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Sequences

Sequence BMF.bAug05

GGAGTCGCGCTGCGCACGGGGGCCCGGGAAGGGTTTGGGCTGCGGACGTCTGGGGTGACCATGAAGAACTGGGGCGCTGGGGTGAGGGAACGGGGTGCAG AACCCACAGTTGGGTGCGCTGCTCCAAGAGGGAACTTGGGGGTAGCATTTGGGACTCGAAGGAGTTGGGTCCAGGTAGGCAGGGGAACCTGGGGTGCACT GCTGAGCAGACCTGGGCGCCCGAGAACGGCCCAGCCTCTGGGTCTGACTTTGGGATAGGACAGGAGCTGTGGGGCATCCTGCCTCTCTGCGGAGTCTGAA GGCTCCTCTCCTTAACAGCTGGGCTTTTTCCCTCCTTCCCAATCGAGTCTGGGCGTCCAGCCCCCGAGTGCTCGTCACGCTGGACCCTGGCGCGGAGCCC TGGCATCACGACTCGGAGGCCGAGACTCTCTCCTGGAGTCACCCAGGAGAGATGGAGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAAC CAGAGGATGGGGAGCCGGTGACCCAACCCGGGAGCTTGCTCTCTGCTGACCTGTTTGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTT CCCTCTCACCCACTGCTGTGGCCCTGGCCTTCGACCCACCAGCCAGGAAGACAAAGCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATG CTGCCTTGTGGGGTGACTGAGGAACCCCAGCGACTCTTTTATGGCAATGCTGGCTATCGGCTTCCTCTCCCTGCCAGTTTCCCAGCAGTCTTGCCCATTG GGGAGCAGCCCCCCGAAGGGCAGTGGCAACATCAAGCAGAGCACCAGCAGAACCAAAATCGTGTGTGGTGGCAGATCCTCCTCTTCCTGCACAACCTTGC TTTGAATGGAGAAGAGAACAGGAACGGGGCAGGCCCTAGGTGAGGGTGGGCTGCCCTCTTCACATGGGGCACCAGGAACACCGTCTGGAACAGGAAGGAC ATCGGGCAGGACTGACACTGTGTCTTGTGAAATTGTTTTTTTGTTGTTATTTTGTGTTTTAATTTTTTTTAATTTCTCTCTGAGTGTACATACAACATAC TCAAGCGGGACCTTCTTTCTCTGTCAGGCCCTTGACCTGGAATGGGGGCCTTTGTCAAACACTGTTGAAGGAGAGGCTGATGTGTCTGTGATGGTGAGAA TTCCCAAGGGCTCTGACAAGTAGATTCTTCGACTGAGGAATCTACCAGTTGTCGAAGATGATCCGTTAGTGATGTTCTCTGGGAAGTGGACTGTGGTTTT TCCAGAGGAACTCAGTTAAGAAATCGAGAGTGGATTAGACTCCCAGTTCCACCAAACCTATGAGCCTTCCACTGTGGATGGGGGCCGTGATCCTGATGGT CACATTGCTTTAACCCAGCAGGGCTTCGGCCAGGGGCTTTCCACTTGAGGATAGCAGCTTCACTAGGCTGGCCGGCCAGCTCCACATCTGACTGGGTTCT TACTTCTCAGCCAGTACCTGCCCCATGGGCTCAAGGATTCCTGGCCAGCTCCTGCCACCTCCAGCAGACCTCAGGGAGGGTTGAGTTTCTCTAAGGACCC CTCAAACATGTCGCAAAATGAACCAAACTTCTGGCTAGGCCTCAAAACTGACTTGGTCCCACTTGGAGGCCCCAGGATTGGTCCTGAGGTACAGAGCCAC TGCCACCACTGGCGGCCTGGGACCAGCTGGGTGTCAGCCACGGATGAGCCGAATAGCCAGTCAGCATGTTGCTGCTGGCAGCCTGTGCCTTTGTCAGCTC TTCTTTCAAAAGACCCCACCGACAGACCGCATTCCACCCCCAACATCGGCACTGAGGGACATCGGGGGCAAGTTTGCAGTGGGGCCGGAAAATATGGTGG CCACCCTACCATGAGAGTCAGCCGTAGGGGACCCCAGACCCCTTGGTTTCCTTGGAAACAACATACCTCTTCCCCCTTATCCCCAGTCCTTTTTCATACC TAGTGGGATACAGAAGAAGCCAGGACAGTGGCTTTGCCAGCTAAGTGACTTTTAGAGTAACAGATAACGATTAGAGTGGGGAACCGTCAAAGCTGGGTAC ACATTTCCTATCTTCCTCCAGCTTCCAGCTAGGCCAGAAGGGCATGCTCTGGAACCCAGCAGGATCACAGCTGCCTGTGCACGTCTTCCCTTCTCTCCCT GCTGCGGCACTTATGGAGAGGATCTAAAGCAGCCGGTGTGGCAGCTCCGATAGCAGGCACAGGGAATCTGTTAACCAGACGCTAGAAACTAAATTATAAC TTTTGCATATGTGAGAAAAGACAACATTGGTGCTAACAGTGAAGTAAGGCCCAAAGGAAAGACGGCTTCCCTGGACAAAGAAGACCTCAGGGCTACCCAA GGAAATAGGAGGAGTCTAGAGTAGACTCACAAATCTGAACAAGCCCAAGTCTTCCAGTTCTGAGGAGAGGAGGTCTTCAGTACTATTGAAGGAGACATTG ATCTTCTGGATGTACAGTTGTGCAGGTTGTTCACTGCACAAGGGCACCTGCAGCTAATGGAGGCTGGAATTCAGCTTGTGTTCTGCTCACTAAGCTGTGT GCGTGCCCTGGTGTGGGGCTACTTCTCCCAAGAAGAAGGGGTGCCTTTCCTAATTTGCAAAGGTGCCATATGGGCTCACAGCACCCTGGAGGAGAAGGGC CTCAAACTGTGCATGTAAGCCTTTGTTTTGTTCTGCTCAGATATTCTGGATATTACCTCTTCTTGGTCAGAATTCTATTCTCAGGGTGTGTACCATGATT TGTCTGCTAAGAAGACGGGTTCCTGCTTTGCAGAGAAGGAGCCGGGGACCAGCTTGAAGACTGGCTCTGGGACACACTGACCATTGTGAAATTCAGCCTT CCCTGTGGGTCTCCATACCATTTTATGATGTGTATGGGACATACGTTGTTCTCCCCTCTCTGGATCAAGGTGGTGACAGGCAGCCTGCTGCCGTATGCTT CAGTGGCACACACCAAAAAACCGGGATTATTTACAGCAGCCACTATTCACCCCTTTTGGCAGACTCTCCACCTGAGTTCAGTGAGAGAGAAAATGATTTA GATCTTGGTGCTGGGGCAAGGCCATCAGCTTCAGAGACCTTGCCAGGCCCAGGCTGGGTGCCCTGTGGCCTGAGAACTGAGCCCAGACTTTGACAAACCC ACCTCAACATCAAGCCTGGGGCAGGTGGAAGGTGGTCTGACTGCACTGTCTCCATCTTATGCAAAGCCAGGAACTCACTCACTGTCTTAAGTGAGCCAGG GCAAAATTTACACCCCTCACGTTTCCCTCTCCCTTCTTCCCCACCAACAAAACCCAGGAGGTAGCTGGCAGAGTAGCTGTCAGGAAGGAGAAAGGTTCTT TCTGGCTGGTTGTTTTTAATTGGCTTAGTGATCTAAACTGGCCCCTTCCTCCTCTGCCTGGTGAGTTGGCCTAAACATTCCTCAAGTCTAGCCTCAGGAG ACCTGCCCCTCCCCCCCGACCTCCACCCCCTCAGACTCCATCCCCCACCCCCAGGAGCCTGGCTGTCTCTAGCGAGGGGTGGAGAATCAGATTTAGAGGG AGGGAGAGTCTGACCTGGATCCTGACCTGTAACCAGCTGAAGACGGTGGAGGCTTTGTGGCTTGCTGAGGGTGGGGGTTGGGAGAGGGACTGGAAACCTT CCTCCTCGGGAAAGAAATGCCTGGGAGGAAGGGAAGCCTGATATTCAGGGTCAAAACAGCCCTTCTAATTCACAACCCCAAAGCAGGGGTTTCTAGAAGT TGGTCAACAGATCTGGTGAGGGAAGTCCCCAGGCCAGACGCTGGACTCCTGCAATGAGGGTGGAGGACTCAGCCTGGCCTCTGCCTGGCCTGTTTCAAAG TCTGGGCCACTGGCAGCCTTCTCTTGCTCAATCCGAGGTGGAAAAAGATCACCTCCCAGTGACCTCCCTCAGCCCCCAGGGGAGTAGGTCTGTGTCCAAA GCTATGTGGCAGGCTTGTTTGAAGGACCCAGAGGGGCCCAGCTGACCTGTCTCACTGCTCCTGGCAGCCCAGCCCCATCGGCAGGTGGCCCCTGCCTGGG GGTTATTCGGGATGGTTCAGCCAGGGCCTCCCAGGAAGAGTGCTGTGGAGCCACGGTGACTGTCCTGGACAGCAAGGAGCATGCTACCCCAGTGAGCACT TCTTTTTGAGGGACTTGATGGGGAGGTGGGGTAGGCAGAAGGGACGTGGCAGAAGCGGGAAGACTTTGGTCACCATGGCTCTGCAGGCCTTCTGCAAATC AGTGCTGGCCTGGGCTGGAAACAGCTCTGTGTGTGAAGGTGAGGACTCTTGGAAGCAGGCCATCCTGGCCAGACACCCTAGCAGGAAGGGGCTCACCTGT CACCCTTAGGCAGCCTGAGGGCTGGTGGGGACTTTTGAGTCTTGAGGGGATAGCGGAAAAAGCTGAGCTCATAGGTGCCCAGCCAGCCTCCCAGCTAAAG GTGCTCAGAGCCCCACTGCCCCCTCTCTCTGTGCAGGTGGCCAGTGCCCCTCCCTGCTCTGGGAGCATTGCTAGCCTTCTACCCCATCCCTGGATCCACA GGGGCTATCGAGGAGACCCAGTGAGAATGTAGCATTTTGTTCATCCCCAGGGTAGCTGCCCTGGGGTCTGGGCACTCTGCCTCTGGGAGAGAGGAAGAAG AAAGGGGCCCCATTTTTTAAAAAACTGTACAGAGCCTTTGGCTTTATGTGTTTATGTTCTTCACATGCATATGTGTGTATGTGTGTATATCTTTCCCCCC ATCAATTGGTACAATTTTTAATAAAATCATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Alternative donor

