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Sequences

Sequence TIAF1.fAug05

AGCCCAGTAACAAGATGGATGGGGCAGAGAAGGAGCCAACACAGTCATTTTGGTTTGGAATTACAGGCTAATAGATTCATTCATTTCCTGAAGCTGCTGT TGGATCTCTCTTCCCCTCCCCCAAGGAGGGGACTGGCTGTCGGGGCTGGATGCCCAGGGAGTGGGCAGTCCCAGGGAAAATGAGAGTCTAAGCCAGGAGA CCTTCGCAGACTTGAGGGGTCCTTCCCTGGGGCCTGAAGGGGGCAGAGTGCACGCAGGCCAGGGCCTGGACTGATAGTGCCAGGATGATGGGTCATTTGT TCTGGGCAGACCTTTTCTGCCTTCTCATTAGCCTCAAAGGAGCTGCCAGGAAGCAGCTGTCATTCAGCTCAGAGGCCCAGGGTCTGCCTGTACTGCACAC AGCCCTTTCCCTCATCTCCCTGGTCCAGAGCGGGACAGGCAGCTCCTTGAGCATCACTAGTCCTGGGACCCACCCCAGCAGCTGGAGGAGCAGCTGAGCC GCCTTCAGCGTGAGAAGAATGAGATCCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGC TTCCCGGGACCTGGCTCAGATAAATGATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAGAGC CAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAGGAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGA CGCAAGTGAAACGGCTGGAGAGCCTGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAACCGGGA GAAGGAACAGAACAAGCGGCTACAGAGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCAC GAACTGGAGATGGATCTAGAAAGCCTGGAGGCTGCTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCA TTGAGGATGAGATGGAGAGTGATGAGAATGAGGACCTCATCAACAGTGAGGGAGACTCTGATGTGGACTCGGAGCTGGAGGACCGTGTTGACGGGGTCAA GTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCAGCCCCACCAGCTACTGGAAGTCCCTTGCCCCT GATCGGTCAGATGATGAGCACGACCCTCTCGACAACACCTCCAGACCGCGATACTCCCACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGG AGACTAACGCATAGCCCAGGGGAGTGGTTGGCAGCCCTCTCACCCCAGGGCCTGTGGCTGCCTGGGCACCTCTCCCAGGAAGTGGTGGGGCACCGGTCTC CCCCACCCGACTGCTGATCTGCATGGGAAACACCCTGACCTTCTTCTGTCAGGGGCACTTTCCAGGCTATGGGTGTCTGATGTCTCCACGTGGAAGAGGT GGGGGAAAGAGGAGTTTCTGAAGAGAACTTTTTGCTCCTCTGTCTCAAAATGCCAGACTCTTGGCTTCTACCCTGTGTCACCGTGGGCAGTGGCAGGTGG CCTGGCACTGCATGGAGCCAGCACGTTGACCTCCCTCTCAGCTCCCTGCTCAGGGACGGTGGACAGGTTGCCTACTGGGACACTCTAGGTTGCTGGGTCC ATGGGGAGGATTGGGGGAGGAGAAGCAGTGCCTTCCCTCTCGTGTGGGGTGGGGGCTCTCTCTTCTTGGTGCCTGCTGTCTTTCTACTTTTTAATTTAAA TACCCAACCTCTCCATCACAGCTGCATCCCTGAGAGTGGGAGGGGGCTGTAGTGGTAGCTGGGGCTCCCAAGAACGACTCGGGAATGTCATCTCCATCTT CACCCTTCAGAGAGCAGTCCTTTCTCTGTGCAGCTGGAGACGCTGGTGAGGAGAGCCGGGTCCAGGTTCTTAAGAATGAGGTGCGGAGGGGCTCTCCGGT GCTGCTGGGCTGGGTTGAGCAAGCCTACGCAGACAAGTGTGTGTGTGGACCATCCGCACCTCCAGCCCCCACCCCACCCTCTTTGTCTCAGCGTGTTATG TGCAATGACCTATTTAAGGTAAACCCATTCCAACTACAGCAGTTCAGGGCTGATCCAAGCACTGCCTCCCTCCTGCTCTGTCCAGGTGGTCTGGACCATA AACTCAACTTGAGAGGGAAGGCTTGGGGTTGAGGACTTGTGATCAGAAAAACTGAAGATGGAAGTTTTGGCCGGTGCTCATTAGACATGAGTCCTCACTC TGTGTCCTGAGCCCGTGTCATTCTTCCAACCTCCCTGCCCCCACACACTTATCCCAGACACAACACCATGTGGTCTGGAGGTCCCAGCCCCCACCCTAAA AAGGTTATCCCTGAGAACTCCACCAGACTTGGGAGCCCAAGTGCAGTGCCTGGTGCTGCTCCCATCTGCCGCCCCCCTTCTCTCCTGCAATTGGTTTGTA CTCACTGGGCTGTGCTCTCCCCTGTTTACCCGATGTATGGAAATAAAGGCCCTTTTCCTCCTGGCTGCGCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

SPVTRWMGQRRSQHSHFGLELQANRFIHFLKLL LDLSSPPPRRGLAVGAGCPGSGQSQGK*ESKPGD LRRLEGSFPGA*RGQSARRPGPGLIVPG*WVIC SGQTFSAFSLASKELPGSSCHSAQRPRVCLYCT QPFPSSPWSRAGQAAP*ASLVLGPTPAAGGAAEP PSA*EE*DPEPAGGRSGRHERIDEEAQGCRGSG FPGPGSDK*SPSSARRSQQREAGAAGEATSPPE PGGVPGAVHGGQVPGEQAGS*DTGAGDTPGV*KD ASETAGEPG*PSQGKHGEAD*GAGSAHCSREPG EGTEQAATEAAPGHQGGDGRACQEGGRGEPQEA RTGDGSRKPGGC*PEPAG*PKVGIQAHRGPAGCH *G*DGE**E*GPHQQ*GRL*CGLGAGGPC*RGQ VLVVKKQGTFQGSF**WQLKEFQPHQLLEVPCP *SVR**ARPSRQHLQTAILPQLSE*QRHRGQADG D*RIAQGSGWQPSHPRACGCLGTSPRKWWGTGL PHPTADLHGKHPDLLLSGALSRLWVSDVSTWKR WGKEEFLKRTFCSSVSKCQTLGFYPVSPWAVAGG LALHGASTLTSLSAPCSGTVDRLPTGTL*VAGS MGRIGGGEAVPSLSCGVGALSSWCLLSFYFLI* IPNLSITAASLRVGGGCSGSWGSQERLGNVISIF TLQRAVLSLCSWRRW*GEPGPGS*E*GAEGLSG AAGLG*ASLRRQVCVWTIRTSSPHPTLFVSACY VQ*PI*GKPIPTTAVQG*SKHCLPPALSRWSGP* TQLEREGLGLRTCDQKN*RWKFWPVLIRHESSL CVLSPCHSSNLPAPTHLSQTQHHVVWRSQPPP* KGYP*ELHQTWEPKCSAWCCSHLPPPFSPAIGLY SLGCALPCLPDVWK*RPFSSWLRQ

Frame 1

AQ*QDGWGREGANTVILVWNYRLIDSFIS*SCC WISLPLPQGGDWLSGLDAQGVGSPRENESLSQE TFADLRGPSLGPEGGRVHAGQGLD**CQDDGSFV LGRPFLPSH*PQRSCQEAAVIQLRGPGSACTAH SPFPHLPGPERDRQLLEHH*SWDPPQQLEEQLS RLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQA SRDLAQINDLQAQLEEANKEKQELQEKLQALQS QVEFLEQSMVDKSLVSRQEAKIRELETRLEFER TQVKRLESLASRLKENMEKLTEERDQRIAAENRE KEQNKRLQRQLRDTKEEMGELARKEAEASRKKH ELEMDLESLEAANQSLQADLKLAFKRIGDLQAA IEDEMESDENEDLINSEGDSDVDSELEDRVDGVK SWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAP DRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLT ETNA*PRGVVGSPLTPGPVAAWAPLPGSGGAPVS PTRLLICMGNTLTFFCQGHFPGYGCLMSPRGRG GGKRSF*RELFAPLSQNARLLASTLCHRGQWQV AWHCMEPAR*PPSQLPAQGRWTGCLLGHSRLLGP WGGLGEEKQCLPSRVGWGLSLLGACCLSTF*FK YPTSPSQLHP*EWEGAVVVAGAPKNDSGMSSPS SPFREQSFLCAAGDAGEESRVQVLKNEVRRGSPV LLGWVEQAYADKCVCGPSAPPAPTPPSLSQRVM CNDLFKVNPFQLQQFRADPSTASLLLCPGGLDH KLNLRGKAWG*GLVIRKTEDGSFGRCSLDMSPHS VS*ARVILPTSLPPHTYPRHNTMWSGGPSPHPK KVIPENSTRLGSPSAVPGAAPICRPPSLLQLVC THWAVLSPVYPMYGNKGPFPPGCAS

Frame 2

PSNKMDGAEKEPTQSFWFGITG**IHSFPEAAV GSLFPSPKEGTGCRGWMPREWAVPGKMRV*ARR PSQT*GVLPWGLKGAECTQARAWTDSARMMGHL FWADLFCLLISLKGAARKQLSFSSEAQGLPVLHT ALSLISLVQSGTGSSLSITSPGTHPSSWRSS*A AFSVRRMRSRTGWRKIRKT*TN**RSTRLPWLR LPGTWLR*MISKLS*KKPTKRSRSCRRSYKPSRA RWSSWSSPWWTSPW*AGRKLRYGSWRHAWSLKG RK*NGWRAWLAVSRKTWRS*LRSGISALQPRTG RRNRTSGYRGSSGTPRRRWASLPGRRPRRAARST NWRWI*KAWRLLTRACRLT*SWHSSASGTCRLP LRMRWRVMRMRTSSTVRETLMWTRSWRTVLTGS SPGCQKTRDLPRQLLMMAA*RVPAPPATGSPLPL IGQMMSTTLSTTPPDRDTPTVI*VTATQRPS*R RLTHSPGEWLAALSPQGLWLPGHLSQEVVGHRS PPPDC*SAWETP*PSSVRGTFQAMGV*CLHVEEV GERGVSEENFLLLCLKMPDSWLLPCVTVGSGRW PGTAWSQHVDLPLSSLLRDGGQVAYWDTLGCWV HGEDWGRRSSAFPLVWGGGSLFLVPAVFLLFNLN TQPLHHSCIPESGRGL*W*LGLPRTTRECHLHL HPSESSPFSVQLETLVRRAGSRFLRMRCGGALR CCWAGLSKPTQTSVCVDHPHLQPPPHPLCLSVLC AMTYLR*THSNYSSSGLIQALPPSCSVQVVWTI NST*EGRLGVEDL*SEKLKMEVLAGAH*T*VLT LCPEPVSFFQPPCPHTLIPDTTPCGLEVPAPTLK RLSLRTPPDLGAQVQCLVLLPSAAPLLSCNWFV LTGLCSPLFTRCMEIKALFLLAAP

Sequence TIAF1.eAug05

ATCAGAGCGGAGTCTCCTTGCTCTGGGCTGCTCCAGGCCAGGCCATGCGGAAAGTGTGTTGTTATGAGGATTCAATGAGGAGACCTGTATGAAGTGCCTT CCCCTGTGCCTGGCTCATAGCGAAAACAGAAGAACAGATTGCAGCAGAAGAGGCCTGGAATGAGACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTC TCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTGCCTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGG ATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTCTGGAGGATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCA GCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCCCAGCCTGCTGGTTCTTGGCCCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCAC ATGTTCAAGGGTTGTCGGCGGGAGGACATGGCACCCCACATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGCCGTCAGGATCAGTCAA TCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCAGCTGCCAGCATCTGGTGCAGTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTT TTCTGTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTC TCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCCTCCATTCAGACAATGCTTCTGGAGAAGCTGCGTGTGGCTCGGCGCCCAGCCAGTGAAGCCA CATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATGGCACCCTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGT GCCACTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCAGCAGTTTAGTAAGCTGCAGGCGGCCATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAG GCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGGGCTGCGGGAGCCACCAAAGAAGCTGCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGT GGGCCCAGAAGGCTGCGTACCTACTGGGCTGCAGCCTGGAGGAGCTGTCCTCAGCCATCTTCAAGCACCAGCACAAGGGTGGCACCCTGCAGCGCTCCAC CTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGATGGGACAGGCCCGAAACTGAGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTAC AGCGAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGGGCTCTCAAGTCCAGCCAGCACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCC AGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCTTTGAGGAGCTGTGCCACAACTACACCCAAGACCGGCTGCAGAGGCTCTTCCACGAGCGCAC CTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACATCGAGCTGGCGTTTGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAG GCCTCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACAGACGAGGCGAGGGGCCTGCTCTGGCTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTG AGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCCCCCAGGAAGGTGACAAAAAAGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTT TCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTACAATGTGACTGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGG CTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTGTTTCTGGGCCGCGCAGGCAGTGCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCG GCTCGCAGCTGGCACTGCGCCGGGCCACCAGCATGCGGAAAACCTTTACCACAGGCATGGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCT ACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAAGCTGCATTTTGTGCACTGCTTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCC GCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCTCGGGAGACCACTGCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCA CCCAGCTCCGCGGCTCCCGCCTGCTCGATGCCATGCGCATGTACCGCCAAGGTTACCCTGACCACATGGTGTTTTCCGAGTTCCGCCGCCGCTTTGATGT CCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTACATCGTGGTGGATGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAG AGCAGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGGGCGGGCACCTTGGCACGGCTGGAGGAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCC TGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAA GAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTTTACCACAGTGAGGCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGAC GAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCGGAGAAGGAGAGGAACGAGCTGCGGCTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGC TGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAA GGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGATGGAGGTTATGGAAATGGAGGTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGG GAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTGAAGTATGAGCGGGCTGTGCGGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTG AGGACAAGCTGGAGGTGGAGCAGCAGAACAAGAGGCAGCTGGAACGGCGGCTCGGGGACCTGCAGGCAGATAGTGAGGAGAGTCAGCGGGCTCTGCAGCA GCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCAAGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAG AGGAGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAGGCCCAGCGGGAGAAGCTGCAGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTG AGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACATGGACATTGCAGGGTTCACCCAGAAGGTTGTGTCTCTAGAGGCAGAGCTCCAGGACATTTC TTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAACAGCTCCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAG CAGGCAGGGACCATCCAGATGCTGGAACAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGCGGATGAGACAGACCCATTCTAAGGAGATGGAGAGTCGGG ATGAGGAGGTGGAGGAGGCCCGGCAGTCGTGTCAGAAGAAGTTAAAACAGATGGAGGTGCAGCTAGAGGAAGAGTATGAGGACAAGCAGAAGGTTCTGCG AGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAGCGACCAGGTGAACCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGACCTGAAGCGC ACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGACCACCTGAAGAACAGTGCTCCCAGCAAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGG AGTCAGAGTTCACCTGTGCGGCAGCCGTGAAAGCACGGAAAGCAATGGAGGTGGAGATCGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAA GACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGAGAAGAATGAGATCCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAG AAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTGGCTCAGATAAATGATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGC AGGAGAAGCTACAAGCCCTCCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAGGAAGCTAAGATACGGGAGCT GGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACGGCTGGAGAGCCTGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGAT CAGCGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAACAAGCGGCTACAGAGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGG AGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGGATCTAGAAAGCCTGGAGGCTGCTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAA GCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGATGGAGAGTGATGAGAATGAGGACCTCATCAACAGTGAGGGAGACTCTGATGTGGACTCGGAG CTGGAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCAGCCCCA CCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATGATGAGCACGACCCTCTCGACAACACCTCCAGACCGCGATACTCCCACAGTTATCTGAGTGA CAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATAGCCCAGGGGAGTGGTTGGCAGCCCTCTCACCCCAGGGCCTGTGGCTGCCTGGGCACCTCTC CCAGGAAGTGGTGGGGCACCGGTCTCCCCCACCCGACTGCTGATCTGCATGGGAAACACCCTGACCTTCTTCTGTCAGGGGCACTTTCCAGGCTATGGGT GTCTGATGTCTCCACGTGGAAGAGGTGGGGGAAAGAGGAGTTTCTGAAGAGAACTTTTTGCTCCTCTGTCTCAAAATGCCAGACTCTTGGCTTCTACCCT GTGTCACCGTGGGCAGTGGCAGGTGGCCTGGCACTGCATGGAGCCAGCACGTTGACCTCCCTCTCAGCTCCCTGCTCAGGGACGGTGGACAGGTTGCCTA CTGGGACACTCTAGGTTGCTGGGTCCATGGGGAGGATTGGGGGAGGAGAAGCAGTGCCTTCCCTCTCGTGTGGGGTGGGGGCTCTCTCTTCTTGGTGCCT GCTGTCTTTCTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGCATCCCTGAGAGTGGGAGGGGGCTGTAGTGGTAGCTGGGGCTCCCAAGAA CGACTCGGGAATGTCATCTCCATCTTCACCCTTCAGAGAGCAGTCCTTTCTCTGTGCAGCTGGAGACGCTGGTGAGGAGAGCCGGGTCCAGGTTCTTAAG AATGAGGTGCGGAGGGGCTCTCCGGTGCTGCTGGGCTGGGTTGAGCAAGCCTACGCAGACAAGTGTGTGTGTGGACCATCCGCACCTCCAGCCCCCACCC CACCCTCTTTGTCTCAGCGTGTTATGTGCAATGACCTATTTAAGGTAAACCCATTCCAACTACAGCAGTTCAGGGCTGATCCAAGCACTGCCTCCCTCCT GCTCTGTCCAGGTGGTCTGGACCATAAACTCAACTTGAGAGGGAAGGCTTGGGGTTGAGGACTTGTGATCAGAAAAACTGAAGATGGAAGTTTTGGCCGG TGCTCATTAGACATGAGTCCTCACTCTGTGTCCTGAGCCCGTGTCATTCTTCCAACCTCCCTGCCCCCACACACTTATCCCAGACACAACACCATGTGGT CTGGAGGTCCCAGCCCCCACCCTAAAAAGGTTATCCCTGAGAACTCCACCAGACTTGGGAGCCCAAGTGCAGTGCCTGGTGCTGCTCCCATCTGCCGCCC CCCTTCTCTCCTGCAATTGGTTTGTACTCACTGGGCTGTGCTCTCCCCTGTTTACCCGATGTATGGAAATAAAGGCCCTTTTCCTCCTGGCTGCGCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

