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Sequences

Sequence BMF.eAug05

ATTTTGGAAACAATACCGCACCGTGCGGAGTGGCCTCCTCCCGCCCCGGCCTGTGCCCGCCGCCGCCGCCGCCCCTGCCTGCGCCTCCCGCCTCCTGCCG CAGCCCGCTGGGCTTTTTCCCTCCTTCCCAATCGAGTCTGGGCGTCCAGCCCCCGAGTGCTCGTCACGCTGGACCCTGGCGCGGAGCCCTGGCATCACGA CTCGGAGGCCGAGACTCTCTCCTGGAGTCACCCAGGTACCCTGCATGCATATAGCTGCTGATTCTACTCCTGCTATTGCTCACAACCCTCAGAGTCAAAC TTTGTGACCGGCCTAGGAGAGATGGAGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAACCAGAGGATGGGGAGCCGGTGACCCAACCCG GGAGCTTGCTCTCTGCTGACCTGTTTGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTTCCCTCTCACCCACTGCTGTGGCCCTGGCCT TCGACCCACCAGCCAGGAAGACAAAGCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATGCTGCCTTGTGGGGTGACTGAGGAACCCCAG CGACTCTTTTATGCACCAGCAGAACCAAAATCGTGTGTGGTGGCAGATCCTCCTCTTCCTGCACAACCTTGCTTTGAATGGAGAAGAGAACAGGAACGGG GCAGGCCCTAGGTGAGGGTGGGCTGCCCTCTTCACATGGGGCACCAGGAACACCGTCTGGAACAGGAAGGACATCGGGCAGGACTGACACTGTGTCTTGT GAAATTGTTTTTTTGTTGTTATTTTGTGTTTTAATTTTTTTTAATTTCTCTCTGAGTGTACATACAACATACTCAAGCGGGACCTTCTTTCTCTGTCAGG CCCTTGACCTGGAATGGGGGCCTTTGTCAAACACTGTTGAAGGAGAGGCTGATGTGTCTGTGATGGTGAGAATTCCCAAGGGCTCTGACAAGTAGATTCT TCGACTGAGGAATCTACCAGTTGTCGAAGATGATCCGTTAGTGATGTTCTCTGGGAAGTGGACTGTGGTTTTTCCAGAGGAACTCAGTTAAGAAATCGAG AGTGGATTAGACTCCCAGTTCCACCAAACCTATGAGCCTTCCACTGTGGATGGGGGCCGTGATCCTGATGGTCACATTGCTTTAACCCAGCAGGGCTTCG GCCAGGGGCTTTCCACTTGAGGATAGCAGCTTCACTAGGCTGGCCGGCCAGCTCCACATCTGACTGGGTTCTTACTTCTCAGCCAGTACCTGCCCCATGG GCTCAAGGATTCCTGGCCAGCTCCTGCCACCTCCAGCAGACCTCAGGGAGGGTTGAGTTTCTCTAAGGACCCCTCAAACATGTCGCAAAATGAACCAAAC TTCTGGCTAGGCCTCAAAACTGACTTGGTCCCACTTGGAGGCCCCAGGATTGGTCCTGAGGTACAGAGCCACTGCCACCACTGGCGGCCTGGGACCAGCT GGGTGTCAGCCACGGATGAGCCGAATAGCCAGTCAGCATGTTGCTGCTGGCAGCCTGTGCCTTTGTCAGCTCTTCTTTCAAAAGACCCCACCGACAGACC GCATTCCACCCCCAACATCGGCACTGAGGGACATCGGGGGCAAGTTTGCAGTGGGGCCGGAAAATATGGTGGCCACCCTACCATGAGAGTCAGCCGTAGG GGACCCCAGACCCCTTGGTTTCCTTGGAAACAACATACCTCTTCCCCCTTATCCCCAGTCCTTTTTCATACCTAGTGGGATACAGAAGAAGCCAGGACAG TGGCTTTGCCAGCTAAGTGACTTTTAGAGTAACAGATAACGATTAGAGTGGGGAACCGTCAAAGCTGGGTACACATTTCCTATCTTCCTCCAGCTTCCAG CTAGGCCAGAAGGGCATGCTCTGGAACCCAGCAGGATCACAGCTGCCTGTGCACGTCTTCCCTTCTCTCCCTGCTGCGGCACTTATGGAGAGGATCTAAA GCAGCCGGTGTGGCAGCTCCGATAGCAGGCACAGGGAATCTGTTAACCAGACGCTAGAAACTAAATTATAACTTTTGCATATGTGAGAAAAGACAACATT GGTGCTAACAGTGAAGTAAGGCCCAAAGGAAAGACGGCTTCCCTGGACAAAGAAGACCTCAGGGCTACCCAAGGAAATAGGAGGAGTCTAGAGTAGACTC ACAAATCTGAACAAGCCCAAGTCTTCCAGTTCTGAGGAGAGGAGGTCTTCAGTACTATTGAAGGAGACATTGATCTTCTGGATGTACAGTTGTGCAGGTT