Frame 0

GVALRTGAREGFGLRTSGVTMKNWGAGVRERGA EPTVGCAAPRGNLGVAFGTRRSWVQVGRGTWGAL LSRPGRPRTAQPLGLTLG*DRSCGASCLSAESE GSSP*QLGFFPPSQSSLGVQPPSARHAGPWRGA LASRLGGRDSLLESPRRDGAISVCGGAGG*CVPT RGWGAGDPTRELALC*PVCPEPTGLPPQPTSAL PSHPLLWPWPSTHQPGRQSYPDSQPSLPQPRCH AALWGD*GTPATLLWQCWLSASSPCQFPSSLAHW GAAPRRAVATSSRAPAEPKSCVVADPPLPAQPC FEWRREQERGRP*VRVGCPLHMGHQEHRLEQEG HRAGLTLCLVKLFFCCYFVF*FFLISL*VYIQHT QAGPSFSVRPLTWNGGLCQTLLKERLMCL*W*E FPRALTSRFFD*GIYQLSKMIR**CSLGSGLWF FQRNSVKKSRVD*TPSSTKPMSLPLWMGAVILMV TLL*PSRASARGFPLEDSSFTRLAGQLHI*LGS YFSASTCPMGSRIPGQLLPPPADLREG*VSLRT PQTCRKMNQTSG*ASKLTWSHLEAPGLVLRYRAT ATTGGLGPAGCQPRMSRIASQHVAAGSLCLCQL FFQKTPPTDRIPPPTSALRDIGGKFAVGPENMV ATLP*ESAVGDPRPLGFLGNNIPLPPYPQSFFIP SGIQKKPGQWLCQLSDF*SNR*RLEWGTVKAGY TFPIFLQLPARPEGHALEPSRITAACARLPFSP CCGTYGEDLKQPVWQLR*QAQGIC*PDARN*IIT FAYVRKDNIGANSEVRPKGKTASLDKEDLRATQ GNRRSLE*THKSEQAQVFQF*GEEVFSTIEGDI DLLDVQLCRLFTAQGHLQLMEAGIQLVFCSLSCV RALVWGYFSQEEGVPFLICKGAIWAHSTLEEKG LKLCM*AFVLFCSDILDITSSWSEFYSQGVYHD LSAKKTGSCFAEKEPGTSLKTGSGTH*PL*NSAF PVGLHTIL*CVWDIRCSPLSGSRW*QAACCRML QWHTPKNRDYLQQPLFTPFGRLST*VQ*ERK*F RSWCWGKAISFRDLARPRLGALWPEN*AQTLTNP PQHQAWGRWKVV*LHCLHLMQSQELTHCLK*AR AKFTPLTFPSPFFPTNKTQEVAGRVAVRKEKGS FWLVVFNWLSDLNWPLPPLPGELA*TFLKSSLRR PAPPPRPPPPQTPSPTPRSLAVSSEGWRIRFRG RESLTWILTCNQLKTVEALWLAEGGGWERDWKP SSSGKKCLGGREA*YSGSKQPF*FTTPKQGFLEV GQQIW*GKSPGQTLDSCNEGGGLSLASAWPVSK SGPLAAFSCSIRGGKRSPPSDLPQPPGE*VCVQ SYVAGLFEGPRGAQLTCLTAPGSPAPSAGGPCLG VIRDGSARASQEECCGATVTVLDSKEHATPVST SF*GT*WGGGVGRRDVAEAGRLWSPWLCRPSAN QCWPGLETALCVKVRTLGSRPSWPDTLAGRGSPV TLRQPEGWWGLLSLEGIAEKAELIGAQPASQLK VLRAPLPPLSVQVASAPPCSGSIASLLPHPWIH RGYRGDPVRM*HFVHPQGSCPGVWALCLWERGRR KGPHFLKNCTEPLALCVYVLHMHMCVCVYIFPP INWYNF**NHLKQ

Frame 1

ESRCARGPGKGLGCGRLG*P*RTGALG*GNGVQ NPQLGALLQEGTWG*HLGLEGVGSR*AGEPGVH C*ADLGARERPSLWV*LWDRTGAVGHPASLRSLK APLLNSWAFSLLPNRVWASSPRVLVTLDPGAEP WHHDSEAETLSWSHPGEMEPSQCVEELEDDVFQ PEDGEPVTQPGSLLSADLFAQSLLDCPLSRLQLF PLTHCCGPGLRPTSQEDKATQTLSPASPSQGVM LPCGVTEEPQRLFYGNAGYRLPLPASFPAVLPI GEQPPEGQWQHQAEHQQNQNRVWWQILLFLHNLA LNGEENRNGAGPR*GWAALFTWGTRNTVWNRKD IGQD*HCVL*NCFFVVILCFNFF*FLSECTYNI LKRDLLSLSGP*PGMGAFVKHC*RRG*CVCDGEN SQGL*QVDSSTEESTSCRR*SVSDVLWEVDCGF SRGTQLRNREWIRLPVPPNL*AFHCGWGP*S*W SHCFNPAGLRPGAFHLRIAASLGWPASSTSDWVL TSQPVPAPWAQGFLASSCHLQQTSGRVEFL*GP LKHVAK*TKLLARPQN*LGPTWRPQDWS*GTEP LPPLAAWDQLGVSHG*AE*PVSMLLLAACAFVSS SFKRPHRQTAFHPQHRH*GTSGASLQWGRKIWW PPYHESQP*GTPDPLVSLETTYLFPLIPSPFSY LVGYRRSQDSGFAS*VTFRVTDND*SGEPSKLGT HFLSSSSFQLGQKGMLWNPAGSQLPVHVFPSLP AAALMERI*SSRCGSSDSRHRESVNQTLETKL* LLHM*EKTTLVLTVK*GPKERRLPWTKKTSGLPK EIGGV*SRLTNLNKPKSSSSEERRSSVLLKETL IFWMYSCAGCSLHKGTCS*WRLEFSLCSAH*AV CVPWCGATSPKKKGCLS*FAKVPYGLTAPWRRRA SNCACKPLFCSAQIFWILPLLGQNSILRVCTMI CLLRRRVPALQRRSRGPA*RLALGHTDHCEIQP SLWVSIPFYDVYGTYVVLPSLDQGGDRQPAAVCF SGTHQKTGIIYSSHYSPLLADSPPEFSERENDL DLGAGARPSASETLPGPGWVPCGLRTEPRL*QT HLNIKPGAGGRWSDCTVSILCKARNSLTVLSEPG QNLHPSRFPLPSSPPTKPRR*LAE*LSGRRKVL SGWLFLIGLVI*TGPFLLCLVSWPKHSSSLASG DLPLPPDLHPLRLHPPPPGAWLSLARGGESDLEG GRV*PGS*PVTS*RRWRLCGLLRVGVGRGTGNL PPRERNAWEEGKPDIQGQNSPSNSQPQSRGF*K LVNRSGEGSPQARRWTPAMRVEDSAWPLPGLFQS LGHWQPSLAQSEVEKDHLPVTSLSPQGSRSVSK AMWQACLKDPEGPS*PVSLLLAAQPHRQVAPAW GLFGMVQPGPPRKSAVEPR*LSWTARSMLPQ*AL LFEGLDGEVG*AEGTWQKREDFGHHGSAGLLQI SAGLGWKQLCV*R*GLLEAGHPGQTP*QEGAHL SPLGSLRAGGDF*VLRG*RKKLSS*VPSQPPS*R CSEPHCPLSLCRWPVPLPALGALLAFYPIPGST GAIEETQ*ECSILFIPRVAALGSGHSASGREEE ERGPIF*KTVQSLWLYVFMFFTCICVYVCISFPP SIGTIFNKII*SK

Frame 2

SRAAHGGPGRVWAADVWGDHEELGRWGEGTGCR THSWVRCSKRELGGSIWDSKELGPGRQGNLGCT AEQTWAPENGPASGSDFGIGQELWGILPLCGV* RLLSLTAGLFPSFPIESGRPAPECSSRWTLARSP GITTRRPRLSPGVTQERWSHLSVWRSWRMMCSN QRMGSR*PNPGACSLLTCLPRAYWTAPSADFSS SLSPTAVALAFDPPARKTKLPRLSAQPPPAKVSC CLVG*LRNPSDSFMAMLAIGFLSLPVSQQSCPL GSSPPKGSGNIKQSTSRTKIVCGGRSSSSCTTL L*MEKRTGTGQALGEGGLPSSHGAPGTPSGTGRT SGRTDTVSCEIVFLLLFCVLIFFNFSLSVHTTY SSGTFFLCQALDLEWGPLSNTVEGEADVSVMVR IPKGSDK*ILRLRNLPVVEDDPLVMFSGKWTVVF PEELS*EIESGLDSQFHQTYEPSTVDGGRDPDG HIALTQQGFGQGLST*G*QLH*AGRPAPHLTGF LLLSQYLPHGLKDSWPAPATSSRPQGGLSFSKDP SNMSQNEPNFWLGLKTDLVPLGGPRIGPEVQSH CHHWRPGTSWVSATDEPNSQSACCCWQPVPLSA LLSKDPTDRPHSTPNIGTEGHRGQVCSGAGKYGG HPTMRVSRRGPQTPWFPWKQHTSSPLSPVLFHT *WDTEEARTVALPAK*LLE*QITIRVGNRQSWV HISYLPPASS*ARRACSGTQQDHSCLCTSSLLSL LRHLWRGSKAAGVAAPIAGTGNLLTRR*KLNYN FCICEKRQHWC*Q*SKAQRKDGFPGQRRPQGYP RK*EESRVDSQI*TSPSLPVLRRGGLQYY*RRH* SSGCTVVQVVHCTRAPAANGGWNSACVLLTKLC ACPGVGLLLPRRRGAFPNLQRCHMGSQHPGGEG PQTVHVSLCFVLLRYSGYYLFLVRILFSGCVP*F VC*EDGFLLCREGAGDQLEDWLWDTLTIVKFSL PCGSPYHFMMCMGHTLFSPLWIKVVTGSLLPYA SVAHTKKPGLFTAATIHPFWQTLHLSSVREKMI* ILVLGQGHQLQRPCQAQAGCPVA*ELSPDFDKP TSTSSLGQVEGGLTALSPSYAKPGTHSLS*VSQ GKIYTPHVSLSLLPHQQNPGGSWQSSCQEGERFF LAGCF*LA**SKLAPSSSAW*VGLNIPQV*PQE TCPSPPTSTPSDSIPHPQEPGCL*RGVENQI*R EGESDLDPDL*PAEDGGGFVAC*GWGLGEGLETF LLGKEMPGRKGSLIFRVKTALLIHNPKAGVSRS WSTDLVREVPRPDAGLLQ*GWRTQPGLCLACFK VWATGSLLLLNPRWKKITSQ*PPSAPRGVGLCPK LCGRLV*RTQRGPADLSHCSWQPSPIGRWPLPG GYSGWFSQGLPGRVLWSHGDCPGQQGACYPSEH FFLRDLMGRWGRQKGRGRSGKTLVTMALQAFCKS VLAWAGNSSVCEGEDSWKQAILARHPSRKGLTC HP*AA*GLVGTFES*GDSGKS*AHRCPASLPAK GAQSPTAPSLCAGGQCPSLLWEHC*PSTPSLDPQ GLSRRPSENVAFCSSPG*LPWGLGTLPLGERKK KGAPFFKKLYRAFGFMCLCSSHAYVCMCVYLSP HQLVQFLIKSFKA