IRAESPCSGLLQARPCGKCVVMRIQ*GDLYEVP SPVPGS*RKQKNRLQQKRPGMRRRRCGWSIGTAS HWPVNSNLRSSTCLRGRCV*SWTTMGPSWMWMR MTLRRLMLPPATVWRIWPHWCTSMSPASCTPCA SAMALACCTRMLAPACWFLAPVGPLLCTLRR*CT CSRVVGGRTWHPTSMQWPRPHTGRC**AVRISQ SSSWAVVAVARPPAASIWCSTWPPSRASAGTRC FLWRSGRLCTPSWKPLGTAPPSLMAMPPASPRSS PWTLTKLARWPQPPFRQCFWRSCVWLGAQPVKP HSTSSTTCWPVGMAPSGQSSTSTTWQRTMCLGL CHWPSLRKSRRQLSSLVSCRRP*RCWASPPMNRR PAGSFWLPSTTWGLREPPKKLLKLGASSLPAMS GPRRLRTYWAAAWRSCPQPSSSTSTRVAPCSAP PPSARAPRRVAWEMGQARN*VHWSALRAWRPAST ASSSPFSSPW*IGLSSPASTHSAP**LSTPRAS RTLSRVGQPAEPPLRSCATTTPKTGCRGSSTSA PSCRSWKDTRRRTSSWRLTTWNPRRMTLWLLWTR PPISPWSARWPAQTRRGACSGYWKRRLWCQGPV RTPSWSAFSPIMAPRKVTKKAKAPFCTAANHTT FSWATAMAPTG*STM*LAG*TTPSRTQPPRMPPG SCRTPRKKSSATCFWAAQAVPRCSLAPSRAWRA ARSWHCAGPPACGKPLPQAWRLSKRSHCASR*S YRWTPSSTPSRSQSCILCTASCL*LRAGLGSPVP PPPAESAAAVSWTCPRETTARLGSCSSTCPCSA PSSAAPACSMPCACTAKVTLTTWCFPSSAAALM SWPRT*PRNTGVTTSWWMKGGQWRSCWSAWIWRR AAAAWA*AGCSSGRAPWHGWRSSGMNKPAGT*P CSKQPAGATWPASTSRRERSRTWPFAVYRRTSR RTKG*RTGPGGSFLPQ*GPSSKYSCQRSRSGTKT RRSSSCGASSRRRRRRGTSCGSTVTGWRAGSQS *HRS*QMSVTQESPPPSCWTRRQQRGSGLRRR* RNCRPSTMH*RSRWRLWKWR*WRPVSSGQRRSTG KWMMMMQVASGG*SMSGLCGRWTSPRNGSSRSL RTSWRWSSRTRGSWNGGSGTCRQIVRRVSGLCS SSRRSASD*RLSCKTPSCTWRASRSATTNWRRSR GGLTVSSRRRMRRPSGRSCSGRSCSGRRTCSSL RLSA*SSN*RKKTWTLQGSPRRLCL*RQSSRTF LPKSPRMRLLWPRSRNSSGTWRPKSRIRKKSWMS RQGPSRCWNRPSCVWRWRWSG*DRPILRRWRVG MRRWRRPGSRVRRS*NRWRCS*RKSMRTSRRFC ERSGSWRASSPPSATR*TGGTLSQRSGCGRT*SA PRPCWQMPSSCWTT*RTVLPASERLPSSRTSWR SQSSPVRQP*KHGKQWRWRSKTCTCRLMTSPKP RQRWRSS*AAFSVRRMRSRTGWRKIRKT*TN**R STRLPWLRLPGTWLR*MISKLS*KKPTKRSRSC RRSYKPSRARWSSWSSPWWTSPW*AGRKLRYGS WRHAWSLKGRK*NGWRAWLAVSRKTWRS*LRSGI SALQPRTGRRNRTSGYRGSSGTPRRRWASLPGR RPRRAARSTNWRWI*KAWRLLTRACRLT*SWHS SASGTCRLPLRMRWRVMRMRTSSTVRETLMWTRS WRTVLTGSSPGCQKTRDLPRQLLMMAA*RVPAP PATGSPLPLIGQMMSTTLSTTPPDRDTPTVI*V TATQRPS*RRLTHSPGEWLAALSPQGLWLPGHLS QEVVGHRSPPPDC*SAWETP*PSSVRGTFQAMG V*CLHVEEVGERGVSEENFLLLCLKMPDSWLLP CVTVGSGRWPGTAWSQHVDLPLSSLLRDGGQVAY WDTLGCWVHGEDWGRRSSAFPLVWGGGSLFLVP AVFLLFNLNTQPLHHSCIPESGRGL*W*LGLPR TTRECHLHLHPSESSPFSVQLETLVRRAGSRFLR MRCGGALRCCWAGLSKPTQTSVCVDHPHLQPPP HPLCLSVLCAMTYLR*THSNYSSSGLIQALPPS CSVQVVWTINST*EGRLGVEDL*SEKLKMEVLAG AH*T*VLTLCPEPVSFFQPPCPHTLIPDTTPCG LEVPAPTLKRLSLRTPPDLGAQVQCLVLLPSAA PLLSCNWFVLTGLCSPLFTRCMEIKALFLLAAP

Frame 1

SERSLLALGCSRPGHAESVLL*GFNEETCMKCL PLCLAHSENRRTDCSRRGLE*DGEGVAGP*GRL LTGQSTQI*GAQLA*GEGACEAGPRWGHPGCG*G *R*EG*CSLLRPSGGSGLTGVPQ*VQRPAHLAP ALWR*PAAHVCWPQPAGSWPPWGPCCVL*EGDA HVQGLSAGGHGTPHLCSGPDRIQGDADEPSGSVN HPPGQ*WQWQDHQLPASGAVPGHHRGHQREQGV FCGEVAGSVHPPGSLWEQPHHH*WQCHPLLPDP LPGL*PSWPGGLSLHSDNASGEAACGSAPSQ*SH IQRLLLPAGLWGWHPQDRAPPQPLGREQCVWDC ATGQA*GKAEGSSAV**AAGGHEGAGHLPR*TE GLLVHSGCHLPPGGCGSHQRSC*SWAQAVCPP*V GPEGCVPTGLQPGGAVLSHLQAPAQGWHPAALH LLPPGPRGEWPGRWDRPETECTGVP*GHGGRPL QRALHPSRLPGE*GSQVQPALTLLHDDCRHPGLP EP*AGWVSPRSLL*GAVPQLHPRPAAEALPRAH LRAGVGKIQGGEHRAGV*RLGTPDG*LCGCCGP GLPSVPGPLAGPHRRGEGPALAIGRGGSGARGQ* GHPPGAPFLLLWPPGR*QKRPKPPSAQQQTTPL SPGPQPWHQLGRVQCDWLAELHQAEPSHPECPP APAGLPEKNHQQPVSGPRRQCHGALWLHRGPGGR LAAGTAPGHQHAENLYHRHGGCQKEVTVHPDEA TGGRPHRHHQEVKAAFCALLPACS*GLGWGAPF RLLPPSQQQQ*AGPALGRPLRGWAPAARRAPAPH PAPRLPPARCHAHVPPRLP*PHGVFRVPPPL*C PGPAPDQETRA*LHRGG*KAGSGGAAGVLGSGE EQLLHGPEPGVLPGGHLGTAGGAAG*TNQQEPNP VPSSLQGLPGPPALQEEKDPGPGHSLCTEEHQE EQRGEGLALVEAFYHSEAPHRSTAVRGADPEQR RGDPAAAEQAREGGEGEERAAAQQ*PAGEPDLRA DIGADR*A*HRRVRLPAAGRGDSREAPG*EGDE GTADPVRCTEEADGGYGNGGDGGPSHPGSGDQR GSG***CRWRVAAEV*AGCAGGGLHQETAPAGV* GQAGGGAAEQEAAGTAARGPAGR**GESAGSAA AQEEVPATDG*AARHQAAPGGPAGPQPRTGEEA EEV*Q*ALAGA*GGPAGEAAAGEAAAGEGHAPR* GFQPEAATRGKRHGHCRVHPEGCVSRGRAPGHF FPRVQG*GFSGQGQETAPGPGGQSQGSGRRAG* AGRDHPDAGTGQAASGDGDGADETDPF*GDGESG *GGGGGPAVVSEEVKTDGGAARGRV*GQAEGSA REAGAGGQARHPQRPGEPAGL*VREAAAEGPEA HQGPAGRCPAHAGPPEEQCSQQARDCPAQEPAGG VRVHLCGSRESTESNGGGDRRPAPAD**HRQSQ DSAGGAAEPPSA*EE*DPEPAGGRSGRHERIDE EAQGCRGSGFPGPGSDK*SPSSARRSQQREAGAA GEATSPPEPGGVPGAVHGGQVPGEQAGS*DTGA GDTPGV*KDASETAGEPG*PSQGKHGEAD*GAG SAHCSREPGEGTEQAATEAAPGHQGGDGRACQEG GRGEPQEARTGDGSRKPGGC*PEPAG*PKVGIQ AHRGPAGCH*G*DGE**E*GPHQQ*GRL*CGLG AGGPC*RGQVLVVKKQGTFQGSF**WQLKEFQPH QLLEVPCP*SVR**ARPSRQHLQTAILPQLSE* QRHRGQADGD*RIAQGSGWQPSHPRACGCLGTS PRKWWGTGLPHPTADLHGKHPDLLLSGALSRLWV SDVSTWKRWGKEEFLKRTFCSSVSKCQTLGFYP VSPWAVAGGLALHGASTLTSLSAPCSGTVDRLP TGTL*VAGSMGRIGGGEAVPSLSCGVGALSSWCL LSFYFLI*IPNLSITAASLRVGGGCSGSWGSQE RLGNVISIFTLQRAVLSLCSWRRW*GEPGPGS* E*GAEGLSGAAGLG*ASLRRQVCVWTIRTSSPHP TLFVSACYVQ*PI*GKPIPTTAVQG*SKHCLPP ALSRWSGP*TQLEREGLGLRTCDQKN*RWKFWP VLIRHESSLCVLSPCHSSNLPAPTHLSQTQHHVV WRSQPPP*KGYP*ELHQTWEPKCSAWCCSHLPP PFSPAIGLYSLGCALPCLPDVWK*RPFSSWLRQ

Frame 2

QSGVSLLWAAPGQAMRKVCCYEDSMRRPV*SAF PCAWLIAKTEEQIAAEEAWNETEKVWLVHRDGF SLASQLKSEELNLPEGKVRVKLDHDGAILDVDE DDVEKANAPSCDRLEDLASLVYLNESSVLHTLRQ RYGASLLHTYAGPSLLVLGPRGAPAVYSEKVMH MFKGCRREDMAPHIYAVAQTAYRAMLMSRQDQS IILLGSSGSGKTTSCQHLVQYLATIAGISGNKVF SVEKWQALYTLLEAFGNSPTIINGNATRFSQIL SLDFDQAGQVASASIQTMLLEKLRVARRPASEA TFNVFYYLLACGDGTLRTELHLNHLAENNVFGIV PLAKPEEKQKAAQQFSKLQAAMKVLGISPDEQK ACWFILAAIYHLGAAGATKEAAEAGRKQFARHE WAQKAAYLLGCSLEELSSAIFKHQHKGGTLQRST SFRQGPEESGLGDGTGPKLSALECLEGMAAGLY SELFTLLVSLVNRALKSSQHSLCSMMIVDTPGF QNPEQGGSARGASFEELCHNYTQDRLQRLFHERT FVQELERYKEENIELAFDDLEPPTDDSVAAVDQ ASHQSLVRSLARTDEARGLLWLLEEEALVPGAS EDTLLERLFSYYGPQEGDKKGQSPLLHSSKPHHF LLGHSHGTNWVEYNVTGWLNYTKQNPATQNAPR LLQDSQKKIISNLFLGRAGSATVLSGSIAGLEG GSQLALRRATSMRKTFTTGMAAVKKKSLCIQMKL QVDALIDTIKKSKLHFVHCFLPVAEGWAGEPRS ASSRRVSSSSELDLPSGDHCEAGLLQLDVPLLR TQLRGSRLLDAMRMYRQGYPDHMVFSEFRRRFDV LAPHLTKKHGRNYIVVDERRAVEELLECLDLEK SSCCMGLSRVFFRAGTLARLEEQRDEQTSRNLT LFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKK NKGVKDWPWWKLFTTVRPLIEVQLSEEQIRNKD EEIQQLRSKLEKAEKERNELRLNSDRLESRISE LTSELTDERNTGESASQLLDAETAERLRAEKEMK ELQTQYDALKKQMEVMEMEVMEARLIRAAEING EVDDDDAGGEWRLKYERAVREVDFTKKRLQQEF EDKLEVEQQNKRQLERRLGDLQADSEESQRALQQ LKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQ RRFDSELSQAHEEAQREKLQREKLQREKDMLLA EAFSLKQQLEEKDMDIAGFTQKVVSLEAELQDIS SQESKDEASLAKVKKQLRDLEAKVKDQEEELDE QAGTIQMLEQAKLRLEMEMERMRQTHSKEMESR DEEVEEARQSCQKKLKQMEVQLEEEYEDKQKVLR EKRELEGKLATLSDQVNRRDFESEKRLRKDLKR TKALLADAQLMLDHLKNSAPSKREIAQLKNQLE ESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAK TALEEQLSRLQREKNEIQNRLEEDQEDMNELMK KHKAAVAQASRDLAQINDLQAQLEEANKEKQEL QEKLQALQSQVEFLEQSMVDKSLVSRQEAKIREL ETRLEFERTQVKRLESLASRLKENMEKLTEERD QRIAAENREKEQNKRLQRQLRDTKEEMGELARK EAEASRKKHELEMDLESLEAANQSLQADLKLAFK RIGDLQAAIEDEMESDENEDLINSEGDSDVDSE LEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSP TSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSD SDTEAKLTETNA*PRGVVGSPLTPGPVAAWAPL PGSGGAPVSPTRLLICMGNTLTFFCQGHFPGYG CLMSPRGRGGGKRSF*RELFAPLSQNARLLASTL CHRGQWQVAWHCMEPAR*PPSQLPAQGRWTGCL LGHSRLLGPWGGLGEEKQCLPSRVGWGLSLLGA CCLSTF*FKYPTSPSQLHP*EWEGAVVVAGAPKN DSGMSSPSSPFREQSFLCAAGDAGEESRVQVLK NEVRRGSPVLLGWVEQAYADKCVCGPSAPPAPT PPSLSQRVMCNDLFKVNPFQLQQFRADPSTASLL LCPGGLDHKLNLRGKAWG*GLVIRKTEDGSFGR CSLDMSPHSVS*ARVILPTSLPPHTYPRHNTMW SGGPSPHPKKVIPENSTRLGSPSAVPGAAPICRP PSLLQLVCTHWAVLSPVYPMYGNKGPFPPGCAS

