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Sequences

Sequence SIVA.eAug05

SIVA_HUMAN-3 (forward primer)
SIVA_HUMAN-4 (reverse primer)
Intron retention

Frame 0

CDVTASLVRGWRPGSCVAPGPAAMPKRSCPFAD VAPLQLKVRVSQRELSRGVCAERYSQEVFEKTKR LLFLGAQAYLDHVWDEGCAVVHLPESPKPGPTG APRAARGQMLIGPDGRLIRSLGQASEADPSGVA SIACSSCVRAVDGKAVCGQCERALCGQCVRTCWG CGSVACTLCGLVDDMYEKVLCTSCAMFET*GWL KPAAFTGSHAVHGSLPWTSARCSH*TVGSTGGV PFTCSILYPNDRMNKPLYICTRG

Frame 1

VTSRRRW*GAGGRGAA*LPAPRPCPSGAAPSRT WPRYSSRSA*ARGS*AAACAPSATRRRSSRRPS DSCSSGPRPTWTTCGMKAVPSFTCQSPQSLALQG PRGLHVGRC*LDQTAA*SGALGRPPKLTHLG*R PLPVPHACEPWMGRRSAVSVSEPCAGSVCAPAG AAAPWPVPCVASWMTCTRKCCAPAVPCSRPEAGS SRLPSPGATPCMAAFPGRALGVHTELWGRREGC LLHVLFCILMTE*INLFIFAQED

Frame 2

*RHGVVGKGLAAGELRSSRPRGHAQAELPLRGR GPATAQGPREPEGVEPRRVRRALLAGGLREDQA TPVPRGPGLPGPRVG*RLCRRSPARVPKAWPYR GPEGCTWADADWTRRPPDQEPWAGLRS*PIWGSV HCLFLMRASRGWEGGLRSV*ASPVRAVCAHLLG LRLRGLYPVWPRG*HVRESAVHQLCHVRDLRLA QAGCLHREPRRAWQPSLDERSVFTLNCGVDGRGA FYMFYFVS**QNE*TSLYLHKR