Sequence BMF.dAug05

ATTTTCCCAGGTACCCTGCATGCATATAGCTGCTGATTCTACTCCTGCTATTGCTCACAACCCTCAGAGTCAAACTTTGTGACCGGCCTAGGAGAGATGG AGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAACCAGAGGATGGGGAGCCGGTGACCCAACCCGGGAGCTTGCTCTCTGCTGACCTGTT TGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTTCCCTCTCACCCACTGCTGTGGCCCTGGCCTTCGACCCACCAGCCAGGAAGACAAA GCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATGCTGCCTTGTGGGGTGACTGAGGAACCCCAGCGACTCTTTTATGGCAATGCTGGCT ATCGGCTTCCTCTCCCTGCCAGTTTCCCAGCAGTCTTGCCCATTGGGGAGCAGCCCCCCGAAGGGCAGTGGCAACATCAAGCAGAGGTACAGATTGCCCG AAAGCTTCAGTGCATTGCAGACCAGTTCCACCGGCTTCATGTGCAGCAACACCAGCAGAACCAAAATCGTGTGTGGTGGCAGATCCTCCTCTTCCTGCAC AACCTTGCTTTGAATGGAGAAGAGAACAGGAACGGGGCAGGCCCTAGGTGAGGGTGGGCTGCCCTCTTCACATGGGGCACCAGGAACACCGTCTGGAACA GGAAGGACATCGGGCAGGACTGACACTGTGTCTTGTGAAATTGTTTTTTTGTTGTTATTTTGTGTTTTAATTTTTTTTAATTTCTCTCTGAGTGTACATA CAACATACTCAAGCGGGACCTTCTTTCTCTGTCAGGCCCTTGACCTGGAATGGGGGCCTTTGTCAAACACTGTTGAAGGAGAGGCTGATGTGTCTGTGAT GGTGAGAATTCCCAAGGGCTCTGACAAGTAGATTCTTCGACTGAGGAATCTACCAGTTGTCGAAGATGATCCGTTAGTGATGTTCTCTGGGAAGTGGACT GTGGTTTTTCCAGAGGAACTCAGTTAAGAAATCGAGAGTGGATTAGACTCCCAGTTCCACCAAACCTATGAGCCTTCCACTGTGGATGGGGGCCGTGATC CTGATGGTCACATTGCTTTAACCCAGCAGGGCTTCGGCCAGGGGCTTTCCACTTGAGGATAGCAGCTTCACTAGGCTGGCCGGCCAGCTCCACATCTGAC TGGGTTCTTACTTCTCAGCCAGTACCTGCCCCATGGGCTCAAGGATTCCTGGCCAGCTCCTGCCACCTCCAGCAGACCTCAGGGAGGGTTGAGTTTCTCT AAGGACCCCTCAAACATGTCGCAAAATGAACCAAACTTCTGGCTAGGCCTCAAAACTGACTTGGTCCCACTTGGAGGCCCCAGGATTGGTCCTGAGGTAC AGAGCCACTGCCACCACTGGCGGCCTGGGACCAGCTGGGTGTCAGCCACGGATGAGCCGAATAGCCAGTCAGCATGTTGCTGCTGGCAGCCTGTGCCTTT GTCAGCTCTTCTTTCAAAAGACCCCACCGACAGACCGCATTCCACCCCCAACATCGGCACTGAGGGACATCGGGGGCAAGTTTGCAGTGGGGCCGGAAAA TATGGTGGCCACCCTACCATGAGAGTCAGCCGTAGGGGACCCCAGACCCCTTGGTTTCCTTGGAAACAACATACCTCTTCCCCCTTATCCCCAGTCCTTT TTCATACCTAGTGGGATACAGAAGAAGCCAGGACAGTGGCTTTGCCAGCTAAGTGACTTTTAGAGTAACAGATAACGATTAGAGTGGGGAACCGTCAAAG CTGGGTACACATTTCCTATCTTCCTCCAGCTTCCAGCTAGGCCAGAAGGGCATGCTCTGGAACCCAGCAGGATCACAGCTGCCTGTGCACGTCTTCCCTT CTCTCCCTGCTGCGGCACTTATGGAGAGGATCTAAAGCAGCCGGTGTGGCAGCTCCGATAGCAGGCACAGGGAATCTGTTAACCAGACGCTAGAAACTAA ATTATAACTTTTGCATATGTGAGAAAAGACAACATTGGTGCTAACAGTGAAGTAAGGCCCAAAGGAAAGACGGCTTCCCTGGACAAAGAAGACCTCAGGG CTACCCAAGGAAATAGGAGGAGTCTAGAGTAGACTCACAAATCTGAACAAGCCCAAGTCTTCCAGTTCTGAGGAGAGGAGGTCTTCAGTACTATTGAAGG AGACATTGATCTTCTGGATGTACAGTTGTGCAGGTTGTTCACTGCACAAGGGCACCTGCAGCTAATGGAGGCTGGAATTCAGCTTGTGTTCTGCTCACTA AGCTGTGTGCGTGCCCTGGTGTGGGGCTACTTCTCCCAAGAAGAAGGGGTGCCTTTCCTAATTTGCAAAGGTGCCATATGGGCTCACAGCACCCTGGAGG AGAAGGGCCTCAAACTGTGCATGTAAGCCTTTGTTTTGTTCTGCTCAGATATTCTGGATATTACCTCTTCTTGGTCAGAATTCTATTCTCAGGGTGTGTA CCATGATTTGTCTGCTAAGAAGACGGGTTCCTGCTTTGCAGAGAAGGAGCCGGGGACCAGCTTGAAGACTGGCTCTGGGACACACTGACCATTGTGAAAT TCAGCCTTCCCTGTGGGTCTCCATACCATTTTATGATGTGTATGGGACATACGTTGTTCTCCCCTCTCTGGATCAAGGTGGTGACAGGCAGCCTGCTGCC GTATGCTTCAGTGGCACACACCAAAAAACCGGGATTATTTACAGCAGCCACTATTCACCCCTTTTGGCAGACTCTCCACCTGAGTTCAGTGAGAGAGAAA ATGATTTAGATCTTGGTGCTGGGGCAAGGCCATCAGCTTCAGAGACCTTGCCAGGCCCAGGCTGGGTGCCCTGTGGCCTGAGAACTGAGCCCAGACTTTG ACAAACCCACCTCAACATCAAGCCTGGGGCAGGTGGAAGGTGGTCTGACTGCACTGTCTCCATCTTATGCAAAGCCAGGAACTCACTCACTGTCTTAAGT GAGCCAGGGCAAAATTTACACCCCTCACGTTTCCCTCTCCCTTCTTCCCCACCAACAAAACCCAGGAGGTAGCTGGCAGAGTAGCTGTCAGGAAGGAGAA AGGTTCTTTCTGGCTGGTTGTTTTTAATTGGCTTAGTGATCTAAACTGGCCCCTTCCTCCTCTGCCTGGTGAGTTGGCCTAAACATTCCTCAAGTCTAGC CTCAGGAGACCTGCCCCTCCCCCCCGACCTCCACCCCCTCAGACTCCATCCCCCACCCCCAGGAGCCTGGCTGTCTCTAGCGAGGGGTGGAGAATCAGAT TTAGAGGGAGGGAGAGTCTGACCTGGATCCTGACCTGTAACCAGCTGAAGACGGTGGAGGCTTTGTGGCTTGCTGAGGGTGGGGGTTGGGAGAGGGACTG GAAACCTTCCTCCTCGGGAAAGAAATGCCTGGGAGGAAGGGAAGCCTGATATTCAGGGTCAAAACAGCCCTTCTAATTCACAACCCCAAAGCAGGGGTTT CTAGAAGTTGGTCAACAGATCTGGTGAGGGAAGTCCCCAGGCCAGACGCTGGACTCCTGCAATGAGGGTGGAGGACTCAGCCTGGCCTCTGCCTGGCCTG TTTCAAAGTCTGGGCCACTGGCAGCCTTCTCTTGCTCAATCCGAGGTGGAAAAAGATCACCTCCCAGTGACCTCCCTCAGCCCCCAGGGGAGTAGGTCTG TGTCCAAAGCTATGTGGCAGGCTTGTTTGAAGGACCCAGAGGGGCCCAGCTGACCTGTCTCACTGCTCCTGGCAGCCCAGCCCCATCGGCAGGTGGCCCC TGCCTGGGGGTTATTCGGGATGGTTCAGCCAGGGCCTCCCAGGAAGAGTGCTGTGGAGCCACGGTGACTGTCCTGGACAGCAAGGAGCATGCTACCCCAG TGAGCACTTCTTTTTGAGGGACTTGATGGGGAGGTGGGGTAGGCAGAAGGGACGTGGCAGAAGCGGGAAGACTTTGGTCACCATGGCTCTGCAGGCCTTC TGCAAATCAGTGCTGGCCTGGGCTGGAAACAGCTCTGTGTGTGAAGGTGAGGACTCTTGGAAGCAGGCCATCCTGGCCAGACACCCTAGCAGGAAGGGGC TCACCTGTCACCCTTAGGCAGCCTGAGGGCTGGTGGGGACTTTTGAGTCTTGAGGGGATAGCGGAAAAAGCTGAGCTCATAGGTGCCCAGCCAGCCTCCC AGCTAAAGGTGCTCAGAGCCCCACTGCCCCCTCTCTCTGTGCAGGTGGCCAGTGCCCCTCCCTGCTCTGGGAGCATTGCTAGCCTTCTACCCCATCCCTG GATCCACAGGGGCTATCGAGGAGACCCAGTGAGAATGTAGCATTTTGTTCATCCCCAGGGTAGCTGCCCTGGGGTCTGGGCACTCTGCCTCTGGGAGAGA GGAAGAAGAAAGGGGCCCCATTTTTTAAAAAACTGTACAGAGCCTTTGGCTTTATGTGTTTATGTTCTTCACATGCATATGTGTGTATGTGTGTATATCT TTCCCCCCATCAATTGGTACAATTTTTAATAAAATCATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Alternative donor