Sequence TIAF1.dAug05

GCAGGGAGTCACCCGGCGCTGCGGAGCTCAGCACAGCCGGCCGGGAGGAGGCTCCCCCTGGAGCACGGGGCCAGGCCAGGTGCTGGCTACCCCTCCGGCC GCTGCTACAACCCTTGGCGAGGGGCAGAGCGCGGGGAGAGAGCCGGGGTCCCGGGGCTGGAGGAAACGGCCGCTCAGGGAGCAGCAAAGTGGGGCACCAA GGCCCTGTGCTAAGCACTCATAATCCTCTGGGGGTGCTACCCCTACAAACAGCACCCCCACCATGTTTAACCTAATGAAGAAAGACAAGGACAAAGATGG CGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGAAAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAGCCTGCGACGTGGCTTCTTC AACCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCCATCAAGGTGGCCAGCGGCTCTGACCTGCACCTGACTG ACATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACTCGGGCCACCTAAGTACAGCCAGCTCCAGCGATGACCTCAAGGGTGAGGAGGGTAGCTT CCGTGGCTCGGTGCTGCAGCGGGCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTCCTTCTCCCAGCGTAGCCGG GATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCTGCCGCCCCCTCGCCACAGGTGGAGGTGAGGACTCTAGAGGGACAGCTGGTGCAGCATC CTGGCCCAGGCATCCCTCGACCAGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAGTTCCCAGTCGACCTGCGCCTGCCCCCCGTGGTGCCCCT GCCCCCACCTACCCTCCGGGAGCTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCTCCCTGCGGCGCACAACCATGCTGGATCGGGGCCCCGAG GGCCAGGCCTGTCGGCGTGTGGTCCACTTTGCTGAGCCTGGTGCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGAGATCGACTGGTGGAGATTA ATGGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGGGACAGCGTGCGGCTCAAGGTGCAGCCCATTCCAGAGCT CAGCGAGCTCAGCAGGAGCTGGCTGCGGAGCGGCGAGGGACCTCGCAGGGAGCCATCCGATGCGAAAACAGAAGAACAGATTGCAGCAGAAGAGGCCTGG AATGAGACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTGCCTGAGGGGAAGGTGCGTG TGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGGATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTCTGGAGGATCTGGCCTCACT GGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCAGCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCCCAGCCTGCTGGTTCTTGGC CCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCGGGAGGACATGGCACCCCACATCTATGCAGTGGCCCAGA CCGCATACAGGGCGATGCTGATGAGCCGTCAGGATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCAGCTGCCAGCATCTGGTGCA GTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCTGTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGAAGCCTTTGGGAACAGCCCC ACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCCTCCATTCAGACAATGCTTC TGGAGAAGCTGCGTGTGGCTCGGCGCCCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATGGCACCCTCAGGACAGAGCT CCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCACTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCAGCAGTTTAGTAAGCTGCAG GCGGCCATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGGGCTGCGGGAGCCACCAAAG AAGCTGCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTACCTACTGGGCTGCAGCCTGGAGGAGCTGTCCTCAGCCAT CTTCAAGCACCAGCACAAGGGTGGCACCCTGCAGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGATGGGACAGGCCCGAAACTG AGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGCGAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGGGCTCTCAAGTCCAGCCAGC ACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCCAGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCTTTGAGGAGCTGTGCCACAA CTACACCCAAGACCGGCTGCAGAGGCTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACATCGAGCTGGCGTTTGACGAC TTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCCTCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACAGACGAGGCGAGGGGCCTGC TCTGGCTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCCCCCAGGAAGGTGACAAAAA AGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTTTCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTACAATGTGACTGGCTGGCTG AACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGGCTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTGTTTCTGGGCCGCGCAGGCA GTGCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGGGCCACCAGCATGCGGAAAACCTTTACCACAGGCAT GGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCTACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAAGCTGCATTTTGTGCACTGC TTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCTCGGGAGACCACT GCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCGCGGCTCCCGCCTGCTCGATGCCATGCGCATGTACCGCCAAGGTTACCC TGACCACATGGTGTTTTCCGAGTTCCGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTACATCGTGGTGGATGAAAGG CGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGCAGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGGGCGGGCACCTTGGCACGGC TGGAGGAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCCTGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGCACTTCAAGAAGAGAAAGAT CCAGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTTTACCACAGTGAGGCCCCTC ATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGACGAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCGGAGAAGGAGAGGAACGAGC TGCGGCTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGTCCGCCTCCCAGCTGCTGGA CGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAAGGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGATGGAGGTTATGGAAATGGAG GTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGGGAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTGAAGTATGAGCGGGCTGTGC GGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAGCAGCAGAACAAGAGGCAGCTGGAACGGCGGCTCGGGGA CCTGCAGGCAGATAGTGAGGAGAGTCAGCGGGCTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCAAGACACCAAGCTGCACCTG GAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAGGCCCAGCGGGAGAAGCTGC AGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTGAGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACATGGACATTGCAGGGTTCAC CCAGAAGGTTGTGTCTCTAGAGGCAGAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAACAGCTCCGGGAC CTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAGGCAGGGACCATCCAGATGCTGGAACAGTTAAAACAGATGGAGGTGCAGCTAGAGG AAGAGTATGAGGACAAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAGCGACCAGGTGAACCGGCGGGACTTTGAGTC AGAGAAGCGGCTGCGGAAGGACCTGAAGCGCACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGACCACCTGAAGAACAGTGCTCCCAGCAAGCGA GAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCAGCCGTGAAAGCACGGAAAGCAATGGAGGTGGAGATCGAAGACCTGC ACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGAGAAGAATGAGATCCAGAACCGGCTGGAGGA AGATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTGGCTCAGATAAATGATCTCCAAGCTCAGCTA GAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTGG TGAGCAGGCAGGAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACGGCTGGAGAGCCTGGCTAGCCGTCTCAAGGA AAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAACAAGCGGCTACAGAGGCAGCTCCGGGACACC AAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGGATCTAGAAAGCCTGGAGGCTGCTAACCAGA GCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGATGGAGAGTGATGAGAATGAGGACCTCATCAA CAGTGAGGGAGACTCTGATGTGGACTCGGAGCTGGAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCAGCTTCT GATGATGGCAGCTTAAAGAGTTCCAGCCCCACCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATGATGAGCACGACCCTCTCGACAACACCTCCA GACCGCGATACTCCCACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATAGCCCAGGGGAGTGGTTGGCAGCCCTCTCAC CCCAGGGCCTGTGGCTGCCTGGGCACCTCTCCCAGGAAGTGGTGGGGCACCGGTCTCCCCCACCCGACTGCTGATCTGCATGGGAAACACCCTGACCTTC TTCTGTCAGGGGCACTTTCCAGGCTATGGGTGTCTGATGTCTCCACGTGGAAGAGGTGGGGGAAAGAGGAGTTTCTGAAGAGAACTTTTTGCTCCTCTGT CTCAAAATGCCAGACTCTTGGCTTCTACCCTGTGTCACCGTGGGCAGTGGCAGGTGGCCTGGCACTGCATGGAGCCAGCACGTTGACCTCCCTCTCAGCT CCCTGCTCAGGGACGGTGGACAGGTTGCCTACTGGGACACTCTAGGTTGCTGGGTCCATGGGGAGGATTGGGGGAGGAGAAGCAGTGCCTTCCCTCTCGT GTGGGGTGGGGGCTCTCTCTTCTTGGTGCCTGCTGTCTTTCTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGCATCCCTGAGAGTGGGAGG GGGCTGTAGTGGTAGCTGGGGCTCCCAAGAACGACTCGGGAATGTCATCTCCATCTTCACCCTTCAGAGAGCAGTCCTTTCTCTGTGCAGCTGGAGACGC TGGTGAGGAGAGCCGGGTCCAGGTTCTTAAGAATGAGGTGCGGAGGGGCTCTCCGGTGCTGCTGGGCTGGGTTGAGCAAGCCTACGCAGACAAGTGTGTG TGTGGACCATCCGCACCTCCAGCCCCCACCCCACCCTCTTTGTCTCAGCGTGTTATGTGCAATGACCTATTTAAGGTAAACCCATTCCAACTACAGCAGT TCAGGGCTGATCCAAGCACTGCCTCCCTCCTGCTCTGTCCAGGTGGTCTGGACCATAAACTCAACTTGAGAGGGAAGGCTTGGGGTTGAGGACTTGTGAT CAGAAAAACTGAAGATGGAAGTTTTGGCCGGTGCTCATTAGACATGAGTCCTCACTCTGTGTCCTGAGCCCGTGTCATTCTTCCAACCTCCCTGCCCCCA CACACTTATCCCAGACACAACACCATGTGGTCTGGAGGTCCCAGCCCCCACCCTAAAAAGGTTATCCCTGAGAACTCCACCAGACTTGGGAGCCCAAGTG CAGTGCCTGGTGCTGCTCCCATCTGCCGCCCCCCTTCTCTCCTGCAATTGGTTTGTACTCACTGGGCTGTGCTCTCCCCTGTTTACCCGATGTATGGAAA TAAAGGCCCTTTTCCTCCTGGCTGCGCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

AGSHPALRSSAQPAGRRLPLEHGARPGAGYPSG RCYNPWRGAERGERAGVPGLEETAAQGAAKWGTK ALC*ALIILWGCYPYKQHPHHV*PNEERQGQRW RAEGEEGEKGEKGADVSGRASEPGGDEPATWLL QPEPLLQA*IQDAPGNLQPHPHQGGQRL*PAPD* H*LR**PGQRHPGLGPPKYSQLQR*PQG*GG*L PWLGAAAGSQVRLTGQAELTDDCQALFLLPA*P G*ERLRNLDALRALCRPLATGGGEDSRGTAGAAS WPRHPSTRAPIPSP*ASD*KVPSRPAPAPRGAP APTYPPGAGAATTAHWRLWLLPAAHNHAGSGPR GPGLSACGPLC*AWCRHQGPGPGAGARRSTGGD* WAQCGEQVQG*DCGDDPAVRGQRAAQGAAHSRA QRAQQELAAERRGTSQGAIRCENRRTDCSRRGL E*DGEGVAGP*GRLLTGQSTQI*GAQLA*GEGAC EAGPRWGHPGCG*G*R*EG*CSLLRPSGGSGLT GVPQ*VQRPAHLAPALWR*PAAHVCWPQPAGSW PPWGPCCVL*EGDAHVQGLSAGGHGTPHLCSGPD RIQGDADEPSGSVNHPPGQ*WQWQDHQLPASGA VPGHHRGHQREQGVFCGEVAGSVHPPGSLWEQP HHH*WQCHPLLPDPLPGL*PSWPGGLSLHSDNAS GEAACGSAPSQ*SHIQRLLLPAGLWGWHPQDRA PPQPLGREQCVWDCATGQA*GKAEGSSAV**AA GGHEGAGHLPR*TEGLLVHSGCHLPPGGCGSHQR SC*SWAQAVCPP*VGPEGCVPTGLQPGGAVLSH LQAPAQGWHPAALHLLPPGPRGEWPGRWDRPET ECTGVP*GHGGRPLQRALHPSRLPGE*GSQVQPA LTLLHDDCRHPGLPEP*AGWVSPRSLL*GAVPQ LHPRPAAEALPRAHLRAGVGKIQGGEHRAGV*R LGTPDG*LCGCCGPGLPSVPGPLAGPHRRGEGPA LAIGRGGSGARGQ*GHPPGAPFLLLWPPGR*QK RPKPPSAQQQTTPLSPGPQPWHQLGRVQCDWLA ELHQAEPSHPECPPAPAGLPEKNHQQPVSGPRRQ CHGALWLHRGPGGRLAAGTAPGHQHAENLYHRH GGCQKEVTVHPDEATGGRPHRHHQEVKAAFCAL LPACS*GLGWGAPFRLLPPSQQQQ*AGPALGRPL RGWAPAARRAPAPHPAPRLPPARCHAHVPPRLP *PHGVFRVPPPL*CPGPAPDQETRA*LHRGG*K AGSGGAAGVLGSGEEQLLHGPEPGVLPGGHLGTA GGAAG*TNQQEPNPVPSSLQGLPGPPALQEEKD PGPGHSLCTEEHQEEQRGEGLALVEAFYHSEAP HRSTAVRGADPEQRRGDPAAAEQAREGGEGEERA AAQQ*PAGEPDLRADIGADR*A*HRRVRLPAAG RGDSREAPG*EGDEGTADPVRCTEEADGGYGNG GDGGPSHPGSGDQRGSG***CRWRVAAEV*AGCA GGGLHQETAPAGV*GQAGGGAAEQEAAGTAARG PAGR**GESAGSAAAQEEVPATDG*AARHQAAP GGPAGPQPRTGEEAEEV*Q*ALAGA*GGPAGEAA AGEAAAGEGHAPR*GFQPEAATRGKRHGHCRVH PEGCVSRGRAPGHFFPRVQG*GFSGQGQETAPG PGGQSQGSGRRAG*AGRDHPDAGTVKTDGGAARG RV*GQAEGSAREAGAGGQARHPQRPGEPAGL*V REAAAEGPEAHQGPAGRCPAHAGPPEEQCSQQA RDCPAQEPAGGVRVHLCGSRESTESNGGGDRRPA PAD**HRQSQDSAGGAAEPPSA*EE*DPEPAGG RSGRHERIDEEAQGCRGSGFPGPGSDK*SPSSA RRSQQREAGAAGEATSPPEPGGVPGAVHGGQVPG EQAGS*DTGAGDTPGV*KDASETAGEPG*PSQG KHGEAD*GAGSAHCSREPGEGTEQAATEAAPGH QGGDGRACQEGGRGEPQEARTGDGSRKPGGC*PE PAG*PKVGIQAHRGPAGCH*G*DGE**E*GPHQ Q*GRL*CGLGAGGPC*RGQVLVVKKQGTFQGSF **WQLKEFQPHQLLEVPCP*SVR**ARPSRQHLQ TAILPQLSE*QRHRGQADGD*RIAQGSGWQPSH PRACGCLGTSPRKWWGTGLPHPTADLHGKHPDL LLSGALSRLWVSDVSTWKRWGKEEFLKRTFCSSV SKCQTLGFYPVSPWAVAGGLALHGASTLTSLSA PCSGTVDRLPTGTL*VAGSMGRIGGGEAVPSLS CGVGALSSWCLLSFYFLI*IPNLSITAASLRVGG GCSGSWGSQERLGNVISIFTLQRAVLSLCSWRR W*GEPGPGS*E*GAEGLSGAAGLG*ASLRRQVC VWTIRTSSPHPTLFVSACYVQ*PI*GKPIPTTAV QG*SKHCLPPALSRWSGP*TQLEREGLGLRTCD QKN*RWKFWPVLIRHESSLCVLSPCHSSNLPAP THLSQTQHHVVWRSQPPP*KGYP*ELHQTWEPKC SAWCCSHLPPPFSPAIGLYSLGCALPCLPDVWK *RPFSSWLRQ

Frame 1

QGVTRRCGAQHSRPGGGSPWSTGPGQVLATPPA AATTLGEGQSAGREPGSRGWRKRPLREQQSGAP RPCAKHS*SSGGATPTNSTPTMFNLMKKDKDKDG GRKEKKEKKEKKERMSAAELRSLEEMSLRRGFF NLNRSSKRESKTRLEISNPIPIKVASGSDLHLT DIDSDSNRGSVILDSGHLSTASSSDDLKGEEGSF RGSVLQRAAKFGSLAKQNSQMIVKRFSFSQRSR DESASETSTPSEHSAAPSPQVEVRTLEGQLVQH PGPGIPRPGHRSRAPELVTKKFPVDLRLPPVVPL PPPTLRELELQRRPTGDFGFSLRRTTMLDRGPE GQACRRVVHFAEPGAGTKDLALGLVPGDRLVEI NGHNVESKSRDEIVEMIRQSGDSVRLKVQPIPEL SELSRSWLRSGEGPRREPSDAKTEEQIAAEEAW NETEKVWLVHRDGFSLASQLKSEELNLPEGKVR VKLDHDGAILDVDEDDVEKANAPSCDRLEDLASL VYLNESSVLHTLRQRYGASLLHTYAGPSLLVLG PRGAPAVYSEKVMHMFKGCRREDMAPHIYAVAQ TAYRAMLMSRQDQSIILLGSSGSGKTTSCQHLVQ YLATIAGISGNKVFSVEKWQALYTLLEAFGNSP TIINGNATRFSQILSLDFDQAGQVASASIQTML LEKLRVARRPASEATFNVFYYLLACGDGTLRTEL HLNHLAENNVFGIVPLAKPEEKQKAAQQFSKLQ AAMKVLGISPDEQKACWFILAAIYHLGAAGATK EAAEAGRKQFARHEWAQKAAYLLGCSLEELSSAI FKHQHKGGTLQRSTSFRQGPEESGLGDGTGPKL SALECLEGMAAGLYSELFTLLVSLVNRALKSSQ HSLCSMMIVDTPGFQNPEQGGSARGASFEELCHN YTQDRLQRLFHERTFVQELERYKEENIELAFDD LEPPTDDSVAAVDQASHQSLVRSLARTDEARGL LWLLEEEALVPGASEDTLLERLFSYYGPQEGDKK GQSPLLHSSKPHHFLLGHSHGTNWVEYNVTGWL NYTKQNPATQNAPRLLQDSQKKIISNLFLGRAG SATVLSGSIAGLEGGSQLALRRATSMRKTFTTGM AAVKKKSLCIQMKLQVDALIDTIKKSKLHFVHC FLPVAEGWAGEPRSASSRRVSSSSELDLPSGDH CEAGLLQLDVPLLRTQLRGSRLLDAMRMYRQGYP DHMVFSEFRRRFDVLAPHLTKKHGRNYIVVDER RAVEELLECLDLEKSSCCMGLSRVFFRAGTLAR LEEQRDEQTSRNLTLFQAACRGYLARQHFKKRKI QDLAIRCVQKNIKKNKGVKDWPWWKLFTTVRPL IEVQLSEEQIRNKDEEIQQLRSKLEKAEKERNE LRLNSDRLESRISELTSELTDERNTGESASQLLD AETAERLRAEKEMKELQTQYDALKKQMEVMEME VMEARLIRAAEINGEVDDDDAGGEWRLKYERAV REVDFTKKRLQQEFEDKLEVEQQNKRQLERRLGD LQADSEESQRALQQLKKKCQRLTAELQDTKLHL EGQQVRNHELEKKQRRFDSELSQAHEEAQREKL QREKLQREKDMLLAEAFSLKQQLEEKDMDIAGFT QKVVSLEAELQDISSQESKDEASLAKVKKQLRD LEAKVKDQEEELDEQAGTIQMLEQLKQMEVQLE EEYEDKQKVLREKRELEGKLATLSDQVNRRDFES EKRLRKDLKRTKALLADAQLMLDHLKNSAPSKR EIAQLKNQLEESEFTCAAAVKARKAMEVEIEDL HLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEE DQEDMNELMKKHKAAVAQASRDLAQINDLQAQL EEANKEKQELQEKLQALQSQVEFLEQSMVDKSL VSRQEAKIRELETRLEFERTQVKRLESLASRLKE NMEKLTEERDQRIAAENREKEQNKRLQRQLRDT KEEMGELARKEAEASRKKHELEMDLESLEAANQ SLQADLKLAFKRIGDLQAAIEDEMESDENEDLIN SEGDSDVDSELEDRVDGVKSWLSKNKGPSKAAS DDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTS RPRYSHSYLSDSDTEAKLTETNA*PRGVVGSPLT PGPVAAWAPLPGSGGAPVSPTRLLICMGNTLTF FCQGHFPGYGCLMSPRGRGGGKRSF*RELFAPL SQNARLLASTLCHRGQWQVAWHCMEPAR*PPSQL PAQGRWTGCLLGHSRLLGPWGGLGEEKQCLPSR VGWGLSLLGACCLSTF*FKYPTSPSQLHP*EWE GAVVVAGAPKNDSGMSSPSSPFREQSFLCAAGDA GEESRVQVLKNEVRRGSPVLLGWVEQAYADKCV CGPSAPPAPTPPSLSQRVMCNDLFKVNPFQLQQ FRADPSTASLLLCPGGLDHKLNLRGKAWG*GLVI RKTEDGSFGRCSLDMSPHSVS*ARVILPTSLPP HTYPRHNTMWSGGPSPHPKKVIPENSTRLGSPS AVPGAAPICRPPSLLQLVCTHWAVLSPVYPMYGN KGPFPPGCAS

