLQFNYNTTFTTFS CVLNKYFLIG*RTFKQRNAFFT*NVYLICCLFT KPLLC**KCPLCDVFDFTVC*ILFSWSCFLVTAP LLLPILLFYINACFVVIPQDVTLGMLG*GYEKI LPKPGIQCMFVLYCLIRKVGPALSSTDALVDA* SGTFFISFRSRDRSE*KGRRALLCSIHRLGDDLT GYTPLTSAYRLMHVNEYFCSCKA*QLQLHIYF* RIKQDHIYLLLSTIERKTFSCISTDFSQGKG*Y *SLLLSHPFV*VLKRDGGRSSFL*GDQYCNVCE* MITINITKSPT*ES*RPLHAPQ*CGFFHGFFFL LFS*SHPKHALFFLES*VFINCRCY*KKIKFSL KSKPCVLCL*FKSQK*F*FKQKDTKYTEVKFELA TESFVFMSEFAKSGVDKALYGRLNNCK*MISKL NLARTSILKISVPRQCTARNKWPLQLPRLTHCA IVAGGTVNLSSSL*YCPLCIPIFYSFFRRLLSAN YSASKCK*MI*K*VGNMAESGASQPHNVGI*HR MRVRV*V*KIKSLVNNFVFIYSRCMYLRKEIWY FCFPIKGIKVMVFLSVL*AGLCYSSSSMEMCFKI CLPFE*SWLYVVEIFETYLVFTCAL*MNFVLFF *NLHITCRYYCNLYFA*A*SKVICVDE*LKNI* ITL*FYYWRASFKFFSVLTRERETCIPRVII*PH SITRFMV*QALSVAFL*MLEFHMPCISQLYSMV NRCFFSHRGTCQNILLFSLVKYIKKVF*FHFAA

Sequence OPA1.aAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAG TGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATTCTCTTACAACAACAAATTCAAGAGCATGAAGAGGAAGCGCGCAGAGCCGCTGGCCAATAT AGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAA TCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGAT TGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGT GCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGA GTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACT TCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGAC TTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCA TCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGT TTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTAT TTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAA GCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGA TAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAA AATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATG TGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACT TCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGAT AAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAG ACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAA CATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATG TTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCT TGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAG GCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTT ACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAAC GCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGT ACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATA AAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTT GTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGT GGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAAT TGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAAT TCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCT TCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATT GATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGA TTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAA ATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCA CAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTG CCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTG GTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGAT ATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGT AGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGA GATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACA TCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAA AGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAAC GTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGT TTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAA ATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAA TTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGA TATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAAC CCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTT CTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAA TTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGA AATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATG TGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTG TATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAA AAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTTTTCTGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATT ATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCTTTTCTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGT ACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTTTGTTATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCA CTTTGCTGCC