Frame 0

IFPGTLHAYSC*FYSCYCSQPSESNFVTGLGEM EPSQCVEELEDDVFQPEDGEPVTQPGSLLSADLF AQSLLDCPLSRLQLFPLTHCCGPGLRPTSQEDK ATQTLSPASPSQGVMLPCGVTEEPQRLFYGNAG YRLPLPASFPAVLPIGEQPPEGQWQHQAEVQIAR KLQCIADQFHRLHVQQHQQNQNRVWWQILLFLH NLALNGEENRNGAGPR*GWAALFTWGTRNTVWN RKDIGQD*HCVL*NCFFVVILCFNFF*FLSECTY NILKRDLLSLSGP*PGMGAFVKHC*RRG*CVCD GENSQGL*QVDSSTEESTSCRR*SVSDVLWEVD CGFSRGTQLRNREWIRLPVPPNL*AFHCGWGP*S *WSHCFNPAGLRPGAFHLRIAASLGWPASSTSD WVLTSQPVPAPWAQGFLASSCHLQQTSGRVEFL *GPLKHVAK*TKLLARPQN*LGPTWRPQDWS*GT EPLPPLAAWDQLGVSHG*AE*PVSMLLLAACAF VSSSFKRPHRQTAFHPQHRH*GTSGASLQWGRK IWWPPYHESQP*GTPDPLVSLETTYLFPLIPSPF SYLVGYRRSQDSGFAS*VTFRVTDND*SGEPSK LGTHFLSSSSFQLGQKGMLWNPAGSQLPVHVFP SLPAAALMERI*SSRCGSSDSRHRESVNQTLETK L*LLHM*EKTTLVLTVK*GPKERRLPWTKKTSG LPKEIGGV*SRLTNLNKPKSSSSEERRSSVLLK ETLIFWMYSCAGCSLHKGTCS*WRLEFSLCSAH* AVCVPWCGATSPKKKGCLS*FAKVPYGLTAPWR RRASNCACKPLFCSAQIFWILPLLGQNSILRVC TMICLLRRRVPALQRRSRGPA*RLALGHTDHCEI QPSLWVSIPFYDVYGTYVVLPSLDQGGDRQPAA VCFSGTHQKTGIIYSSHYSPLLADSPPEFSERE NDLDLGAGARPSASETLPGPGWVPCGLRTEPRL* QTHLNIKPGAGGRWSDCTVSILCKARNSLTVLS EPGQNLHPSRFPLPSSPPTKPRR*LAE*LSGRR KVLSGWLFLIGLVI*TGPFLLCLVSWPKHSSSLA SGDLPLPPDLHPLRLHPPPPGAWLSLARGGESD LEGGRV*PGS*PVTS*RRWRLCGLLRVGVGRGT GNLPPRERNAWEEGKPDIQGQNSPSNSQPQSRGF *KLVNRSGEGSPQARRWTPAMRVEDSAWPLPGL FQSLGHWQPSLAQSEVEKDHLPVTSLSPQGSRS VSKAMWQACLKDPEGPS*PVSLLLAAQPHRQVAP AWGLFGMVQPGPPRKSAVEPR*LSWTARSMLPQ *ALLFEGLDGEVG*AEGTWQKREDFGHHGSAGL LQISAGLGWKQLCV*R*GLLEAGHPGQTP*QEGA HLSPLGSLRAGGDF*VLRG*RKKLSS*VPSQPP S*RCSEPHCPLSLCRWPVPLPALGALLAFYPIP GSTGAIEETQ*ECSILFIPRVAALGSGHSASGRE EEERGPIF*KTVQSLWLYVFMFFTCICVYVCIS FPPSIGTIFNKII*SK

Frame 1

FSQVPCMHIAADSTPAIAHNPQSQTL*PA*ERW SHLSVWRSWRMMCSNQRMGSR*PNPGACSLLTC LPRAYWTAPSADFSSSLSPTAVALAFDPPARKTK LPRLSAQPPPAKVSCCLVG*LRNPSDSFMAMLA IGFLSLPVSQQSCPLGSSPPKGSGNIKQRYRLP ESFSALQTSSTGFMCSNTSRTKIVCGGRSSSSCT TLL*MEKRTGTGQALGEGGLPSSHGAPGTPSGT GRTSGRTDTVSCEIVFLLLFCVLIFFNFSLSVH TTYSSGTFFLCQALDLEWGPLSNTVEGEADVSVM VRIPKGSDK*ILRLRNLPVVEDDPLVMFSGKWT VVFPEELS*EIESGLDSQFHQTYEPSTVDGGRD PDGHIALTQQGFGQGLST*G*QLH*AGRPAPHLT GFLLLSQYLPHGLKDSWPAPATSSRPQGGLSFS KDPSNMSQNEPNFWLGLKTDLVPLGGPRIGPEV QSHCHHWRPGTSWVSATDEPNSQSACCCWQPVPL SALLSKDPTDRPHSTPNIGTEGHRGQVCSGAGK YGGHPTMRVSRRGPQTPWFPWKQHTSSPLSPVL FHT*WDTEEARTVALPAK*LLE*QITIRVGNRQS WVHISYLPPASS*ARRACSGTQQDHSCLCTSSL LSLLRHLWRGSKAAGVAAPIAGTGNLLTRR*KL NYNFCICEKRQHWC*Q*SKAQRKDGFPGQRRPQG YPRK*EESRVDSQI*TSPSLPVLRRGGLQYY*R RH*SSGCTVVQVVHCTRAPAANGGWNSACVLLT KLCACPGVGLLLPRRRGAFPNLQRCHMGSQHPGG EGPQTVHVSLCFVLLRYSGYYLFLVRILFSGCV P*FVC*EDGFLLCREGAGDQLEDWLWDTLTIVK FSLPCGSPYHFMMCMGHTLFSPLWIKVVTGSLLP YASVAHTKKPGLFTAATIHPFWQTLHLSSVREK MI*ILVLGQGHQLQRPCQAQAGCPVA*ELSPDF DKPTSTSSLGQVEGGLTALSPSYAKPGTHSLS*V SQGKIYTPHVSLSLLPHQQNPGGSWQSSCQEGE RFFLAGCF*LA**SKLAPSSSAW*VGLNIPQV* PQETCPSPPTSTPSDSIPHPQEPGCL*RGVENQI *REGESDLDPDL*PAEDGGGFVAC*GWGLGEGL ETFLLGKEMPGRKGSLIFRVKTALLIHNPKAGV SRSWSTDLVREVPRPDAGLLQ*GWRTQPGLCLAC FKVWATGSLLLLNPRWKKITSQ*PPSAPRGVGL CPKLCGRLV*RTQRGPADLSHCSWQPSPIGRWP LPGGYSGWFSQGLPGRVLWSHGDCPGQQGACYPS EHFFLRDLMGRWGRQKGRGRSGKTLVTMALQAF CKSVLAWAGNSSVCEGEDSWKQAILARHPSRKG LTCHP*AA*GLVGTFES*GDSGKS*AHRCPASLP AKGAQSPTAPSLCAGGQCPSLLWEHC*PSTPSL DPQGLSRRPSENVAFCSSPG*LPWGLGTLPLGE RKKKGAPFFKKLYRAFGFMCLCSSHAYVCMCVYL SPHQLVQFLIKSFKA

Frame 2

FPRYPACI*LLILLLLLLTTLRVKLCDRPRRDG AISVCGGAGG*CVPTRGWGAGDPTRELALC*PV CPEPTGLPPQPTSALPSHPLLWPWPSTHQPGRQ SYPDSQPSLPQPRCHAALWGD*GTPATLLWQCWL SASSPCQFPSSLAHWGAAPRRAVATSSRGTDCP KASVHCRPVPPASCAATPAEPKSCVVADPPLPA QPCFEWRREQERGRP*VRVGCPLHMGHQEHRLEQ EGHRAGLTLCLVKLFFCCYFVF*FFLISL*VYI QHTQAGPSFSVRPLTWNGGLCQTLLKERLMCL* W*EFPRALTSRFFD*GIYQLSKMIR**CSLGSGL WFFQRNSVKKSRVD*TPSSTKPMSLPLWMGAVI LMVTLL*PSRASARGFPLEDSSFTRLAGQLHI* LGSYFSASTCPMGSRIPGQLLPPPADLREG*VSL RTPQTCRKMNQTSG*ASKLTWSHLEAPGLVLRY RATATTGGLGPAGCQPRMSRIASQHVAAGSLCL CQLFFQKTPPTDRIPPPTSALRDIGGKFAVGPEN MVATLP*ESAVGDPRPLGFLGNNIPLPPYPQSF FIPSGIQKKPGQWLCQLSDF*SNR*RLEWGTVK AGYTFPIFLQLPARPEGHALEPSRITAACARLPF SPCCGTYGEDLKQPVWQLR*QAQGIC*PDARN* IITFAYVRKDNIGANSEVRPKGKTASLDKEDLR ATQGNRRSLE*THKSEQAQVFQF*GEEVFSTIEG DIDLLDVQLCRLFTAQGHLQLMEAGIQLVFCSL SCVRALVWGYFSQEEGVPFLICKGAIWAHSTLE EKGLKLCM*AFVLFCSDILDITSSWSEFYSQGVY HDLSAKKTGSCFAEKEPGTSLKTGSGTH*PL*N SAFPVGLHTIL*CVWDIRCSPLSGSRW*QAACC RMLQWHTPKNRDYLQQPLFTPFGRLST*VQ*ERK *FRSWCWGKAISFRDLARPRLGALWPEN*AQTL TNPPQHQAWGRWKVV*LHCLHLMQSQELTHCLK *ARAKFTPLTFPSPFFPTNKTQEVAGRVAVRKEK GSFWLVVFNWLSDLNWPLPPLPGELA*TFLKSS LRRPAPPPRPPPPQTPSPTPRSLAVSSEGWRIR FRGRESLTWILTCNQLKTVEALWLAEGGGWERDW KPSSSGKKCLGGREA*YSGSKQPF*FTTPKQGF LEVGQQIW*GKSPGQTLDSCNEGGGLSLASAWP VSKSGPLAAFSCSIRGGKRSPPSDLPQPPGE*VC VQSYVAGLFEGPRGAQLTCLTAPGSPAPSAGGP CLGVIRDGSARASQEECCGATVTVLDSKEHATP VSTSF*GT*WGGGVGRRDVAEAGRLWSPWLCRPS ANQCWPGLETALCVKVRTLGSRPSWPDTLAGRG SPVTLRQPEGWWGLLSLEGIAEKAELIGAQPAS QLKVLRAPLPPLSVQVASAPPCSGSIASLLPHPW IHRGYRGDPVRM*HFVHPQGSCPGVWALCLWER GRRKGPHFLKNCTEPLALCVYVLHMHMCVCVYI FPPINWYNF**NHLKQ