Frame 2

RESPGAAELSTAGREEAPPGARGQARCWLPLRP LLQPLARGRARGESRGPGAGGNGRSGSSKVGHQ GPVLSTHNPLGVLPLQTAPPPCLT**RKTRTKM AGGRRRRRKRRKRSGCQRQSFGAWRR*ACDVASS T*TAPPSVNPRRAWKSPTPSPSRWPAALTCT*L TLTPIVTGAASSWTRAT*VQPAPAMTSRVRRVA SVARCCSGQPSSAHWPSRTHR*LSSAFPSPSVAG MRAPQKPRRPQSTLPPPRHRWR*GL*RDSWCSI LAQASLDQGTDPEPLS**LKSSQSTCACPPWCP CPHLPSGSWSCNDGPLETLASPCGAQPCWIGAPR ARPVGVWSTLLSLVQAPRTWPWGWCQEIDWWRL MGTMWRASPGMRLWR*SGSQGTACGSRCSPFQS SASSAGAGCGAARDLAGSHPMRKQKNRLQQKRPG MRRRRCGWSIGTASHWPVNSNLRSSTCLRGRCV *SWTTMGPSWMWMRMTLRRLMLPPATVWRIWPH WCTSMSPASCTPCASAMALACCTRMLAPACWFLA PVGPLLCTLRR*CTCSRVVGGRTWHPTSMQWPR PHTGRC**AVRISQSSSWAVVAVARPPAASIWC STWPPSRASAGTRCFLWRSGRLCTPSWKPLGTAP PSLMAMPPASPRSSPWTLTKLARWPQPPFRQCF WRSCVWLGAQPVKPHSTSSTTCWPVGMAPSGQS STSTTWQRTMCLGLCHWPSLRKSRRQLSSLVSCR RP*RCWASPPMNRRPAGSFWLPSTTWGLREPPK KLLKLGASSLPAMSGPRRLRTYWAAAWRSCPQP SSSTSTRVAPCSAPPPSARAPRRVAWEMGQARN* VHWSALRAWRPASTASSSPFSSPW*IGLSSPAS THSAP**LSTPRASRTLSRVGQPAEPPLRSCAT TTPKTGCRGSSTSAPSCRSWKDTRRRTSSWRLTT WNPRRMTLWLLWTRPPISPWSARWPAQTRRGAC SGYWKRRLWCQGPVRTPSWSAFSPIMAPRKVTK KAKAPFCTAANHTTFSWATAMAPTG*STM*LAG* TTPSRTQPPRMPPGSCRTPRKKSSATCFWAAQA VPRCSLAPSRAWRAARSWHCAGPPACGKPLPQA WRLSKRSHCASR*SYRWTPSSTPSRSQSCILCTA SCL*LRAGLGSPVPPPPAESAAAVSWTCPRETT ARLGSCSSTCPCSAPSSAAPACSMPCACTAKVT LTTWCFPSSAAALMSWPRT*PRNTGVTTSWWMKG GQWRSCWSAWIWRRAAAAWA*AGCSSGRAPWHG WRSSGMNKPAGT*PCSKQPAGATWPASTSRRER SRTWPFAVYRRTSRRTKG*RTGPGGSFLPQ*GPS SKYSCQRSRSGTKTRRSSSCGASSRRRRRRGTS CGSTVTGWRAGSQS*HRS*QMSVTQESPPPSCW TRRQQRGSGLRRR*RNCRPSTMH*RSRWRLWKWR *WRPVSSGQRRSTGKWMMMMQVASGG*SMSGLC GRWTSPRNGSSRSLRTSWRWSSRTRGSWNGGSG TCRQIVRRVSGLCSSSRRSASD*RLSCKTPSCTW RASRSATTNWRRSRGGLTVSSRRRMRRPSGRSC SGRSCSGRRTCSSLRLSA*SSN*RKKTWTLQGS PRRLCL*RQSSRTFLPKSPRMRLLWPRSRNSSGT WRPKSRIRKKSWMSRQGPSRCWNS*NRWRCS*R KSMRTSRRFCERSGSWRASSPPSATR*TGGTLS QRSGCGRT*SAPRPCWQMPSSCWTT*RTVLPASE RLPSSRTSWRSQSSPVRQP*KHGKQWRWRSKTC TCRLMTSPKPRQRWRSS*AAFSVRRMRSRTGWR KIRKT*TN**RSTRLPWLRLPGTWLR*MISKLS* KKPTKRSRSCRRSYKPSRARWSSWSSPWWTSPW *AGRKLRYGSWRHAWSLKGRK*NGWRAWLAVSR KTWRS*LRSGISALQPRTGRRNRTSGYRGSSGTP RRRWASLPGRRPRRAARSTNWRWI*KAWRLLTR ACRLT*SWHSSASGTCRLPLRMRWRVMRMRTSS TVRETLMWTRSWRTVLTGSSPGCQKTRDLPRQLL MMAA*RVPAPPATGSPLPLIGQMMSTTLSTTPP DRDTPTVI*VTATQRPS*RRLTHSPGEWLAALS PQGLWLPGHLSQEVVGHRSPPPDC*SAWETP*PS SVRGTFQAMGV*CLHVEEVGERGVSEENFLLLC LKMPDSWLLPCVTVGSGRWPGTAWSQHVDLPLS SLLRDGGQVAYWDTLGCWVHGEDWGRRSSAFPLV WGGGSLFLVPAVFLLFNLNTQPLHHSCIPESGR GL*W*LGLPRTTRECHLHLHPSESSPFSVQLET LVRRAGSRFLRMRCGGALRCCWAGLSKPTQTSVC VDHPHLQPPPHPLCLSVLCAMTYLR*THSNYSS SGLIQALPPSCSVQVVWTINST*EGRLGVEDL* SEKLKMEVLAGAH*T*VLTLCPEPVSFFQPPCPH TLIPDTTPCGLEVPAPTLKRLSLRTPPDLGAQV QCLVLLPSAAPLLSCNWFVLTGLCSPLFTRCME IKALFLLAAP

Sequence TIAF1.cAug05

AGCTGCGCCGCGCTGCGCTCTGCTCCATCCCTGACCGGGCCGAGGCTGCTGGATGCCGCGTCTCCGCTTCTGCTGCCTGCCGGGCGGGCTCCGGTGGCCG CAGCAAAGTGGGGCACCAAGGCCCTGTGCTAAGCACTCATAATCCTCTGGGGGTGCTACCCCTACAAACAGCACCCCCACCATGTTTAACCTAATGAAGA AAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGAAAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAG CCTGCGACGTGGCTTCTTCAACCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCCATCAAGGTGGCCAGCGGC TCTGACCTGCACCTGACTGACATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACTCGGGCCACCTAAGTACAGCCAGCTCCAGCGATGACCTCA AGGGTGAGGAGGGTAGCTTCCGTGGCTCGGTGCTGCAGCGGGCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTC CTTCTCCCAGCGTAGCCGGGATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCTGCCGCCCCCTCGCCACAGGTGGAGGTGAGGACTCTAGAG GGACAGCTGGTGCAGCATCCTGGCCCAGGCATCCCTCGACCAGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAGTTCCCAGTCGACCTGCGCC TGCCCCCCGTGGTGCCCCTGCCCCCACCTACCCTCCGGGAGCTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCTCCCTGCGGCGCACAACCAT GCTGGATCGGGGCCCCGAGGGCCAGGCCTGTCGGCGTGTGGTCCACTTTGCTGAGCCTGGTGCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGA GATCGACTGGTGGAGATTAATGGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGGGACAGCGTGCGGCTCAAGG TGCAGCCCATTCCAGAGCTCAGCGAGCTCAGCAGGAGCTGGCTGCGGAGCGGCGAGGGACCTCGCAGGGAGCCATCCGATGCGAAAACAGAAGAACAGAT TGCAGCAGAAGAGGCCTGGAATGAGACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTG CCTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGGATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTC TGGAGGATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCAGCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCC CAGCCTGCTGGTTCTTGGCCCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCGGGAGGACATGGCACCCCAC ATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGCCGTCAGGATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCA GCTGCCAGCATCTGGTGCAGTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCTGTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGA AGCCTTTGGGAACAGCCCCACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCC TCCATTCAGACAATGCTTCTGGAGAAGCTGCGTGTGGCTCGGCGCCCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATG GCACCCTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCACTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCA GCAGTTTAGTAAGCTGCAGGCGGCCATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGG GCTGCGGGAGCCACCAAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTACCTACTGGGCTGCAGCCTGGAGGAGCTGTCCT CAGCCATCTTCAAGCACCAGCACAAGGGTGGCACCCTGCAGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGATGGGACAGGCCC GAAACTGAGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGCGAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGGGCTCTCAAGTCC AGCCAGCACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCCAGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCTTTGAGGAGCTGT GCCACAACTACACCCAAGACCGGCTGCAGAGGCTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACATCGAGCTGGCGTT TGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCCTCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACAGACGAGGCGAGG GGCCTGCTCTGGCTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCCCCCAGGAAGGTG ACAAAAAAGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTTTCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTACAATGTGACTGG CTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGGCTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTGTTTCTGGGCCGC GCAGGCAGTGCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGGGCCACCAGCATGCGGAAAACCTTTACCA CAGGCATGGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCTACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAAGCTGCATTTTGT GCACTGCTTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCTCGGGA GACCACTGCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCGCGGCTCCCGCCTGCTCGATGCCATGCGCATGTACCGCCAAG GTTACCCTGACCACATGGTGTTTTCCGAGTTCCGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTACATCGTGGTGGA TGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGCAGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGGGCGGGCACCTTG GCACGGCTGGAGGAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCCTGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGCACTTCAAGAAGA GAAAGATCCAGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTTTACCACAGTGAG GCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGACGAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCGGAGAAGGAGAGG AACGAGCTGCGGCTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGTCCGCCTCCCAGC TGCTGGACGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAAGGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGATGGAGGTTATGGA AATGGAGGTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGGGAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTGAAGTATGAGCGG GCTGTGCGGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAGCAGCAGAACAAGAGGCAGCTGGAACGGCGGC TCGGGGACCTGCAGGCAGATAGTGAGGAGAGTCAGCGGGCTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCAAGACACCAAGCT GCACCTGGAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAGGCCCAGCGGGAG AAGCTGCAGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTGAGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACATGGACATTGCAG GGTTCACCCAGAAGGTTGTGTCTCTAGAGGCAGAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAACAGCT CCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAGGCAGGGACCATCCAGATGCTGGAACAGGCCAAGCTGCGTCTGGAGATG GAGATGGAGCGGATGAGACAGACCCATTCTAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAGGAGGCCCGGCAGTCGTGTCAGAAGAAGTTAAAACAGA TGGAGGTGCAGCTAGAGGAAGAGTATGAGGACAAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAGCGACCAGGTGAA CCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGACCTGAAGCGCACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGACCACCTGAAGAAC AGTGCTCCCAGCAAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCAGCCGTGAAAGCACGGAAAGCAATGGAGG TGGAGATCGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGAGAAGAATGAGAT CCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTGGCTCAGATAAAT GATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCA TGGTGGACAAGTCCCTGGTGAGCAGGCAGGAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACGGCTGGAGAGCCT GGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAACAAGCGGCTACAG AGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGGATCTAGAAAGCC TGGAGGCTGCTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGATGGAGAGTGATGA GAATGAGGACCTCATCAACAGTGAGGGAGACTCTGATGTGGACTCGGAGCTGGAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCAAAAAACAAGGGA CCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCAGCCCCACCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATGATGAGCACGACC CTCTCGACAACACCTCCAGACCGCGATACTCCCACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATAGCCCAGGGGAGT GGTTGGCAGCCCTCTCACCCCAGGGCCTGTGGCTGCCTGGGCACCTCTCCCAGGAAGTGGTGGGGCACCGGTCTCCCCCACCCGACTGCTGATCTGCATG GGAAACACCCTGACCTTCTTCTGTCAGGGGCACTTTCCAGGCTATGGGTGTCTGATGTCTCCACGTGGAAGAGGTGGGGGAAAGAGGAGTTTCTGAAGAG AACTTTTTGCTCCTCTGTCTCAAAATGCCAGACTCTTGGCTTCTACCCTGTGTCACCGTGGGCAGTGGCAGGTGGCCTGGCACTGCATGGAGCCAGCACG TTGACCTCCCTCTCAGCTCCCTGCTCAGGGACGGTGGACAGGTTGCCTACTGGGACACTCTAGGTTGCTGGGTCCATGGGGAGGATTGGGGGAGGAGAAG CAGTGCCTTCCCTCTCGTGTGGGGTGGGGGCTCTCTCTTCTTGGTGCCTGCTGTCTTTCTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGC ATCCCTGAGAGTGGGAGGGGGCTGTAGTGGTAGCTGGGGCTCCCAAGAACGACTCGGGAATGTCATCTCCATCTTCACCCTTCAGAGAGCAGTCCTTTCT CTGTGCAGCTGGAGACGCTGGTGAGGAGAGCCGGGTCCAGGTTCTTAAGAATGAGGTGCGGAGGGGCTCTCCGGTGCTGCTGGGCTGGGTTGAGCAAGCC TACGCAGACAAGTGTGTGTGTGGACCATCCGCACCTCCAGCCCCCACCCCACCCTCTTTGTCTCAGCGTGTTATGTGCAATGACCTATTTAAGGTAAACC CATTCCAACTACAGCAGTTCAGGGCTGATCCAAGCACTGCCTCCCTCCTGCTCTGTCCAGGTGGTCTGGACCATAAACTCAACTTGAGAGGGAAGGCTTG GGGTTGAGGACTTGTGATCAGAAAAACTGAAGATGGAAGTTTTGGCCGGTGCTCATTAGACATGAGTCCTCACTCTGTGTCCTGAGCCCGTGTCATTCTT CCAACCTCCCTGCCCCCACACACTTATCCCAGACACAACACCATGTGGTCTGGAGGTCCCAGCCCCCACCCTAAAAAGGTTATCCCTGAGAACTCCACCA GACTTGGGAGCCCAAGTGCAGTGCCTGGTGCTGCTCCCATCTGCCGCCCCCCTTCTCTCCTGCAATTGGTTTGTACTCACTGGGCTGTGCTCTCCCCTGT TTACCCGATGTATGGAAATAAAGGCCCTTTTCCTCCTGGCTGCGCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