Sequence SIVA.fAug05

TGTGACGTCACGGCGTCGTTGGTAAGGGGCTGGCGGCCGGGGAGCTGCGTAGCTCCCGGCCCCGCGGCCATGCCCAAGCGGAGCTGCCCCTTCGCGGACG TGGCCCCGCTACAGCTCAAGGTCCGCGTGAGCCAGAGGGAGTTGAGCCGCGGCGTGTGCGCCGAGCGCTACTCGCAGGAGGTCTTCGAGAAGACCAAGCG ACTCCTGTTCCTCGGGGCCCAGGCCTACCTGGACCACGTGTGGGATGAAGGCTGTGCCGTCGTTCACCTGCCAGAGTCCCCAAAGCCTGGCCCTACAGGG GCCCCGAGGGCTGCACGTGGGCAGATGCTGATTGGACCAGACGGCCGCCTGATCAGGAGCCTTGGGCAGGCCTCCGAAGCTGACCCATCTGGGGTAGCGT CCATTGCCTGTTCCTCATGCGTGCGAGCCGTGGATGGGAAGGCGGTCTGCGGTCAGTGTGAGCGAGCCCTGTGCGGGCAGTGTGTGCGCACCTGCTGGGG CTGCGGCTCCGTGGCCTGTACCCTGTGTGGCCTCGTGGAGTAAGTACTTCAGTCCCTGGAGCTGCTGAGATCCCATAGCCCCAGCAAGCCGTGATGGGGG ACGGGTGGGTCACCCATGTGGGCCCAGAACACACACGTGTGGCCCCTGATGCAGTGCCATCTGGCATTGCCTAACGGGACATGGTGGCAATAGATGCTTG GCCCAACTTTAGTGGTTAGTAATCCTCTCTAAGGGAAAGCTGAACCTCACAGATGACCTGCTCTGTATCCGGCTCTAGTCCCTGTCTTCAGCCACTTCCT GTTCTGATCTTTGAGCACCCCCACCTCCTCCTCCTCTGGTCTTTGAGCCCCCCCTCCCCCCGTCCGAGGAGCCCCTGGTTGGTGACACAGGTGCACACCG CAGCTTCACCACAGTCATGCGTGCCGTTTGGTTGTAGACCCTCTCAGGCAGGAAGTGGAGTGTTTGTGTAGTCTCCCATTTGTGGGTGGGTTTGCTGCAG CTGGCTGGATCCCGTCCTTACTCCTAAGGTGGGGAGGGGCGTTCTCACCCTAGGGGCTACTCACCTGGTGGATGGGAGTGGACAGTGTGGAGCCTGTTTG CCCTGCCCCGTGAGAAAGATGACTTCATCCAGACACATGTGGAACTGGCTCCATAGACCCAAAGCAAGCTTAGTCCAAACAATGTCTGAAATTGTCCATC TAAAATAGAAACCACATGTTACATCTCGAGTCCTTTCTCTCGAGCCTTTCTTCAGTCGGGGCAGAGGCCCAGACTCATTGGAGACGGAGAGCCCTGGGCA GGGGGGGCAGCACCAGGGAGAAGCGCCGCGTGCCAGTGATCGGCTCCCATGGCTTCAGCATGGGCAGGAGTGGGGTAACGCACCCCATCCAGGTTGGGAA TCATCTGCAGGGCTAGCTATGAATGTGGGATACTCTGTCTTTGCTTTTCACCCGCTGAGTTCATCTTCTTTCCTAGAAGTAAACTGAAAAGGGCAGGGTT TTCTCATTGTCTGGTGGCCCTCTCACCAAAGCAGAAGTGAACATAGCCATGGGCAGGTTCAGCGGAGAGGTGGGTCCCAGAGCGCTGCGTGGCTGGCCTC CCACTCCTGCCCTGCACACCATCCTTGAGGGACTCCCTTGCGCCCATTTCCTCCTGGCCTCTACCTACTTTCTGCTGCTGCTTCCTCCCATACCCACAGA GTTCACGTTGAGCTGAGTGGGCCACTGTCCCCACTGTGGACACACTCTTTTATCGCAGCTACAGCACAGGAGGTGGGCTCTGCCCTCTTTCACAAATAAA TGACTTGTTCCAGGTCACAAGCCAGAAGTGGCATTCCCAGGCCAGGCAGCCCAGCTGGTGGCACCCGCAGTCTCCTACCCCCTCCCCTGCCATTTCCTAC TTCAGGACCAGGCGTCGCAGGAGTGGGGGAAAACAGGGATCTGTCCACACGGCTCTGGTTTAACACAGATGCAGCCCAGCAGCCTTTGGCGTCCTGCTGA TGGCCTGCACTGGCCCACGCCTGGCCCGTCCTCTGCAGGGCTCTCCAGCTGGCTGCCGGTGGCGGTGAGGGGCTGTCTTCGCGGCTCACCCACAATACTA CTGGCAAACTCTGCAAACCAAGTGGCAAGCAGTTCTGGCTGCCTTGTTGACTCCACGGCCCTGTCGTGTTTGGGGTAGCCATTGCCAGTCCCGTTTCCTC GCAACATCTCTCTTAATCACTTTCTCCCTGTGTTTTGCCGGGTTCCCTAGCCTTTACCTTTAGAGCTTTCTTTTTTTTTTTTTTTTGAGACGGAGTCTCG CTCTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACTTCCACCTCCTGTGTTCAAGCAATTTTCCTGTCTCAGCCTCCCAAGTAGC TGGAGTTACAGGCGTGCGCTAGCACGCCCAGCTAATTTTTGTATTCTTAGTAGAGACAGAGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACC TCAGGTGATCCTCCGGCCTCAGCCTCCCAAAGTGCTGAGGTAAGCCACACCATACGCGTGAGCCACCATGCCCGGCCCTACCCTTAGTCTTGATATATCA GAAAAGCACTGTTTGATGTGCTTCAGTGTAAACCATTGTGGTTCGGTGAATTCTGGCAGGTCATCTGGAGGTTTCTTTGGAGAGCTTCTATCCTACTGGA AACCCAGGTTGGGCCCCATGTGGATTGATCGGCAGTGGGAATCAGCTAGGGCTGCTGTAACTGAGTTCGCAGACCCAGTGGCATAGACAGCAAAGAGGTA CTGCCACGTGGCTTTGGAGACCAGAAGTCTGAGAGGAAGGTGCTGGCAGGGCTGGTTCCTTCTGAGGCTGCAATGGAAAACCTGTCCTGGCCTCTCTCCT GGCATCTGCTGGTTATCTTTGGTGTTCCCTGTAGACAGCTGCCCCCTCCCTGTATCTTCATGTCGTCTTCCTTCTCTGTGTCCTTCTCTTCACGTAGTCT TTTTAGGACGCTGGTCGTGTTGCCTTAGGGCCCCCATGTCAGTGCCGTCATCTAGACTGATGATGCCCGCAGTGATCCTGTCTCCAGATAAGGTCATGTC CTGAGGTGTTCTGGGTTAGGACTTTGACGTTTGAATGGTGGGGGGTGGTGGACACAATTCAGCCCCTGACAGCAGCTTTTCTCTCCCCTCCCTGACGCTG TCGCAGCTGCAGTGACATGTACGAGAAAGTGCTGTGCACCAGCTGTGCCATGTTCGAGACCTGAGGCTGGCTCAAGCCGGCTGCCTTCACCGGGAGCCAC GCCGTGCATGGCAGCCTTCCCTGGACGAGCGCTCGGTGTTCACACTGAACTGTGGGGTCGACGGGAGGGGTGCCTTTTACATGTTCTATTTTGTATCCTA ATGACAGAATGAATAAACCTCTTTATATTTGCACAA