GTTCACTGCACAAGGGCACCTGCAGCTAATGGAGGCTGGAATTCAGCTTGTGTTCTGCTCACTAAGCTGTGTGCGTGCCCTGGTGTGGGGCTACTTCTCC CAAGAAGAAGGGGTGCCTTTCCTAATTTGCAAAGGTGCCATATGGGCTCACAGCACCCTGGAGGAGAAGGGCCTCAAACTGTGCATGTAAGCCTTTGTTT TGTTCTGCTCAGATATTCTGGATATTACCTCTTCTTGGTCAGAATTCTATTCTCAGGGTGTGTACCATGATTTGTCTGCTAAGAAGACGGGTTCCTGCTT TGCAGAGAAGGAGCCGGGGACCAGCTTGAAGACTGGCTCTGGGACACACTGACCATTGTGAAATTCAGCCTTCCCTGTGGGTCTCCATACCATTTTATGA TGTGTATGGGACATACGTTGTTCTCCCCTCTCTGGATCAAGGTGGTGACAGGCAGCCTGCTGCCGTATGCTTCAGTGGCACACACCAAAAAACCGGGATT ATTTACAGCAGCCACTATTCACCCCTTTTGGCAGACTCTCCACCTGAGTTCAGTGAGAGAGAAAATGATTTAGATCTTGGTGCTGGGGCAAGGCCATCAG CTTCAGAGACCTTGCCAGGCCCAGGCTGGGTGCCCTGTGGCCTGAGAACTGAGCCCAGACTTTGACAAACCCACCTCAACATCAAGCCTGGGGCAGGTGG AAGGTGGTCTGACTGCACTGTCTCCATCTTATGCAAAGCCAGGAACTCACTCACTGTCTTAAGTGAGCCAGGGCAAAATTTACACCCCTCACGTTTCCCT CTCCCTTCTTCCCCACCAACAAAACCCAGGAGGTAGCTGGCAGAGTAGCTGTCAGGAAGGAGAAAGGTTCTTTCTGGCTGGTTGTTTTTAATTGGCTTAG TGATCTAAACTGGCCCCTTCCTCCTCTGCCTGGTGAGTTGGCCTAAACATTCCTCAAGTCTAGCCTCAGGAGACCTGCCCCTCCCCCCCGACCTCCACCC CCTCAGACTCCATCCCCCACCCCCAGGAGCCTGGCTGTCTCTAGCGAGGGGTGGAGAATCAGATTTAGAGGGAGGGAGAGTCTGACCTGGATCCTGACCT GTAACCAGCTGAAGACGGTGGAGGCTTTGTGGCTTGCTGAGGGTGGGGGTTGGGAGAGGGACTGGAAACCTTCCTCCTCGGGAAAGAAATGCCTGGGAGG AAGGGAAGCCTGATATTCAGGGTCAAAACAGCCCTTCTAATTCACAACCCCAAAGCAGGGGTTTCTAGAAGTTGGTCAACAGATCTGGTGAGGGAAGTCC CCAGGCCAGACGCTGGACTCCTGCAATGAGGGTGGAGGACTCAGCCTGGCCTCTGCCTGGCCTGTTTCAAAGTCTGGGCCACTGGCAGCCTTCTCTTGCT CAATCCGAGGTGGAAAAAGATCACCTCCCAGTGACCTCCCTCAGCCCCCAGGGGAGTAGGTCTGTGTCCAAAGCTATGTGGCAGGCTTGTTTGAAGGACC CAGAGGGGCCCAGCTGACCTGTCTCACTGCTCCTGGCAGCCCAGCCCCATCGGCAGGTGGCCCCTGCCTGGGGGTTATTCGGGATGGTTCAGCCAGGGCC TCCCAGGAAGAGTGCTGTGGAGCCACGGTGACTGTCCTGGACAGCAAGGAGCATGCTACCCCAGTGAGCACTTCTTTTTGAGGGACTTGATGGGGAGGTG GGGTAGGCAGAAGGGACGTGGCAGAAGCGGGAAGACTTTGGTCACCATGGCTCTGCAGGCCTTCTGCAAATCAGTGCTGGCCTGGGCTGGAAACAGCTCT GTGTGTGAAGGTGAGGACTCTTGGAAGCAGGCCATCCTGGCCAGACACCCTAGCAGGAAGGGGCTCACCTGTCACCCTTAGGCAGCCTGAGGGCTGGTGG GGACTTTTGAGTCTTGAGGGGATAGCGGAAAAAGCTGAGCTCATAGGTGCCCAGCCAGCCTCCCAGCTAAAGGTGCTCAGAGCCCCACTGCCCCCTCTCT CTGTGCAGGTGGCCAGTGCCCCTCCCTGCTCTGGGAGCATTGCTAGCCTTCTACCCCATCCCTGGATCCACAGGGGCTATCGAGGAGACCCAGTGAGAAT GTAGCATTTTGTTCATCCCCAGGGTAGCTGCCCTGGGGTCTGGGCACTCTGCCTCTGGGAGAGAGGAAGAAGAAAGGGGCCCCATTTTTTAAAAAACTGT ACAGAGCCTTTGGCTTTATGTGTTTATGTTCTTCACATGCATATGTGTGTATGTGTGTATATCTTTCCCCCCATCAATTGGTACAATTTTTAATAAAATC ATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Single exon skipping