SCAALRSAPSLTGPRLLDAASPLLLPAGRAPVA AAKWGTKALC*ALIILWGCYPYKQHPHHV*PNEE RQGQRWRAEGEEGEKGEKGADVSGRASEPGGDE PATWLLQPEPLLQA*IQDAPGNLQPHPHQGGQR L*PAPD*H*LR**PGQRHPGLGPPKYSQLQR*PQ G*GG*LPWLGAAAGSQVRLTGQAELTDDCQALF LLPA*PG*ERLRNLDALRALCRPLATGGGEDSR GTAGAASWPRHPSTRAPIPSP*ASD*KVPSRPAP APRGAPAPTYPPGAGAATTAHWRLWLLPAAHNH AGSGPRGPGLSACGPLC*AWCRHQGPGPGAGAR RSTGGD*WAQCGEQVQG*DCGDDPAVRGQRAAQG AAHSRAQRAQQELAAERRGTSQGAIRCENRRTD CSRRGLE*DGEGVAGP*GRLLTGQSTQI*GAQL A*GEGACEAGPRWGHPGCG*G*R*EG*CSLLRPS GGSGLTGVPQ*VQRPAHLAPALWR*PAAHVCWP QPAGSWPPWGPCCVL*EGDAHVQGLSAGGHGTP HLCSGPDRIQGDADEPSGSVNHPPGQ*WQWQDHQ LPASGAVPGHHRGHQREQGVFCGEVAGSVHPPG SLWEQPHHH*WQCHPLLPDPLPGL*PSWPGGLS LHSDNASGEAACGSAPSQ*SHIQRLLLPAGLWGW HPQDRAPPQPLGREQCVWDCATGQA*GKAEGSS AV**AAGGHEGAGHLPR*TEGLLVHSGCHLPPG GCGSHQSWAQAVCPP*VGPEGCVPTGLQPGGAVL SHLQAPAQGWHPAALHLLPPGPRGEWPGRWDRP ETECTGVP*GHGGRPLQRALHPSRLPGE*GSQV QPALTLLHDDCRHPGLPEP*AGWVSPRSLL*GAV PQLHPRPAAEALPRAHLRAGVGKIQGGEHRAGV *RLGTPDG*LCGCCGPGLPSVPGPLAGPHRRGE GPALAIGRGGSGARGQ*GHPPGAPFLLLWPPGR* QKRPKPPSAQQQTTPLSPGPQPWHQLGRVQCDW LAELHQAEPSHPECPPAPAGLPEKNHQQPVSGP RRQCHGALWLHRGPGGRLAAGTAPGHQHAENLYH RHGGCQKEVTVHPDEATGGRPHRHHQEVKAAFC ALLPACS*GLGWGAPFRLLPPSQQQQ*AGPALG RPLRGWAPAARRAPAPHPAPRLPPARCHAHVPPR LP*PHGVFRVPPPL*CPGPAPDQETRA*LHRGG *KAGSGGAAGVLGSGEEQLLHGPEPGVLPGGHL GTAGGAAG*TNQQEPNPVPSSLQGLPGPPALQEE KDPGPGHSLCTEEHQEEQRGEGLALVEAFYHSE APHRSTAVRGADPEQRRGDPAAAEQAREGGEGE ERAAAQQ*PAGEPDLRADIGADR*A*HRRVRLPA AGRGDSREAPG*EGDEGTADPVRCTEEADGGYG NGGDGGPSHPGSGDQRGSG***CRWRVAAEV*A GCAGGGLHQETAPAGV*GQAGGGAAEQEAAGTAA RGPAGR**GESAGSAAAQEEVPATDG*AARHQA APGGPAGPQPRTGEEAEEV*Q*ALAGA*GGPAG EAAAGEAAAGEGHAPR*GFQPEAATRGKRHGHCR VHPEGCVSRGRAPGHFFPRVQG*GFSGQGQETA PGPGGQSQGSGRRAG*AGRDHPDAGTGQAASGD GDGADETDPF*GDGESG*GGGGGPAVVSEEVKTD GGAARGRV*GQAEGSAREAGAGGQARHPQRPGE PAGL*VREAAAEGPEAHQGPAGRCPAHAGPPEE QCSQQARDCPAQEPAGGVRVHLCGSRESTESNGG GDRRPAPAD**HRQSQDSAGGAAEPPSA*EE*D PEPAGGRSGRHERIDEEAQGCRGSGFPGPGSDK *SPSSARRSQQREAGAAGEATSPPEPGGVPGAVH GGQVPGEQAGS*DTGAGDTPGV*KDASETAGEP G*PSQGKHGEAD*GAGSAHCSREPGEGTEQAAT EAAPGHQGGDGRACQEGGRGEPQEARTGDGSRKP GGC*PEPAG*PKVGIQAHRGPAGCH*G*DGE** E*GPHQQ*GRL*CGLGAGGPC*RGQVLVVKKQG TFQGSF**WQLKEFQPHQLLEVPCP*SVR**ARP SRQHLQTAILPQLSE*QRHRGQADGD*RIAQGS GWQPSHPRACGCLGTSPRKWWGTGLPHPTADLH GKHPDLLLSGALSRLWVSDVSTWKRWGKEEFLKR TFCSSVSKCQTLGFYPVSPWAVAGGLALHGAST LTSLSAPCSGTVDRLPTGTL*VAGSMGRIGGGE AVPSLSCGVGALSSWCLLSFYFLI*IPNLSITAA SLRVGGGCSGSWGSQERLGNVISIFTLQRAVLS LCSWRRW*GEPGPGS*E*GAEGLSGAAGLG*AS LRRQVCVWTIRTSSPHPTLFVSACYVQ*PI*GKP IPTTAVQG*SKHCLPPALSRWSGP*TQLEREGL GLRTCDQKN*RWKFWPVLIRHESSLCVLSPCHS SNLPAPTHLSQTQHHVVWRSQPPP*KGYP*ELHQ TWEPKCSAWCCSHLPPPFSPAIGLYSLGCALPC LPDVWK*RPFSSWLRQ

Frame 1

AAPRCALLHP*PGRGCWMPRLRFCCLPGGLRWP QQSGAPRPCAKHS*SSGGATPTNSTPTMFNLMK KDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMS LRRGFFNLNRSSKRESKTRLEISNPIPIKVASG SDLHLTDIDSDSNRGSVILDSGHLSTASSSDDL KGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS FSQRSRDESASETSTPSEHSAAPSPQVEVRTLE GQLVQHPGPGIPRPGHRSRAPELVTKKFPVDLR LPPVVPLPPPTLRELELQRRPTGDFGFSLRRTTM LDRGPEGQACRRVVHFAEPGAGTKDLALGLVPG DRLVEINGHNVESKSRDEIVEMIRQSGDSVRLK VQPIPELSELSRSWLRSGEGPRREPSDAKTEEQI AAEEAWNETEKVWLVHRDGFSLASQLKSEELNL PEGKVRVKLDHDGAILDVDEDDVEKANAPSCDR LEDLASLVYLNESSVLHTLRQRYGASLLHTYAGP SLLVLGPRGAPAVYSEKVMHMFKGCRREDMAPH IYAVAQTAYRAMLMSRQDQSIILLGSSGSGKTT SCQHLVQYLATIAGISGNKVFSVEKWQALYTLLE AFGNSPTIINGNATRFSQILSLDFDQAGQVASA SIQTMLLEKLRVARRPASEATFNVFYYLLACGD GTLRTELHLNHLAENNVFGIVPLAKPEEKQKAAQ QFSKLQAAMKVLGISPDEQKACWFILAAIYHLG AAGATKAGRKQFARHEWAQKAAYLLGCSLEELS SAIFKHQHKGGTLQRSTSFRQGPEESGLGDGTGP KLSALECLEGMAAGLYSELFTLLVSLVNRALKS SQHSLCSMMIVDTPGFQNPEQGGSARGASFEEL CHNYTQDRLQRLFHERTFVQELERYKEENIELAF DDLEPPTDDSVAAVDQASHQSLVRSLARTDEAR GLLWLLEEEALVPGASEDTLLERLFSYYGPQEG DKKGQSPLLHSSKPHHFLLGHSHGTNWVEYNVTG WLNYTKQNPATQNAPRLLQDSQKKIISNLFLGR AGSATVLSGSIAGLEGGSQLALRRATSMRKTFT TGMAAVKKKSLCIQMKLQVDALIDTIKKSKLHFV HCFLPVAEGWAGEPRSASSRRVSSSSELDLPSG DHCEAGLLQLDVPLLRTQLRGSRLLDAMRMYRQ GYPDHMVFSEFRRRFDVLAPHLTKKHGRNYIVVD ERRAVEELLECLDLEKSSCCMGLSRVFFRAGTL ARLEEQRDEQTSRNLTLFQAACRGYLARQHFKK RKIQDLAIRCVQKNIKKNKGVKDWPWWKLFTTVR PLIEVQLSEEQIRNKDEEIQQLRSKLEKAEKER NELRLNSDRLESRISELTSELTDERNTGESASQ LLDAETAERLRAEKEMKELQTQYDALKKQMEVME MEVMEARLIRAAEINGEVDDDDAGGEWRLKYER AVREVDFTKKRLQQEFEDKLEVEQQNKRQLERR LGDLQADSEESQRALQQLKKKCQRLTAELQDTKL HLEGQQVRNHELEKKQRRFDSELSQAHEEAQRE KLQREKLQREKDMLLAEAFSLKQQLEEKDMDIA GFTQKVVSLEAELQDISSQESKDEASLAKVKKQL RDLEAKVKDQEEELDEQAGTIQMLEQAKLRLEM EMERMRQTHSKEMESRDEEVEEARQSCQKKLKQ MEVQLEEEYEDKQKVLREKRELEGKLATLSDQVN RRDFESEKRLRKDLKRTKALLADAQLMLDHLKN SAPSKREIAQLKNQLEESEFTCAAAVKARKAME VEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEI QNRLEEDQEDMNELMKKHKAAVAQASRDLAQIN DLQAQLEEANKEKQELQEKLQALQSQVEFLEQS MVDKSLVSRQEAKIRELETRLEFERTQVKRLESL ASRLKENMEKLTEERDQRIAAENREKEQNKRLQ RQLRDTKEEMGELARKEAEASRKKHELEMDLES LEAANQSLQADLKLAFKRIGDLQAAIEDEMESDE NEDLINSEGDSDVDSELEDRVDGVKSWLSKNKG PSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHD PLDNTSRPRYSHSYLSDSDTEAKLTETNA*PRGV VGSPLTPGPVAAWAPLPGSGGAPVSPTRLLICM GNTLTFFCQGHFPGYGCLMSPRGRGGGKRSF*R ELFAPLSQNARLLASTLCHRGQWQVAWHCMEPAR *PPSQLPAQGRWTGCLLGHSRLLGPWGGLGEEK QCLPSRVGWGLSLLGACCLSTF*FKYPTSPSQL HP*EWEGAVVVAGAPKNDSGMSSPSSPFREQSFL CAAGDAGEESRVQVLKNEVRRGSPVLLGWVEQA YADKCVCGPSAPPAPTPPSLSQRVMCNDLFKVN PFQLQQFRADPSTASLLLCPGGLDHKLNLRGKAW G*GLVIRKTEDGSFGRCSLDMSPHSVS*ARVIL PTSLPPHTYPRHNTMWSGGPSPHPKKVIPENST RLGSPSAVPGAAPICRPPSLLQLVCTHWAVLSPV YPMYGNKGPFPPGCAS

Frame 2

LRRAALCSIPDRAEAAGCRVSASAACRAGSGGR SKVGHQGPVLSTHNPLGVLPLQTAPPPCLT**R KTRTKMAGGRRRRRKRRKRSGCQRQSFGAWRR* ACDVASST*TAPPSVNPRRAWKSPTPSPSRWPAA LTCT*LTLTPIVTGAASSWTRAT*VQPAPAMTS RVRRVASVARCCSGQPSSAHWPSRTHR*LSSAF PSPSVAGMRAPQKPRRPQSTLPPPRHRWR*GL*R DSWCSILAQASLDQGTDPEPLS**LKSSQSTCA CPPWCPCPHLPSGSWSCNDGPLETLASPCGAQP CWIGAPRARPVGVWSTLLSLVQAPRTWPWGWCQE IDWWRLMGTMWRASPGMRLWR*SGSQGTACGSR CSPFQSSASSAGAGCGAARDLAGSHPMRKQKNR LQQKRPGMRRRRCGWSIGTASHWPVNSNLRSSTC LRGRCV*SWTTMGPSWMWMRMTLRRLMLPPATV WRIWPHWCTSMSPASCTPCASAMALACCTRMLA PACWFLAPVGPLLCTLRR*CTCSRVVGGRTWHPT SMQWPRPHTGRC**AVRISQSSSWAVVAVARPP AASIWCSTWPPSRASAGTRCFLWRSGRLCTPSW KPLGTAPPSLMAMPPASPRSSPWTLTKLARWPQP PFRQCFWRSCVWLGAQPVKPHSTSSTTCWPVGM APSGQSSTSTTWQRTMCLGLCHWPSLRKSRRQL SSLVSCRRP*RCWASPPMNRRPAGSFWLPSTTWG LREPPKLGASSLPAMSGPRRLRTYWAAAWRSCP QPSSSTSTRVAPCSAPPPSARAPRRVAWEMGQA RN*VHWSALRAWRPASTASSSPFSSPW*IGLSSP ASTHSAP**LSTPRASRTLSRVGQPAEPPLRSC ATTTPKTGCRGSSTSAPSCRSWKDTRRRTSSWR LTTWNPRRMTLWLLWTRPPISPWSARWPAQTRRG ACSGYWKRRLWCQGPVRTPSWSAFSPIMAPRKV TKKAKAPFCTAANHTTFSWATAMAPTG*STM*L AG*TTPSRTQPPRMPPGSCRTPRKKSSATCFWAA QAVPRCSLAPSRAWRAARSWHCAGPPACGKPLP QAWRLSKRSHCASR*SYRWTPSSTPSRSQSCIL CTASCL*LRAGLGSPVPPPPAESAAAVSWTCPRE TTARLGSCSSTCPCSAPSSAAPACSMPCACTAK VTLTTWCFPSSAAALMSWPRT*PRNTGVTTSWW MKGGQWRSCWSAWIWRRAAAAWA*AGCSSGRAPW HGWRSSGMNKPAGT*PCSKQPAGATWPASTSRR ERSRTWPFAVYRRTSRRTKG*RTGPGGSFLPQ* GPSSKYSCQRSRSGTKTRRSSSCGASSRRRRRRG TSCGSTVTGWRAGSQS*HRS*QMSVTQESPPPS CWTRRQQRGSGLRRR*RNCRPSTMH*RSRWRLW KWR*WRPVSSGQRRSTGKWMMMMQVASGG*SMSG LCGRWTSPRNGSSRSLRTSWRWSSRTRGSWNGG SGTCRQIVRRVSGLCSSSRRSASD*RLSCKTPS CTWRASRSATTNWRRSRGGLTVSSRRRMRRPSGR SCSGRSCSGRRTCSSLRLSA*SSN*RKKTWTLQ GSPRRLCL*RQSSRTFLPKSPRMRLLWPRSRNS SGTWRPKSRIRKKSWMSRQGPSRCWNRPSCVWRW RWSG*DRPILRRWRVGMRRWRRPGSRVRRS*NR WRCS*RKSMRTSRRFCERSGSWRASSPPSATR* TGGTLSQRSGCGRT*SAPRPCWQMPSSCWTT*RT VLPASERLPSSRTSWRSQSSPVRQP*KHGKQWR WRSKTCTCRLMTSPKPRQRWRSS*AAFSVRRMR SRTGWRKIRKT*TN**RSTRLPWLRLPGTWLR*M ISKLS*KKPTKRSRSCRRSYKPSRARWSSWSSP WWTSPW*AGRKLRYGSWRHAWSLKGRK*NGWRA WLAVSRKTWRS*LRSGISALQPRTGRRNRTSGYR GSSGTPRRRWASLPGRRPRRAARSTNWRWI*KA WRLLTRACRLT*SWHSSASGTCRLPLRMRWRVM RMRTSSTVRETLMWTRSWRTVLTGSSPGCQKTRD LPRQLLMMAA*RVPAPPATGSPLPLIGQMMSTT LSTTPPDRDTPTVI*VTATQRPS*RRLTHSPGE WLAALSPQGLWLPGHLSQEVVGHRSPPPDC*SAW ETP*PSSVRGTFQAMGV*CLHVEEVGERGVSEE NFLLLCLKMPDSWLLPCVTVGSGRWPGTAWSQH VDLPLSSLLRDGGQVAYWDTLGCWVHGEDWGRRS SAFPLVWGGGSLFLVPAVFLLFNLNTQPLHHSC IPESGRGL*W*LGLPRTTRECHLHLHPSESSPF SVQLETLVRRAGSRFLRMRCGGALRCCWAGLSKP TQTSVCVDHPHLQPPPHPLCLSVLCAMTYLR*T HSNYSSSGLIQALPPSCSVQVVWTINST*EGRL GVEDL*SEKLKMEVLAGAH*T*VLTLCPEPVSFF QPPCPHTLIPDTTPCGLEVPAPTLKRLSLRTPP DLGAQVQCLVLLPSAAPLLSCNWFVLTGLCSPL FTRCMEIKALFLLAAP