SIVA_HUMAN-3 (forward primer)
SIVA_HUMAN-4 (reverse primer)
Intron retention

Frame 0

CDVTASLVRGWRPGSCVAPGPAAMPKRSCPFAD VAPLQLKVRVSQRELSRGVCAERYSQEVFEKTKR LLFLGAQAYLDHVWDEGCAVVHLPESPKPGPTG APRAARGQMLIGPDGRLIRSLGQASEADPSGVA SIACSSCVRAVDGKAVCGQCERALCGQCVRTCWG CGSVACTLCGLVE*VLQSLELLRSHSPSKP*WG TGGSPMWAQNTHVWPLMQCHLALPNGTWWQ*ML GPTLVVSNPL*GKAEPHR*PALYPALVPVFSHFL F*SLSTPTSSSSGL*APPPPVRGAPGW*HRCTP QLHHSHACRLVVDPLRQEVECLCSLPFVGGFAA AGWIPSLLLRWGGAFSP*GLLTWWMGVDSVEPVC PAP*ER*LHPDTCGTGSIDPKQA*SKQCLKLSI *NRNHMLHLESFLSSLSSVGAEAQTHWRRRALG RGGSTREKRRVPVIGSHGFSMGRSGVTHPIQVGN HLQG*L*MWDTLSLLFTR*VHLLS*K*TEKGRV FSLSGGPLTKAEVNIAMGRFSGEVGPRALRGWP PTPALHTILEGLPCAHFLLASTYFLLLLPPIPTE FTLS*VGHCPHCGHTLLSQLQHRRWALPSFTNK *LVPGHKPEVAFPGQAAQLVAPAVSYPLPCHFL LQDQASQEWGKTGICPHGSGLTQMQPSSLWRPAD GLHWPTPGPSSAGLSSWLPVAVRGCLRGSPTIL LANSANQVASSSGCLVDSTALSCLG*PLPVPFP RNISLNHFLPVFCRVP*PLPLELSFFFFFETESR SVTQAGVQWCDLGSLQLPPPVFKQFSCLSLPSS WSYRRALARPANFCILSRDRVSPCWPGWSRTPD LR*SSGLSLPKC*GKPHHTREPPCPALPLVLIYQ KSTV*CASV*TIVVR*ILAGHLEVSLESFYPTG NPGWAPCGLIGSGNQLGLL*LSSQTQWHRQQRG TATWLWRPEV*EEGAGRAGSF*GCNGKPVLASLL ASAGYLWCSL*TAAPSLYLHVVFLLCVLLFT*S F*DAGRVALGPPCQCRHLD**CPQ*SCLQIRSC PEVFWVRTLTFEWWGVVDTIQPLTAAFLSPP*RC RSCSDMYEKVLCTSCAMFET*GWLKPAAFTGSH AVHGSLPWTSARCSH*TVGSTGGVPFTCSILYP NDRMNKPLYICT

Frame 1

VTSRRRW*GAGGRGAA*LPAPRPCPSGAAPSRT WPRYSSRSA*ARGS*AAACAPSATRRRSSRRPS DSCSSGPRPTWTTCGMKAVPSFTCQSPQSLALQG PRGLHVGRC*LDQTAA*SGALGRPPKLTHLG*R PLPVPHACEPWMGRRSAVSVSEPCAGSVCAPAG AAAPWPVPCVASWSKYFSPWSC*DPIAPASRDGG RVGHPCGPRTHTCGP*CSAIWHCLTGHGGNRCL AQL*WLVILSKGKLNLTDDLLCIRL*SLSSATS CSDL*APPPPPPLVFEPPLPPSEEPLVGDTGAHR SFTTVMRAVWL*TLSGRKWSVCVVSHLWVGLLQ LAGSRPYS*GGEGRSHPRGYSPGGWEWTVWSLF ALPREKDDFIQTHVELAP*TQSKLSPNNV*NCPS KIETTCYISSPFSRAFLQSGQRPRLIGDGEPWA GGAAPGRSAACQ*SAPMASAWAGVG*RTPSRLG IICRASYECGILCLCFSPAEFIFFPRSKLKRAGF SHCLVALSPKQK*T*PWAGSAERWVPERCVAGL PLLPCTPSLRDSLAPISSWPLPTFCCCFLPYPQ SSR*AEWATVPTVDTLFYRSYSTGGGLCPLSQIN DLFQVTSQKWHSQARQPSWWHPQSPTPSPAISY FRTRRRRSGGKQGSVHTALV*HRCSPAAFGVLL MACTGPRLARPLQGSPAGCRWR*GAVFAAHPQYY WQTLQTKWQAVLAALLTPRPCRVWGSHCQSRFL ATSLLITFSLCFAGFPSLYL*SFLFFFFLRRSL ALSPRLECSGAILAHCNFHLLCSSNFPVSASQVA GVTGVR*HAQLIFVFLVETEFHHVGQAGLELLT SGDPPASASQSAEVSHTIRVSHHARPYP*S*YI RKALFDVLQCKPLWFGEFWQVIWRFLWRASILLE TQVGPHVD*SAVGIS*GCCN*VRRPSGIDSKEV LPRGFGDQKSERKVLAGLVPSEAAMENLSWPLS WHLLVIFGVPCRQLPPPCIFMSSSFSVSFSSRSL FRTLVVLP*GPHVSAVI*TDDARSDPVSR*GHV LRCSGLGL*RLNGGGWWTQFSP*QQLFSPLPDA VAAAVTCTRKCCAPAVPCSRPEAGSSRLPSPGAT PCMAAFPGRALGVHTELWGRREGCLLHVLFCIL MTE*INLFIFAQ