Frame 0

ILETIPHRAEWPPPAPACARRRRRPCLRLPPPA AARWAFSLLPNRVWASSPRVLVTLDPGAEPWHHD SEAETLSWSHPGTLHAYSC*FYSCYCSQPSESN FVTGLGEMEPSQCVEELEDDVFQPEDGEPVTQP GSLLSADLFAQSLLDCPLSRLQLFPLTHCCGPGL RPTSQEDKATQTLSPASPSQGVMLPCGVTEEPQ RLFYAPAEPKSCVVADPPLPAQPCFEWRREQER GRP*VRVGCPLHMGHQEHRLEQEGHRAGLTLCLV KLFFCCYFVF*FFLISL*VYIQHTQAGPSFSVR PLTWNGGLCQTLLKERLMCL*W*EFPRALTSRF FD*GIYQLSKMIR**CSLGSGLWFFQRNSVKKSR VD*TPSSTKPMSLPLWMGAVILMVTLL*PSRAS ARGFPLEDSSFTRLAGQLHI*LGSYFSASTCPM GSRIPGQLLPPPADLREG*VSLRTPQTCRKMNQT SG*ASKLTWSHLEAPGLVLRYRATATTGGLGPA GCQPRMSRIASQHVAAGSLCLCQLFFQKTPPTD RIPPPTSALRDIGGKFAVGPENMVATLP*ESAVG DPRPLGFLGNNIPLPPYPQSFFIPSGIQKKPGQ WLCQLSDF*SNR*RLEWGTVKAGYTFPIFLQLP ARPEGHALEPSRITAACARLPFSPCCGTYGEDLK QPVWQLR*QAQGIC*PDARN*IITFAYVRKDNI GANSEVRPKGKTASLDKEDLRATQGNRRSLE*T HKSEQAQVFQF*GEEVFSTIEGDIDLLDVQLCRL FTAQGHLQLMEAGIQLVFCSLSCVRALVWGYFS QEEGVPFLICKGAIWAHSTLEEKGLKLCM*AFV LFCSDILDITSSWSEFYSQGVYHDLSAKKTGSCF AEKEPGTSLKTGSGTH*PL*NSAFPVGLHTIL* CVWDIRCSPLSGSRW*QAACCRMLQWHTPKNRD YLQQPLFTPFGRLST*VQ*ERK*FRSWCWGKAIS FRDLARPRLGALWPEN*AQTLTNPPQHQAWGRW KVV*LHCLHLMQSQELTHCLK*ARAKFTPLTFP SPFFPTNKTQEVAGRVAVRKEKGSFWLVVFNWLS DLNWPLPPLPGELA*TFLKSSLRRPAPPPRPPP PQTPSPTPRSLAVSSEGWRIRFRGRESLTWILT CNQLKTVEALWLAEGGGWERDWKPSSSGKKCLGG REA*YSGSKQPF*FTTPKQGFLEVGQQIW*GKS PGQTLDSCNEGGGLSLASAWPVSKSGPLAAFSC SIRGGKRSPPSDLPQPPGE*VCVQSYVAGLFEGP RGAQLTCLTAPGSPAPSAGGPCLGVIRDGSARA SQEECCGATVTVLDSKEHATPVSTSF*GT*WGG GVGRRDVAEAGRLWSPWLCRPSANQCWPGLETAL CVKVRTLGSRPSWPDTLAGRGSPVTLRQPEGWW GLLSLEGIAEKAELIGAQPASQLKVLRAPLPPL SVQVASAPPCSGSIASLLPHPWIHRGYRGDPVRM *HFVHPQGSCPGVWALCLWERGRRKGPHFLKNC TEPLALCVYVLHMHMCVCVYIFPPINWYNF**N HLKQ

Frame 1

FWKQYRTVRSGLLPPRPVPAAAAAPACASRLLP QPAGLFPSFPIESGRPAPECSSRWTLARSPGIT TRRPRLSPGVTQVPCMHIAADSTPAIAHNPQSQT L*PA*ERWSHLSVWRSWRMMCSNQRMGSR*PNP GACSLLTCLPRAYWTAPSADFSSSLSPTAVALA FDPPARKTKLPRLSAQPPPAKVSCCLVG*LRNPS DSFMHQQNQNRVWWQILLFLHNLALNGEENRNG AGPR*GWAALFTWGTRNTVWNRKDIGQD*HCVL *NCFFVVILCFNFF*FLSECTYNILKRDLLSLSG P*PGMGAFVKHC*RRG*CVCDGENSQGL*QVDS STEESTSCRR*SVSDVLWEVDCGFSRGTQLRNR EWIRLPVPPNL*AFHCGWGP*S*WSHCFNPAGLR PGAFHLRIAASLGWPASSTSDWVLTSQPVPAPW AQGFLASSCHLQQTSGRVEFL*GPLKHVAK*TK LLARPQN*LGPTWRPQDWS*GTEPLPPLAAWDQL GVSHG*AE*PVSMLLLAACAFVSSSFKRPHRQT AFHPQHRH*GTSGASLQWGRKIWWPPYHESQP* GTPDPLVSLETTYLFPLIPSPFSYLVGYRRSQDS GFAS*VTFRVTDND*SGEPSKLGTHFLSSSSFQ LGQKGMLWNPAGSQLPVHVFPSLPAAALMERI* SSRCGSSDSRHRESVNQTLETKL*LLHM*EKTTL VLTVK*GPKERRLPWTKKTSGLPKEIGGV*SRL TNLNKPKSSSSEERRSSVLLKETLIFWMYSCAG CSLHKGTCS*WRLEFSLCSAH*AVCVPWCGATSP KKKGCLS*FAKVPYGLTAPWRRRASNCACKPLF CSAQIFWILPLLGQNSILRVCTMICLLRRRVPA LQRRSRGPA*RLALGHTDHCEIQPSLWVSIPFYD VYGTYVVLPSLDQGGDRQPAAVCFSGTHQKTGI IYSSHYSPLLADSPPEFSERENDLDLGAGARPS ASETLPGPGWVPCGLRTEPRL*QTHLNIKPGAGG RWSDCTVSILCKARNSLTVLSEPGQNLHPSRFP LPSSPPTKPRR*LAE*LSGRRKVLSGWLFLIGL VI*TGPFLLCLVSWPKHSSSLASGDLPLPPDLHP LRLHPPPPGAWLSLARGGESDLEGGRV*PGS*P VTS*RRWRLCGLLRVGVGRGTGNLPPRERNAWE EGKPDIQGQNSPSNSQPQSRGF*KLVNRSGEGSP QARRWTPAMRVEDSAWPLPGLFQSLGHWQPSLA QSEVEKDHLPVTSLSPQGSRSVSKAMWQACLKD PEGPS*PVSLLLAAQPHRQVAPAWGLFGMVQPGP PRKSAVEPR*LSWTARSMLPQ*ALLFEGLDGEV G*AEGTWQKREDFGHHGSAGLLQISAGLGWKQL CV*R*GLLEAGHPGQTP*QEGAHLSPLGSLRAGG DF*VLRG*RKKLSS*VPSQPPS*RCSEPHCPLS LCRWPVPLPALGALLAFYPIPGSTGAIEETQ*E CSILFIPRVAALGSGHSASGREEEERGPIF*KTV QSLWLYVFMFFTCICVYVCISFPPSIGTIFNKI I*SK