Sequence TIAF1.bAug05

AGCTGCGCCGCGCTGCGCTCTGCTCCATCCCTGACCGGGCCGAGGCTGCTGGATGCCGCGTCTCCGCTTCTGCTGCCTGCCGGGCGGGCTCCGGTGGCCG CAGCAAAGTGGGGCACCAAGGCCCTGTGCTAAGCACTCATAATCCTCTGGGGGTGCTACCCCTACAAACAGCACCCCCACCATGTTTAACCTAATGAAGA AAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGAAAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAG CCTGCGACGTGGCTTCTTCAACCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCCATCAAGGTGGCCAGCGGC TCTGACCTGCACCTGACTGACATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACTCGGGCCACCTAAGTACAGCCAGCTCCAGCGATGACCTCA AGGGTGAGGAGGGTAGCTTCCGTGGCTCGGTGCTGCAGCGGGCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTC CTTCTCCCAGCGTAGCCGGGATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCTGCCGCCCCCTCGCCACAGGTGGAGGTGAGGACTCTAGAG GGACAGCTGGTGCAGCATCCTGGCCCAGGCATCCCTCGACCAGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAGTTCCCAGTCGACCTGCGCC TGCCCCCCGTGGTGCCCCTGCCCCCACCTACCCTCCGGGAGCTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCTCCCTGCGGCGCACAACCAT GCTGGATCGGGGCCCCGAGGGCCAGGCCTGTCGGCGTGTGGTCCACTTTGCTGAGCCTGGTGCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGA GATCGACTGGTGGAGATTAATGGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGGGACAGCGTGCGGCTCAAGG TGCAGCCCATTCCAGAGCTCAGCGAGCTCAGCAGGAGCTGGCTGCGGAGCGGCGAGGGACCTCGCAGGGAGCCATCCGATGCGAAAACAGAAGAACAGAT TGCAGCAGAAGAGGCCTGGAATGAGACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTG CCTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGGATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTC TGGAGGATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCAGCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCC CAGCCTGCTGGTTCTTGGCCCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCGGGAGGACATGGCACCCCAC ATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGCCGTCAGGATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCA GCTGCCAGCATCTGGTGCAGTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCTGTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGA AGCCTTTGGGAACAGCCCCACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCC TCCATTCAGACAATGCTTCTGGAGAAGCTGCGTGTGGCTCGGCGCCCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATG GCACCCTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCACTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCA GCAGTTTAGTAAGCTGCAGGCGGCCATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGG GCTGCGGGAGCCACCAAAGAAGCTGCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTACCTACTGGGCTGCAGCCTGG AGGAGCTGTCCTCAGCCATCTTCAAGCACCAGCACAAGGGTGGCACCCTGCAGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGA TGGGACAGGCCCGAAACTGAGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGCGAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGG GCTCTCAAGTCCAGCCAGCACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCCAGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCT TTGAGGAGCTGTGCCACAACTACACCCAAGACCGGCTGCAGAGGCTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACAT CGAGCTGGCGTTTGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCCTCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACA GACGAGGCGAGGGGCCTGCTCTGGCTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCC CCCAGGAAGGTGACAAAAAAGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTTTCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTA CAATGTGACTGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGGCTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTG TTTCTGGGCCGCGCAGGCAGTGCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGGGCCACCAGCATGCGGA AAACCTTTACCACAGGCATGGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCTACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAA GCTGCATTTTGTGCACTGCTTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGAC CTGCCCTCGGGAGACCACTGCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCGCGGCTCCCGCCTGCTCGATGCCATGCGCA TGTACCGCCAAGGTTACCCTGACCACATGGTGTTTTCCGAGTTCCGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTA CATCGTGGTGGATGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGCAGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGG GCGGGCACCTTGGCACGGCTGGAGGAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCCTGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGC ACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTT TACCACAGTGAGGCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGACGAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCG GAGAAGGAGAGGAACGAGCTGCGGCTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGT CCGCCTCCCAGCTGCTGGACGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAAGGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGAT GGAGGTTATGGAAATGGAGGTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGGGAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTG AAGTATGAGCGGGCTGTGCGGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAGCAGCAGAACAAGAGGCAGC TGGAACGGCGGCTCGGGGACCTGCAGGCAGATAGTGAGGAGAGTCAGCGGGCTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCA AGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAG GCCCAGCGGGAGAAGCTGCAGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTGAGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACA TGGACATTGCAGGGTTCACCCAGAAGGTTGTGTCTCTAGAGGCAGAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGT CAAGAAACAGCTCCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAGGCAGGGACCATCCAGATGCTGGAACAGGCCAAGCTG CGTCTGGAGATGGAGATGGAGCGGATGAGACAGACCCATTCTAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAGGAGGCCCGGCAGTCGTGTCAGAAGA AGTTAAAACAGATGGAGGTGCAGCTAGAGGAAGAGTATGAGGACAAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAG CGACCAGGTGAACCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGACCTGAAGCGCACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGAC CACCTGAAGAACAGTGCTCCCAGCAAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCAGCCGTGAAAGCACGGA AAGCAATGGAGGTGGAGATCGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGA GAAGAATGAGATCCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTG GCTCAGATAAATGATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAGAGCCAGGTGGAGTTCC TGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAGGAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACG GCTGGAGAGCCTGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAAC AAGCGGCTACAGAGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGG ATCTAGAAAGCCTGGAGGCTGCTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGAT GGAGAGTGATGAGAATGAGGACCTCATCAACAGTGAGGGAGACTCTGATGTGGACTCGGAGCTGGAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCA AAAAACAAGGGACCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCAGCCCCACCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATG ATGAGCACGACCCTCTCGACAACACCTCCAGACCGCGATACTCCCACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATA GCCCAGGGGAGTGGTTGGCAGCCCTCTCACCCCAGGGCCTGTGGCTGCCTGGGCACCTCTCCCAGGAAGTGGTGGGGCACCGGTCTCCCCCACCCGACTG CTGATCTGCATGGGAAACACCCTGACCTTCTTCTGTCAGGGGCACTTTCCAGGCTATGGGTGTCTGATGTCTCCACGTGGAAGAGGTGGGGGAAAGAGGA GTTTCTGAAGAGAACTTTTTGCTCCTCTGTCTCAAAATGCCAGACTCTTGGCTTCTACCCTGTGTCACCGTGGGCAGTGGCAGGTGGCCTGGCACTGCAT GGAGCCAGCACGTTGACCTCCCTCTCAGCTCCCTGCTCAGGGACGGTGGACAGGTTGCCTACTGGGACACTCTAGGTTGCTGGGTCCATGGGGAGGATTG GGGGAGGAGAAGCAGTGCCTTCCCTCTCGTGTGGGGTGGGGGCTCTCTCTTCTTGGTGCCTGCTGTCTTTCTACTTTTTAATTTAAATACCCAACCTCTC CATCACAGCTGCATCCCTGAGAGTGGGAGGGGGCTGTAGTGGTAGCTGGGGCTCCCAAGAACGACTCGGGAATGTCATCTCCATCTTCACCCTTCAGAGA GCAGTCCTTTCTCTGTGCAGCTGGAGACGCTGGTGAGGAGAGCCGGGTCCAGGTTCTTAAGAATGAGGTGCGGAGGGGCTCTCCGGTGCTGCTGGGCTGG GTTGAGCAAGCCTACGCAGACAAGTGTGTGTGTGGACCATCCGCACCTCCAGCCCCCACCCCACCCTCTTTGTCTCAGCGTGTTATGTGCAATGACCTAT TTAAGGTAAACCCATTCCAACTACAGCAGTTCAGGGCTGATCCAAGCACTGCCTCCCTCCTGCTCTGTCCAGGTGGTCTGGACCATAAACTCAACTTGAG AGGGAAGGCTTGGGGTTGAGGACTTGTGATCAGAAAAACTGAAGATGGAAGTTTTGGCCGGTGCTCATTAGACATGAGTCCTCACTCTGTGTCCTGAGCC CGTGTCATTCTTCCAACCTCCCTGCCCCCACACACTTATCCCAGACACAACACCATGTGGTCTGGAGGTCCCAGCCCCCACCCTAAAAAGGTTATCCCTG AGAACTCCACCAGACTTGGGAGCCCAAGTGCAGTGCCTGGTGCTGCTCCCATCTGCCGCCCCCCTTCTCTCCTGCAATTGGTTTGTACTCACTGGGCTGT GCTCTCCCCTGTTTACCCGATGTATGGAAATAAAGGCCCTTTTCCTCCTGGCTGCGCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

SCAALRSAPSLTGPRLLDAASPLLLPAGRAPVA AAKWGTKALC*ALIILWGCYPYKQHPHHV*PNEE RQGQRWRAEGEEGEKGEKGADVSGRASEPGGDE PATWLLQPEPLLQA*IQDAPGNLQPHPHQGGQR L*PAPD*H*LR**PGQRHPGLGPPKYSQLQR*PQ G*GG*LPWLGAAAGSQVRLTGQAELTDDCQALF LLPA*PG*ERLRNLDALRALCRPLATGGGEDSR GTAGAASWPRHPSTRAPIPSP*ASD*KVPSRPAP APRGAPAPTYPPGAGAATTAHWRLWLLPAAHNH AGSGPRGPGLSACGPLC*AWCRHQGPGPGAGAR RSTGGD*WAQCGEQVQG*DCGDDPAVRGQRAAQG AAHSRAQRAQQELAAERRGTSQGAIRCENRRTD CSRRGLE*DGEGVAGP*GRLLTGQSTQI*GAQL A*GEGACEAGPRWGHPGCG*G*R*EG*CSLLRPS GGSGLTGVPQ*VQRPAHLAPALWR*PAAHVCWP QPAGSWPPWGPCCVL*EGDAHVQGLSAGGHGTP HLCSGPDRIQGDADEPSGSVNHPPGQ*WQWQDHQ LPASGAVPGHHRGHQREQGVFCGEVAGSVHPPG SLWEQPHHH*WQCHPLLPDPLPGL*PSWPGGLS LHSDNASGEAACGSAPSQ*SHIQRLLLPAGLWGW HPQDRAPPQPLGREQCVWDCATGQA*GKAEGSS AV**AAGGHEGAGHLPR*TEGLLVHSGCHLPPG GCGSHQRSC*SWAQAVCPP*VGPEGCVPTGLQPG GAVLSHLQAPAQGWHPAALHLLPPGPRGEWPGR WDRPETECTGVP*GHGGRPLQRALHPSRLPGE* GSQVQPALTLLHDDCRHPGLPEP*AGWVSPRSLL *GAVPQLHPRPAAEALPRAHLRAGVGKIQGGEH RAGV*RLGTPDG*LCGCCGPGLPSVPGPLAGPH RRGEGPALAIGRGGSGARGQ*GHPPGAPFLLLWP PGR*QKRPKPPSAQQQTTPLSPGPQPWHQLGRV QCDWLAELHQAEPSHPECPPAPAGLPEKNHQQP VSGPRRQCHGALWLHRGPGGRLAAGTAPGHQHAE NLYHRHGGCQKEVTVHPDEATGGRPHRHHQEVK AAFCALLPACS*GLGWGAPFRLLPPSQQQQ*AG PALGRPLRGWAPAARRAPAPHPAPRLPPARCHAH VPPRLP*PHGVFRVPPPL*CPGPAPDQETRA*L HRGG*KAGSGGAAGVLGSGEEQLLHGPEPGVLP GGHLGTAGGAAG*TNQQEPNPVPSSLQGLPGPPA LQEEKDPGPGHSLCTEEHQEEQRGEGLALVEAF YHSEAPHRSTAVRGADPEQRRGDPAAAEQAREG GEGEERAAAQQ*PAGEPDLRADIGADR*A*HRRV RLPAAGRGDSREAPG*EGDEGTADPVRCTEEAD GGYGNGGDGGPSHPGSGDQRGSG***CRWRVAA EV*AGCAGGGLHQETAPAGV*GQAGGGAAEQEAA GTAARGPAGR**GESAGSAAAQEEVPATDG*AA RHQAAPGGPAGPQPRTGEEAEEV*Q*ALAGA*G GPAGEAAAGEAAAGEGHAPR*GFQPEAATRGKRH GHCRVHPEGCVSRGRAPGHFFPRVQG*GFSGQG QETAPGPGGQSQGSGRRAG*AGRDHPDAGTGQA ASGDGDGADETDPF*GDGESG*GGGGGPAVVSEE VKTDGGAARGRV*GQAEGSAREAGAGGQARHPQ RPGEPAGL*VREAAAEGPEAHQGPAGRCPAHAG PPEEQCSQQARDCPAQEPAGGVRVHLCGSRESTE SNGGGDRRPAPAD**HRQSQDSAGGAAEPPSA* EE*DPEPAGGRSGRHERIDEEAQGCRGSGFPGP GSDK*SPSSARRSQQREAGAAGEATSPPEPGGVP GAVHGGQVPGEQAGS*DTGAGDTPGV*KDASET AGEPG*PSQGKHGEAD*GAGSAHCSREPGEGTE QAATEAAPGHQGGDGRACQEGGRGEPQEARTGDG SRKPGGC*PEPAG*PKVGIQAHRGPAGCH*G*D GE**E*GPHQQ*GRL*CGLGAGGPC*RGQVLVV KKQGTFQGSF**WQLKEFQPHQLLEVPCP*SVR* *ARPSRQHLQTAILPQLSE*QRHRGQADGD*RI AQGSGWQPSHPRACGCLGTSPRKWWGTGLPHPT ADLHGKHPDLLLSGALSRLWVSDVSTWKRWGKEE FLKRTFCSSVSKCQTLGFYPVSPWAVAGGLALH GASTLTSLSAPCSGTVDRLPTGTL*VAGSMGRI GGGEAVPSLSCGVGALSSWCLLSFYFLI*IPNLS ITAASLRVGGGCSGSWGSQERLGNVISIFTLQR AVLSLCSWRRW*GEPGPGS*E*GAEGLSGAAGL G*ASLRRQVCVWTIRTSSPHPTLFVSACYVQ*PI *GKPIPTTAVQG*SKHCLPPALSRWSGP*TQLE REGLGLRTCDQKN*RWKFWPVLIRHESSLCVLS PCHSSNLPAPTHLSQTQHHVVWRSQPPP*KGYP* ELHQTWEPKCSAWCCSHLPPPFSPAIGLYSLGC ALPCLPDVWK*RPFSSWLRQ

Frame 1

AAPRCALLHP*PGRGCWMPRLRFCCLPGGLRWP QQSGAPRPCAKHS*SSGGATPTNSTPTMFNLMK KDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMS LRRGFFNLNRSSKRESKTRLEISNPIPIKVASG SDLHLTDIDSDSNRGSVILDSGHLSTASSSDDL KGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS FSQRSRDESASETSTPSEHSAAPSPQVEVRTLE GQLVQHPGPGIPRPGHRSRAPELVTKKFPVDLR LPPVVPLPPPTLRELELQRRPTGDFGFSLRRTTM LDRGPEGQACRRVVHFAEPGAGTKDLALGLVPG DRLVEINGHNVESKSRDEIVEMIRQSGDSVRLK VQPIPELSELSRSWLRSGEGPRREPSDAKTEEQI AAEEAWNETEKVWLVHRDGFSLASQLKSEELNL PEGKVRVKLDHDGAILDVDEDDVEKANAPSCDR LEDLASLVYLNESSVLHTLRQRYGASLLHTYAGP SLLVLGPRGAPAVYSEKVMHMFKGCRREDMAPH IYAVAQTAYRAMLMSRQDQSIILLGSSGSGKTT SCQHLVQYLATIAGISGNKVFSVEKWQALYTLLE AFGNSPTIINGNATRFSQILSLDFDQAGQVASA SIQTMLLEKLRVARRPASEATFNVFYYLLACGD GTLRTELHLNHLAENNVFGIVPLAKPEEKQKAAQ QFSKLQAAMKVLGISPDEQKACWFILAAIYHLG AAGATKEAAEAGRKQFARHEWAQKAAYLLGCSL EELSSAIFKHQHKGGTLQRSTSFRQGPEESGLGD GTGPKLSALECLEGMAAGLYSELFTLLVSLVNR ALKSSQHSLCSMMIVDTPGFQNPEQGGSARGAS FEELCHNYTQDRLQRLFHERTFVQELERYKEENI ELAFDDLEPPTDDSVAAVDQASHQSLVRSLART DEARGLLWLLEEEALVPGASEDTLLERLFSYYG PQEGDKKGQSPLLHSSKPHHFLLGHSHGTNWVEY NVTGWLNYTKQNPATQNAPRLLQDSQKKIISNL FLGRAGSATVLSGSIAGLEGGSQLALRRATSMR KTFTTGMAAVKKKSLCIQMKLQVDALIDTIKKSK LHFVHCFLPVAEGWAGEPRSASSRRVSSSSELD LPSGDHCEAGLLQLDVPLLRTQLRGSRLLDAMR MYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRNY IVVDERRAVEELLECLDLEKSSCCMGLSRVFFR AGTLARLEEQRDEQTSRNLTLFQAACRGYLARQ HFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLF TTVRPLIEVQLSEEQIRNKDEEIQQLRSKLEKA EKERNELRLNSDRLESRISELTSELTDERNTGE SASQLLDAETAERLRAEKEMKELQTQYDALKKQM EVMEMEVMEARLIRAAEINGEVDDDDAGGEWRL KYERAVREVDFTKKRLQQEFEDKLEVEQQNKRQ LERRLGDLQADSEESQRALQQLKKKCQRLTAELQ DTKLHLEGQQVRNHELEKKQRRFDSELSQAHEE AQREKLQREKLQREKDMLLAEAFSLKQQLEEKD MDIAGFTQKVVSLEAELQDISSQESKDEASLAKV KKQLRDLEAKVKDQEEELDEQAGTIQMLEQAKL RLEMEMERMRQTHSKEMESRDEEVEEARQSCQK KLKQMEVQLEEEYEDKQKVLREKRELEGKLATLS DQVNRRDFESEKRLRKDLKRTKALLADAQLMLD HLKNSAPSKREIAQLKNQLEESEFTCAAAVKAR KAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQRE KNEIQNRLEEDQEDMNELMKKHKAAVAQASRDL AQINDLQAQLEEANKEKQELQEKLQALQSQVEF LEQSMVDKSLVSRQEAKIRELETRLEFERTQVKR LESLASRLKENMEKLTEERDQRIAAENREKEQN KRLQRQLRDTKEEMGELARKEAEASRKKHELEM DLESLEAANQSLQADLKLAFKRIGDLQAAIEDEM ESDENEDLINSEGDSDVDSELEDRVDGVKSWLS KNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSD DEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA* PRGVVGSPLTPGPVAAWAPLPGSGGAPVSPTRL LICMGNTLTFFCQGHFPGYGCLMSPRGRGGGKR SF*RELFAPLSQNARLLASTLCHRGQWQVAWHCM EPAR*PPSQLPAQGRWTGCLLGHSRLLGPWGGL GEEKQCLPSRVGWGLSLLGACCLSTF*FKYPTS PSQLHP*EWEGAVVVAGAPKNDSGMSSPSSPFRE QSFLCAAGDAGEESRVQVLKNEVRRGSPVLLGW VEQAYADKCVCGPSAPPAPTPPSLSQRVMCNDL FKVNPFQLQQFRADPSTASLLLCPGGLDHKLNLR GKAWG*GLVIRKTEDGSFGRCSLDMSPHSVS*A RVILPTSLPPHTYPRHNTMWSGGPSPHPKKVIP ENSTRLGSPSAVPGAAPICRPPSLLQLVCTHWAV LSPVYPMYGNKGPFPPGCAS