Sequence BMF.cAug05

TAGCTGCTGATTCTACTCCTGCTATTGCTCACAACCCTCAGAGTCAAACTTTGTGACCGGCCTAGGTCAGAAAACGTGATCAAGAAAAAGGGTGGTTCCA GGCGGGCCCAGGGAAGAGGAGAGATGGAGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAACCAGAGGATGGGGAGCCGGTGACCCAACC CGGGAGCTTGCTCTCTGCTGACCTGTTTGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTTCCCTCTCACCCACTGCTGTGGCCCTGGC CTTCGACCCACCAGCCAGGAAGACAAAGCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATGCTGCCTTGTGGGGTGACTGAGGAACCCC AGCGACTCTTTTATGGCAATGCTGGCTATCGGCTTCCTCTCCCTGCCAGTTTCCCAGCAGTCTTGCCCATTGGGGAGCAGCCCCCCGAAGGGCAGTGGCA ACATCAAGCAGAGGTACAGATTGCCCGAAAGCTTCAGTGCATTGCAGACCAGTTCCACCGGCTTCATGTGCAGCAACACCAGCAGAACCAAAATCGTGTG TGGTGGCAGATCCTCCTCTTCCTGCACAACCTTGCTTTGAATGGAGAAGAGAACAGGAACGGGGCAGGCCCTAGGTGAGGGTGGGCTGCCCTCTTCACAT GGGGCACCAGGAACACCGTCTGGAACAGGAAGGACATCGGGCAGGACTGACACTGTGTCTTGTGAAATTGTTTTTTTGTTGTTATTTTGTGTTTTAATTT TTTTTAATTTCTCTCTGAGTGTACATACAACATACTCAAGCGGGACCTTCTTTCTCTGTCAGGCCCTTGACCTGGAATGGGGGCCTTTGTCAAACACTGT TGAAGGAGAGGCTGATGTGTCTGTGATGGTGAGAATTCCCAAGGGCTCTGACAAGTAGATTCTTCGACTGAGGAATCTACCAGTTGTCGAAGATGATCCG TTAGTGATGTTCTCTGGGAAGTGGACTGTGGTTTTTCCAGAGGAACTCAGTTAAGAAATCGAGAGTGGATTAGACTCCCAGTTCCACCAAACCTATGAGC CTTCCACTGTGGATGGGGGCCGTGATCCTGATGGTCACATTGCTTTAACCCAGCAGGGCTTCGGCCAGGGGCTTTCCACTTGAGGATAGCAGCTTCACTA GGCTGGCCGGCCAGCTCCACATCTGACTGGGTTCTTACTTCTCAGCCAGTACCTGCCCCATGGGCTCAAGGATTCCTGGCCAGCTCCTGCCACCTCCAGC AGACCTCAGGGAGGGTTGAGTTTCTCTAAGGACCCCTCAAACATGTCGCAAAATGAACCAAACTTCTGGCTAGGCCTCAAAACTGACTTGGTCCCACTTG GAGGCCCCAGGATTGGTCCTGAGGTACAGAGCCACTGCCACCACTGGCGGCCTGGGACCAGCTGGGTGTCAGCCACGGATGAGCCGAATAGCCAGTCAGC ATGTTGCTGCTGGCAGCCTGTGCCTTTGTCAGCTCTTCTTTCAAAAGACCCCACCGACAGACCGCATTCCACCCCCAACATCGGCACTGAGGGACATCGG GGGCAAGTTTGCAGTGGGGCCGGAAAATATGGTGGCCACCCTACCATGAGAGTCAGCCGTAGGGGACCCCAGACCCCTTGGTTTCCTTGGAAACAACATA CCTCTTCCCCCTTATCCCCAGTCCTTTTTCATACCTAGTGGGATACAGAAGAAGCCAGGACAGTGGCTTTGCCAGCTAAGTGACTTTTAGAGTAACAGAT AACGATTAGAGTGGGGAACCGTCAAAGCTGGGTACACATTTCCTATCTTCCTCCAGCTTCCAGCTAGGCCAGAAGGGCATGCTCTGGAACCCAGCAGGAT CACAGCTGCCTGTGCACGTCTTCCCTTCTCTCCCTGCTGCGGCACTTATGGAGAGGATCTAAAGCAGCCGGTGTGGCAGCTCCGATAGCAGGCACAGGGA ATCTGTTAACCAGACGCTAGAAACTAAATTATAACTTTTGCATATGTGAGAAAAGACAACATTGGTGCTAACAGTGAAGTAAGGCCCAAAGGAAAGACGG CTTCCCTGGACAAAGAAGACCTCAGGGCTACCCAAGGAAATAGGAGGAGTCTAGAGTAGACTCACAAATCTGAACAAGCCCAAGTCTTCCAGTTCTGAGG AGAGGAGGTCTTCAGTACTATTGAAGGAGACATTGATCTTCTGGATGTACAGTTGTGCAGGTTGTTCACTGCACAAGGGCACCTGCAGCTAATGGAGGCT GGAATTCAGCTTGTGTTCTGCTCACTAAGCTGTGTGCGTGCCCTGGTGTGGGGCTACTTCTCCCAAGAAGAAGGGGTGCCTTTCCTAATTTGCAAAGGTG CCATATGGGCTCACAGCACCCTGGAGGAGAAGGGCCTCAAACTGTGCATGTAAGCCTTTGTTTTGTTCTGCTCAGATATTCTGGATATTACCTCTTCTTG GTCAGAATTCTATTCTCAGGGTGTGTACCATGATTTGTCTGCTAAGAAGACGGGTTCCTGCTTTGCAGAGAAGGAGCCGGGGACCAGCTTGAAGACTGGC TCTGGGACACACTGACCATTGTGAAATTCAGCCTTCCCTGTGGGTCTCCATACCATTTTATGATGTGTATGGGACATACGTTGTTCTCCCCTCTCTGGAT CAAGGTGGTGACAGGCAGCCTGCTGCCGTATGCTTCAGTGGCACACACCAAAAAACCGGGATTATTTACAGCAGCCACTATTCACCCCTTTTGGCAGACT CTCCACCTGAGTTCAGTGAGAGAGAAAATGATTTAGATCTTGGTGCTGGGGCAAGGCCATCAGCTTCAGAGACCTTGCCAGGCCCAGGCTGGGTGCCCTG TGGCCTGAGAACTGAGCCCAGACTTTGACAAACCCACCTCAACATCAAGCCTGGGGCAGGTGGAAGGTGGTCTGACTGCACTGTCTCCATCTTATGCAAA GCCAGGAACTCACTCACTGTCTTAAGTGAGCCAGGGCAAAATTTACACCCCTCACGTTTCCCTCTCCCTTCTTCCCCACCAACAAAACCCAGGAGGTAGC TGGCAGAGTAGCTGTCAGGAAGGAGAAAGGTTCTTTCTGGCTGGTTGTTTTTAATTGGCTTAGTGATCTAAACTGGCCCCTTCCTCCTCTGCCTGGTGAG TTGGCCTAAACATTCCTCAAGTCTAGCCTCAGGAGACCTGCCCCTCCCCCCCGACCTCCACCCCCTCAGACTCCATCCCCCACCCCCAGGAGCCTGGCTG TCTCTAGCGAGGGGTGGAGAATCAGATTTAGAGGGAGGGAGAGTCTGACCTGGATCCTGACCTGTAACCAGCTGAAGACGGTGGAGGCTTTGTGGCTTGC TGAGGGTGGGGGTTGGGAGAGGGACTGGAAACCTTCCTCCTCGGGAAAGAAATGCCTGGGAGGAAGGGAAGCCTGATATTCAGGGTCAAAACAGCCCTTC TAATTCACAACCCCAAAGCAGGGGTTTCTAGAAGTTGGTCAACAGATCTGGTGAGGGAAGTCCCCAGGCCAGACGCTGGACTCCTGCAATGAGGGTGGAG GACTCAGCCTGGCCTCTGCCTGGCCTGTTTCAAAGTCTGGGCCACTGGCAGCCTTCTCTTGCTCAATCCGAGGTGGAAAAAGATCACCTCCCAGTGACCT CCCTCAGCCCCCAGGGGAGTAGGTCTGTGTCCAAAGCTATGTGGCAGGCTTGTTTGAAGGACCCAGAGGGGCCCAGCTGACCTGTCTCACTGCTCCTGGC AGCCCAGCCCCATCGGCAGGTGGCCCCTGCCTGGGGGTTATTCGGGATGGTTCAGCCAGGGCCTCCCAGGAAGAGTGCTGTGGAGCCACGGTGACTGTCC TGGACAGCAAGGAGCATGCTACCCCAGTGAGCACTTCTTTTTGAGGGACTTGATGGGGAGGTGGGGTAGGCAGAAGGGACGTGGCAGAAGCGGGAAGACT TTGGTCACCATGGCTCTGCAGGCCTTCTGCAAATCAGTGCTGGCCTGGGCTGGAAACAGCTCTGTGTGTGAAGGTGAGGACTCTTGGAAGCAGGCCATCC TGGCCAGACACCCTAGCAGGAAGGGGCTCACCTGTCACCCTTAGGCAGCCTGAGGGCTGGTGGGGACTTTTGAGTCTTGAGGGGATAGCGGAAAAAGCTG AGCTCATAGGTGCCCAGCCAGCCTCCCAGCTAAAGGTGCTCAGAGCCCCACTGCCCCCTCTCTCTGTGCAGGTGGCCAGTGCCCCTCCCTGCTCTGGGAG CATTGCTAGCCTTCTACCCCATCCCTGGATCCACAGGGGCTATCGAGGAGACCCAGTGAGAATGTAGCATTTTGTTCATCCCCAGGGTAGCTGCCCTGGG GTCTGGGCACTCTGCCTCTGGGAGAGAGGAAGAAGAAAGGGGCCCCATTTTTTAAAAAACTGTACAGAGCCTTTGGCTTTATGTGTTTATGTTCTTCACA TGCATATGTGTGTATGTGTGTATATCTTTCCCCCCATCAATTGGTACAATTTTTAATAAAATCATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Alternative donor