OL001014 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGSPEETAFRATDRGSES DKHFRKGLLGELILLQQQIQEHEEEARRAAGQY STSYAQQKRKVSDKEKIDQLQEELLHTQLKYQR ILERLEKENKELRKLVLQKDDKGIHHRKLKKSLI DMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQS AGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTL SEGPHHVALFKDSSREFDLTKEEDLAALRHEIEL RMRKNVKEGCTVSPETISLNVKGPGLQRMVLVD LPGVINTVTSGMAPDTKETIFSISKAYMQNPNA IILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFV LTKVDLAEKNVASPSRIQQIIEGKLFPMKALGY FAVVTGKGNSSESIEAIREYEEEFFQNSKLLKT SMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQAD SFKATRFNLETEWKNNYPRLRELDRNELFEKAK NEILDEVISLSQVTPKHWEEILQQSLWERVSTH VIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQL PNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFD KLKEAVKEESIKRHKWNDFAEDSLRVIQHNALE DRSISDKQQWDAAIYFMEEALQARLKDTENAIEN MVGPDWKKRWLYWKNRTQEQCVHNETKNELEKM LKCNEEHPAYLASDEITTVRKNLESRGVEVDPS LIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQR HFVDSELECNDVVLFWRIQRMLAITANTLRQQL TNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGK RVQLAEDLKKVREIQEKLDAFIEALHQEK*IKIV LIISSAYI*RTKTSTSFVQPLFLLLFHLSKHTI KSWDKNNRCMLRAL*PSAASRMEEQW*WINILF CCTKVTNRNNIIGMAIRFSP*R*ETCSLFVVLFV VALV*WIN*GCSMFSFFSRNTMLGVLK*NLYSN CLKLSIVYKITVAC*IIVCVCSILFPETI*P** FSLYSPHERKMGNRRTLKTG*FGL*RSVKEISTL HSQRRNLPWKPSQYQLTYLIQLLFSHSLYPFEI DLF*MLYTYVRLSVNSGFSPVDRATGL*HRMRK IKDNQLTNFI*NIIKHFN*VSEKGFLS*DYFK*K LFQQLK*C*PSPSQLNKEKFSSIYCNLITILPS QHFHVF*INIF*LAKGHSSKEMLSLLKMSISFA AFSLSLYFVNKSVHCVMFLILQFAKSYFLGVAFW *QPHCYSPFYCFTSMHASL*SLKM*HLVCSVED MKKYFRNQEFNVCLFYTV**EK*VQP*AAQMRW *MHSQELFLFLLGLGTGVNRKGGELYYVLYTD*E MTLLGTPL*PVLTG*CMLMNIFAVVKHNNYNYT SISKE*NRTIFIYFCQL*KERPSAVFPQISPKE KANISHYCYHIPLYKF*KEMEGDLHFFEEISIVT YVNR**QLILLKVPHESPDALSMPHSNVASFMG FFFFFLADLILSMLYFSLKARYLSTADVIERK* NSVSRVNPVSCVCSSKVRNDSNLNKKILNIQKLN SN*PQNHLFLCQNLQRVEWTKLCMED*TTVNR* YPNLIWLGLQF*KSVYLGSAQHEISGPCSFPV* PTVL*LRVEQSTFLVVYDIALFVFPFSTVFSADF FLQIIQPPNANE*YKNK*GTWQRVVLPSLTMWE FDIG*ESEYRFKR*NL*LIILYLFILDVCI*GK KSGIFAFQ*RGSK*WFFSQFSKLVYVIALAVWKC ALKYAYLLNDHGYMLLRYLKLTLFSLVHCE*TL YYFFKTFTLRVDIIATYILPELDQRSFV*MSN* KIFKSHYNSIIGEHLLNFFLF*RGKEKPVYLGSL FDPIV*PDSWSNKLSVWLFSECLNFTCLAFHSC TPWSTGAFFHIVVLVKTFCYFPW*NI*KRFSNF TLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVLRKKRRLEQQIVDLK VTSILERVCLVSSFSYNNKFKSMKRKRAEPLANI ARAMPNRSARCQTKRKLTNFRKNFCTLS*SIRE SWND*KRRTKN*EN*YCRKMTKAFIIESLRNL* LTCILKFLMFSLIMMPVIIRKIICHGLLWLEIRV LERLVCWK*LPKLEYSQEDLGR**HVLQLR*L* VKVLTMWPYLKIVLGSLILPKKKILQH*DMK*N FE*GKM*KKAVPLALRPYP*M*KALDYRGWCLLT YQV*LIL*HQAWLLTQRKLFSVSAKLTCRILMP SYCVFKMDLWMLNAVLLQTWSVKWTLMEGEPYS F*PK*TWQRKM*PVQAGFSR*LKESSSQ*KL*VI LLL*QEKGTALKALKL*ENMKKSFFRIQSS*RQ AC*RHTK*LQEI*ALQYQTAFGKWYESLLNNRL IVSKQHVLTLKLNGRITILACGNLTGMNYLKKLK MKSLMKLSV*ARLHQNIGRKSFNNLCGKEYQLM *LKTSTFQLRRP*IQELLTPQWISSLNSGLINN FLIKQ*RLLGRPYKKNFPAL*QNRKGKSMMTYLI NLKRLLRKKVLNDTSGMTLRRTA*GLFNTMLWK TDPYLINSNGMQLFILWKRLCRLVSRILKMQLK TWWVQTGKRGGYTGRIGPKNSVFTMKPRMNWRRC *NVMRSTQLILQVMK*PQSGRTLNPEE*K*IQA *LRILGIKFIEDIF*KQL*TIVTFVEEVFITTK GIL*ILSWNAMMWSCFGVYSACLLSPQIL*GNNL QILKLGD*RKMLKRYWKILLKMVRRRLNCLLVN AFNWRKTSRKLEKFKKNLMLSLKLFIRRNKLKS YS*SALHTSEEQKHQRLLSSLFFFCCSTFLNIQ* SHGIKIIDVCYGRFNHQLPLEWKNSGNGLTSYF VVLK*QIGII*LVWPLGSVLEDKKLVLCLLSYL WWHSFNGLTEVAQCSVSFPEIQC*VF*NKTYIAI V*SYQLYIKSQ*PAKSLYVSVVFYSQKLFDHDN SVYIHHMKEKWVTEEPLKQVNLDCNVQ*KKFQP FIASEEICLGSQVSTSLPI*FSCCFLTLYIHLKL IYFRCCILTLGFLLIVVFLLLTEPPDYDTG*GR LRIIN*LISFRILSNISTRYQKKAFFHKTILNR NYFNN*SNVDHPPLS*IKKNLVQFIAI*LQYYLH NIFMCFK*IFFNWLKDIQAKKCFLYLKCLSHLL