Frame 2

LRRAALCSIPDRAEAAGCRVSASAACRAGSGGR SKVGHQGPVLSTHNPLGVLPLQTAPPPCLT**R KTRTKMAGGRRRRRKRRKRSGCQRQSFGAWRR* ACDVASST*TAPPSVNPRRAWKSPTPSPSRWPAA LTCT*LTLTPIVTGAASSWTRAT*VQPAPAMTS RVRRVASVARCCSGQPSSAHWPSRTHR*LSSAF PSPSVAGMRAPQKPRRPQSTLPPPRHRWR*GL*R DSWCSILAQASLDQGTDPEPLS**LKSSQSTCA CPPWCPCPHLPSGSWSCNDGPLETLASPCGAQP CWIGAPRARPVGVWSTLLSLVQAPRTWPWGWCQE IDWWRLMGTMWRASPGMRLWR*SGSQGTACGSR CSPFQSSASSAGAGCGAARDLAGSHPMRKQKNR LQQKRPGMRRRRCGWSIGTASHWPVNSNLRSSTC LRGRCV*SWTTMGPSWMWMRMTLRRLMLPPATV WRIWPHWCTSMSPASCTPCASAMALACCTRMLA PACWFLAPVGPLLCTLRR*CTCSRVVGGRTWHPT SMQWPRPHTGRC**AVRISQSSSWAVVAVARPP AASIWCSTWPPSRASAGTRCFLWRSGRLCTPSW KPLGTAPPSLMAMPPASPRSSPWTLTKLARWPQP PFRQCFWRSCVWLGAQPVKPHSTSSTTCWPVGM APSGQSSTSTTWQRTMCLGLCHWPSLRKSRRQL SSLVSCRRP*RCWASPPMNRRPAGSFWLPSTTWG LREPPKKLLKLGASSLPAMSGPRRLRTYWAAAW RSCPQPSSSTSTRVAPCSAPPPSARAPRRVAWE MGQARN*VHWSALRAWRPASTASSSPFSSPW*IG LSSPASTHSAP**LSTPRASRTLSRVGQPAEPP LRSCATTTPKTGCRGSSTSAPSCRSWKDTRRRT SSWRLTTWNPRRMTLWLLWTRPPISPWSARWPAQ TRRGACSGYWKRRLWCQGPVRTPSWSAFSPIMA PRKVTKKAKAPFCTAANHTTFSWATAMAPTG*S TM*LAG*TTPSRTQPPRMPPGSCRTPRKKSSATC FWAAQAVPRCSLAPSRAWRAARSWHCAGPPACG KPLPQAWRLSKRSHCASR*SYRWTPSSTPSRSQ SCILCTASCL*LRAGLGSPVPPPPAESAAAVSWT CPRETTARLGSCSSTCPCSAPSSAAPACSMPCA CTAKVTLTTWCFPSSAAALMSWPRT*PRNTGVT TSWWMKGGQWRSCWSAWIWRRAAAAWA*AGCSSG RAPWHGWRSSGMNKPAGT*PCSKQPAGATWPAS TSRRERSRTWPFAVYRRTSRRTKG*RTGPGGSF LPQ*GPSSKYSCQRSRSGTKTRRSSSCGASSRRR RRRGTSCGSTVTGWRAGSQS*HRS*QMSVTQES PPPSCWTRRQQRGSGLRRR*RNCRPSTMH*RSR WRLWKWR*WRPVSSGQRRSTGKWMMMMQVASGG* SMSGLCGRWTSPRNGSSRSLRTSWRWSSRTRGS WNGGSGTCRQIVRRVSGLCSSSRRSASD*RLSC KTPSCTWRASRSATTNWRRSRGGLTVSSRRRMRR PSGRSCSGRSCSGRRTCSSLRLSA*SSN*RKKT WTLQGSPRRLCL*RQSSRTFLPKSPRMRLLWPR SRNSSGTWRPKSRIRKKSWMSRQGPSRCWNRPSC VWRWRWSG*DRPILRRWRVGMRRWRRPGSRVRR S*NRWRCS*RKSMRTSRRFCERSGSWRASSPPS ATR*TGGTLSQRSGCGRT*SAPRPCWQMPSSCWT T*RTVLPASERLPSSRTSWRSQSSPVRQP*KHG KQWRWRSKTCTCRLMTSPKPRQRWRSS*AAFSV RRMRSRTGWRKIRKT*TN**RSTRLPWLRLPGTW LR*MISKLS*KKPTKRSRSCRRSYKPSRARWSS WSSPWWTSPW*AGRKLRYGSWRHAWSLKGRK*N GWRAWLAVSRKTWRS*LRSGISALQPRTGRRNRT SGYRGSSGTPRRRWASLPGRRPRRAARSTNWRW I*KAWRLLTRACRLT*SWHSSASGTCRLPLRMR WRVMRMRTSSTVRETLMWTRSWRTVLTGSSPGCQ KTRDLPRQLLMMAA*RVPAPPATGSPLPLIGQM MSTTLSTTPPDRDTPTVI*VTATQRPS*RRLTH SPGEWLAALSPQGLWLPGHLSQEVVGHRSPPPDC *SAWETP*PSSVRGTFQAMGV*CLHVEEVGERG VSEENFLLLCLKMPDSWLLPCVTVGSGRWPGTA WSQHVDLPLSSLLRDGGQVAYWDTLGCWVHGEDW GRRSSAFPLVWGGGSLFLVPAVFLLFNLNTQPL HHSCIPESGRGL*W*LGLPRTTRECHLHLHPSE SSPFSVQLETLVRRAGSRFLRMRCGGALRCCWAG LSKPTQTSVCVDHPHLQPPPHPLCLSVLCAMTY LR*THSNYSSSGLIQALPPSCSVQVVWTINST* EGRLGVEDL*SEKLKMEVLAGAH*T*VLTLCPEP VSFFQPPCPHTLIPDTTPCGLEVPAPTLKRLSL RTPPDLGAQVQCLVLLPSAAPLLSCNWFVLTGL CSPLFTRCMEIKALFLLAAP

Sequence TIAF1.aAug05

AGCTGCGCCGCGCTGCGCTCTGCTCCATCCCTGACCGGGCCGAGGCTGCTGGATGCCGCGTCTCCGCTTCTGCTGCCTGCCGGGCGGGCTCCGGTGGCCG CAGCAAAGTGGGGCACCAAGGCCCTGTGCTAAGCACTCATAATCCTCTGGGGGTGCTACCCCTACAAACAGCACCCCCACCATGTTTAACCTAATGAAGA AAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGAAAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAG CCTGCGACGTGGCTTCTTCAACCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCCATCAAGGTGGCCAGCGGC TCTGACCTGCACCTGACTGACATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACTCGGGCCACCTAAGTACAGCCAGCTCCAGCGATGACCTCA AGGGTGAGGAGGGTAGCTTCCGTGGCTCGGTGCTGCAGCGGGCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTC CTTCTCCCAGCGTAGCCGGGATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCTGCCGCCCCCTCGCCACAGGTGGAGGTGAGGACTCTAGAG GGACAGCTGGTGCAGCATCCTGGCCCAGGCATCCCTCGACCAGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAGTTCCCAGTCGACCTGCGCC TGCCCCCCGTGGTGCCCCTGCCCCCACCTACCCTCCGGGAGCTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCTCCCTGCGGCGCACAACCAT GCTGGATCGGGGCCCCGAGGGCCAGGCCTGTCGGCGTGTGGTCCACTTTGCTGAGCCTGGTGCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGA GATCGACTGGTGGAGATTAATGGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGGGACAGCGTGCGGCTCAAGG TGCAGCCCATTCCAGAGCTCAGCGAGCTCAGCAGGAGCTGGCTGCGGAGCGGCGAGGGACCTCGCAGGGAGCCATCCGATGCGAAAACAGAAGAACAGAT TGCAGCAGAAGAGGCCTGGAATGAGACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCTGAGGAGCTCAACTTG CCTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATCCTGGATGTGGATGAGGATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTC TGGAGGATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCAGCGCTATGGCGCTAGCCTGCTGCACACGTATGCTGGCCC CAGCCTGCTGGTTCTTGGCCCCCGTGGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCGGGAGGACATGGCACCCCAC ATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGCCGTCAGGATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCA GCTGCCAGCATCTGGTGCAGTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCTGTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGA AGCCTTTGGGAACAGCCCCACCATCATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCCTCAGCC TCCATTCAGACAATGCTTCTGGAGAAGCTGCGTGTGGCTCGGCGCCCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATG GCACCCTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCACTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCA GCAGTTTAGTAAGCTGCAGGCGGCCATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCCATCTACCACCTGGGG GCTGCGGGAGCCACCAAAGAAGCTGCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTACCTACTGGGCTGCAGCCTGG AGGAGCTGTCCTCAGCCATCTTCAAGCACCAGCACAAGGGTGGCACCCTGCAGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGA TGGGACAGGCCCGAAACTGAGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGCGAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGG GCTCTCAAGTCCAGCCAGCACTCACTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCCAGAACCCTGAGCAGGGTGGGTCAGCCCGCGGAGCCTCCT TTGAGGAGCTGTGCCACAACTACACCCAAGACCGGCTGCAGAGGCTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACAT CGAGCTGGCGTTTGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCCTCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACA GACGAGGCGAGGGGCCTGCTCTGGCTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTTTTCTCCTATTATGGCC CCCAGGAAGGTGACAAAAAAGGCCAAAGCCCCCTTCTGCACAGCAGCAAACCACACCACTTTCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTA CAATGTGACTGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGGCTCCTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTG TTTCTGGGCCGCGCAGGCAGTGCCACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGGGCCACCAGCATGCGGA AAACCTTTACCACAGGCATGGCGGCTGTCAAAAAGAAGTCACTGTGCATCCAGATGAAGCTACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAA GCTGCATTTTGTGCACTGCTTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCCTCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGAC CTGCCCTCGGGAGACCACTGCGAGGCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCGCGGCTCCCGCCTGCTCGATGCCATGCGCA TGTACCGCCAAGGTTACCCTGACCACATGGTGTTTTCCGAGTTCCGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTA CATCGTGGTGGATGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGCAGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGG GCGGGCACCTTGGCACGGCTGGAGGAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCCTGTTCCAAGCAGCCTGCAGGGGCTACCTGGCCCGCCAGC ACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTACAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTT TACCACAGTGAGGCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGACGAGGAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCG GAGAAGGAGAGGAACGAGCTGCGGCTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAGCGTAACACAGGAGAGT CCGCCTCCCAGCTGCTGGACGCGGAGACAGCAGAGAGGCTCCGGGCTGAGAAGGAGATGAAGGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGAT GGAGGTTATGGAAATGGAGGTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGGGAAGTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTG AAGTATGAGCGGGCTGTGCGGGAGGTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAGCAGCAGAACAAGAGGCAGC TGGAACGGCGGCTCGGGGACCTGCAGGCAGATAGTGAGGAGAGTCAGCGGGCTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCA AGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAG GCCCAGCGGGAGAAGCTGCAGCGGGAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTGAGGCTTTCAGCCTGAAGCAGCAACTAGAGGAAAAAGACA TGGACATTGCAGGGTTCACCCAGAAGGTTGTGTCTCTAGAGGCAGAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGT CAAGAAACAGCTCCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAGGCAGGGACCATCCAGATGCTGGAACAGGCCAAGCTG CGTCTGGAGATGGAGATGGAGCGGATGAGACAGACCCATTCTAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAGGAGGCCCGGCAGTCGTGTCAGAAGA AGTTAAAACAGATGGAGGTGCAGCTAGAGGAAGAGTATGAGGACAAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAG CGACCAGGTGAACCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGACCTGAAGCGCACCAAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGAC CACCTGAAGAACAGTGCTCCCAGCAAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCAGCCGTGAAAGCACGGA AAGCAATGGAGGTGGAGATCGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGA GAAGAATGAGATCCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAGAAGCACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTG GCTCAGATAAATGATCTCCAAGCTCAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAGAGCCAGGTGGAGTTCC TGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAGGAAGCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACG GCTGGAGAGCCTGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAGCGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAAC AAGCGGCTACAGAGGCAGCTCCGGGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAGCACGAACTGGAGATGG ATCTAGAAAGCCTGGAGGCTGCTAACCAGAGCCTGCAGGCTGACCTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGAT GGAGAGTGATGAGAATGAGGACCTCATCAACAGTTTGCAGGACATGGTGACAAAGTATCAGAAAAGAAAGAATAAACTTGAGGGAGACTCTGATGTGGAC TCGGAGCTGGAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCAGCTTCTGATGATGGCAGCTTAAAGAGTTCCA GCCCCACCAGCTACTGGAAGTCCCTTGCCCCTGATCGGTCAGATGATGAGCACGACCCTCTCGACAACACCTCCAGACCGCGATACTCCCACAGTTATCT GAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATAGCCCAGGGGAGTGGTTGGCAGCCCTCTCACCCCAGGGCCTGTGGCTGCCTGGGCA CCTCTCCCAGGAAGTGGTGGGGCACCGGTCTCCCCCACCCGACTGCTGATCTGCATGGGAAACACCCTGACCTTCTTCTGTCAGGGGCACTTTCCAGGCT ATGGGTGTCTGATGTCTCCACGTGGAAGAGGTGGGGGAAAGAGGAGTTTCTGAAGAGAACTTTTTGCTCCTCTGTCTCAAAATGCCAGACTCTTGGCTTC TACCCTGTGTCACCGTGGGCAGTGGCAGGTGGCCTGGCACTGCATGGAGCCAGCACGTTGACCTCCCTCTCAGCTCCCTGCTCAGGGACGGTGGACAGGT TGCCTACTGGGACACTCTAGGTTGCTGGGTCCATGGGGAGGATTGGGGGAGGAGAAGCAGTGCCTTCCCTCTCGTGTGGGGTGGGGGCTCTCTCTTCTTG GTGCCTGCTGTCTTTCTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGCATCCCTGAGAGTGGGAGGGGGCTGTAGTGGTAGCTGGGGCTCC CAAGAACGACTCGGGAATGTCATCTCCATCTTCACCCTTCAGAGAGCAGTCCTTTCTCTGTGCAGCTGGAGACGCTGGTGAGGAGAGCCGGGTCCAGGTT CTTAAGAATGAGGTGCGGAGGGGCTCTCCGGTGCTGCTGGGCTGGGTTGAGCAAGCCTACGCAGACAAGTGTGTGTGTGGACCATCCGCACCTCCAGCCC CCACCCCACCCTCTTTGTCTCAGCGTGTTATGTGCAATGACCTATTTAAGGTAAACCCATTCCAACTACAGCAGTTCAGGGCTGATCCAAGCACTGCCTC CCTCCTGCTCTGTCCAGGTGGTCTGGACCATAAACTCAACTTGAGAGGGAAGGCTTGGGGTTGAGGACTTGTGATCAGAAAAACTGAAGATGGAAGTTTT GGCCGGTGCTCATTAGACATGAGTCCTCACTCTGTGTCCTGAGCCCGTGTCATTCTTCCAACCTCCCTGCCCCCACACACTTATCCCAGACACAACACCA TGTGGTCTGGAGGTCCCAGCCCCCACCCTAAAAAGGTTATCCCTGAGAACTCCACCAGACTTGGGAGCCCAAGTGCAGTGCCTGGTGCTGCTCCCATCTG CCGCCCCCCTTCTCTCCTGCAATTGGTTTGTACTCACTGGGCTGTGCTCTCCCCTGTTTACCCGATGTATGGAAATAAAGGCCCTTTTCCTCCTGGCTGC GCCAGT