Frame 0

*LLILLLLLLTTLRVKLCDRPRSENVIKKKGGS RRAQGRGEMEPSQCVEELEDDVFQPEDGEPVTQP GSLLSADLFAQSLLDCPLSRLQLFPLTHCCGPG LRPTSQEDKATQTLSPASPSQGVMLPCGVTEEP QRLFYGNAGYRLPLPASFPAVLPIGEQPPEGQWQ HQAEVQIARKLQCIADQFHRLHVQQHQQNQNRV WWQILLFLHNLALNGEENRNGAGPR*GWAALFT WGTRNTVWNRKDIGQD*HCVL*NCFFVVILCFNF F*FLSECTYNILKRDLLSLSGP*PGMGAFVKHC *RRG*CVCDGENSQGL*QVDSSTEESTSCRR*S VSDVLWEVDCGFSRGTQLRNREWIRLPVPPNL*A FHCGWGP*S*WSHCFNPAGLRPGAFHLRIAASL GWPASSTSDWVLTSQPVPAPWAQGFLASSCHLQ QTSGRVEFL*GPLKHVAK*TKLLARPQN*LGPTW RPQDWS*GTEPLPPLAAWDQLGVSHG*AE*PVS MLLLAACAFVSSSFKRPHRQTAFHPQHRH*GTS GASLQWGRKIWWPPYHESQP*GTPDPLVSLETTY LFPLIPSPFSYLVGYRRSQDSGFAS*VTFRVTD ND*SGEPSKLGTHFLSSSSFQLGQKGMLWNPAG SQLPVHVFPSLPAAALMERI*SSRCGSSDSRHRE SVNQTLETKL*LLHM*EKTTLVLTVK*GPKERR LPWTKKTSGLPKEIGGV*SRLTNLNKPKSSSSE ERRSSVLLKETLIFWMYSCAGCSLHKGTCS*WRL EFSLCSAH*AVCVPWCGATSPKKKGCLS*FAKV PYGLTAPWRRRASNCACKPLFCSAQIFWILPLL GQNSILRVCTMICLLRRRVPALQRRSRGPA*RLA LGHTDHCEIQPSLWVSIPFYDVYGTYVVLPSLD QGGDRQPAAVCFSGTHQKTGIIYSSHYSPLLAD SPPEFSERENDLDLGAGARPSASETLPGPGWVPC GLRTEPRL*QTHLNIKPGAGGRWSDCTVSILCK ARNSLTVLSEPGQNLHPSRFPLPSSPPTKPRR* LAE*LSGRRKVLSGWLFLIGLVI*TGPFLLCLVS WPKHSSSLASGDLPLPPDLHPLRLHPPPPGAWL SLARGGESDLEGGRV*PGS*PVTS*RRWRLCGL LRVGVGRGTGNLPPRERNAWEEGKPDIQGQNSPS NSQPQSRGF*KLVNRSGEGSPQARRWTPAMRVE DSAWPLPGLFQSLGHWQPSLAQSEVEKDHLPVT SLSPQGSRSVSKAMWQACLKDPEGPS*PVSLLLA AQPHRQVAPAWGLFGMVQPGPPRKSAVEPR*LS WTARSMLPQ*ALLFEGLDGEVG*AEGTWQKRED FGHHGSAGLLQISAGLGWKQLCV*R*GLLEAGHP GQTP*QEGAHLSPLGSLRAGGDF*VLRG*RKKL SS*VPSQPPS*RCSEPHCPLSLCRWPVPLPALG ALLAFYPIPGSTGAIEETQ*ECSILFIPRVAALG SGHSASGREEEERGPIF*KTVQSLWLYVFMFFT CICVYVCISFPPSIGTIFNKII*SK

Frame 1

SC*FYSCYCSQPSESNFVTGLGQKT*SRKRVVP GGPREEERWSHLSVWRSWRMMCSNQRMGSR*PN PGACSLLTCLPRAYWTAPSADFSSSLSPTAVALA FDPPARKTKLPRLSAQPPPAKVSCCLVG*LRNP SDSFMAMLAIGFLSLPVSQQSCPLGSSPPKGSG NIKQRYRLPESFSALQTSSTGFMCSNTSRTKIVC GGRSSSSCTTLL*MEKRTGTGQALGEGGLPSSH GAPGTPSGTGRTSGRTDTVSCEIVFLLLFCVLI FFNFSLSVHTTYSSGTFFLCQALDLEWGPLSNTV EGEADVSVMVRIPKGSDK*ILRLRNLPVVEDDP LVMFSGKWTVVFPEELS*EIESGLDSQFHQTYE PSTVDGGRDPDGHIALTQQGFGQGLST*G*QLH* AGRPAPHLTGFLLLSQYLPHGLKDSWPAPATSS RPQGGLSFSKDPSNMSQNEPNFWLGLKTDLVPL GGPRIGPEVQSHCHHWRPGTSWVSATDEPNSQSA CCCWQPVPLSALLSKDPTDRPHSTPNIGTEGHR GQVCSGAGKYGGHPTMRVSRRGPQTPWFPWKQH TSSPLSPVLFHT*WDTEEARTVALPAK*LLE*QI TIRVGNRQSWVHISYLPPASS*ARRACSGTQQD HSCLCTSSLLSLLRHLWRGSKAAGVAAPIAGTG NLLTRR*KLNYNFCICEKRQHWC*Q*SKAQRKDG FPGQRRPQGYPRK*EESRVDSQI*TSPSLPVLR RGGLQYY*RRH*SSGCTVVQVVHCTRAPAANGG WNSACVLLTKLCACPGVGLLLPRRRGAFPNLQRC HMGSQHPGGEGPQTVHVSLCFVLLRYSGYYLFL VRILFSGCVP*FVC*EDGFLLCREGAGDQLEDW LWDTLTIVKFSLPCGSPYHFMMCMGHTLFSPLWI KVVTGSLLPYASVAHTKKPGLFTAATIHPFWQT LHLSSVREKMI*ILVLGQGHQLQRPCQAQAGCP VA*ELSPDFDKPTSTSSLGQVEGGLTALSPSYAK PGTHSLS*VSQGKIYTPHVSLSLLPHQQNPGGS WQSSCQEGERFFLAGCF*LA**SKLAPSSSAW* VGLNIPQV*PQETCPSPPTSTPSDSIPHPQEPGC L*RGVENQI*REGESDLDPDL*PAEDGGGFVAC *GWGLGEGLETFLLGKEMPGRKGSLIFRVKTAL LIHNPKAGVSRSWSTDLVREVPRPDAGLLQ*GWR TQPGLCLACFKVWATGSLLLLNPRWKKITSQ*P PSAPRGVGLCPKLCGRLV*RTQRGPADLSHCSW QPSPIGRWPLPGGYSGWFSQGLPGRVLWSHGDCP GQQGACYPSEHFFLRDLMGRWGRQKGRGRSGKT LVTMALQAFCKSVLAWAGNSSVCEGEDSWKQAI LARHPSRKGLTCHP*AA*GLVGTFES*GDSGKS* AHRCPASLPAKGAQSPTAPSLCAGGQCPSLLWE HC*PSTPSLDPQGLSRRPSENVAFCSSPG*LPW GLGTLPLGERKKKGAPFFKKLYRAFGFMCLCSSH AYVCMCVYLSPHQLVQFLIKSFKA

Frame 2

AADSTPAIAHNPQSQTL*PA*VRKRDQEKGWFQ AGPGKRRDGAISVCGGAGG*CVPTRGWGAGDPT RELALC*PVCPEPTGLPPQPTSALPSHPLLWPW PSTHQPGRQSYPDSQPSLPQPRCHAALWGD*GTP ATLLWQCWLSASSPCQFPSSLAHWGAAPRRAVA TSSRGTDCPKASVHCRPVPPASCAATPAEPKSC VVADPPLPAQPCFEWRREQERGRP*VRVGCPLHM GHQEHRLEQEGHRAGLTLCLVKLFFCCYFVF*F FLISL*VYIQHTQAGPSFSVRPLTWNGGLCQTL LKERLMCL*W*EFPRALTSRFFD*GIYQLSKMIR **CSLGSGLWFFQRNSVKKSRVD*TPSSTKPMS LPLWMGAVILMVTLL*PSRASARGFPLEDSSFT RLAGQLHI*LGSYFSASTCPMGSRIPGQLLPPPA DLREG*VSLRTPQTCRKMNQTSG*ASKLTWSHL EAPGLVLRYRATATTGGLGPAGCQPRMSRIASQ HVAAGSLCLCQLFFQKTPPTDRIPPPTSALRDIG GKFAVGPENMVATLP*ESAVGDPRPLGFLGNNI PLPPYPQSFFIPSGIQKKPGQWLCQLSDF*SNR *RLEWGTVKAGYTFPIFLQLPARPEGHALEPSRI TAACARLPFSPCCGTYGEDLKQPVWQLR*QAQG IC*PDARN*IITFAYVRKDNIGANSEVRPKGKT ASLDKEDLRATQGNRRSLE*THKSEQAQVFQF*G EEVFSTIEGDIDLLDVQLCRLFTAQGHLQLMEA GIQLVFCSLSCVRALVWGYFSQEEGVPFLICKG AIWAHSTLEEKGLKLCM*AFVLFCSDILDITSSW SEFYSQGVYHDLSAKKTGSCFAEKEPGTSLKTG SGTH*PL*NSAFPVGLHTIL*CVWDIRCSPLSG SRW*QAACCRMLQWHTPKNRDYLQQPLFTPFGRL ST*VQ*ERK*FRSWCWGKAISFRDLARPRLGAL WPEN*AQTLTNPPQHQAWGRWKVV*LHCLHLMQ SQELTHCLK*ARAKFTPLTFPSPFFPTNKTQEVA GRVAVRKEKGSFWLVVFNWLSDLNWPLPPLPGE LA*TFLKSSLRRPAPPPRPPPPQTPSPTPRSLA VSSEGWRIRFRGRESLTWILTCNQLKTVEALWLA EGGGWERDWKPSSSGKKCLGGREA*YSGSKQPF *FTTPKQGFLEVGQQIW*GKSPGQTLDSCNEGG GLSLASAWPVSKSGPLAAFSCSIRGGKRSPPSDL PQPPGE*VCVQSYVAGLFEGPRGAQLTCLTAPG SPAPSAGGPCLGVIRDGSARASQEECCGATVTV LDSKEHATPVSTSF*GT*WGGGVGRRDVAEAGRL WSPWLCRPSANQCWPGLETALCVKVRTLGSRPS WPDTLAGRGSPVTLRQPEGWWGLLSLEGIAEKA ELIGAQPASQLKVLRAPLPPLSVQVASAPPCSGS IASLLPHPWIHRGYRGDPVRM*HFVHPQGSCPG VWALCLWERGRRKGPHFLKNCTEPLALCVYVLH MHMCVCVYIFPPINWYNF**NHLKQ