Frame 2

*RHGVVGKGLAAGELRSSRPRGHAQAELPLRGR GPATAQGPREPEGVEPRRVRRALLAGGLREDQA TPVPRGPGLPGPRVG*RLCRRSPARVPKAWPYR GPEGCTWADADWTRRPPDQEPWAGLRS*PIWGSV HCLFLMRASRGWEGGLRSV*ASPVRAVCAHLLG LRLRGLYPVWPRGVSTSVPGAAEIP*PQQAVMG DGWVTHVGPEHTRVAPDAVPSGIA*RDMVAIDAW PNFSG**SSLRES*TSQMTCSVSGSSPCLQPLP VLIFEHPHLLLLWSLSPPSPRPRSPWLVTQVHT AASPQSCVPFGCRPSQAGSGVFV*SPICGWVCCS WLDPVLTPKVGRGVLTLGATHLVDGSGQCGACL PCPVRKMTSSRHMWNWLHRPKASLVQTMSEIVH LK*KPHVTSRVLSLEPFFSRGRGPDSLETESPGQ GGQHQGEAPRASDRLPWLQHGQEWGNAPHPGWE SSAGLAMNVGYSVFAFHPLSSSSFLEVN*KGQG FLIVWWPSHQSRSEHSHGQVQRRGGSQSAAWLAS HSCPAHHP*GTPLRPFPPGLYLLSAAASSHTHR VHVELSGPLSPLWTHSFIAATAQEVGSALFHK* MTCSRSQARSGIPRPGSPAGGTRSLLPPPLPFPT SGPGVAGVGENRDLSTRLWFNTDAAQQPLASC* WPALAHAWPVLCRALQLAAGGGEGLSSRLTHNT TGKLCKPSGKQFWLPC*LHGPVVFGVAIASPVSS QHLS*SLSPCVLPGSLAFTFRAFFFFFF*DGVS LCHPGWSAVVRSWLTATSTSCVQAIFLSQPPK* LELQACASTPS*FLYS**RQSFTMLARLVSNS*P QVILRPQPPKVLR*ATPYA*ATMPGPTLSLDIS EKHCLMCFSVNHCGSVNSGRSSGGFFGELLSYW KPRLGPMWIDRQWESARAAVTEFADPVA*TAKRY CHVALETRSLRGRCWQGWFLLRLQWKTCPGLSP GICWLSLVFPVDSCPLPVSSCRLPSLCPSLHVV FLGRWSCCLRAPMSVPSSRLMMPAVILSPDKVMS *GVLG*DFDV*MVGGGGHNSAPDSSFSLPSLTL SQLQ*HVRESAVHQLCHVRDLRLAQAGCLHREP RRAWQPSLDERSVFTLNCGVDGRGAFYMFYFVS* *QNE*TSLYLH