PFH*AFTLLIKVSIV*CF*FYSLLNLIFLELLF GNSPIATPHFIVLHQCMLRCDPSRCNTWYARLRI *KNTSETRNSMYVCFILFDKKSRSSLKQHRCAG RCIVRNFFYFF*V*GQE*IEREESSIMFYTQIR R*PYWVHPSNQCLQVNAC**IFLQL*SITITTTH LFLKNKTGPYLFTSVNYRKKDLQLYFHRFLPRK RLILVTTVITSLCISFKKRWREIFISLRRSVL* RM*IDDNN*YY*KSHMRVLTPSPCPTVMWLLSWV FFSSF*LISS*ACFIFP*KLGIYQLQMLLKENK IQSQE*TLCLVSVVQKSEMILI*TKRY*IYRS* IRTSHRIICFYVRICKEWSGQSSVWKTEQL*IDD IQT*FG*DFNFKNQCT*AVHSTK*VALAASPFN PLCYSCGWNSQPF**FMILPSLYSHFLQFFPQT SFCKLFSLQMQMNDIKISREHGREWCFPASQCGN LT*DESQSIGLKDKIFS**FCIYLF*MYVSEER NLVFLLSNKGDQSNGFSLSSLSWSML*L*QYGN VL*NMLTF*MIMAICC*DI*NLPCFHLCTVNELC IIFLKPSHYV*ILLQLIFCLSLIKGHLCR*VIK KYLNHIIILLLESIF*IFFCFNEGKRNLYT*GH YLTP*YNQIHGLTSSQCGFSLNA*ISHALHFTVV LHGQPVLFFTSWYLSKHFVIFLGKIYKKGFLIS LCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRFSGRNGV*SNRSWI*K *QAF*KGSAW*AHSLTTTNSRA*RGSAQSRWPI *HELCPTEAQGVRQREN*PTSGRTSAHSVEVSEN LGTIRKGEQRIEKISIAER*QRHSS*KA*EIFD *HVF*SS*CSL*L*CQL*YARSSATGCCGWRSE CWKD*CVGNDCPSSNIPKRIWGDDDTFSS*GDSE *RSSPCGPI*R*FSGV*SYQRRRSCSIKT*NRT SNEEKCERRLYR*P*DHILKCKRPWTTEDGAC* LTRCD*YCDIRHGS*HKGNYFQYQQSLHAES*CH HTVYSRWICGC*TQYCYRLGQSNGPSWKENHIR FDQSRPGREKCSQSKQDSADN*RKALPNESFRL FCCCNRKREQL*KH*SYKRI*RRVFSEFKAPKDK HAKGTPSDYKKFKPCSIRLLLENGTRVC*TTG* *FQSNTF*P*N*MEE*LSSPAGT*PE*TI*KS* K*NP**SYQSEPGYTKTLGGNPSTIFVGKSINSC D*KHLPSSCADHEFRNF*HHSGYQA*TVD**TT S**SSRGCLGDPTRRIFPLYDRTERERA**HI* *T*RGC*GRKY*TTQVE*LCGGQLEGYSTQCFGR PIHI**TAMGCSYLFYGRGSAGSSQGY*KCN*K HGGSRLEKEVVILEESDPRTVCSQ*NQE*IGED VEM**GAPSLSCK**NNHSPEEP*IPRSRSRSKL D*GYLASSL*KTFFKNSSKPL*PLSKRFLLLPK AFCRF*VGMQ*CGLVLAYTAHACYHRKYFKATT YKY*S*AIREKC*RGIGRFC*RW*EED*IAYW*T RSTGGRPQES*RNSRKT*CFH*SSSSGEIN*NR THNQLCIHLKNKNINVFCPASFSSAVPPF*TYN KVMG*K*SMYVTGALTISCLSNGRTVVMD*HPIL LY*SDKSE*YNWYGH*VQSLKIRNLFSVCCLIC GGTRLMD*LRLLNVQFLFQKYNARCFEIKLI*Q LFKVINCI*NHSSLLNHCMCL*YSIPRNYLTMII QFIFTT*KKNG*QKNP*NRLIWIVTFSERNFNP S*PAKKFALEAKSVPAYLFDSVAVFSLSISI*N *FILDVVYLR*AFC**WFFSC*QSHRIMTQDEED *G*SID*FHLEYYQTFQLGIRKRLSFIRLF*IE IISTIKVMLTIPLSAE*RKI*FNLLQFNYNTTF TTFSCVLNKYFLIG*RTFKQRNAFFT*NVYLICC LFTKPLLC**KCPLCDVFDFTVC*ILFSWSCFL VTAPLLLPILLFYINACFVVIPQDVTLGMLG*G YEKILPKPGIQCMFVLYCLIRKVGPALSSTDALV DA*SGTFFISFRSRDRSE*KGRRALLCSIHRLG DDLTGYTPLTSAYRLMHVNEYFCSCKA*QLQLH IYF*RIKQDHIYLLLSTIERKTFSCISTDFSQGK G*Y*SLLLSHPFV*VLKRDGGRSSFL*GDQYCN VCE*MITINITKSPT*ES*RPLHAPQ*CGFFHG FFFLLFS*SHPKHALFFLES*VFINCRCY*KKIK FSLKSKPCVLCL*FKSQK*F*FKQKDTKYTEVK FELATESFVFMSEFAKSGVDKALYGRLNNCK*M ISKLNLARTSILKISVPRQCTARNKWPLQLPRLT HCAIVAGGTVNLSSSL*YCPLCIPIFYSFFRRL LSANYSASKCK*MI*K*VGNMAESGASQPHNVG I*HRMRVRV*V*KIKSLVNNFVFIYSRCMYLRKE IWYFCFPIKGIKVMVFLSVL*AGLCYSSSSMEM CFKICLPFE*SWLYVVEIFETYLVFTCAL*MNF VLFF*NLHITCRYYCNLYFA*A*SKVICVDE*LK NI*ITL*FYYWRASFKFFSVLTRERETCIPRVI I*PHSITRFMV*QALSVAFL*MLEFHMPCISQL YSMVNRCFFSHRGTCQNILLFSLVKYIKKVF*FH FAA

Sequence OPA1.eAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTT GTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAA CTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAG ATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATAC GCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGA TCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTA CCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTT AAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACT ATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACT TGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCA GATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAA TATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACT GCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCG CCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAG GAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACA CCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTT TATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTT GCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGG CTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGA ACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTC CGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAA ACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACA GCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTT GCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATG CTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCA GCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCC TCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTC CTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAG AAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGT GTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAG GTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTT GATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCT CCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGG TATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAA ATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGC AAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGA TTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTT GTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGA TAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATA GAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAAT GAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAA GAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGA TGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGA CGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAA GCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATG ATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTG GACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGT GCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTG CCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGG AACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATA ATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGT TCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTT GAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTG CCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTTAAATTTTTTTC TGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCAGTGTGGCTTTT CTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCAAAACATTTTGT TATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OL001014 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGHKLVSEVIGASDLLLL LGSPEETAFRATDRGSESDKHFRKVSDKEKIDQ LQEELLHTQLKYQRILERLEKENKELRKLVLQK DDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNT QDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRG SGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISL NVKGPGLQRMVLVDLPGVINTVTSGMAPDTKET IFSISKAYMQNPNAIILCIQDGSVDAERSIVTD LVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQ IIEGKLFPMKALGYFAVVTGKGNSSESIEAIRE YEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSD CFWKMVRESVEQQADSFKATRFNLETEWKNNYPR LRELDRNELFEKAKNEILDEVISLSQVTPKHWE EILQQSLWERVSTHVIENIYLPAAQTMNSGTFN TTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRF MTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDF AEDSLRVIQHNALEDRSISDKQQWDAAIYFMEE ALQARLKDTENAIENMVGPDWKKRWLYWKNRTQE QCVHNETKNELEKMLKCNEEHPAYLASDEITTV RKNLESRGVEVDPSLIKDTWHQVYRRHFLKTAL NHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQ RMLAITANTLRQQLTNTEVRRLEKNVKEVLEDF AEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLD AFIEALHQEK*IKIVLIISSAYI*RTKTSTSFVQ PLFLLLFHLSKHTIKSWDKNNRCMLRAL*PSAA SRMEEQW*WINILFCCTKVTNRNNIIGMAIRFS P*R*ETCSLFVVLFVVALV*WIN*GCSMFSFFSR NTMLGVLK*NLYSNCLKLSIVYKITVAC*IIVC VCSILFPETI*P**FSLYSPHERKMGNRRTLKT G*FGL*RSVKEISTLHSQRRNLPWKPSQYQLTYL IQLLFSHSLYPFEIDLF*MLYTYVRLSVNSGFS PVDRATGL*HRMRKIKDNQLTNFI*NIIKHFN* VSEKGFLS*DYFK*KLFQQLK*C*PSPSQLNKEK FSSIYCNLITILPSQHFHVF*INIF*LAKGHSS KEMLSLLKMSISFAAFSLSLYFVNKSVHCVMFL ILQFAKSYFLGVAFW*QPHCYSPFYCFTSMHASL *SLKM*HLVCSVEDMKKYFRNQEFNVCLFYTV* *EK*VQP*AAQMRW*MHSQELFLFLLGLGTGVN RKGGELYYVLYTD*EMTLLGTPL*PVLTG*CMLM NIFAVVKHNNYNYTSISKE*NRTIFIYFCQL*K ERPSAVFPQISPKEKANISHYCYHIPLYKF*KE MEGDLHFFEEISIVTYVNR**QLILLKVPHESPD