Frame 2

FGNNTAPCGVASSRPGLCPPPPPPLPAPPASCR SPLGFFPPSQSSLGVQPPSARHAGPWRGALASR LGGRDSLLESPRYPACI*LLILLLLLLTTLRVK LCDRPRRDGAISVCGGAGG*CVPTRGWGAGDPTR ELALC*PVCPEPTGLPPQPTSALPSHPLLWPWP STHQPGRQSYPDSQPSLPQPRCHAALWGD*GTP ATLLCTSRTKIVCGGRSSSSCTTLL*MEKRTGTG QALGEGGLPSSHGAPGTPSGTGRTSGRTDTVSC EIVFLLLFCVLIFFNFSLSVHTTYSSGTFFLCQ ALDLEWGPLSNTVEGEADVSVMVRIPKGSDK*IL RLRNLPVVEDDPLVMFSGKWTVVFPEELS*EIE SGLDSQFHQTYEPSTVDGGRDPDGHIALTQQGF GQGLST*G*QLH*AGRPAPHLTGFLLLSQYLPHG LKDSWPAPATSSRPQGGLSFSKDPSNMSQNEPN FWLGLKTDLVPLGGPRIGPEVQSHCHHWRPGTS WVSATDEPNSQSACCCWQPVPLSALLSKDPTDRP HSTPNIGTEGHRGQVCSGAGKYGGHPTMRVSRR GPQTPWFPWKQHTSSPLSPVLFHT*WDTEEART VALPAK*LLE*QITIRVGNRQSWVHISYLPPASS *ARRACSGTQQDHSCLCTSSLLSLLRHLWRGSK AAGVAAPIAGTGNLLTRR*KLNYNFCICEKRQH WC*Q*SKAQRKDGFPGQRRPQGYPRK*EESRVDS QI*TSPSLPVLRRGGLQYY*RRH*SSGCTVVQV VHCTRAPAANGGWNSACVLLTKLCACPGVGLLL PRRRGAFPNLQRCHMGSQHPGGEGPQTVHVSLCF VLLRYSGYYLFLVRILFSGCVP*FVC*EDGFLL CREGAGDQLEDWLWDTLTIVKFSLPCGSPYHFM MCMGHTLFSPLWIKVVTGSLLPYASVAHTKKPGL FTAATIHPFWQTLHLSSVREKMI*ILVLGQGHQ LQRPCQAQAGCPVA*ELSPDFDKPTSTSSLGQV EGGLTALSPSYAKPGTHSLS*VSQGKIYTPHVSL SLLPHQQNPGGSWQSSCQEGERFFLAGCF*LA* *SKLAPSSSAW*VGLNIPQV*PQETCPSPPTST PSDSIPHPQEPGCL*RGVENQI*REGESDLDPDL *PAEDGGGFVAC*GWGLGEGLETFLLGKEMPGR KGSLIFRVKTALLIHNPKAGVSRSWSTDLVREV PRPDAGLLQ*GWRTQPGLCLACFKVWATGSLLLL NPRWKKITSQ*PPSAPRGVGLCPKLCGRLV*RT QRGPADLSHCSWQPSPIGRWPLPGGYSGWFSQG LPGRVLWSHGDCPGQQGACYPSEHFFLRDLMGRW GRQKGRGRSGKTLVTMALQAFCKSVLAWAGNSS VCEGEDSWKQAILARHPSRKGLTCHP*AA*GLV GTFES*GDSGKS*AHRCPASLPAKGAQSPTAPSL CAGGQCPSLLWEHC*PSTPSLDPQGLSRRPSEN VAFCSSPG*LPWGLGTLPLGERKKKGAPFFKKL YRAFGFMCLCSSHAYVCMCVYLSPHQLVQFLIKS FKA