Sequence SIVA.dAug05

TGTGACGTCACGGCGTCGTTGGTAAGGGGCTGGCGGCCGGGGAGCTGCGTAGCTCCCGGCCCCGCGGCCATGCCCAAGCGGAGCTGCCCCTTCGCGGACG TGGCCCCGCTACAGCTCAAGGTCCGCGTGAGCCAGAGGGAGTTGAGCCGCGGCGTGTGCGCCGAGCGCTACTCGCAGGAGGTCTTCGAGAAGACCAAGCG ACTCCTGTTCCTCGGGGCCCAGGCCTACCTGGACCACGTGTGGGATGAAGGCTGTGCCGTCGTTCACCTGCCAGAGTCCCCAAAGCCTGGCCCTACAGGG GCCCCGAGGGCTGCACGTGGGCAGATGCTGATTGGACCAGACGGCCGCCTGATCAGGAGCCTTGGGCAGGCCTCCGAAGCTGGTGAGTGGCACCAGCAGC CCTTTGTGGCTGTGGCACTGAGGGCAGGTGGTGGCACGAGCATTTTTCTTGATTTGCAAGGTCAGGCTTTTCCTCCCTGGGTAAGCAGGACTCTGACCCA GAGCTTGGCCTTCTGGCTCAGAAGGCTCCTGTTATCAGGAGGTCTCACATTCAAGACTGGAAGTTGTTAAACAGCTTCTAAGTACTGGGCCAAAAAAGCA GCAGAGACTCCCCGGGTATCCCGTCTGCTTTCCGCCTGCAGCCCCCTCAGATAGTCCCACAAGGCTGGAAACATGCCAGTCCCATCCCTATGCAGTGTGG GGTCTCAGAGCATCACCTGTGTTGCTGGTTAGAAATGCAATCCCCGACTCTACCTCAAGTCTGGTTGGTGTGTCTAGGAGGAGCCCAGGCACCTCTTTGT TAACCAGTAAGGCAGTGGGCCACACTGAGACCCAGATGGGACACATGGAGTTCTGACAAGCAACAGGGGAAGAATGGTCCTTCCAGCACTAGCCTCCAGG TAGCAGAGGGACCTGGTAAGGGAAAGAGCACTGAACTTGGAGTCAACTACAGGCATATCTCACCTTGGATCTCTGTAGTAAAACGGGGGTCCTGTGCACT GTACCAGGCTCACGGGTTGTCAGAAGGATCCAGTGTAGGTGGGGATGGGGTAGGCAGGTCAGAGCCCTCTGCCCTCAGGTATGCATGGAGGCAGGTAGCC CGGCAGTCCCGGAAACTCCAGTCTCCTTGCAGCCTGACGGCTTGGCGTTTCTTCCTCACAGACCCATCTGGGGTAGCGTCCATTGCCTGTTCCTCATGCG TGCGAGCCGTGGATGGGAAGGCGGTCTGCGGTCAGTGTGAGCGAGCCCTGTGCGGGCAGTGTGTGCGCACCTGCTGGGGCTGCGGCTCCGTGGCCTGTAC CCTGTGTGGCCTCGTGGAGTAAGTACTTCAGTCCCTGGAGCTGCTGAGATCCCATAGCCCCAGCAAGCCGTGATGGGGGACGGGTGGGTCACCCATGTGG GCCCAGAACACACACGTGTGGCCCCTGATGCAGTGCCATCTGGCATTGCCTAACGGGACATGGTGGCAATAGATGCTTGGCCCAACTTTAGTGGTTAGTA ATCCTCTCTAAGGGAAAGCTGAACCTCACAGATGACCTGCTCTGTATCCGGCTCTAGTCCCTGTCTTCAGCCACTTCCTGTTCTGATCTTTGAGCACCCC CACCTCCTCCTCCTCTGGTCTTTGAGCCCCCCCTCCCCCCGTCCGAGGAGCCCCTGGTTGGTGACACAGGTGCACACCGCAGCTTCACCACAGTCATGCG TGCCGTTTGGTTGTAGACCCTCTCAGGCAGGAAGTGGAGTGTTTGTGTAGTCTCCCATTTGTGGGTGGGTTTGCTGCAGCTGGCTGGATCCCGTCCTTAC TCCTAAGGTGGGGAGGGGCGTTCTCACCCTAGGGGCTACTCACCTGGTGGATGGGAGTGGACAGTGTGGAGCCTGTTTGCCCTGCCCCGTGAGAAAGATG ACTTCATCCAGACACATGTGGAACTGGCTCCATAGACCCAAAGCAAGCTTAGTCCAAACAATGTCTGAAATTGTCCATCTAAAATAGAAACCACATGTTA CATCTCGAGTCCTTTCTCTCGAGCCTTTCTTCAGTCGGGGCAGAGGCCCAGACTCATTGGAGACGGAGAGCCCTGGGCAGGGGGGGCAGCACCAGGGAGA