ALSMPHSNVASFMGFFFFFLADLILSMLYFSLK ARYLSTADVIERK*NSVSRVNPVSCVCSSKVRN DSNLNKKILNIQKLNSN*PQNHLFLCQNLQRVEW TKLCMED*TTVNR*YPNLIWLGLQF*KSVYLGS AQHEISGPCSFPV*PTVL*LRVEQSTFLVVYDI ALFVFPFSTVFSADFFLQIIQPPNANE*YKNK*G TWQRVVLPSLTMWEFDIG*ESEYRFKR*NL*LI ILYLFILDVCI*GKKSGIFAFQ*RGSK*WFFSQ FSKLVYVIALAVWKCALKYAYLLNDHGYMLLRYL KLTLFSLVHCE*TLYYFFKTFTLRVDIIATYIL PELDQRSFV*MSN*KIFKSHYNSIIGEHLLNFF LF*RGKEKPVYLGSLFDPIV*PDSWSNKLSVWLF SECLNFTCLAFHSCTPWSTGAFFHIVVLVKTFC YFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVTNWLVKS*ELLTYFS C*VLRKKRRLEQQIVDLKVTSILERCQTKRKLTN FRKNFCTLS*SIRESWND*KRRTKN*EN*YCRK MTKAFIIESLRNL*LTCILKFLMFSLIMMPVII RKIICHGLLWLEIRVLERLVCWK*LPKLEYSQED LGR**HVLQLR*L*VKVLTMWPYLKIVLGSLIL PKKKILQH*DMK*NFE*GKM*KKAVPLALRPYP *M*KALDYRGWCLLTYQV*LIL*HQAWLLTQRKL FSVSAKLTCRILMPSYCVFKMDLWMLNAVLLQT WSVKWTLMEGEPYSF*PK*TWQRKM*PVQAGFS R*LKESSSQ*KL*VILLL*QEKGTALKALKL*EN MKKSFFRIQSS*RQAC*RHTK*LQEI*ALQYQT AFGKWYESLLNNRLIVSKQHVLTLKLNGRITIL ACGNLTGMNYLKKLKMKSLMKLSV*ARLHQNIGR KSFNNLCGKEYQLM*LKTSTFQLRRP*IQELLT PQWISSLNSGLINNFLIKQ*RLLGRPYKKNFPA L*QNRKGKSMMTYLINLKRLLRKKVLNDTSGMTL RRTA*GLFNTMLWKTDPYLINSNGMQLFILWKR LCRLVSRILKMQLKTWWVQTGKRGGYTGRIGPK NSVFTMKPRMNWRRC*NVMRSTQLILQVMK*PQS GRTLNPEE*K*IQA*LRILGIKFIEDIF*KQL* TIVTFVEEVFITTKGIL*ILSWNAMMWSCFGVY SACLLSPQIL*GNNLQILKLGD*RKMLKRYWKIL LKMVRRRLNCLLVNAFNWRKTSRKLEKFKKNLM LSLKLFIRRNKLKSYS*SALHTSEEQKHQRLLS SLFFFCCSTFLNIQ*SHGIKIIDVCYGRFNHQLP LEWKNSGNGLTSYFVVLK*QIGII*LVWPLGSV LEDKKLVLCLLSYLWWHSFNGLTEVAQCSVSFP EIQC*VF*NKTYIAIV*SYQLYIKSQ*PAKSLYV SVVFYSQKLFDHDNSVYIHHMKEKWVTEEPLKQ VNLDCNVQ*KKFQPFIASEEICLGSQVSTSLPI *FSCCFLTLYIHLKLIYFRCCILTLGFLLIVVFL LLTEPPDYDTG*GRLRIIN*LISFRILSNISTR YQKKAFFHKTILNRNYFNN*SNVDHPPLS*IKK NLVQFIAI*LQYYLHNIFMCFK*IFFNWLKDIQA KKCFLYLKCLSHLLPFH*AFTLLIKVSIV*CF* FYSLLNLIFLELLFGNSPIATPHFIVLHQCMLR CDPSRCNTWYARLRI*KNTSETRNSMYVCFILFD KKSRSSLKQHRCAGRCIVRNFFYFF*V*GQE*I EREESSIMFYTQIRR*PYWVHPSNQCLQVNAC* *IFLQL*SITITTTHLFLKNKTGPYLFTSVNYRK KDLQLYFHRFLPRKRLILVTTVITSLCISFKKR WREIFISLRRSVL*RM*IDDNN*YY*KSHMRVL TPSPCPTVMWLLSWVFFSSF*LISS*ACFIFP*K LGIYQLQMLLKENKIQSQE*TLCLVSVVQKSEM ILI*TKRY*IYRS*IRTSHRIICFYVRICKEWS GQSSVWKTEQL*IDDIQT*FG*DFNFKNQCT*AV HSTK*VALAASPFNPLCYSCGWNSQPF**FMIL PSLYSHFLQFFPQTSFCKLFSLQMQMNDIKISR EHGREWCFPASQCGNLT*DESQSIGLKDKIFS** FCIYLF*MYVSEERNLVFLLSNKGDQSNGFSLS SLSWSML*L*QYGNVL*NMLTF*MIMAICC*DI *NLPCFHLCTVNELCIIFLKPSHYV*ILLQLIFC LSLIKGHLCR*VIKKYLNHIIILLLESIF*IFF CFNEGKRNLYT*GHYLTP*YNQIHGLTSSQCGF SLNA*ISHALHFTVVLHGQPVLFFTSWYLSKHFV IFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRSQIG**SHRSF*PTSL VRFSGRNGV*SNRSWI*K*QAF*KGVRQREN*P TSGRTSAHSVEVSENLGTIRKGEQRIEKISIAER *QRHSS*KA*EIFD*HVF*SS*CSL*L*CQL*Y ARSSATGCCGWRSECWKD*CVGNDCPSSNIPKR IWGDDDTFSS*GDSE*RSSPCGPI*R*FSGV*SY QRRRSCSIKT*NRTSNEEKCERRLYR*P*DHIL KCKRPWTTEDGAC*LTRCD*YCDIRHGS*HKGN YFQYQQSLHAES*CHHTVYSRWICGC*TQYCYRL GQSNGPSWKENHIRFDQSRPGREKCSQSKQDSA DN*RKALPNESFRLFCCCNRKREQL*KH*SYKR I*RRVFSEFKAPKDKHAKGTPSDYKKFKPCSIRL LLENGTRVC*TTG**FQSNTF*P*N*MEE*LSS PAGT*PE*TI*KS*K*NP**SYQSEPGYTKTLG GNPSTIFVGKSINSCD*KHLPSSCADHEFRNF*H HSGYQA*TVD**TTS**SSRGCLGDPTRRIFPL YDRTERERA**HI**T*RGC*GRKY*TTQVE*L CGGQLEGYSTQCFGRPIHI**TAMGCSYLFYGRG SAGSSQGY*KCN*KHGGSRLEKEVVILEESDPR TVCSQ*NQE*IGEDVEM**GAPSLSCK**NNHS PEEP*IPRSRSRSKLD*GYLASSL*KTFFKNSSK PL*PLSKRFLLLPKAFCRF*VGMQ*CGLVLAYT AHACYHRKYFKATTYKY*S*AIREKC*RGIGRF C*RW*EED*IAYW*TRSTGGRPQES*RNSRKT*C FH*SSSSGEIN*NRTHNQLCIHLKNKNINVFCP ASFSSAVPPF*TYNKVMG*K*SMYVTGALTISC LSNGRTVVMD*HPILLY*SDKSE*YNWYGH*VQS LKIRNLFSVCCLICGGTRLMD*LRLLNVQFLFQ KYNARCFEIKLI*QLFKVINCI*NHSSLLNHCM CL*YSIPRNYLTMIIQFIFTT*KKNG*QKNP*NR LIWIVTFSERNFNPS*PAKKFALEAKSVPAYLF DSVAVFSLSISI*N*FILDVVYLR*AFC**WFF SC*QSHRIMTQDEED*G*SID*FHLEYYQTFQLG IRKRLSFIRLF*IEIISTIKVMLTIPLSAE*RK I*FNLLQFNYNTTFTTFSCVLNKYFLIG*RTFK QRNAFFT*NVYLICCLFTKPLLC**KCPLCDVFD FTVC*ILFSWSCFLVTAPLLLPILLFYINACFV VIPQDVTLGMLG*GYEKILPKPGIQCMFVLYCL IRKVGPALSSTDALVDA*SGTFFISFRSRDRSE* KGRRALLCSIHRLGDDLTGYTPLTSAYRLMHVN EYFCSCKA*QLQLHIYF*RIKQDHIYLLLSTIE RKTFSCISTDFSQGKG*Y*SLLLSHPFV*VLKRD GGRSSFL*GDQYCNVCE*MITINITKSPT*ES* RPLHAPQ*CGFFHGFFFLLFS*SHPKHALFFLE S*VFINCRCY*KKIKFSLKSKPCVLCL*FKSQK* F*FKQKDTKYTEVKFELATESFVFMSEFAKSGV DKALYGRLNNCK*MISKLNLARTSILKISVPRQ CTARNKWPLQLPRLTHCAIVAGGTVNLSSSL*YC PLCIPIFYSFFRRLLSANYSASKCK*MI*K*VG NMAESGASQPHNVGI*HRMRVRV*V*KIKSLVN NFVFIYSRCMYLRKEIWYFCFPIKGIKVMVFLSV L*AGLCYSSSSMEMCFKICLPFE*SWLYVVEIF ETYLVFTCAL*MNFVLFF*NLHITCRYYCNLYF A*A*SKVICVDE*LKNI*ITL*FYYWRASFKFFS VLTRERETCIPRVII*PHSITRFMV*QALSVAF L*MLEFHMPCISQLYSMVNRCFFSHRGTCQNIL LFSLVKYIKKVF*FHFAA