Sequence BMF.bAug05

GGAGTCGCGCTGCGCACGGGGGCCCGGGAAGGGTTTGGGCTGCGGACGTCTGGGGTGACCATGAAGAACTGGGGCGCTGGGGTGAGGGAACGGGGTGCAG AACCCACAGTTGGGTGCGCTGCTCCAAGAGGGAACTTGGGGGTAGCATTTGGGACTCGAAGGAGTTGGGTCCAGGTAGGCAGGGGAACCTGGGGTGCACT GCTGAGCAGACCTGGGCGCCCGAGAACGGCCCAGCCTCTGGGTCTGACTTTGGGATAGGACAGGAGCTGTGGGGCATCCTGCCTCTCTGCGGAGTCTGAA GGCTCCTCTCCTTAACAGCTGGGCTTTTTCCCTCCTTCCCAATCGAGTCTGGGCGTCCAGCCCCCGAGTGCTCGTCACGCTGGACCCTGGCGCGGAGCCC TGGCATCACGACTCGGAGGCCGAGACTCTCTCCTGGAGTCACCCAGGAGAGATGGAGCCATCTCAGTGTGTGGAGGAGCTGGAGGATGATGTGTTCCAAC CAGAGGATGGGGAGCCGGTGACCCAACCCGGGAGCTTGCTCTCTGCTGACCTGTTTGCCCAGAGCCTACTGGACTGCCCCCTCAGCCGACTTCAGCTCTT CCCTCTCACCCACTGCTGTGGCCCTGGCCTTCGACCCACCAGCCAGGAAGACAAAGCTACCCAGACTCTCAGCCCAGCCTCCCCCAGCCAAGGTGTCATG CTGCCTTGTGGGGTGACTGAGGAACCCCAGCGACTCTTTTATGGCAATGCTGGCTATCGGCTTCCTCTCCCTGCCAGTTTCCCAGCAGTCTTGCCCATTG GGGAGCAGCCCCCCGAAGGGCAGTGGCAACATCAAGCAGAGCACCAGCAGAACCAAAATCGTGTGTGGTGGCAGATCCTCCTCTTCCTGCACAACCTTGC TTTGAATGGAGAAGAGAACAGGAACGGGGCAGGCCCTAGGTGAGGGTGGGCTGCCCTCTTCACATGGGGCACCAGGAACACCGTCTGGAACAGGAAGGAC ATCGGGCAGGACTGACACTGTGTCTTGTGAAATTGTTTTTTTGTTGTTATTTTGTGTTTTAATTTTTTTTAATTTCTCTCTGAGTGTACATACAACATAC TCAAGCGGGACCTTCTTTCTCTGTCAGGCCCTTGACCTGGAATGGGGGCCTTTGTCAAACACTGTTGAAGGAGAGGCTGATGTGTCTGTGATGGTGAGAA TTCCCAAGGGCTCTGACAAGTAGATTCTTCGACTGAGGAATCTACCAGTTGTCGAAGATGATCCGTTAGTGATGTTCTCTGGGAAGTGGACTGTGGTTTT TCCAGAGGAACTCAGTTAAGAAATCGAGAGTGGATTAGACTCCCAGTTCCACCAAACCTATGAGCCTTCCACTGTGGATGGGGGCCGTGATCCTGATGGT CACATTGCTTTAACCCAGCAGGGCTTCGGCCAGGGGCTTTCCACTTGAGGATAGCAGCTTCACTAGGCTGGCCGGCCAGCTCCACATCTGACTGGGTTCT TACTTCTCAGCCAGTACCTGCCCCATGGGCTCAAGGATTCCTGGCCAGCTCCTGCCACCTCCAGCAGACCTCAGGGAGGGTTGAGTTTCTCTAAGGACCC CTCAAACATGTCGCAAAATGAACCAAACTTCTGGCTAGGCCTCAAAACTGACTTGGTCCCACTTGGAGGCCCCAGGATTGGTCCTGAGGTACAGAGCCAC TGCCACCACTGGCGGCCTGGGACCAGCTGGGTGTCAGCCACGGATGAGCCGAATAGCCAGTCAGCATGTTGCTGCTGGCAGCCTGTGCCTTTGTCAGCTC TTCTTTCAAAAGACCCCACCGACAGACCGCATTCCACCCCCAACATCGGCACTGAGGGACATCGGGGGCAAGTTTGCAGTGGGGCCGGAAAATATGGTGG CCACCCTACCATGAGAGTCAGCCGTAGGGGACCCCAGACCCCTTGGTTTCCTTGGAAACAACATACCTCTTCCCCCTTATCCCCAGTCCTTTTTCATACC TAGTGGGATACAGAAGAAGCCAGGACAGTGGCTTTGCCAGCTAAGTGACTTTTAGAGTAACAGATAACGATTAGAGTGGGGAACCGTCAAAGCTGGGTAC ACATTTCCTATCTTCCTCCAGCTTCCAGCTAGGCCAGAAGGGCATGCTCTGGAACCCAGCAGGATCACAGCTGCCTGTGCACGTCTTCCCTTCTCTCCCT GCTGCGGCACTTATGGAGAGGATCTAAAGCAGCCGGTGTGGCAGCTCCGATAGCAGGCACAGGGAATCTGTTAACCAGACGCTAGAAACTAAATTATAAC TTTTGCATATGTGAGAAAAGACAACATTGGTGCTAACAGTGAAGTAAGGCCCAAAGGAAAGACGGCTTCCCTGGACAAAGAAGACCTCAGGGCTACCCAA