Sequence BMF.aAug05

ATTTTGGAAACAATACCGCACCGTGCGGAGTGGCCTCCTCCCGCCCCGGCCTGTGCCCGCCGCCGCCGCCGCCCCTGCCTGCGCCTCCCGCCTCCTGCCG CAGCCCGCTGGGCTTTTTCCCTCCTTCCCAATCGAGTCTGGGCGTCCAGCCCCCGAGTGCTCGTCACGCTGGACCCTGGCGCGGAGCCCTGGCATCACGA CTCGGAGGCCGAGACTCTCTCCTGGAGTCACCCAGGAGAGATGGAGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAACCAGAGGATGGG GAGCCGGTGACCCAACCCGGGAGCTTGCTCTCTGCTGACCTGTTTGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTTCCCTCTCACCC ACTGCTGTGGCCCTGGCCTTCGACCCACCAGCCAGGAAGACAAAGCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATGCTGCCTTGTGG GGTGACTGAGGAACCCCAGCGACTCTTTTATGGCAATGCTGGCTATCGGCTTCCTCTCCCTGCCAGTTTCCCAGCAGTCTTGCCCATTGGGGAGCAGCCC CCCGAAGGGCAGTGGCAACATCAAGCAGAGGTACAGATTGCCCGAAAGCTTCAGTGCATTGCAGACCAGTTCCACCGGCTTCATGTGCAGCAACACCAGC AGAACCAAAATCGTGTGTGGTGGCAGATCCTCCTCTTCCTGCACAACCTTGCTTTGAATGGAGAAGAGAACAGGAACGGGGCAGGCCCTAGGTGAGGGTG GGCTGCCCTCTTCACATGGGGCACCAGGAACACCGTCTGGAACAGGAAGGACATCGGGCAGGACTGACACTGTGTCTTGTGAAATTGTTTTTTTGTTGTT ATTTTGTGTTTTAATTTTTTTTAATTTCTCTCTGAGTGTACATACAACATACTCAAGCGGGACCTTCTTTCTCTGTCAGGCCCTTGACCTGGAATGGGGG CCTTTGTCAAACACTGTTGAAGGAGAGGCTGATGTGTCTGTGATGGTGAGAATTCCCAAGGGCTCTGACAAGTAGATTCTTCGACTGAGGAATCTACCAG TTGTCGAAGATGATCCGTTAGTGATGTTCTCTGGGAAGTGGACTGTGGTTTTTCCAGAGGAACTCAGTTAAGAAATCGAGAGTGGATTAGACTCCCAGTT CCACCAAACCTATGAGCCTTCCACTGTGGATGGGGGCCGTGATCCTGATGGTCACATTGCTTTAACCCAGCAGGGCTTCGGCCAGGGGCTTTCCACTTGA GGATAGCAGCTTCACTAGGCTGGCCGGCCAGCTCCACATCTGACTGGGTTCTTACTTCTCAGCCAGTACCTGCCCCATGGGCTCAAGGATTCCTGGCCAG CTCCTGCCACCTCCAGCAGACCTCAGGGAGGGTTGAGTTTCTCTAAGGACCCCTCAAACATGTCGCAAAATGAACCAAACTTCTGGCTAGGCCTCAAAAC TGACTTGGTCCCACTTGGAGGCCCCAGGATTGGTCCTGAGGTACAGAGCCACTGCCACCACTGGCGGCCTGGGACCAGCTGGGTGTCAGCCACGGATGAG CCGAATAGCCAGTCAGCATGTTGCTGCTGGCAGCCTGTGCCTTTGTCAGCTCTTCTTTCAAAAGACCCCACCGACAGACCGCATTCCACCCCCAACATCG GCACTGAGGGACATCGGGGGCAAGTTTGCAGTGGGGCCGGAAAATATGGTGGCCACCCTACCATGAGAGTCAGCCGTAGGGGACCCCAGACCCCTTGGTT TCCTTGGAAACAACATACCTCTTCCCCCTTATCCCCAGTCCTTTTTCATACCTAGTGGGATACAGAAGAAGCCAGGACAGTGGCTTTGCCAGCTAAGTGA CTTTTAGAGTAACAGATAACGATTAGAGTGGGGAACCGTCAAAGCTGGGTACACATTTCCTATCTTCCTCCAGCTTCCAGCTAGGCCAGAAGGGCATGCT CTGGAACCCAGCAGGATCACAGCTGCCTGTGCACGTCTTCCCTTCTCTCCCTGCTGCGGCACTTATGGAGAGGATCTAAAGCAGCCGGTGTGGCAGCTCC GATAGCAGGCACAGGGAATCTGTTAACCAGACGCTAGAAACTAAATTATAACTTTTGCATATGTGAGAAAAGACAACATTGGTGCTAACAGTGAAGTAAG GCCCAAAGGAAAGACGGCTTCCCTGGACAAAGAAGACCTCAGGGCTACCCAAGGAAATAGGAGGAGTCTAGAGTAGACTCACAAATCTGAACAAGCCCAA GTCTTCCAGTTCTGAGGAGAGGAGGTCTTCAGTACTATTGAAGGAGACATTGATCTTCTGGATGTACAGTTGTGCAGGTTGTTCACTGCACAAGGGCACC TGCAGCTAATGGAGGCTGGAATTCAGCTTGTGTTCTGCTCACTAAGCTGTGTGCGTGCCCTGGTGTGGGGCTACTTCTCCCAAGAAGAAGGGGTGCCTTT CCTAATTTGCAAAGGTGCCATATGGGCTCACAGCACCCTGGAGGAGAAGGGCCTCAAACTGTGCATGTAAGCCTTTGTTTTGTTCTGCTCAGATATTCTG GATATTACCTCTTCTTGGTCAGAATTCTATTCTCAGGGTGTGTACCATGATTTGTCTGCTAAGAAGACGGGTTCCTGCTTTGCAGAGAAGGAGCCGGGGA CCAGCTTGAAGACTGGCTCTGGGACACACTGACCATTGTGAAATTCAGCCTTCCCTGTGGGTCTCCATACCATTTTATGATGTGTATGGGACATACGTTG TTCTCCCCTCTCTGGATCAAGGTGGTGACAGGCAGCCTGCTGCCGTATGCTTCAGTGGCACACACCAAAAAACCGGGATTATTTACAGCAGCCACTATTC ACCCCTTTTGGCAGACTCTCCACCTGAGTTCAGTGAGAGAGAAAATGATTTAGATCTTGGTGCTGGGGCAAGGCCATCAGCTTCAGAGACCTTGCCAGGC CCAGGCTGGGTGCCCTGTGGCCTGAGAACTGAGCCCAGACTTTGACAAACCCACCTCAACATCAAGCCTGGGGCAGGTGGAAGGTGGTCTGACTGCACTG TCTCCATCTTATGCAAAGCCAGGAACTCACTCACTGTCTTAAGTGAGCCAGGGCAAAATTTACACCCCTCACGTTTCCCTCTCCCTTCTTCCCCACCAAC AAAACCCAGGAGGTAGCTGGCAGAGTAGCTGTCAGGAAGGAGAAAGGTTCTTTCTGGCTGGTTGTTTTTAATTGGCTTAGTGATCTAAACTGGCCCCTTC CTCCTCTGCCTGGTGAGTTGGCCTAAACATTCCTCAAGTCTAGCCTCAGGAGACCTGCCCCTCCCCCCCGACCTCCACCCCCTCAGACTCCATCCCCCAC CCCCAGGAGCCTGGCTGTCTCTAGCGAGGGGTGGAGAATCAGATTTAGAGGGAGGGAGAGTCTGACCTGGATCCTGACCTGTAACCAGCTGAAGACGGTG GAGGCTTTGTGGCTTGCTGAGGGTGGGGGTTGGGAGAGGGACTGGAAACCTTCCTCCTCGGGAAAGAAATGCCTGGGAGGAAGGGAAGCCTGATATTCAG GGTCAAAACAGCCCTTCTAATTCACAACCCCAAAGCAGGGGTTTCTAGAAGTTGGTCAACAGATCTGGTGAGGGAAGTCCCCAGGCCAGACGCTGGACTC CTGCAATGAGGGTGGAGGACTCAGCCTGGCCTCTGCCTGGCCTGTTTCAAAGTCTGGGCCACTGGCAGCCTTCTCTTGCTCAATCCGAGGTGGAAAAAGA TCACCTCCCAGTGACCTCCCTCAGCCCCCAGGGGAGTAGGTCTGTGTCCAAAGCTATGTGGCAGGCTTGTTTGAAGGACCCAGAGGGGCCCAGCTGACCT GTCTCACTGCTCCTGGCAGCCCAGCCCCATCGGCAGGTGGCCCCTGCCTGGGGGTTATTCGGGATGGTTCAGCCAGGGCCTCCCAGGAAGAGTGCTGTGG AGCCACGGTGACTGTCCTGGACAGCAAGGAGCATGCTACCCCAGTGAGCACTTCTTTTTGAGGGACTTGATGGGGAGGTGGGGTAGGCAGAAGGGACGTG GCAGAAGCGGGAAGACTTTGGTCACCATGGCTCTGCAGGCCTTCTGCAAATCAGTGCTGGCCTGGGCTGGAAACAGCTCTGTGTGTGAAGGTGAGGACTC TTGGAAGCAGGCCATCCTGGCCAGACACCCTAGCAGGAAGGGGCTCACCTGTCACCCTTAGGCAGCCTGAGGGCTGGTGGGGACTTTTGAGTCTTGAGGG GATAGCGGAAAAAGCTGAGCTCATAGGTGCCCAGCCAGCCTCCCAGCTAAAGGTGCTCAGAGCCCCACTGCCCCCTCTCTCTGTGCAGGTGGCCAGTGCC CCTCCCTGCTCTGGGAGCATTGCTAGCCTTCTACCCCATCCCTGGATCCACAGGGGCTATCGAGGAGACCCAGTGAGAATGTAGCATTTTGTTCATCCCC AGGGTAGCTGCCCTGGGGTCTGGGCACTCTGCCTCTGGGAGAGAGGAAGAAGAAAGGGGCCCCATTTTTTAAAAAACTGTACAGAGCCTTTGGCTTTATG TGTTTATGTTCTTCACATGCATATGTGTGTATGTGTGTATATCTTTCCCCCCATCAATTGGTACAATTTTTAATAAAATCATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Alternative donor