TIAF1-3 (forward primer)
TIAF1-4 (reverse primer)
Single exon skipping

Frame 0

SCAALRSAPSLTGPRLLDAASPLLLPAGRAPVA AAKWGTKALC*ALIILWGCYPYKQHPHHV*PNEE RQGQRWRAEGEEGEKGEKGADVSGRASEPGGDE PATWLLQPEPLLQA*IQDAPGNLQPHPHQGGQR L*PAPD*H*LR**PGQRHPGLGPPKYSQLQR*PQ G*GG*LPWLGAAAGSQVRLTGQAELTDDCQALF LLPA*PG*ERLRNLDALRALCRPLATGGGEDSR GTAGAASWPRHPSTRAPIPSP*ASD*KVPSRPAP APRGAPAPTYPPGAGAATTAHWRLWLLPAAHNH AGSGPRGPGLSACGPLC*AWCRHQGPGPGAGAR RSTGGD*WAQCGEQVQG*DCGDDPAVRGQRAAQG AAHSRAQRAQQELAAERRGTSQGAIRCENRRTD CSRRGLE*DGEGVAGP*GRLLTGQSTQI*GAQL A*GEGACEAGPRWGHPGCG*G*R*EG*CSLLRPS GGSGLTGVPQ*VQRPAHLAPALWR*PAAHVCWP QPAGSWPPWGPCCVL*EGDAHVQGLSAGGHGTP HLCSGPDRIQGDADEPSGSVNHPPGQ*WQWQDHQ LPASGAVPGHHRGHQREQGVFCGEVAGSVHPPG SLWEQPHHH*WQCHPLLPDPLPGL*PSWPGGLS LHSDNASGEAACGSAPSQ*SHIQRLLLPAGLWGW HPQDRAPPQPLGREQCVWDCATGQA*GKAEGSS AV**AAGGHEGAGHLPR*TEGLLVHSGCHLPPG GCGSHQRSC*SWAQAVCPP*VGPEGCVPTGLQPG GAVLSHLQAPAQGWHPAALHLLPPGPRGEWPGR WDRPETECTGVP*GHGGRPLQRALHPSRLPGE* GSQVQPALTLLHDDCRHPGLPEP*AGWVSPRSLL *GAVPQLHPRPAAEALPRAHLRAGVGKIQGGEH RAGV*RLGTPDG*LCGCCGPGLPSVPGPLAGPH RRGEGPALAIGRGGSGARGQ*GHPPGAPFLLLWP PGR*QKRPKPPSAQQQTTPLSPGPQPWHQLGRV QCDWLAELHQAEPSHPECPPAPAGLPEKNHQQP VSGPRRQCHGALWLHRGPGGRLAAGTAPGHQHAE NLYHRHGGCQKEVTVHPDEATGGRPHRHHQEVK AAFCALLPACS*GLGWGAPFRLLPPSQQQQ*AG PALGRPLRGWAPAARRAPAPHPAPRLPPARCHAH VPPRLP*PHGVFRVPPPL*CPGPAPDQETRA*L HRGG*KAGSGGAAGVLGSGEEQLLHGPEPGVLP GGHLGTAGGAAG*TNQQEPNPVPSSLQGLPGPPA LQEEKDPGPGHSLCTEEHQEEQRGEGLALVEAF YHSEAPHRSTAVRGADPEQRRGDPAAAEQAREG GEGEERAAAQQ*PAGEPDLRADIGADR*A*HRRV RLPAAGRGDSREAPG*EGDEGTADPVRCTEEAD GGYGNGGDGGPSHPGSGDQRGSG***CRWRVAA EV*AGCAGGGLHQETAPAGV*GQAGGGAAEQEAA GTAARGPAGR**GESAGSAAAQEEVPATDG*AA RHQAAPGGPAGPQPRTGEEAEEV*Q*ALAGA*G GPAGEAAAGEAAAGEGHAPR*GFQPEAATRGKRH GHCRVHPEGCVSRGRAPGHFFPRVQG*GFSGQG QETAPGPGGQSQGSGRRAG*AGRDHPDAGTGQA ASGDGDGADETDPF*GDGESG*GGGGGPAVVSEE VKTDGGAARGRV*GQAEGSAREAGAGGQARHPQ RPGEPAGL*VREAAAEGPEAHQGPAGRCPAHAG PPEEQCSQQARDCPAQEPAGGVRVHLCGSRESTE SNGGGDRRPAPAD**HRQSQDSAGGAAEPPSA* EE*DPEPAGGRSGRHERIDEEAQGCRGSGFPGP GSDK*SPSSARRSQQREAGAAGEATSPPEPGGVP GAVHGGQVPGEQAGS*DTGAGDTPGV*KDASET AGEPG*PSQGKHGEAD*GAGSAHCSREPGEGTE QAATEAAPGHQGGDGRACQEGGRGEPQEARTGDG SRKPGGC*PEPAG*PKVGIQAHRGPAGCH*G*D GE**E*GPHQQFAGHGDKVSEKKE*T*GRL*CG LGAGGPC*RGQVLVVKKQGTFQGSF**WQLKEFQ PHQLLEVPCP*SVR**ARPSRQHLQTAILPQLS E*QRHRGQADGD*RIAQGSGWQPSHPRACGCLG TSPRKWWGTGLPHPTADLHGKHPDLLLSGALSRL WVSDVSTWKRWGKEEFLKRTFCSSVSKCQTLGF YPVSPWAVAGGLALHGASTLTSLSAPCSGTVDR LPTGTL*VAGSMGRIGGGEAVPSLSCGVGALSSW CLLSFYFLI*IPNLSITAASLRVGGGCSGSWGS QERLGNVISIFTLQRAVLSLCSWRRW*GEPGPG S*E*GAEGLSGAAGLG*ASLRRQVCVWTIRTSSP HPTLFVSACYVQ*PI*GKPIPTTAVQG*SKHCL PPALSRWSGP*TQLEREGLGLRTCDQKN*RWKF WPVLIRHESSLCVLSPCHSSNLPAPTHLSQTQHH VVWRSQPPP*KGYP*ELHQTWEPKCSAWCCSHL PPPFSPAIGLYSLGCALPCLPDVWK*RPFSSWL RQ

Frame 1

AAPRCALLHP*PGRGCWMPRLRFCCLPGGLRWP QQSGAPRPCAKHS*SSGGATPTNSTPTMFNLMK KDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMS LRRGFFNLNRSSKRESKTRLEISNPIPIKVASG SDLHLTDIDSDSNRGSVILDSGHLSTASSSDDL KGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS FSQRSRDESASETSTPSEHSAAPSPQVEVRTLE GQLVQHPGPGIPRPGHRSRAPELVTKKFPVDLR LPPVVPLPPPTLRELELQRRPTGDFGFSLRRTTM LDRGPEGQACRRVVHFAEPGAGTKDLALGLVPG DRLVEINGHNVESKSRDEIVEMIRQSGDSVRLK VQPIPELSELSRSWLRSGEGPRREPSDAKTEEQI AAEEAWNETEKVWLVHRDGFSLASQLKSEELNL PEGKVRVKLDHDGAILDVDEDDVEKANAPSCDR LEDLASLVYLNESSVLHTLRQRYGASLLHTYAGP SLLVLGPRGAPAVYSEKVMHMFKGCRREDMAPH IYAVAQTAYRAMLMSRQDQSIILLGSSGSGKTT SCQHLVQYLATIAGISGNKVFSVEKWQALYTLLE AFGNSPTIINGNATRFSQILSLDFDQAGQVASA SIQTMLLEKLRVARRPASEATFNVFYYLLACGD GTLRTELHLNHLAENNVFGIVPLAKPEEKQKAAQ QFSKLQAAMKVLGISPDEQKACWFILAAIYHLG AAGATKEAAEAGRKQFARHEWAQKAAYLLGCSL EELSSAIFKHQHKGGTLQRSTSFRQGPEESGLGD GTGPKLSALECLEGMAAGLYSELFTLLVSLVNR ALKSSQHSLCSMMIVDTPGFQNPEQGGSARGAS FEELCHNYTQDRLQRLFHERTFVQELERYKEENI ELAFDDLEPPTDDSVAAVDQASHQSLVRSLART DEARGLLWLLEEEALVPGASEDTLLERLFSYYG PQEGDKKGQSPLLHSSKPHHFLLGHSHGTNWVEY NVTGWLNYTKQNPATQNAPRLLQDSQKKIISNL FLGRAGSATVLSGSIAGLEGGSQLALRRATSMR KTFTTGMAAVKKKSLCIQMKLQVDALIDTIKKSK LHFVHCFLPVAEGWAGEPRSASSRRVSSSSELD LPSGDHCEAGLLQLDVPLLRTQLRGSRLLDAMR MYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRNY IVVDERRAVEELLECLDLEKSSCCMGLSRVFFR AGTLARLEEQRDEQTSRNLTLFQAACRGYLARQ HFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLF TTVRPLIEVQLSEEQIRNKDEEIQQLRSKLEKA EKERNELRLNSDRLESRISELTSELTDERNTGE SASQLLDAETAERLRAEKEMKELQTQYDALKKQM EVMEMEVMEARLIRAAEINGEVDDDDAGGEWRL KYERAVREVDFTKKRLQQEFEDKLEVEQQNKRQ LERRLGDLQADSEESQRALQQLKKKCQRLTAELQ DTKLHLEGQQVRNHELEKKQRRFDSELSQAHEE AQREKLQREKLQREKDMLLAEAFSLKQQLEEKD MDIAGFTQKVVSLEAELQDISSQESKDEASLAKV KKQLRDLEAKVKDQEEELDEQAGTIQMLEQAKL RLEMEMERMRQTHSKEMESRDEEVEEARQSCQK KLKQMEVQLEEEYEDKQKVLREKRELEGKLATLS DQVNRRDFESEKRLRKDLKRTKALLADAQLMLD HLKNSAPSKREIAQLKNQLEESEFTCAAAVKAR KAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQRE KNEIQNRLEEDQEDMNELMKKHKAAVAQASRDL AQINDLQAQLEEANKEKQELQEKLQALQSQVEF LEQSMVDKSLVSRQEAKIRELETRLEFERTQVKR LESLASRLKENMEKLTEERDQRIAAENREKEQN KRLQRQLRDTKEEMGELARKEAEASRKKHELEM DLESLEAANQSLQADLKLAFKRIGDLQAAIEDEM ESDENEDLINSLQDMVTKYQKRKNKLEGDSDVD SELEDRVDGVKSWLSKNKGPSKAASDDGSLKSS SPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYL SDSDTEAKLTETNA*PRGVVGSPLTPGPVAAWA PLPGSGGAPVSPTRLLICMGNTLTFFCQGHFPG YGCLMSPRGRGGGKRSF*RELFAPLSQNARLLAS TLCHRGQWQVAWHCMEPAR*PPSQLPAQGRWTG CLLGHSRLLGPWGGLGEEKQCLPSRVGWGLSLL GACCLSTF*FKYPTSPSQLHP*EWEGAVVVAGAP KNDSGMSSPSSPFREQSFLCAAGDAGEESRVQV LKNEVRRGSPVLLGWVEQAYADKCVCGPSAPPA PTPPSLSQRVMCNDLFKVNPFQLQQFRADPSTAS LLLCPGGLDHKLNLRGKAWG*GLVIRKTEDGSF GRCSLDMSPHSVS*ARVILPTSLPPHTYPRHNT MWSGGPSPHPKKVIPENSTRLGSPSAVPGAAPIC RPPSLLQLVCTHWAVLSPVYPMYGNKGPFPPGC AS

Frame 2

LRRAALCSIPDRAEAAGCRVSASAACRAGSGGR SKVGHQGPVLSTHNPLGVLPLQTAPPPCLT**R KTRTKMAGGRRRRRKRRKRSGCQRQSFGAWRR* ACDVASST*TAPPSVNPRRAWKSPTPSPSRWPAA LTCT*LTLTPIVTGAASSWTRAT*VQPAPAMTS RVRRVASVARCCSGQPSSAHWPSRTHR*LSSAF PSPSVAGMRAPQKPRRPQSTLPPPRHRWR*GL*R DSWCSILAQASLDQGTDPEPLS**LKSSQSTCA CPPWCPCPHLPSGSWSCNDGPLETLASPCGAQP CWIGAPRARPVGVWSTLLSLVQAPRTWPWGWCQE IDWWRLMGTMWRASPGMRLWR*SGSQGTACGSR CSPFQSSASSAGAGCGAARDLAGSHPMRKQKNR LQQKRPGMRRRRCGWSIGTASHWPVNSNLRSSTC LRGRCV*SWTTMGPSWMWMRMTLRRLMLPPATV WRIWPHWCTSMSPASCTPCASAMALACCTRMLA PACWFLAPVGPLLCTLRR*CTCSRVVGGRTWHPT SMQWPRPHTGRC**AVRISQSSSWAVVAVARPP AASIWCSTWPPSRASAGTRCFLWRSGRLCTPSW KPLGTAPPSLMAMPPASPRSSPWTLTKLARWPQP PFRQCFWRSCVWLGAQPVKPHSTSSTTCWPVGM APSGQSSTSTTWQRTMCLGLCHWPSLRKSRRQL SSLVSCRRP*RCWASPPMNRRPAGSFWLPSTTWG LREPPKKLLKLGASSLPAMSGPRRLRTYWAAAW RSCPQPSSSTSTRVAPCSAPPPSARAPRRVAWE MGQARN*VHWSALRAWRPASTASSSPFSSPW*IG LSSPASTHSAP**LSTPRASRTLSRVGQPAEPP LRSCATTTPKTGCRGSSTSAPSCRSWKDTRRRT SSWRLTTWNPRRMTLWLLWTRPPISPWSARWPAQ TRRGACSGYWKRRLWCQGPVRTPSWSAFSPIMA PRKVTKKAKAPFCTAANHTTFSWATAMAPTG*S TM*LAG*TTPSRTQPPRMPPGSCRTPRKKSSATC FWAAQAVPRCSLAPSRAWRAARSWHCAGPPACG KPLPQAWRLSKRSHCASR*SYRWTPSSTPSRSQ SCILCTASCL*LRAGLGSPVPPPPAESAAAVSWT CPRETTARLGSCSSTCPCSAPSSAAPACSMPCA CTAKVTLTTWCFPSSAAALMSWPRT*PRNTGVT TSWWMKGGQWRSCWSAWIWRRAAAAWA*AGCSSG RAPWHGWRSSGMNKPAGT*PCSKQPAGATWPAS TSRRERSRTWPFAVYRRTSRRTKG*RTGPGGSF LPQ*GPSSKYSCQRSRSGTKTRRSSSCGASSRRR RRRGTSCGSTVTGWRAGSQS*HRS*QMSVTQES PPPSCWTRRQQRGSGLRRR*RNCRPSTMH*RSR WRLWKWR*WRPVSSGQRRSTGKWMMMMQVASGG* SMSGLCGRWTSPRNGSSRSLRTSWRWSSRTRGS WNGGSGTCRQIVRRVSGLCSSSRRSASD*RLSC KTPSCTWRASRSATTNWRRSRGGLTVSSRRRMRR PSGRSCSGRSCSGRRTCSSLRLSA*SSN*RKKT WTLQGSPRRLCL*RQSSRTFLPKSPRMRLLWPR SRNSSGTWRPKSRIRKKSWMSRQGPSRCWNRPSC VWRWRWSG*DRPILRRWRVGMRRWRRPGSRVRR S*NRWRCS*RKSMRTSRRFCERSGSWRASSPPS ATR*TGGTLSQRSGCGRT*SAPRPCWQMPSSCWT T*RTVLPASERLPSSRTSWRSQSSPVRQP*KHG KQWRWRSKTCTCRLMTSPKPRQRWRSS*AAFSV RRMRSRTGWRKIRKT*TN**RSTRLPWLRLPGTW LR*MISKLS*KKPTKRSRSCRRSYKPSRARWSS WSSPWWTSPW*AGRKLRYGSWRHAWSLKGRK*N GWRAWLAVSRKTWRS*LRSGISALQPRTGRRNRT SGYRGSSGTPRRRWASLPGRRPRRAARSTNWRW I*KAWRLLTRACRLT*SWHSSASGTCRLPLRMR WRVMRMRTSSTVCRTW*QSIRKERINLRETLMWT RSWRTVLTGSSPGCQKTRDLPRQLLMMAA*RVP APPATGSPLPLIGQMMSTTLSTTPPDRDTPTVI *VTATQRPS*RRLTHSPGEWLAALSPQGLWLPGH LSQEVVGHRSPPPDC*SAWETP*PSSVRGTFQA MGV*CLHVEEVGERGVSEENFLLLCLKMPDSWL LPCVTVGSGRWPGTAWSQHVDLPLSSLLRDGGQV AYWDTLGCWVHGEDWGRRSSAFPLVWGGGSLFL VPAVFLLFNLNTQPLHHSCIPESGRGL*W*LGL PRTTRECHLHLHPSESSPFSVQLETLVRRAGSRF LRMRCGGALRCCWAGLSKPTQTSVCVDHPHLQP PPHPLCLSVLCAMTYLR*THSNYSSSGLIQALP PSCSVQVVWTINST*EGRLGVEDL*SEKLKMEVL AGAH*T*VLTLCPEPVSFFQPPCPHTLIPDTTP CGLEVPAPTLKRLSLRTPPDLGAQVQCLVLLPS AAPLLSCNWFVLTGLCSPLFTRCMEIKALFLLAA P