AGCGCCGCGTGCCAGTGATCGGCTCCCATGGCTTCAGCATGGGCAGGAGTGGGGTAACGCACCCCATCCAGGTTGGGAATCATCTGCAGGGCTAGCTATG AATGTGGGATACTCTGTCTTTGCTTTTCACCCGCTGAGTTCATCTTCTTTCCTAGAAGTAAACTGAAAAGGGCAGGGTTTTCTCATTGTCTGGTGGCCCT CTCACCAAAGCAGAAGTGAACATAGCCATGGGCAGGTTCAGCGGAGAGGTGGGTCCCAGAGCGCTGCGTGGCTGGCCTCCCACTCCTGCCCTGCACACCA TCCTTGAGGGACTCCCTTGCGCCCATTTCCTCCTGGCCTCTACCTACTTTCTGCTGCTGCTTCCTCCCATACCCACAGAGTTCACGTTGAGCTGAGTGGG CCACTGTCCCCACTGTGGACACACTCTTTTATCGCAGCTACAGCACAGGAGGTGGGCTCTGCCCTCTTTCACAAATAAATGACTTGTTCCAGGTCACAAG CCAGAAGTGGCATTCCCAGGCCAGGCAGCCCAGCTGGTGGCACCCGCAGTCTCCTACCCCCTCCCCTGCCATTTCCTACTTCAGGACCAGGCGTCGCAGG AGTGGGGGAAAACAGGGATCTGTCCACACGGCTCTGGTTTAACACAGATGCAGCCCAGCAGCCTTTGGCGTCCTGCTGATGGCCTGCACTGGCCCACGCC TGGCCCGTCCTCTGCAGGGCTCTCCAGCTGGCTGCCGGTGGCGGTGAGGGGCTGTCTTCGCGGCTCACCCACAATACTACTGGCAAACTCTGCAAACCAA GTGGCAAGCAGTTCTGGCTGCCTTGTTGACTCCACGGCCCTGTCGTGTTTGGGGTAGCCATTGCCAGTCCCGTTTCCTCGCAACATCTCTCTTAATCACT TTCTCCCTGTGTTTTGCCGGGTTCCCTAGCCTTTACCTTTAGAGCTTTCTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGT GCAGTGGTGCGATCTTGGCTCACTGCAACTTCCACCTCCTGTGTTCAAGCAATTTTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTTACAGGCGTGCGCTA GCACGCCCAGCTAATTTTTGTATTCTTAGTAGAGACAGAGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCTCCGGCCTCA GCCTCCCAAAGTGCTGAGGTAAGCCACACCATACGCGTGAGCCACCATGCCCGGCCCTACCCTTAGTCTTGATATATCAGAAAAGCACTGTTTGATGTGC TTCAGTGTAAACCATTGTGGTTCGGTGAATTCTGGCAGGTCATCTGGAGGTTTCTTTGGAGAGCTTCTATCCTACTGGAAACCCAGGTTGGGCCCCATGT GGATTGATCGGCAGTGGGAATCAGCTAGGGCTGCTGTAACTGAGTTCGCAGACCCAGTGGCATAGACAGCAAAGAGGTACTGCCACGTGGCTTTGGAGAC CAGAAGTCTGAGAGGAAGGTGCTGGCAGGGCTGGTTCCTTCTGAGGCTGCAATGGAAAACCTGTCCTGGCCTCTCTCCTGGCATCTGCTGGTTATCTTTG GTGTTCCCTGTAGACAGCTGCCCCCTCCCTGTATCTTCATGTCGTCTTCCTTCTCTGTGTCCTTCTCTTCACGTAGTCTTTTTAGGACGCTGGTCGTGTT GCCTTAGGGCCCCCATGTCAGTGCCGTCATCTAGACTGATGATGCCCGCAGTGATCCTGTCTCCAGATAAGGTCATGTCCTGAGGTGTTCTGGGTTAGGA CTTTGACGTTTGAATGGTGGGGGGTGGTGGACACAATTCAGCCCCTGACAGCAGCTTTTCTCTCCCCTCCCTGACGCTGTCGCAGCTGCAGTGACATGTA CGAGAAAGTGCTGTGCACCAGCTGTGCCATGTTCGAGACCTGAGGCTGGCTCAAGCCGGCTGCCTTCACCGGGAGCCACGCCGTGCATGGCAGCCTTCCC TGGACGAGCGCTCGGTGTTCACACTGAACTGTGGGGTCGACGGGAGGGGTGCCTTTTACATGTTCTATTTTGTATCCTAATGACAGAATGAATAAACCTC TTTATATTTGCACAA