Frame 0

ILETIPHRAEWPPPAPACARRRRRPCLRLPPPA AARWAFSLLPNRVWASSPRVLVTLDPGAEPWHHD SEAETLSWSHPGEMEPSQCVEELEDDVFQPEDG EPVTQPGSLLSADLFAQSLLDCPLSRLQLFPLT HCCGPGLRPTSQEDKATQTLSPASPSQGVMLPCG VTEEPQRLFYGNAGYRLPLPASFPAVLPIGEQP PEGQWQHQAEVQIARKLQCIADQFHRLHVQQHQ QNQNRVWWQILLFLHNLALNGEENRNGAGPR*GW AALFTWGTRNTVWNRKDIGQD*HCVL*NCFFVV ILCFNFF*FLSECTYNILKRDLLSLSGP*PGMG AFVKHC*RRG*CVCDGENSQGL*QVDSSTEESTS CRR*SVSDVLWEVDCGFSRGTQLRNREWIRLPV PPNL*AFHCGWGP*S*WSHCFNPAGLRPGAFHL RIAASLGWPASSTSDWVLTSQPVPAPWAQGFLAS SCHLQQTSGRVEFL*GPLKHVAK*TKLLARPQN *LGPTWRPQDWS*GTEPLPPLAAWDQLGVSHG* AE*PVSMLLLAACAFVSSSFKRPHRQTAFHPQHR H*GTSGASLQWGRKIWWPPYHESQP*GTPDPLV SLETTYLFPLIPSPFSYLVGYRRSQDSGFAS*V TFRVTDND*SGEPSKLGTHFLSSSSFQLGQKGML WNPAGSQLPVHVFPSLPAAALMERI*SSRCGSS DSRHRESVNQTLETKL*LLHM*EKTTLVLTVK* GPKERRLPWTKKTSGLPKEIGGV*SRLTNLNKPK SSSSEERRSSVLLKETLIFWMYSCAGCSLHKGT CS*WRLEFSLCSAH*AVCVPWCGATSPKKKGCL S*FAKVPYGLTAPWRRRASNCACKPLFCSAQIFW ILPLLGQNSILRVCTMICLLRRRVPALQRRSRG PA*RLALGHTDHCEIQPSLWVSIPFYDVYGTYV VLPSLDQGGDRQPAAVCFSGTHQKTGIIYSSHYS PLLADSPPEFSERENDLDLGAGARPSASETLPG PGWVPCGLRTEPRL*QTHLNIKPGAGGRWSDCT VSILCKARNSLTVLSEPGQNLHPSRFPLPSSPPT KPRR*LAE*LSGRRKVLSGWLFLIGLVI*TGPF LLCLVSWPKHSSSLASGDLPLPPDLHPLRLHPP PPGAWLSLARGGESDLEGGRV*PGS*PVTS*RRW RLCGLLRVGVGRGTGNLPPRERNAWEEGKPDIQ GQNSPSNSQPQSRGF*KLVNRSGEGSPQARRWT PAMRVEDSAWPLPGLFQSLGHWQPSLAQSEVEKD HLPVTSLSPQGSRSVSKAMWQACLKDPEGPS*P VSLLLAAQPHRQVAPAWGLFGMVQPGPPRKSAV EPR*LSWTARSMLPQ*ALLFEGLDGEVG*AEGTW QKREDFGHHGSAGLLQISAGLGWKQLCV*R*GL LEAGHPGQTP*QEGAHLSPLGSLRAGGDF*VLR G*RKKLSS*VPSQPPS*RCSEPHCPLSLCRWPVP LPALGALLAFYPIPGSTGAIEETQ*ECSILFIP RVAALGSGHSASGREEEERGPIF*KTVQSLWLY VFMFFTCICVYVCISFPPSIGTIFNKII*SK

Frame 1

FWKQYRTVRSGLLPPRPVPAAAAAPACASRLLP QPAGLFPSFPIESGRPAPECSSRWTLARSPGIT TRRPRLSPGVTQERWSHLSVWRSWRMMCSNQRMG SR*PNPGACSLLTCLPRAYWTAPSADFSSSLSP TAVALAFDPPARKTKLPRLSAQPPPAKVSCCLV G*LRNPSDSFMAMLAIGFLSLPVSQQSCPLGSSP PKGSGNIKQRYRLPESFSALQTSSTGFMCSNTS RTKIVCGGRSSSSCTTLL*MEKRTGTGQALGEG GLPSSHGAPGTPSGTGRTSGRTDTVSCEIVFLLL FCVLIFFNFSLSVHTTYSSGTFFLCQALDLEWG PLSNTVEGEADVSVMVRIPKGSDK*ILRLRNLP VVEDDPLVMFSGKWTVVFPEELS*EIESGLDSQF HQTYEPSTVDGGRDPDGHIALTQQGFGQGLST* G*QLH*AGRPAPHLTGFLLLSQYLPHGLKDSWP APATSSRPQGGLSFSKDPSNMSQNEPNFWLGLKT DLVPLGGPRIGPEVQSHCHHWRPGTSWVSATDE PNSQSACCCWQPVPLSALLSKDPTDRPHSTPNI GTEGHRGQVCSGAGKYGGHPTMRVSRRGPQTPWF PWKQHTSSPLSPVLFHT*WDTEEARTVALPAK* LLE*QITIRVGNRQSWVHISYLPPASS*ARRAC SGTQQDHSCLCTSSLLSLLRHLWRGSKAAGVAAP IAGTGNLLTRR*KLNYNFCICEKRQHWC*Q*SK AQRKDGFPGQRRPQGYPRK*EESRVDSQI*TSP SLPVLRRGGLQYY*RRH*SSGCTVVQVVHCTRAP AANGGWNSACVLLTKLCACPGVGLLLPRRRGAF PNLQRCHMGSQHPGGEGPQTVHVSLCFVLLRYS GYYLFLVRILFSGCVP*FVC*EDGFLLCREGAGD QLEDWLWDTLTIVKFSLPCGSPYHFMMCMGHTL FSPLWIKVVTGSLLPYASVAHTKKPGLFTAATI HPFWQTLHLSSVREKMI*ILVLGQGHQLQRPCQA QAGCPVA*ELSPDFDKPTSTSSLGQVEGGLTAL SPSYAKPGTHSLS*VSQGKIYTPHVSLSLLPHQ QNPGGSWQSSCQEGERFFLAGCF*LA**SKLAPS SSAW*VGLNIPQV*PQETCPSPPTSTPSDSIPH PQEPGCL*RGVENQI*REGESDLDPDL*PAEDG GGFVAC*GWGLGEGLETFLLGKEMPGRKGSLIFR VKTALLIHNPKAGVSRSWSTDLVREVPRPDAGL LQ*GWRTQPGLCLACFKVWATGSLLLLNPRWKK ITSQ*PPSAPRGVGLCPKLCGRLV*RTQRGPADL SHCSWQPSPIGRWPLPGGYSGWFSQGLPGRVLW SHGDCPGQQGACYPSEHFFLRDLMGRWGRQKGR GRSGKTLVTMALQAFCKSVLAWAGNSSVCEGEDS WKQAILARHPSRKGLTCHP*AA*GLVGTFES*G DSGKS*AHRCPASLPAKGAQSPTAPSLCAGGQC PSLLWEHC*PSTPSLDPQGLSRRPSENVAFCSSP G*LPWGLGTLPLGERKKKGAPFFKKLYRAFGFM CLCSSHAYVCMCVYLSPHQLVQFLIKSFKA

Frame 2

FGNNTAPCGVASSRPGLCPPPPPPLPAPPASCR SPLGFFPPSQSSLGVQPPSARHAGPWRGALASR LGGRDSLLESPRRDGAISVCGGAGG*CVPTRGW GAGDPTRELALC*PVCPEPTGLPPQPTSALPSHP LLWPWPSTHQPGRQSYPDSQPSLPQPRCHAALW GD*GTPATLLWQCWLSASSPCQFPSSLAHWGAA PRRAVATSSRGTDCPKASVHCRPVPPASCAATPA EPKSCVVADPPLPAQPCFEWRREQERGRP*VRV GCPLHMGHQEHRLEQEGHRAGLTLCLVKLFFCC YFVF*FFLISL*VYIQHTQAGPSFSVRPLTWNGG LCQTLLKERLMCL*W*EFPRALTSRFFD*GIYQ LSKMIR**CSLGSGLWFFQRNSVKKSRVD*TPS STKPMSLPLWMGAVILMVTLL*PSRASARGFPLE DSSFTRLAGQLHI*LGSYFSASTCPMGSRIPGQ LLPPPADLREG*VSLRTPQTCRKMNQTSG*ASK LTWSHLEAPGLVLRYRATATTGGLGPAGCQPRMS RIASQHVAAGSLCLCQLFFQKTPPTDRIPPPTS ALRDIGGKFAVGPENMVATLP*ESAVGDPRPLG FLGNNIPLPPYPQSFFIPSGIQKKPGQWLCQLSD F*SNR*RLEWGTVKAGYTFPIFLQLPARPEGHA LEPSRITAACARLPFSPCCGTYGEDLKQPVWQL R*QAQGIC*PDARN*IITFAYVRKDNIGANSEVR PKGKTASLDKEDLRATQGNRRSLE*THKSEQAQ VFQF*GEEVFSTIEGDIDLLDVQLCRLFTAQGH LQLMEAGIQLVFCSLSCVRALVWGYFSQEEGVPF LICKGAIWAHSTLEEKGLKLCM*AFVLFCSDIL DITSSWSEFYSQGVYHDLSAKKTGSCFAEKEPG TSLKTGSGTH*PL*NSAFPVGLHTIL*CVWDIRC SPLSGSRW*QAACCRMLQWHTPKNRDYLQQPLF TPFGRLST*VQ*ERK*FRSWCWGKAISFRDLAR PRLGALWPEN*AQTLTNPPQHQAWGRWKVV*LHC LHLMQSQELTHCLK*ARAKFTPLTFPSPFFPTN KTQEVAGRVAVRKEKGSFWLVVFNWLSDLNWPL PPLPGELA*TFLKSSLRRPAPPPRPPPPQTPSPT PRSLAVSSEGWRIRFRGRESLTWILTCNQLKTV EALWLAEGGGWERDWKPSSSGKKCLGGREA*YS GSKQPF*FTTPKQGFLEVGQQIW*GKSPGQTLDS CNEGGGLSLASAWPVSKSGPLAAFSCSIRGGKR SPPSDLPQPPGE*VCVQSYVAGLFEGPRGAQLT CLTAPGSPAPSAGGPCLGVIRDGSARASQEECCG ATVTVLDSKEHATPVSTSF*GT*WGGGVGRRDV AEAGRLWSPWLCRPSANQCWPGLETALCVKVRT LGSRPSWPDTLAGRGSPVTLRQPEGWWGLLSLEG IAEKAELIGAQPASQLKVLRAPLPPLSVQVASA PPCSGSIASLLPHPWIHRGYRGDPVRM*HFVHP QGSCPGVWALCLWERGRRKGPHFLKNCTEPLALC VYVLHMHMCVCVYIFPPINWYNF**NHLKQ