GGAAATAGGAGGAGTCTAGAGTAGACTCACAAATCTGAACAAGCCCAAGTCTTCCAGTTCTGAGGAGAGGAGGTCTTCAGTACTATTGAAGGAGACATTG ATCTTCTGGATGTACAGTTGTGCAGGTTGTTCACTGCACAAGGGCACCTGCAGCTAATGGAGGCTGGAATTCAGCTTGTGTTCTGCTCACTAAGCTGTGT GCGTGCCCTGGTGTGGGGCTACTTCTCCCAAGAAGAAGGGGTGCCTTTCCTAATTTGCAAAGGTGCCATATGGGCTCACAGCACCCTGGAGGAGAAGGGC CTCAAACTGTGCATGTAAGCCTTTGTTTTGTTCTGCTCAGATATTCTGGATATTACCTCTTCTTGGTCAGAATTCTATTCTCAGGGTGTGTACCATGATT TGTCTGCTAAGAAGACGGGTTCCTGCTTTGCAGAGAAGGAGCCGGGGACCAGCTTGAAGACTGGCTCTGGGACACACTGACCATTGTGAAATTCAGCCTT CCCTGTGGGTCTCCATACCATTTTATGATGTGTATGGGACATACGTTGTTCTCCCCTCTCTGGATCAAGGTGGTGACAGGCAGCCTGCTGCCGTATGCTT CAGTGGCACACACCAAAAAACCGGGATTATTTACAGCAGCCACTATTCACCCCTTTTGGCAGACTCTCCACCTGAGTTCAGTGAGAGAGAAAATGATTTA GATCTTGGTGCTGGGGCAAGGCCATCAGCTTCAGAGACCTTGCCAGGCCCAGGCTGGGTGCCCTGTGGCCTGAGAACTGAGCCCAGACTTTGACAAACCC ACCTCAACATCAAGCCTGGGGCAGGTGGAAGGTGGTCTGACTGCACTGTCTCCATCTTATGCAAAGCCAGGAACTCACTCACTGTCTTAAGTGAGCCAGG GCAAAATTTACACCCCTCACGTTTCCCTCTCCCTTCTTCCCCACCAACAAAACCCAGGAGGTAGCTGGCAGAGTAGCTGTCAGGAAGGAGAAAGGTTCTT TCTGGCTGGTTGTTTTTAATTGGCTTAGTGATCTAAACTGGCCCCTTCCTCCTCTGCCTGGTGAGTTGGCCTAAACATTCCTCAAGTCTAGCCTCAGGAG ACCTGCCCCTCCCCCCCGACCTCCACCCCCTCAGACTCCATCCCCCACCCCCAGGAGCCTGGCTGTCTCTAGCGAGGGGTGGAGAATCAGATTTAGAGGG AGGGAGAGTCTGACCTGGATCCTGACCTGTAACCAGCTGAAGACGGTGGAGGCTTTGTGGCTTGCTGAGGGTGGGGGTTGGGAGAGGGACTGGAAACCTT CCTCCTCGGGAAAGAAATGCCTGGGAGGAAGGGAAGCCTGATATTCAGGGTCAAAACAGCCCTTCTAATTCACAACCCCAAAGCAGGGGTTTCTAGAAGT TGGTCAACAGATCTGGTGAGGGAAGTCCCCAGGCCAGACGCTGGACTCCTGCAATGAGGGTGGAGGACTCAGCCTGGCCTCTGCCTGGCCTGTTTCAAAG TCTGGGCCACTGGCAGCCTTCTCTTGCTCAATCCGAGGTGGAAAAAGATCACCTCCCAGTGACCTCCCTCAGCCCCCAGGGGAGTAGGTCTGTGTCCAAA GCTATGTGGCAGGCTTGTTTGAAGGACCCAGAGGGGCCCAGCTGACCTGTCTCACTGCTCCTGGCAGCCCAGCCCCATCGGCAGGTGGCCCCTGCCTGGG GGTTATTCGGGATGGTTCAGCCAGGGCCTCCCAGGAAGAGTGCTGTGGAGCCACGGTGACTGTCCTGGACAGCAAGGAGCATGCTACCCCAGTGAGCACT TCTTTTTGAGGGACTTGATGGGGAGGTGGGGTAGGCAGAAGGGACGTGGCAGAAGCGGGAAGACTTTGGTCACCATGGCTCTGCAGGCCTTCTGCAAATC AGTGCTGGCCTGGGCTGGAAACAGCTCTGTGTGTGAAGGTGAGGACTCTTGGAAGCAGGCCATCCTGGCCAGACACCCTAGCAGGAAGGGGCTCACCTGT CACCCTTAGGCAGCCTGAGGGCTGGTGGGGACTTTTGAGTCTTGAGGGGATAGCGGAAAAAGCTGAGCTCATAGGTGCCCAGCCAGCCTCCCAGCTAAAG GTGCTCAGAGCCCCACTGCCCCCTCTCTCTGTGCAGGTGGCCAGTGCCCCTCCCTGCTCTGGGAGCATTGCTAGCCTTCTACCCCATCCCTGGATCCACA GGGGCTATCGAGGAGACCCAGTGAGAATGTAGCATTTTGTTCATCCCCAGGGTAGCTGCCCTGGGGTCTGGGCACTCTGCCTCTGGGAGAGAGGAAGAAG AAAGGGGCCCCATTTTTTAAAAAACTGTACAGAGCCTTTGGCTTTATGTGTTTATGTTCTTCACATGCATATGTGTGTATGTGTGTATATCTTTCCCCCC ATCAATTGGTACAATTTTTAATAAAATCATTTAAAGCAAA