Sequence OPA1.bAug05

GACGGACTGAGTACGGGTGCCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTGCCCTTGCTGCTGAGGGCCACT TCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTCACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTG GCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTG GTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTC CTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTA TCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATT GTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATTTTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACT TTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTTTTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTT GTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATTCTCTTA CAACAACAAATTCAAGAGCATGAAGAGGAAGCGCGCAGAGCCGCTGGCCAATATAGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACAAAGAGA AAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGT ATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCC AGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATAT TCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGA GTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAG ACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACA CAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTAT TGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGC AGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAG CTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGC AGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAAT AACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAA AACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGG AACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAA TTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGT GGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTT TATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAAT CGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAA TAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAA AACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTT TGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTAT TGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGA AAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACG TCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAAC CATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCAT TAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGT TTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAA TCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAAC CCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAG CTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATA GTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACA TTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTG AATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAG GACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGT GATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATG CATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTT ATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGAC AGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAA TGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTC AACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTT TTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACAT GAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATT TTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAA AGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAA AGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGT ACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGT TTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAA AATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATC TTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTT TTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTG TTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAA CTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCACATTATAATTCTATTATTGGAGAGCATCTTTT AAATTTTTTTCTGTTTTAACGAGGGAAAGAGAAACCTGTATACCTAGGGTCATTATTTGACCCCATAGTATAACCAGATTCATGGTCTAACAAGCTCTCA GTGTGGCTTTTCTCTGAATGCTTGAATTTCACATGCCTTGCATTTCACAGTTGTACTCCATGGTCAACCGGTGCTTTTTTTCACATCGTGGTACTTGTCA AAACATTTTGTTATTTTCCTTGGTAAAATATATAAAAAAGGTTTTCTAATTTCACTTTGCTGCC

OL001014 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

DGLSTGACQALAEVHAPLGGPRPRLCALAAEGH FLGHSWTGSRAGAHTGAPAWPSRRLRDLPAGGMW RLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHL VSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNL PLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWI VPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPD FDKIVESLSLLKDFFTSGHKLVSEVIGASDLLLL LGSPEETAFRATDRGSESDKHFRKGLLGELILL QQQIQEHEEEARRAAGQYSTSYAQQKRKVSDKE KIDQLQEELLHTQLKYQRILERLEKENKELRKLV LQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDA SYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARI FPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSRE FDLTKEEDLAALRHEIELRMRKNVKEGCTVSPE TISLNVKGPGLQRMVLVDLPGVINTVTSGMAPD TKETIFSISKAYMQNPNAIILCIQDGSVDAERSI VTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPS RIQQIIEGKLFPMKALGYFAVVTGKGNSSESIE AIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLA VSDCFWKMVRESVEQQADSFKATRFNLETEWKN NYPRLRELDRNELFEKAKNEILDEVISLSQVTP KHWEEILQQSLWERVSTHVIENIYLPAAQTMNSG TFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEE FSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHK WNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYF MEEALQARLKDTENAIENMVGPDWKKRWLYWKN RTQEQCVHNETKNELEKMLKCNEEHPAYLASDE ITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLK TALNHCNLCRRGFYYYQRHFVDSELECNDVVLF WRIQRMLAITANTLRQQLTNTEVRRLEKNVKEV LEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQE KLDAFIEALHQEK*IKIVLIISSAYI*RTKTST SFVQPLFLLLFHLSKHTIKSWDKNNRCMLRAL* PSAASRMEEQW*WINILFCCTKVTNRNNIIGMAI RFSP*R*ETCSLFVVLFVVALV*WIN*GCSMFS FFSRNTMLGVLK*NLYSNCLKLSIVYKITVAC* IIVCVCSILFPETI*P**FSLYSPHERKMGNRRT LKTG*FGL*RSVKEISTLHSQRRNLPWKPSQYQ LTYLIQLLFSHSLYPFEIDLF*MLYTYVRLSVN SGFSPVDRATGL*HRMRKIKDNQLTNFI*NIIKH FN*VSEKGFLS*DYFK*KLFQQLK*C*PSPSQL NKEKFSSIYCNLITILPSQHFHVF*INIF*LAK GHSSKEMLSLLKMSISFAAFSLSLYFVNKSVHCV MFLILQFAKSYFLGVAFW*QPHCYSPFYCFTSM HASL*SLKM*HLVCSVEDMKKYFRNQEFNVCLF YTV**EK*VQP*AAQMRW*MHSQELFLFLLGLGT GVNRKGGELYYVLYTD*EMTLLGTPL*PVLTG* CMLMNIFAVVKHNNYNYTSISKE*NRTIFIYFC QL*KERPSAVFPQISPKEKANISHYCYHIPLYKF *KEMEGDLHFFEEISIVTYVNR**QLILLKVPH ESPDALSMPHSNVASFMGFFFFFLADLILSMLY FSLKARYLSTADVIERK*NSVSRVNPVSCVCSSK VRNDSNLNKKILNIQKLNSN*PQNHLFLCQNLQ RVEWTKLCMED*TTVNR*YPNLIWLGLQF*KSV YLGSAQHEISGPCSFPV*PTVL*LRVEQSTFLVV YDIALFVFPFSTVFSADFFLQIIQPPNANE*YK NK*GTWQRVVLPSLTMWEFDIG*ESEYRFKR*N L*LIILYLFILDVCI*GKKSGIFAFQ*RGSK*WF FSQFSKLVYVIALAVWKCALKYAYLLNDHGYML LRYLKLTLFSLVHCE*TLYYFFKTFTLRVDIIA TYILPELDQRSFV*MSN*KIFKSHYNSIIGEHLL NFFLF*RGKEKPVYLGSLFDPIV*PDSWSNKLS VWLFSECLNFTCLAFHSCTPWSTGAFFHIVVLV KTFCYFPW*NI*KRFSNFTLL

Frame 1