SIVA_HUMAN-3 (forward primer)
SIVA_HUMAN-4 (reverse primer)
Intron retention

Frame 0

CDVTASLVRGWRPGSCVAPGPAAMPKRSCPFAD VAPLQLKVRVSQRELSRGVCAERYSQEVFEKTKR LLFLGAQAYLDHVWDEGCAVVHLPESPKPGPTG APRAARGQMLIGPDGRLIRSLGQASEAGEWHQQ PFVAVALRAGGGTSIFLDLQGQAFPPWVSRTLTQ SLAFWLRRLLLSGGLTFKTGSC*TASKYWAKKA AETPRVSRLLSACSPLR*SHKAGNMPVPSLCSV GSQSITCVAG*KCNPRLYLKSGWCV*EEPRHLFV NQ*GSGPH*DPDGTHGVLTSNRGRMVLPALASR *QRDLVRERALNLESTTGISHLGSL**NGGPVH CTRLTGCQKDPV*VGMG*AGQSPLPSGMHGGR*P GSPGNSSLLAA*RLGVSSSQTHLG*RPLPVPHA CEPWMGRRSAVSVSEPCAGSVCAPAGAAAPWPV PCVASWSKYFSPWSC*DPIAPASRDGGRVGHPCG PRTHTCGP*CSAIWHCLTGHGGNRCLAQL*WLV ILSKGKLNLTDDLLCIRL*SLSSATSCSDL*AP PPPPPLVFEPPLPPSEEPLVGDTGAHRSFTTVMR AVWL*TLSGRKWSVCVVSHLWVGLLQLAGSRPY S*GGEGRSHPRGYSPGGWEWTVWSLFALPREKD DFIQTHVELAP*TQSKLSPNNV*NCPSKIETTCY ISSPFSRAFLQSGQRPRLIGDGEPWAGGAAPGR SAACQ*SAPMASAWAGVG*RTPSRLGIICRASY ECGILCLCFSPAEFIFFPRSKLKRAGFSHCLVAL SPKQK*T*PWAGSAERWVPERCVAGLPLLPCTP SLRDSLAPISSWPLPTFCCCFLPYPQSSR*AEW ATVPTVDTLFYRSYSTGGGLCPLSQINDLFQVTS QKWHSQARQPSWWHPQSPTPSPAISYFRTRRRR SGGKQGSVHTALV*HRCSPAAFGVLLMACTGPR LARPLQGSPAGCRWR*GAVFAAHPQYYWQTLQTK WQAVLAALLTPRPCRVWGSHCQSRFLATSLLIT FSLCFAGFPSLYL*SFLFFFFLRRSLALSPRLE CSGAILAHCNFHLLCSSNFPVSASQVAGVTGVR* HAQLIFVFLVETEFHHVGQAGLELLTSGDPPAS ASQSAEVSHTIRVSHHARPYP*S*YIRKALFDV LQCKPLWFGEFWQVIWRFLWRASILLETQVGPHV D*SAVGIS*GCCN*VRRPSGIDSKEVLPRGFGD QKSERKVLAGLVPSEAAMENLSWPLSWHLLVIF GVPCRQLPPPCIFMSSSFSVSFSSRSLFRTLVVL P*GPHVSAVI*TDDARSDPVSR*GHVLRCSGLG L*RLNGGGWWTQFSP*QQLFSPLPDAVAAAVTC TRKCCAPAVPCSRPEAGSSRLPSPGATPCMAAFP GRALGVHTELWGRREGCLLHVLFCILMTE*INL FIFAQ

Frame 1

VTSRRRW*GAGGRGAA*LPAPRPCPSGAAPSRT WPRYSSRSA*ARGS*AAACAPSATRRRSSRRPS DSCSSGPRPTWTTCGMKAVPSFTCQSPQSLALQG PRGLHVGRC*LDQTAA*SGALGRPPKLVSGTSS PLWLWH*GQVVARAFFLICKVRLFLPG*AGL*P RAWPSGSEGSCYQEVSHSRLEVVKQLLSTGPKKQ QRLPGYPVCFPPAAPSDSPTRLETCQSHPYAVW GLRASPVLLVRNAIPDSTSSLVGVSRRSPGTSL LTSKAVGHTETQMGHMEF*QATGEEWSFQH*PPG SRGTW*GKEH*TWSQLQAYLTLDLCSKTGVLCT VPGSRVVRRIQCRWGWGRQVRALCPQVCMEAGS PAVPETPVSLQPDGLAFLPHRPIWGSVHCLFLMR ASRGWEGGLRSV*ASPVRAVCAHLLGLRLRGLY PVWPRGVSTSVPGAAEIP*PQQAVMGDGWVTHV GPEHTRVAPDAVPSGIA*RDMVAIDAWPNFSG** SSLRES*TSQMTCSVSGSSPCLQPLPVLIFEHP HLLLLWSLSPPSPRPRSPWLVTQVHTAASPQSC VPFGCRPSQAGSGVFV*SPICGWVCCSWLDPVLT PKVGRGVLTLGATHLVDGSGQCGACLPCPVRKM TSSRHMWNWLHRPKASLVQTMSEIVHLK*KPHV TSRVLSLEPFFSRGRGPDSLETESPGQGGQHQGE APRASDRLPWLQHGQEWGNAPHPGWESSAGLAM NVGYSVFAFHPLSSSSFLEVN*KGQGFLIVWWP SHQSRSEHSHGQVQRRGGSQSAAWLASHSCPAHH P*GTPLRPFPPGLYLLSAAASSHTHRVHVELSG PLSPLWTHSFIAATAQEVGSALFHK*MTCSRSQ ARSGIPRPGSPAGGTRSLLPPPLPFPTSGPGVAG VGENRDLSTRLWFNTDAAQQPLASC*WPALAHA WPVLCRALQLAAGGGEGLSSRLTHNTTGKLCKP SGKQFWLPC*LHGPVVFGVAIASPVSSQHLS*SL SPCVLPGSLAFTFRAFFFFFF*DGVSLCHPGWS AVVRSWLTATSTSCVQAIFLSQPPK*LELQACA STPS*FLYS**RQSFTMLARLVSNS*PQVILRPQ PPKVLR*ATPYA*ATMPGPTLSLDISEKHCLMC FSVNHCGSVNSGRSSGGFFGELLSYWKPRLGPM WIDRQWESARAAVTEFADPVA*TAKRYCHVALET RSLRGRCWQGWFLLRLQWKTCPGLSPGICWLSL VFPVDSCPLPVSSCRLPSLCPSLHVVFLGRWSC CLRAPMSVPSSRLMMPAVILSPDKVMS*GVLG*D FDV*MVGGGGHNSAPDSSFSLPSLTLSQLQ*HV RESAVHQLCHVRDLRLAQAGCLHREPRRAWQPS LDERSVFTLNCGVDGRGAFYMFYFVS**QNE*TS LYLH