OL001006 (forward primer)
bmf.r.4 (reverse primer)
Single exon skipping

Frame 0

GVALRTGAREGFGLRTSGVTMKNWGAGVRERGA EPTVGCAAPRGNLGVAFGTRRSWVQVGRGTWGAL LSRPGRPRTAQPLGLTLG*DRSCGASCLSAESE GSSP*QLGFFPPSQSSLGVQPPSARHAGPWRGA LASRLGGRDSLLESPRRDGAISVCGGAGG*CVPT RGWGAGDPTRELALC*PVCPEPTGLPPQPTSAL PSHPLLWPWPSTHQPGRQSYPDSQPSLPQPRCH AALWGD*GTPATLLWQCWLSASSPCQFPSSLAHW GAAPRRAVATSSRAPAEPKSCVVADPPLPAQPC FEWRREQERGRP*VRVGCPLHMGHQEHRLEQEG HRAGLTLCLVKLFFCCYFVF*FFLISL*VYIQHT QAGPSFSVRPLTWNGGLCQTLLKERLMCL*W*E FPRALTSRFFD*GIYQLSKMIR**CSLGSGLWF FQRNSVKKSRVD*TPSSTKPMSLPLWMGAVILMV TLL*PSRASARGFPLEDSSFTRLAGQLHI*LGS YFSASTCPMGSRIPGQLLPPPADLREG*VSLRT PQTCRKMNQTSG*ASKLTWSHLEAPGLVLRYRAT ATTGGLGPAGCQPRMSRIASQHVAAGSLCLCQL FFQKTPPTDRIPPPTSALRDIGGKFAVGPENMV ATLP*ESAVGDPRPLGFLGNNIPLPPYPQSFFIP SGIQKKPGQWLCQLSDF*SNR*RLEWGTVKAGY TFPIFLQLPARPEGHALEPSRITAACARLPFSP CCGTYGEDLKQPVWQLR*QAQGIC*PDARN*IIT FAYVRKDNIGANSEVRPKGKTASLDKEDLRATQ GNRRSLE*THKSEQAQVFQF*GEEVFSTIEGDI DLLDVQLCRLFTAQGHLQLMEAGIQLVFCSLSCV RALVWGYFSQEEGVPFLICKGAIWAHSTLEEKG LKLCM*AFVLFCSDILDITSSWSEFYSQGVYHD LSAKKTGSCFAEKEPGTSLKTGSGTH*PL*NSAF PVGLHTIL*CVWDIRCSPLSGSRW*QAACCRML QWHTPKNRDYLQQPLFTPFGRLST*VQ*ERK*F RSWCWGKAISFRDLARPRLGALWPEN*AQTLTNP PQHQAWGRWKVV*LHCLHLMQSQELTHCLK*AR AKFTPLTFPSPFFPTNKTQEVAGRVAVRKEKGS FWLVVFNWLSDLNWPLPPLPGELA*TFLKSSLRR PAPPPRPPPPQTPSPTPRSLAVSSEGWRIRFRG RESLTWILTCNQLKTVEALWLAEGGGWERDWKP SSSGKKCLGGREA*YSGSKQPF*FTTPKQGFLEV GQQIW*GKSPGQTLDSCNEGGGLSLASAWPVSK SGPLAAFSCSIRGGKRSPPSDLPQPPGE*VCVQ SYVAGLFEGPRGAQLTCLTAPGSPAPSAGGPCLG VIRDGSARASQEECCGATVTVLDSKEHATPVST SF*GT*WGGGVGRRDVAEAGRLWSPWLCRPSAN QCWPGLETALCVKVRTLGSRPSWPDTLAGRGSPV TLRQPEGWWGLLSLEGIAEKAELIGAQPASQLK VLRAPLPPLSVQVASAPPCSGSIASLLPHPWIH RGYRGDPVRM*HFVHPQGSCPGVWALCLWERGRR KGPHFLKNCTEPLALCVYVLHMHMCVCVYIFPP INWYNF**NHLKQ

Frame 1

ESRCARGPGKGLGCGRLG*P*RTGALG*GNGVQ NPQLGALLQEGTWG*HLGLEGVGSR*AGEPGVH C*ADLGARERPSLWV*LWDRTGAVGHPASLRSLK APLLNSWAFSLLPNRVWASSPRVLVTLDPGAEP WHHDSEAETLSWSHPGEMEPSQCVEELEDDVFQ PEDGEPVTQPGSLLSADLFAQSLLDCPLSRLQLF PLTHCCGPGLRPTSQEDKATQTLSPASPSQGVM LPCGVTEEPQRLFYGNAGYRLPLPASFPAVLPI GEQPPEGQWQHQAEHQQNQNRVWWQILLFLHNLA LNGEENRNGAGPR*GWAALFTWGTRNTVWNRKD IGQD*HCVL*NCFFVVILCFNFF*FLSECTYNI LKRDLLSLSGP*PGMGAFVKHC*RRG*CVCDGEN SQGL*QVDSSTEESTSCRR*SVSDVLWEVDCGF SRGTQLRNREWIRLPVPPNL*AFHCGWGP*S*W SHCFNPAGLRPGAFHLRIAASLGWPASSTSDWVL TSQPVPAPWAQGFLASSCHLQQTSGRVEFL*GP LKHVAK*TKLLARPQN*LGPTWRPQDWS*GTEP LPPLAAWDQLGVSHG*AE*PVSMLLLAACAFVSS SFKRPHRQTAFHPQHRH*GTSGASLQWGRKIWW PPYHESQP*GTPDPLVSLETTYLFPLIPSPFSY LVGYRRSQDSGFAS*VTFRVTDND*SGEPSKLGT HFLSSSSFQLGQKGMLWNPAGSQLPVHVFPSLP AAALMERI*SSRCGSSDSRHRESVNQTLETKL* LLHM*EKTTLVLTVK*GPKERRLPWTKKTSGLPK EIGGV*SRLTNLNKPKSSSSEERRSSVLLKETL IFWMYSCAGCSLHKGTCS*WRLEFSLCSAH*AV CVPWCGATSPKKKGCLS*FAKVPYGLTAPWRRRA SNCACKPLFCSAQIFWILPLLGQNSILRVCTMI CLLRRRVPALQRRSRGPA*RLALGHTDHCEIQP SLWVSIPFYDVYGTYVVLPSLDQGGDRQPAAVCF SGTHQKTGIIYSSHYSPLLADSPPEFSERENDL DLGAGARPSASETLPGPGWVPCGLRTEPRL*QT HLNIKPGAGGRWSDCTVSILCKARNSLTVLSEPG QNLHPSRFPLPSSPPTKPRR*LAE*LSGRRKVL SGWLFLIGLVI*TGPFLLCLVSWPKHSSSLASG DLPLPPDLHPLRLHPPPPGAWLSLARGGESDLEG GRV*PGS*PVTS*RRWRLCGLLRVGVGRGTGNL PPRERNAWEEGKPDIQGQNSPSNSQPQSRGF*K LVNRSGEGSPQARRWTPAMRVEDSAWPLPGLFQS LGHWQPSLAQSEVEKDHLPVTSLSPQGSRSVSK AMWQACLKDPEGPS*PVSLLLAAQPHRQVAPAW GLFGMVQPGPPRKSAVEPR*LSWTARSMLPQ*AL LFEGLDGEVG*AEGTWQKREDFGHHGSAGLLQI SAGLGWKQLCV*R*GLLEAGHPGQTP*QEGAHL SPLGSLRAGGDF*VLRG*RKKLSS*VPSQPPS*R CSEPHCPLSLCRWPVPLPALGALLAFYPIPGST GAIEETQ*ECSILFIPRVAALGSGHSASGREEE ERGPIF*KTVQSLWLYVFMFFTCICVYVCISFPP SIGTIFNKII*SK