TD*VRVPVRLLRKSMRHWEGLGRGSVPLLLRAT SWVIPGPGAGLGLTRGLPRGRLGACVTSPPAGC GDYVGPLWPVRSASL**NTALE*KEVYHYKNYIW FHEAFIIHIILP*SFNDPN*GHPFSSSLL*QTF LYVN*NSLQLNMATSLAGIFGQQD*LRDS*NFA ISY*DRLLGVATQPKRLLISGKI*YRTLVNINGL CLTLCGKLMSISILRKLEKPFLVQKTL*S*HQT LTRLLKALAY*RTFLPQVTNWLVKS*ELLTYFS C*VLRKKRRLEQQIVDLKVTSILERVCLVSSFSY NNKFKSMKRKRAEPLANIARAMPNRSARCQTKR KLTNFRKNFCTLS*SIRESWND*KRRTKN*EN* YCRKMTKAFIIESLRNL*LTCILKFLMFSLIMMP VIIRKIICHGLLWLEIRVLERLVCWK*LPKLEY SQEDLGR**HVLQLR*L*VKVLTMWPYLKIVLG SLILPKKKILQH*DMK*NFE*GKM*KKAVPLALR PYP*M*KALDYRGWCLLTYQV*LIL*HQAWLLT QRKLFSVSAKLTCRILMPSYCVFKMDLWMLNAV LLQTWSVKWTLMEGEPYSF*PK*TWQRKM*PVQA GFSR*LKESSSQ*KL*VILLL*QEKGTALKALK L*ENMKKSFFRIQSS*RQAC*RHTK*LQEI*AL QYQTAFGKWYESLLNNRLIVSKQHVLTLKLNGRI TILACGNLTGMNYLKKLKMKSLMKLSV*ARLHQ NIGRKSFNNLCGKEYQLM*LKTSTFQLRRP*IQ ELLTPQWISSLNSGLINNFLIKQ*RLLGRPYKKN FPAL*QNRKGKSMMTYLINLKRLLRKKVLNDTS GMTLRRTA*GLFNTMLWKTDPYLINSNGMQLFI LWKRLCRLVSRILKMQLKTWWVQTGKRGGYTGRI GPKNSVFTMKPRMNWRRC*NVMRSTQLILQVMK *PQSGRTLNPEE*K*IQA*LRILGIKFIEDIF* KQL*TIVTFVEEVFITTKGIL*ILSWNAMMWSCF GVYSACLLSPQIL*GNNLQILKLGD*RKMLKRY WKILLKMVRRRLNCLLVNAFNWRKTSRKLEKFK KNLMLSLKLFIRRNKLKSYS*SALHTSEEQKHQR LLSSLFFFCCSTFLNIQ*SHGIKIIDVCYGRFN HQLPLEWKNSGNGLTSYFVVLK*QIGII*LVWP LGSVLEDKKLVLCLLSYLWWHSFNGLTEVAQCSV SFPEIQC*VF*NKTYIAIV*SYQLYIKSQ*PAK SLYVSVVFYSQKLFDHDNSVYIHHMKEKWVTEE PLKQVNLDCNVQ*KKFQPFIASEEICLGSQVSTS LPI*FSCCFLTLYIHLKLIYFRCCILTLGFLLI VVFLLLTEPPDYDTG*GRLRIIN*LISFRILSN ISTRYQKKAFFHKTILNRNYFNN*SNVDHPPLS* IKKNLVQFIAI*LQYYLHNIFMCFK*IFFNWLK DIQAKKCFLYLKCLSHLLPFH*AFTLLIKVSIV *CF*FYSLLNLIFLELLFGNSPIATPHFIVLHQC MLRCDPSRCNTWYARLRI*KNTSETRNSMYVCF ILFDKKSRSSLKQHRCAGRCIVRNFFYFF*V*G QE*IEREESSIMFYTQIRR*PYWVHPSNQCLQVN AC**IFLQL*SITITTTHLFLKNKTGPYLFTSV NYRKKDLQLYFHRFLPRKRLILVTTVITSLCIS FKKRWREIFISLRRSVL*RM*IDDNN*YY*KSHM RVLTPSPCPTVMWLLSWVFFSSF*LISS*ACFI FP*KLGIYQLQMLLKENKIQSQE*TLCLVSVVQ KSEMILI*TKRY*IYRS*IRTSHRIICFYVRICK EWSGQSSVWKTEQL*IDDIQT*FG*DFNFKNQC T*AVHSTK*VALAASPFNPLCYSCGWNSQPF** FMILPSLYSHFLQFFPQTSFCKLFSLQMQMNDIK ISREHGREWCFPASQCGNLT*DESQSIGLKDKI FS**FCIYLF*MYVSEERNLVFLLSNKGDQSNG FSLSSLSWSML*L*QYGNVL*NMLTF*MIMAICC *DI*NLPCFHLCTVNELCIIFLKPSHYV*ILLQ LIFCLSLIKGHLCR*VIKKYLNHIIILLLESIF *IFFCFNEGKRNLYT*GHYLTP*YNQIHGLTSSQ CGFSLNA*ISHALHFTVVLHGQPVLFFTSWYLS KHFVIFLGKIYKKGFLISLCC

Frame 2

RTEYGCLSGSCGSPCAIGRASAAALCPCC*GPL PGSFLDREPGWGSHGGSRVAVSAPA*PPRRRDV ATTSGRCGL*GLPVFSETQLWNKRKFTTTKTTS GFTKHLSFTSSYLKASTTPIKDILSAVLFSDKPS FT*TEILSN*IWLPASQEFLASKISYETLKTSL SHTRIGCWGWLHSQKDF*SVERYDTGP**I*MD CA*HCVGN**VYRF*EN*KSPS*FRRPCKVSTRL *QDC*KP*LIEGLFYLRSQIG**SHRSF*PTSL VRFSGRNGV*SNRSWI*K*QAF*KGSAW*AHSL TTTNSRA*RGSAQSRWPI*HELCPTEAQGVRQRE N*PTSGRTSAHSVEVSENLGTIRKGEQRIEKIS IAER*QRHSS*KA*EIFD*HVF*SS*CSL*L*C QL*YARSSATGCCGWRSECWKD*CVGNDCPSSNI PKRIWGDDDTFSS*GDSE*RSSPCGPI*R*FSG V*SYQRRRSCSIKT*NRTSNEEKCERRLYR*P* DHILKCKRPWTTEDGAC*LTRCD*YCDIRHGS*H KGNYFQYQQSLHAES*CHHTVYSRWICGC*TQY CYRLGQSNGPSWKENHIRFDQSRPGREKCSQSK QDSADN*RKALPNESFRLFCCCNRKREQL*KH*S YKRI*RRVFSEFKAPKDKHAKGTPSDYKKFKPC SIRLLLENGTRVC*TTG**FQSNTF*P*N*MEE *LSSPAGT*PE*TI*KS*K*NP**SYQSEPGYTK TLGGNPSTIFVGKSINSCD*KHLPSSCADHEFR NF*HHSGYQA*TVD**TTS**SSRGCLGDPTRR IFPLYDRTERERA**HI**T*RGC*GRKY*TTQV E*LCGGQLEGYSTQCFGRPIHI**TAMGCSYLF YGRGSAGSSQGY*KCN*KHGGSRLEKEVVILEE SDPRTVCSQ*NQE*IGEDVEM**GAPSLSCK**N NHSPEEP*IPRSRSRSKLD*GYLASSL*KTFFK NSSKPL*PLSKRFLLLPKAFCRF*VGMQ*CGLV LAYTAHACYHRKYFKATTYKY*S*AIREKC*RGI GRFC*RW*EED*IAYW*TRSTGGRPQES*RNSR KT*CFH*SSSSGEIN*NRTHNQLCIHLKNKNIN VFCPASFSSAVPPF*TYNKVMG*K*SMYVTGALT ISCLSNGRTVVMD*HPILLY*SDKSE*YNWYGH *VQSLKIRNLFSVCCLICGGTRLMD*LRLLNVQ FLFQKYNARCFEIKLI*QLFKVINCI*NHSSLLN HCMCL*YSIPRNYLTMIIQFIFTT*KKNG*QKN P*NRLIWIVTFSERNFNPS*PAKKFALEAKSVP AYLFDSVAVFSLSISI*N*FILDVVYLR*AFC** WFFSC*QSHRIMTQDEED*G*SID*FHLEYYQT FQLGIRKRLSFIRLF*IEIISTIKVMLTIPLSA E*RKI*FNLLQFNYNTTFTTFSCVLNKYFLIG*R TFKQRNAFFT*NVYLICCLFTKPLLC**KCPLC DVFDFTVC*ILFSWSCFLVTAPLLLPILLFYIN ACFVVIPQDVTLGMLG*GYEKILPKPGIQCMFVL YCLIRKVGPALSSTDALVDA*SGTFFISFRSRD RSE*KGRRALLCSIHRLGDDLTGYTPLTSAYRL MHVNEYFCSCKA*QLQLHIYF*RIKQDHIYLLLS TIERKTFSCISTDFSQGKG*Y*SLLLSHPFV*V LKRDGGRSSFL*GDQYCNVCE*MITINITKSPT *ES*RPLHAPQ*CGFFHGFFFLLFS*SHPKHALF FLES*VFINCRCY*KKIKFSLKSKPCVLCL*FK SQK*F*FKQKDTKYTEVKFELATESFVFMSEFA KSGVDKALYGRLNNCK*MISKLNLARTSILKISV PRQCTARNKWPLQLPRLTHCAIVAGGTVNLSSS L*YCPLCIPIFYSFFRRLLSANYSASKCK*MI* K*VGNMAESGASQPHNVGI*HRMRVRV*V*KIKS LVNNFVFIYSRCMYLRKEIWYFCFPIKGIKVMV FLSVL*AGLCYSSSSMEMCFKICLPFE*SWLYV VEIFETYLVFTCAL*MNFVLFF*NLHITCRYYCN LYFA*A*SKVICVDE*LKNI*ITL*FYYWRASF KFFSVLTRERETCIPRVII*PHSITRFMV*QAL SVAFL*MLEFHMPCISQLYSMVNRCFFSHRGTCQ NILLFSLVKYIKKVF*FHFAA