Frame 2

*RHGVVGKGLAAGELRSSRPRGHAQAELPLRGR GPATAQGPREPEGVEPRRVRRALLAGGLREDQA TPVPRGPGLPGPRVG*RLCRRSPARVPKAWPYR GPEGCTWADADWTRRPPDQEPWAGLRSW*VAPAA LCGCGTEGRWWHEHFS*FARSGFSSLGKQDSDP ELGLLAQKAPVIRRSHIQDWKLLNSF*VLGQKS SRDSPGIPSAFRLQPPQIVPQGWKHASPIPMQCG VSEHHLCCWLEMQSPTLPQVWLVCLGGAQAPLC *PVRQWATLRPRWDTWSSDKQQGKNGPSSTSLQ VAEGPGKGKSTELGVNYRHISPWISVVKRGSCAL YQAHGLSEGSSVGGDGVGRSEPSALRYAWRQVA RQSRKLQSPCSLTAWRFFLTDPSGVASIACSSC VRAVDGKAVCGQCERALCGQCVRTCWGCGSVACT LCGLVE*VLQSLELLRSHSPSKP*WGTGGSPMW AQNTHVWPLMQCHLALPNGTWWQ*MLGPTLVVS NPL*GKAEPHR*PALYPALVPVFSHFLF*SLSTP TSSSSGL*APPPPVRGAPGW*HRCTPQLHHSHA CRLVVDPLRQEVECLCSLPFVGGFAAAGWIPSL LLRWGGAFSP*GLLTWWMGVDSVEPVCPAP*ER* LHPDTCGTGSIDPKQA*SKQCLKLSI*NRNHML HLESFLSSLSSVGAEAQTHWRRRALGRGGSTRE KRRVPVIGSHGFSMGRSGVTHPIQVGNHLQG*L* MWDTLSLLFTR*VHLLS*K*TEKGRVFSLSGGP LTKAEVNIAMGRFSGEVGPRALRGWPPTPALHT ILEGLPCAHFLLASTYFLLLLPPIPTEFTLS*VG HCPHCGHTLLSQLQHRRWALPSFTNK*LVPGHK PEVAFPGQAAQLVAPAVSYPLPCHFLLQDQASQ EWGKTGICPHGSGLTQMQPSSLWRPADGLHWPTP GPSSAGLSSWLPVAVRGCLRGSPTILLANSANQ VASSSGCLVDSTALSCLG*PLPVPFPRNISLNH FLPVFCRVP*PLPLELSFFFFFETESRSVTQAGV QWCDLGSLQLPPPVFKQFSCLSLPSSWSYRRAL ARPANFCILSRDRVSPCWPGWSRTPDLR*SSGL SLPKC*GKPHHTREPPCPALPLVLIYQKSTV*CA SV*TIVVR*ILAGHLEVSLESFYPTGNPGWAPC GLIGSGNQLGLL*LSSQTQWHRQQRGTATWLWR PEV*EEGAGRAGSF*GCNGKPVLASLLASAGYLW CSL*TAAPSLYLHVVFLLCVLLFT*SF*DAGRV ALGPPCQCRHLD**CPQ*SCLQIRSCPEVFWVR TLTFEWWGVVDTIQPLTAAFLSPP*RCRSCSDMY EKVLCTSCAMFET*GWLKPAAFTGSHAVHGSLP WTSARCSH*TVGSTGGVPFTCSILYPNDRMNKP LYICT