Frame 2

SRAAHGGPGRVWAADVWGDHEELGRWGEGTGCR THSWVRCSKRELGGSIWDSKELGPGRQGNLGCT AEQTWAPENGPASGSDFGIGQELWGILPLCGV* RLLSLTAGLFPSFPIESGRPAPECSSRWTLARSP GITTRRPRLSPGVTQERWSHLSVWRSWRMMCSN QRMGSR*PNPGACSLLTCLPRAYWTAPSADFSS SLSPTAVALAFDPPARKTKLPRLSAQPPPAKVSC CLVG*LRNPSDSFMAMLAIGFLSLPVSQQSCPL GSSPPKGSGNIKQSTSRTKIVCGGRSSSSCTTL L*MEKRTGTGQALGEGGLPSSHGAPGTPSGTGRT SGRTDTVSCEIVFLLLFCVLIFFNFSLSVHTTY SSGTFFLCQALDLEWGPLSNTVEGEADVSVMVR IPKGSDK*ILRLRNLPVVEDDPLVMFSGKWTVVF PEELS*EIESGLDSQFHQTYEPSTVDGGRDPDG HIALTQQGFGQGLST*G*QLH*AGRPAPHLTGF LLLSQYLPHGLKDSWPAPATSSRPQGGLSFSKDP SNMSQNEPNFWLGLKTDLVPLGGPRIGPEVQSH CHHWRPGTSWVSATDEPNSQSACCCWQPVPLSA LLSKDPTDRPHSTPNIGTEGHRGQVCSGAGKYGG HPTMRVSRRGPQTPWFPWKQHTSSPLSPVLFHT *WDTEEARTVALPAK*LLE*QITIRVGNRQSWV HISYLPPASS*ARRACSGTQQDHSCLCTSSLLSL LRHLWRGSKAAGVAAPIAGTGNLLTRR*KLNYN FCICEKRQHWC*Q*SKAQRKDGFPGQRRPQGYP RK*EESRVDSQI*TSPSLPVLRRGGLQYY*RRH* SSGCTVVQVVHCTRAPAANGGWNSACVLLTKLC ACPGVGLLLPRRRGAFPNLQRCHMGSQHPGGEG PQTVHVSLCFVLLRYSGYYLFLVRILFSGCVP*F VC*EDGFLLCREGAGDQLEDWLWDTLTIVKFSL PCGSPYHFMMCMGHTLFSPLWIKVVTGSLLPYA SVAHTKKPGLFTAATIHPFWQTLHLSSVREKMI* ILVLGQGHQLQRPCQAQAGCPVA*ELSPDFDKP TSTSSLGQVEGGLTALSPSYAKPGTHSLS*VSQ GKIYTPHVSLSLLPHQQNPGGSWQSSCQEGERFF LAGCF*LA**SKLAPSSSAW*VGLNIPQV*PQE TCPSPPTSTPSDSIPHPQEPGCL*RGVENQI*R EGESDLDPDL*PAEDGGGFVAC*GWGLGEGLETF LLGKEMPGRKGSLIFRVKTALLIHNPKAGVSRS WSTDLVREVPRPDAGLLQ*GWRTQPGLCLACFK VWATGSLLLLNPRWKKITSQ*PPSAPRGVGLCPK LCGRLV*RTQRGPADLSHCSWQPSPIGRWPLPG GYSGWFSQGLPGRVLWSHGDCPGQQGACYPSEH FFLRDLMGRWGRQKGRGRSGKTLVTMALQAFCKS VLAWAGNSSVCEGEDSWKQAILARHPSRKGLTC HP*AA*GLVGTFES*GDSGKS*AHRCPASLPAK GAQSPTAPSLCAGGQCPSLLWEHC*PSTPSLDPQ GLSRRPSENVAFCSSPG*LPWGLGTLPLGERKK KGAPFFKKLYRAFGFMCLCSSHAYVCMCVYLSP HQLVQFLIKSFKA